AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006894.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006894.01.T01 MTR_2g016160 85.443 316 3 2 1 273 1 316 0.0 533
MsG0280006894.01.T01 MTR_4g073080 25.328 229 161 4 47 266 99 326 5.33e-14 72.0
MsG0280006894.01.T01 MTR_4g119620 23.950 238 170 8 45 280 61 289 1.78e-13 70.1
MsG0280006894.01.T01 MTR_2g102277 28.395 162 110 4 78 238 73 229 3.33e-12 67.4
MsG0280006894.01.T01 MTR_2g102277 28.395 162 110 4 78 238 73 229 3.65e-12 67.0
MsG0280006894.01.T01 MTR_4g011950 27.778 162 111 4 78 238 73 229 1.26e-11 65.5
MsG0280006894.01.T01 MTR_5g016350 31.111 135 80 5 99 224 410 540 7.35e-11 62.8
MsG0280006894.01.T01 MTR_5g016350 31.111 135 80 5 99 224 346 476 9.64e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006894.01.T01 AT1G29260 68.454 317 54 4 1 273 1 315 5.80e-159 445
MsG0280006894.01.T01 AT4G35050 25.641 234 160 5 46 266 101 333 5.97e-13 68.9
MsG0280006894.01.T01 AT4G02730 28.000 225 145 12 61 280 85 297 6.87e-13 68.6
MsG0280006894.01.T01 AT3G15980 28.395 162 110 4 78 238 73 229 5.14e-12 66.6
MsG0280006894.01.T01 AT3G15980 28.395 162 110 4 78 238 73 229 5.22e-12 66.6
MsG0280006894.01.T01 AT3G15980 28.395 162 110 4 78 238 73 229 5.22e-12 66.6
MsG0280006894.01.T01 AT3G15980 28.395 162 110 4 78 238 73 229 5.22e-12 66.6
MsG0280006894.01.T01 AT3G15980 28.395 162 110 4 78 238 73 229 5.22e-12 66.6
MsG0280006894.01.T01 AT3G15980 28.395 162 110 4 78 238 73 229 5.66e-12 66.6
MsG0280006894.01.T01 AT3G15980 28.395 162 110 4 78 238 73 229 5.87e-12 66.6
MsG0280006894.01.T01 AT1G52360 27.160 162 112 4 78 238 73 229 2.46e-11 64.7
MsG0280006894.01.T01 AT1G52360 27.160 162 112 4 78 238 73 229 2.46e-11 64.7
MsG0280006894.01.T01 AT1G52360 27.160 162 112 4 78 238 73 229 2.92e-11 64.3
MsG0280006894.01.T01 AT1G52360 27.160 162 112 4 78 238 117 273 3.15e-11 64.3

Find 83 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTGTACTGTCAGAATT+TGG 0.134510 2:+7713110 MsG0280006894.01.T01:CDS
AAATCATCTTGTACTGAATC+TGG 0.271979 2:+7712494 MsG0280006894.01.T01:five_prime_UTR
GGGACGATACCGTCAAATTA+TGG 0.291682 2:+7712981 MsG0280006894.01.T01:CDS
ATGATATGACTGTTTGTGTT+TGG 0.299620 2:+7714090 MsG0280006894.01.T01:CDS
ATTGTGTTTATTCATCTGTT+TGG 0.320363 2:+7713050 MsG0280006894.01.T01:CDS
TGATATGACTGTTTGTGTTT+GGG 0.333313 2:+7714091 MsG0280006894.01.T01:CDS
AGATTCAGTACAAGATGATT+TGG 0.352069 2:-7712492 None:intergenic
TAGGCGGCGATTTCGGTGAT+TGG 0.373569 2:-7712749 None:intergenic
TTTGAAAACTGGCATCGTTT+TGG 0.384636 2:-7712591 None:intergenic
ATCATACTGAGTTTGCTGTT+GGG 0.389414 2:+7714153 MsG0280006894.01.T01:CDS
TGTTCATGAAAGGAACGAAT+AGG 0.390721 2:-7712893 None:intergenic
GGGATGAACTTGTTTATGTT+TGG 0.393483 2:+7714222 MsG0280006894.01.T01:CDS
GGTGATTGGGAGAGTGGGGT+TGG 0.394635 2:-7712735 None:intergenic
GCTTCTTGCTAGTACTGGTT+GGG 0.412462 2:+7714202 MsG0280006894.01.T01:CDS
GATATGAGTGTTCTTGTTGA+AGG 0.426691 2:+7714179 MsG0280006894.01.T01:CDS
TGACGGAGTAACCGTTGAAT+GGG 0.426922 2:-7712616 None:intergenic
GGCTTCTTGCTAGTACTGGT+TGG 0.432245 2:+7714201 MsG0280006894.01.T01:CDS
AGAAACGAATCACGGCGAAC+AGG 0.432589 2:-7712947 None:intergenic
TTGACGGAGTAACCGTTGAA+TGG 0.442266 2:-7712617 None:intergenic
GGATTGTACTGTCAGAATTT+GGG 0.443764 2:+7713111 MsG0280006894.01.T01:CDS
GAAGGGCTTCTTGCTAGTAC+TGG 0.444363 2:+7714197 MsG0280006894.01.T01:CDS
ATTTCGGTGATTGGGAGAGT+GGG 0.454712 2:-7712740 None:intergenic
GATTGGGAGAGTGGGGTTGG+AGG 0.460624 2:-7712732 None:intergenic
CTTGTTTATGTTTGGCAACA+TGG 0.461447 2:+7714230 MsG0280006894.01.T01:CDS
GGTGTCGTAGGCGGCGATTT+CGG 0.462174 2:-7712756 None:intergenic
GTTCATGAAAGGAACGAATA+GGG 0.469484 2:-7712892 None:intergenic
GTAGAAGGGGCTGAATTTGA+CGG 0.470246 2:-7712633 None:intergenic
TAGGTGGAAGAGTGAGTGTT+AGG 0.471925 2:-7712567 None:intergenic
CATCATACTGAGTTTGCTGT+TGG 0.478915 2:+7714152 MsG0280006894.01.T01:CDS
AGGCGGCGATTTCGGTGATT+GGG 0.479284 2:-7712748 None:intergenic
TGGAGGAGAGTTCGAGGACG+TGG 0.479460 2:-7712715 None:intergenic
GGCAACGGCGGCGACGGTAA+TGG 0.482055 2:-7712822 None:intergenic
ACCGTCGCCGCCGTTGCCGA+TGG 0.484087 2:+7712827 MsG0280006894.01.T01:CDS
ATTTGGGATGTTCGTGAACC+AGG 0.485985 2:+7713127 MsG0280006894.01.T01:CDS
TCATACTGAGTTTGCTGTTG+GGG 0.495869 2:+7714154 MsG0280006894.01.T01:CDS
CTGATCCTAGGCATCTTGAA+AGG 0.500169 2:+7714255 MsG0280006894.01.T01:CDS
TGGGGTTGGAGGAGAGTTCG+AGG 0.501933 2:-7712721 None:intergenic
AATCATCTTGTACTGAATCT+GGG 0.505519 2:+7712495 MsG0280006894.01.T01:five_prime_UTR
CCTTCCCTTTCAAGATGCCT+AGG 0.514861 2:-7714260 None:intergenic
ATATGAGTGTTCTTGTTGAA+GGG 0.514929 2:+7714180 MsG0280006894.01.T01:CDS
AGGTTCTACAATGATTCTTC+CGG 0.515402 2:+7713869 MsG0280006894.01.T01:intron
GCCGCATGTGAGGAATTTGT+TGG 0.515565 2:+7714055 MsG0280006894.01.T01:CDS
GATGCCGTCGGCGGTGTCGT+AGG 0.517224 2:-7712768 None:intergenic
TTGAACGTTCTCACCGAAGT+GGG 0.523846 2:-7713016 None:intergenic
GATTTCGGTGATTGGGAGAG+TGG 0.524323 2:-7712741 None:intergenic
ACCATCGGCAACGGCGGCGA+CGG 0.530414 2:-7712828 None:intergenic
GAAATCTCATGTTCATGTGC+CGG 0.535517 2:-7713888 None:intergenic
GTCGCCACCGCTCAAAACTT+CGG 0.541735 2:+7712671 MsG0280006894.01.T01:CDS
ATCCACGGTCCATAATTTGA+CGG 0.542435 2:-7712990 None:intergenic
AACTTCGGCATCCTCGGCAA+CGG 0.545359 2:+7712686 MsG0280006894.01.T01:CDS
TGATCCTAGGCATCTTGAAA+GGG 0.551193 2:+7714256 MsG0280006894.01.T01:CDS
TTCGTTTCTCTCCGCTGCGT+GGG 0.552872 2:+7712961 MsG0280006894.01.T01:CDS
GCTCAAAACTTCGGCATCCT+CGG 0.554172 2:+7712680 MsG0280006894.01.T01:CDS
AGCGAGGTCGTAGAGTTTGA+CGG 0.554977 2:-7712852 None:intergenic
GTAGAGTTTGACGGAACCAT+CGG 0.556953 2:-7712843 None:intergenic
CGAATAGGGTTTGAAGTAGG+TGG 0.559454 2:-7712878 None:intergenic
ATTCGTTTCTCTCCGCTGCG+TGG 0.561476 2:+7712960 MsG0280006894.01.T01:CDS
CACCGAAGTGGGACGATCCA+CGG 0.563775 2:-7713005 None:intergenic
CGTGGAGACGGCCGTTGCCG+AGG 0.577422 2:-7712697 None:intergenic
GACGGAACCATCGGCAACGG+CGG 0.578656 2:-7712834 None:intergenic
AGAGTTCGAGGACGTGGAGA+CGG 0.581734 2:-7712709 None:intergenic
GAGCGGTGGCGACGGCGATG+CGG 0.585400 2:-7712661 None:intergenic
GCCGCCTACGACACCGCCGA+CGG 0.585566 2:+7712764 MsG0280006894.01.T01:CDS
GGCAACATGGAACTGATCCT+AGG 0.588092 2:+7714243 MsG0280006894.01.T01:CDS
ATCATCTTGTACTGAATCTG+GGG 0.588651 2:+7712496 MsG0280006894.01.T01:five_prime_UTR
GCCGTCGGCGGTGTCGTAGG+CGG 0.589550 2:-7712765 None:intergenic
TTCACGTGTATGTTCATGAA+AGG 0.590355 2:-7712903 None:intergenic
GACCGTGGATCGTCCCACTT+CGG 0.593212 2:+7713003 MsG0280006894.01.T01:CDS
TTTGAACGTTCTCACCGAAG+TGG 0.595208 2:-7713017 None:intergenic
TTTCGGTGATTGGGAGAGTG+GGG 0.608168 2:-7712739 None:intergenic
TTTGACGGAACCATCGGCAA+CGG 0.611477 2:-7712837 None:intergenic
GAACGAATAGGGTTTGAAGT+AGG 0.614418 2:-7712881 None:intergenic
CCTAGGCATCTTGAAAGGGA+AGG 0.633140 2:+7714260 MsG0280006894.01.T01:CDS
GACGGAGTAACCGTTGAATG+GGG 0.634585 2:-7712615 None:intergenic
ACCAACAAATTCCTCACATG+CGG 0.642131 2:-7714056 None:intergenic
GACGGTATCGTCCCACGCAG+CGG 0.642717 2:-7712972 None:intergenic
ACGGCATCTACGACGTCACA+TGG 0.642993 2:+7712783 MsG0280006894.01.T01:CDS
CAGCGGAGAGAAACGAATCA+CGG 0.652885 2:-7712955 None:intergenic
TACCGTCAAATTATGGACCG+TGG 0.654878 2:+7712988 MsG0280006894.01.T01:CDS
TTGAAGTAGGTGGAAGAGCG+AGG 0.660138 2:-7712868 None:intergenic
GACGTCGTAGATGCCGTCGG+CGG 0.671920 2:-7712777 None:intergenic
TGTGACGTCGTAGATGCCGT+CGG 0.679756 2:-7712780 None:intergenic
GCGAACAGGATTATAATCAA+CGG 0.693874 2:-7712933 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATAAATATAACCAATA+TGG - Chr2:7714652-7714671 None:intergenic 10.0%
!! GTTATATTTATAATTTAGAA+GGG + Chr2:7714657-7714676 MsG0280006894.01.T01:intron 10.0%
!!! TTCATTTATATGATTAATTT+TGG + Chr2:7714492-7714511 MsG0280006894.01.T01:intron 10.0%
!! AATTACTAGTAAACAAAAAA+TGG + Chr2:7715340-7715359 MsG0280006894.01.T01:intron 15.0%
!! CACATATATAAAAAGAAAAA+GGG - Chr2:7714315-7714334 None:intergenic 15.0%
!! GGTTATATTTATAATTTAGA+AGG + Chr2:7714656-7714675 MsG0280006894.01.T01:intron 15.0%
!! TTATTATTCAAATGCTTTAA+AGG - Chr2:7715407-7715426 None:intergenic 15.0%
!! AAAAACTGAATTTCAAAACT+CGG - Chr2:7715700-7715719 None:intergenic 20.0%
!! AAAGAATAACATAAAGAACT+AGG - Chr2:7714906-7714925 None:intergenic 20.0%
!! AATCCATTAAAGCAAAATTA+GGG + Chr2:7713385-7713404 MsG0280006894.01.T01:intron 20.0%
!! AGCATTTGAATAATAATTGA+AGG + Chr2:7715411-7715430 MsG0280006894.01.T01:intron 20.0%
!! ATTACTTAAAAACCTAGTAT+TGG - Chr2:7713807-7713826 None:intergenic 20.0%
!! ATTTGAATAATAATTGAAGG+TGG + Chr2:7715414-7715433 MsG0280006894.01.T01:intron 20.0%
!! CCACATATATAAAAAGAAAA+AGG - Chr2:7714316-7714335 None:intergenic 20.0%
!! TAAATATAACCAATATGGTT+TGG - Chr2:7714647-7714666 None:intergenic 20.0%
!! TAACAATTTGAGCATAAAAA+AGG - Chr2:7714941-7714960 None:intergenic 20.0%
!! TACTTAAATTAGTGTATGTT+TGG - Chr2:7713748-7713767 None:intergenic 20.0%
!! TATATCAAAATTGTAATACC+TGG - Chr2:7713148-7713167 None:intergenic 20.0%
!! TCAAAATTTAAACTTAGATC+TGG - Chr2:7715260-7715279 None:intergenic 20.0%
!! TTGTGTAAGTTTAAATTGTT+TGG + Chr2:7715114-7715133 MsG0280006894.01.T01:intron 20.0%
!!! AGAATTGATTTTGATATGTT+TGG - Chr2:7713628-7713647 None:intergenic 20.0%
!!! AGATCTAAGTTTAAATTTTG+AGG + Chr2:7715259-7715278 MsG0280006894.01.T01:intron 20.0%
!!! ATAGTTCAACTAACTTTTAA+GGG - Chr2:7713506-7713525 None:intergenic 20.0%
!!! CCTTTTTCTTTTTATATATG+TGG + Chr2:7714313-7714332 MsG0280006894.01.T01:intron 20.0%
!!! TATAGTTCAACTAACTTTTA+AGG - Chr2:7713507-7713526 None:intergenic 20.0%
!!! TTCTTTTTATATATGTGGTT+AGG + Chr2:7714318-7714337 MsG0280006894.01.T01:intron 20.0%
!!! TTTTAGCTATAGTTAAGTTA+AGG + Chr2:7713301-7713320 MsG0280006894.01.T01:intron 20.0%
! ATCCATTAAAGCAAAATTAG+GGG + Chr2:7713386-7713405 MsG0280006894.01.T01:intron 25.0%
! ATGAATGTATAATTGCAACT+GGG + Chr2:7713935-7713954 MsG0280006894.01.T01:intron 25.0%
! ATTGTGTTTATTCATCTGTT+TGG + Chr2:7713050-7713069 MsG0280006894.01.T01:CDS 25.0%
! ATTTATAATTTAGAAGGGAC+CGG + Chr2:7714662-7714681 MsG0280006894.01.T01:intron 25.0%
! GAATCCATTAAAGCAAAATT+AGG + Chr2:7713384-7713403 MsG0280006894.01.T01:intron 25.0%
! GTTGTTGAAACTTTCATAAT+GGG + Chr2:7714991-7715010 MsG0280006894.01.T01:intron 25.0%
! TAACTATTACATCCAATACT+AGG + Chr2:7713792-7713811 MsG0280006894.01.T01:intron 25.0%
! TAGATAAAAACAGATCTGTT+TGG + Chr2:7715597-7715616 MsG0280006894.01.T01:intron 25.0%
! TATCTATTACATCTAATACC+AGG + Chr2:7713849-7713868 MsG0280006894.01.T01:intron 25.0%
! TTAGAAGACAATGTTAATAG+AGG - Chr2:7714875-7714894 None:intergenic 25.0%
!! AATTATAAGTTTTGCCAGTA+CGG + Chr2:7715077-7715096 MsG0280006894.01.T01:intron 25.0%
!! GTTGAAATATTTTGTGTATC+TGG - Chr2:7715198-7715217 None:intergenic 25.0%
!! TCTAAGTGTATGTTTCATTT+TGG + Chr2:7713231-7713250 MsG0280006894.01.T01:intron 25.0%
!!! AAACAGATCTGTTTTTATCT+AGG - Chr2:7715598-7715617 None:intergenic 25.0%
!!! AATTTTGACTCTTCCAAAAT+TGG + Chr2:7713720-7713739 MsG0280006894.01.T01:intron 25.0%
!!! TTGGATTTTGTTTGTAACAT+GGG + Chr2:7715040-7715059 MsG0280006894.01.T01:intron 25.0%
!!! TTTTTTTTGCATTACGTTGT+TGG + Chr2:7714602-7714621 MsG0280006894.01.T01:intron 25.0%
AAATCATCTTGTACTGAATC+TGG + Chr2:7712494-7712513 MsG0280006894.01.T01:five_prime_UTR 30.0%
AATACATCTCTTTCATTGAC+TGG + Chr2:7714414-7714433 MsG0280006894.01.T01:intron 30.0%
AATCATCTTGTACTGAATCT+GGG + Chr2:7712495-7712514 MsG0280006894.01.T01:five_prime_UTR 30.0%
AGAACTTTAGATGACCTAAA+AGG + Chr2:7714961-7714980 MsG0280006894.01.T01:intron 30.0%
ATATGAGTGTTCTTGTTGAA+GGG + Chr2:7714180-7714199 MsG0280006894.01.T01:CDS 30.0%
ATGATATGACTGTTTGTGTT+TGG + Chr2:7714090-7714109 MsG0280006894.01.T01:CDS 30.0%
CATTGATACAACAAGACTAT+TGG - Chr2:7714366-7714385 None:intergenic 30.0%
CTGATACTATACAAATCTCA+AGG - Chr2:7714773-7714792 None:intergenic 30.0%
GATGAATGTATAATTGCAAC+TGG + Chr2:7713934-7713953 MsG0280006894.01.T01:intron 30.0%
GGTTGTTGAAACTTTCATAA+TGG + Chr2:7714990-7715009 MsG0280006894.01.T01:intron 30.0%
TGATAAGTCAGTTAAGGTTT+GGG + Chr2:7713963-7713982 MsG0280006894.01.T01:intron 30.0%
TGATATGACTGTTTGTGTTT+GGG + Chr2:7714091-7714110 MsG0280006894.01.T01:CDS 30.0%
TGTAAAAATGAGCTGAACAT+CGG + Chr2:7713342-7713361 MsG0280006894.01.T01:intron 30.0%
TTGATAAGTCAGTTAAGGTT+TGG + Chr2:7713962-7713981 MsG0280006894.01.T01:intron 30.0%
TTTATAATTTAGAAGGGACC+GGG + Chr2:7714663-7714682 MsG0280006894.01.T01:intron 30.0%
! CACGATTTTAAGCAACAAAA+TGG - Chr2:7714521-7714540 None:intergenic 30.0%
! GATTTTCTCTGATCTGTTAT+GGG + Chr2:7715315-7715334 MsG0280006894.01.T01:intron 30.0%
! TACCCCTAATTTTGCTTTAA+TGG - Chr2:7713391-7713410 None:intergenic 30.0%
! TGATTTTCTCTGATCTGTTA+TGG + Chr2:7715314-7715333 MsG0280006894.01.T01:intron 30.0%
!! ATACGGGTTAGAATTGATTA+TGG - Chr2:7713657-7713676 None:intergenic 30.0%
!! GTTGGATTTTGTTTGTAACA+TGG + Chr2:7715039-7715058 MsG0280006894.01.T01:intron 30.0%
!! TGTATGTTTGGTTCCAATTT+TGG - Chr2:7713736-7713755 None:intergenic 30.0%
!! TGTTTGTGTTTGGGATTTTA+TGG + Chr2:7714100-7714119 MsG0280006894.01.T01:CDS 30.0%
AATGCACTAAAATGGAAACC+CGG - Chr2:7714684-7714703 None:intergenic 35.0%
AATTCTAACCCGTATAGAAG+TGG + Chr2:7713662-7713681 MsG0280006894.01.T01:intron 35.0%
ACATCTCATCTAAACTCTGA+TGG - Chr2:7715573-7715592 None:intergenic 35.0%
ACTAGGCTGTCAATAGTTTA+AGG + Chr2:7715378-7715397 MsG0280006894.01.T01:intron 35.0%
AGATGACCTAAAAGGAAGAA+TGG + Chr2:7714969-7714988 MsG0280006894.01.T01:intron 35.0%
AGGTTCTACAATGATTCTTC+CGG + Chr2:7713869-7713888 MsG0280006894.01.T01:intron 35.0%
ATATATGTGGTTAGGATGAG+AGG + Chr2:7714326-7714345 MsG0280006894.01.T01:intron 35.0%
ATCATCTTGTACTGAATCTG+GGG + Chr2:7712496-7712515 MsG0280006894.01.T01:five_prime_UTR 35.0%
ATGTATAATTGCAACTGGGT+CGG + Chr2:7713939-7713958 MsG0280006894.01.T01:intron 35.0%
CCTCAGATAATGCACTAAAA+TGG - Chr2:7714692-7714711 None:intergenic 35.0%
CTTGTTTATGTTTGGCAACA+TGG + Chr2:7714230-7714249 MsG0280006894.01.T01:CDS 35.0%
GATATGAGTGTTCTTGTTGA+AGG + Chr2:7714179-7714198 MsG0280006894.01.T01:CDS 35.0%
GCGAACAGGATTATAATCAA+CGG - Chr2:7712936-7712955 None:intergenic 35.0%
GCTTCAAGCAGAATCAATTA+TGG + Chr2:7713574-7713593 MsG0280006894.01.T01:intron 35.0%
GGATTGTACTGTCAGAATTT+GGG + Chr2:7713111-7713130 MsG0280006894.01.T01:CDS 35.0%
GGGTTAGAATTGATTATGGA+GGG - Chr2:7713653-7713672 None:intergenic 35.0%
GTGTAGAATCAGTGTATGTT+TGG - Chr2:7713690-7713709 None:intergenic 35.0%
GTTCATGAAAGGAACGAATA+GGG - Chr2:7712895-7712914 None:intergenic 35.0%
GTTTGGAAGACAGAAGTAAT+GGG - Chr2:7714630-7714649 None:intergenic 35.0%
TCAAGCAGAATCAATTATGG+AGG + Chr2:7713577-7713596 MsG0280006894.01.T01:intron 35.0%
TGTTCATGAAAGGAACGAAT+AGG - Chr2:7712896-7712915 None:intergenic 35.0%
TTAATAGAGGCAGAAACAGT+TGG - Chr2:7714862-7714881 None:intergenic 35.0%
TTCACGTGTATGTTCATGAA+AGG - Chr2:7712906-7712925 None:intergenic 35.0%
TTCTGTCTTCCAAACCATAT+TGG + Chr2:7714635-7714654 MsG0280006894.01.T01:intron 35.0%
TTTGGAAGACAGAAGTAATG+GGG - Chr2:7714629-7714648 None:intergenic 35.0%
! CAACAACCATTCTTCCTTTT+AGG - Chr2:7714978-7714997 None:intergenic 35.0%
! GGGATGAACTTGTTTATGTT+TGG + Chr2:7714222-7714241 MsG0280006894.01.T01:CDS 35.0%
! GTCTTGTTGTATCAATGTTG+AGG + Chr2:7714369-7714388 MsG0280006894.01.T01:intron 35.0%
! GTTTTCAAAACCCCATTCAA+CGG + Chr2:7712605-7712624 MsG0280006894.01.T01:CDS 35.0%
! TGTGACTAAAAGTGAGTTGA+AGG + Chr2:7713454-7713473 MsG0280006894.01.T01:intron 35.0%
! TGTTTGGTTCCACTTCTATA+CGG - Chr2:7713674-7713693 None:intergenic 35.0%
! TTTGAAAACTGGCATCGTTT+TGG - Chr2:7712594-7712613 None:intergenic 35.0%
!! ATCATACTGAGTTTGCTGTT+GGG + Chr2:7714153-7714172 MsG0280006894.01.T01:CDS 35.0%
!! CCATTTTAGTGCATTATCTG+AGG + Chr2:7714689-7714708 MsG0280006894.01.T01:intron 35.0%
!! TTGTGTTTGGGATTTTATGG+TGG + Chr2:7714103-7714122 MsG0280006894.01.T01:CDS 35.0%
!! TTTTATGGTGGAAGATGCTT+TGG + Chr2:7714115-7714134 MsG0280006894.01.T01:CDS 35.0%
!!! AAGTTGGTTTTTCACAAAGC+GGG - Chr2:7715652-7715671 None:intergenic 35.0%
!!! GAAGTTGGTTTTTCACAAAG+CGG - Chr2:7715653-7715672 None:intergenic 35.0%
!!! TTGAATGGGGTTTTGAAAAC+TGG - Chr2:7712605-7712624 None:intergenic 35.0%
ACCAACAAATTCCTCACATG+CGG - Chr2:7714059-7714078 None:intergenic 40.0%
ACTGTTTACCTTCTTGACTG+GGG + Chr2:7714806-7714825 MsG0280006894.01.T01:intron 40.0%
AGAAGCCGATGCAAAAACAT+CGG - Chr2:7713089-7713108 None:intergenic 40.0%
ATAACCATGCCCATTAAGCA+CGG - Chr2:7714013-7714032 None:intergenic 40.0%
ATCCACGGTCCATAATTTGA+CGG - Chr2:7712993-7713012 None:intergenic 40.0%
CAAGTGCAAGACAATGAAGT+TGG + Chr2:7715021-7715040 MsG0280006894.01.T01:intron 40.0%
CACTGTTTACCTTCTTGACT+GGG + Chr2:7714805-7714824 MsG0280006894.01.T01:intron 40.0%
GAAATCTCATGTTCATGTGC+CGG - Chr2:7713891-7713910 None:intergenic 40.0%
GCATGGTTATGCTGTTAGAA+AGG + Chr2:7714023-7714042 MsG0280006894.01.T01:intron 40.0%
GGAAGAATCATTGTAGAACC+TGG - Chr2:7713870-7713889 None:intergenic 40.0%
GGATAAGGAGTTATAGGTTC+CGG + Chr2:7713984-7714003 MsG0280006894.01.T01:intron 40.0%
GGGATTGTACTGTCAGAATT+TGG + Chr2:7713110-7713129 MsG0280006894.01.T01:CDS 40.0%
GGTTAGGATGAGAGGAATAT+TGG + Chr2:7714334-7714353 MsG0280006894.01.T01:intron 40.0%
GGTTGTGACTTACAACAACT+AGG + Chr2:7715361-7715380 MsG0280006894.01.T01:intron 40.0%
GGTTTGGAAGACAGAAGTAA+TGG - Chr2:7714631-7714650 None:intergenic 40.0%
TCACTGTTTACCTTCTTGAC+TGG + Chr2:7714804-7714823 MsG0280006894.01.T01:intron 40.0%
TGAGATTTCTTTCGTGCGAT+TGG + Chr2:7713902-7713921 MsG0280006894.01.T01:intron 40.0%
TGATCCTAGGCATCTTGAAA+GGG + Chr2:7714256-7714275 MsG0280006894.01.T01:CDS 40.0%
! AATAGTAGAGCATTCCGTAC+TGG - Chr2:7715094-7715113 None:intergenic 40.0%
! CGGGTTAGAATTGATTATGG+AGG - Chr2:7713654-7713673 None:intergenic 40.0%
! GAACGAATAGGGTTTGAAGT+AGG - Chr2:7712884-7712903 None:intergenic 40.0%
! GGTTTGGGATAAGGAGTTAT+AGG + Chr2:7713978-7713997 MsG0280006894.01.T01:intron 40.0%
! GTCAGTTAAGGTTTGGGATA+AGG + Chr2:7713969-7713988 MsG0280006894.01.T01:intron 40.0%
! GTTTGGTTCCACTTCTATAC+GGG - Chr2:7713673-7713692 None:intergenic 40.0%
! TAGGGGTATGTTTGATTGTG+CGG + Chr2:7713403-7713422 MsG0280006894.01.T01:intron 40.0%
! TCTGAGGAAAATTTTCAGCG+TGG + Chr2:7714705-7714724 MsG0280006894.01.T01:intron 40.0%
! TGCTCTGACAGTGAGTATTT+TGG + Chr2:7715226-7715245 MsG0280006894.01.T01:intron 40.0%
! TTCCTAGTTCTTGTATGCGT+AGG + Chr2:7714747-7714766 MsG0280006894.01.T01:intron 40.0%
!! AGGGAAGGTACTCAAAACTT+AGG + Chr2:7714275-7714294 MsG0280006894.01.T01:intron 40.0%
!! CATCATACTGAGTTTGCTGT+TGG + Chr2:7714152-7714171 MsG0280006894.01.T01:CDS 40.0%
!! GTCGGTTGATAAGTCAGTTA+AGG + Chr2:7713957-7713976 MsG0280006894.01.T01:intron 40.0%
!! TCATACTGAGTTTGCTGTTG+GGG + Chr2:7714154-7714173 MsG0280006894.01.T01:CDS 40.0%
!! TCTGACAGTGAGTATTTTGG+AGG + Chr2:7715229-7715248 MsG0280006894.01.T01:intron 40.0%
!!! AAACTGGCATCGTTTTGGTT+AGG - Chr2:7712589-7712608 None:intergenic 40.0%
!!! ATGTTTTTGCATCGGCTTCT+GGG + Chr2:7713089-7713108 MsG0280006894.01.T01:CDS 40.0%
ACTCGCTACGTTCAACTGTA+AGG - Chr2:7715676-7715695 None:intergenic 45.0%
ATTTCGGTGATTGGGAGAGT+GGG - Chr2:7712743-7712762 None:intergenic 45.0%
CGTTCAACTGTAAGGGAAGT+TGG - Chr2:7715668-7715687 None:intergenic 45.0%
CTCGCTACGTTCAACTGTAA+GGG - Chr2:7715675-7715694 None:intergenic 45.0%
CTGATCCTAGGCATCTTGAA+AGG + Chr2:7714255-7714274 MsG0280006894.01.T01:CDS 45.0%
GGGACGATACCGTCAAATTA+TGG + Chr2:7712981-7713000 MsG0280006894.01.T01:CDS 45.0%
GTAGAAGGGGCTGAATTTGA+CGG - Chr2:7712636-7712655 None:intergenic 45.0%
GTAGAGTTTGACGGAACCAT+CGG - Chr2:7712846-7712865 None:intergenic 45.0%
TACCGTCAAATTATGGACCG+TGG + Chr2:7712988-7713007 MsG0280006894.01.T01:CDS 45.0%
TAGGTGGAAGAGTGAGTGTT+AGG - Chr2:7712570-7712589 None:intergenic 45.0%
TGACGGAGTAACCGTTGAAT+GGG - Chr2:7712619-7712638 None:intergenic 45.0%
TTGAACGTTCTCACCGAAGT+GGG - Chr2:7713019-7713038 None:intergenic 45.0%
TTGACGGAGTAACCGTTGAA+TGG - Chr2:7712620-7712639 None:intergenic 45.0%
TTTGAACGTTCTCACCGAAG+TGG - Chr2:7713020-7713039 None:intergenic 45.0%
! ATTTGGGATGTTCGTGAACC+AGG + Chr2:7713127-7713146 MsG0280006894.01.T01:CDS 45.0%
! CGAATAGGGTTTGAAGTAGG+TGG - Chr2:7712881-7712900 None:intergenic 45.0%
! GTAAATTTTCGCCGCATGTG+AGG + Chr2:7714045-7714064 MsG0280006894.01.T01:CDS 45.0%
!! GCTTCTTGCTAGTACTGGTT+GGG + Chr2:7714202-7714221 MsG0280006894.01.T01:CDS 45.0%
!! TCACGCCGATGTTTTTGCAT+CGG + Chr2:7713081-7713100 MsG0280006894.01.T01:CDS 45.0%
!!! GATGTTTTTGCATCGGCTTC+TGG + Chr2:7713088-7713107 MsG0280006894.01.T01:CDS 45.0%
!!! TGTTTTTGCATCGGCTTCTG+GGG + Chr2:7713090-7713109 MsG0280006894.01.T01:CDS 45.0%
AGAAACGAATCACGGCGAAC+AGG - Chr2:7712950-7712969 None:intergenic 50.0%
AGCGAGGTCGTAGAGTTTGA+CGG - Chr2:7712855-7712874 None:intergenic 50.0%
CAGCGGAGAGAAACGAATCA+CGG - Chr2:7712958-7712977 None:intergenic 50.0%
CCTAGGCATCTTGAAAGGGA+AGG + Chr2:7714260-7714279 MsG0280006894.01.T01:CDS 50.0%
CCTTCCCTTTCAAGATGCCT+AGG - Chr2:7714263-7714282 None:intergenic 50.0%
GAAGGGCTTCTTGCTAGTAC+TGG + Chr2:7714197-7714216 MsG0280006894.01.T01:CDS 50.0%
GACGGAGTAACCGTTGAATG+GGG - Chr2:7712618-7712637 None:intergenic 50.0%
GATTTCGGTGATTGGGAGAG+TGG - Chr2:7712744-7712763 None:intergenic 50.0%
GCCGCATGTGAGGAATTTGT+TGG + Chr2:7714055-7714074 MsG0280006894.01.T01:CDS 50.0%
GCTCAAAACTTCGGCATCCT+CGG + Chr2:7712680-7712699 MsG0280006894.01.T01:CDS 50.0%
GGCAACATGGAACTGATCCT+AGG + Chr2:7714243-7714262 MsG0280006894.01.T01:CDS 50.0%
GGCCTACGCATACAAGAACT+AGG - Chr2:7714752-7714771 None:intergenic 50.0%
TTCCGGTTGCCGTGCTTAAT+GGG + Chr2:7714001-7714020 MsG0280006894.01.T01:intron 50.0%
TTGAAGTAGGTGGAAGAGCG+AGG - Chr2:7712871-7712890 None:intergenic 50.0%
TTTACCTTCTTGACTGGGGC+TGG + Chr2:7714810-7714829 MsG0280006894.01.T01:intron 50.0%
TTTCGGTGATTGGGAGAGTG+GGG - Chr2:7712742-7712761 None:intergenic 50.0%
TTTGACGGAACCATCGGCAA+CGG - Chr2:7712840-7712859 None:intergenic 50.0%
! CGATGCGGTTTTCGTAGAAG+GGG - Chr2:7712649-7712668 None:intergenic 50.0%
! GCGATGCGGTTTTCGTAGAA+GGG - Chr2:7712650-7712669 None:intergenic 50.0%
!! GGCTTCTTGCTAGTACTGGT+TGG + Chr2:7714201-7714220 MsG0280006894.01.T01:CDS 50.0%
!!! CTGGCATCGTTTTGGTTAGG+TGG - Chr2:7712586-7712605 None:intergenic 50.0%
!!! GAGGATGCCGAAGTTTTGAG+CGG - Chr2:7712681-7712700 None:intergenic 50.0%
AACTTCGGCATCCTCGGCAA+CGG + Chr2:7712686-7712705 MsG0280006894.01.T01:CDS 55.0%
ACGGCATCTACGACGTCACA+TGG + Chr2:7712783-7712802 MsG0280006894.01.T01:CDS 55.0%
AGAGTTCGAGGACGTGGAGA+CGG - Chr2:7712712-7712731 None:intergenic 55.0%
AGGCGGCGATTTCGGTGATT+GGG - Chr2:7712751-7712770 None:intergenic 55.0%
ATTCGTTTCTCTCCGCTGCG+TGG + Chr2:7712960-7712979 MsG0280006894.01.T01:CDS 55.0%
CAATCCAGCCCCAGTCAAGA+AGG - Chr2:7714817-7714836 None:intergenic 55.0%
GCAAAAACATCGGCGTGACG+AGG - Chr2:7713079-7713098 None:intergenic 55.0%
GTCGCCACCGCTCAAAACTT+CGG + Chr2:7712671-7712690 MsG0280006894.01.T01:CDS 55.0%
GTTCCGGTTGCCGTGCTTAA+TGG + Chr2:7714000-7714019 MsG0280006894.01.T01:intron 55.0%
TAGGCGGCGATTTCGGTGAT+TGG - Chr2:7712752-7712771 None:intergenic 55.0%
TGCCCATTAAGCACGGCAAC+CGG - Chr2:7714006-7714025 None:intergenic 55.0%
TGGGGCTGGATTGCTGTACT+TGG + Chr2:7714824-7714843 MsG0280006894.01.T01:intron 55.0%
TGTGACGTCGTAGATGCCGT+CGG - Chr2:7712783-7712802 None:intergenic 55.0%
TTCGTTTCTCTCCGCTGCGT+GGG + Chr2:7712961-7712980 MsG0280006894.01.T01:CDS 55.0%
! GGCGATGCGGTTTTCGTAGA+AGG - Chr2:7712651-7712670 None:intergenic 55.0%
! GTTGCCGTGCTTAATGGGCA+TGG + Chr2:7714006-7714025 MsG0280006894.01.T01:intron 55.0%
!!! GAAGTTTTGAGCGGTGGCGA+CGG - Chr2:7712672-7712691 None:intergenic 55.0%
!!! GATGCCGAAGTTTTGAGCGG+TGG - Chr2:7712678-7712697 None:intergenic 55.0%
CACCGAAGTGGGACGATCCA+CGG - Chr2:7713008-7713027 None:intergenic 60.0%
GACCGTGGATCGTCCCACTT+CGG + Chr2:7713003-7713022 MsG0280006894.01.T01:CDS 60.0%
GATTGGGAGAGTGGGGTTGG+AGG - Chr2:7712735-7712754 None:intergenic 60.0%
GGTGATTGGGAGAGTGGGGT+TGG - Chr2:7712738-7712757 None:intergenic 60.0%
TGGAGGAGAGTTCGAGGACG+TGG - Chr2:7712718-7712737 None:intergenic 60.0%
TGGGGTTGGAGGAGAGTTCG+AGG - Chr2:7712724-7712743 None:intergenic 60.0%
! GGTGTCGTAGGCGGCGATTT+CGG - Chr2:7712759-7712778 None:intergenic 60.0%
GACGGAACCATCGGCAACGG+CGG - Chr2:7712837-7712856 None:intergenic 65.0%
GACGGTATCGTCCCACGCAG+CGG - Chr2:7712975-7712994 None:intergenic 65.0%
GACGTCGTAGATGCCGTCGG+CGG - Chr2:7712780-7712799 None:intergenic 65.0%
ACCATCGGCAACGGCGGCGA+CGG - Chr2:7712831-7712850 None:intergenic 70.0%
GGCAACGGCGGCGACGGTAA+TGG - Chr2:7712825-7712844 None:intergenic 70.0%
!! GATGCCGTCGGCGGTGTCGT+AGG - Chr2:7712771-7712790 None:intergenic 70.0%
ACCGTCGCCGCCGTTGCCGA+TGG + Chr2:7712827-7712846 MsG0280006894.01.T01:CDS 75.0%
CGTGGAGACGGCCGTTGCCG+AGG - Chr2:7712700-7712719 None:intergenic 75.0%
GAGCGGTGGCGACGGCGATG+CGG - Chr2:7712664-7712683 None:intergenic 75.0%
GCCGCCTACGACACCGCCGA+CGG + Chr2:7712764-7712783 MsG0280006894.01.T01:CDS 75.0%
!! GCCGTCGGCGGTGTCGTAGG+CGG - Chr2:7712768-7712787 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 7712494 7715721 7712494 ID=MsG0280006894.01;Name=MsG0280006894.01
Chr2 mRNA 7712494 7715721 7712494 ID=MsG0280006894.01.T01;Parent=MsG0280006894.01;Name=MsG0280006894.01.T01;_AED=0.31;_eAED=0.31;_QI=105|0.33|0.25|0.75|1|1|4|0|291
Chr2 exon 7712494 7713148 7712494 ID=MsG0280006894.01.T01:exon:7924;Parent=MsG0280006894.01.T01
Chr2 exon 7713871 7713914 7713871 ID=MsG0280006894.01.T01:exon:7925;Parent=MsG0280006894.01.T01
Chr2 exon 7714041 7714281 7714041 ID=MsG0280006894.01.T01:exon:7926;Parent=MsG0280006894.01.T01
Chr2 exon 7715681 7715721 7715681 ID=MsG0280006894.01.T01:exon:7927;Parent=MsG0280006894.01.T01
Chr2 five_prime_UTR 7712494 7712598 7712494 ID=MsG0280006894.01.T01:five_prime_utr;Parent=MsG0280006894.01.T01
Chr2 CDS 7712599 7713148 7712599 ID=MsG0280006894.01.T01:cds;Parent=MsG0280006894.01.T01
Chr2 CDS 7713871 7713914 7713871 ID=MsG0280006894.01.T01:cds;Parent=MsG0280006894.01.T01
Chr2 CDS 7714041 7714281 7714041 ID=MsG0280006894.01.T01:cds;Parent=MsG0280006894.01.T01
Chr2 CDS 7715681 7715721 7715681 ID=MsG0280006894.01.T01:cds;Parent=MsG0280006894.01.T01
Gene Sequence

>MsG0280006894.01.T01

ATGCCAGTTTTCAAAACCCCATTCAACGGTTACTCCGTCAAATTCAGCCCCTTCTACGAAAACCGCATCGCCGTCGCCACCGCTCAAAACTTCGGCATCCTCGGCAACGGCCGTCTCCACGTCCTCGAACTCTCCTCCAACCCCACTCTCCCAATCACCGAAATCGCCGCCTACGACACCGCCGACGGCATCTACGACGTCACATGGTCTGAATCTCACGACTCCATTACCGTCGCCGCCGTTGCCGATGGTTCCGTCAAACTCTACGACCTCGCTCTTCCACCTACTTCAAACCCTATTCGTTCCTTTCATGAACATACACGTGAAGTTCATTCCGTTGATTATAATCCTGTTCGCCGTGATTCGTTTCTCTCCGCTGCGTGGGACGATACCGTCAAATTATGGACCGTGGATCGTCCCACTTCGGTGAGAACGTTCAAAGAGCATGCTTATTGTGTTTATTCATCTGTTTGGAATCCTCGTCACGCCGATGTTTTTGCATCGGCTTCTGGGGATTGTACTGTCAGAATTTGGGATGTTCGTGAACCAGGTTCTACAATGATTCTTCCGGCACATGAACATGAGATTTCTTTCAAAGGTAAATTTTCGCCGCATGTGAGGAATTTGTTGGTGTCTTGTTCTTATGATATGACTGTTTGTGTTTGGGATTTTATGGTGGAAGATGCTTTGGTGAGTAGATATGATCATCATACTGAGTTTGCTGTTGGGGTTGATATGAGTGTTCTTGTTGAAGGGCTTCTTGCTAGTACTGGTTGGGATGAACTTGTTTATGTTTGGCAACATGGAACTGATCCTAGGCATCTTGAAAGGGAAGTTGAACGTAGCGAGTTCCGAGTTTTGAAATTCAGTTTTTAG
Protein sequence

>MsG0280006894.01.T01

MPVFKTPFNGYSVKFSPFYENRIAVATAQNFGILGNGRLHVLELSSNPTLPITEIAAYDTADGIYDVTWSESHDSITVAAVADGSVKLYDLALPPTSNPIRSFHEHTREVHSVDYNPVRRDSFLSAAWDDTVKLWTVDRPTSVRTFKEHAYCVYSSVWNPRHADVFASASGDCTVRIWDVREPGSTMILPAHEHEISFKGKFSPHVRNLLVSCSYDMTVCVWDFMVEDALVSRYDHHTEFAVGVDMSVLVEGLLASTGWDELVYVWQHGTDPRHLEREVERSEFRVLKFSF*