AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007210.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007210.01.T01 MTR_2g022660 94.975 199 0 1 1 189 1 199 5.21e-136 378
MsG0280007210.01.T01 MTR_6g087990 43.966 116 64 1 71 186 45 159 1.17e-27 102
MsG0280007210.01.T01 MTR_7g103610 40.678 118 67 2 71 186 98 214 2.14e-23 92.8
MsG0280007210.01.T01 MTR_7g103610 40.952 105 60 1 71 173 98 202 2.82e-22 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007210.01.T01 AT3G06050 74.138 174 35 1 26 189 28 201 1.20e-91 266
MsG0280007210.01.T01 AT3G06050 67.890 109 25 1 26 124 28 136 1.57e-48 156
MsG0280007210.01.T01 AT1G65980 40.517 116 68 1 71 186 45 159 1.72e-22 89.0
MsG0280007210.01.T01 AT1G60740 39.655 116 69 1 71 186 45 159 4.46e-22 88.2
MsG0280007210.01.T01 AT1G65970 38.793 116 70 1 71 186 45 159 5.46e-21 85.1
MsG0280007210.01.T01 AT3G52960 38.462 104 62 1 71 172 115 218 1.83e-19 82.8

Find 52 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTATCAATGACATCTTTA+AGG 0.095553 2:+11967858 MsG0280007210.01.T01:CDS
CATCCCAATTACGAGCTTTC+TGG 0.124781 2:-11967807 None:intergenic
CCATCTGATGTCAAGGTTTC+TGG 0.141794 2:+11971112 MsG0280007210.01.T01:CDS
TTCTGGTGCAGAAACCATTT+TGG 0.209676 2:+11971129 MsG0280007210.01.T01:CDS
CTCTCCAGAAAGCTCGTAAT+TGG 0.220729 2:+11967803 MsG0280007210.01.T01:CDS
GATAGGAGTGGTAGAGAATT+TGG 0.240074 2:-11967842 None:intergenic
GGATAATATTGAGAAGTTTA+AGG 0.249698 2:+11969027 MsG0280007210.01.T01:CDS
ACTTCAAATTTGTCCCAAAA+TGG 0.266725 2:-11971143 None:intergenic
GCCCATGCATTCACAGTATA+TGG 0.292751 2:-11969091 None:intergenic
AGATGTATGAAAGGAATTTA+TGG 0.308647 2:-11967589 None:intergenic
CTTCAGTTCTTCATGGAATC+AGG 0.312050 2:+11967725 MsG0280007210.01.T01:CDS
GAAAACGCTTACAGCATCTT+TGG 0.314545 2:-11969128 None:intergenic
TCAGTGTCTTCAGTTCTTCA+TGG 0.351126 2:+11967718 MsG0280007210.01.T01:CDS
AAAATGTACCTGATGCTTCC+AGG 0.374565 2:+11971233 MsG0280007210.01.T01:three_prime_UTR
ATTGAGAAGTTTAAGGCTAA+AGG 0.390373 2:+11969034 MsG0280007210.01.T01:CDS
TCTTACGCAAAGGTTGCAAC+TGG 0.410870 2:+11967748 MsG0280007210.01.T01:CDS
TTCTCAATATTATCCTTGTA+AGG 0.411089 2:-11969019 None:intergenic
AAATATTCTATCAGCTCAGC+TGG 0.430858 2:-11971259 None:intergenic
TGGTCAGCATATGTGGTAGA+TGG 0.435531 2:+11971058 MsG0280007210.01.T01:CDS
GATCTCTCTGGTGCTCTGCT+TGG 0.444411 2:+11970755 MsG0280007210.01.T01:CDS
TATCAGCTCAGCTGGAAGCC+TGG 0.450762 2:-11971251 None:intergenic
GCTGGAAGCCTGGAAGCATC+AGG 0.461347 2:-11971241 None:intergenic
TCTCCAGAAAGCTCGTAATT+GGG 0.463629 2:+11967804 MsG0280007210.01.T01:CDS
TGTGGTAGATGGAAAGGTTA+AGG 0.463992 2:+11971069 MsG0280007210.01.T01:CDS
TGGGAGCTTTCACAAAAGCT+TGG 0.489869 2:+11970721 MsG0280007210.01.T01:CDS
CAAACATGTTCCTCCTTACA+AGG 0.489949 2:+11969006 MsG0280007210.01.T01:CDS
GAATTAACTACTGATCTCTC+TGG 0.503738 2:+11970743 MsG0280007210.01.T01:CDS
CTGGTGCTCTGCTTGGAACC+CGG 0.509965 2:+11970762 MsG0280007210.01.T01:CDS
CCAGAAACCTTGACATCAGA+TGG 0.516805 2:-11971112 None:intergenic
ACCTTAAAGATGTCATTGAT+AGG 0.525640 2:-11967859 None:intergenic
TGATTCTGTTATTTGCGTGT+CGG 0.528094 2:+11969060 MsG0280007210.01.T01:CDS
ATCTCTTAGGGTGCATACAC+AGG 0.531205 2:+11968971 MsG0280007210.01.T01:intron
AAAGCTCGTAATTGGGATGA+AGG 0.533139 2:+11967811 MsG0280007210.01.T01:CDS
ATCCATATACTGTGAATGCA+TGG 0.534538 2:+11969089 MsG0280007210.01.T01:CDS
TGCTTGGAACCCGGTCTGAA+AGG 0.539759 2:+11970771 MsG0280007210.01.T01:CDS
AGCTTTCTGGAGAGAAACAT+TGG 0.544296 2:-11967794 None:intergenic
GTGTAGATGGTCAGCATATG+TGG 0.555542 2:+11971051 MsG0280007210.01.T01:intron
AGCATATGTGGTAGATGGAA+AGG 0.556118 2:+11971063 MsG0280007210.01.T01:CDS
ATATAATATACCTTTCAGAC+CGG 0.564202 2:-11970781 None:intergenic
AGAGACCACAGATGTATGAA+AGG 0.565198 2:-11967598 None:intergenic
AAATTCCTTTCATACATCTG+TGG 0.593600 2:+11967593 MsG0280007210.01.T01:five_prime_UTR
TGGAATCAGGTCTTACGCAA+AGG 0.604369 2:+11967738 MsG0280007210.01.T01:CDS
GTCATTGCTTAGATTGTACG+AGG 0.615162 2:-11967652 None:intergenic
TCAATATTATCCTTGTAAGG+AGG 0.618181 2:-11969016 None:intergenic
AGAAGCTCCATCTGATGTCA+AGG 0.618856 2:+11971105 MsG0280007210.01.T01:CDS
CTTACGCAAAGGTTGCAACT+GGG 0.624108 2:+11967749 MsG0280007210.01.T01:CDS
TCGAATGTGTGAGAAGAGTG+AGG 0.624817 2:-11967621 None:intergenic
TATAATATACCTTTCAGACC+GGG 0.625897 2:-11970780 None:intergenic
ACGAGCACGGTTCATTACAA+AGG 0.631640 2:-11967677 None:intergenic
AGGTTGTTGAAGAACGAGCA+CGG 0.636921 2:-11967690 None:intergenic
AAAGATGTCATTGATAGGAG+TGG 0.638393 2:-11967854 None:intergenic
TCCATATACTGTGAATGCAT+GGG 0.662253 2:+11969090 MsG0280007210.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGAACAAAAATAATAAA+AGG - Chr2:11967930-11967949 None:intergenic 10.0%
!! ATTGAACAAAAATAATAAAA+GGG - Chr2:11967929-11967948 None:intergenic 10.0%
!!! AAAACATTTTTTTATAACAA+AGG - Chr2:11968897-11968916 None:intergenic 10.0%
!!! ATTTTTGTTCAATTATTAAT+GGG + Chr2:11967936-11967955 MsG0280007210.01.T01:intron 10.0%
!!! TAAAAAAATGTTTTCTTTTT+CGG + Chr2:11968903-11968922 MsG0280007210.01.T01:intron 10.0%
!!! TATTTTTGTTCAATTATTAA+TGG + Chr2:11967935-11967954 MsG0280007210.01.T01:intron 10.0%
!! TAAGTTTAAAGATAATTAGA+AGG + Chr2:11970315-11970334 MsG0280007210.01.T01:intron 15.0%
!!! AAACTATTTTTAATTGAGTT+GGG + Chr2:11970206-11970225 MsG0280007210.01.T01:intron 15.0%
!!! TAAACTATTTTTAATTGAGT+TGG + Chr2:11970205-11970224 MsG0280007210.01.T01:intron 15.0%
!!! TAAGCAAAATCTTTAATTTA+AGG - Chr2:11968421-11968440 None:intergenic 15.0%
!! AAAAAGAAATAATGTAGTGA+AGG + Chr2:11970943-11970962 MsG0280007210.01.T01:intron 20.0%
!! AAAACAAATGTTTGTGTTAA+TGG - Chr2:11970831-11970850 None:intergenic 20.0%
!! AAAATAAATAGGAATAGGTT+TGG - Chr2:11969901-11969920 None:intergenic 20.0%
!! AAACAAATGTTTGTGTTAAT+GGG - Chr2:11970830-11970849 None:intergenic 20.0%
!! AAATAGAGAAAAAAATGAAG+TGG - Chr2:11967889-11967908 None:intergenic 20.0%
!! AATAGAGAAAAAAATGAAGT+GGG - Chr2:11967888-11967907 None:intergenic 20.0%
!! TAATATCATAAACCATTGTT+TGG - Chr2:11970974-11970993 None:intergenic 20.0%
!! TCAAATAAGTCAATTCAAAT+AGG + Chr2:11968871-11968890 MsG0280007210.01.T01:intron 20.0%
!! TGTATAAATAAGAGAATACA+AGG + Chr2:11969322-11969341 MsG0280007210.01.T01:intron 20.0%
!!! AAACCTATTCCTATTTATTT+TGG + Chr2:11969900-11969919 MsG0280007210.01.T01:intron 20.0%
!!! AGTTATTTTTCGATAAAAGA+AGG - Chr2:11970105-11970124 None:intergenic 20.0%
!!! TATGTTTTGATTATCTCTTA+GGG + Chr2:11968959-11968978 MsG0280007210.01.T01:intron 20.0%
!!! TTATGTTTTGATTATCTCTT+AGG + Chr2:11968958-11968977 MsG0280007210.01.T01:intron 20.0%
!!! TTTGTTAAGATTTTTTCTGT+TGG + Chr2:11971279-11971298 MsG0280007210.01.T01:three_prime_UTR 20.0%
! AAACCAACTTATAGTTTACA+TGG - Chr2:11968257-11968276 None:intergenic 25.0%
! AAATTGTCAAGTTAACTTAC+TGG - Chr2:11968021-11968040 None:intergenic 25.0%
! AATCAATAGCATGATGAATT+AGG + Chr2:11970612-11970631 MsG0280007210.01.T01:intron 25.0%
! AATTGTCAAGTTAACTTACT+GGG - Chr2:11968020-11968039 None:intergenic 25.0%
! ACTTATTTGAACTTATCTAC+CGG - Chr2:11968861-11968880 None:intergenic 25.0%
! AGATGTATGAAAGGAATTTA+TGG - Chr2:11967592-11967611 None:intergenic 25.0%
! AGTTAGTTGACATTGTTATA+GGG + Chr2:11969293-11969312 MsG0280007210.01.T01:intron 25.0%
! ATACTGTAATAGAGAGTTTA+TGG + Chr2:11968756-11968775 MsG0280007210.01.T01:intron 25.0%
! ATAGAGAAAAAAATGAAGTG+GGG - Chr2:11967887-11967906 None:intergenic 25.0%
! ATATAATATACCTTTCAGAC+CGG - Chr2:11970784-11970803 None:intergenic 25.0%
! ATTATTAATACTCTCCAAGA+TGG - Chr2:11968523-11968542 None:intergenic 25.0%
! CAATCTGGAAAAAGAAAAAA+AGG - Chr2:11970682-11970701 None:intergenic 25.0%
! GATCTAATTTCTGTAAACAT+TGG + Chr2:11969737-11969756 MsG0280007210.01.T01:intron 25.0%
! GCTTATAGCTTATATCAAAA+CGG - Chr2:11968488-11968507 None:intergenic 25.0%
! GGATAATATTGAGAAGTTTA+AGG + Chr2:11969027-11969046 MsG0280007210.01.T01:CDS 25.0%
! TAACTAACATCAATTGAATG+TGG + Chr2:11968733-11968752 MsG0280007210.01.T01:intron 25.0%
! TAGTTAGTTGACATTGTTAT+AGG + Chr2:11969292-11969311 MsG0280007210.01.T01:intron 25.0%
! TATATCCTAACAAAACACAA+AGG - Chr2:11969217-11969236 None:intergenic 25.0%
! TTCTATTGAAAGATTGAGAA+AGG - Chr2:11969452-11969471 None:intergenic 25.0%
! TTCTCAATATTATCCTTGTA+AGG - Chr2:11969022-11969041 None:intergenic 25.0%
! TTTCCATGTAAACTATAAGT+TGG + Chr2:11968251-11968270 MsG0280007210.01.T01:intron 25.0%
! TTTCTATCTTTCCATTGAAT+GGG - Chr2:11968569-11968588 None:intergenic 25.0%
! TTTCTTCAATTTGATGTTGT+AGG + Chr2:11967969-11967988 MsG0280007210.01.T01:intron 25.0%
!! ATTAAGTTGTTAATCCAAAC+AGG - Chr2:11968158-11968177 None:intergenic 25.0%
!! CGTTTTCTCTTTTTTCTTTT+TGG + Chr2:11969144-11969163 MsG0280007210.01.T01:intron 25.0%
!! TCTTTAATTTAAGGCTTGTT+TGG - Chr2:11968412-11968431 None:intergenic 25.0%
!! TTTTTCCAGATTGAGTTTTA+TGG + Chr2:11970689-11970708 MsG0280007210.01.T01:intron 25.0%
!!! AAACTATAAGTTGGTTTCAT+AGG + Chr2:11968260-11968279 MsG0280007210.01.T01:intron 25.0%
!!! TAAAAGAGTAGCTGTTATTT+TGG + Chr2:11971029-11971048 MsG0280007210.01.T01:intron 25.0%
!!! TAAATGCTTTATTTGCTGTT+TGG + Chr2:11969175-11969194 MsG0280007210.01.T01:intron 25.0%
AAATTCCTTTCATACATCTG+TGG + Chr2:11967593-11967612 MsG0280007210.01.T01:five_prime_UTR 30.0%
AACTAGATTAAATGCCAAAG+CGG + Chr2:11970888-11970907 MsG0280007210.01.T01:intron 30.0%
AAGGAAATGTGAATACCATA+AGG - Chr2:11970663-11970682 None:intergenic 30.0%
ACCTTAAAGATGTCATTGAT+AGG - Chr2:11967862-11967881 None:intergenic 30.0%
ACTATCTTAAAGTGGTGAAA+AGG - Chr2:11970448-11970467 None:intergenic 30.0%
ACTTCAAATTTGTCCCAAAA+TGG - Chr2:11971146-11971165 None:intergenic 30.0%
AGGTTATCTAGGAGAATTTA+TGG + Chr2:11968280-11968299 MsG0280007210.01.T01:intron 30.0%
ATAAATAGGAATAGGTTTGG+AGG - Chr2:11969898-11969917 None:intergenic 30.0%
ATCACTAATGCAAACATGTA+AGG - Chr2:11969540-11969559 None:intergenic 30.0%
ATGCTAGGACCAAAATAAAT+AGG - Chr2:11969912-11969931 None:intergenic 30.0%
ATTAAGCTTCAACAAAGGAT+CGG - Chr2:11969964-11969983 None:intergenic 30.0%
ATTTGGCTAGAAGAAATATG+TGG + Chr2:11970344-11970363 MsG0280007210.01.T01:intron 30.0%
CTATCTTAAAGTGGTGAAAA+GGG - Chr2:11970447-11970466 None:intergenic 30.0%
CTTTCTATCTTTCCATTGAA+TGG - Chr2:11968570-11968589 None:intergenic 30.0%
GAAGGAAATTACAAAAGACT+AGG - Chr2:11970087-11970106 None:intergenic 30.0%
GAAGTCAATCATAGAGATTA+AGG - Chr2:11969712-11969731 None:intergenic 30.0%
GAGAAAGGATAAAAGAGAAT+AGG - Chr2:11969437-11969456 None:intergenic 30.0%
GATAAGAAAGTTGTCATCTT+TGG + Chr2:11967991-11968010 MsG0280007210.01.T01:intron 30.0%
GGACCAAAATAAATAGGAAT+AGG - Chr2:11969906-11969925 None:intergenic 30.0%
TAATTAGAAGGCAAGAGATT+TGG + Chr2:11970327-11970346 MsG0280007210.01.T01:intron 30.0%
TATAATATACCTTTCAGACC+GGG - Chr2:11970783-11970802 None:intergenic 30.0%
TATAGTTAATGGCGTTAAAC+AGG - Chr2:11969875-11969894 None:intergenic 30.0%
TCAATATTATCCTTGTAAGG+AGG - Chr2:11969019-11969038 None:intergenic 30.0%
TCATCATAAGTGTTACAATG+AGG - Chr2:11969617-11969636 None:intergenic 30.0%
TCCTATCAATGACATCTTTA+AGG + Chr2:11967858-11967877 MsG0280007210.01.T01:CDS 30.0%
TTAATAATCAAAGAATCCCG+TGG + Chr2:11968535-11968554 MsG0280007210.01.T01:intron 30.0%
TTCAATTGATGTTAGTTAGC+AGG - Chr2:11968731-11968750 None:intergenic 30.0%
! AAAAACTATTTGCTGCCTTA+TGG + Chr2:11970645-11970664 MsG0280007210.01.T01:intron 30.0%
! AATTTAAGGCTTGTTTGGAT+TGG - Chr2:11968407-11968426 None:intergenic 30.0%
! ACATGTCCTTTGTGATTTTT+GGG + Chr2:11969646-11969665 MsG0280007210.01.T01:intron 30.0%
! ATAGAAAGCATAATGCTCTT+GGG + Chr2:11968582-11968601 MsG0280007210.01.T01:intron 30.0%
! ATTGAGAAGTTTAAGGCTAA+AGG + Chr2:11969034-11969053 MsG0280007210.01.T01:CDS 30.0%
! ATTTTTCCAAAGACACCAAA+TGG - Chr2:11969801-11969820 None:intergenic 30.0%
! CATGTAAGGAATCTTTCTTT+TGG - Chr2:11969526-11969545 None:intergenic 30.0%
! TACATGTCCTTTGTGATTTT+TGG + Chr2:11969645-11969664 MsG0280007210.01.T01:intron 30.0%
!! AGTTTTATGGAGACTTTGAT+GGG + Chr2:11970702-11970721 MsG0280007210.01.T01:CDS 30.0%
!! TGTTTCCTTTGTGTTTTGTT+AGG + Chr2:11969209-11969228 MsG0280007210.01.T01:intron 30.0%
!!! AACTGAAGACACTGATTTTA+AGG - Chr2:11967713-11967732 None:intergenic 30.0%
!!! GGAACGCATTTTTGAAATTA+AGG + Chr2:11969825-11969844 MsG0280007210.01.T01:intron 30.0%
AAAGATGTCATTGATAGGAG+TGG - Chr2:11967857-11967876 None:intergenic 35.0%
AAATATTCTATCAGCTCAGC+TGG - Chr2:11971262-11971281 None:intergenic 35.0%
AGCAGGTTAGAAAGTTAGTT+AGG - Chr2:11968714-11968733 None:intergenic 35.0%
AGCATTCCTTGAAAGAATAG+AGG + Chr2:11970498-11970517 MsG0280007210.01.T01:intron 35.0%
AGGTGTTTATGTCATTAGAC+AGG + Chr2:11968371-11968390 MsG0280007210.01.T01:intron 35.0%
AGTCTCCATAAAACTCAATC+TGG - Chr2:11970697-11970716 None:intergenic 35.0%
AGTTTCACAAGTGTTTATGC+CGG + Chr2:11968839-11968858 MsG0280007210.01.T01:intron 35.0%
ATCCATATACTGTGAATGCA+TGG + Chr2:11969089-11969108 MsG0280007210.01.T01:CDS 35.0%
CATTCCTTGAAAGAATAGAG+GGG + Chr2:11970500-11970519 MsG0280007210.01.T01:intron 35.0%
GAAAGAATAGAGGGGTTTAT+GGG + Chr2:11970508-11970527 MsG0280007210.01.T01:intron 35.0%
GAATTAACTACTGATCTCTC+TGG + Chr2:11970743-11970762 MsG0280007210.01.T01:CDS 35.0%
GCAGGTTAGAAAGTTAGTTA+GGG - Chr2:11968713-11968732 None:intergenic 35.0%
GCATTCCTTGAAAGAATAGA+GGG + Chr2:11970499-11970518 MsG0280007210.01.T01:intron 35.0%
GTCAATCATAGAGATTAAGG+CGG - Chr2:11969709-11969728 None:intergenic 35.0%
GTCCTATTTATAGAGCTAGA+TGG + Chr2:11968668-11968687 MsG0280007210.01.T01:intron 35.0%
TAAACCCCTCTATTCTTTCA+AGG - Chr2:11970507-11970526 None:intergenic 35.0%
TAGTTGAGATGCTAGAAACA+AGG - Chr2:11970167-11970186 None:intergenic 35.0%
TCCACATTAAGCTTCAACAA+AGG - Chr2:11969969-11969988 None:intergenic 35.0%
TCCATATACTGTGAATGCAT+GGG + Chr2:11969090-11969109 MsG0280007210.01.T01:CDS 35.0%
TCCTTTGTTGAAGCTTAATG+TGG + Chr2:11969965-11969984 MsG0280007210.01.T01:intron 35.0%
TCTGTAAACATTGGAACAGT+GGG + Chr2:11969746-11969765 MsG0280007210.01.T01:intron 35.0%
TGAAAGAATAGAGGGGTTTA+TGG + Chr2:11970507-11970526 MsG0280007210.01.T01:intron 35.0%
TTAAGCTTCAACAAAGGATC+GGG - Chr2:11969963-11969982 None:intergenic 35.0%
TTCTGTAAACATTGGAACAG+TGG + Chr2:11969745-11969764 MsG0280007210.01.T01:intron 35.0%
TTTCCAGCACTATCTTAAAG+TGG - Chr2:11970456-11970475 None:intergenic 35.0%
! ACTCAATTCTGCACAGTTTT+GGG + Chr2:11969486-11969505 MsG0280007210.01.T01:intron 35.0%
! AGGTTTGGAGGTATAGTTAA+TGG - Chr2:11969886-11969905 None:intergenic 35.0%
! GATAGAAAGCATAATGCTCT+TGG + Chr2:11968581-11968600 MsG0280007210.01.T01:intron 35.0%
! GTTGGTTTCATAGGTTATCT+AGG + Chr2:11968269-11968288 MsG0280007210.01.T01:intron 35.0%
! TACTCAATTCTGCACAGTTT+TGG + Chr2:11969485-11969504 MsG0280007210.01.T01:intron 35.0%
! TGATTCTGTTATTTGCGTGT+CGG + Chr2:11969060-11969079 MsG0280007210.01.T01:CDS 35.0%
!! AAAACCACTGCTTTAGAACA+AGG - Chr2:11968934-11968953 None:intergenic 35.0%
!! AAACTATTTGAGAGCGCTTA+TGG - Chr2:11968824-11968843 None:intergenic 35.0%
!! CTCCATCTAGCTCTATAAAT+AGG - Chr2:11968673-11968692 None:intergenic 35.0%
!! GAGTTTTATGGAGACTTTGA+TGG + Chr2:11970701-11970720 MsG0280007210.01.T01:CDS 35.0%
!! GCTGTTTTCATTAACCATCT+TGG + Chr2:11968506-11968525 MsG0280007210.01.T01:intron 35.0%
!! GTTTCTAGCATCTCAACTAA+AGG + Chr2:11970168-11970187 MsG0280007210.01.T01:intron 35.0%
!!! AGCTGTTATTTTGGTGTAGA+TGG + Chr2:11971038-11971057 MsG0280007210.01.T01:intron 35.0%
!!! TTATTTTGGTCCTAGCATGA+GGG + Chr2:11969914-11969933 MsG0280007210.01.T01:intron 35.0%
!!! TTTATTTTGGTCCTAGCATG+AGG + Chr2:11969913-11969932 MsG0280007210.01.T01:intron 35.0%
AAAATGTACCTGATGCTTCC+AGG + Chr2:11971233-11971252 MsG0280007210.01.T01:three_prime_UTR 40.0%
AAAGCTCGTAATTGGGATGA+AGG + Chr2:11967811-11967830 MsG0280007210.01.T01:CDS 40.0%
AAATGCGCATTAAGCTCAGA+GGG - Chr2:11970054-11970073 None:intergenic 40.0%
AGAGACCACAGATGTATGAA+AGG - Chr2:11967601-11967620 None:intergenic 40.0%
AGAGATTAAGGCGGAAGAAT+TGG - Chr2:11969700-11969719 None:intergenic 40.0%
AGCATATGTGGTAGATGGAA+AGG + Chr2:11971063-11971082 MsG0280007210.01.T01:CDS 40.0%
AGTAGCGACTCTATTGTGAA+GGG + Chr2:11969386-11969405 MsG0280007210.01.T01:intron 40.0%
ATCACAAAGGACATGTACAC+TGG - Chr2:11969642-11969661 None:intergenic 40.0%
ATTTGTAGTTCCCTCATGCT+AGG - Chr2:11969927-11969946 None:intergenic 40.0%
CAAACATGTTCCTCCTTACA+AGG + Chr2:11969006-11969025 MsG0280007210.01.T01:CDS 40.0%
CTAATTGCCCTACCTTCTTT+GGG + Chr2:11969771-11969790 MsG0280007210.01.T01:intron 40.0%
CTTCAGTTCTTCATGGAATC+AGG + Chr2:11967725-11967744 MsG0280007210.01.T01:CDS 40.0%
GAAAACGCTTACAGCATCTT+TGG - Chr2:11969131-11969150 None:intergenic 40.0%
GACCTTCATTAGAAGAAGAG+AGG - Chr2:11970013-11970032 None:intergenic 40.0%
GATAGGAGTGGTAGAGAATT+TGG - Chr2:11967845-11967864 None:intergenic 40.0%
GTCATTGCTTAGATTGTACG+AGG - Chr2:11967655-11967674 None:intergenic 40.0%
GTGAAGGAATCACCAAACAA+TGG + Chr2:11970959-11970978 MsG0280007210.01.T01:intron 40.0%
TATTGTGAAGGGGAAAACCT+TGG + Chr2:11969397-11969416 MsG0280007210.01.T01:intron 40.0%
TCAGTGTCTTCAGTTCTTCA+TGG + Chr2:11967718-11967737 MsG0280007210.01.T01:CDS 40.0%
TCTCCAGAAAGCTCGTAATT+GGG + Chr2:11967804-11967823 MsG0280007210.01.T01:CDS 40.0%
TGAGGGAACTACAAATTTCC+TGG + Chr2:11969931-11969950 MsG0280007210.01.T01:intron 40.0%
TGTGGTAGATGGAAAGGTTA+AGG + Chr2:11971069-11971088 MsG0280007210.01.T01:CDS 40.0%
TGTTGCAATTGTGTCCTGTT+TGG + Chr2:11968141-11968160 MsG0280007210.01.T01:intron 40.0%
! AAAAAGTGACTTTGCCGCTT+TGG - Chr2:11970905-11970924 None:intergenic 40.0%
! AGCTTTCTGGAGAGAAACAT+TGG - Chr2:11967797-11967816 None:intergenic 40.0%
! GGTGTCTTTGGAAAAATCAC+AGG + Chr2:11969804-11969823 MsG0280007210.01.T01:intron 40.0%
! TCTGGTGCAGAAACCATTTT+GGG + Chr2:11971130-11971149 MsG0280007210.01.T01:CDS 40.0%
! TTCTGGTGCAGAAACCATTT+TGG + Chr2:11971129-11971148 MsG0280007210.01.T01:CDS 40.0%
!! TCACCACTTTAAGATAGTGC+TGG + Chr2:11970450-11970469 MsG0280007210.01.T01:intron 40.0%
ACGAGCACGGTTCATTACAA+AGG - Chr2:11967680-11967699 None:intergenic 45.0%
AGAAGCTCCATCTGATGTCA+AGG + Chr2:11971105-11971124 MsG0280007210.01.T01:CDS 45.0%
AGGTTGTTGAAGAACGAGCA+CGG - Chr2:11967693-11967712 None:intergenic 45.0%
ATCTCTTAGGGTGCATACAC+AGG + Chr2:11968971-11968990 MsG0280007210.01.T01:intron 45.0%
ATGGCAATCCCAAAGAAGGT+AGG - Chr2:11969782-11969801 None:intergenic 45.0%
CAAATGCGCATTAAGCTCAG+AGG - Chr2:11970055-11970074 None:intergenic 45.0%
CATCCCAATTACGAGCTTTC+TGG - Chr2:11967810-11967829 None:intergenic 45.0%
CCAAAGAAGGTAGGGCAATT+AGG - Chr2:11969773-11969792 None:intergenic 45.0%
CCAAATGGCAATCCCAAAGA+AGG - Chr2:11969786-11969805 None:intergenic 45.0%
CCAGAAACCTTGACATCAGA+TGG - Chr2:11971115-11971134 None:intergenic 45.0%
CCATCTGATGTCAAGGTTTC+TGG + Chr2:11971112-11971131 MsG0280007210.01.T01:CDS 45.0%
CCTAATTGCCCTACCTTCTT+TGG + Chr2:11969770-11969789 MsG0280007210.01.T01:intron 45.0%
CGTTAAACAGGCTGAGGATT+TGG - Chr2:11969863-11969882 None:intergenic 45.0%
CTCTCCAGAAAGCTCGTAAT+TGG + Chr2:11967803-11967822 MsG0280007210.01.T01:CDS 45.0%
CTTACGCAAAGGTTGCAACT+GGG + Chr2:11967749-11967768 MsG0280007210.01.T01:CDS 45.0%
GAGTAGCGACTCTATTGTGA+AGG + Chr2:11969385-11969404 MsG0280007210.01.T01:intron 45.0%
GATAGACCTCATAACTCAGC+AGG + Chr2:11969987-11970006 MsG0280007210.01.T01:intron 45.0%
GCCCATGCATTCACAGTATA+TGG - Chr2:11969094-11969113 None:intergenic 45.0%
GGCCTCTCTTCTTCTAATGA+AGG + Chr2:11970008-11970027 MsG0280007210.01.T01:intron 45.0%
GTAGCGACTCTATTGTGAAG+GGG + Chr2:11969387-11969406 MsG0280007210.01.T01:intron 45.0%
GTGTAGATGGTCAGCATATG+TGG + Chr2:11971051-11971070 MsG0280007210.01.T01:intron 45.0%
GTTCTCTCAATCAGTCTCAC+AGG + Chr2:11968351-11968370 MsG0280007210.01.T01:intron 45.0%
TAATGGCGTTAAACAGGCTG+AGG - Chr2:11969869-11969888 None:intergenic 45.0%
TCAAAGAATCCCGTGGATCT+AGG + Chr2:11968542-11968561 MsG0280007210.01.T01:intron 45.0%
TCGAATGTGTGAGAAGAGTG+AGG - Chr2:11967624-11967643 None:intergenic 45.0%
TCTTACGCAAAGGTTGCAAC+TGG + Chr2:11967748-11967767 MsG0280007210.01.T01:CDS 45.0%
TGGAATCAGGTCTTACGCAA+AGG + Chr2:11967738-11967757 MsG0280007210.01.T01:CDS 45.0%
TGGATCTAGGTCCCATTCAA+TGG + Chr2:11968555-11968574 MsG0280007210.01.T01:intron 45.0%
TGGCAATCCCAAAGAAGGTA+GGG - Chr2:11969781-11969800 None:intergenic 45.0%
TGGGAGCTTTCACAAAAGCT+TGG + Chr2:11970721-11970740 MsG0280007210.01.T01:CDS 45.0%
TGGTCAGCATATGTGGTAGA+TGG + Chr2:11971058-11971077 MsG0280007210.01.T01:CDS 45.0%
TTGAATGGGACCTAGATCCA+CGG - Chr2:11968555-11968574 None:intergenic 45.0%
!! CCTTCTTTGGGATTGCCATT+TGG + Chr2:11969783-11969802 MsG0280007210.01.T01:intron 45.0%
!! GGATTGCCATTTGGTGTCTT+TGG + Chr2:11969792-11969811 MsG0280007210.01.T01:intron 45.0%
!! GTAGCCTTGTTCTAAAGCAG+TGG + Chr2:11968927-11968946 MsG0280007210.01.T01:intron 45.0%
!! TGTGTTAATGGGCTCAGTCT+TGG - Chr2:11970819-11970838 None:intergenic 45.0%
!!! GCGGCAAAGTCACTTTTTCA+CGG + Chr2:11970907-11970926 MsG0280007210.01.T01:intron 45.0%
AGCTTCAACAAAGGATCGGG+AGG - Chr2:11969960-11969979 None:intergenic 50.0%
AGGAGAGAACCTATCCTCCA+AGG - Chr2:11969417-11969436 None:intergenic 50.0%
AGGGGAAAACCTTGGAGGAT+AGG + Chr2:11969405-11969424 MsG0280007210.01.T01:intron 50.0%
ATGGGCTCAGTCTTGGTTCA+AGG - Chr2:11970812-11970831 None:intergenic 50.0%
GCTTCAACAAAGGATCGGGA+GGG - Chr2:11969959-11969978 None:intergenic 50.0%
GGGGGTCCCAAAAATCACAA+AGG - Chr2:11969655-11969674 None:intergenic 50.0%
TGAATGGGACCTAGATCCAC+GGG - Chr2:11968554-11968573 None:intergenic 50.0%
TGTGAAGGGGAAAACCTTGG+AGG + Chr2:11969400-11969419 MsG0280007210.01.T01:intron 50.0%
!!! TTTTTGGGACCCCCTTGTTG+TGG + Chr2:11969661-11969680 MsG0280007210.01.T01:intron 50.0%
CGCATTAAGCTCAGAGGGAG+GGG - Chr2:11970049-11970068 None:intergenic 55.0%
GAGAGGCCTGCTGAGTTATG+AGG - Chr2:11969996-11970015 None:intergenic 55.0%
GATCTCTCTGGTGCTCTGCT+TGG + Chr2:11970755-11970774 MsG0280007210.01.T01:CDS 55.0%
GCGCATTAAGCTCAGAGGGA+GGG - Chr2:11970050-11970069 None:intergenic 55.0%
TATCAGCTCAGCTGGAAGCC+TGG - Chr2:11971254-11971273 None:intergenic 55.0%
TGCGCATTAAGCTCAGAGGG+AGG - Chr2:11970051-11970070 None:intergenic 55.0%
TGCTTGGAACCCGGTCTGAA+AGG + Chr2:11970771-11970790 MsG0280007210.01.T01:CDS 55.0%
! ACCCCCTTGTTGTGGAGGAT+CGG + Chr2:11969669-11969688 MsG0280007210.01.T01:intron 55.0%
ACCGCCGATCCTCCACAACA+AGG - Chr2:11969676-11969695 None:intergenic 60.0%
CAAAGGATCGGGAGGGTTCC+AGG - Chr2:11969952-11969971 None:intergenic 60.0%
CCGCCGATCCTCCACAACAA+GGG - Chr2:11969675-11969694 None:intergenic 60.0%
CGCCGATCCTCCACAACAAG+GGG - Chr2:11969674-11969693 None:intergenic 60.0%
CTGGTGCTCTGCTTGGAACC+CGG + Chr2:11970762-11970781 MsG0280007210.01.T01:CDS 60.0%
GCCGATCCTCCACAACAAGG+GGG - Chr2:11969673-11969692 None:intergenic 60.0%
! CCCTTGTTGTGGAGGATCGG+CGG + Chr2:11969672-11969691 MsG0280007210.01.T01:intron 60.0%
! TTGGGACCCCCTTGTTGTGG+AGG + Chr2:11969664-11969683 MsG0280007210.01.T01:intron 60.0%
!! GCTGGAAGCCTGGAAGCATC+AGG - Chr2:11971244-11971263 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 11967571 11971303 11967571 ID=MsG0280007210.01;Name=MsG0280007210.01
Chr2 mRNA 11967571 11971303 11967571 ID=MsG0280007210.01.T01;Parent=MsG0280007210.01;Name=MsG0280007210.01.T01;_AED=0.42;_eAED=0.42;_QI=99|0.66|1|1|1|1|4|141|189
Chr2 exon 11967571 11967879 11967571 ID=MsG0280007210.01.T01:exon:2785;Parent=MsG0280007210.01.T01
Chr2 exon 11968980 11969138 11968980 ID=MsG0280007210.01.T01:exon:2786;Parent=MsG0280007210.01.T01
Chr2 exon 11970698 11970792 11970698 ID=MsG0280007210.01.T01:exon:2787;Parent=MsG0280007210.01.T01
Chr2 exon 11971057 11971303 11971057 ID=MsG0280007210.01.T01:exon:2788;Parent=MsG0280007210.01.T01
Chr2 five_prime_UTR 11967571 11967669 11967571 ID=MsG0280007210.01.T01:five_prime_utr;Parent=MsG0280007210.01.T01
Chr2 CDS 11967670 11967879 11967670 ID=MsG0280007210.01.T01:cds;Parent=MsG0280007210.01.T01
Chr2 CDS 11968980 11969138 11968980 ID=MsG0280007210.01.T01:cds;Parent=MsG0280007210.01.T01
Chr2 CDS 11970698 11970792 11970698 ID=MsG0280007210.01.T01:cds;Parent=MsG0280007210.01.T01
Chr2 CDS 11971057 11971162 11971057 ID=MsG0280007210.01.T01:cds;Parent=MsG0280007210.01.T01
Chr2 three_prime_UTR 11971163 11971303 11971163 ID=MsG0280007210.01.T01:three_prime_utr;Parent=MsG0280007210.01.T01
Gene Sequence

>MsG0280007210.01.T01

ATGACAACCTTTGTAATGAACCGTGCTCGTTCTTCAACAACCTTAAAATCAGTGTCTTCAGTTCTTCATGGAATCAGGTCTTACGCAAAGGTTGCAACTGGGTCTGACATAATCTCTGCTGCATCCAATGTTTCTCTCCAGAAAGCTCGTAATTGGGATGAAGGTGTTTCTTCCAAATTCTCTACCACTCCTATCAATGACATCTTTAAGGGTGCATACACAGGAGTTTGTTCAAGCAAACATGTTCCTCCTTACAAGGATAATATTGAGAAGTTTAAGGCTAAAGGAGTTGATTCTGTTATTTGCGTGTCGGTTAATGATCCATATACTGTGAATGCATGGGCTGAGAAGCTTCAAGCCAAAGATGCTATTGAGTTTTATGGAGACTTTGATGGGAGCTTTCACAAAAGCTTGGAATTAACTACTGATCTCTCTGGTGCTCTGCTTGGAACCCGGTCTGAAAGATGGTCAGCATATGTGGTAGATGGAAAGGTTAAGGCTCTTAACGTCGAAGAAGCTCCATCTGATGTCAAGGTTTCTGGTGCAGAAACCATTTTGGGACAAATTTGA
Protein sequence

>MsG0280007210.01.T01

MTTFVMNRARSSTTLKSVSSVLHGIRSYAKVATGSDIISAASNVSLQKARNWDEGVSSKFSTTPINDIFKGAYTGVCSSKHVPPYKDNIEKFKAKGVDSVICVSVNDPYTVNAWAEKLQAKDAIEFYGDFDGSFHKSLELTTDLSGALLGTRSERWSAYVVDGKVKALNVEEAPSDVKVSGAETILGQI*