AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007538.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007538.01.T01 MTR_2g027390 96.759 432 10 2 1 428 1 432 0.0 842
MsG0280007538.01.T01 MTR_7g017500 39.474 114 66 3 93 205 149 260 1.63e-22 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007538.01.T01 AT5G47790 58.508 429 116 8 1 428 1 368 6.95e-162 461
MsG0280007538.01.T01 AT5G38840 39.640 111 64 3 96 205 93 201 3.86e-19 90.5

Find 145 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTAATGGCTCTATTAAA+AGG 0.121567 2:-16873701 None:intergenic
AAAGGGCAACTTACCATTTA+AGG 0.131774 2:-16873683 None:intergenic
CAGTGTATATGTAATTGATT+TGG 0.192768 2:+16872682 MsG0280007538.01.T01:intron
TTGGAACCGTTCTCTGTTAA+TGG 0.193902 2:+16871878 MsG0280007538.01.T01:CDS
AACCTATGCAGATTGCATTT+TGG 0.226234 2:+16873499 MsG0280007538.01.T01:three_prime_UTR
GGTGCTAGATAGGATTAATT+TGG 0.279458 2:+16872177 MsG0280007538.01.T01:CDS
ATTGCAGGTTCATCCTTAAA+TGG 0.280176 2:+16873670 MsG0280007538.01.T01:three_prime_UTR
AGAAGATGAGAGTTTCATTC+AGG 0.291478 2:+16873044 MsG0280007538.01.T01:CDS
AGTGGAGGCGGCTCCGTTGT+TGG 0.292771 2:+16872039 MsG0280007538.01.T01:CDS
GTGGAGGCGGCTCCGTTGTT+GGG 0.315269 2:+16872040 MsG0280007538.01.T01:CDS
AAGAGCATGTTCTTAAGATT+TGG 0.324829 2:+16871727 MsG0280007538.01.T01:five_prime_UTR
GTTTAAGAAAGCCGAGAGTT+TGG 0.325196 2:+16871859 MsG0280007538.01.T01:CDS
GTGAGAGAATCGTACCGATT+AGG 0.340181 2:-16871345 None:intergenic
TGAGAGAATCGTACCGATTA+GGG 0.340359 2:-16871344 None:intergenic
CACAAACTCTTAAAGATTGC+AGG 0.342390 2:+16873655 MsG0280007538.01.T01:three_prime_UTR
ACTACCAAGCTCAGTGGATT+TGG 0.351010 2:-16872966 None:intergenic
CTGCAATCTTTAAGAGTTTG+TGG 0.355617 2:-16873654 None:intergenic
GGGATTTACGATGACCTATA+TGG 0.356648 2:+16873313 MsG0280007538.01.T01:CDS
GAGGACTGCCCTTTCCCCTT+TGG 0.357778 2:-16873205 None:intergenic
TTTCATTCAGGGATCAGGTT+GGG 0.361537 2:+16873056 MsG0280007538.01.T01:CDS
AGTGTATATGTAATTGATTT+GGG 0.362845 2:+16872683 MsG0280007538.01.T01:intron
GAGGATGATGATGATTTGTT+TGG 0.370753 2:+16873466 MsG0280007538.01.T01:CDS
CCCTCGTCCTTCACCACCCA+CGG 0.373388 2:+16872841 MsG0280007538.01.T01:CDS
CTTCCTTCCTTAACACCAAC+TGG 0.386824 2:-16873139 None:intergenic
GAAGAACATCTGGTCTTGAT+AGG 0.392084 2:+16871837 MsG0280007538.01.T01:CDS
ACCTGGTCCAGTTGGTGTTA+AGG 0.396500 2:+16873132 MsG0280007538.01.T01:CDS
TTCATTCAGGGATCAGGTTG+GGG 0.397217 2:+16873057 MsG0280007538.01.T01:CDS
CGCGGCTCAATCGCCCAATC+TGG 0.397896 2:-16872115 None:intergenic
ACAGTGGTAAACATTAATTA+TGG 0.405465 2:-16873626 None:intergenic
TGTAATTTGAAGAGAATGTA+TGG 0.407152 2:+16871815 MsG0280007538.01.T01:exon
GGAGAGTTAGTTGAGGTTGT+TGG 0.408959 2:+16873079 MsG0280007538.01.T01:CDS
TAAGGTAGTTGGTCATTCTT+CGG 0.414880 2:+16871937 MsG0280007538.01.T01:CDS
TCTTAGCAGCTCTTTCTGAT+TGG 0.422101 2:-16873015 None:intergenic
TCCGATGGGTCAGGAGGTGG+TGG 0.423621 2:-16872875 None:intergenic
TCTCCATTCTCCTTAGCTGC+TGG 0.430224 2:-16873403 None:intergenic
GTTGTTGGGGCAGAATCAAC+AGG 0.433225 2:+16872054 MsG0280007538.01.T01:CDS
GAGAGTTTCATTCAGGGATC+AGG 0.440722 2:+16873051 MsG0280007538.01.T01:CDS
AGGATGGTCAGGTGCTAGAT+AGG 0.444081 2:+16872167 MsG0280007538.01.T01:CDS
GATGTGGCAGCCACCAGATT+GGG 0.444291 2:+16872102 MsG0280007538.01.T01:CDS
AATACGCTGCTGAATCGTTA+TGG 0.447922 2:+16872920 MsG0280007538.01.T01:CDS
CAAGCACAAAATCACAAGTC+TGG 0.448208 2:-16872231 None:intergenic
GCCTCTTCATCCGATGGGTC+AGG 0.453710 2:-16872884 None:intergenic
AGAATGTATGGAAGAACATC+TGG 0.453804 2:+16871827 MsG0280007538.01.T01:exon
ATTTAATGGCTCTATTAAAA+GGG 0.454822 2:-16873700 None:intergenic
AGCGTTTCTAAAACCCTAAT+CGG 0.460272 2:+16871331 MsG0280007538.01.T01:five_prime_UTR
CTGTAATTCCAAAGGGGAAA+GGG 0.464451 2:+16873197 MsG0280007538.01.T01:CDS
CAAACTACTGTAATTCCAAA+GGG 0.471274 2:+16873190 MsG0280007538.01.T01:CDS
AAACTCAACAGGTGAATCCT+TGG 0.474394 2:-16872744 None:intergenic
ACTATGAGCTCATAAACAAC+TGG 0.476196 2:-16871771 None:intergenic
CGATGTGGCAGCCACCAGAT+TGG 0.476461 2:+16872101 MsG0280007538.01.T01:CDS
TCCTTAACACCAACTGGACC+AGG 0.478092 2:-16873133 None:intergenic
GAAGCGTAGTAGAGAGAAAG+AGG 0.478426 2:-16871298 None:intergenic
GCGCCAGCAGCTAAGGAGAA+TGG 0.478653 2:+16873400 MsG0280007538.01.T01:CDS
CCTCGTCCTTCACCACCCAC+GGG 0.483891 2:+16872842 MsG0280007538.01.T01:CDS
TGTCCCACTTCAAACTCAAC+AGG 0.484199 2:-16872755 None:intergenic
CTGTCAAAGTTGGATCAGCC+TGG 0.486875 2:+16873347 MsG0280007538.01.T01:CDS
TTGATCCCCGTGGGTGGTGA+AGG 0.486979 2:-16872848 None:intergenic
AGGATGATGATGATTTGTTT+GGG 0.488049 2:+16873467 MsG0280007538.01.T01:CDS
TCCTCGTAATCAGCAATGCT+TGG 0.489223 2:-16873448 None:intergenic
GAATCATTATCTGTCAAAGT+TGG 0.489351 2:+16873337 MsG0280007538.01.T01:CDS
TGTACAGAATTCTTCGACTG+TGG 0.489814 2:-16871971 None:intergenic
CAGATAATGATTCTCCATAT+AGG 0.494590 2:-16873327 None:intergenic
GGAAGTTATGAAGGATGGTC+AGG 0.495075 2:+16872156 MsG0280007538.01.T01:CDS
TGGAGGCGGCTCCGTTGTTG+GGG 0.498085 2:+16872041 MsG0280007538.01.T01:CDS
AATCAACAGGCAACTCAGAT+TGG 0.499106 2:+16872067 MsG0280007538.01.T01:CDS
TTGGGCGATTGAGCCGCGAC+CGG 0.501004 2:+16872120 MsG0280007538.01.T01:CDS
GGAAAGGGCAGTCCTCTGCT+AGG 0.502686 2:+16873212 MsG0280007538.01.T01:CDS
TACTTGGAAGTTATGAAGGA+TGG 0.503632 2:+16872151 MsG0280007538.01.T01:CDS
GAGGTAGATTCATCCCAAAA+AGG 0.506021 2:+16873235 MsG0280007538.01.T01:CDS
TTCTCAGAAAGGTGTGGGAG+TGG 0.506673 2:+16872021 MsG0280007538.01.T01:CDS
TGTTGCTCAGTCTTCTAGTA+AGG 0.506725 2:+16871919 MsG0280007538.01.T01:CDS
GAAGATGAGAGTTTCATTCA+GGG 0.508142 2:+16873045 MsG0280007538.01.T01:CDS
GAAAGGTGTGGGAGTGGAGG+CGG 0.508188 2:+16872027 MsG0280007538.01.T01:CDS
GTTAAGGAAGGAAGTCTAGT+TGG 0.508857 2:+16873148 MsG0280007538.01.T01:CDS
TGGGCGATTGAGCCGCGACC+GGG 0.511260 2:+16872121 MsG0280007538.01.T01:CDS
AACATTAATTATGGACGCAA+TGG 0.516800 2:-16873617 None:intergenic
TAGTTGGTCATTCTTCGGCT+TGG 0.516977 2:+16871942 MsG0280007538.01.T01:CDS
TGAACGAGCGCCAGCAGCTA+AGG 0.523991 2:+16873393 MsG0280007538.01.T01:CDS
CATCAACATGGTTCTCAGAA+AGG 0.526028 2:+16872010 MsG0280007538.01.T01:CDS
TCATCCGATGGGTCAGGAGG+TGG 0.527608 2:-16872878 None:intergenic
CAGTCTTCTAGTAAGGTAGT+TGG 0.528464 2:+16871926 MsG0280007538.01.T01:CDS
GCTAAGGAGAATGGAGAAAG+TGG 0.536680 2:+16873409 MsG0280007538.01.T01:CDS
CTCTCGCCAACATGCTGCGG+TGG 0.540409 2:+16872267 MsG0280007538.01.T01:CDS
GGCTCAATCGCCCAATCTGG+TGG 0.540495 2:-16872112 None:intergenic
CCCGTGGGTGGTGAAGGACG+AGG 0.543398 2:-16872842 None:intergenic
TCCTGACCCATCGGATGAAG+AGG 0.544867 2:+16872883 MsG0280007538.01.T01:CDS
TCTTAATATGTATAATCTCG+TGG 0.545734 2:-16872798 None:intergenic
GAGCTTGGTAGTGCAACAAA+TGG 0.548432 2:+16872977 MsG0280007538.01.T01:CDS
CCCACCACCTCCTGACCCAT+CGG 0.549380 2:+16872874 MsG0280007538.01.T01:CDS
AAGCGTAGTAGAGAGAAAGA+GGG 0.551110 2:-16871297 None:intergenic
GTTTCATTCAGGGATCAGGT+TGG 0.560524 2:+16873055 MsG0280007538.01.T01:CDS
GAAAGGGCAGTCCTCTGCTA+GGG 0.561223 2:+16873213 MsG0280007538.01.T01:CDS
GCTCGTTCACCACTACTGAC+AGG 0.562972 2:-16873379 None:intergenic
CAACAGGCAACTCAGATTGG+TGG 0.563642 2:+16872070 MsG0280007538.01.T01:CDS
CAAACGCTCATTTGCAACAA+AGG 0.565573 2:-16872720 None:intergenic
TTCACCTGTTGAGTTTGAAG+TGG 0.567231 2:+16872751 MsG0280007538.01.T01:CDS
ACAACAGCCTCTTCATCCGA+TGG 0.568113 2:-16872890 None:intergenic
GGGAAATTGATCCCCGTGGG+TGG 0.570676 2:-16872854 None:intergenic
TCACCTGTTGAGTTTGAAGT+GGG 0.574165 2:+16872752 MsG0280007538.01.T01:CDS
AGAGTTTGTGGTGTATACAG+TGG 0.575232 2:-16873642 None:intergenic
GAGGTTGTTGGAATTTCAGA+TGG 0.576473 2:+16873091 MsG0280007538.01.T01:CDS
CAGGCAACTCAGATTGGTGG+AGG 0.577255 2:+16872073 MsG0280007538.01.T01:CDS
CATGGTTCTCAGAAAGGTGT+GGG 0.580915 2:+16872016 MsG0280007538.01.T01:CDS
TGTCTCTCGCCAACATGCTG+CGG 0.581927 2:+16872264 MsG0280007538.01.T01:CDS
ACAAACTACTGTAATTCCAA+AGG 0.585279 2:+16873189 MsG0280007538.01.T01:CDS
GAATTACCATTAACAGAGAA+CGG 0.587204 2:-16871884 None:intergenic
CTTAAGATTTGGTGTCATTG+TGG 0.587507 2:+16871738 MsG0280007538.01.T01:five_prime_UTR
TCTAACCTTCCGTTCTTGTG+AGG 0.588526 2:-16872292 None:intergenic
ACTGTAATTCCAAAGGGGAA+AGG 0.588891 2:+16873196 MsG0280007538.01.T01:CDS
AGAGAACGGTTCCAAACTCT+CGG 0.589404 2:-16871870 None:intergenic
TGTCAAAGTTGGATCAGCCT+GGG 0.595464 2:+16873348 MsG0280007538.01.T01:CDS
TTTAAGGTTTGCGCTAAGAG+AGG 0.598765 2:+16871702 MsG0280007538.01.T01:intron
AGGGCAGTCCTCTGCTAGGG+AGG 0.602420 2:+16873216 MsG0280007538.01.T01:CDS
GAGGAACCACCGCAGCATGT+TGG 0.602912 2:-16872273 None:intergenic
TGGTGGGAAATTGATCCCCG+TGG 0.605958 2:-16872858 None:intergenic
TCAGAAAGGTGTGGGAGTGG+AGG 0.607307 2:+16872024 MsG0280007538.01.T01:CDS
TGCAAATGAGCGTTTGACCA+AGG 0.607826 2:+16872727 MsG0280007538.01.T01:CDS
GGTCCAGTTGGTGTTAAGGA+AGG 0.608986 2:+16873136 MsG0280007538.01.T01:CDS
GAAACAGAACCTGGTCCAGT+TGG 0.609238 2:+16873124 MsG0280007538.01.T01:CDS
ACATGGTTCTCAGAAAGGTG+TGG 0.612081 2:+16872015 MsG0280007538.01.T01:CDS
GTGTCCAAATCCACTGAGCT+TGG 0.614575 2:+16872962 MsG0280007538.01.T01:CDS
GCGGTGGTTCCTCACAAGAA+CGG 0.615525 2:+16872283 MsG0280007538.01.T01:CDS
ACTGACAGGTGAGTATGCCC+AGG 0.616123 2:-16873365 None:intergenic
CAACAGCCTCTTCATCCGAT+GGG 0.619090 2:-16872889 None:intergenic
TCATTCAGGGATCAGGTTGG+GGG 0.620615 2:+16873058 MsG0280007538.01.T01:CDS
TGTTGATTCTGCCCCAACAA+CGG 0.623282 2:-16872052 None:intergenic
GCATACTCACCTGTCAGTAG+TGG 0.625393 2:+16873370 MsG0280007538.01.T01:CDS
TGAATCTACCTCCCTAGCAG+AGG 0.627223 2:-16873224 None:intergenic
CCGTGGGTGGTGAAGGACGA+GGG 0.627786 2:-16872841 None:intergenic
TTCCAAAATGCAATCTGCAT+AGG 0.630025 2:-16873501 None:intergenic
TCCAAGCATTGCTGATTACG+AGG 0.635210 2:+16873447 MsG0280007538.01.T01:CDS
TTGGTGGAGGGCAGTCGATG+TGG 0.637100 2:+16872086 MsG0280007538.01.T01:CDS
ACAGAATTCTTCGACTGTGG+AGG 0.638226 2:-16871968 None:intergenic
TCTTCATCCGATGGGTCAGG+AGG 0.642327 2:-16872881 None:intergenic
TGGTTCCTCACAAGAACGGA+AGG 0.642477 2:+16872287 MsG0280007538.01.T01:CDS
GGTGGGAAATTGATCCCCGT+GGG 0.643525 2:-16872857 None:intergenic
CCGATGGGTCAGGAGGTGGT+GGG 0.653058 2:-16872874 None:intergenic
TGTTGCACTACCAAGCTCAG+TGG 0.656951 2:-16872972 None:intergenic
TTGAAGTGGGACAATCATTG+CGG 0.662047 2:+16872765 MsG0280007538.01.T01:CDS
ATTGATTTGGGATCTGCACA+TGG 0.667462 2:+16872695 MsG0280007538.01.T01:CDS
CTCGTCCTTCACCACCCACG+GGG 0.671541 2:+16872843 MsG0280007538.01.T01:CDS
AGGCAACTCAGATTGGTGGA+GGG 0.676455 2:+16872074 MsG0280007538.01.T01:CDS
TCACAGAATCAACATCAACA+TGG 0.707104 2:+16871998 MsG0280007538.01.T01:CDS
AAACTACTGTAATTCCAAAG+GGG 0.709535 2:+16873191 MsG0280007538.01.T01:CDS
GTAGATGTAGAAACAGAACC+TGG 0.729040 2:+16873115 MsG0280007538.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATGTCTTATATTATTT+AGG + Chr2:16872608-16872627 MsG0280007538.01.T01:intron 10.0%
!!! AAAAAAAAAGATGAATTTTT+TGG - Chr2:16871402-16871421 None:intergenic 10.0%
!!! ACATAATTTTTAAGTAAGTA+AGG - Chr2:16871655-16871674 None:intergenic 15.0%
!!! ATGTATTGATTATTTTCTTT+TGG + Chr2:16871585-16871604 MsG0280007538.01.T01:intron 15.0%
!!! ATTATTTAGAATATGCAATT+CGG - Chr2:16872390-16872409 None:intergenic 15.0%
!!! TGTATTGATTATTTTCTTTT+GGG + Chr2:16871586-16871605 MsG0280007538.01.T01:intron 15.0%
!!! TTTTTGTCAAATTGATTTTA+AGG + Chr2:16871686-16871705 MsG0280007538.01.T01:intron 15.0%
!! AAATGAATTACAAATGACTT+AGG + Chr2:16872546-16872565 MsG0280007538.01.T01:intron 20.0%
!! ATAATAATTAGAAGCCAAAT+GGG - Chr2:16871567-16871586 None:intergenic 20.0%
!!! AGTGTATATGTAATTGATTT+GGG + Chr2:16872683-16872702 MsG0280007538.01.T01:intron 20.0%
!!! ATAGACGAAGAAATATTTTT+GGG + Chr2:16872200-16872219 MsG0280007538.01.T01:CDS 20.0%
! ACAGTGGTAAACATTAATTA+TGG - Chr2:16873629-16873648 None:intergenic 25.0%
! ATTACAACTATAAGTTGCAA+GGG - Chr2:16872644-16872663 None:intergenic 25.0%
! CAGTGTATATGTAATTGATT+TGG + Chr2:16872682-16872701 MsG0280007538.01.T01:intron 25.0%
! CATAATAATTAGAAGCCAAA+TGG - Chr2:16871568-16871587 None:intergenic 25.0%
! TATTACAACTATAAGTTGCA+AGG - Chr2:16872645-16872664 None:intergenic 25.0%
! TCTACCCTAGAATTAAAATT+GGG - Chr2:16871517-16871536 None:intergenic 25.0%
! TCTTAATATGTATAATCTCG+TGG - Chr2:16872801-16872820 None:intergenic 25.0%
! TGTAATTTGAAGAGAATGTA+TGG + Chr2:16871815-16871834 MsG0280007538.01.T01:exon 25.0%
! TTCTACCCTAGAATTAAAAT+TGG - Chr2:16871518-16871537 None:intergenic 25.0%
! TTTCGTTTGATAAGGTAATA+TGG - Chr2:16872485-16872504 None:intergenic 25.0%
!! AAATCATTTGCTTGCATTTT+TGG + Chr2:16871538-16871557 MsG0280007538.01.T01:intron 25.0%
!! GATAGACGAAGAAATATTTT+TGG + Chr2:16872199-16872218 MsG0280007538.01.T01:CDS 25.0%
!! TTTTTACTTGGAAGTTATGA+AGG + Chr2:16872147-16872166 MsG0280007538.01.T01:CDS 25.0%
AAACTACTGTAATTCCAAAG+GGG + Chr2:16873191-16873210 MsG0280007538.01.T01:CDS 30.0%
AACATTAATTATGGACGCAA+TGG - Chr2:16873620-16873639 None:intergenic 30.0%
ACAAACTACTGTAATTCCAA+AGG + Chr2:16873189-16873208 MsG0280007538.01.T01:CDS 30.0%
CAAACTACTGTAATTCCAAA+GGG + Chr2:16873190-16873209 MsG0280007538.01.T01:CDS 30.0%
CAGATAATGATTCTCCATAT+AGG - Chr2:16873330-16873349 None:intergenic 30.0%
CTACCCTAGAATTAAAATTG+GGG - Chr2:16871516-16871535 None:intergenic 30.0%
GAATCATTATCTGTCAAAGT+TGG + Chr2:16873337-16873356 MsG0280007538.01.T01:CDS 30.0%
GAATTACCATTAACAGAGAA+CGG - Chr2:16871887-16871906 None:intergenic 30.0%
TACCCTAGAATTAAAATTGG+GGG - Chr2:16871515-16871534 None:intergenic 30.0%
TTGAATGCTTTCGTTTGATA+AGG - Chr2:16872493-16872512 None:intergenic 30.0%
! AAGAGCATGTTCTTAAGATT+TGG + Chr2:16871727-16871746 MsG0280007538.01.T01:five_prime_UTR 30.0%
! TTTATCAGTCACACCTTTTT+GGG - Chr2:16873251-16873270 None:intergenic 30.0%
!! AATATGGTGTCATTTGATAC+AGG - Chr2:16872469-16872488 None:intergenic 30.0%
!! AGGATGATGATGATTTGTTT+GGG + Chr2:16873467-16873486 MsG0280007538.01.T01:CDS 30.0%
!!! TACCCCCAATTTTAATTCTA+GGG + Chr2:16871510-16871529 MsG0280007538.01.T01:intron 30.0%
!!! TGCATTTTGGAATAGATTTG+TGG + Chr2:16873512-16873531 MsG0280007538.01.T01:three_prime_UTR 30.0%
!!! TTACCCCCAATTTTAATTCT+AGG + Chr2:16871509-16871528 MsG0280007538.01.T01:intron 30.0%
AAAGGGCAACTTACCATTTA+AGG - Chr2:16873686-16873705 None:intergenic 35.0%
ACTATGAGCTCATAAACAAC+TGG - Chr2:16871774-16871793 None:intergenic 35.0%
ACTTCCAAGTAAAAAACACC+CGG - Chr2:16872142-16872161 None:intergenic 35.0%
AGAAGATGAGAGTTTCATTC+AGG + Chr2:16873044-16873063 MsG0280007538.01.T01:CDS 35.0%
AGAATGTATGGAAGAACATC+TGG + Chr2:16871827-16871846 MsG0280007538.01.T01:exon 35.0%
AGGTAATAGATGCAAGCTAT+TGG - Chr2:16872449-16872468 None:intergenic 35.0%
ATCCATTCCAGTAACATCAA+AGG - Chr2:16872332-16872351 None:intergenic 35.0%
ATTGCAGGTTCATCCTTAAA+TGG + Chr2:16873670-16873689 MsG0280007538.01.T01:three_prime_UTR 35.0%
CACAAACTCTTAAAGATTGC+AGG + Chr2:16873655-16873674 MsG0280007538.01.T01:three_prime_UTR 35.0%
GAAGATGAGAGTTTCATTCA+GGG + Chr2:16873045-16873064 MsG0280007538.01.T01:CDS 35.0%
GGTGCTAGATAGGATTAATT+TGG + Chr2:16872177-16872196 MsG0280007538.01.T01:CDS 35.0%
TAAGGTAGTTGGTCATTCTT+CGG + Chr2:16871937-16871956 MsG0280007538.01.T01:CDS 35.0%
TACTTGGAAGTTATGAAGGA+TGG + Chr2:16872151-16872170 MsG0280007538.01.T01:CDS 35.0%
TCACAGAATCAACATCAACA+TGG + Chr2:16871998-16872017 MsG0280007538.01.T01:CDS 35.0%
TGCAACAAATGGCAAAAACA+AGG + Chr2:16872988-16873007 MsG0280007538.01.T01:CDS 35.0%
TTCCAAAATGCAATCTGCAT+AGG - Chr2:16873504-16873523 None:intergenic 35.0%
! AACCTATGCAGATTGCATTT+TGG + Chr2:16873499-16873518 MsG0280007538.01.T01:three_prime_UTR 35.0%
! CTGCAATCTTTAAGAGTTTG+TGG - Chr2:16873657-16873676 None:intergenic 35.0%
! GTAAGTTGCCCTTTTAATAG+AGG + Chr2:16873692-16873711 MsG0280007538.01.T01:three_prime_UTR 35.0%
! GTTTATCAGTCACACCTTTT+TGG - Chr2:16873252-16873271 None:intergenic 35.0%
!! AAAAGCTAAAAGTGCTGAAG+TGG - Chr2:16872574-16872593 None:intergenic 35.0%
!! AGCGTTTCTAAAACCCTAAT+CGG + Chr2:16871331-16871350 MsG0280007538.01.T01:five_prime_UTR 35.0%
!! ATCGTAAATCCCAGTTTTAG+CGG - Chr2:16873305-16873324 None:intergenic 35.0%
!! ATTTTGAGTATGACACCACA+GGG + Chr2:16873536-16873555 MsG0280007538.01.T01:three_prime_UTR 35.0%
!! CTTAAGATTTGGTGTCATTG+TGG + Chr2:16871738-16871757 MsG0280007538.01.T01:five_prime_UTR 35.0%
!! GAGGATGATGATGATTTGTT+TGG + Chr2:16873466-16873485 MsG0280007538.01.T01:CDS 35.0%
!! TATTTTGAGTATGACACCAC+AGG + Chr2:16873535-16873554 MsG0280007538.01.T01:three_prime_UTR 35.0%
!!! GACGAAGAAATATTTTTGGG+AGG + Chr2:16872203-16872222 MsG0280007538.01.T01:CDS 35.0%
!!! TTGTGCGCTTTTTTATCATC+TGG + Chr2:16873584-16873603 MsG0280007538.01.T01:three_prime_UTR 35.0%
AAACTCAACAGGTGAATCCT+TGG - Chr2:16872747-16872766 None:intergenic 40.0%
AAGCGTAGTAGAGAGAAAGA+GGG - Chr2:16871300-16871319 None:intergenic 40.0%
AATACGCTGCTGAATCGTTA+TGG + Chr2:16872920-16872939 MsG0280007538.01.T01:CDS 40.0%
AATCAACAGGCAACTCAGAT+TGG + Chr2:16872067-16872086 MsG0280007538.01.T01:CDS 40.0%
ACTGTAATTCCAAAGGGGAA+AGG + Chr2:16873196-16873215 MsG0280007538.01.T01:CDS 40.0%
AGAGTTTGTGGTGTATACAG+TGG - Chr2:16873645-16873664 None:intergenic 40.0%
ATCAAAAACCCCGCTAAAAC+TGG + Chr2:16873292-16873311 MsG0280007538.01.T01:CDS 40.0%
CAAACGCTCATTTGCAACAA+AGG - Chr2:16872723-16872742 None:intergenic 40.0%
CAAGCACAAAATCACAAGTC+TGG - Chr2:16872234-16872253 None:intergenic 40.0%
CAGTCTTCTAGTAAGGTAGT+TGG + Chr2:16871926-16871945 MsG0280007538.01.T01:CDS 40.0%
CATCAACATGGTTCTCAGAA+AGG + Chr2:16872010-16872029 MsG0280007538.01.T01:CDS 40.0%
CTCCTTTGATGTTACTGGAA+TGG + Chr2:16872327-16872346 MsG0280007538.01.T01:intron 40.0%
CTGTAATTCCAAAGGGGAAA+GGG + Chr2:16873197-16873216 MsG0280007538.01.T01:CDS 40.0%
GAGGTAGATTCATCCCAAAA+AGG + Chr2:16873235-16873254 MsG0280007538.01.T01:CDS 40.0%
GAGGTTGTTGGAATTTCAGA+TGG + Chr2:16873091-16873110 MsG0280007538.01.T01:CDS 40.0%
GGGATTTACGATGACCTATA+TGG + Chr2:16873313-16873332 MsG0280007538.01.T01:CDS 40.0%
GTAGATGTAGAAACAGAACC+TGG + Chr2:16873115-16873134 MsG0280007538.01.T01:CDS 40.0%
GTTAAGGAAGGAAGTCTAGT+TGG + Chr2:16873148-16873167 MsG0280007538.01.T01:CDS 40.0%
GTTTAAGAAAGCCGAGAGTT+TGG + Chr2:16871859-16871878 MsG0280007538.01.T01:CDS 40.0%
TCAAAAACCCCGCTAAAACT+GGG + Chr2:16873293-16873312 MsG0280007538.01.T01:CDS 40.0%
TCTCTCTCCTTTGATGTTAC+TGG + Chr2:16872322-16872341 MsG0280007538.01.T01:intron 40.0%
TCTTAGCAGCTCTTTCTGAT+TGG - Chr2:16873018-16873037 None:intergenic 40.0%
TGTACAGAATTCTTCGACTG+TGG - Chr2:16871974-16871993 None:intergenic 40.0%
TGTTGCTCAGTCTTCTAGTA+AGG + Chr2:16871919-16871938 MsG0280007538.01.T01:CDS 40.0%
TTCACCTGTTGAGTTTGAAG+TGG + Chr2:16872751-16872770 MsG0280007538.01.T01:CDS 40.0%
TTGAAGTGGGACAATCATTG+CGG + Chr2:16872765-16872784 MsG0280007538.01.T01:CDS 40.0%
TTGGAACCGTTCTCTGTTAA+TGG + Chr2:16871878-16871897 MsG0280007538.01.T01:CDS 40.0%
TTTCATTCAGGGATCAGGTT+GGG + Chr2:16873056-16873075 MsG0280007538.01.T01:CDS 40.0%
! TCACCTGTTGAGTTTGAAGT+GGG + Chr2:16872752-16872771 MsG0280007538.01.T01:CDS 40.0%
! TTTAAGGTTTGCGCTAAGAG+AGG + Chr2:16871702-16871721 MsG0280007538.01.T01:intron 40.0%
!! ATTGATTTGGGATCTGCACA+TGG + Chr2:16872695-16872714 MsG0280007538.01.T01:CDS 40.0%
!! CGGGGTTTTTGATCTTGTTT+AGG - Chr2:16873285-16873304 None:intergenic 40.0%
!! GAAGAACATCTGGTCTTGAT+AGG + Chr2:16871837-16871856 MsG0280007538.01.T01:CDS 40.0%
!! TCGTAAATCCCAGTTTTAGC+GGG - Chr2:16873304-16873323 None:intergenic 40.0%
!! TGAGAGAATCGTACCGATTA+GGG - Chr2:16871347-16871366 None:intergenic 40.0%
!!! TGCATTTTTGGTGACCCATT+TGG + Chr2:16871550-16871569 MsG0280007538.01.T01:intron 40.0%
AAGTAAAAAACACCCGGTCG+CGG - Chr2:16872136-16872155 None:intergenic 45.0%
ACAGAATTCTTCGACTGTGG+AGG - Chr2:16871971-16871990 None:intergenic 45.0%
ACATGGTTCTCAGAAAGGTG+TGG + Chr2:16872015-16872034 MsG0280007538.01.T01:CDS 45.0%
AGAGAACGGTTCCAAACTCT+CGG - Chr2:16871873-16871892 None:intergenic 45.0%
CTTCCTTCCTTAACACCAAC+TGG - Chr2:16873142-16873161 None:intergenic 45.0%
GAAGCGTAGTAGAGAGAAAG+AGG - Chr2:16871301-16871320 None:intergenic 45.0%
GAGAGTTTCATTCAGGGATC+AGG + Chr2:16873051-16873070 MsG0280007538.01.T01:CDS 45.0%
GCTAAGGAGAATGGAGAAAG+TGG + Chr2:16873409-16873428 MsG0280007538.01.T01:CDS 45.0%
GGAAGTTATGAAGGATGGTC+AGG + Chr2:16872156-16872175 MsG0280007538.01.T01:CDS 45.0%
GGAGAGTTAGTTGAGGTTGT+TGG + Chr2:16873079-16873098 MsG0280007538.01.T01:CDS 45.0%
GTTTCATTCAGGGATCAGGT+TGG + Chr2:16873055-16873074 MsG0280007538.01.T01:CDS 45.0%
TAGTTGGTCATTCTTCGGCT+TGG + Chr2:16871942-16871961 MsG0280007538.01.T01:CDS 45.0%
TCCTCGTAATCAGCAATGCT+TGG - Chr2:16873451-16873470 None:intergenic 45.0%
TCTAACCTTCCGTTCTTGTG+AGG - Chr2:16872295-16872314 None:intergenic 45.0%
TGCAAATGAGCGTTTGACCA+AGG + Chr2:16872727-16872746 MsG0280007538.01.T01:CDS 45.0%
TGTCAAAGTTGGATCAGCCT+GGG + Chr2:16873348-16873367 MsG0280007538.01.T01:CDS 45.0%
TGTCCCACTTCAAACTCAAC+AGG - Chr2:16872758-16872777 None:intergenic 45.0%
! ACTACCAAGCTCAGTGGATT+TGG - Chr2:16872969-16872988 None:intergenic 45.0%
! CATGGTTCTCAGAAAGGTGT+GGG + Chr2:16872016-16872035 MsG0280007538.01.T01:CDS 45.0%
! TCCAAGCATTGCTGATTACG+AGG + Chr2:16873447-16873466 MsG0280007538.01.T01:CDS 45.0%
! TGCATGTTTTCAACTCCCTG+TGG - Chr2:16873554-16873573 None:intergenic 45.0%
! TTCATTCAGGGATCAGGTTG+GGG + Chr2:16873057-16873076 MsG0280007538.01.T01:CDS 45.0%
!! CGTAAATCCCAGTTTTAGCG+GGG - Chr2:16873303-16873322 None:intergenic 45.0%
!! GAGCTTGGTAGTGCAACAAA+TGG + Chr2:16872977-16872996 MsG0280007538.01.T01:CDS 45.0%
!! GTGAGAGAATCGTACCGATT+AGG - Chr2:16871348-16871367 None:intergenic 45.0%
!! TGTTGATTCTGCCCCAACAA+CGG - Chr2:16872055-16872074 None:intergenic 45.0%
ACAACAGCCTCTTCATCCGA+TGG - Chr2:16872893-16872912 None:intergenic 50.0%
AGGATGGTCAGGTGCTAGAT+AGG + Chr2:16872167-16872186 MsG0280007538.01.T01:CDS 50.0%
AGGCAACTCAGATTGGTGGA+GGG + Chr2:16872074-16872093 MsG0280007538.01.T01:CDS 50.0%
CAACAGCCTCTTCATCCGAT+GGG - Chr2:16872892-16872911 None:intergenic 50.0%
CAACAGGCAACTCAGATTGG+TGG + Chr2:16872070-16872089 MsG0280007538.01.T01:CDS 50.0%
CTGTCAAAGTTGGATCAGCC+TGG + Chr2:16873347-16873366 MsG0280007538.01.T01:CDS 50.0%
GAAACAGAACCTGGTCCAGT+TGG + Chr2:16873124-16873143 MsG0280007538.01.T01:CDS 50.0%
GCATACTCACCTGTCAGTAG+TGG + Chr2:16873370-16873389 MsG0280007538.01.T01:CDS 50.0%
GTGTCCAAATCCACTGAGCT+TGG + Chr2:16872962-16872981 MsG0280007538.01.T01:CDS 50.0%
GTTGTTGGGGCAGAATCAAC+AGG + Chr2:16872054-16872073 MsG0280007538.01.T01:CDS 50.0%
TCCTTAACACCAACTGGACC+AGG - Chr2:16873136-16873155 None:intergenic 50.0%
TCTCCATTCTCCTTAGCTGC+TGG - Chr2:16873406-16873425 None:intergenic 50.0%
TGAATCTACCTCCCTAGCAG+AGG - Chr2:16873227-16873246 None:intergenic 50.0%
TGGTTCCTCACAAGAACGGA+AGG + Chr2:16872287-16872306 MsG0280007538.01.T01:CDS 50.0%
TGTTGCACTACCAAGCTCAG+TGG - Chr2:16872975-16872994 None:intergenic 50.0%
! TTCTCAGAAAGGTGTGGGAG+TGG + Chr2:16872021-16872040 MsG0280007538.01.T01:CDS 50.0%
!! ACCTGGTCCAGTTGGTGTTA+AGG + Chr2:16873132-16873151 MsG0280007538.01.T01:CDS 50.0%
!! GGTCCAGTTGGTGTTAAGGA+AGG + Chr2:16873136-16873155 MsG0280007538.01.T01:CDS 50.0%
!! TCATTCAGGGATCAGGTTGG+GGG + Chr2:16873058-16873077 MsG0280007538.01.T01:CDS 50.0%
!!! GCGACCGGGTGTTTTTTACT+TGG + Chr2:16872135-16872154 MsG0280007538.01.T01:CDS 50.0%
ACTGACAGGTGAGTATGCCC+AGG - Chr2:16873368-16873387 None:intergenic 55.0%
CAGGCAACTCAGATTGGTGG+AGG + Chr2:16872073-16872092 MsG0280007538.01.T01:CDS 55.0%
GAAAGGGCAGTCCTCTGCTA+GGG + Chr2:16873213-16873232 MsG0280007538.01.T01:CDS 55.0%
GATGTGGCAGCCACCAGATT+GGG + Chr2:16872102-16872121 MsG0280007538.01.T01:CDS 55.0%
GCGGTGGTTCCTCACAAGAA+CGG + Chr2:16872283-16872302 MsG0280007538.01.T01:CDS 55.0%
GCTCGTTCACCACTACTGAC+AGG - Chr2:16873382-16873401 None:intergenic 55.0%
GGTGGGAAATTGATCCCCGT+GGG - Chr2:16872860-16872879 None:intergenic 55.0%
GGTTGGGGGAGAGTTAGTTG+AGG + Chr2:16873072-16873091 MsG0280007538.01.T01:CDS 55.0%
TCCTGACCCATCGGATGAAG+AGG + Chr2:16872883-16872902 MsG0280007538.01.T01:CDS 55.0%
TCTTCATCCGATGGGTCAGG+AGG - Chr2:16872884-16872903 None:intergenic 55.0%
TGGTGGGAAATTGATCCCCG+TGG - Chr2:16872861-16872880 None:intergenic 55.0%
TGTCTCTCGCCAACATGCTG+CGG + Chr2:16872264-16872283 MsG0280007538.01.T01:CDS 55.0%
! TCAGAAAGGTGTGGGAGTGG+AGG + Chr2:16872024-16872043 MsG0280007538.01.T01:CDS 55.0%
CGATGTGGCAGCCACCAGAT+TGG + Chr2:16872101-16872120 MsG0280007538.01.T01:CDS 60.0%
GAGGAACCACCGCAGCATGT+TGG - Chr2:16872276-16872295 None:intergenic 60.0%
GAGGACTGCCCTTTCCCCTT+TGG - Chr2:16873208-16873227 None:intergenic 60.0%
GCCTCTTCATCCGATGGGTC+AGG - Chr2:16872887-16872906 None:intergenic 60.0%
GCGCCAGCAGCTAAGGAGAA+TGG + Chr2:16873400-16873419 MsG0280007538.01.T01:CDS 60.0%
GGAAAGGGCAGTCCTCTGCT+AGG + Chr2:16873212-16873231 MsG0280007538.01.T01:CDS 60.0%
GGCTCAATCGCCCAATCTGG+TGG - Chr2:16872115-16872134 None:intergenic 60.0%
GGGAAATTGATCCCCGTGGG+TGG - Chr2:16872857-16872876 None:intergenic 60.0%
TCATCCGATGGGTCAGGAGG+TGG - Chr2:16872881-16872900 None:intergenic 60.0%
TGAACGAGCGCCAGCAGCTA+AGG + Chr2:16873393-16873412 MsG0280007538.01.T01:CDS 60.0%
TTGATCCCCGTGGGTGGTGA+AGG - Chr2:16872851-16872870 None:intergenic 60.0%
TTGGTGGAGGGCAGTCGATG+TGG + Chr2:16872086-16872105 MsG0280007538.01.T01:CDS 60.0%
! GAAAGGTGTGGGAGTGGAGG+CGG + Chr2:16872027-16872046 MsG0280007538.01.T01:CDS 60.0%
AGGGCAGTCCTCTGCTAGGG+AGG + Chr2:16873216-16873235 MsG0280007538.01.T01:CDS 65.0%
AGTGGAGGCGGCTCCGTTGT+TGG + Chr2:16872039-16872058 MsG0280007538.01.T01:CDS 65.0%
CCCACCACCTCCTGACCCAT+CGG + Chr2:16872874-16872893 MsG0280007538.01.T01:CDS 65.0%
CCCTCGTCCTTCACCACCCA+CGG + Chr2:16872841-16872860 MsG0280007538.01.T01:CDS 65.0%
CCGATGGGTCAGGAGGTGGT+GGG - Chr2:16872877-16872896 None:intergenic 65.0%
CCGTGGGTGGTGAAGGACGA+GGG - Chr2:16872844-16872863 None:intergenic 65.0%
CCTCGTCCTTCACCACCCAC+GGG + Chr2:16872842-16872861 MsG0280007538.01.T01:CDS 65.0%
CGCGGCTCAATCGCCCAATC+TGG - Chr2:16872118-16872137 None:intergenic 65.0%
CTCGTCCTTCACCACCCACG+GGG + Chr2:16872843-16872862 MsG0280007538.01.T01:CDS 65.0%
CTCTCGCCAACATGCTGCGG+TGG + Chr2:16872267-16872286 MsG0280007538.01.T01:CDS 65.0%
GTGGAGGCGGCTCCGTTGTT+GGG + Chr2:16872040-16872059 MsG0280007538.01.T01:CDS 65.0%
TCCGATGGGTCAGGAGGTGG+TGG - Chr2:16872878-16872897 None:intergenic 65.0%
TGGAGGCGGCTCCGTTGTTG+GGG + Chr2:16872041-16872060 MsG0280007538.01.T01:CDS 65.0%
TTGGGCGATTGAGCCGCGAC+CGG + Chr2:16872120-16872139 MsG0280007538.01.T01:CDS 65.0%
CCCGTGGGTGGTGAAGGACG+AGG - Chr2:16872845-16872864 None:intergenic 70.0%
TGGGCGATTGAGCCGCGACC+GGG + Chr2:16872121-16872140 MsG0280007538.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 16871275 16873713 16871275 ID=MsG0280007538.01;Name=MsG0280007538.01
Chr2 mRNA 16871275 16873713 16871275 ID=MsG0280007538.01.T01;Parent=MsG0280007538.01;Name=MsG0280007538.01.T01;_AED=0.41;_eAED=0.41;_QI=200|1|1|1|0.5|0.33|3|221|428
Chr2 exon 16871275 16871352 16871275 ID=MsG0280007538.01.T01:exon:3600;Parent=MsG0280007538.01.T01
Chr2 exon 16871708 16872308 16871708 ID=MsG0280007538.01.T01:exon:3601;Parent=MsG0280007538.01.T01
Chr2 exon 16872685 16873713 16872685 ID=MsG0280007538.01.T01:exon:3602;Parent=MsG0280007538.01.T01
Chr2 five_prime_UTR 16871275 16871352 16871275 ID=MsG0280007538.01.T01:five_prime_utr;Parent=MsG0280007538.01.T01
Chr2 five_prime_UTR 16871708 16871829 16871708 ID=MsG0280007538.01.T01:five_prime_utr;Parent=MsG0280007538.01.T01
Chr2 CDS 16871830 16872308 16871830 ID=MsG0280007538.01.T01:cds;Parent=MsG0280007538.01.T01
Chr2 CDS 16872685 16873492 16872685 ID=MsG0280007538.01.T01:cds;Parent=MsG0280007538.01.T01
Chr2 three_prime_UTR 16873493 16873713 16873493 ID=MsG0280007538.01.T01:three_prime_utr;Parent=MsG0280007538.01.T01
Gene Sequence

>MsG0280007538.01.T01

ATGTATGGAAGAACATCTGGTCTTGATAGGTTTAAGAAAGCCGAGAGTTTGGAACCGTTCTCTGTTAATGGTAATTCGTCTTCGAGAAATGTTGCTCAGTCTTCTAGTAAGGTAGTTGGTCATTCTTCGGCTTGGCTGCCTCCACAGTCGAAGAATTCTGTACATCAATCACAGAATCAACATCAACATGGTTCTCAGAAAGGTGTGGGAGTGGAGGCGGCTCCGTTGTTGGGGCAGAATCAACAGGCAACTCAGATTGGTGGAGGGCAGTCGATGTGGCAGCCACCAGATTGGGCGATTGAGCCGCGACCGGGTGTTTTTTACTTGGAAGTTATGAAGGATGGTCAGGTGCTAGATAGGATTAATTTGGATAGACGAAGAAATATTTTTGGGAGGCAAATCCAGACTTGTGATTTTGTGCTTGATCATCAATCTGTCTCTCGCCAACATGCTGCGGTGGTTCCTCACAAGAACGGAAGTGTATATGTAATTGATTTGGGATCTGCACATGGTACCTTTGTTGCAAATGAGCGTTTGACCAAGGATTCACCTGTTGAGTTTGAAGTGGGACAATCATTGCGGTTTGCTGCATCCACGAGATTATACATATTAAGAAAGAATGATGCAGCTCTTTTCCCTCGTCCTTCACCACCCACGGGGATCAATTTCCCACCACCTCCTGACCCATCGGATGAAGAGGCTGTTGTTACTTATAATACGCTGCTGAATCGTTATGGCATCAACAAGTCTGATCTCGTGTCCAAATCCACTGAGCTTGGTAGTGCAACAAATGGCAAAAACAAGGATTGCCAATCAGAAAGAGCTGCTAAGAGAATTAAGAAGATGAGAGTTTCATTCAGGGATCAGGTTGGGGGAGAGTTAGTTGAGGTTGTTGGAATTTCAGATGGTGTAGATGTAGAAACAGAACCTGGTCCAGTTGGTGTTAAGGAAGGAAGTCTAGTTGGTAAATATGAATCTCTTGTACAAACTACTGTAATTCCAAAGGGGAAAGGGCAGTCCTCTGCTAGGGAGGTAGATTCATCCCAAAAAGGTGTGACTGATAAACTACAAGAAGTCCTAAACAAGATCAAAAACCCCGCTAAAACTGGGATTTACGATGACCTATATGGAGAATCATTATCTGTCAAAGTTGGATCAGCCTGGGCATACTCACCTGTCAGTAGTGGTGAACGAGCGCCAGCAGCTAAGGAGAATGGAGAAAGTGGAAAATCAGACAGTAATCCAAGCATTGCTGATTACGAGGATGATGATGATTTGTTTGGGTGA
Protein sequence

>MsG0280007538.01.T01

MYGRTSGLDRFKKAESLEPFSVNGNSSSRNVAQSSSKVVGHSSAWLPPQSKNSVHQSQNQHQHGSQKGVGVEAAPLLGQNQQATQIGGGQSMWQPPDWAIEPRPGVFYLEVMKDGQVLDRINLDRRRNIFGRQIQTCDFVLDHQSVSRQHAAVVPHKNGSVYVIDLGSAHGTFVANERLTKDSPVEFEVGQSLRFAASTRLYILRKNDAALFPRPSPPTGINFPPPPDPSDEEAVVTYNTLLNRYGINKSDLVSKSTELGSATNGKNKDCQSERAAKRIKKMRVSFRDQVGGELVEVVGISDGVDVETEPGPVGVKEGSLVGKYESLVQTTVIPKGKGQSSAREVDSSQKGVTDKLQEVLNKIKNPAKTGIYDDLYGESLSVKVGSAWAYSPVSSGERAPAAKENGESGKSDSNPSIADYEDDDDLFG*