AlfalfaGEDB | MsG0280006925.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006925.01

KEGG: K08266
GO: GO:0005515 GO:0016020 GO:0016021
PFAM: PF00400 PF00892

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006925.01.T01	KAG4945701.1	59.72	787	242	12	174	948	6	729	0	853

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006925.01.T01	Q9AYE4	72.703	370	47	2	579	948	1	316	0	558

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006925.01.T01	A0A072V467	93.258	356	3	1	170	504	1	356	0.0	660

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280006925.01	MsG0280007203.01	0.811243	7.718877e-51	4.753403e-48

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006925.01.T01	MTR_2g016710	93.258	356	3	1	170	504	1	356	0.0	660
MsG0280006925.01.T01	MTR_2g016690	79.481	385	25	4	564	948	1	331	0.0	607
MsG0280006925.01.T01	MTR_2g016710	90.064	312	10	1	170	460	1	312	0.0	554
MsG0280006925.01.T01	MTR_1g114210	50.000	344	145	4	172	490	5	346	7.88e-112	348
MsG0280006925.01.T01	MTR_1g038560	49.558	339	145	4	176	491	2	337	2.59e-109	341
MsG0280006925.01.T01	MTR_2g007990	37.309	327	177	3	177	476	15	340	7.88e-71	240
MsG0280006925.01.T01	MTR_2g007260	36.827	353	190	5	177	497	14	365	3.11e-68	234
MsG0280006925.01.T01	MTR_2g007960	34.426	366	197	6	177	510	12	366	2.90e-65	225
MsG0280006925.01.T01	MTR_7g111830	36.446	332	181	4	178	479	8	339	3.78e-63	219
MsG0280006925.01.T01	MTR_4g094360	37.048	332	183	3	169	474	6	337	5.71e-63	219

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006925.01.T01	AT3G18140	72.727	363	41	3	579	941	1	305	0.0	539
MsG0280006925.01.T01	AT2G22040	59.384	357	87	4	584	939	11	310	6.58e-137	412
MsG0280006925.01.T01	AT3G18140	72.364	275	22	2	579	853	1	221	2.85e-134	402
MsG0280006925.01.T01	AT3G30340	48.094	341	143	4	176	492	9	339	1.33e-106	335
MsG0280006925.01.T01	AT4G01440	42.939	347	164	4	170	492	1	337	1.24e-91	295
MsG0280006925.01.T01	AT4G01440	43.279	305	149	3	170	450	1	305	4.73e-83	271
MsG0280006925.01.T01	AT1G01070	37.853	354	192	4	168	493	3	356	5.64e-74	248
MsG0280006925.01.T01	AT4G01430	36.752	351	195	4	170	493	1	351	3.48e-72	243
MsG0280006925.01.T01	AT1G11450	36.337	344	196	3	170	490	8	351	2.01e-70	238
MsG0280006925.01.T01	AT1G11460	36.778	329	184	3	170	474	8	336	1.10e-68	233

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Find 230 sgRNAs with CRISPR-Local

Find 765 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGCAATATCCGAGTG+TGG	0.617600	2:-8028631	MsG0280006925.01.T01:CDS
AAAATGTTTCTGATACTTGG+AGG	0.518625	2:-8038398	MsG0280006925.01.T01:CDS
AAACGCTACCTAGCTGCAGC+TGG	0.523547	2:-8029436	MsG0280006925.01.T01:CDS
AAAGGGAATCAAACCGAATC+AGG	0.378982	2:+8030382	None:intergenic
AAATAATGGATCGAAACACA+TGG	0.638092	2:-8025116	MsG0280006925.01.T01:three_prime_UTR
AAATATAAATAAGTCCCTCT+AGG	0.549751	2:+8037995	None:intergenic
AAATGAAGAGTTGTGAAGAA+TGG	0.523089	2:-8035602	MsG0280006925.01.T01:CDS
AAATGGCAATATCCGAGTGT+GGG	0.619634	2:-8028630	MsG0280006925.01.T01:CDS
AAATGGTAGCAATAGAGAGA+CGG	0.640702	2:+8035472	None:intergenic
AAATTCATGCAGTTGTGAGT+TGG	0.549311	2:-8028597	MsG0280006925.01.T01:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACTCATGGCCTATATT+AGG	-	Chr2:8027591-8027610	MsG0280006925.01.T01:intron	30.0%
AAACAATCAAGGTTAGCAAT+TGG	+	Chr2:8037573-8037592	None:intergenic	30.0%
AAAGTTACATAACTTACTGC+GGG	+	Chr2:8036158-8036177	None:intergenic	30.0%
AAATGAAGAGTTGTGAAGAA+TGG	-	Chr2:8027924-8027943	MsG0280006925.01.T01:CDS	30.0%
AAATTGAAGGAACTTCACTT+TGG	+	Chr2:8031454-8031473	None:intergenic	30.0%
AACAAAGCCACCTTAAAAAA+TGG	+	Chr2:8027135-8027154	None:intergenic	30.0%
AACTAAGCATTTAGGTAATC+CGG	-	Chr2:8035794-8035813	MsG0280006925.01.T01:intron	30.0%
AACTTCAGTTTGACTATAAG+TGG	+	Chr2:8028364-8028383	None:intergenic	30.0%
AATAACACACAAGATTTACG+TGG	-	Chr2:8031746-8031765	MsG0280006925.01.T01:intron	30.0%
AATCAGGATATTGAATAGTC+CGG	+	Chr2:8033131-8033150	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	8025070	8038478	8025070	ID=MsG0280006925.01;Name=MsG0280006925.01
Chr2	mRNA	8025070	8038478	8025070	ID=MsG0280006925.01.T01;Parent=MsG0280006925.01;Name=MsG0280006925.01.T01;_AED=0.39;_eAED=0.44;_QI=0\|0.42\|0.45\|0.95\|1\|1\|20\|213\|948
Chr2	exon	8025070	8025410	8025070	ID=MsG0280006925.01.T01:exon:2356;Parent=MsG0280006925.01.T01
Chr2	exon	8026344	8026403	8026344	ID=MsG0280006925.01.T01:exon:2355;Parent=MsG0280006925.01.T01
Chr2	exon	8026503	8026582	8026503	ID=MsG0280006925.01.T01:exon:2354;Parent=MsG0280006925.01.T01
Chr2	exon	8027854	8027948	8027854	ID=MsG0280006925.01.T01:exon:2353;Parent=MsG0280006925.01.T01
Chr2	exon	8028042	8028158	8028042	ID=MsG0280006925.01.T01:exon:2352;Parent=MsG0280006925.01.T01
Chr2	exon	8028598	8028675	8028598	ID=MsG0280006925.01.T01:exon:2351;Parent=MsG0280006925.01.T01
Chr2	exon	8029020	8029089	8029020	ID=MsG0280006925.01.T01:exon:2350;Parent=MsG0280006925.01.T01
Chr2	exon	8029189	8029301	8029189	ID=MsG0280006925.01.T01:exon:2349;Parent=MsG0280006925.01.T01
Chr2	exon	8029387	8029488	8029387	ID=MsG0280006925.01.T01:exon:2348;Parent=MsG0280006925.01.T01
Chr2	exon	8029734	8029833	8029734	ID=MsG0280006925.01.T01:exon:2347;Parent=MsG0280006925.01.T01
Chr2	exon	8029928	8029989	8029928	ID=MsG0280006925.01.T01:exon:2346;Parent=MsG0280006925.01.T01
Chr2	exon	8030396	8030604	8030396	ID=MsG0280006925.01.T01:exon:2345;Parent=MsG0280006925.01.T01
Chr2	exon	8032068	8032189	8032068	ID=MsG0280006925.01.T01:exon:2344;Parent=MsG0280006925.01.T01
Chr2	exon	8032863	8033021	8032863	ID=MsG0280006925.01.T01:exon:2343;Parent=MsG0280006925.01.T01
Chr2	exon	8033705	8033856	8033705	ID=MsG0280006925.01.T01:exon:2342;Parent=MsG0280006925.01.T01
Chr2	exon	8034177	8034570	8034177	ID=MsG0280006925.01.T01:exon:2341;Parent=MsG0280006925.01.T01
Chr2	exon	8034727	8034843	8034727	ID=MsG0280006925.01.T01:exon:2340;Parent=MsG0280006925.01.T01
Chr2	exon	8035441	8035704	8035441	ID=MsG0280006925.01.T01:exon:2339;Parent=MsG0280006925.01.T01
Chr2	exon	8037791	8038038	8037791	ID=MsG0280006925.01.T01:exon:2338;Parent=MsG0280006925.01.T01
Chr2	exon	8038302	8038478	8038302	ID=MsG0280006925.01.T01:exon:2337;Parent=MsG0280006925.01.T01
Chr2	CDS	8038302	8038478	8038302	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8037791	8038038	8037791	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8035441	8035704	8035441	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8034727	8034843	8034727	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8034177	8034570	8034177	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8033705	8033856	8033705	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8032863	8033021	8032863	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8032068	8032189	8032068	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8030396	8030604	8030396	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8029928	8029989	8029928	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8029734	8029833	8029734	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8029387	8029488	8029387	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8029189	8029301	8029189	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8029020	8029089	8029020	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8028598	8028675	8028598	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8028042	8028158	8028042	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8027854	8027948	8027854	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8026503	8026582	8026503	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8026344	8026403	8026344	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	CDS	8025283	8025410	8025283	ID=MsG0280006925.01.T01:cds;Parent=MsG0280006925.01.T01
Chr2	three_prime_UTR	8025070	8025282	8025070	ID=MsG0280006925.01.T01:three_prime_utr;Parent=MsG0280006925.01.T01

Gene Sequence

>MsG0280006925.01.T01

ATGACAGAGGATACTGTGATGGAGTGTTCTTTGCTGGTTCACAATGTTGTTTTGCAGGAAAATGTTTCTGATACTTGGAGGTGGTTGCTGGATCCAGTCCATGGATACACGGTGCGAGAGGCTTACCGCTACATTACAAGTAATGGTGTTCACGTCGATAGGAGTACGGTAGATGATTTTATAAGCATGTCGGGTTTGCCTAGAGGGACTTATTTATATTTTAGAATTATTTGGTTCGCTACTGTTTGGGCTATCTGGAAGGAAAGGAATAATCGTGCATTTCAAAATACGGTGTCTGATCCTTCTGTTTTAGCTCAAAAGGTTAAATTGACATCTTTCCTATGGATGAAATCTAATAAAGTTAATTTTTGCTTTAGCTATCATGATTGGTGGCGACATCCTCTTCATTGTATGGGTGTCCACATGTCTAATCTAAATTGCATACATATGCAAGTAGAATTGAATTTGCATTGTTGTTGCCTTGTTGAATACAATTGCATTGGTGAAATGAAGAGTTGTGAAGAATGGAAACCTTTTATGATAATGATAGCAATTGATATTTCTTTTGCTGTTGTGAATATTCTTCTCAAGAAAGTCCTTGATGAAGGAATGAACCATTTGGTTTTTATCACATACCGTCTCTCTATTGCTACCATTTTTGTAGGCTCAATTGGATACTTTAGGGAAAGAGCTTCAGTCACACAATACTTCTTCCTCCTGGGGATTCAATACACTTCTGCTACATTTGCTTGTGCTTTTGTCAACATGGTGCCTGTGGTCACTTTCCTATTGGCATTGCCATTTGGAATAGAGACTGTGAACATCAAATGCAACAGTGGTAGAGCCAAGATTTTTGGTACATTGGTGTGTATAGTTGGTGCATTGATGTTGACACTTTACAAAGGAAAGGGTCTATTTAATTTTTCTCATCATGAGTCATCAGTGAAGCTAGCAGCTACAAGGACACCAGAGAAATGGACTATTGGTGTGATAGCATTGATTTTGGGAACTCTCTTTTGGTCTTCTTGGTTCATTTTACAATCTAAAATAAGCAAAAGATATCCATGCCAGTATTCTAGCACAGCAATCATGAGCTTCTTTGGAGCTATTCAATCAGCTGTCATTTGCTTGTGCATTGACCATAACTTGTCTATATGGGTTCTCAGAGGAAAGATACAAATAATTGCTATTTTGTATGCTGGAATAATTGGATCAGGTTTGTGTTTTGTGGGAATGTCATGGTGTGTTAAGAAGAGGGGTCCAGTCTTTACAGCAGCATTTAGCCCCTTTGTTCAAATAATGGCAGCCTTGATTGATATCCCTGTCCTACATGAGCAGTTATATCTTGGAAGTGTGTTGGGGTCAATATTGGTGATGCTTGGATTATACATTCTTCTATGGGGTAAGAGCAAGGAGATGAAGAATCGTTTGATTAAGTTAGCGGAGAAAGCTGAAGAAACAAAGGAACAAGAACCTCAAATACAACACTTAACAGTGTCTTGTGAATCACGGTCAGTATCAAGCCCAATGTCGTTTGCTAAAGATACTATTTTCCAATTTAAAGTAGTAAGAAAGGGTCATCATCAAGAGGATTCTCACAGGACCAAAGTGAAGTTCCTTCAATTTAGTCATAATTCTGATGAATTCAATGAAATTAGGTTTTGTGTTGTTAGAGGTAAATGTATATTGATGATAGTCAAAAGAAAAAAAGAAGAGGAAGATTCGTGGTTTAGGATGAGTCAACCATCAGTGTTACTCGCCACTGCTAGCTATGATCACACCATTCGATTTTGGGAAGCTAAGAGTGGTCGCTGTTACCGGACTATTCAATATCCTGATTCGACACCAGACACACCTTCCATCAGAAGTTTTGATGCTACAAAGAATGAATTAGCTTTTATAAGCCGTGATTACATCTCTCGACTTGCTTCTAGGGCATCCTCACAGCATGGTCCCGTAGTGTTCCGTTTCACTAGTGGATTGGGCTTGGCTTGGTCCAGAACACAAGTGAATCGGCTGGAGATAACCCCGGATAAACGCTACCTAGCTGCAGCTGGCAATCCTCACATACGGTTGTTTGATGTTAACTCAAATAGCCCTCAACCGGTAATGAGCTATGACGGACATACCAGTAATGTAATGGCAGTTGGGTTCCAGTGTGATGGGAACTGGATGTATTCTGGTTCAGAGGACGGCACGGTTAAAATCTGGGATTTGAGGGCACCAGGTTGTCAGAGGGAATATGAAAGCCGTGCAGCAGTTAACACTGTTGTGTTGCACCCAAATCAGACTGAGCTAATATCTGGTGATCAAAATGGCAATATCCGAGTGTGGGATTTGACAGCAAATTCATGCAGTTGTGAGTTGGTGCCAGAGGTTGATACAGCTGTACGCTCTCTAACAGTGATGTGGGATGGCAGTTTGGTAGTAGCAGCAAATAATAATGGGACTTGTTATGTGTGGCGCCTGTTGCGAGGGACTCAGACAATGACAAACTTTGAGCCACTTCATAAGCTGCAAGCACACAATGGCTACATTCTCAAATGTGTTTTATCCCCTGAGTTTTGTGACCCCCACAGGTACTTGGCAACTGCATCTTCTGATCATACTGTTAAGATATGGAATGTTGATGGTTTTACACTGGAGAAGACTTTGATAGGTCATCAACGTTGGGTGTGGGACTGTGTCTTCTCAGTGGATGGTGCCTACCTTATTACAGCTTCCTCTGATTCAACTGCTAGGCTTTGGTCTACGACAACTGGTGAAGATATTAAGGTTTACCAAGGGCATCATAAATCTACAATTTGTTGTGCCCTACATGATGGGGCTGAGCCTGGAAGCTCTTGA

Protein sequence

>MsG0280006925.01.T01

MTEDTVMECSLLVHNVVLQENVSDTWRWLLDPVHGYTVREAYRYITSNGVHVDRSTVDDFISMSGLPRGTYLYFRIIWFATVWAIWKERNNRAFQNTVSDPSVLAQKVKLTSFLWMKSNKVNFCFSYHDWWRHPLHCMGVHMSNLNCIHMQVELNLHCCCLVEYNCIGEMKSCEEWKPFMIMIAIDISFAVVNILLKKVLDEGMNHLVFITYRLSIATIFVGSIGYFRERASVTQYFFLLGIQYTSATFACAFVNMVPVVTFLLALPFGIETVNIKCNSGRAKIFGTLVCIVGALMLTLYKGKGLFNFSHHESSVKLAATRTPEKWTIGVIALILGTLFWSSWFILQSKISKRYPCQYSSTAIMSFFGAIQSAVICLCIDHNLSIWVLRGKIQIIAILYAGIIGSGLCFVGMSWCVKKRGPVFTAAFSPFVQIMAALIDIPVLHEQLYLGSVLGSILVMLGLYILLWGKSKEMKNRLIKLAEKAEETKEQEPQIQHLTVSCESRSVSSPMSFAKDTIFQFKVVRKGHHQEDSHRTKVKFLQFSHNSDEFNEIRFCVVRGKCILMIVKRKKEEEDSWFRMSQPSVLLATASYDHTIRFWEAKSGRCYRTIQYPDSTPDTPSIRSFDATKNELAFISRDYISRLASRASSQHGPVVFRFTSGLGLAWSRTQVNRLEITPDKRYLAAAGNPHIRLFDVNSNSPQPVMSYDGHTSNVMAVGFQCDGNWMYSGSEDGTVKIWDLRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAANNNGTCYVWRLLRGTQTMTNFEPLHKLQAHNGYILKCVLSPEFCDPHRYLATASSDHTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDSTARLWSTTTGEDIKVYQGHHKSTICCALHDGAEPGSS*