Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007510.01.T01 | XP_013462945.1 | 96.988 | 166 | 1 | 1 | 1 | 162 | 1 | 166 | 7.72E-113 | 327 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007510.01.T01 | O23310 | 82.645 | 121 | 13 | 1 | 1 | 113 | 1 | 121 | 1.78E-68 | 207 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007510.01.T01 | I3TAX1 | 96.988 | 166 | 1 | 1 | 1 | 162 | 1 | 166 | 3.69e-113 | 327 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0280007510.01.T01 | TF | NF-YB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007510.01 | MsG0580024035.01 | 0.831950 | 1.248375e-55 | 1.375522e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007510.01.T01 | MTR_2g026710 | 96.988 | 166 | 1 | 1 | 1 | 162 | 1 | 166 | 9.35e-117 | 327 |
| MsG0280007510.01.T01 | MTR_5g095740 | 66.061 | 165 | 39 | 4 | 1 | 151 | 1 | 162 | 5.71e-68 | 204 |
| MsG0280007510.01.T01 | MTR_3g058980 | 72.535 | 142 | 20 | 3 | 1 | 128 | 1 | 137 | 6.77e-66 | 199 |
| MsG0280007510.01.T01 | MTR_8g091720 | 74.046 | 131 | 27 | 2 | 7 | 130 | 22 | 152 | 2.34e-64 | 196 |
| MsG0280007510.01.T01 | MTR_7g061270 | 64.789 | 142 | 43 | 2 | 9 | 143 | 22 | 163 | 8.26e-62 | 188 |
| MsG0280007510.01.T01 | MTR_7g100650 | 84.694 | 98 | 15 | 0 | 9 | 106 | 22 | 119 | 3.89e-61 | 187 |
| MsG0280007510.01.T01 | MTR_1g072790 | 67.969 | 128 | 35 | 1 | 8 | 129 | 24 | 151 | 5.44e-61 | 186 |
| MsG0280007510.01.T01 | MTR_4g133938 | 72.308 | 130 | 27 | 3 | 1 | 121 | 1 | 130 | 3.68e-60 | 183 |
| MsG0280007510.01.T01 | MTR_8g093920 | 77.778 | 108 | 22 | 1 | 12 | 117 | 39 | 146 | 4.53e-58 | 180 |
| MsG0280007510.01.T01 | MTR_4g133952 | 53.947 | 152 | 54 | 4 | 2 | 140 | 48 | 196 | 1.00e-53 | 170 |
| MsG0280007510.01.T01 | MTR_0392s0020 | 68.269 | 104 | 33 | 0 | 4 | 107 | 9 | 112 | 3.60e-52 | 163 |
| MsG0280007510.01.T01 | MTR_1g088860 | 69.524 | 105 | 32 | 0 | 3 | 107 | 26 | 130 | 8.61e-52 | 164 |
| MsG0280007510.01.T01 | MTR_4g119500 | 61.864 | 118 | 44 | 1 | 4 | 120 | 12 | 129 | 1.09e-50 | 159 |
| MsG0280007510.01.T01 | MTR_1g039040 | 63.725 | 102 | 37 | 0 | 9 | 110 | 2 | 103 | 3.05e-48 | 154 |
| MsG0280007510.01.T01 | MTR_1g083070 | 64.130 | 92 | 33 | 0 | 13 | 104 | 4 | 95 | 8.60e-41 | 134 |
| MsG0280007510.01.T01 | MTR_1g029070 | 54.839 | 93 | 42 | 0 | 18 | 110 | 57 | 149 | 1.10e-30 | 109 |
| MsG0280007510.01.T01 | MTR_5g095900 | 44.762 | 105 | 55 | 1 | 1 | 102 | 1 | 105 | 1.56e-23 | 91.3 |
| MsG0280007510.01.T01 | MTR_1g028480 | 46.429 | 84 | 44 | 1 | 43 | 125 | 19 | 102 | 1.77e-19 | 79.0 |
| MsG0280007510.01.T01 | MTR_1g029100 | 40.789 | 76 | 45 | 0 | 18 | 93 | 7 | 82 | 1.22e-18 | 76.3 |
| MsG0280007510.01.T01 | MTR_2g056000 | 32.500 | 80 | 54 | 0 | 14 | 93 | 42 | 121 | 2.33e-14 | 67.4 |
| MsG0280007510.01.T01 | MTR_4g112380 | 32.530 | 83 | 56 | 0 | 9 | 91 | 7 | 89 | 3.15e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007510.01.T01 | AT4G14540 | 82.645 | 121 | 13 | 1 | 1 | 113 | 1 | 121 | 1.81e-69 | 207 |
| MsG0280007510.01.T01 | AT5G47640 | 83.929 | 112 | 16 | 2 | 12 | 123 | 26 | 135 | 4.28e-64 | 195 |
| MsG0280007510.01.T01 | AT2G38880 | 71.429 | 126 | 32 | 2 | 7 | 131 | 14 | 136 | 4.19e-60 | 183 |
| MsG0280007510.01.T01 | AT2G38880 | 71.429 | 126 | 32 | 2 | 7 | 131 | 14 | 136 | 5.33e-60 | 183 |
| MsG0280007510.01.T01 | AT2G38880 | 71.429 | 126 | 32 | 2 | 7 | 131 | 14 | 136 | 5.33e-60 | 183 |
| MsG0280007510.01.T01 | AT2G38880 | 71.429 | 126 | 32 | 2 | 7 | 131 | 14 | 136 | 5.33e-60 | 183 |
| MsG0280007510.01.T01 | AT2G38880 | 70.635 | 126 | 31 | 2 | 7 | 131 | 14 | 134 | 2.74e-58 | 178 |
| MsG0280007510.01.T01 | AT3G53340 | 62.252 | 151 | 40 | 4 | 8 | 143 | 24 | 172 | 3.05e-58 | 179 |
| MsG0280007510.01.T01 | AT3G53340 | 62.252 | 151 | 40 | 4 | 8 | 143 | 24 | 172 | 3.05e-58 | 179 |
| MsG0280007510.01.T01 | AT2G37060 | 80.808 | 99 | 19 | 0 | 8 | 106 | 25 | 123 | 3.49e-58 | 179 |
| MsG0280007510.01.T01 | AT2G37060 | 80.808 | 99 | 19 | 0 | 8 | 106 | 25 | 123 | 3.49e-58 | 179 |
| MsG0280007510.01.T01 | AT2G37060 | 80.808 | 99 | 19 | 0 | 8 | 106 | 25 | 123 | 3.49e-58 | 179 |
| MsG0280007510.01.T01 | AT2G13570 | 83.333 | 96 | 16 | 0 | 12 | 107 | 35 | 130 | 1.17e-57 | 179 |
| MsG0280007510.01.T01 | AT2G38880 | 82.653 | 98 | 16 | 1 | 7 | 103 | 14 | 111 | 8.58e-57 | 174 |
| MsG0280007510.01.T01 | AT2G38880 | 82.653 | 98 | 16 | 1 | 7 | 103 | 14 | 111 | 1.80e-56 | 174 |
| MsG0280007510.01.T01 | AT2G38880 | 82.653 | 98 | 16 | 1 | 7 | 103 | 14 | 111 | 1.80e-56 | 174 |
| MsG0280007510.01.T01 | AT2G38880 | 84.783 | 92 | 14 | 0 | 12 | 103 | 20 | 111 | 2.51e-56 | 174 |
| MsG0280007510.01.T01 | AT2G38880 | 84.783 | 92 | 14 | 0 | 12 | 103 | 20 | 111 | 2.51e-56 | 174 |
| MsG0280007510.01.T01 | AT2G38880 | 84.783 | 92 | 14 | 0 | 12 | 103 | 20 | 111 | 2.51e-56 | 174 |
| MsG0280007510.01.T01 | AT3G53340 | 75.728 | 103 | 25 | 0 | 8 | 110 | 24 | 126 | 2.71e-56 | 174 |
| MsG0280007510.01.T01 | AT3G53340 | 75.728 | 103 | 25 | 0 | 8 | 110 | 24 | 126 | 2.71e-56 | 174 |
| MsG0280007510.01.T01 | AT3G53340 | 78.125 | 96 | 21 | 0 | 8 | 103 | 24 | 119 | 1.28e-55 | 171 |
| MsG0280007510.01.T01 | AT2G47810 | 71.028 | 107 | 31 | 0 | 7 | 113 | 45 | 151 | 1.25e-52 | 165 |
| MsG0280007510.01.T01 | AT5G47670 | 66.337 | 101 | 34 | 0 | 7 | 107 | 23 | 123 | 3.37e-50 | 160 |
| MsG0280007510.01.T01 | AT5G47670 | 66.337 | 101 | 34 | 0 | 7 | 107 | 52 | 152 | 6.20e-50 | 160 |
| MsG0280007510.01.T01 | AT5G47670 | 66.337 | 101 | 34 | 0 | 7 | 107 | 52 | 152 | 6.20e-50 | 160 |
| MsG0280007510.01.T01 | AT1G21970 | 48.366 | 153 | 68 | 3 | 11 | 152 | 57 | 209 | 7.54e-47 | 152 |
| MsG0280007510.01.T01 | AT1G09030 | 61.458 | 96 | 37 | 0 | 13 | 108 | 3 | 98 | 4.17e-43 | 140 |
| MsG0280007510.01.T01 | AT5G08190 | 34.286 | 105 | 63 | 2 | 9 | 109 | 7 | 109 | 1.97e-17 | 75.1 |
| MsG0280007510.01.T01 | AT5G23090 | 32.381 | 105 | 65 | 2 | 9 | 109 | 7 | 109 | 9.28e-17 | 73.2 |
| MsG0280007510.01.T01 | AT5G08190 | 37.349 | 83 | 52 | 0 | 9 | 91 | 7 | 89 | 1.89e-16 | 72.4 |
| MsG0280007510.01.T01 | AT5G23090 | 34.940 | 83 | 54 | 0 | 9 | 91 | 7 | 89 | 7.26e-16 | 70.9 |
| MsG0280007510.01.T01 | AT5G23090 | 34.940 | 83 | 54 | 0 | 9 | 91 | 7 | 89 | 7.26e-16 | 70.9 |
| MsG0280007510.01.T01 | AT5G23090 | 34.940 | 83 | 54 | 0 | 9 | 91 | 7 | 89 | 7.26e-16 | 70.9 |
| MsG0280007510.01.T01 | AT5G23090 | 32.911 | 79 | 47 | 2 | 30 | 104 | 15 | 91 | 2.39e-11 | 58.9 |
Find 36 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGGTAGTGGTGGAGTTTA+TGG | 0.223086 | 2:+16438881 | MsG0280007510.01.T01:CDS |
| TCAACGGTGATGATCTTCTT+TGG | 0.252720 | 2:+16438729 | MsG0280007510.01.T01:CDS |
| TTTGAGATCTTTGCGTTTGC+TGG | 0.274169 | 2:-16438608 | None:intergenic |
| ATCCTACTCACGTTTGCTAT+TGG | 0.294063 | 2:-16438569 | None:intergenic |
| GGGAGTGGATCGCCATCTTC+TGG | 0.311375 | 2:+16438965 | MsG0280007510.01.T01:CDS |
| CGTGAGTAGGATCATGAAAA+AGG | 0.331544 | 2:+16438580 | MsG0280007510.01.T01:CDS |
| GGACATAATATGTATGGATC+TGG | 0.358939 | 2:+16438944 | MsG0280007510.01.T01:CDS |
| GAGTTCATCAGTTTCATAAC+AGG | 0.366706 | 2:+16438665 | MsG0280007510.01.T01:CDS |
| CTTTGGGCTATGACAACTCT+TGG | 0.376889 | 2:+16438746 | MsG0280007510.01.T01:CDS |
| CAACGGTGATGATCTTCTTT+GGG | 0.389396 | 2:+16438730 | MsG0280007510.01.T01:CDS |
| CAGGTTGCAGAGAGCAAGAT+AGG | 0.404952 | 2:+16438540 | MsG0280007510.01.T01:CDS |
| GGAGAGAAGAGTGCTATGAT+TGG | 0.411557 | 2:+16438827 | MsG0280007510.01.T01:CDS |
| AGTGGTGGAGTTTATGGTCA+TGG | 0.452362 | 2:+16438887 | MsG0280007510.01.T01:CDS |
| GGAGTTTATGGTCATGGAGT+TGG | 0.458217 | 2:+16438893 | MsG0280007510.01.T01:CDS |
| CGAGTACTCCTTCCAGAAGA+TGG | 0.478915 | 2:-16438977 | None:intergenic |
| AGAAGAGTGCTATGATTGGT+AGG | 0.483869 | 2:+16438831 | MsG0280007510.01.T01:CDS |
| ATCATCATCATCATAGAAGA+TGG | 0.485382 | 2:-16438920 | None:intergenic |
| ATCTTCTATGATGATGATGA+TGG | 0.489435 | 2:+16438922 | MsG0280007510.01.T01:CDS |
| GGTAGGAGTGATCAGAGAGA+TGG | 0.493822 | 2:+16438848 | MsG0280007510.01.T01:CDS |
| GGAAATGTTGGTGAGGGTAG+TGG | 0.495110 | 2:+16438869 | MsG0280007510.01.T01:CDS |
| TTTGAGTAAGTATAGAGAGA+TGG | 0.496858 | 2:+16438802 | MsG0280007510.01.T01:CDS |
| ATGATGGGACATAATATGTA+TGG | 0.500614 | 2:+16438938 | MsG0280007510.01.T01:CDS |
| GATCAGAGAGATGGAAATGT+TGG | 0.512395 | 2:+16438857 | MsG0280007510.01.T01:CDS |
| GACATAATATGTATGGATCT+GGG | 0.545156 | 2:+16438945 | MsG0280007510.01.T01:CDS |
| AGAGATGGAAATGTTGGTGA+GGG | 0.549342 | 2:+16438863 | MsG0280007510.01.T01:CDS |
| GAATCAGACAATGAGTCAGC+AGG | 0.557061 | 2:+16438521 | MsG0280007510.01.T01:CDS |
| ATAAGTGTCAGAAAGAGAAG+AGG | 0.567471 | 2:+16438699 | MsG0280007510.01.T01:CDS |
| AGTAAGTATAGAGAGATGGA+AGG | 0.568189 | 2:+16438806 | MsG0280007510.01.T01:CDS |
| AATGTTGGTGAGGGTAGTGG+TGG | 0.579830 | 2:+16438872 | MsG0280007510.01.T01:CDS |
| AATATGTATGGATCTGGGAG+TGG | 0.582558 | 2:+16438950 | MsG0280007510.01.T01:CDS |
| TTCCAATAGCAAACGTGAGT+AGG | 0.595976 | 2:+16438567 | MsG0280007510.01.T01:CDS |
| TTACTCAAATAAATCTTGAG+TGG | 0.610816 | 2:-16438788 | None:intergenic |
| GAGAAGAGGAAAACAATCAA+CGG | 0.615125 | 2:+16438713 | MsG0280007510.01.T01:CDS |
| GAGAGATGGAAATGTTGGTG+AGG | 0.620088 | 2:+16438862 | MsG0280007510.01.T01:CDS |
| GTGGATCGCCATCTTCTGGA+AGG | 0.622809 | 2:+16438969 | MsG0280007510.01.T01:CDS |
| TCTTCTATGATGATGATGAT+GGG | 0.641124 | 2:+16438923 | MsG0280007510.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTACTCAAATAAATCTTGAG+TGG | - | Chr2:16438791-16438810 | None:intergenic | 25.0% |
| ATCATCATCATCATAGAAGA+TGG | - | Chr2:16438923-16438942 | None:intergenic | 30.0% | |
| GACATAATATGTATGGATCT+GGG | + | Chr2:16438945-16438964 | MsG0280007510.01.T01:CDS | 30.0% | |
| TTTGAGTAAGTATAGAGAGA+TGG | + | Chr2:16438802-16438821 | MsG0280007510.01.T01:CDS | 30.0% | |
| ! | ATCTTCTATGATGATGATGA+TGG | + | Chr2:16438922-16438941 | MsG0280007510.01.T01:CDS | 30.0% |
| ! | ATGATGGGACATAATATGTA+TGG | + | Chr2:16438938-16438957 | MsG0280007510.01.T01:CDS | 30.0% |
| ! | TCTTCTATGATGATGATGAT+GGG | + | Chr2:16438923-16438942 | MsG0280007510.01.T01:CDS | 30.0% |
| AGTAAGTATAGAGAGATGGA+AGG | + | Chr2:16438806-16438825 | MsG0280007510.01.T01:CDS | 35.0% | |
| ATAAGTGTCAGAAAGAGAAG+AGG | + | Chr2:16438699-16438718 | MsG0280007510.01.T01:CDS | 35.0% | |
| GAGAAGAGGAAAACAATCAA+CGG | + | Chr2:16438713-16438732 | MsG0280007510.01.T01:CDS | 35.0% | |
| GAGTTCATCAGTTTCATAAC+AGG | + | Chr2:16438665-16438684 | MsG0280007510.01.T01:CDS | 35.0% | |
| GGACATAATATGTATGGATC+TGG | + | Chr2:16438944-16438963 | MsG0280007510.01.T01:CDS | 35.0% | |
| AATATGTATGGATCTGGGAG+TGG | + | Chr2:16438950-16438969 | MsG0280007510.01.T01:CDS | 40.0% | |
| CAACGGTGATGATCTTCTTT+GGG | + | Chr2:16438730-16438749 | MsG0280007510.01.T01:CDS | 40.0% | |
| CGTGAGTAGGATCATGAAAA+AGG | + | Chr2:16438580-16438599 | MsG0280007510.01.T01:CDS | 40.0% | |
| GATCAGAGAGATGGAAATGT+TGG | + | Chr2:16438857-16438876 | MsG0280007510.01.T01:CDS | 40.0% | |
| TCAACGGTGATGATCTTCTT+TGG | + | Chr2:16438729-16438748 | MsG0280007510.01.T01:CDS | 40.0% | |
| TTCCAATAGCAAACGTGAGT+AGG | + | Chr2:16438567-16438586 | MsG0280007510.01.T01:CDS | 40.0% | |
| ! | AGAGATGGAAATGTTGGTGA+GGG | + | Chr2:16438863-16438882 | MsG0280007510.01.T01:CDS | 40.0% |
| ! | TTTGAGATCTTTGCGTTTGC+TGG | - | Chr2:16438611-16438630 | None:intergenic | 40.0% |
| !! | AGAAGAGTGCTATGATTGGT+AGG | + | Chr2:16438831-16438850 | MsG0280007510.01.T01:CDS | 40.0% |
| !! | ATCCTACTCACGTTTGCTAT+TGG | - | Chr2:16438572-16438591 | None:intergenic | 40.0% |
| CTTTGGGCTATGACAACTCT+TGG | + | Chr2:16438746-16438765 | MsG0280007510.01.T01:CDS | 45.0% | |
| GAATCAGACAATGAGTCAGC+AGG | + | Chr2:16438521-16438540 | MsG0280007510.01.T01:CDS | 45.0% | |
| GAGAGATGGAAATGTTGGTG+AGG | + | Chr2:16438862-16438881 | MsG0280007510.01.T01:CDS | 45.0% | |
| GGAGTTTATGGTCATGGAGT+TGG | + | Chr2:16438893-16438912 | MsG0280007510.01.T01:CDS | 45.0% | |
| ! | AGTGGTGGAGTTTATGGTCA+TGG | + | Chr2:16438887-16438906 | MsG0280007510.01.T01:CDS | 45.0% |
| !! | GGAGAGAAGAGTGCTATGAT+TGG | + | Chr2:16438827-16438846 | MsG0280007510.01.T01:CDS | 45.0% |
| CAGGTTGCAGAGAGCAAGAT+AGG | + | Chr2:16438540-16438559 | MsG0280007510.01.T01:CDS | 50.0% | |
| CGAGTACTCCTTCCAGAAGA+TGG | - | Chr2:16438980-16438999 | None:intergenic | 50.0% | |
| GGTAGGAGTGATCAGAGAGA+TGG | + | Chr2:16438848-16438867 | MsG0280007510.01.T01:CDS | 50.0% | |
| ! | AATGTTGGTGAGGGTAGTGG+TGG | + | Chr2:16438872-16438891 | MsG0280007510.01.T01:CDS | 50.0% |
| ! | GAGGGTAGTGGTGGAGTTTA+TGG | + | Chr2:16438881-16438900 | MsG0280007510.01.T01:CDS | 50.0% |
| ! | GGAAATGTTGGTGAGGGTAG+TGG | + | Chr2:16438869-16438888 | MsG0280007510.01.T01:CDS | 50.0% |
| GTGGATCGCCATCTTCTGGA+AGG | + | Chr2:16438969-16438988 | MsG0280007510.01.T01:CDS | 55.0% | |
| GGGAGTGGATCGCCATCTTC+TGG | + | Chr2:16438965-16438984 | MsG0280007510.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 16438515 | 16439003 | 16438515 | ID=MsG0280007510.01;Name=MsG0280007510.01 |
| Chr2 | mRNA | 16438515 | 16439003 | 16438515 | ID=MsG0280007510.01.T01;Parent=MsG0280007510.01;Name=MsG0280007510.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|162 |
| Chr2 | exon | 16438515 | 16439003 | 16438515 | ID=MsG0280007510.01.T01:exon:1233;Parent=MsG0280007510.01.T01 |
| Chr2 | CDS | 16438515 | 16439003 | 16438515 | ID=MsG0280007510.01.T01:cds;Parent=MsG0280007510.01.T01 |
| Gene Sequence |
| Protein sequence |
