Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007154.01.T01 | XP_024631570.1 | 36.325 | 234 | 84 | 3 | 1 | 234 | 29 | 197 | 9.88E-26 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007154.01.T01 | A0A396J6Z8 | 36.325 | 234 | 84 | 3 | 1 | 234 | 29 | 197 | 4.72e-26 | 110 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007154.01 | MsG0880042519.01 | 0.814111 | 1.819158e-51 | 1.209856e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007154.01.T01 | MTR_2g021480 | 64.789 | 71 | 25 | 0 | 1 | 71 | 29 | 99 | 1.80e-23 | 95.9 |
| MsG0280007154.01.T01 | MTR_2g021560 | 65.753 | 73 | 25 | 0 | 1 | 73 | 18 | 90 | 4.81e-23 | 94.7 |
| MsG0280007154.01.T01 | MTR_2g020500 | 40.000 | 160 | 61 | 2 | 1 | 160 | 29 | 153 | 1.94e-21 | 89.4 |
| MsG0280007154.01.T01 | MTR_2g021630 | 34.188 | 234 | 68 | 3 | 1 | 234 | 18 | 165 | 3.94e-20 | 84.3 |
| MsG0280007154.01.T01 | MTR_1g025830 | 37.131 | 237 | 111 | 5 | 1 | 226 | 14 | 223 | 5.13e-19 | 82.8 |
| MsG0280007154.01.T01 | MTR_2g021600 | 34.649 | 228 | 82 | 4 | 1 | 228 | 20 | 180 | 1.11e-18 | 82.4 |
| MsG0280007154.01.T01 | MTR_2g021530 | 60.656 | 61 | 24 | 0 | 1 | 61 | 14 | 74 | 3.92e-18 | 77.8 |
| MsG0280007154.01.T01 | MTR_5g048270 | 33.613 | 238 | 139 | 5 | 1 | 235 | 14 | 235 | 4.51e-18 | 80.9 |
| MsG0280007154.01.T01 | MTR_2g021510 | 61.905 | 63 | 23 | 1 | 1 | 63 | 28 | 89 | 6.36e-17 | 76.3 |
| MsG0280007154.01.T01 | MTR_3g087310 | 59.322 | 59 | 24 | 0 | 1 | 59 | 18 | 76 | 1.67e-16 | 72.4 |
| MsG0280007154.01.T01 | MTR_0485s0020 | 54.688 | 64 | 29 | 0 | 1 | 64 | 18 | 81 | 2.80e-16 | 74.7 |
| MsG0280007154.01.T01 | MTR_1g048260 | 36.872 | 179 | 106 | 3 | 1 | 175 | 14 | 189 | 9.87e-16 | 73.9 |
| MsG0280007154.01.T01 | MTR_1g025810 | 36.471 | 255 | 109 | 8 | 1 | 226 | 14 | 244 | 2.68e-15 | 73.2 |
| MsG0280007154.01.T01 | MTR_2g021570 | 66.667 | 51 | 17 | 0 | 1 | 51 | 40 | 90 | 4.18e-14 | 66.6 |
| MsG0280007154.01.T01 | MTR_2g021660 | 58.929 | 56 | 23 | 0 | 1 | 56 | 19 | 74 | 3.86e-13 | 65.9 |
| MsG0280007154.01.T01 | MTR_2g021450 | 51.786 | 56 | 27 | 0 | 1 | 56 | 18 | 73 | 1.71e-12 | 63.5 |
| MsG0280007154.01.T01 | MTR_2g021590 | 50.562 | 89 | 38 | 1 | 1 | 89 | 22 | 104 | 3.91e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 53 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCACTTGGATGATATTT+TGG | 0.211914 | 2:-11228274 | MsG0280007154.01.T01:CDS |
| CAAAACTCTTAGAGTTAATA+TGG | 0.252126 | 2:-11228127 | MsG0280007154.01.T01:CDS |
| AGAGGAAGAGGAGGAGGAGG+AGG | 0.325244 | 2:-11228391 | MsG0280007154.01.T01:CDS |
| CGAGATTATTCATAAAAGTC+TGG | 0.356984 | 2:+11228550 | None:intergenic |
| GGAAGAGGAAGAGGAGGAGG+AGG | 0.358190 | 2:-11228394 | MsG0280007154.01.T01:CDS |
| AGAGGAAGAGGAAGAGGAGG+AGG | 0.362413 | 2:-11228397 | MsG0280007154.01.T01:CDS |
| AGAAGAAGAGGAAGAGGAAG+AGG | 0.377053 | 2:-11228445 | MsG0280007154.01.T01:CDS |
| AGAGGAAGAGGAAGAGGAAG+AGG | 0.377949 | 2:-11228403 | MsG0280007154.01.T01:CDS |
| CGAGGAGGTGGAGGAGGAGG+AGG | 0.394406 | 2:-11228355 | MsG0280007154.01.T01:CDS |
| AGAGGAAGAGGAAGAGGAAG+AGG | 0.394447 | 2:-11228409 | MsG0280007154.01.T01:CDS |
| AGAGGAAGAGGAAGAGGAAG+AGG | 0.394447 | 2:-11228415 | MsG0280007154.01.T01:CDS |
| AGAGGAAGAGGAAGAGGAAG+AGG | 0.394447 | 2:-11228421 | MsG0280007154.01.T01:CDS |
| AGAGGAAGAGGAAGAGGAAG+AGG | 0.394447 | 2:-11228427 | MsG0280007154.01.T01:CDS |
| AGAGGAAGAGGAAGAGGAAG+AGG | 0.394447 | 2:-11228433 | MsG0280007154.01.T01:CDS |
| AGAGGAAGAGGAAGAGGAAG+AGG | 0.394447 | 2:-11228439 | MsG0280007154.01.T01:CDS |
| ATAATCGAAGGGTGGCTGTT+AGG | 0.428129 | 2:-11228173 | MsG0280007154.01.T01:CDS |
| CGACGAGGAGGTGGAGGAGG+AGG | 0.428580 | 2:-11228358 | MsG0280007154.01.T01:CDS |
| GGAAGAGGAAGAGGAAGAGG+AGG | 0.467961 | 2:-11228400 | MsG0280007154.01.T01:CDS |
| TGCTCAATTCAACCCTGGAC+AGG | 0.470202 | 2:+11228718 | None:intergenic |
| TCGCCATCTTCTTGTTCTTG+TGG | 0.492727 | 2:+11228522 | None:intergenic |
| GGACGACGACGACGAGGAGG+TGG | 0.499501 | 2:-11228367 | MsG0280007154.01.T01:CDS |
| CACAAGCTCTGCTACTTGAG+GGG | 0.505118 | 2:-11228197 | MsG0280007154.01.T01:CDS |
| TGATGATCGAAATCCTGTCC+AGG | 0.505344 | 2:-11228731 | MsG0280007154.01.T01:CDS |
| CTGTTCTATTAGTGAAGGCC+AGG | 0.505429 | 2:-11228596 | MsG0280007154.01.T01:CDS |
| GGCGATGCTGAAGGAGGAGG+AGG | 0.507834 | 2:-11228504 | MsG0280007154.01.T01:CDS |
| GATGGCGATGCTGAAGGAGG+AGG | 0.511050 | 2:-11228507 | MsG0280007154.01.T01:CDS |
| CGACGACGAGGAGGTGGAGG+AGG | 0.512647 | 2:-11228361 | MsG0280007154.01.T01:CDS |
| GAAAGCATTGTAGTGTTGCC+TGG | 0.522580 | 2:+11228578 | None:intergenic |
| TGAGGCGCGTTTGCAAGTTG+TGG | 0.523592 | 2:-11228683 | MsG0280007154.01.T01:CDS |
| CGACGACGACGAGGAGGTGG+AGG | 0.527934 | 2:-11228364 | MsG0280007154.01.T01:CDS |
| ACACAAGCTCTGCTACTTGA+GGG | 0.529243 | 2:-11228198 | MsG0280007154.01.T01:CDS |
| TGAGCAATCCTCTGCAATTG+AGG | 0.540428 | 2:-11228701 | MsG0280007154.01.T01:CDS |
| CAAGAAGATGGCGATGCTGA+AGG | 0.548385 | 2:-11228513 | MsG0280007154.01.T01:CDS |
| GAGATTATTCATAAAAGTCT+GGG | 0.551075 | 2:+11228551 | None:intergenic |
| AGTTAATATGGAAGCTGTGG+AGG | 0.559378 | 2:-11228115 | MsG0280007154.01.T01:CDS |
| GATGAATGTAGTTGCTCACT+TGG | 0.563629 | 2:-11228286 | MsG0280007154.01.T01:CDS |
| AACACAAGCTCTGCTACTTG+AGG | 0.570715 | 2:-11228199 | MsG0280007154.01.T01:CDS |
| TAGAGTTAATATGGAAGCTG+TGG | 0.579637 | 2:-11228118 | MsG0280007154.01.T01:CDS |
| GCTGTTGAAGAAGAAGAAGC+TGG | 0.589808 | 2:-11228327 | MsG0280007154.01.T01:CDS |
| ATGCCACAAGAACAAGAAGA+TGG | 0.596814 | 2:-11228525 | MsG0280007154.01.T01:CDS |
| AATCACTGTTCTATTAGTGA+AGG | 0.604717 | 2:-11228601 | MsG0280007154.01.T01:CDS |
| TCGGTTACTCAAAGAGGACA+TGG | 0.615506 | 2:-11228244 | MsG0280007154.01.T01:CDS |
| GGAGGAGGACGACGACGACG+AGG | 0.617117 | 2:-11228373 | MsG0280007154.01.T01:CDS |
| CAAACGCGCCTCAATTGCAG+AGG | 0.619830 | 2:+11228693 | None:intergenic |
| GGATGTTCGGTTACTCAAAG+AGG | 0.620705 | 2:-11228250 | MsG0280007154.01.T01:CDS |
| GAAGATGGCGATGCTGAAGG+AGG | 0.622497 | 2:-11228510 | MsG0280007154.01.T01:CDS |
| GTTAGGCGTATCGATATCGA+AGG | 0.628879 | 2:-11228156 | MsG0280007154.01.T01:CDS |
| ACAAGCTCTGCTACTTGAGG+GGG | 0.633999 | 2:-11228196 | MsG0280007154.01.T01:CDS |
| AGGATTGCTCAATTCAACCC+TGG | 0.634319 | 2:+11228713 | None:intergenic |
| GATGATCGAAATCCTGTCCA+GGG | 0.640383 | 2:-11228730 | MsG0280007154.01.T01:CDS |
| GGAGGACGACGACGACGAGG+AGG | 0.662884 | 2:-11228370 | MsG0280007154.01.T01:CDS |
| TTTCAGTGTGAGATCTGTGA+AGG | 0.682183 | 2:+11228622 | None:intergenic |
| TTAGGCGTATCGATATCGAA+GGG | 0.723170 | 2:-11228155 | MsG0280007154.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAAAACTCTTAGAGTTAATA+TGG | - | Chr2:11228640-11228659 | MsG0280007154.01.T01:CDS | 25.0% |
| ! | GAGATTATTCATAAAAGTCT+GGG | + | Chr2:11228219-11228238 | None:intergenic | 25.0% |
| AATCACTGTTCTATTAGTGA+AGG | - | Chr2:11228166-11228185 | MsG0280007154.01.T01:CDS | 30.0% | |
| CGAGATTATTCATAAAAGTC+TGG | + | Chr2:11228220-11228239 | None:intergenic | 30.0% | |
| !!! | ATGATATTTTGGTGGATGTT+CGG | - | Chr2:11228504-11228523 | MsG0280007154.01.T01:CDS | 30.0% |
| AGAAGAAGAAGAAGAAGAAG+AGG | - | Chr2:11228286-11228305 | MsG0280007154.01.T01:CDS | 35.0% | |
| TAGAGTTAATATGGAAGCTG+TGG | - | Chr2:11228649-11228668 | MsG0280007154.01.T01:CDS | 35.0% | |
| ! | TGCTCACTTGGATGATATTT+TGG | - | Chr2:11228493-11228512 | MsG0280007154.01.T01:CDS | 35.0% |
| !! | TCACTTGGATGATATTTTGG+TGG | - | Chr2:11228496-11228515 | MsG0280007154.01.T01:CDS | 35.0% |
| AGAAGAAGAAGAAGAGGAAG+AGG | - | Chr2:11228316-11228335 | MsG0280007154.01.T01:CDS | 40.0% | |
| AGTTAATATGGAAGCTGTGG+AGG | - | Chr2:11228652-11228671 | MsG0280007154.01.T01:CDS | 40.0% | |
| ATGCCACAAGAACAAGAAGA+TGG | - | Chr2:11228242-11228261 | MsG0280007154.01.T01:CDS | 40.0% | |
| GATGAATGTAGTTGCTCACT+TGG | - | Chr2:11228481-11228500 | MsG0280007154.01.T01:CDS | 40.0% | |
| TTTCAGTGTGAGATCTGTGA+AGG | + | Chr2:11228148-11228167 | None:intergenic | 40.0% | |
| ! | TTAGGCGTATCGATATCGAA+GGG | - | Chr2:11228612-11228631 | MsG0280007154.01.T01:CDS | 40.0% |
| ACTTGAGGGGGATAATCGAA+GGG | - | Chr2:11228583-11228602 | MsG0280007154.01.T01:CDS | 45.0% | |
| AGAAGAAGAGGAAGAGGAAG+AGG | - | Chr2:11228322-11228341 | MsG0280007154.01.T01:CDS | 45.0% | |
| AGGATTGCTCAATTCAACCC+TGG | + | Chr2:11228057-11228076 | None:intergenic | 45.0% | |
| ATAATCGAAGGGTGGCTGTT+AGG | - | Chr2:11228594-11228613 | MsG0280007154.01.T01:CDS | 45.0% | |
| CTGTTCTATTAGTGAAGGCC+AGG | - | Chr2:11228171-11228190 | MsG0280007154.01.T01:CDS | 45.0% | |
| GCTGTTGAAGAAGAAGAAGC+TGG | - | Chr2:11228440-11228459 | MsG0280007154.01.T01:CDS | 45.0% | |
| GGATGTTCGGTTACTCAAAG+AGG | - | Chr2:11228517-11228536 | MsG0280007154.01.T01:CDS | 45.0% | |
| TACTTGAGGGGGATAATCGA+AGG | - | Chr2:11228582-11228601 | MsG0280007154.01.T01:CDS | 45.0% | |
| TCGCCATCTTCTTGTTCTTG+TGG | + | Chr2:11228248-11228267 | None:intergenic | 45.0% | |
| TCGGTTACTCAAAGAGGACA+TGG | - | Chr2:11228523-11228542 | MsG0280007154.01.T01:CDS | 45.0% | |
| TGAGCAATCCTCTGCAATTG+AGG | - | Chr2:11228066-11228085 | MsG0280007154.01.T01:CDS | 45.0% | |
| ! | AACACAAGCTCTGCTACTTG+AGG | - | Chr2:11228568-11228587 | MsG0280007154.01.T01:CDS | 45.0% |
| ! | ACACAAGCTCTGCTACTTGA+GGG | - | Chr2:11228569-11228588 | MsG0280007154.01.T01:CDS | 45.0% |
| ! | GTTAGGCGTATCGATATCGA+AGG | - | Chr2:11228611-11228630 | MsG0280007154.01.T01:CDS | 45.0% |
| !! | GAAAGCATTGTAGTGTTGCC+TGG | + | Chr2:11228192-11228211 | None:intergenic | 45.0% |
| AGAGGAAGAGGAAGAGGAAG+AGG | - | Chr2:11228328-11228347 | MsG0280007154.01.T01:CDS | 50.0% | |
| CAAGAAGATGGCGATGCTGA+AGG | - | Chr2:11228254-11228273 | MsG0280007154.01.T01:CDS | 50.0% | |
| TGCTCAATTCAACCCTGGAC+AGG | + | Chr2:11228052-11228071 | None:intergenic | 50.0% | |
| ! | ACAAGCTCTGCTACTTGAGG+GGG | - | Chr2:11228571-11228590 | MsG0280007154.01.T01:CDS | 50.0% |
| ! | CACAAGCTCTGCTACTTGAG+GGG | - | Chr2:11228570-11228589 | MsG0280007154.01.T01:CDS | 50.0% |
| AGAGGAAGAGGAAGAGGAGG+AGG | - | Chr2:11228370-11228389 | MsG0280007154.01.T01:CDS | 55.0% | |
| CAAACGCGCCTCAATTGCAG+AGG | + | Chr2:11228077-11228096 | None:intergenic | 55.0% | |
| GAAGATGGCGATGCTGAAGG+AGG | - | Chr2:11228257-11228276 | MsG0280007154.01.T01:CDS | 55.0% | |
| GGAAGAGGAAGAGGAAGAGG+AGG | - | Chr2:11228331-11228350 | MsG0280007154.01.T01:CDS | 55.0% | |
| TGAGGCGCGTTTGCAAGTTG+TGG | - | Chr2:11228084-11228103 | MsG0280007154.01.T01:CDS | 55.0% | |
| TGAGGGGGATAATCGAAGGG+TGG | - | Chr2:11228586-11228605 | MsG0280007154.01.T01:CDS | 55.0% | |
| AGAGGAAGAGGAGGAGGAGG+AGG | - | Chr2:11228376-11228395 | MsG0280007154.01.T01:CDS | 60.0% | |
| GATGGCGATGCTGAAGGAGG+AGG | - | Chr2:11228260-11228279 | MsG0280007154.01.T01:CDS | 60.0% | |
| GGAAGAGGAAGAGGAGGAGG+AGG | - | Chr2:11228373-11228392 | MsG0280007154.01.T01:CDS | 60.0% | |
| GGAAGAGGAGGAGGAGGAGG+AGG | - | Chr2:11228379-11228398 | MsG0280007154.01.T01:CDS | 65.0% | |
| GGCGATGCTGAAGGAGGAGG+AGG | - | Chr2:11228263-11228282 | MsG0280007154.01.T01:CDS | 65.0% | |
| CGACGACGACGAGGAGGTGG+AGG | - | Chr2:11228403-11228422 | MsG0280007154.01.T01:CDS | 70.0% | |
| CGACGACGAGGAGGTGGAGG+AGG | - | Chr2:11228406-11228425 | MsG0280007154.01.T01:CDS | 70.0% | |
| CGACGAGGAGGTGGAGGAGG+AGG | - | Chr2:11228409-11228428 | MsG0280007154.01.T01:CDS | 70.0% | |
| CGAGGAGGTGGAGGAGGAGG+AGG | - | Chr2:11228412-11228431 | MsG0280007154.01.T01:CDS | 70.0% | |
| GGACGACGACGACGAGGAGG+TGG | - | Chr2:11228400-11228419 | MsG0280007154.01.T01:CDS | 70.0% | |
| GGAGGACGACGACGACGAGG+AGG | - | Chr2:11228397-11228416 | MsG0280007154.01.T01:CDS | 70.0% | |
| GGAGGAGGACGACGACGACG+AGG | - | Chr2:11228394-11228413 | MsG0280007154.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 11228038 | 11228751 | 11228038 | ID=MsG0280007154.01;Name=MsG0280007154.01 |
| Chr2 | mRNA | 11228038 | 11228751 | 11228038 | ID=MsG0280007154.01.T01;Parent=MsG0280007154.01;Name=MsG0280007154.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|237 |
| Chr2 | exon | 11228038 | 11228751 | 11228038 | ID=MsG0280007154.01.T01:exon:311;Parent=MsG0280007154.01.T01 |
| Chr2 | CDS | 11228038 | 11228751 | 11228038 | ID=MsG0280007154.01.T01:cds;Parent=MsG0280007154.01.T01 |
| Gene Sequence |
| Protein sequence |
