Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007065.01.T01 | PNX81191.1 | 61.404 | 57 | 21 | 1 | 1 | 56 | 54 | 110 | 6.47E-13 | 73.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002716.01 | MsG0280007065.01 | 0.809219 | 2.108586e-50 | 1.230827e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007065.01.T01 | MTR_2g021210 | 50.000 | 64 | 32 | 0 | 2 | 65 | 103 | 166 | 1.62e-13 | 63.9 |
| MsG0280007065.01.T01 | MTR_2g021190 | 65.789 | 38 | 13 | 0 | 1 | 38 | 39 | 76 | 4.24e-12 | 60.1 |
| MsG0280007065.01.T01 | MTR_2g021155 | 51.786 | 56 | 24 | 1 | 1 | 56 | 54 | 106 | 7.93e-12 | 59.3 |
| MsG0280007065.01.T01 | MTR_2g021252 | 49.153 | 59 | 29 | 1 | 1 | 58 | 65 | 123 | 1.23e-11 | 58.5 |
| MsG0280007065.01.T01 | MTR_2g029990 | 54.902 | 51 | 22 | 1 | 1 | 50 | 69 | 119 | 1.29e-11 | 58.5 |
| MsG0280007065.01.T01 | MTR_7g067680 | 46.774 | 62 | 31 | 1 | 1 | 60 | 67 | 128 | 6.60e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCTGCGTGGCGGTGGCGG+CGG | 0.213325 | 2:-10108223 | None:intergenic |
| TGCTGCGTGGCGGTGGCGGC+GGG | 0.245197 | 2:-10108222 | None:intergenic |
| TGACACGCGAAGGTGCTTCT+TGG | 0.387340 | 2:-10108102 | None:intergenic |
| GCAGTTAGAGCATTCTTTCA+AGG | 0.402100 | 2:+10108242 | MsG0280007065.01.T01:CDS |
| CTCTAACTGCTGCGTGGCGG+TGG | 0.424634 | 2:-10108229 | None:intergenic |
| TAACTGCTGCGTGGCGGTGG+CGG | 0.469870 | 2:-10108226 | None:intergenic |
| TTCAATCGGTCTGTAAGCTA+TGG | 0.479191 | 2:+10108051 | MsG0280007065.01.T01:CDS |
| GCTGCGTGGCGGTGGCGGCG+GGG | 0.484245 | 2:-10108221 | None:intergenic |
| ATCCCTCATGCAATTTCAAT+CGG | 0.491087 | 2:+10108037 | None:intergenic |
| TTAGAGCATTCTTTCAAGGA+TGG | 0.519586 | 2:+10108246 | MsG0280007065.01.T01:CDS |
| AGCATTCTTTCAAGGATGGA+TGG | 0.532410 | 2:+10108250 | MsG0280007065.01.T01:CDS |
| ATATTCTGAAGAGGAGATGA+TGG | 0.554627 | 2:-10108133 | None:intergenic |
| CGTTGTGAAGATGGAGGAGA+GGG | 0.582352 | 2:-10108187 | None:intergenic |
| TCGTTGTGAAGATGGAGGAG+AGG | 0.644427 | 2:-10108188 | None:intergenic |
| GGCGCGTGGTTGACACGCGA+AGG | 0.647524 | 2:-10108112 | None:intergenic |
| ATGCTCTAACTGCTGCGTGG+CGG | 0.696210 | 2:-10108232 | None:intergenic |
| GAAGAGGAGATGATGGCGCG+TGG | 0.720049 | 2:-10108126 | None:intergenic |
| AGAATGCTCTAACTGCTGCG+TGG | 0.755470 | 2:-10108235 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTCTTAATATTCTGAAG+AGG | - | Chr2:10108145-10108164 | None:intergenic | 20.0% |
| ! | ATATTCTGAAGAGGAGATGA+TGG | - | Chr2:10108136-10108155 | None:intergenic | 35.0% |
| ! | TTAGAGCATTCTTTCAAGGA+TGG | + | Chr2:10108246-10108265 | MsG0280007065.01.T01:CDS | 35.0% |
| AGCATTCTTTCAAGGATGGA+TGG | + | Chr2:10108250-10108269 | MsG0280007065.01.T01:CDS | 40.0% | |
| TTCAATCGGTCTGTAAGCTA+TGG | + | Chr2:10108051-10108070 | MsG0280007065.01.T01:CDS | 40.0% | |
| ! | CACTTTTCTCGTTGTGAAGA+TGG | - | Chr2:10108199-10108218 | None:intergenic | 40.0% |
| ! | GCAGTTAGAGCATTCTTTCA+AGG | + | Chr2:10108242-10108261 | MsG0280007065.01.T01:CDS | 40.0% |
| ! | TTTTCTCGTTGTGAAGATGG+AGG | - | Chr2:10108196-10108215 | None:intergenic | 40.0% |
| AGAATGCTCTAACTGCTGCG+TGG | - | Chr2:10108238-10108257 | None:intergenic | 50.0% | |
| CGTTGTGAAGATGGAGGAGA+GGG | - | Chr2:10108190-10108209 | None:intergenic | 50.0% | |
| TCGTTGTGAAGATGGAGGAG+AGG | - | Chr2:10108191-10108210 | None:intergenic | 50.0% | |
| ATGCTCTAACTGCTGCGTGG+CGG | - | Chr2:10108235-10108254 | None:intergenic | 55.0% | |
| !! | TGACACGCGAAGGTGCTTCT+TGG | - | Chr2:10108105-10108124 | None:intergenic | 55.0% |
| ! | GAAGAGGAGATGATGGCGCG+TGG | - | Chr2:10108129-10108148 | None:intergenic | 60.0% |
| CTCTAACTGCTGCGTGGCGG+TGG | - | Chr2:10108232-10108251 | None:intergenic | 65.0% | |
| TAACTGCTGCGTGGCGGTGG+CGG | - | Chr2:10108229-10108248 | None:intergenic | 65.0% | |
| GGCGCGTGGTTGACACGCGA+AGG | - | Chr2:10108115-10108134 | None:intergenic | 70.0% | |
| !! | CTGCTGCGTGGCGGTGGCGG+CGG | - | Chr2:10108226-10108245 | None:intergenic | 80.0% |
| !! | TGCTGCGTGGCGGTGGCGGC+GGG | - | Chr2:10108225-10108244 | None:intergenic | 80.0% |
| !! | GCTGCGTGGCGGTGGCGGCG+GGG | - | Chr2:10108224-10108243 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 10108044 | 10108280 | 10108044 | ID=MsG0280007065.01;Name=MsG0280007065.01 |
| Chr2 | mRNA | 10108044 | 10108280 | 10108044 | ID=MsG0280007065.01.T01;Parent=MsG0280007065.01;Name=MsG0280007065.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|78 |
| Chr2 | exon | 10108044 | 10108280 | 10108044 | ID=MsG0280007065.01.T01:exon:2379;Parent=MsG0280007065.01.T01 |
| Chr2 | CDS | 10108044 | 10108280 | 10108044 | ID=MsG0280007065.01.T01:cds;Parent=MsG0280007065.01.T01 |
| Gene Sequence |
| Protein sequence |
