AlfalfaGEDB | MsG0280009516.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009516.01

KEGG: K02935
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF00542

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009516.01.T01	XP_013464191.1	95.833	216	9	0	1	216	1	216	3.29E-139	398

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009516.01.T01	B3EER1	41.538	130	65	3	86	215	6	124	1.91E-17	78.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009516.01.T01	A0A072V9A8	95.833	216	9	0	1	216	1	216	1.57e-139	398

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0280009516.01	MsG0180005007.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009516.01.T01	MTR_2g064300	95.833	216	9	0	1	216	1	216	3.99e-143	398
MsG0280009516.01.T01	MTR_7g053520	83.871	217	34	1	1	216	1	217	3.50e-120	340
MsG0280009516.01.T01	MTR_2g031760	40.606	165	93	3	53	216	30	190	4.83e-33	118
MsG0280009516.01.T01	MTR_2g031960	47.857	140	66	3	79	216	21	155	8.05e-31	111
MsG0280009516.01.T01	MTR_8g076780	44.737	152	74	2	74	216	45	195	1.16e-29	109

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009516.01.T01	AT1G70190	53.182	220	87	3	1	216	1	208	1.25e-65	201
MsG0280009516.01.T01	AT1G70190	53.182	220	87	3	1	216	1	208	1.25e-65	201
MsG0280009516.01.T01	AT4G36420	44.211	190	91	4	30	216	2	179	1.98e-34	121
MsG0280009516.01.T01	AT3G06040	41.611	149	80	1	68	216	45	186	5.86e-28	104
MsG0280009516.01.T01	AT3G06040	41.611	149	80	1	68	216	45	186	5.86e-28	104
MsG0280009516.01.T01	AT3G06040	41.611	149	80	1	68	216	45	186	5.86e-28	104
MsG0280009516.01.T01	AT4G37660	38.798	183	89	3	34	216	8	167	4.66e-25	96.7
MsG0280009516.01.T01	AT2G03130	57.500	80	34	0	137	216	12	91	1.14e-15	70.5

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Find 54 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTGTTTCAAAGGAAGA+AGG	0.492660	2:-53461649	MsG0280009516.01.T01:CDS
AAAGTCATCAAGGAGGTGCG+GGG	0.667732	2:-53461736	MsG0280009516.01.T01:CDS
AATGTGGGATATATGAAAGC+AGG	0.529942	2:-53461877	MsG0280009516.01.T01:CDS
ACACCGTCTATTATAAAGAA+AGG	0.512263	2:-53461667	MsG0280009516.01.T01:CDS
ACACCTTTCTTTATAATAGA+CGG	0.526861	2:+53461664	None:intergenic
ACTTGGTGCTAATGTTGTTT+TGG	0.411925	2:-53461599	MsG0280009516.01.T01:CDS
AGAGGAGGAGGAAGACGTAG+AGG	0.548567	2:-53462088	MsG0280009516.01.T01:CDS
AGCCACGACTGCAGCCCAAG+AGG	0.642383	2:-53461824	MsG0280009516.01.T01:CDS
AGCCTGAAAACTTTCTCTGA+TGG	0.532734	2:+53461988	None:intergenic
AGTTGCAAGAAAAGAGGAGG+AGG	0.555547	2:-53462100	MsG0280009516.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACACCGTCTATTATAAAGAA+AGG	-	Chr2:53462149-53462168	MsG0280009516.01.T01:CDS	30.0%
ATAAAAGAAATGCCCAATGT+GGG	-	Chr2:53461924-53461943	MsG0280009516.01.T01:CDS	30.0%
CAAAATTAAAGTCATCAAGG+AGG	-	Chr2:53462073-53462092	MsG0280009516.01.T01:CDS	30.0%
AATGTGGGATATATGAAAGC+AGG	-	Chr2:53461939-53461958	MsG0280009516.01.T01:CDS	35.0%
ATGTGGGATATATGAAAGCA+GGG	-	Chr2:53461940-53461959	MsG0280009516.01.T01:CDS	35.0%
CCTAATACGCTTTCATAAAC+TGG	+	Chr2:53461651-53461670	None:intergenic	35.0%
GATAAAAGAAATGCCCAATG+TGG	-	Chr2:53461923-53461942	MsG0280009516.01.T01:CDS	35.0%
GCTTTCATATATCCCACATT+GGG	+	Chr2:53461939-53461958	None:intergenic	35.0%
TGAAAACTTTCTCTGATGGA+GGG	+	Chr2:53461827-53461846	None:intergenic	35.0%
TGCTTTCATATATCCCACAT+TGG	+	Chr2:53461940-53461959	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	53461594	53462244	53461594	ID=MsG0280009516.01;Name=MsG0280009516.01
Chr2	mRNA	53461594	53462244	53461594	ID=MsG0280009516.01.T01;Parent=MsG0280009516.01;Name=MsG0280009516.01.T01;_AED=0.42;_eAED=0.42;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|216
Chr2	exon	53461594	53462244	53461594	ID=MsG0280009516.01.T01:exon:38176;Parent=MsG0280009516.01.T01
Chr2	CDS	53461594	53462244	53461594	ID=MsG0280009516.01.T01:cds;Parent=MsG0280009516.01.T01

Gene Sequence

>MsG0280009516.01.T01

ATGAGCTTGCTGTTGAGAATAAGGCATCGTTTACACAATGGTTTGTATAGACAACCAGTTTATGAAAGCGTATTAGGGCTTAATGCTAGGAAATTAAATGTATTTTCGAGAAACTTTGGTCAAGTTGCAAGAAAAGAGGAGGAGGAAGACGTAGAGGAAGTGGAAATTGATCAAAGAAGTCTCCCAGCTGATTTTGATCCTGCAACATTTGATCCCAATAACAATCGTGGCCCTCCATCAGAGAAAGTTTTCAGGCTTGTTGATGAAGTGGCGTCTCTAACGCTAGCTGAAGGTGCGGAATTGGGTCTCATTTTGATGAAGAAAATGGGGATAAAAGAAATGCCCAATGTGGGATATATGAAAGCAGGGTCTGCAAATTTGGCCGGAATGGCAACAAAAGCCACGACTGCAGCCCAAGAGGAGGTAAAACCAGAAAAAACTGTATTTGAATTGAAACTGTTGTCGTATGAAGCAGCTTCCAAAATTAAAGTCATCAAGGAGGTGCGGGGCTTTACCGATTTAGGTTTGAAGGAGGCAAAGGATATTGTCGAAAAAACACCGTCTATTATAAAGAAAGGTGTTTCAAAGGAAGAAGGAGAACAGATAATAGAGAAAATGAAAGCACTTGGTGCTAATGTTGTTTTGGAGTGA

Protein sequence

>MsG0280009516.01.T01

MSLLLRIRHRLHNGLYRQPVYESVLGLNARKLNVFSRNFGQVARKEEEEDVEEVEIDQRSLPADFDPATFDPNNNRGPPSEKVFRLVDEVASLTLAEGAELGLILMKKMGIKEMPNVGYMKAGSANLAGMATKATTAAQEEVKPEKTVFELKLLSYEAASKIKVIKEVRGFTDLGLKEAKDIVEKTPSIIKKGVSKEEGEQIIEKMKALGANVVLE*