AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008580.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008580.01.T01 MTR_4g132870 44.444 99 51 1 199 293 78 176 1.71e-21 92.0
MsG0280008580.01.T01 MTR_4g132870 35.938 128 74 3 41 160 50 177 2.76e-14 72.0
MsG0280008580.01.T01 MTR_4g055460 49.296 71 36 0 198 268 161 231 1.15e-16 78.2
MsG0280008580.01.T01 MTR_4g055460 33.898 118 77 1 36 152 136 253 3.19e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 85 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCATGTTCCTCCTTTAT+TGG 0.199140 2:+34288682 MsG0280008580.01.T01:CDS
TGGTGTTTATTACTTTATTT+TGG 0.206149 2:+34290838 MsG0280008580.01.T01:three_prime_UTR
ATGCGTTCACGACCAGATTA+TGG 0.218291 2:+34289841 MsG0280008580.01.T01:CDS
TTCTTCTTGTTAGCCAATAA+AGG 0.307199 2:-34288695 None:intergenic
TAAAAGCTACGCAGATTATA+AGG 0.314361 2:+34289576 MsG0280008580.01.T01:CDS
AACAACACCGACAATATTCT+AGG 0.331954 2:-34290765 None:intergenic
AGAAGAGGGTTTGTTCCATT+TGG 0.349194 2:-34287724 None:intergenic
ACACATAGCGACTTTCCAAT+AGG 0.371367 2:-34290613 None:intergenic
TACTGGCAGTGACCGGGTCT+TGG 0.373908 2:-34289745 None:intergenic
TGCGTTCACGACCAGATTAT+GGG 0.383310 2:+34289842 MsG0280008580.01.T01:CDS
GACGAGTATCGGATGACTGC+TGG 0.391157 2:+34290675 MsG0280008580.01.T01:CDS
TCCATTTGATAAAATAGCTT+AGG 0.393197 2:-34290654 None:intergenic
ACAATGCTATTGGCCCCTAT+TGG 0.393947 2:+34290598 MsG0280008580.01.T01:CDS
TTCTTGCAATCCCCATAATC+TGG 0.405714 2:-34289853 None:intergenic
CACATAGCGACTTTCCAATA+GGG 0.420212 2:-34290612 None:intergenic
AGGAACAAACAACCGGATAC+TGG 0.429199 2:-34289762 None:intergenic
ATCAATAAGTTGCAAAGATA+AGG 0.430769 2:-34290883 None:intergenic
TCACAATGCACGACCGGTGG+CGG 0.436165 2:-34290549 None:intergenic
CGCAAATTTCAGTTAAAGCA+TGG 0.442371 2:+34289898 MsG0280008580.01.T01:CDS
CCATTTGATAAAATAGCTTA+GGG 0.443144 2:-34290653 None:intergenic
GAAATTGAGGGTTAAATTGA+AGG 0.445159 2:-34287675 None:intergenic
TTGTCGGTGTTGTTAATCAA+TGG 0.448283 2:+34290774 MsG0280008580.01.T01:three_prime_UTR
TTGGAAACATTGAAAGAGAC+TGG 0.449745 2:-34290420 None:intergenic
GTTCATTGTAAACCATCGCT+TGG 0.462263 2:+34289642 MsG0280008580.01.T01:CDS
CTGCTGGGATTGAAGACTGC+TGG 0.462469 2:+34290691 MsG0280008580.01.T01:CDS
TTCACGAAGAAGTCTCCAAA+TGG 0.463065 2:+34287709 MsG0280008580.01.T01:exon
GGCGGGAAGTGATCGGATCT+TGG 0.464471 2:-34290531 None:intergenic
CGCCTTAGCAACGTAATGTT+GGG 0.468300 2:+34289700 MsG0280008580.01.T01:CDS
GGATTCTGTATCCACGTCCT+TGG 0.470569 2:-34290439 None:intergenic
GAATGTTTCAGTTACAAGCT+TGG 0.472320 2:+34290353 MsG0280008580.01.T01:CDS
TTAGAATCCTAGAATATTGT+CGG 0.473995 2:+34290758 MsG0280008580.01.T01:three_prime_UTR
ACCCGGTCACTGCCAGTATC+CGG 0.474365 2:+34289750 MsG0280008580.01.T01:CDS
TGCTGGGATTGAAGACTGCT+GGG 0.476407 2:+34290692 MsG0280008580.01.T01:CDS
CTCTTCTCACAGTCGTCTCC+AGG 0.476908 2:+34287740 MsG0280008580.01.T01:CDS
TTCATTGTAAACCATCGCTT+GGG 0.480273 2:+34289643 MsG0280008580.01.T01:CDS
TTGCAAGAAAGCGAAACTAT+TGG 0.482845 2:+34289867 MsG0280008580.01.T01:CDS
ATTCTACCGCTCAGCCGTCC+AGG 0.484945 2:+34289932 MsG0280008580.01.T01:CDS
GGAAATCGATGAAGAAGAAA+GGG 0.490353 2:-34287642 None:intergenic
AACCGGATACTGGCAGTGAC+CGG 0.491295 2:-34289752 None:intergenic
AGGAAATCGATGAAGAAGAA+AGG 0.494355 2:-34287643 None:intergenic
AGAATCCTATGGTGAAATTG+TGG 0.496872 2:+34290455 MsG0280008580.01.T01:CDS
CTCAGCCGTCCAGGAAGAAA+AGG 0.496905 2:+34289941 MsG0280008580.01.T01:CDS
CACAATGCACGACCGGTGGC+GGG 0.499197 2:-34290548 None:intergenic
TTTATCAAATGGACGAGTAT+CGG 0.499777 2:+34290664 MsG0280008580.01.T01:CDS
CTCTACTTGCTAGCAACACT+CGG 0.507817 2:+34290481 MsG0280008580.01.T01:CDS
GACCGGTGGCGGGAAGTGAT+CGG 0.510423 2:-34290538 None:intergenic
TTTCAATGTTTCCAAGGACG+TGG 0.513334 2:+34290428 MsG0280008580.01.T01:CDS
GAATCCTATGGTGAAATTGT+GGG 0.518389 2:+34290456 MsG0280008580.01.T01:CDS
GTTGTTTCACAATGCACGAC+CGG 0.518585 2:-34290555 None:intergenic
ATCCGATCACTTCCCGCCAC+CGG 0.523056 2:+34290536 MsG0280008580.01.T01:CDS
GCAAATTTCAGTTAAAGCAT+GGG 0.529716 2:+34289899 MsG0280008580.01.T01:CDS
ACGCCTTAGCAACGTAATGT+TGG 0.531847 2:+34289699 MsG0280008580.01.T01:CDS
TCTACGGAGGAACAAACAAC+CGG 0.539052 2:-34289769 None:intergenic
GATTATGTAGAAAATAATGT+TGG 0.546250 2:+34289610 MsG0280008580.01.T01:CDS
AGTCTCTTTCAATGTTTCCA+AGG 0.561721 2:+34290422 MsG0280008580.01.T01:CDS
ACGAGTATCGGATGACTGCT+GGG 0.571376 2:+34290676 MsG0280008580.01.T01:CDS
AGCAGCTTTCATAACCTCTA+CGG 0.576487 2:-34289785 None:intergenic
GACCCAACATTACGTTGCTA+AGG 0.579421 2:-34289702 None:intergenic
ACCGGATACTGGCAGTGACC+GGG 0.581274 2:-34289751 None:intergenic
GCATAATGACCTTCCAATGA+CGG 0.585919 2:-34289822 None:intergenic
TGGTTTACAATGAACGAGAC+TGG 0.586508 2:-34289634 None:intergenic
AAAAGTGAAGGAGAAATTGA+GGG 0.587362 2:-34287687 None:intergenic
CTTAGTACTAGTCTCATGCC+AGG 0.588092 2:-34290802 None:intergenic
CTGGAGACGACTGTGAGAAG+AGG 0.589616 2:-34287739 None:intergenic
GGTTGTTTGTTCCTCCGTAG+AGG 0.596219 2:+34289771 MsG0280008580.01.T01:CDS
TGTTAATCAATGGAGTGGCC+TGG 0.596485 2:+34290784 MsG0280008580.01.T01:three_prime_UTR
TGGAGACGACTGTGAGAAGA+GGG 0.598704 2:-34287738 None:intergenic
AGCGCCCACAATTTCACCAT+AGG 0.600976 2:-34290460 None:intergenic
AGTGCGCTCGACCCAAGCGA+TGG 0.611833 2:-34289654 None:intergenic
ACTTATGTAGGAAGGGTCAC+CGG 0.612125 2:+34289539 MsG0280008580.01.T01:intron
AATCGATGAAGAAGAAAGGG+GGG 0.613243 2:-34287639 None:intergenic
GTTTCACAATGCACGACCGG+TGG 0.615869 2:-34290552 None:intergenic
TTCTTGTTAGCCAATAAAGG+AGG 0.618073 2:-34288692 None:intergenic
GCGTTCACGACCAGATTATG+GGG 0.620043 2:+34289843 MsG0280008580.01.T01:CDS
AATCATTGTTGAAGTCACAA+TGG 0.620109 2:-34290736 None:intergenic
AAATCGATGAAGAAGAAAGG+GGG 0.627021 2:-34287640 None:intergenic
GAAATCGATGAAGAAGAAAG+GGG 0.637830 2:-34287641 None:intergenic
ACATAGCGACTTTCCAATAG+GGG 0.641769 2:-34290611 None:intergenic
GACGTGGATACAGAATCCTA+TGG 0.647023 2:+34290444 MsG0280008580.01.T01:CDS
TGGAAACATTGAAAGAGACT+GGG 0.660286 2:-34290419 None:intergenic
ACCAAGAGGACTTACATGGA+CGG 0.661202 2:-34288719 None:intergenic
TTTCTTCCTGGACGGCTGAG+CGG 0.667301 2:-34289938 None:intergenic
TACTTGCTAGCAACACTCGG+TGG 0.678846 2:+34290484 MsG0280008580.01.T01:CDS
AGCTTTCATAACCTCTACGG+AGG 0.687877 2:-34289782 None:intergenic
GGTGTTGTTAATCAATGGAG+TGG 0.688317 2:+34290779 MsG0280008580.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATAATAAACGATAA+TGG - Chr2:34290106-34290125 None:intergenic 10.0%
!!! TATTTTATGTTTTAATTTGT+TGG + Chr2:34290232-34290251 MsG0280008580.01.T01:intron 10.0%
!! AAAAAAGAACAATGTATAAA+TGG - Chr2:34288760-34288779 None:intergenic 15.0%
!! AAAAAGAAGAATGTAATATA+TGG - Chr2:34288394-34288413 None:intergenic 15.0%
!! AATAATAAAGAAAGAATTTC+AGG - Chr2:34287997-34288016 None:intergenic 15.0%
!! TTTAATTATGAAAATGAGTT+TGG + Chr2:34290126-34290145 MsG0280008580.01.T01:intron 15.0%
!!! TTTTTTTTTTTGTTCTGAAT+CGG - Chr2:34288361-34288380 None:intergenic 15.0%
!! AAAAATGTTTACAATGCTAT+TGG + Chr2:34290588-34290607 MsG0280008580.01.T01:CDS 20.0%
!! AAAGAGAAAGAAAACATATA+TGG - Chr2:34287919-34287938 None:intergenic 20.0%
!! ATGAAAACAAATGAAAACAA+GGG - Chr2:34289518-34289537 None:intergenic 20.0%
!! ATTGTATCATCTCAAAATAA+CGG - Chr2:34288953-34288972 None:intergenic 20.0%
!! GATTATGTAGAAAATAATGT+TGG + Chr2:34289610-34289629 MsG0280008580.01.T01:CDS 20.0%
!! TAAATTGAAGGAAAAAGAAA+AGG - Chr2:34287666-34287685 None:intergenic 20.0%
!! TATATGAATGTATGTATGTA+TGG - Chr2:34287769-34287788 None:intergenic 20.0%
!! TATGAAAACAAATGAAAACA+AGG - Chr2:34289519-34289538 None:intergenic 20.0%
!! TGAAAATGAAATTGTAATGT+TGG - Chr2:34289061-34289080 None:intergenic 20.0%
!!! ACATACAAGGTTTTAAATAT+TGG + Chr2:34290045-34290064 MsG0280008580.01.T01:intron 20.0%
!!! ATTAGATGTATTTTGTTATG+TGG + Chr2:34289997-34290016 MsG0280008580.01.T01:intron 20.0%
!!! CTTGTTTTTGTATATAGTTT+TGG + Chr2:34288161-34288180 MsG0280008580.01.T01:intron 20.0%
!!! TACTAAGTTTCTTTTCAATT+TGG + Chr2:34290818-34290837 MsG0280008580.01.T01:three_prime_UTR 20.0%
!!! TCATTTTTTCCTTTGATTTT+TGG + Chr2:34287881-34287900 MsG0280008580.01.T01:intron 20.0%
!!! TCTTAGTCTTCTTATAAAAT+AGG - Chr2:34289139-34289158 None:intergenic 20.0%
!!! TGCATAAATTTGATAACAAA+TGG - Chr2:34290317-34290336 None:intergenic 20.0%
!!! TGGTGTTTATTACTTTATTT+TGG + Chr2:34290838-34290857 MsG0280008580.01.T01:three_prime_UTR 20.0%
!!! TTTGTTTTCATAACTTATGT+AGG + Chr2:34289527-34289546 MsG0280008580.01.T01:intron 20.0%
!!! TTTTTGTATATAGTTTTGGT+AGG + Chr2:34288165-34288184 MsG0280008580.01.T01:intron 20.0%
! AAAATTAAAAATCCAAGACC+CGG + Chr2:34289733-34289752 MsG0280008580.01.T01:CDS 25.0%
! CCATTTGATAAAATAGCTTA+GGG - Chr2:34290656-34290675 None:intergenic 25.0%
! GATTGAGTTTCTATAAGTAT+TGG + Chr2:34289264-34289283 MsG0280008580.01.T01:intron 25.0%
! TCATTAGATAGTTACATACA+AGG + Chr2:34290032-34290051 MsG0280008580.01.T01:intron 25.0%
! TCCATTTGATAAAATAGCTT+AGG - Chr2:34290657-34290676 None:intergenic 25.0%
! TGTATGTATGTAAATATACC+TGG - Chr2:34287761-34287780 None:intergenic 25.0%
! TTAGAATCCTAGAATATTGT+CGG + Chr2:34290758-34290777 MsG0280008580.01.T01:three_prime_UTR 25.0%
! TTTCAAACTTAATAAGCCTT+TGG + Chr2:34289478-34289497 MsG0280008580.01.T01:intron 25.0%
! TTTCATAACTTATGTAGGAA+GGG + Chr2:34289532-34289551 MsG0280008580.01.T01:intron 25.0%
!! GATTCGATTTTCATTTCTTT+TGG + Chr2:34289419-34289438 MsG0280008580.01.T01:intron 25.0%
!! GATTTTCATTTCTTTTGGAT+TGG + Chr2:34289424-34289443 MsG0280008580.01.T01:intron 25.0%
!! TTTGAAACGAATCAAAAGTA+GGG - Chr2:34289465-34289484 None:intergenic 25.0%
!! TTTTCATAACTTATGTAGGA+AGG + Chr2:34289531-34289550 MsG0280008580.01.T01:intron 25.0%
!! TTTTCATTAGATATGTGTTC+TGG + Chr2:34288043-34288062 MsG0280008580.01.T01:intron 25.0%
!!! TAAGTGCTTATTTTGTAACA+TGG + Chr2:34290075-34290094 MsG0280008580.01.T01:intron 25.0%
!!! TTTGTGTTTCCTTTTGATTA+GGG + Chr2:34288777-34288796 MsG0280008580.01.T01:intron 25.0%
!!! TTTTGTGTTTCCTTTTGATT+AGG + Chr2:34288776-34288795 MsG0280008580.01.T01:intron 25.0%
!!! TTTTTTCCTTTGATTATTCG+TGG + Chr2:34288640-34288659 MsG0280008580.01.T01:intron 25.0%
!!! TTTTTTCCTTTGATTTTTGG+TGG + Chr2:34287884-34287903 MsG0280008580.01.T01:intron 25.0%
AAAAAGTGAAGGAGAAATTG+AGG - Chr2:34287691-34287710 None:intergenic 30.0%
AAAAGTGAAGGAGAAATTGA+GGG - Chr2:34287690-34287709 None:intergenic 30.0%
AAACTACCACGAATAATCAA+AGG - Chr2:34288649-34288668 None:intergenic 30.0%
AATCATTGTTGAAGTCACAA+TGG - Chr2:34290739-34290758 None:intergenic 30.0%
GAAATTGAGGGTTAAATTGA+AGG - Chr2:34287678-34287697 None:intergenic 30.0%
GCAAATTTCAGTTAAAGCAT+GGG + Chr2:34289899-34289918 MsG0280008580.01.T01:CDS 30.0%
TAAAAGCTACGCAGATTATA+AGG + Chr2:34289576-34289595 MsG0280008580.01.T01:CDS 30.0%
TCTTACGTTATGGTTTGATT+CGG + Chr2:34289290-34289309 MsG0280008580.01.T01:intron 30.0%
TGTATGTATGGAAATATACC+TGG - Chr2:34288525-34288544 None:intergenic 30.0%
TTCTTCTTGTTAGCCAATAA+AGG - Chr2:34288698-34288717 None:intergenic 30.0%
TTTATCAAATGGACGAGTAT+CGG + Chr2:34290664-34290683 MsG0280008580.01.T01:CDS 30.0%
! AAAGCTGCTTACAATCTTTT+TGG + Chr2:34289799-34289818 MsG0280008580.01.T01:CDS 30.0%
! GATGATGATGATGATGATTA+GGG + Chr2:34287840-34287859 MsG0280008580.01.T01:intron 30.0%
! GTTTGAAACGAATCAAAAGT+AGG - Chr2:34289466-34289485 None:intergenic 30.0%
! TGATGATGATGATGATGATT+AGG + Chr2:34287839-34287858 MsG0280008580.01.T01:intron 30.0%
! TTGTAACACTGCATTTGTTT+TGG + Chr2:34288136-34288155 MsG0280008580.01.T01:intron 30.0%
!! ACATTTGGTTTGGATGTATT+CGG - Chr2:34289373-34289392 None:intergenic 30.0%
!! AGAATTTCAGGATTTTGAAG+AGG - Chr2:34287985-34288004 None:intergenic 30.0%
!! CCCTAAGCTATTTTATCAAA+TGG + Chr2:34290653-34290672 MsG0280008580.01.T01:CDS 30.0%
!! GAATTTCAGGATTTTGAAGA+GGG - Chr2:34287984-34288003 None:intergenic 30.0%
!! TGTAACACTGCATTTGTTTT+GGG + Chr2:34288137-34288156 MsG0280008580.01.T01:intron 30.0%
!! TTGTGTTTCCTTTTGATTAG+GGG + Chr2:34288778-34288797 MsG0280008580.01.T01:intron 30.0%
!!! AAACCAAATGTGGTTTTTTG+CGG + Chr2:34289382-34289401 MsG0280008580.01.T01:intron 30.0%
!!! CTTTTTTATATCTTGCGGTT+CGG - Chr2:34288835-34288854 None:intergenic 30.0%
!!! TCATTTCTTTTGGATTGGTT+CGG + Chr2:34289429-34289448 MsG0280008580.01.T01:intron 30.0%
!!! TTGTATATAGTTTTGGTAGG+TGG + Chr2:34288168-34288187 MsG0280008580.01.T01:intron 30.0%
AAACCGCAAAAAACCACATT+TGG - Chr2:34289388-34289407 None:intergenic 35.0%
AAATCGATGAAGAAGAAAGG+GGG - Chr2:34287643-34287662 None:intergenic 35.0%
AACAACACCGACAATATTCT+AGG - Chr2:34290768-34290787 None:intergenic 35.0%
ACTTCTTCGTGAAAAAGTGA+AGG - Chr2:34287702-34287721 None:intergenic 35.0%
AGAATCCTATGGTGAAATTG+TGG + Chr2:34290455-34290474 MsG0280008580.01.T01:CDS 35.0%
AGGAAATCGATGAAGAAGAA+AGG - Chr2:34287646-34287665 None:intergenic 35.0%
AGTCTCTTTCAATGTTTCCA+AGG + Chr2:34290422-34290441 MsG0280008580.01.T01:CDS 35.0%
ATGAAAACAAGGGTTTCAGT+TGG - Chr2:34289508-34289527 None:intergenic 35.0%
ATTAGATATGTGTTCTGGTG+TGG + Chr2:34288048-34288067 MsG0280008580.01.T01:intron 35.0%
CAACTACCACCAAAAATCAA+AGG - Chr2:34287893-34287912 None:intergenic 35.0%
CCATGTGGTTGAGTTAAAAA+AGG + Chr2:34289345-34289364 MsG0280008580.01.T01:intron 35.0%
CGCAAATTTCAGTTAAAGCA+TGG + Chr2:34289898-34289917 MsG0280008580.01.T01:CDS 35.0%
GAAATCGATGAAGAAGAAAG+GGG - Chr2:34287644-34287663 None:intergenic 35.0%
GAATACATCCAAACCAAATG+TGG + Chr2:34289372-34289391 MsG0280008580.01.T01:intron 35.0%
GAATCATGTTCCTCCTTTAT+TGG + Chr2:34288682-34288701 MsG0280008580.01.T01:CDS 35.0%
GAATCCTATGGTGAAATTGT+GGG + Chr2:34290456-34290475 MsG0280008580.01.T01:CDS 35.0%
GAATGTTTCAGTTACAAGCT+TGG + Chr2:34290353-34290372 MsG0280008580.01.T01:CDS 35.0%
GCAAAAAACCACATTTGGTT+TGG - Chr2:34289383-34289402 None:intergenic 35.0%
GGAAATCGATGAAGAAGAAA+GGG - Chr2:34287645-34287664 None:intergenic 35.0%
TACGTAGTTGTGAACATTGT+TGG - Chr2:34289085-34289104 None:intergenic 35.0%
TGGAAACATTGAAAGAGACT+GGG - Chr2:34290422-34290441 None:intergenic 35.0%
TTCATTGTAAACCATCGCTT+GGG + Chr2:34289643-34289662 MsG0280008580.01.T01:CDS 35.0%
TTCTTGTTAGCCAATAAAGG+AGG - Chr2:34288695-34288714 None:intergenic 35.0%
TTGCAAGAAAGCGAAACTAT+TGG + Chr2:34289867-34289886 MsG0280008580.01.T01:CDS 35.0%
TTGGAAACATTGAAAGAGAC+TGG - Chr2:34290423-34290442 None:intergenic 35.0%
TTTAACTCAACCACATGGTT+TGG - Chr2:34289343-34289362 None:intergenic 35.0%
! GTATTGGTGTTCTTACGTTA+TGG + Chr2:34289280-34289299 MsG0280008580.01.T01:intron 35.0%
! TAATCTGCGTAGCTTTTAAC+AGG - Chr2:34289574-34289593 None:intergenic 35.0%
! TACAAGCTTGGATGATTTTC+TGG + Chr2:34290365-34290384 MsG0280008580.01.T01:CDS 35.0%
!! TTGTCGGTGTTGTTAATCAA+TGG + Chr2:34290774-34290793 MsG0280008580.01.T01:three_prime_UTR 35.0%
!!! AATGTGGTTTTTTGCGGTTT+CGG + Chr2:34289388-34289407 MsG0280008580.01.T01:intron 35.0%
!!! CCTTTTTTAACTCAACCACA+TGG - Chr2:34289348-34289367 None:intergenic 35.0%
!!! GCTAGTTTTGGTGAAATTTG+GGG + Chr2:34288223-34288242 MsG0280008580.01.T01:intron 35.0%
!!! GGCTAGTTTTGGTGAAATTT+GGG + Chr2:34288222-34288241 MsG0280008580.01.T01:intron 35.0%
!!! GTAACACTGCATTTGTTTTG+GGG + Chr2:34288138-34288157 MsG0280008580.01.T01:intron 35.0%
!!! GTGTGTTTTGCTGTTTTCAT+TGG + Chr2:34288098-34288117 MsG0280008580.01.T01:intron 35.0%
!!! TGGCTAGTTTTGGTGAAATT+TGG + Chr2:34288221-34288240 MsG0280008580.01.T01:intron 35.0%
AAGAAAGGGGGGAAAAACTA+GGG - Chr2:34287631-34287650 None:intergenic 40.0%
AATCGATGAAGAAGAAAGGG+GGG - Chr2:34287642-34287661 None:intergenic 40.0%
ACACATAGCGACTTTCCAAT+AGG - Chr2:34290616-34290635 None:intergenic 40.0%
ACATAGCGACTTTCCAATAG+GGG - Chr2:34290614-34290633 None:intergenic 40.0%
AGCAGCTTTCATAACCTCTA+CGG - Chr2:34289788-34289807 None:intergenic 40.0%
CAAACCAAACCAATGCAGTT+TGG + Chr2:34288911-34288930 MsG0280008580.01.T01:intron 40.0%
CAAACCAATGCAGTTTGGAT+TGG + Chr2:34288916-34288935 MsG0280008580.01.T01:intron 40.0%
CACATAGCGACTTTCCAATA+GGG - Chr2:34290615-34290634 None:intergenic 40.0%
CCGCAAGATATAAAAAAGCG+CGG + Chr2:34288837-34288856 MsG0280008580.01.T01:intron 40.0%
CGTAGAGCAAATTAAGAACG+TGG + Chr2:34289101-34289120 MsG0280008580.01.T01:intron 40.0%
CTGGATCCTGATATAAACGA+CGG - Chr2:34288506-34288525 None:intergenic 40.0%
GACGATGATGATTAGGGTTA+AGG + Chr2:34288602-34288621 MsG0280008580.01.T01:intron 40.0%
GATAACAAATGGACACAGTG+AGG - Chr2:34290306-34290325 None:intergenic 40.0%
GCATAATGACCTTCCAATGA+CGG - Chr2:34289825-34289844 None:intergenic 40.0%
GTGCAAGACGATGATGATTA+GGG + Chr2:34288596-34288615 MsG0280008580.01.T01:intron 40.0%
GTTCATTGTAAACCATCGCT+TGG + Chr2:34289642-34289661 MsG0280008580.01.T01:CDS 40.0%
TACATAGAGAGACAACCAAG+AGG - Chr2:34288736-34288755 None:intergenic 40.0%
TGAAAACAGCAAAACACACG+CGG - Chr2:34288097-34288116 None:intergenic 40.0%
TGATCCAATCCAAACTGCAT+TGG - Chr2:34288923-34288942 None:intergenic 40.0%
TGGTTTACAATGAACGAGAC+TGG - Chr2:34289637-34289656 None:intergenic 40.0%
TGTGCAAGACGATGATGATT+AGG + Chr2:34288595-34288614 MsG0280008580.01.T01:intron 40.0%
TTCACGAAGAAGTCTCCAAA+TGG + Chr2:34287709-34287728 MsG0280008580.01.T01:exon 40.0%
TTCTTGCAATCCCCATAATC+TGG - Chr2:34289856-34289875 None:intergenic 40.0%
TTGGTAGGTGGAATCTATGT+GGG + Chr2:34288180-34288199 MsG0280008580.01.T01:intron 40.0%
TTTCAATGTTTCCAAGGACG+TGG + Chr2:34290428-34290447 MsG0280008580.01.T01:CDS 40.0%
TTTGGTAGGTGGAATCTATG+TGG + Chr2:34288179-34288198 MsG0280008580.01.T01:intron 40.0%
! AGAAGAGGGTTTGTTCCATT+TGG - Chr2:34287727-34287746 None:intergenic 40.0%
! CGCAAGATATAAAAAAGCGC+GGG + Chr2:34288838-34288857 MsG0280008580.01.T01:intron 40.0%
! CTGATAGGGAGTTTGTGTTT+TGG + Chr2:34288247-34288266 MsG0280008580.01.T01:intron 40.0%
! CTTAACCTTTTCTTCCTGGA+CGG - Chr2:34289949-34289968 None:intergenic 40.0%
! GACACTTAACCTTTTCTTCC+TGG - Chr2:34289953-34289972 None:intergenic 40.0%
! GATATAAAAAAGCGCGGGTT+TGG + Chr2:34288843-34288862 MsG0280008580.01.T01:intron 40.0%
! GTGAAATTTGGGGTCTGATA+GGG + Chr2:34288233-34288252 MsG0280008580.01.T01:intron 40.0%
! GTTTAGATTTGCTTCCCCAA+CGG - Chr2:34290178-34290197 None:intergenic 40.0%
!! ACAATCTTTTTGGCCGTCAT+TGG + Chr2:34289809-34289828 MsG0280008580.01.T01:CDS 40.0%
!! ACATGGTTTGGTTTGGTTTG+CGG - Chr2:34289331-34289350 None:intergenic 40.0%
!! ACGTTATGGTTTGATTCGGT+TGG + Chr2:34289294-34289313 MsG0280008580.01.T01:intron 40.0%
!! ATAGGGAGTTTGTGTTTTGG+TGG + Chr2:34288250-34288269 MsG0280008580.01.T01:intron 40.0%
!! CAAACTGCATTGGTTTGGTT+TGG - Chr2:34288913-34288932 None:intergenic 40.0%
!! CAATCCAAACTGCATTGGTT+TGG - Chr2:34288918-34288937 None:intergenic 40.0%
!! GGTGTTGTTAATCAATGGAG+TGG + Chr2:34290779-34290798 MsG0280008580.01.T01:three_prime_UTR 40.0%
!! TGCATTGGTTTGGTTTGGTT+CGG - Chr2:34288908-34288927 None:intergenic 40.0%
!!! AGAGTGTTTGGTTCTTTTGC+TGG - Chr2:34288472-34288491 None:intergenic 40.0%
!!! CCGCGCTTTTTTATATCTTG+CGG - Chr2:34288840-34288859 None:intergenic 40.0%
!!! CGTGGTAGTTTTTAGCTTGA+AGG + Chr2:34288658-34288677 MsG0280008580.01.T01:intron 40.0%
!!! CTTTTGATTAGGGGTGTTTG+CGG + Chr2:34288787-34288806 MsG0280008580.01.T01:intron 40.0%
!!! GTGTTTGTGTTGGCTAGTTT+TGG + Chr2:34288211-34288230 MsG0280008580.01.T01:intron 40.0%
!!! GTTTTTTGCGGTTTCGGTTT+TGG + Chr2:34289394-34289413 MsG0280008580.01.T01:intron 40.0%
!!! TAGGGAGTTTGTGTTTTGGT+GGG + Chr2:34288251-34288270 MsG0280008580.01.T01:intron 40.0%
AAAAGATGCTAGCTCCGTTG+GGG + Chr2:34290161-34290180 MsG0280008580.01.T01:intron 45.0%
ACAATGCTATTGGCCCCTAT+TGG + Chr2:34290598-34290617 MsG0280008580.01.T01:CDS 45.0%
ACCAAGAGGACTTACATGGA+CGG - Chr2:34288722-34288741 None:intergenic 45.0%
ACGCCTTAGCAACGTAATGT+TGG + Chr2:34289699-34289718 MsG0280008580.01.T01:CDS 45.0%
ACTTATGTAGGAAGGGTCAC+CGG + Chr2:34289539-34289558 MsG0280008580.01.T01:intron 45.0%
AGCTTTCATAACCTCTACGG+AGG - Chr2:34289785-34289804 None:intergenic 45.0%
AGGAACAAACAACCGGATAC+TGG - Chr2:34289765-34289784 None:intergenic 45.0%
ATGCGTTCACGACCAGATTA+TGG + Chr2:34289841-34289860 MsG0280008580.01.T01:CDS 45.0%
CGCAAACACCCCTAATCAAA+AGG - Chr2:34288789-34288808 None:intergenic 45.0%
CGCCTTAGCAACGTAATGTT+GGG + Chr2:34289700-34289719 MsG0280008580.01.T01:CDS 45.0%
CGTCGTTTATATCAGGATCC+AGG + Chr2:34288504-34288523 MsG0280008580.01.T01:intron 45.0%
GAAAAGATGCTAGCTCCGTT+GGG + Chr2:34290160-34290179 MsG0280008580.01.T01:intron 45.0%
GAAGAAAGGGGGGAAAAACT+AGG - Chr2:34287632-34287651 None:intergenic 45.0%
GACAACCAAGAGGACTTACA+TGG - Chr2:34288726-34288745 None:intergenic 45.0%
GACCCAACATTACGTTGCTA+AGG - Chr2:34289705-34289724 None:intergenic 45.0%
GACGTGGATACAGAATCCTA+TGG + Chr2:34290444-34290463 MsG0280008580.01.T01:CDS 45.0%
GCAAACCAAACCAAACCATG+TGG + Chr2:34289330-34289349 MsG0280008580.01.T01:intron 45.0%
TCTACGGAGGAACAAACAAC+CGG - Chr2:34289772-34289791 None:intergenic 45.0%
TGAAAAGATGCTAGCTCCGT+TGG + Chr2:34290159-34290178 MsG0280008580.01.T01:intron 45.0%
TGCAAGAAAACACAGAGTCG+CGG - Chr2:34288315-34288334 None:intergenic 45.0%
TGCGTTCACGACCAGATTAT+GGG + Chr2:34289842-34289861 MsG0280008580.01.T01:CDS 45.0%
TGGTAGGTGGAATCTATGTG+GGG + Chr2:34288181-34288200 MsG0280008580.01.T01:intron 45.0%
TGTTAATCAATGGAGTGGCC+TGG + Chr2:34290784-34290803 MsG0280008580.01.T01:three_prime_UTR 45.0%
! AAAAAAGCGCGGGTTTGGTT+TGG + Chr2:34288848-34288867 MsG0280008580.01.T01:intron 45.0%
! CTCTACTTGCTAGCAACACT+CGG + Chr2:34290481-34290500 MsG0280008580.01.T01:CDS 45.0%
! CTTAGTACTAGTCTCATGCC+AGG - Chr2:34290805-34290824 None:intergenic 45.0%
! GCAGTTTGGATTGGATCAGT+TGG + Chr2:34288925-34288944 MsG0280008580.01.T01:intron 45.0%
! GGTGAAATTTGGGGTCTGAT+AGG + Chr2:34288232-34288251 MsG0280008580.01.T01:intron 45.0%
! GGTTTCAGTTGGTGTTCCAA+AGG - Chr2:34289497-34289516 None:intergenic 45.0%
! GTTCTTTTGCTGGACAACCT+AGG - Chr2:34288462-34288481 None:intergenic 45.0%
! GTTGTTTCACAATGCACGAC+CGG - Chr2:34290558-34290577 None:intergenic 45.0%
!! CTCAACCACATGGTTTGGTT+TGG - Chr2:34289338-34289357 None:intergenic 45.0%
!! GGGTTTGGTTTGGTTTGGTT+CGG + Chr2:34288858-34288877 MsG0280008580.01.T01:intron 45.0%
!! TCTTTTTGGCCGTCATTGGA+AGG + Chr2:34289813-34289832 MsG0280008580.01.T01:CDS 45.0%
AAGGGGGGAAAAACTAGGGT+TGG - Chr2:34287627-34287646 None:intergenic 50.0%
AGCGCCCACAATTTCACCAT+AGG - Chr2:34290463-34290482 None:intergenic 50.0%
AGGGGGGAAAAACTAGGGTT+GGG - Chr2:34287626-34287645 None:intergenic 50.0%
CACTCGCCGTCGTTTATATC+AGG + Chr2:34288497-34288516 MsG0280008580.01.T01:intron 50.0%
GCGTTCACGACCAGATTATG+GGG + Chr2:34289843-34289862 MsG0280008580.01.T01:CDS 50.0%
GGATTCTGTATCCACGTCCT+TGG - Chr2:34290442-34290461 None:intergenic 50.0%
GGTAGGTGGAATCTATGTGG+GGG + Chr2:34288182-34288201 MsG0280008580.01.T01:intron 50.0%
GGTTGTTTGTTCCTCCGTAG+AGG + Chr2:34289771-34289790 MsG0280008580.01.T01:CDS 50.0%
GTAGGTGGAATCTATGTGGG+GGG + Chr2:34288183-34288202 MsG0280008580.01.T01:intron 50.0%
TGCTGGGATTGAAGACTGCT+GGG + Chr2:34290692-34290711 MsG0280008580.01.T01:CDS 50.0%
TGGAGACGACTGTGAGAAGA+GGG - Chr2:34287741-34287760 None:intergenic 50.0%
! AGGGCGTTACCTTCTCTTCT+TGG - Chr2:34287965-34287984 None:intergenic 50.0%
! GGTGTGGTTTTCCTTTCTGC+TGG + Chr2:34288064-34288083 MsG0280008580.01.T01:intron 50.0%
! TACTTGCTAGCAACACTCGG+TGG + Chr2:34290484-34290503 MsG0280008580.01.T01:CDS 50.0%
! TTGGTGGGTGGTGTCTTTGT+AGG + Chr2:34288266-34288285 MsG0280008580.01.T01:intron 50.0%
! TTTTAACAGGCGCACGACTC+CGG - Chr2:34289561-34289580 None:intergenic 50.0%
!! ACGAGTATCGGATGACTGCT+GGG + Chr2:34290676-34290695 MsG0280008580.01.T01:CDS 50.0%
!! GCGAGTGTGAGAAGAGTGTT+TGG - Chr2:34288484-34288503 None:intergenic 50.0%
!!! GGAGTTTGTGTTTTGGTGGG+TGG + Chr2:34288254-34288273 MsG0280008580.01.T01:intron 50.0%
AACCGGATACTGGCAGTGAC+CGG - Chr2:34289755-34289774 None:intergenic 55.0%
CTCAGCCGTCCAGGAAGAAA+AGG + Chr2:34289941-34289960 MsG0280008580.01.T01:CDS 55.0%
CTCAGCGCTCCAAGAAGAGA+AGG + Chr2:34287953-34287972 MsG0280008580.01.T01:intron 55.0%
CTCTCTCTCTCTCTCTGCCT+AGG + Chr2:34288442-34288461 MsG0280008580.01.T01:intron 55.0%
CTCTTCTCACAGTCGTCTCC+AGG + Chr2:34287740-34287759 MsG0280008580.01.T01:CDS 55.0%
CTGCTGGGATTGAAGACTGC+TGG + Chr2:34290691-34290710 MsG0280008580.01.T01:CDS 55.0%
CTGGAGACGACTGTGAGAAG+AGG - Chr2:34287742-34287761 None:intergenic 55.0%
GCCGTCCATGTAAGTCCTCT+TGG + Chr2:34288718-34288737 MsG0280008580.01.T01:intron 55.0%
GGGGGGAAAAACTAGGGTTG+GGG - Chr2:34287625-34287644 None:intergenic 55.0%
GTTTCACAATGCACGACCGG+TGG - Chr2:34290555-34290574 None:intergenic 55.0%
TTTCTTCCTGGACGGCTGAG+CGG - Chr2:34289941-34289960 None:intergenic 55.0%
!! AGCGCGGGTTTGGTTTGGTT+TGG + Chr2:34288853-34288872 MsG0280008580.01.T01:intron 55.0%
!! GACGAGTATCGGATGACTGC+TGG + Chr2:34290675-34290694 MsG0280008580.01.T01:CDS 55.0%
!! GATTAGGGGTGTTTGCGGTG+CGG + Chr2:34288792-34288811 MsG0280008580.01.T01:intron 55.0%
!! TCTCTTCTTGGAGCGCTGAG+CGG - Chr2:34287953-34287972 None:intergenic 55.0%
ACCCGGTCACTGCCAGTATC+CGG + Chr2:34289750-34289769 MsG0280008580.01.T01:CDS 60.0%
ACCGGATACTGGCAGTGACC+GGG - Chr2:34289754-34289773 None:intergenic 60.0%
ATCCGATCACTTCCCGCCAC+CGG + Chr2:34290536-34290555 MsG0280008580.01.T01:CDS 60.0%
ATTCTACCGCTCAGCCGTCC+AGG + Chr2:34289932-34289951 MsG0280008580.01.T01:CDS 60.0%
GCGGCTGTCATCCAGCAGAA+AGG - Chr2:34288078-34288097 None:intergenic 60.0%
GGCGGGAAGTGATCGGATCT+TGG - Chr2:34290534-34290553 None:intergenic 60.0%
GGGGGCGGTTGTGTTTGTGT+TGG + Chr2:34288201-34288220 MsG0280008580.01.T01:intron 60.0%
GGTGGAATCTATGTGGGGGG+CGG + Chr2:34288186-34288205 MsG0280008580.01.T01:intron 60.0%
TACTGGCAGTGACCGGGTCT+TGG - Chr2:34289748-34289767 None:intergenic 60.0%
TCACAATGCACGACCGGTGG+CGG - Chr2:34290552-34290571 None:intergenic 60.0%
AGTGCGCTCGACCCAAGCGA+TGG - Chr2:34289657-34289676 None:intergenic 65.0%
CACAATGCACGACCGGTGGC+GGG - Chr2:34290551-34290570 None:intergenic 65.0%
!! GACCGGTGGCGGGAAGTGAT+CGG - Chr2:34290541-34290560 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 34287598 34290898 34287598 ID=MsG0280008580.01;Name=MsG0280008580.01
Chr2 mRNA 34287598 34290898 34287598 ID=MsG0280008580.01.T01;Parent=MsG0280008580.01;Name=MsG0280008580.01.T01;_AED=0.42;_eAED=0.42;_QI=130|0.66|1|1|1|1|4|179|296
Chr2 exon 34287598 34287761 34287598 ID=MsG0280008580.01.T01:exon:15201;Parent=MsG0280008580.01.T01
Chr2 exon 34288666 34288726 34288666 ID=MsG0280008580.01.T01:exon:15202;Parent=MsG0280008580.01.T01
Chr2 exon 34289549 34289962 34289549 ID=MsG0280008580.01.T01:exon:15203;Parent=MsG0280008580.01.T01
Chr2 exon 34290338 34290898 34290338 ID=MsG0280008580.01.T01:exon:15204;Parent=MsG0280008580.01.T01
Chr2 five_prime_UTR 34287598 34287727 34287598 ID=MsG0280008580.01.T01:five_prime_utr;Parent=MsG0280008580.01.T01
Chr2 CDS 34287728 34287761 34287728 ID=MsG0280008580.01.T01:cds;Parent=MsG0280008580.01.T01
Chr2 CDS 34288666 34288726 34288666 ID=MsG0280008580.01.T01:cds;Parent=MsG0280008580.01.T01
Chr2 CDS 34289549 34289962 34289549 ID=MsG0280008580.01.T01:cds;Parent=MsG0280008580.01.T01
Chr2 CDS 34290338 34290719 34290338 ID=MsG0280008580.01.T01:cds;Parent=MsG0280008580.01.T01
Chr2 three_prime_UTR 34290720 34290898 34290720 ID=MsG0280008580.01.T01:three_prime_utr;Parent=MsG0280008580.01.T01
Gene Sequence

>MsG0280008580.01.T01

ATGGAACAAACCCTCTTCTCACAGTCGTCTCCAGTTTTTAGCTTGAAGGTGAATCATGTTCCTCCTTTATTGGCTAACAAGAAGAAGCCGTCCATGAAGGGTCACCGGAGTCGTGCGCCTGTTAAAAGCTACGCAGATTATAAGGTTAAGCTTAATGATTATGTAGAAAATAATGTTGGACCAGTCTCGTTCATTGTAAACCATCGCTTGGGTCGAGCGCACTACGAACGTATTTTGTATGATCTACGCCTTAGCAACGTAATGTTGGGTCGACGTGACAAAATTAAAAATCCAAGACCCGGTCACTGCCAGTATCCGGTTGTTTGTTCCTCCGTAGAGGTTATGAAAGCTGCTTACAATCTTTTTGGCCGTCATTGGAAGGTCATTATGCGTTCACGACCAGATTATGGGGATTGCAAGAAAGCGAAACTATTGGCTGAAATGCGCAAATTTCAGTTAAAGCATGGGATTGATATTCATTCTACCGCTCAGCCGTCCAGGAAGAAAAGCACAAAGACTCGAGTGAATGTTTCAGTTACAAGCTTGGATGATTTTCTGGATTCGCTTCAGCAGAACATTAATAATGCTGACCCAGTCTCTTTCAATGTTTCCAAGGACGTGGATACAGAATCCTATGGTGAAATTGTGGGCGCTCTACTTGCTAGCAACACTCGGTGGAATCGATCTGACAAAATCAAACATCCAAGATCCGATCACTTCCCGCCACCGGTCGTGCATTGTGAAACAACAGAAAAGATGAAAAATGTTTACAATGCTATTGGCCCCTATTGGAAAGTCGCTATGTGTGTGTGTGAACATGACAACCCTAAGCTATTTTATCAAATGGACGAGTATCGGATGACTGCTGGGATTGAAGACTGCTGGGATTGA
Protein sequence

>MsG0280008580.01.T01

MEQTLFSQSSPVFSLKVNHVPPLLANKKKPSMKGHRSRAPVKSYADYKVKLNDYVENNVGPVSFIVNHRLGRAHYERILYDLRLSNVMLGRRDKIKNPRPGHCQYPVVCSSVEVMKAAYNLFGRHWKVIMRSRPDYGDCKKAKLLAEMRKFQLKHGIDIHSTAQPSRKKSTKTRVNVSVTSLDDFLDSLQQNINNADPVSFNVSKDVDTESYGEIVGALLASNTRWNRSDKIKHPRSDHFPPPVVHCETTEKMKNVYNAIGPYWKVAMCVCEHDNPKLFYQMDEYRMTAGIEDCWD*