AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007119.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007119.01.T01 MTR_2g021050 81.633 196 32 3 2 195 189 382 7.27e-111 322
MsG0280007119.01.T01 MTR_2g425980 74.490 196 40 2 1 196 216 401 6.16e-94 280
MsG0280007119.01.T01 MTR_2g426020 66.829 205 43 4 1 198 217 403 2.42e-88 265
MsG0280007119.01.T01 MTR_2g028130 68.137 204 45 4 1 197 221 411 4.16e-87 262
MsG0280007119.01.T01 MTR_2g426060 70.312 192 46 3 1 192 217 397 2.71e-85 258
MsG0280007119.01.T01 MTR_2g031680 67.188 192 53 2 1 192 196 377 1.95e-83 252
MsG0280007119.01.T01 MTR_2g028110 68.020 197 42 4 1 197 206 381 2.79e-83 251
MsG0280007119.01.T01 MTR_2g426070 67.692 195 47 3 1 195 223 401 5.00e-83 251
MsG0280007119.01.T01 MTR_2g024100 68.020 197 48 4 1 190 212 400 6.11e-83 251
MsG0280007119.01.T01 MTR_2g031640 65.482 197 54 5 1 194 197 382 3.85e-80 244
MsG0280007119.01.T01 MTR_2g025420 66.667 198 51 4 1 192 224 412 1.85e-79 243
MsG0280007119.01.T01 MTR_0667s0010 64.322 199 53 3 1 192 210 397 6.35e-79 241
MsG0280007119.01.T01 MTR_2g426090 64.583 192 53 2 2 193 220 396 1.22e-76 236
MsG0280007119.01.T01 MTR_2g031720 63.265 196 58 4 1 192 199 384 3.00e-76 234
MsG0280007119.01.T01 MTR_2g025960 59.799 199 57 3 1 192 125 307 9.21e-76 230
MsG0280007119.01.T01 MTR_2g026010 64.532 203 49 5 1 192 196 386 1.31e-74 230
MsG0280007119.01.T01 MTR_8g099460 61.881 202 53 5 1 195 211 395 2.01e-74 229
MsG0280007119.01.T01 MTR_1g068875 64.583 192 50 4 2 193 198 371 2.65e-74 229
MsG0280007119.01.T01 MTR_3g083010 63.415 205 52 6 1 195 226 417 2.22e-73 227
MsG0280007119.01.T01 MTR_2g028140 62.887 194 58 3 1 192 225 406 2.98e-73 227
MsG0280007119.01.T01 MTR_1g068835 62.694 193 54 4 1 193 198 372 5.37e-72 223
MsG0280007119.01.T01 MTR_1g068860 62.944 197 53 5 1 197 200 376 8.16e-72 222
MsG0280007119.01.T01 MTR_2g028160 60.000 200 58 5 1 190 221 408 1.02e-68 216
MsG0280007119.01.T01 MTR_2g037520 65.409 159 40 2 1 152 1 151 8.41e-64 195
MsG0280007119.01.T01 MTR_4g070550 57.068 191 67 4 1 188 219 397 8.95e-61 194
MsG0280007119.01.T01 MTR_2g024110 55.556 189 58 5 1 178 197 370 1.12e-60 194
MsG0280007119.01.T01 MTR_2g025440 65.942 138 32 3 60 190 1 130 1.63e-54 173
MsG0280007119.01.T01 MTR_4g006270 47.716 197 70 5 1 178 176 358 2.46e-49 165
MsG0280007119.01.T01 MTR_4g133170 43.842 203 88 6 1 195 200 384 1.94e-41 144
MsG0280007119.01.T01 MTR_1g068905 48.223 197 36 6 2 198 182 312 1.54e-40 140
MsG0280007119.01.T01 MTR_7g110360 55.814 129 34 3 62 174 199 320 4.37e-35 126
MsG0280007119.01.T01 MTR_3g086960 48.447 161 54 6 24 173 9 151 8.99e-34 118
MsG0280007119.01.T01 MTR_7g110370 79.688 64 13 0 63 126 34 97 1.58e-29 109
MsG0280007119.01.T01 MTR_5g083940 39.869 153 55 4 1 147 153 274 2.00e-22 92.8
MsG0280007119.01.T01 MTR_2g031700 67.143 70 15 2 127 196 232 293 3.36e-20 86.3
MsG0280007119.01.T01 MTR_2g028120 57.500 80 25 3 114 192 7 78 5.33e-20 80.5
MsG0280007119.01.T01 MTR_2g040050 43.802 121 34 4 2 117 150 241 1.96e-19 83.6
MsG0280007119.01.T01 MTR_3g014570 30.114 176 95 5 2 173 229 380 4.09e-16 75.9
MsG0280007119.01.T01 MTR_3g036290 31.250 176 92 4 2 174 210 359 5.41e-15 72.4
MsG0280007119.01.T01 MTR_3g006670 30.601 183 91 7 2 172 252 410 2.77e-14 70.9
MsG0280007119.01.T01 MTR_3g024130 28.736 174 97 4 2 172 166 315 3.60e-14 70.1
MsG0280007119.01.T01 MTR_3g436150 37.302 126 67 4 4 128 244 358 4.11e-14 70.1
MsG0280007119.01.T01 MTR_2g046900 31.461 178 99 7 1 174 229 387 4.51e-14 70.1
MsG0280007119.01.T01 MTR_8g054320 31.429 175 90 7 2 172 153 301 6.83e-14 68.9
MsG0280007119.01.T01 MTR_3g014540 31.818 176 90 8 2 173 230 379 8.17e-14 69.3
MsG0280007119.01.T01 MTR_3g010390 32.022 178 93 8 2 172 242 398 1.07e-13 68.9
MsG0280007119.01.T01 MTR_2g021150 33.721 172 96 6 2 172 215 369 1.60e-13 68.6
MsG0280007119.01.T01 MTR_6g021880 31.977 172 101 8 1 172 227 382 6.53e-13 66.6
MsG0280007119.01.T01 MTR_7g407070 31.792 173 97 6 2 172 246 399 1.50e-12 65.5
MsG0280007119.01.T01 MTR_3g009300 30.508 177 96 8 2 174 202 355 2.37e-12 65.1
MsG0280007119.01.T01 MTR_2g046830 31.250 160 92 6 2 155 220 367 3.24e-12 64.7
MsG0280007119.01.T01 MTR_3g011100 32.065 184 90 9 2 172 441 602 6.51e-12 63.9
MsG0280007119.01.T01 MTR_3g007330 27.869 183 93 6 1 174 243 395 1.59e-11 62.8
MsG0280007119.01.T01 MTR_3g011020 40.404 99 46 4 2 100 220 305 1.95e-11 62.4
MsG0280007119.01.T01 MTR_3g009310 31.073 177 88 9 2 172 243 391 2.43e-11 62.0
MsG0280007119.01.T01 MTR_2g029990 30.286 175 98 9 2 172 249 403 3.51e-11 61.6
MsG0280007119.01.T01 MTR_3g006640 33.333 147 81 6 2 141 131 267 3.59e-11 61.2
MsG0280007119.01.T01 MTR_2g046920 30.556 180 94 8 2 174 220 375 4.29e-11 61.2
MsG0280007119.01.T01 MTR_3g030240 31.250 176 90 8 2 172 229 378 5.37e-11 61.2
MsG0280007119.01.T01 MTR_3g013770 30.508 177 92 8 2 173 239 389 5.44e-11 61.2
MsG0280007119.01.T01 MTR_5g056990 36.893 103 44 4 2 100 90 175 9.17e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007119.01.T01 AT4G12560 37.173 191 107 3 2 191 212 390 1.91e-31 118
MsG0280007119.01.T01 AT4G12560 37.173 191 107 3 2 191 212 390 1.91e-31 118
MsG0280007119.01.T01 AT4G12560 37.173 191 107 3 2 191 212 390 1.91e-31 118
MsG0280007119.01.T01 AT4G22390 33.333 144 85 2 2 144 211 344 9.39e-18 80.5
MsG0280007119.01.T01 AT4G22390 33.333 144 85 2 2 144 211 344 9.52e-18 80.5

Find 35 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATCCTTCATTCAAATT+AGG 0.210119 2:-10886824 None:intergenic
AGAACTTTCTGTTGCTGTTT+TGG 0.229110 2:+10886517 MsG0280007119.01.T01:CDS
TTGATTTCTGTGAGGCCTTT+AGG 0.240446 2:+10886701 MsG0280007119.01.T01:CDS
GTTCCTCTTCCTGATGAAAT+TGG 0.265276 2:+10886488 MsG0280007119.01.T01:CDS
AAACTACTAAAATAGATGTT+TGG 0.316493 2:+10886603 MsG0280007119.01.T01:CDS
TTATTCTAGTGATGGAAATA+AGG 0.337869 2:+10886724 MsG0280007119.01.T01:CDS
CCTTCCATGTTGATAATTGT+AGG 0.350854 2:+10886894 MsG0280007119.01.T01:CDS
ATCATAGCTCCATTCATATT+AGG 0.353494 2:-10886845 None:intergenic
AACTACTAAAATAGATGTTT+GGG 0.360391 2:+10886604 MsG0280007119.01.T01:CDS
TGATGGAAATAAGGTTCTAT+TGG 0.379061 2:+10886733 MsG0280007119.01.T01:CDS
CAATGAAAATCTCAAGTGTT+AGG 0.392998 2:-10886463 None:intergenic
GAACAAGTTAGTTGTGTTGA+AGG 0.395270 2:+10886797 MsG0280007119.01.T01:CDS
GTTTGGGTGATGAAAGAATA+TGG 0.400967 2:+10886620 MsG0280007119.01.T01:CDS
GGAATTCCTAATTTGAATGA+AGG 0.405039 2:+10886818 MsG0280007119.01.T01:CDS
GGAAATAGATTGTATGAAGC+TGG 0.412006 2:+10886754 MsG0280007119.01.T01:CDS
TAGATTGTATGAAGCTGGTT+TGG 0.428185 2:+10886759 MsG0280007119.01.T01:CDS
TTGTTGAGAATTCTCTTCAC+TGG 0.446412 2:+10886387 MsG0280007119.01.T01:CDS
TCTTCACCTCCAATTTCATC+AGG 0.451712 2:-10886497 None:intergenic
GAAGGATTTCCTAATATGAA+TGG 0.487943 2:+10886836 MsG0280007119.01.T01:CDS
CCTTTAGGTTATTCTAGTGA+TGG 0.497547 2:+10886716 MsG0280007119.01.T01:CDS
CTCTTCACTGGATAATGTCT+CGG 0.505952 2:+10886399 MsG0280007119.01.T01:CDS
CCTCTTCCTGATGAAATTGG+AGG 0.507562 2:+10886491 MsG0280007119.01.T01:CDS
CCTCCAATTTCATCAGGAAG+AGG 0.533158 2:-10886491 None:intergenic
TGTTGATAATTGTAGGAAGA+AGG 0.538310 2:+10886901 MsG0280007119.01.T01:CDS
CCATCACTAGAATAACCTAA+AGG 0.581490 2:-10886716 None:intergenic
AATATGGATCTAGAGATAGT+TGG 0.584921 2:+10886636 MsG0280007119.01.T01:CDS
CATGGAAGGAAGGTGGCACA+AGG 0.595998 2:-10886880 None:intergenic
CCTACAATTATCAACATGGA+AGG 0.602007 2:-10886894 None:intergenic
TCTTCCTACAATTATCAACA+TGG 0.612083 2:-10886898 None:intergenic
CAATTATCAACATGGAAGGA+AGG 0.619789 2:-10886890 None:intergenic
ATACAAGCAAGAGCAAGAGA+AGG 0.632854 2:+10886930 MsG0280007119.01.T01:CDS
ATTTATCTTTGATTTCTGTG+AGG 0.634434 2:+10886693 MsG0280007119.01.T01:CDS
TTAAACGCGACAATTAAACA+AGG 0.677389 2:-10886440 None:intergenic
TTATCAACATGGAAGGAAGG+TGG 0.689140 2:-10886887 None:intergenic
TAAACGCGACAATTAAACAA+GGG 0.729501 2:-10886439 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTACTAAAATAGATGTT+TGG + Chr2:10886603-10886622 MsG0280007119.01.T01:CDS 20.0%
!! AACTACTAAAATAGATGTTT+GGG + Chr2:10886604-10886623 MsG0280007119.01.T01:CDS 20.0%
!! ATTTATCTTTGATTTCTGTG+AGG + Chr2:10886693-10886712 MsG0280007119.01.T01:CDS 25.0%
!! TTATTCTAGTGATGGAAATA+AGG + Chr2:10886724-10886743 MsG0280007119.01.T01:CDS 25.0%
AATATGGATCTAGAGATAGT+TGG + Chr2:10886636-10886655 MsG0280007119.01.T01:CDS 30.0%
ATCATAGCTCCATTCATATT+AGG - Chr2:10886848-10886867 None:intergenic 30.0%
CAATGAAAATCTCAAGTGTT+AGG - Chr2:10886466-10886485 None:intergenic 30.0%
GAAGGATTTCCTAATATGAA+TGG + Chr2:10886836-10886855 MsG0280007119.01.T01:CDS 30.0%
GGAAATCCTTCATTCAAATT+AGG - Chr2:10886827-10886846 None:intergenic 30.0%
GGAATTCCTAATTTGAATGA+AGG + Chr2:10886818-10886837 MsG0280007119.01.T01:CDS 30.0%
TAAACGCGACAATTAAACAA+GGG - Chr2:10886442-10886461 None:intergenic 30.0%
TCTTCCTACAATTATCAACA+TGG - Chr2:10886901-10886920 None:intergenic 30.0%
TGATGGAAATAAGGTTCTAT+TGG + Chr2:10886733-10886752 MsG0280007119.01.T01:CDS 30.0%
TTAAACGCGACAATTAAACA+AGG - Chr2:10886443-10886462 None:intergenic 30.0%
! AACACTTGAGATTTTCATTG+AGG + Chr2:10886466-10886485 MsG0280007119.01.T01:CDS 30.0%
! TTGGTGTAAACTTTTTACGA+TGG + Chr2:10886655-10886674 MsG0280007119.01.T01:CDS 30.0%
!! TGTTGATAATTGTAGGAAGA+AGG + Chr2:10886901-10886920 MsG0280007119.01.T01:CDS 30.0%
!!! ACACTTTTTGTTGCTGTTTT+GGG + Chr2:10886551-10886570 MsG0280007119.01.T01:CDS 30.0%
!!! TACACTTTTTGTTGCTGTTT+TGG + Chr2:10886550-10886569 MsG0280007119.01.T01:CDS 30.0%
CAATTATCAACATGGAAGGA+AGG - Chr2:10886893-10886912 None:intergenic 35.0%
CCATCACTAGAATAACCTAA+AGG - Chr2:10886719-10886738 None:intergenic 35.0%
CCTACAATTATCAACATGGA+AGG - Chr2:10886897-10886916 None:intergenic 35.0%
GAACAAGTTAGTTGTGTTGA+AGG + Chr2:10886797-10886816 MsG0280007119.01.T01:CDS 35.0%
GGAAATAGATTGTATGAAGC+TGG + Chr2:10886754-10886773 MsG0280007119.01.T01:CDS 35.0%
GTTTGGGTGATGAAAGAATA+TGG + Chr2:10886620-10886639 MsG0280007119.01.T01:CDS 35.0%
TAGATTGTATGAAGCTGGTT+TGG + Chr2:10886759-10886778 MsG0280007119.01.T01:CDS 35.0%
TTGTTGAGAATTCTCTTCAC+TGG + Chr2:10886387-10886406 MsG0280007119.01.T01:CDS 35.0%
! CCTTTAGGTTATTCTAGTGA+TGG + Chr2:10886716-10886735 MsG0280007119.01.T01:CDS 35.0%
!! CCTTCCATGTTGATAATTGT+AGG + Chr2:10886894-10886913 MsG0280007119.01.T01:CDS 35.0%
!!! AGAACTTTCTGTTGCTGTTT+TGG + Chr2:10886517-10886536 MsG0280007119.01.T01:CDS 35.0%
!!! GAACTTTCTGTTGCTGTTTT+GGG + Chr2:10886518-10886537 MsG0280007119.01.T01:CDS 35.0%
!!! TTTTTGTTGCTGTTTTGGGA+GGG + Chr2:10886555-10886574 MsG0280007119.01.T01:CDS 35.0%
ATACAAGCAAGAGCAAGAGA+AGG + Chr2:10886930-10886949 MsG0280007119.01.T01:CDS 40.0%
CTCTTCACTGGATAATGTCT+CGG + Chr2:10886399-10886418 MsG0280007119.01.T01:CDS 40.0%
GTTCCTCTTCCTGATGAAAT+TGG + Chr2:10886488-10886507 MsG0280007119.01.T01:CDS 40.0%
TCTTCACCTCCAATTTCATC+AGG - Chr2:10886500-10886519 None:intergenic 40.0%
TTATCAACATGGAAGGAAGG+TGG - Chr2:10886890-10886909 None:intergenic 40.0%
TTGATTTCTGTGAGGCCTTT+AGG + Chr2:10886701-10886720 MsG0280007119.01.T01:CDS 40.0%
!!! CTTTTTGTTGCTGTTTTGGG+AGG + Chr2:10886554-10886573 MsG0280007119.01.T01:CDS 40.0%
CCTCCAATTTCATCAGGAAG+AGG - Chr2:10886494-10886513 None:intergenic 45.0%
CCTCTTCCTGATGAAATTGG+AGG + Chr2:10886491-10886510 MsG0280007119.01.T01:CDS 45.0%
CATGGAAGGAAGGTGGCACA+AGG - Chr2:10886883-10886902 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 10886377 10886973 10886377 ID=MsG0280007119.01;Name=MsG0280007119.01
Chr2 mRNA 10886377 10886973 10886377 ID=MsG0280007119.01.T01;Parent=MsG0280007119.01;Name=MsG0280007119.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|198
Chr2 exon 10886377 10886973 10886377 ID=MsG0280007119.01.T01:exon:2577;Parent=MsG0280007119.01.T01
Chr2 CDS 10886377 10886973 10886377 ID=MsG0280007119.01.T01:cds;Parent=MsG0280007119.01.T01
Gene Sequence

>MsG0280007119.01.T01

ATGGGGGTTTTTGTTGAGAATTCTCTTCACTGGATAATGTCTCGGAAACTTGATGTGTCACACCCTTGTTTAATTGTCGCGTTTAACCTAACACTTGAGATTTTCATTGAGGTTCCTCTTCCTGATGAAATTGGAGGTGAAGAACTTTCTGTTGCTGTTTTGGGAAAGTCTATTACACTTTTTGTTGCTGTTTTGGGAGGGTGTCTTTGTATGATTGTGAATTATCAAACTACTAAAATAGATGTTTGGGTGATGAAAGAATATGGATCTAGAGATAGTTGGTGTAAACTTTTTACGATGGTTAAATCGTGTTTCGATTTATCTTTGATTTCTGTGAGGCCTTTAGGTTATTCTAGTGATGGAAATAAGGTTCTATTGGAAATAGATTGTATGAAGCTGGTTTGGTATGATTTGAAGAGTGAACAAGTTAGTTGTGTTGAAGGAATTCCTAATTTGAATGAAGGATTTCCTAATATGAATGGAGCTATGATTTGTGTTGAAAGCCTTGTGCCACCTTCCTTCCATGTTGATAATTGTAGGAAGAAGGAGAATCATACAAGCAAGAGCAAGAGAAGGTACTTCTTATTGATTATCTGA

Protein sequence

>MsG0280007119.01.T01

MGVFVENSLHWIMSRKLDVSHPCLIVAFNLTLEIFIEVPLPDEIGGEELSVAVLGKSITLFVAVLGGCLCMIVNYQTTKIDVWVMKEYGSRDSWCKLFTMVKSCFDLSLISVRPLGYSSDGNKVLLEIDCMKLVWYDLKSEQVSCVEGIPNLNEGFPNMNGAMICVESLVPPSFHVDNCRKKENHTSKSKRRYFLLII*