AlfalfaGEDB | MsG0280008580.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008580.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008580.01.T01	ABN08053.1	68.142	113	36	0	181	293	4	116	2.66E-46	162

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008580.01.T01	A2Q353	68.142	113	36	0	181	293	4	116	1.27e-46	162

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280008580.01	MsG0380013342.01	-0.804584	2.014358e-49	1.042508e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008580.01.T01	MTR_4g132870	44.444	99	51	1	199	293	78	176	1.71e-21	92.0
MsG0280008580.01.T01	MTR_4g132870	35.938	128	74	3	41	160	50	177	2.76e-14	72.0
MsG0280008580.01.T01	MTR_4g055460	49.296	71	36	0	198	268	161	231	1.15e-16	78.2
MsG0280008580.01.T01	MTR_4g055460	33.898	118	77	1	36	152	136	253	3.19e-12	65.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 85 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGAAGGAGAAATTGA+GGG	0.587362	2:-34287687	None:intergenic
AAATCGATGAAGAAGAAAGG+GGG	0.627021	2:-34287640	None:intergenic
AACAACACCGACAATATTCT+AGG	0.331954	2:-34290765	None:intergenic
AACCGGATACTGGCAGTGAC+CGG	0.491295	2:-34289752	None:intergenic
AATCATTGTTGAAGTCACAA+TGG	0.620109	2:-34290736	None:intergenic
AATCGATGAAGAAGAAAGGG+GGG	0.613243	2:-34287639	None:intergenic
ACAATGCTATTGGCCCCTAT+TGG	0.393947	2:+34290598	MsG0280008580.01.T01:CDS
ACACATAGCGACTTTCCAAT+AGG	0.371367	2:-34290613	None:intergenic
ACATAGCGACTTTCCAATAG+GGG	0.641769	2:-34290611	None:intergenic
ACCAAGAGGACTTACATGGA+CGG	0.661202	2:-34288719	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGTGAAGGAGAAATTG+AGG	-	Chr2:34287691-34287710	None:intergenic	30.0%
AAAAGTGAAGGAGAAATTGA+GGG	-	Chr2:34287690-34287709	None:intergenic	30.0%
AAACTACCACGAATAATCAA+AGG	-	Chr2:34288649-34288668	None:intergenic	30.0%
AATCATTGTTGAAGTCACAA+TGG	-	Chr2:34290739-34290758	None:intergenic	30.0%
GAAATTGAGGGTTAAATTGA+AGG	-	Chr2:34287678-34287697	None:intergenic	30.0%
GCAAATTTCAGTTAAAGCAT+GGG	+	Chr2:34289899-34289918	MsG0280008580.01.T01:CDS	30.0%
TAAAAGCTACGCAGATTATA+AGG	+	Chr2:34289576-34289595	MsG0280008580.01.T01:CDS	30.0%
TCTTACGTTATGGTTTGATT+CGG	+	Chr2:34289290-34289309	MsG0280008580.01.T01:intron	30.0%
TGTATGTATGGAAATATACC+TGG	-	Chr2:34288525-34288544	None:intergenic	30.0%
TTCTTCTTGTTAGCCAATAA+AGG	-	Chr2:34288698-34288717	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	34287598	34290898	34287598	ID=MsG0280008580.01;Name=MsG0280008580.01
Chr2	mRNA	34287598	34290898	34287598	ID=MsG0280008580.01.T01;Parent=MsG0280008580.01;Name=MsG0280008580.01.T01;_AED=0.42;_eAED=0.42;_QI=130\|0.66\|1\|1\|1\|1\|4\|179\|296
Chr2	exon	34287598	34287761	34287598	ID=MsG0280008580.01.T01:exon:15201;Parent=MsG0280008580.01.T01
Chr2	exon	34288666	34288726	34288666	ID=MsG0280008580.01.T01:exon:15202;Parent=MsG0280008580.01.T01
Chr2	exon	34289549	34289962	34289549	ID=MsG0280008580.01.T01:exon:15203;Parent=MsG0280008580.01.T01
Chr2	exon	34290338	34290898	34290338	ID=MsG0280008580.01.T01:exon:15204;Parent=MsG0280008580.01.T01
Chr2	five_prime_UTR	34287598	34287727	34287598	ID=MsG0280008580.01.T01:five_prime_utr;Parent=MsG0280008580.01.T01
Chr2	CDS	34287728	34287761	34287728	ID=MsG0280008580.01.T01:cds;Parent=MsG0280008580.01.T01
Chr2	CDS	34288666	34288726	34288666	ID=MsG0280008580.01.T01:cds;Parent=MsG0280008580.01.T01
Chr2	CDS	34289549	34289962	34289549	ID=MsG0280008580.01.T01:cds;Parent=MsG0280008580.01.T01
Chr2	CDS	34290338	34290719	34290338	ID=MsG0280008580.01.T01:cds;Parent=MsG0280008580.01.T01
Chr2	three_prime_UTR	34290720	34290898	34290720	ID=MsG0280008580.01.T01:three_prime_utr;Parent=MsG0280008580.01.T01

Gene Sequence

>MsG0280008580.01.T01

ATGGAACAAACCCTCTTCTCACAGTCGTCTCCAGTTTTTAGCTTGAAGGTGAATCATGTTCCTCCTTTATTGGCTAACAAGAAGAAGCCGTCCATGAAGGGTCACCGGAGTCGTGCGCCTGTTAAAAGCTACGCAGATTATAAGGTTAAGCTTAATGATTATGTAGAAAATAATGTTGGACCAGTCTCGTTCATTGTAAACCATCGCTTGGGTCGAGCGCACTACGAACGTATTTTGTATGATCTACGCCTTAGCAACGTAATGTTGGGTCGACGTGACAAAATTAAAAATCCAAGACCCGGTCACTGCCAGTATCCGGTTGTTTGTTCCTCCGTAGAGGTTATGAAAGCTGCTTACAATCTTTTTGGCCGTCATTGGAAGGTCATTATGCGTTCACGACCAGATTATGGGGATTGCAAGAAAGCGAAACTATTGGCTGAAATGCGCAAATTTCAGTTAAAGCATGGGATTGATATTCATTCTACCGCTCAGCCGTCCAGGAAGAAAAGCACAAAGACTCGAGTGAATGTTTCAGTTACAAGCTTGGATGATTTTCTGGATTCGCTTCAGCAGAACATTAATAATGCTGACCCAGTCTCTTTCAATGTTTCCAAGGACGTGGATACAGAATCCTATGGTGAAATTGTGGGCGCTCTACTTGCTAGCAACACTCGGTGGAATCGATCTGACAAAATCAAACATCCAAGATCCGATCACTTCCCGCCACCGGTCGTGCATTGTGAAACAACAGAAAAGATGAAAAATGTTTACAATGCTATTGGCCCCTATTGGAAAGTCGCTATGTGTGTGTGTGAACATGACAACCCTAAGCTATTTTATCAAATGGACGAGTATCGGATGACTGCTGGGATTGAAGACTGCTGGGATTGA

Protein sequence

>MsG0280008580.01.T01

MEQTLFSQSSPVFSLKVNHVPPLLANKKKPSMKGHRSRAPVKSYADYKVKLNDYVENNVGPVSFIVNHRLGRAHYERILYDLRLSNVMLGRRDKIKNPRPGHCQYPVVCSSVEVMKAAYNLFGRHWKVIMRSRPDYGDCKKAKLLAEMRKFQLKHGIDIHSTAQPSRKKSTKTRVNVSVTSLDDFLDSLQQNINNADPVSFNVSKDVDTESYGEIVGALLASNTRWNRSDKIKHPRSDHFPPPVVHCETTEKMKNVYNAIGPYWKVAMCVCEHDNPKLFYQMDEYRMTAGIEDCWD*