Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007652.01.T01 | XP_013462997.2 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.21E-91 | 285 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007652.01.T01 | P54770 | 74.074 | 135 | 35 | 0 | 2 | 136 | 20 | 154 | 3.88E-73 | 230 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007652.01.T01 | A0A396J8B4 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 5.80e-92 | 285 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007652.01 | MsG0280007653.01 | 0.803615 | 3.203598e-49 | 1.617226e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007652.01.T01 | MTR_2g029430 | 98.529 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 5.01e-95 | 283 |
| MsG0280007652.01.T01 | MTR_2g029450 | 98.529 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 5.01e-95 | 283 |
| MsG0280007652.01.T01 | MTR_7g058700 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 1.25e-90 | 281 |
| MsG0280007652.01.T01 | MTR_8g028060 | 75.735 | 136 | 33 | 0 | 1 | 136 | 17 | 152 | 3.19e-73 | 227 |
| MsG0280007652.01.T01 | MTR_7g098730 | 61.314 | 137 | 53 | 0 | 1 | 137 | 24 | 160 | 1.75e-61 | 196 |
| MsG0280007652.01.T01 | MTR_7g098700 | 61.314 | 137 | 53 | 0 | 1 | 137 | 50 | 186 | 3.55e-61 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007652.01.T01 | AT2G20340 | 55.474 | 137 | 61 | 0 | 1 | 137 | 9 | 145 | 1.28e-59 | 191 |
| MsG0280007652.01.T01 | AT2G20340 | 55.474 | 137 | 61 | 0 | 1 | 137 | 9 | 145 | 3.11e-59 | 190 |
| MsG0280007652.01.T01 | AT4G28680 | 58.156 | 141 | 55 | 1 | 1 | 137 | 48 | 188 | 2.14e-58 | 185 |
| MsG0280007652.01.T01 | AT4G28680 | 58.156 | 141 | 55 | 1 | 1 | 137 | 48 | 188 | 3.73e-58 | 186 |
| MsG0280007652.01.T01 | AT4G28680 | 58.156 | 141 | 55 | 1 | 1 | 137 | 48 | 188 | 1.61e-57 | 187 |
| MsG0280007652.01.T01 | AT4G28680 | 58.156 | 141 | 55 | 1 | 1 | 137 | 57 | 197 | 2.10e-57 | 187 |
| MsG0280007652.01.T01 | AT4G28680 | 58.156 | 141 | 55 | 1 | 1 | 137 | 57 | 197 | 2.13e-57 | 187 |
| MsG0280007652.01.T01 | AT4G28680 | 58.156 | 141 | 55 | 1 | 1 | 137 | 57 | 197 | 2.22e-57 | 187 |
| MsG0280007652.01.T01 | AT4G28680 | 58.156 | 141 | 55 | 1 | 1 | 137 | 48 | 188 | 3.72e-57 | 186 |
Find 22 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTAGCACAGCAGGGTTCTT+AGG | 0.266911 | 2:+18593375 | MsG0280007652.01.T01:CDS |
| GGAAAATAAGCAAAGTAATT+AGG | 0.271671 | 2:-18593345 | None:intergenic |
| TAGAAAATGTTGTTATAGAT+TGG | 0.319360 | 2:+18593466 | MsG0280007652.01.T01:CDS |
| CCTGCTGTGCTACCACTTGA+TGG | 0.350739 | 2:-18593366 | None:intergenic |
| TTGAATTCTGTAGTATCTAG+TGG | 0.355450 | 2:-18593123 | None:intergenic |
| TGAATGTTGTTGGATTTAAC+TGG | 0.365518 | 2:+18593418 | MsG0280007652.01.T01:CDS |
| CCATCAAGTGGTAGCACAGC+AGG | 0.386882 | 2:+18593366 | MsG0280007652.01.T01:CDS |
| AGGATGGTTTCAATAGGTTC+AGG | 0.403995 | 2:-18593273 | None:intergenic |
| TCTTCAAGGATGGTTTCAAT+AGG | 0.409255 | 2:-18593279 | None:intergenic |
| AACACTGGATTGAATGTTGT+TGG | 0.419304 | 2:+18593408 | MsG0280007652.01.T01:CDS |
| CTTTGCCAATGTGTTATACC+TGG | 0.443144 | 2:-18593321 | None:intergenic |
| AGTTTGGTTCTACTTGACTT+AGG | 0.478980 | 2:-18593212 | None:intergenic |
| TGAATTCTGTAGTATCTAGT+GGG | 0.505218 | 2:-18593122 | None:intergenic |
| GGATTTGAAGGTGCAAAAGA+AGG | 0.506622 | 2:-18593252 | None:intergenic |
| TTAGGAGAAATGTTAAACAC+TGG | 0.513794 | 2:+18593393 | MsG0280007652.01.T01:CDS |
| TCAATAGGTTCAGGATTTGA+AGG | 0.516728 | 2:-18593264 | None:intergenic |
| GGTTCTACTTGACTTAGGAC+AGG | 0.523508 | 2:-18593207 | None:intergenic |
| AATGTTGTTATAGATTGGCT+TGG | 0.556855 | 2:+18593471 | MsG0280007652.01.T01:CDS |
| TTTGCCAATGTGTTATACCT+GGG | 0.567911 | 2:-18593320 | None:intergenic |
| CATCAAGTGGTAGCACAGCA+GGG | 0.607312 | 2:+18593367 | MsG0280007652.01.T01:CDS |
| TAATCCCAGGTATAACACAT+TGG | 0.657713 | 2:+18593316 | MsG0280007652.01.T01:CDS |
| ACTACAGAATTCAAGAAACA+AGG | 0.665050 | 2:+18593132 | MsG0280007652.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAAATGTTTTCATAATAAT+CGG | - | Chr2:18593181-18593200 | None:intergenic | 15.0% |
| !! | TAGAAAATGTTGTTATAGAT+TGG | + | Chr2:18593466-18593485 | MsG0280007652.01.T01:CDS | 20.0% |
| ! | GGAAAATAAGCAAAGTAATT+AGG | - | Chr2:18593348-18593367 | None:intergenic | 25.0% |
| !!! | TGTGTTTTTGATAATCTTCA+AGG | - | Chr2:18593296-18593315 | None:intergenic | 25.0% |
| !!! | TTTTTGATAATCTTCAAGGA+TGG | - | Chr2:18593292-18593311 | None:intergenic | 25.0% |
| ACTACAGAATTCAAGAAACA+AGG | + | Chr2:18593132-18593151 | MsG0280007652.01.T01:CDS | 30.0% | |
| TATCAAAAACACATAATCCC+AGG | + | Chr2:18593303-18593322 | MsG0280007652.01.T01:CDS | 30.0% | |
| TGAATGTTGTTGGATTTAAC+TGG | + | Chr2:18593418-18593437 | MsG0280007652.01.T01:CDS | 30.0% | |
| TGAATTCTGTAGTATCTAGT+GGG | - | Chr2:18593125-18593144 | None:intergenic | 30.0% | |
| TTAGGAGAAATGTTAAACAC+TGG | + | Chr2:18593393-18593412 | MsG0280007652.01.T01:CDS | 30.0% | |
| TTGAATTCTGTAGTATCTAG+TGG | - | Chr2:18593126-18593145 | None:intergenic | 30.0% | |
| ! | AATGTTGTTATAGATTGGCT+TGG | + | Chr2:18593471-18593490 | MsG0280007652.01.T01:CDS | 30.0% |
| ! | TGTTTTCATAATAATCGGCT+AGG | - | Chr2:18593176-18593195 | None:intergenic | 30.0% |
| !! | TTTGCTTATTTTCCATCAAG+TGG | + | Chr2:18593354-18593373 | MsG0280007652.01.T01:CDS | 30.0% |
| !!! | AACAGTTTTTTGAGGTAGTT+TGG | - | Chr2:18593231-18593250 | None:intergenic | 30.0% |
| !!! | AAGAAGGTAACAGTTTTTTG+AGG | - | Chr2:18593239-18593258 | None:intergenic | 30.0% |
| AACACTGGATTGAATGTTGT+TGG | + | Chr2:18593408-18593427 | MsG0280007652.01.T01:CDS | 35.0% | |
| TAATCCCAGGTATAACACAT+TGG | + | Chr2:18593316-18593335 | MsG0280007652.01.T01:CDS | 35.0% | |
| TCAATAGGTTCAGGATTTGA+AGG | - | Chr2:18593267-18593286 | None:intergenic | 35.0% | |
| TCTTCAAGGATGGTTTCAAT+AGG | - | Chr2:18593282-18593301 | None:intergenic | 35.0% | |
| TTTGCCAATGTGTTATACCT+GGG | - | Chr2:18593323-18593342 | None:intergenic | 35.0% | |
| !! | AGTTTGGTTCTACTTGACTT+AGG | - | Chr2:18593215-18593234 | None:intergenic | 35.0% |
| AGGATGGTTTCAATAGGTTC+AGG | - | Chr2:18593276-18593295 | None:intergenic | 40.0% | |
| CTTTGCCAATGTGTTATACC+TGG | - | Chr2:18593324-18593343 | None:intergenic | 40.0% | |
| ! | GGATTTGAAGGTGCAAAAGA+AGG | - | Chr2:18593255-18593274 | None:intergenic | 40.0% |
| !!! | TTTTCTAGTTCTGTTGCAGC+TGG | - | Chr2:18593453-18593472 | None:intergenic | 40.0% |
| GGTTCTACTTGACTTAGGAC+AGG | - | Chr2:18593210-18593229 | None:intergenic | 45.0% | |
| CATCAAGTGGTAGCACAGCA+GGG | + | Chr2:18593367-18593386 | MsG0280007652.01.T01:CDS | 50.0% | |
| CCATCAAGTGGTAGCACAGC+AGG | + | Chr2:18593366-18593385 | MsG0280007652.01.T01:CDS | 55.0% | |
| CCTGCTGTGCTACCACTTGA+TGG | - | Chr2:18593369-18593388 | None:intergenic | 55.0% | |
| GGTAGCACAGCAGGGTTCTT+AGG | + | Chr2:18593375-18593394 | MsG0280007652.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 18593117 | 18593530 | 18593117 | ID=MsG0280007652.01;Name=MsG0280007652.01 |
| Chr2 | mRNA | 18593117 | 18593530 | 18593117 | ID=MsG0280007652.01.T01;Parent=MsG0280007652.01;Name=MsG0280007652.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|137 |
| Chr2 | exon | 18593117 | 18593530 | 18593117 | ID=MsG0280007652.01.T01:exon:4259;Parent=MsG0280007652.01.T01 |
| Chr2 | CDS | 18593117 | 18593530 | 18593117 | ID=MsG0280007652.01.T01:cds;Parent=MsG0280007652.01.T01 |
| Gene Sequence |
| Protein sequence |
