AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008056.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008056.01.T01 MTR_8g100045 93.333 495 27 1 1 489 1 495 0.0 931
MsG0280008056.01.T01 MTR_6g011360 93.131 495 28 1 1 489 1 495 0.0 929
MsG0280008056.01.T01 MTR_3g085010 66.667 117 31 2 1 109 1 117 6.70e-38 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008056.01.T01 AT1G74260 70.782 486 130 4 12 489 12 493 0.0 705

Find 125 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTCAGTTATTGCCTTTA+AGG 0.016138 2:+24883482 MsG0280008056.01.T01:CDS
TGCACTTGAGTTATCCTTAA+AGG 0.139846 2:-24883496 None:intergenic
CTTAACTTATCCATCAAATT+TGG 0.191400 2:+24883797 MsG0280008056.01.T01:CDS
TCTGACTACGGGAACAAATT+TGG 0.198177 2:+24883861 MsG0280008056.01.T01:CDS
AGGATAACTCAAGTGCAATT+AGG 0.221934 2:+24883502 MsG0280008056.01.T01:CDS
ACATTTGAGCCTGAGAATTT+AGG 0.230585 2:+24882880 MsG0280008056.01.T01:CDS
ACCTAAAATTCAATTGCTTT+GGG 0.242562 2:+24882537 MsG0280008056.01.T01:CDS
TTTGGGGTAACCTTTGTAAT+AGG 0.247021 2:+24882554 MsG0280008056.01.T01:CDS
ATGGCAGCTGCTGGAGAAAT+TGG 0.249872 2:+24882354 MsG0280008056.01.T01:CDS
GGATAACTCAAGTGCAATTA+GGG 0.261997 2:+24883503 MsG0280008056.01.T01:CDS
AATTGTGAAAAGCCCTTTAC+AGG 0.264854 2:+24883449 MsG0280008056.01.T01:CDS
AACCTAAAATTCAATTGCTT+TGG 0.265434 2:+24882536 MsG0280008056.01.T01:CDS
TTGGGGTAACCTTTGTAATA+GGG 0.279065 2:+24882555 MsG0280008056.01.T01:CDS
GCTCGGCAGCTGCACTTTCT+TGG 0.285137 2:-24882726 None:intergenic
TGCATTTGATGACCAGGATT+TGG 0.305481 2:+24883269 MsG0280008056.01.T01:CDS
GGGATACACATGCAACAATA+AGG 0.310206 2:+24883688 MsG0280008056.01.T01:CDS
CAGCTGCAGGAGGTCGCATT+AGG 0.311838 2:+24883667 MsG0280008056.01.T01:CDS
AAGGCAATAACTGAGTTGTT+TGG 0.337397 2:-24883477 None:intergenic
CGAACGAACCGGAAGGAATT+CGG 0.344460 2:-24882382 None:intergenic
AGTATCTTCTCTGAAAAGTT+TGG 0.346625 2:-24883301 None:intergenic
CCTTTCAAGTGGAAAGCTTT+CGG 0.354181 2:+24882834 MsG0280008056.01.T01:CDS
GTTTCAGTAGAACAACATTC+TGG 0.360340 2:+24882649 MsG0280008056.01.T01:CDS
TTCAAAGGATGTTAGCTTCT+GGG 0.362609 2:-24883157 None:intergenic
TTTCAAAGGATGTTAGCTTC+TGG 0.362642 2:-24883158 None:intergenic
GAAAGCTTTCGGTTCTTAGA+TGG 0.368377 2:+24882845 MsG0280008056.01.T01:CDS
CTTTGAAACCAGTGTAGTTC+CGG 0.387550 2:+24883173 MsG0280008056.01.T01:CDS
CTTGTTGAACCGGGCTGAAC+CGG 0.387950 2:-24883549 None:intergenic
TCGCGTTCTGTCAAGTTTAA+CGG 0.388778 2:-24883573 None:intergenic
TGTTTGGATTTGCCTGTAAA+GGG 0.394430 2:-24883461 None:intergenic
TGGCAGCTGCTGGAGAAATT+GGG 0.399154 2:+24882355 MsG0280008056.01.T01:CDS
CTACAGCTGGTTATTGTGTT+GGG 0.404206 2:+24883730 MsG0280008056.01.T01:CDS
CAAATTTGATGGATAAGTTA+AGG 0.404998 2:-24883796 None:intergenic
TGCATCAGAGTCTTATTCAT+AGG 0.405859 2:-24883426 None:intergenic
TCTACTGAAACCACAGCTCT+TGG 0.414040 2:-24882637 None:intergenic
CCAAGGCTTCTGTAGAACTT+TGG 0.414213 2:+24883896 MsG0280008056.01.T01:CDS
TGGGGTATCCGAATTCCTTC+CGG 0.414805 2:+24882374 MsG0280008056.01.T01:CDS
AGTAGAACAACATTCTGGTT+TGG 0.426906 2:+24882654 MsG0280008056.01.T01:CDS
CCGGTCGTAATGGCTTTACT+TGG 0.429494 2:-24883530 None:intergenic
CAATGCTTTAATGTTGGCCT+TGG 0.431435 2:+24882805 MsG0280008056.01.T01:CDS
CTCTCAGTTCCTAAATTCTC+AGG 0.433195 2:-24882889 None:intergenic
TCCTGCAGCTGTCTCTGCAC+CGG 0.433344 2:-24883655 None:intergenic
GCTGTCTCTGCACCGGGATA+AGG 0.433447 2:-24883648 None:intergenic
AATTAATGAATTTGCAGCTA+TGG 0.442315 2:+24883104 MsG0280008056.01.T01:CDS
GGAAGAGATTAATCTGAAGA+TGG 0.442649 2:+24883242 MsG0280008056.01.T01:CDS
GGATACACATGCAACAATAA+GGG 0.446112 2:+24883689 MsG0280008056.01.T01:CDS
CCAAAGTTCTACAGAAGCCT+TGG 0.449383 2:-24883896 None:intergenic
TTGTTTGGATTTGCCTGTAA+AGG 0.450883 2:-24883462 None:intergenic
AGGTATTTGTTGTATACAAA+TGG 0.451120 2:+24883063 MsG0280008056.01.T01:CDS
TGAATCGTTTGGAGCGTTCA+AGG 0.453405 2:+24883040 MsG0280008056.01.T01:CDS
GTTTAACGGACTTGTTGAAC+CGG 0.455950 2:-24883559 None:intergenic
TTCCTCCCCTTCTCCATGAC+AGG 0.459185 2:-24883213 None:intergenic
GAAGAGATTAATCTGAAGAT+GGG 0.459755 2:+24883243 MsG0280008056.01.T01:CDS
GCTGCACTTTCTTGGATAAA+CGG 0.460430 2:-24882718 None:intergenic
TTTGACAGAAGTGAATCGTT+TGG 0.466480 2:+24883029 MsG0280008056.01.T01:CDS
GCTACAGCTGGTTATTGTGT+TGG 0.467847 2:+24883729 MsG0280008056.01.T01:CDS
GTTGGGAATTTCAACACAGC+AGG 0.468946 2:+24883747 MsG0280008056.01.T01:CDS
AGCTGCAGGAGGTCGCATTA+GGG 0.474924 2:+24883668 MsG0280008056.01.T01:CDS
TTGCAGCTATGGTGCATGAT+AGG 0.475154 2:+24883115 MsG0280008056.01.T01:CDS
AGTCCAACAGTGAGCATAGC+AGG 0.476721 2:+24883364 MsG0280008056.01.T01:CDS
GGAGAAGGGGAGGAAGGCAT+TGG 0.478874 2:+24883221 MsG0280008056.01.T01:CDS
AGTAATGGTGCTTCTGACTA+CGG 0.482762 2:+24883849 MsG0280008056.01.T01:CDS
CCGGGCTGAACCGGTCGTAA+TGG 0.483576 2:-24883540 None:intergenic
TCAAACAATTCCACATTCGT+TGG 0.484049 2:-24883333 None:intergenic
TGGTGTAGTATTCCAAATCC+TGG 0.491089 2:-24883281 None:intergenic
ATCGAGTATATGGCAGCTGC+TGG 0.492858 2:+24882345 None:intergenic
AAAGGCGGTGCCAAATTTGA+TGG 0.496625 2:-24883807 None:intergenic
AAACACGACCCCTATTACAA+AGG 0.500701 2:-24882564 None:intergenic
ACTGAACAATGCTTTAATGT+TGG 0.504572 2:+24882799 MsG0280008056.01.T01:CDS
CGGAACTACACTGGTTTCAA+AGG 0.507963 2:-24883172 None:intergenic
TGCTGTGGTAAATGATAACC+TGG 0.511282 2:-24882968 None:intergenic
CCTGCAGCTGTCTCTGCACC+GGG 0.513248 2:-24883654 None:intergenic
TATATACCTGTCATGGAGAA+GGG 0.516133 2:+24883207 MsG0280008056.01.T01:CDS
CCGAAAGCTTTCCACTTGAA+AGG 0.517820 2:-24882834 None:intergenic
CCCGGTGCAGAGACAGCTGC+AGG 0.517849 2:+24883654 MsG0280008056.01.T01:CDS
GAGAAAGTTATAGTTGAGGT+TGG 0.518926 2:+24882943 MsG0280008056.01.T01:CDS
TTATATACCTGTCATGGAGA+AGG 0.519219 2:+24883206 MsG0280008056.01.T01:CDS
CCTAAAATTCAATTGCTTTG+GGG 0.520206 2:+24882538 MsG0280008056.01.T01:CDS
TTTGTGCAAGCAGCTACAGC+TGG 0.521421 2:+24883717 MsG0280008056.01.T01:CDS
ATTCTTATTGATGCAAGTAA+TGG 0.523805 2:+24883834 MsG0280008056.01.T01:CDS
CAGCAGGGCTCTATGCTCCA+TGG 0.525754 2:+24883763 MsG0280008056.01.T01:CDS
GAAGATCTCTAATCAGATAG+TGG 0.526952 2:+24882768 MsG0280008056.01.T01:CDS
CTTCTGTCAAACCACATGCT+TGG 0.528607 2:-24883017 None:intergenic
GTAATGGTGCTTCTGACTAC+GGG 0.530953 2:+24883850 MsG0280008056.01.T01:CDS
GGTATATAATAAAATTCCTC+CGG 0.531568 2:-24883192 None:intergenic
TTTGGAGAAAGTTATAGTTG+AGG 0.534488 2:+24882939 MsG0280008056.01.T01:CDS
TTGAGCCTGCTTTAAAAGCT+CGG 0.540210 2:-24882743 None:intergenic
CTACAGAAGCCTTGGATCAA+CGG 0.544030 2:-24883888 None:intergenic
TTTGGAGAGCCGTTGATCCA+AGG 0.548214 2:+24883879 MsG0280008056.01.T01:CDS
CCAAGTAAAGCCATTACGAC+CGG 0.550856 2:+24883530 MsG0280008056.01.T01:CDS
CCATTACGACCGGTTCAGCC+CGG 0.555069 2:+24883540 MsG0280008056.01.T01:CDS
TGGGGTAACCTTTGTAATAG+GGG 0.563812 2:+24882556 MsG0280008056.01.T01:CDS
ATGCCTGCTATGCTCACTGT+TGG 0.565027 2:-24883367 None:intergenic
AGCAGGGCTCTATGCTCCAT+GGG 0.567428 2:+24883764 MsG0280008056.01.T01:CDS
AGATGAATAACTTGAGCAGA+AGG 0.570230 2:-24882685 None:intergenic
ACAGTGAGCATAGCAGGCAT+TGG 0.573894 2:+24883370 MsG0280008056.01.T01:CDS
AAATTCCTCCGGAACTACAC+TGG 0.576201 2:-24883181 None:intergenic
TGTCATGGAGAAGGGGAGGA+AGG 0.576678 2:+24883215 MsG0280008056.01.T01:CDS
TTGGCAAATTGACACAGCAT+TGG 0.576958 2:-24882998 None:intergenic
CTTGGTTCACACCTTTCAAG+TGG 0.580210 2:+24882823 MsG0280008056.01.T01:CDS
GGATAAGGTACCACTGCACA+TGG 0.580484 2:-24883633 None:intergenic
TTATAGTTGAGGTTGGCCCC+AGG 0.582586 2:+24882950 MsG0280008056.01.T01:CDS
TGTGCAGTGGTACCTTATCC+CGG 0.582967 2:+24883636 MsG0280008056.01.T01:CDS
TAAGCGTAATCCAACGAATG+TGG 0.589538 2:+24883323 MsG0280008056.01.T01:CDS
TTTAACGGACTTGTTGAACC+GGG 0.591519 2:-24883558 None:intergenic
GGCAGCTGCTGGAGAAATTG+GGG 0.591848 2:+24882356 MsG0280008056.01.T01:CDS
CACTTGAAAGGTGTGAACCA+AGG 0.595783 2:-24882822 None:intergenic
GATACACATGCAACAATAAG+GGG 0.601493 2:+24883690 MsG0280008056.01.T01:CDS
GTGTCAATTTGCCAAGCATG+TGG 0.601599 2:+24883006 MsG0280008056.01.T01:CDS
TTTGATGGATAAGTTAAGGA+CGG 0.606660 2:-24883792 None:intergenic
TACCTGTCATGGAGAAGGGG+AGG 0.621087 2:+24883211 MsG0280008056.01.T01:CDS
TTGGGAATTTCAACACAGCA+GGG 0.628083 2:+24883748 MsG0280008056.01.T01:CDS
GTTAAGGACGGATCTTCCCA+TGG 0.631326 2:-24883780 None:intergenic
ATATACCTGTCATGGAGAAG+GGG 0.633344 2:+24883208 MsG0280008056.01.T01:CDS
TGAAACCAGTGTAGTTCCGG+AGG 0.635030 2:+24883176 MsG0280008056.01.T01:CDS
GGTTATCATTTACCACAGCA+TGG 0.638162 2:+24882971 MsG0280008056.01.T01:CDS
TTTCTTGGATAAACGGTACA+CGG 0.638440 2:-24882711 None:intergenic
TCAAGAAAATCCAAGAGCTG+TGG 0.649955 2:+24882627 MsG0280008056.01.T01:CDS
TCAATAAGAATCTGCAAAGG+CGG 0.653825 2:-24883822 None:intergenic
GGTGCAGAGACAGCTGCAGG+AGG 0.657159 2:+24883657 MsG0280008056.01.T01:CDS
GATAACTCAAGTGCAATTAG+GGG 0.659007 2:+24883504 MsG0280008056.01.T01:CDS
GCATCAATAAGAATCTGCAA+AGG 0.685843 2:-24883825 None:intergenic
AGCATTGGCAGACCATGCTG+TGG 0.710447 2:-24882983 None:intergenic
ATCGTTTGGAGCGTTCAAGG+AGG 0.737771 2:+24883043 MsG0280008056.01.T01:CDS
GCTGTGGTAAATGATAACCT+GGG 0.749529 2:-24882967 None:intergenic
CTGTGGTAAATGATAACCTG+GGG 0.754824 2:-24882966 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAAATTGTGTTGAT+GGG + Chr2:24882450-24882469 MsG0280008056.01.T01:intron 15.0%
!! TTTAAGCTAATTGAAACAAA+GGG - Chr2:24882421-24882440 None:intergenic 20.0%
!!! ATTTTATTATATACCTGTCA+TGG + Chr2:24883200-24883219 MsG0280008056.01.T01:CDS 20.0%
!!! GTTTTTTTAAATTGTGTTGA+TGG + Chr2:24882449-24882468 MsG0280008056.01.T01:intron 20.0%
!!! TTTTAAGCTAATTGAAACAA+AGG - Chr2:24882422-24882441 None:intergenic 20.0%
!!! TTTTTTAAATTGTGTTGATG+GGG + Chr2:24882451-24882470 MsG0280008056.01.T01:intron 20.0%
! AACCTAAAATTCAATTGCTT+TGG + Chr2:24882536-24882555 MsG0280008056.01.T01:CDS 25.0%
! AATTAATGAATTTGCAGCTA+TGG + Chr2:24883104-24883123 MsG0280008056.01.T01:CDS 25.0%
! ACCTAAAATTCAATTGCTTT+GGG + Chr2:24882537-24882556 MsG0280008056.01.T01:CDS 25.0%
! ATTCTTATTGATGCAAGTAA+TGG + Chr2:24883834-24883853 MsG0280008056.01.T01:CDS 25.0%
! CTTAACTTATCCATCAAATT+TGG + Chr2:24883797-24883816 MsG0280008056.01.T01:CDS 25.0%
! GGTATATAATAAAATTCCTC+CGG - Chr2:24883195-24883214 None:intergenic 25.0%
!! CAAATTTGATGGATAAGTTA+AGG - Chr2:24883799-24883818 None:intergenic 25.0%
!!! AATTGAATTTTAGGTTTCTG+AGG - Chr2:24882532-24882551 None:intergenic 25.0%
!!! AGGTATTTGTTGTATACAAA+TGG + Chr2:24883063-24883082 MsG0280008056.01.T01:CDS 25.0%
ACTGAACAATGCTTTAATGT+TGG + Chr2:24882799-24882818 MsG0280008056.01.T01:CDS 30.0%
CCTAAAATTCAATTGCTTTG+GGG + Chr2:24882538-24882557 MsG0280008056.01.T01:CDS 30.0%
GAAGAGATTAATCTGAAGAT+GGG + Chr2:24883243-24883262 MsG0280008056.01.T01:CDS 30.0%
TAAAATAAAGTCGAACGAAC+CGG - Chr2:24882396-24882415 None:intergenic 30.0%
TCCGTCAATAAAAATCTTTC+CGG - Chr2:24883403-24883422 None:intergenic 30.0%
TTTGGAGAAAGTTATAGTTG+AGG + Chr2:24882939-24882958 MsG0280008056.01.T01:CDS 30.0%
! ACCGGAAAGATTTTTATTGA+CGG + Chr2:24883399-24883418 MsG0280008056.01.T01:CDS 30.0%
! AGTATCTTCTCTGAAAAGTT+TGG - Chr2:24883304-24883323 None:intergenic 30.0%
! TTTGATGGATAAGTTAAGGA+CGG - Chr2:24883795-24883814 None:intergenic 30.0%
!!! GGTTTATGATTTTGGTTACA+GGG + Chr2:24882472-24882491 MsG0280008056.01.T01:intron 30.0%
AAGGCAATAACTGAGTTGTT+TGG - Chr2:24883480-24883499 None:intergenic 35.0%
AATTGTGAAAAGCCCTTTAC+AGG + Chr2:24883449-24883468 MsG0280008056.01.T01:CDS 35.0%
ACATTTGAGCCTGAGAATTT+AGG + Chr2:24882880-24882899 MsG0280008056.01.T01:CDS 35.0%
AGAGCTTTCTTGAGAAAAAG+AGG + Chr2:24882908-24882927 MsG0280008056.01.T01:CDS 35.0%
AGATGAATAACTTGAGCAGA+AGG - Chr2:24882688-24882707 None:intergenic 35.0%
AGGATAACTCAAGTGCAATT+AGG + Chr2:24883502-24883521 MsG0280008056.01.T01:CDS 35.0%
CAACTCAGTTATTGCCTTTA+AGG + Chr2:24883482-24883501 MsG0280008056.01.T01:CDS 35.0%
CTTTCTTGAGAAAAAGAGGA+AGG + Chr2:24882912-24882931 MsG0280008056.01.T01:CDS 35.0%
GAAGATCTCTAATCAGATAG+TGG + Chr2:24882768-24882787 MsG0280008056.01.T01:CDS 35.0%
GAGAAAGTTATAGTTGAGGT+TGG + Chr2:24882943-24882962 MsG0280008056.01.T01:CDS 35.0%
GATAACTCAAGTGCAATTAG+GGG + Chr2:24883504-24883523 MsG0280008056.01.T01:CDS 35.0%
GATACACATGCAACAATAAG+GGG + Chr2:24883690-24883709 MsG0280008056.01.T01:CDS 35.0%
GCATCAATAAGAATCTGCAA+AGG - Chr2:24883828-24883847 None:intergenic 35.0%
GGAAGAGATTAATCTGAAGA+TGG + Chr2:24883242-24883261 MsG0280008056.01.T01:CDS 35.0%
GGATAACTCAAGTGCAATTA+GGG + Chr2:24883503-24883522 MsG0280008056.01.T01:CDS 35.0%
GGATACACATGCAACAATAA+GGG + Chr2:24883689-24883708 MsG0280008056.01.T01:CDS 35.0%
GTTTCAGTAGAACAACATTC+TGG + Chr2:24882649-24882668 MsG0280008056.01.T01:CDS 35.0%
TATATACCTGTCATGGAGAA+GGG + Chr2:24883207-24883226 MsG0280008056.01.T01:CDS 35.0%
TCAAACAATTCCACATTCGT+TGG - Chr2:24883336-24883355 None:intergenic 35.0%
TCAATAAGAATCTGCAAAGG+CGG - Chr2:24883825-24883844 None:intergenic 35.0%
TGCACTTGAGTTATCCTTAA+AGG - Chr2:24883499-24883518 None:intergenic 35.0%
TGTTTGGATTTGCCTGTAAA+GGG - Chr2:24883464-24883483 None:intergenic 35.0%
TTATATACCTGTCATGGAGA+AGG + Chr2:24883206-24883225 MsG0280008056.01.T01:CDS 35.0%
TTGGGGTAACCTTTGTAATA+GGG + Chr2:24882555-24882574 MsG0280008056.01.T01:CDS 35.0%
TTGTTTGGATTTGCCTGTAA+AGG - Chr2:24883465-24883484 None:intergenic 35.0%
TTTCTTGGATAAACGGTACA+CGG - Chr2:24882714-24882733 None:intergenic 35.0%
TTTGACAGAAGTGAATCGTT+TGG + Chr2:24883029-24883048 MsG0280008056.01.T01:CDS 35.0%
! ACATAATTTTCCATGTGCAG+TGG + Chr2:24883623-24883642 MsG0280008056.01.T01:CDS 35.0%
! AGTAGAACAACATTCTGGTT+TGG + Chr2:24882654-24882673 MsG0280008056.01.T01:CDS 35.0%
! CCCCAAAGCAATTGAATTTT+AGG - Chr2:24882541-24882560 None:intergenic 35.0%
! TTCAAAGGATGTTAGCTTCT+GGG - Chr2:24883160-24883179 None:intergenic 35.0%
! TTTCAAAGGATGTTAGCTTC+TGG - Chr2:24883161-24883180 None:intergenic 35.0%
! TTTGGGGTAACCTTTGTAAT+AGG + Chr2:24882554-24882573 MsG0280008056.01.T01:CDS 35.0%
!! GTTGATGGGGTTTATGATTT+TGG + Chr2:24882464-24882483 MsG0280008056.01.T01:intron 35.0%
!! TGCATCAGAGTCTTATTCAT+AGG - Chr2:24883429-24883448 None:intergenic 35.0%
!!! GGGTTTATGATTTTGGTTAC+AGG + Chr2:24882471-24882490 MsG0280008056.01.T01:intron 35.0%
AAACACGACCCCTATTACAA+AGG - Chr2:24882567-24882586 None:intergenic 40.0%
AGTAATGGTGCTTCTGACTA+CGG + Chr2:24883849-24883868 MsG0280008056.01.T01:CDS 40.0%
ATATACCTGTCATGGAGAAG+GGG + Chr2:24883208-24883227 MsG0280008056.01.T01:CDS 40.0%
CTACAGCTGGTTATTGTGTT+GGG + Chr2:24883730-24883749 MsG0280008056.01.T01:CDS 40.0%
CTCTCAGTTCCTAAATTCTC+AGG - Chr2:24882892-24882911 None:intergenic 40.0%
CTGTGGTAAATGATAACCTG+GGG - Chr2:24882969-24882988 None:intergenic 40.0%
CTTGAGAAAAAGAGGAAGGA+GGG + Chr2:24882916-24882935 MsG0280008056.01.T01:CDS 40.0%
GAAAGCTTTCGGTTCTTAGA+TGG + Chr2:24882845-24882864 MsG0280008056.01.T01:CDS 40.0%
GCTGCACTTTCTTGGATAAA+CGG - Chr2:24882721-24882740 None:intergenic 40.0%
GCTGTGGTAAATGATAACCT+GGG - Chr2:24882970-24882989 None:intergenic 40.0%
GGGATACACATGCAACAATA+AGG + Chr2:24883688-24883707 MsG0280008056.01.T01:CDS 40.0%
GGTTATCATTTACCACAGCA+TGG + Chr2:24882971-24882990 MsG0280008056.01.T01:CDS 40.0%
TAAGCGTAATCCAACGAATG+TGG + Chr2:24883323-24883342 MsG0280008056.01.T01:CDS 40.0%
TCAAGAAAATCCAAGAGCTG+TGG + Chr2:24882627-24882646 MsG0280008056.01.T01:CDS 40.0%
TCGCGTTCTGTCAAGTTTAA+CGG - Chr2:24883576-24883595 None:intergenic 40.0%
TCTGACTACGGGAACAAATT+TGG + Chr2:24883861-24883880 MsG0280008056.01.T01:CDS 40.0%
TCTTGAGAAAAAGAGGAAGG+AGG + Chr2:24882915-24882934 MsG0280008056.01.T01:CDS 40.0%
TGCTGTGGTAAATGATAACC+TGG - Chr2:24882971-24882990 None:intergenic 40.0%
TGGGGTAACCTTTGTAATAG+GGG + Chr2:24882556-24882575 MsG0280008056.01.T01:CDS 40.0%
TGGTGTAGTATTCCAAATCC+TGG - Chr2:24883284-24883303 None:intergenic 40.0%
TTGGCAAATTGACACAGCAT+TGG - Chr2:24883001-24883020 None:intergenic 40.0%
TTGGGAATTTCAACACAGCA+GGG + Chr2:24883748-24883767 MsG0280008056.01.T01:CDS 40.0%
! CAATGCTTTAATGTTGGCCT+TGG + Chr2:24882805-24882824 MsG0280008056.01.T01:CDS 40.0%
! CCTTTCAAGTGGAAAGCTTT+CGG + Chr2:24882834-24882853 MsG0280008056.01.T01:CDS 40.0%
! TGCATTTGATGACCAGGATT+TGG + Chr2:24883269-24883288 MsG0280008056.01.T01:CDS 40.0%
!! CTTTGAAACCAGTGTAGTTC+CGG + Chr2:24883173-24883192 MsG0280008056.01.T01:CDS 40.0%
!! GTTTAACGGACTTGTTGAAC+CGG - Chr2:24883562-24883581 None:intergenic 40.0%
!! TTGAGCCTGCTTTAAAAGCT+CGG - Chr2:24882746-24882765 None:intergenic 40.0%
!! TTTAACGGACTTGTTGAACC+GGG - Chr2:24883561-24883580 None:intergenic 40.0%
!!! AGCAGGCATTGGTTTTTTAC+CGG + Chr2:24883381-24883400 MsG0280008056.01.T01:CDS 40.0%
AAATTCCTCCGGAACTACAC+TGG - Chr2:24883184-24883203 None:intergenic 45.0%
ATAAAGTCGAACGAACCGGA+AGG - Chr2:24882392-24882411 None:intergenic 45.0%
CCAAAGTTCTACAGAAGCCT+TGG - Chr2:24883899-24883918 None:intergenic 45.0%
CCAAGGCTTCTGTAGAACTT+TGG + Chr2:24883896-24883915 MsG0280008056.01.T01:CDS 45.0%
CCAAGTAAAGCCATTACGAC+CGG + Chr2:24883530-24883549 MsG0280008056.01.T01:CDS 45.0%
CCGAAAGCTTTCCACTTGAA+AGG - Chr2:24882837-24882856 None:intergenic 45.0%
CTACAGAAGCCTTGGATCAA+CGG - Chr2:24883891-24883910 None:intergenic 45.0%
CTTCTGTCAAACCACATGCT+TGG - Chr2:24883020-24883039 None:intergenic 45.0%
CTTGGTTCACACCTTTCAAG+TGG + Chr2:24882823-24882842 MsG0280008056.01.T01:CDS 45.0%
GAAAAAGAGGAAGGAGGGTT+TGG + Chr2:24882921-24882940 MsG0280008056.01.T01:CDS 45.0%
GCTACAGCTGGTTATTGTGT+TGG + Chr2:24883729-24883748 MsG0280008056.01.T01:CDS 45.0%
GTAATGGTGCTTCTGACTAC+GGG + Chr2:24883850-24883869 MsG0280008056.01.T01:CDS 45.0%
GTGTCAATTTGCCAAGCATG+TGG + Chr2:24883006-24883025 MsG0280008056.01.T01:CDS 45.0%
GTTGGGAATTTCAACACAGC+AGG + Chr2:24883747-24883766 MsG0280008056.01.T01:CDS 45.0%
TCTACTGAAACCACAGCTCT+TGG - Chr2:24882640-24882659 None:intergenic 45.0%
TTGCAGCTATGGTGCATGAT+AGG + Chr2:24883115-24883134 MsG0280008056.01.T01:CDS 45.0%
! AAAGGCGGTGCCAAATTTGA+TGG - Chr2:24883810-24883829 None:intergenic 45.0%
! CACTTGAAAGGTGTGAACCA+AGG - Chr2:24882825-24882844 None:intergenic 45.0%
! TGAATCGTTTGGAGCGTTCA+AGG + Chr2:24883040-24883059 MsG0280008056.01.T01:CDS 45.0%
!! CGGAACTACACTGGTTTCAA+AGG - Chr2:24883175-24883194 None:intergenic 45.0%
!! GGGTTTTGCATTTGATGACC+AGG + Chr2:24883263-24883282 MsG0280008056.01.T01:CDS 45.0%
ACAGTGAGCATAGCAGGCAT+TGG + Chr2:24883370-24883389 MsG0280008056.01.T01:CDS 50.0%
AGTCCAACAGTGAGCATAGC+AGG + Chr2:24883364-24883383 MsG0280008056.01.T01:CDS 50.0%
ATGCCTGCTATGCTCACTGT+TGG - Chr2:24883370-24883389 None:intergenic 50.0%
ATGGCAGCTGCTGGAGAAAT+TGG + Chr2:24882354-24882373 MsG0280008056.01.T01:CDS 50.0%
CGAACGAACCGGAAGGAATT+CGG - Chr2:24882385-24882404 None:intergenic 50.0%
GTTAAGGACGGATCTTCCCA+TGG - Chr2:24883783-24883802 None:intergenic 50.0%
TGAAACCAGTGTAGTTCCGG+AGG + Chr2:24883176-24883195 MsG0280008056.01.T01:CDS 50.0%
TGGCAGCTGCTGGAGAAATT+GGG + Chr2:24882355-24882374 MsG0280008056.01.T01:CDS 50.0%
TGTGCAGTGGTACCTTATCC+CGG + Chr2:24883636-24883655 MsG0280008056.01.T01:CDS 50.0%
TTTGGAGAGCCGTTGATCCA+AGG + Chr2:24883879-24883898 MsG0280008056.01.T01:CDS 50.0%
TTTGTGCAAGCAGCTACAGC+TGG + Chr2:24883717-24883736 MsG0280008056.01.T01:CDS 50.0%
! ATCGTTTGGAGCGTTCAAGG+AGG + Chr2:24883043-24883062 MsG0280008056.01.T01:CDS 50.0%
! CCGGTCGTAATGGCTTTACT+TGG - Chr2:24883533-24883552 None:intergenic 50.0%
! GGATAAGGTACCACTGCACA+TGG - Chr2:24883636-24883655 None:intergenic 50.0%
! TGGGGTATCCGAATTCCTTC+CGG + Chr2:24882374-24882393 MsG0280008056.01.T01:CDS 50.0%
! TTATAGTTGAGGTTGGCCCC+AGG + Chr2:24882950-24882969 MsG0280008056.01.T01:CDS 50.0%
!!! AGCTGCCGAGCTTTTAAAGC+AGG + Chr2:24882738-24882757 MsG0280008056.01.T01:CDS 50.0%
AGCAGGGCTCTATGCTCCAT+GGG + Chr2:24883764-24883783 MsG0280008056.01.T01:CDS 55.0%
AGCTGCAGGAGGTCGCATTA+GGG + Chr2:24883668-24883687 MsG0280008056.01.T01:CDS 55.0%
CTTGTTGAACCGGGCTGAAC+CGG - Chr2:24883552-24883571 None:intergenic 55.0%
GGCAGCTGCTGGAGAAATTG+GGG + Chr2:24882356-24882375 MsG0280008056.01.T01:CDS 55.0%
TACCTGTCATGGAGAAGGGG+AGG + Chr2:24883211-24883230 MsG0280008056.01.T01:CDS 55.0%
TGTCATGGAGAAGGGGAGGA+AGG + Chr2:24883215-24883234 MsG0280008056.01.T01:CDS 55.0%
TTCCTCCCCTTCTCCATGAC+AGG - Chr2:24883216-24883235 None:intergenic 55.0%
!! AGCATTGGCAGACCATGCTG+TGG - Chr2:24882986-24883005 None:intergenic 55.0%
CAGCAGGGCTCTATGCTCCA+TGG + Chr2:24883763-24883782 MsG0280008056.01.T01:CDS 60.0%
CAGCTGCAGGAGGTCGCATT+AGG + Chr2:24883667-24883686 MsG0280008056.01.T01:CDS 60.0%
GCTCGGCAGCTGCACTTTCT+TGG - Chr2:24882729-24882748 None:intergenic 60.0%
GCTGTCTCTGCACCGGGATA+AGG - Chr2:24883651-24883670 None:intergenic 60.0%
TCCTGCAGCTGTCTCTGCAC+CGG - Chr2:24883658-24883677 None:intergenic 60.0%
! CCATTACGACCGGTTCAGCC+CGG + Chr2:24883540-24883559 MsG0280008056.01.T01:CDS 60.0%
! GGAGAAGGGGAGGAAGGCAT+TGG + Chr2:24883221-24883240 MsG0280008056.01.T01:CDS 60.0%
CCGGGCTGAACCGGTCGTAA+TGG - Chr2:24883543-24883562 None:intergenic 65.0%
CCTGCAGCTGTCTCTGCACC+GGG - Chr2:24883657-24883676 None:intergenic 65.0%
GGTGCAGAGACAGCTGCAGG+AGG + Chr2:24883657-24883676 MsG0280008056.01.T01:CDS 65.0%
CCCGGTGCAGAGACAGCTGC+AGG + Chr2:24883654-24883673 MsG0280008056.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 24882354 24883923 24882354 ID=MsG0280008056.01;Name=MsG0280008056.01
Chr2 mRNA 24882354 24883923 24882354 ID=MsG0280008056.01.T01;Parent=MsG0280008056.01;Name=MsG0280008056.01.T01;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|1|1|2|0|490
Chr2 exon 24882354 24882395 24882354 ID=MsG0280008056.01.T01:exon:34842;Parent=MsG0280008056.01.T01
Chr2 exon 24882493 24883923 24882493 ID=MsG0280008056.01.T01:exon:34843;Parent=MsG0280008056.01.T01
Chr2 CDS 24882354 24882395 24882354 ID=MsG0280008056.01.T01:cds;Parent=MsG0280008056.01.T01
Chr2 CDS 24882493 24883923 24882493 ID=MsG0280008056.01.T01:cds;Parent=MsG0280008056.01.T01
Gene Sequence

>MsG0280008056.01.T01

ATGGCAGCTGCTGGAGAAATTGGGGTATCCGAATTCCTTCCGGGTACATGCAGACAAACATTGTTTTTGCTAAAGAAACCTCAGAAACCTAAAATTCAATTGCTTTGGGGTAACCTTTGTAATAGGGGTCGTGTTTCAAGTTCATCTCGAAAATCGTTGAGTTTGAGATGTCAAGCTCAAGAAAATCCAAGAGCTGTGGTTTCAGTAGAACAACATTCTGGTTTGGTTGAGAAGCCTTCTGCTCAAGTTATTCATCTGTACCGTGTACCGTTTATCCAAGAAAGTGCAGCTGCCGAGCTTTTAAAGCAGGCTCAAGCGAAGATCTCTAATCAGATAGTGGACTTAAAGACTGAACAATGCTTTAATGTTGGCCTTGGTTCACACCTTTCAAGTGGAAAGCTTTCGGTTCTTAGATGGCTTCTTTCAGAAACATTTGAGCCTGAGAATTTAGGAACTGAGAGCTTTCTTGAGAAAAAGAGGAAGGAGGGTTTGGAGAAAGTTATAGTTGAGGTTGGCCCCAGGTTATCATTTACCACAGCATGGTCTGCCAATGCTGTGTCAATTTGCCAAGCATGTGGTTTGACAGAAGTGAATCGTTTGGAGCGTTCAAGGAGGTATTTGTTGTATACAAATGGTGAACTACAAGAACATCAAATTAATGAATTTGCAGCTATGGTGCATGATAGGATGACTGAATGCGTTTATACCCAGAAGCTAACATCCTTTGAAACCAGTGTAGTTCCGGAGGAATTTTATTATATACCTGTCATGGAGAAGGGGAGGAAGGCATTGGAAGAGATTAATCTGAAGATGGGTTTTGCATTTGATGACCAGGATTTGGAATACTACACCAAACTTTTCAGAGAAGATACTAAGCGTAATCCAACGAATGTGGAATTGTTTGATATTGCTCAGTCCAACAGTGAGCATAGCAGGCATTGGTTTTTTACCGGAAAGATTTTTATTGACGGACAACCTATGAATAAGACTCTGATGCAAATTGTGAAAAGCCCTTTACAGGCAAATCCAAACAACTCAGTTATTGCCTTTAAGGATAACTCAAGTGCAATTAGGGGTTTCCAAGTAAAGCCATTACGACCGGTTCAGCCCGGTTCAACAAGTCCGTTAAACTTGACAGAACGCGACATGAATATCTTATTTACAGCTGAAACACATAATTTTCCATGTGCAGTGGTACCTTATCCCGGTGCAGAGACAGCTGCAGGAGGTCGCATTAGGGATACACATGCAACAATAAGGGGTTCGTTTGTGCAAGCAGCTACAGCTGGTTATTGTGTTGGGAATTTCAACACAGCAGGGCTCTATGCTCCATGGGAAGATCCGTCCTTAACTTATCCATCAAATTTGGCACCGCCTTTGCAGATTCTTATTGATGCAAGTAATGGTGCTTCTGACTACGGGAACAAATTTGGAGAGCCGTTGATCCAAGGCTTCTGTAGAACTTTGGAATGA
Protein sequence

>MsG0280008056.01.T01

MAAAGEIGVSEFLPGTCRQTLFLLKKPQKPKIQLLWGNLCNRGRVSSSSRKSLSLRCQAQENPRAVVSVEQHSGLVEKPSAQVIHLYRVPFIQESAAAELLKQAQAKISNQIVDLKTEQCFNVGLGSHLSSGKLSVLRWLLSETFEPENLGTESFLEKKRKEGLEKVIVEVGPRLSFTTAWSANAVSICQACGLTEVNRLERSRRYLLYTNGELQEHQINEFAAMVHDRMTECVYTQKLTSFETSVVPEEFYYIPVMEKGRKALEEINLKMGFAFDDQDLEYYTKLFREDTKRNPTNVELFDIAQSNSEHSRHWFFTGKIFIDGQPMNKTLMQIVKSPLQANPNNSVIAFKDNSSAIRGFQVKPLRPVQPGSTSPLNLTERDMNILFTAETHNFPCAVVPYPGAETAAGGRIRDTHATIRGSFVQAATAGYCVGNFNTAGLYAPWEDPSLTYPSNLAPPLQILIDASNGASDYGNKFGEPLIQGFCRTLE*