Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009516.01.T01 | XP_013464191.1 | 95.833 | 216 | 9 | 0 | 1 | 216 | 1 | 216 | 3.29E-139 | 398 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009516.01.T01 | B3EER1 | 41.538 | 130 | 65 | 3 | 86 | 215 | 6 | 124 | 1.91E-17 | 78.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009516.01.T01 | A0A072V9A8 | 95.833 | 216 | 9 | 0 | 1 | 216 | 1 | 216 | 1.57e-139 | 398 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0280009516.01 | MsG0180005007.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009516.01.T01 | MTR_2g064300 | 95.833 | 216 | 9 | 0 | 1 | 216 | 1 | 216 | 3.99e-143 | 398 |
| MsG0280009516.01.T01 | MTR_7g053520 | 83.871 | 217 | 34 | 1 | 1 | 216 | 1 | 217 | 3.50e-120 | 340 |
| MsG0280009516.01.T01 | MTR_2g031760 | 40.606 | 165 | 93 | 3 | 53 | 216 | 30 | 190 | 4.83e-33 | 118 |
| MsG0280009516.01.T01 | MTR_2g031960 | 47.857 | 140 | 66 | 3 | 79 | 216 | 21 | 155 | 8.05e-31 | 111 |
| MsG0280009516.01.T01 | MTR_8g076780 | 44.737 | 152 | 74 | 2 | 74 | 216 | 45 | 195 | 1.16e-29 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009516.01.T01 | AT1G70190 | 53.182 | 220 | 87 | 3 | 1 | 216 | 1 | 208 | 1.25e-65 | 201 |
| MsG0280009516.01.T01 | AT1G70190 | 53.182 | 220 | 87 | 3 | 1 | 216 | 1 | 208 | 1.25e-65 | 201 |
| MsG0280009516.01.T01 | AT4G36420 | 44.211 | 190 | 91 | 4 | 30 | 216 | 2 | 179 | 1.98e-34 | 121 |
| MsG0280009516.01.T01 | AT3G06040 | 41.611 | 149 | 80 | 1 | 68 | 216 | 45 | 186 | 5.86e-28 | 104 |
| MsG0280009516.01.T01 | AT3G06040 | 41.611 | 149 | 80 | 1 | 68 | 216 | 45 | 186 | 5.86e-28 | 104 |
| MsG0280009516.01.T01 | AT3G06040 | 41.611 | 149 | 80 | 1 | 68 | 216 | 45 | 186 | 5.86e-28 | 104 |
| MsG0280009516.01.T01 | AT4G37660 | 38.798 | 183 | 89 | 3 | 34 | 216 | 8 | 167 | 4.66e-25 | 96.7 |
| MsG0280009516.01.T01 | AT2G03130 | 57.500 | 80 | 34 | 0 | 137 | 216 | 12 | 91 | 1.14e-15 | 70.5 |
Find 54 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCTTGATGACTTTAATTT+TGG | 0.115213 | 2:+53461744 | None:intergenic |
| CCAGTTTATGAAAGCGTATT+AGG | 0.115413 | 2:-53462168 | MsG0280009516.01.T01:CDS |
| TGGAGGGCCACGATTGTTAT+TGG | 0.240332 | 2:+53462008 | None:intergenic |
| GAAAGCAGGGTCTGCAAATT+TGG | 0.254350 | 2:-53461863 | MsG0280009516.01.T01:CDS |
| GTGCGGGGCTTTACCGATTT+AGG | 0.299345 | 2:-53461721 | MsG0280009516.01.T01:CDS |
| CCTAATACGCTTTCATAAAC+TGG | 0.317127 | 2:+53462168 | None:intergenic |
| TGAGCTTGCTGTTGAGAATA+AGG | 0.322760 | 2:-53462221 | MsG0280009516.01.T01:CDS |
| GCTAGCTGAAGGTGCGGAAT+TGG | 0.332644 | 2:-53461941 | MsG0280009516.01.T01:CDS |
| CAGTTTATGAAAGCGTATTA+GGG | 0.345172 | 2:-53462167 | MsG0280009516.01.T01:CDS |
| TGCCTCCTTCAAACCTAAAT+CGG | 0.380631 | 2:+53461708 | None:intergenic |
| GGAGGGCCACGATTGTTATT+GGG | 0.390326 | 2:+53462009 | None:intergenic |
| GGAGGAAGACGTAGAGGAAG+TGG | 0.397120 | 2:-53462082 | MsG0280009516.01.T01:CDS |
| ACTTGGTGCTAATGTTGTTT+TGG | 0.411925 | 2:-53461599 | MsG0280009516.01.T01:CDS |
| CTAGCTGAAGGTGCGGAATT+GGG | 0.422415 | 2:-53461940 | MsG0280009516.01.T01:CDS |
| TTATTGGGATCAAATGTTGC+AGG | 0.430449 | 2:+53462024 | None:intergenic |
| CTTTACCGATTTAGGTTTGA+AGG | 0.453564 | 2:-53461713 | MsG0280009516.01.T01:CDS |
| TAAAGTCATCAAGGAGGTGC+GGG | 0.464501 | 2:-53461737 | MsG0280009516.01.T01:CDS |
| GTCTGCAAATTTGGCCGGAA+TGG | 0.467477 | 2:-53461854 | MsG0280009516.01.T01:CDS |
| GCTTTCATATATCCCACATT+GGG | 0.475885 | 2:+53461880 | None:intergenic |
| CTCCTCTTGGGCTGCAGTCG+TGG | 0.476435 | 2:+53461822 | None:intergenic |
| AAAGGTGTTTCAAAGGAAGA+AGG | 0.492660 | 2:-53461649 | MsG0280009516.01.T01:CDS |
| GTTGCAGGATCAAAATCAGC+TGG | 0.495579 | 2:+53462039 | None:intergenic |
| TATAAAGAAAGGTGTTTCAA+AGG | 0.503102 | 2:-53461656 | MsG0280009516.01.T01:CDS |
| ACACCGTCTATTATAAAGAA+AGG | 0.512263 | 2:-53461667 | MsG0280009516.01.T01:CDS |
| TGCTTTCATATATCCCACAT+TGG | 0.515923 | 2:+53461879 | None:intergenic |
| TGGTCAAGTTGCAAGAAAAG+AGG | 0.520139 | 2:-53462106 | MsG0280009516.01.T01:CDS |
| GCGTATTAGGGCTTAATGCT+AGG | 0.525999 | 2:-53462155 | MsG0280009516.01.T01:CDS |
| ACACCTTTCTTTATAATAGA+CGG | 0.526861 | 2:+53461664 | None:intergenic |
| TTCCAAAATTAAAGTCATCA+AGG | 0.526956 | 2:-53461746 | MsG0280009516.01.T01:CDS |
| AATGTGGGATATATGAAAGC+AGG | 0.529942 | 2:-53461877 | MsG0280009516.01.T01:CDS |
| AGCCTGAAAACTTTCTCTGA+TGG | 0.532734 | 2:+53461988 | None:intergenic |
| CTGAAAACTTTCTCTGATGG+AGG | 0.535890 | 2:+53461991 | None:intergenic |
| GCAGGGTCTGCAAATTTGGC+CGG | 0.545113 | 2:-53461859 | MsG0280009516.01.T01:CDS |
| TTTCAGGCTTGTTGATGAAG+TGG | 0.545631 | 2:-53461974 | MsG0280009516.01.T01:CDS |
| AGAGGAGGAGGAAGACGTAG+AGG | 0.548567 | 2:-53462088 | MsG0280009516.01.T01:CDS |
| AGTTGCAAGAAAAGAGGAGG+AGG | 0.555547 | 2:-53462100 | MsG0280009516.01.T01:CDS |
| TTTAGGTTTGAAGGAGGCAA+AGG | 0.562989 | 2:-53461704 | MsG0280009516.01.T01:CDS |
| TGAAAACTTTCTCTGATGGA+GGG | 0.607842 | 2:+53461992 | None:intergenic |
| GCGTCTCTAACGCTAGCTGA+AGG | 0.607910 | 2:-53461952 | MsG0280009516.01.T01:CDS |
| GATAAAAGAAATGCCCAATG+TGG | 0.608771 | 2:-53461893 | MsG0280009516.01.T01:CDS |
| TTAAAGTCATCAAGGAGGTG+CGG | 0.612909 | 2:-53461738 | MsG0280009516.01.T01:CDS |
| ATAGAGAAAATGAAAGCACT+TGG | 0.623385 | 2:-53461616 | MsG0280009516.01.T01:CDS |
| ATGTGGGATATATGAAAGCA+GGG | 0.623877 | 2:-53461876 | MsG0280009516.01.T01:CDS |
| ATAAAAGAAATGCCCAATGT+GGG | 0.625462 | 2:-53461892 | MsG0280009516.01.T01:CDS |
| TACCGATTTAGGTTTGAAGG+AGG | 0.626116 | 2:-53461710 | MsG0280009516.01.T01:CDS |
| CACGACTGCAGCCCAAGAGG+AGG | 0.628136 | 2:-53461821 | MsG0280009516.01.T01:CDS |
| TTGCAGGATCAAAATCAGCT+GGG | 0.640796 | 2:+53462040 | None:intergenic |
| AGCCACGACTGCAGCCCAAG+AGG | 0.642383 | 2:-53461824 | MsG0280009516.01.T01:CDS |
| ATAAGGCATCGTTTACACAA+TGG | 0.647228 | 2:-53462204 | MsG0280009516.01.T01:CDS |
| TCAAGTTGCAAGAAAAGAGG+AGG | 0.661992 | 2:-53462103 | MsG0280009516.01.T01:CDS |
| AAAGTCATCAAGGAGGTGCG+GGG | 0.667732 | 2:-53461736 | MsG0280009516.01.T01:CDS |
| TTTGATCCCAATAACAATCG+TGG | 0.681002 | 2:-53462015 | MsG0280009516.01.T01:CDS |
| TCTAACGCTAGCTGAAGGTG+CGG | 0.697298 | 2:-53461947 | MsG0280009516.01.T01:CDS |
| CAAAATTAAAGTCATCAAGG+AGG | 0.704497 | 2:-53461743 | MsG0280009516.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTCAAATACAGTTTTTTC+TGG | + | Chr2:53462026-53462045 | None:intergenic | 20.0% |
| ! | ACACCTTTCTTTATAATAGA+CGG | + | Chr2:53462155-53462174 | None:intergenic | 25.0% |
| ! | TTCCAAAATTAAAGTCATCA+AGG | - | Chr2:53462070-53462089 | MsG0280009516.01.T01:CDS | 25.0% |
| !!! | AATGTATTTTCGAGAAACTT+TGG | - | Chr2:53461690-53461709 | MsG0280009516.01.T01:CDS | 25.0% |
| !!! | CTCATTTTGATGAAGAAAAT+GGG | - | Chr2:53461900-53461919 | MsG0280009516.01.T01:CDS | 25.0% |
| !!! | TATAAAGAAAGGTGTTTCAA+AGG | - | Chr2:53462160-53462179 | MsG0280009516.01.T01:CDS | 25.0% |
| !!! | TCATTTTGATGAAGAAAATG+GGG | - | Chr2:53461901-53461920 | MsG0280009516.01.T01:CDS | 25.0% |
| !!! | TCTCATTTTGATGAAGAAAA+TGG | - | Chr2:53461899-53461918 | MsG0280009516.01.T01:CDS | 25.0% |
| ACACCGTCTATTATAAAGAA+AGG | - | Chr2:53462149-53462168 | MsG0280009516.01.T01:CDS | 30.0% | |
| ATAAAAGAAATGCCCAATGT+GGG | - | Chr2:53461924-53461943 | MsG0280009516.01.T01:CDS | 30.0% | |
| CAAAATTAAAGTCATCAAGG+AGG | - | Chr2:53462073-53462092 | MsG0280009516.01.T01:CDS | 30.0% | |
| ! | CAGTTTATGAAAGCGTATTA+GGG | - | Chr2:53461649-53461668 | MsG0280009516.01.T01:CDS | 30.0% |
| !! | ATAGAGAAAATGAAAGCACT+TGG | - | Chr2:53462200-53462219 | MsG0280009516.01.T01:CDS | 30.0% |
| !! | CTCCTTGATGACTTTAATTT+TGG | + | Chr2:53462075-53462094 | None:intergenic | 30.0% |
| AATGTGGGATATATGAAAGC+AGG | - | Chr2:53461939-53461958 | MsG0280009516.01.T01:CDS | 35.0% | |
| ATGTGGGATATATGAAAGCA+GGG | - | Chr2:53461940-53461959 | MsG0280009516.01.T01:CDS | 35.0% | |
| CCTAATACGCTTTCATAAAC+TGG | + | Chr2:53461651-53461670 | None:intergenic | 35.0% | |
| GATAAAAGAAATGCCCAATG+TGG | - | Chr2:53461923-53461942 | MsG0280009516.01.T01:CDS | 35.0% | |
| GCTTTCATATATCCCACATT+GGG | + | Chr2:53461939-53461958 | None:intergenic | 35.0% | |
| TGAAAACTTTCTCTGATGGA+GGG | + | Chr2:53461827-53461846 | None:intergenic | 35.0% | |
| TGCTTTCATATATCCCACAT+TGG | + | Chr2:53461940-53461959 | None:intergenic | 35.0% | |
| TTATTGGGATCAAATGTTGC+AGG | + | Chr2:53461795-53461814 | None:intergenic | 35.0% | |
| TTTGATCCCAATAACAATCG+TGG | - | Chr2:53461801-53461820 | MsG0280009516.01.T01:CDS | 35.0% | |
| ! | CCAGTTTATGAAAGCGTATT+AGG | - | Chr2:53461648-53461667 | MsG0280009516.01.T01:CDS | 35.0% |
| !! | AAAGGTGTTTCAAAGGAAGA+AGG | - | Chr2:53462167-53462186 | MsG0280009516.01.T01:CDS | 35.0% |
| !! | ATAAGGCATCGTTTACACAA+TGG | - | Chr2:53461612-53461631 | MsG0280009516.01.T01:CDS | 35.0% |
| !! | CTTTACCGATTTAGGTTTGA+AGG | - | Chr2:53462103-53462122 | MsG0280009516.01.T01:CDS | 35.0% |
| !!! | ACTTGGTGCTAATGTTGTTT+TGG | - | Chr2:53462217-53462236 | MsG0280009516.01.T01:CDS | 35.0% |
| AGCCTGAAAACTTTCTCTGA+TGG | + | Chr2:53461831-53461850 | None:intergenic | 40.0% | |
| CTGAAAACTTTCTCTGATGG+AGG | + | Chr2:53461828-53461847 | None:intergenic | 40.0% | |
| TGCCTCCTTCAAACCTAAAT+CGG | + | Chr2:53462111-53462130 | None:intergenic | 40.0% | |
| TTAAAGTCATCAAGGAGGTG+CGG | - | Chr2:53462078-53462097 | MsG0280009516.01.T01:CDS | 40.0% | |
| TTGCAGGATCAAAATCAGCT+GGG | + | Chr2:53461779-53461798 | None:intergenic | 40.0% | |
| ! | CTCCATCAGAGAAAGTTTTC+AGG | - | Chr2:53461826-53461845 | MsG0280009516.01.T01:CDS | 40.0% |
| ! | TCAAGTTGCAAGAAAAGAGG+AGG | - | Chr2:53461713-53461732 | MsG0280009516.01.T01:CDS | 40.0% |
| ! | TGGTCAAGTTGCAAGAAAAG+AGG | - | Chr2:53461710-53461729 | MsG0280009516.01.T01:CDS | 40.0% |
| ! | TTTAGGTTTGAAGGAGGCAA+AGG | - | Chr2:53462112-53462131 | MsG0280009516.01.T01:CDS | 40.0% |
| !! | TACCGATTTAGGTTTGAAGG+AGG | - | Chr2:53462106-53462125 | MsG0280009516.01.T01:CDS | 40.0% |
| !! | TGAGCTTGCTGTTGAGAATA+AGG | - | Chr2:53461595-53461614 | MsG0280009516.01.T01:CDS | 40.0% |
| !! | TTTCAGGCTTGTTGATGAAG+TGG | - | Chr2:53461842-53461861 | MsG0280009516.01.T01:CDS | 40.0% |
| !!! | TTCTGGTTTTACCTCCTCTT+GGG | + | Chr2:53462009-53462028 | None:intergenic | 40.0% |
| !!! | TTTCTGGTTTTACCTCCTCT+TGG | + | Chr2:53462010-53462029 | None:intergenic | 40.0% |
| AGTTGCAAGAAAAGAGGAGG+AGG | - | Chr2:53461716-53461735 | MsG0280009516.01.T01:CDS | 45.0% | |
| GAAAGCAGGGTCTGCAAATT+TGG | - | Chr2:53461953-53461972 | MsG0280009516.01.T01:CDS | 45.0% | |
| GCGTATTAGGGCTTAATGCT+AGG | - | Chr2:53461661-53461680 | MsG0280009516.01.T01:CDS | 45.0% | |
| GTTGCAGGATCAAAATCAGC+TGG | + | Chr2:53461780-53461799 | None:intergenic | 45.0% | |
| ! | TAAAGTCATCAAGGAGGTGC+GGG | - | Chr2:53462079-53462098 | MsG0280009516.01.T01:CDS | 45.0% |
| GTCTGCAAATTTGGCCGGAA+TGG | - | Chr2:53461962-53461981 | MsG0280009516.01.T01:CDS | 50.0% | |
| TCTAACGCTAGCTGAAGGTG+CGG | - | Chr2:53461869-53461888 | MsG0280009516.01.T01:CDS | 50.0% | |
| TGGAGGGCCACGATTGTTAT+TGG | + | Chr2:53461811-53461830 | None:intergenic | 50.0% | |
| ! | AAAGTCATCAAGGAGGTGCG+GGG | - | Chr2:53462080-53462099 | MsG0280009516.01.T01:CDS | 50.0% |
| ! | CGTGGCTTTTGTTGCCATTC+CGG | + | Chr2:53461979-53461998 | None:intergenic | 50.0% |
| ! | CTAGCTGAAGGTGCGGAATT+GGG | - | Chr2:53461876-53461895 | MsG0280009516.01.T01:CDS | 50.0% |
| ! | GGAGGGCCACGATTGTTATT+GGG | + | Chr2:53461810-53461829 | None:intergenic | 50.0% |
| AGAGGAGGAGGAAGACGTAG+AGG | - | Chr2:53461728-53461747 | MsG0280009516.01.T01:CDS | 55.0% | |
| GCAGGGTCTGCAAATTTGGC+CGG | - | Chr2:53461957-53461976 | MsG0280009516.01.T01:CDS | 55.0% | |
| GCGTCTCTAACGCTAGCTGA+AGG | - | Chr2:53461864-53461883 | MsG0280009516.01.T01:CDS | 55.0% | |
| GGAGGAAGACGTAGAGGAAG+TGG | - | Chr2:53461734-53461753 | MsG0280009516.01.T01:CDS | 55.0% | |
| ! | GCTAGCTGAAGGTGCGGAAT+TGG | - | Chr2:53461875-53461894 | MsG0280009516.01.T01:CDS | 55.0% |
| !! | GTGCGGGGCTTTACCGATTT+AGG | - | Chr2:53462095-53462114 | MsG0280009516.01.T01:CDS | 55.0% |
| AGCCACGACTGCAGCCCAAG+AGG | - | Chr2:53461992-53462011 | MsG0280009516.01.T01:CDS | 65.0% | |
| CACGACTGCAGCCCAAGAGG+AGG | - | Chr2:53461995-53462014 | MsG0280009516.01.T01:CDS | 65.0% | |
| CTCCTCTTGGGCTGCAGTCG+TGG | + | Chr2:53461997-53462016 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 53461594 | 53462244 | 53461594 | ID=MsG0280009516.01;Name=MsG0280009516.01 |
| Chr2 | mRNA | 53461594 | 53462244 | 53461594 | ID=MsG0280009516.01.T01;Parent=MsG0280009516.01;Name=MsG0280009516.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|216 |
| Chr2 | exon | 53461594 | 53462244 | 53461594 | ID=MsG0280009516.01.T01:exon:38176;Parent=MsG0280009516.01.T01 |
| Chr2 | CDS | 53461594 | 53462244 | 53461594 | ID=MsG0280009516.01.T01:cds;Parent=MsG0280009516.01.T01 |
| Gene Sequence |
| Protein sequence |
