Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009782.01.T01 | RHN74679.1 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 3.83E-64 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280009781.01 | MsG0280009782.01 | 0.905087 | 6.215699e-80 | 9.896953e-76 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTACCGGGTGTTCCGGTAC+TGG | 0.232775 | 2:+57869044 | MsG0280009782.01.T01:CDS |
| TGAAGTTCACCTATGGCTTT+TGG | 0.246944 | 2:+57868798 | MsG0280009782.01.T01:CDS |
| GGGCGAAGTTACCGGCAATT+TGG | 0.291864 | 2:+57869066 | MsG0280009782.01.T01:CDS |
| CGGTACTGGGGCGAAGTTAC+CGG | 0.344478 | 2:+57869058 | MsG0280009782.01.T01:CDS |
| ATTGGAAGAGATTGGGTTAC+CGG | 0.364909 | 2:+57869029 | MsG0280009782.01.T01:CDS |
| TTACCGGGTGTTCCGGTACT+GGG | 0.380858 | 2:+57869045 | MsG0280009782.01.T01:CDS |
| TGCCGGTAATTGGAAGAGAT+TGG | 0.382906 | 2:+57869021 | MsG0280009782.01.T01:CDS |
| TTGGGCCAAACGGTGGGTTT+CGG | 0.388071 | 2:+57868848 | MsG0280009782.01.T01:CDS |
| GATTGGGTTACCGGGTGTTC+CGG | 0.396051 | 2:+57869038 | MsG0280009782.01.T01:CDS |
| ACCCAATCTCTTCCAATTAC+CGG | 0.404661 | 2:-57869023 | None:intergenic |
| GACCCACGTGTCCAAATTGC+CGG | 0.410101 | 2:-57869077 | None:intergenic |
| GCCGGTAATTGGAAGAGATT+GGG | 0.420406 | 2:+57869022 | MsG0280009782.01.T01:CDS |
| CTTCACCGAAACCCACCGTT+TGG | 0.441137 | 2:-57868853 | None:intergenic |
| AAGAAGTGGTGACCGGCTAC+TGG | 0.454076 | 2:+57868939 | MsG0280009782.01.T01:CDS |
| AAACACCGTCAATGTTGGTT+GGG | 0.487542 | 2:+57868994 | MsG0280009782.01.T01:CDS |
| CCGGCTACTGGCTCGTAAAG+TGG | 0.489439 | 2:+57868951 | MsG0280009782.01.T01:CDS |
| TACCGTCTCCGTCGAAGAAG+TGG | 0.490800 | 2:+57868925 | MsG0280009782.01.T01:CDS |
| AAAGCCATAGGTGAACTTCA+TGG | 0.491487 | 2:-57868795 | None:intergenic |
| GGAGCCGTTTGTGAATTTAA+CGG | 0.496268 | 2:+57868893 | MsG0280009782.01.T01:CDS |
| TACTGGCTCGTAAAGTGGAT+TGG | 0.503226 | 2:+57868956 | MsG0280009782.01.T01:CDS |
| AAAGTGGATTGGCGCCGTTA+CGG | 0.506853 | 2:+57868967 | MsG0280009782.01.T01:CDS |
| CGCCCCAGTACCGGAACACC+CGG | 0.514755 | 2:-57869048 | None:intergenic |
| CGGTAACTTCGCCCCAGTAC+CGG | 0.516194 | 2:-57869057 | None:intergenic |
| CACGCCGTTAAATTCACAAA+CGG | 0.525415 | 2:-57868897 | None:intergenic |
| AAAATCCCAACCAACATTGA+CGG | 0.549872 | 2:-57868999 | None:intergenic |
| CCACTTTACGAGCCAGTAGC+CGG | 0.552479 | 2:-57868951 | None:intergenic |
| AACGGTGGGTTTCGGTGAAG+CGG | 0.555250 | 2:+57868856 | MsG0280009782.01.T01:CDS |
| GAGGTAAACACCGTCAATGT+TGG | 0.560388 | 2:+57868989 | MsG0280009782.01.T01:CDS |
| TAAACACCGTCAATGTTGGT+TGG | 0.561530 | 2:+57868993 | MsG0280009782.01.T01:CDS |
| ATGGCCATGAAGTTCACCTA+TGG | 0.568344 | 2:+57868791 | MsG0280009782.01.T01:CDS |
| AATTTGGACACGTGGGTCTG+AGG | 0.572286 | 2:+57869082 | MsG0280009782.01.T01:CDS |
| CTTCTTCGACGGAGACGGTA+TGG | 0.575538 | 2:-57868922 | None:intergenic |
| TTGGAAGAGATTGGGTTACC+GGG | 0.578033 | 2:+57869030 | MsG0280009782.01.T01:CDS |
| GACGGTGTTTACCTCCGTAA+CGG | 0.579350 | 2:-57868981 | None:intergenic |
| CACCACTTCTTCGACGGAGA+CGG | 0.597813 | 2:-57868927 | None:intergenic |
| TCCGTCGAAGAAGTGGTGAC+CGG | 0.598430 | 2:+57868932 | MsG0280009782.01.T01:CDS |
| GTGGATTGGCGCCGTTACGG+AGG | 0.602740 | 2:+57868970 | MsG0280009782.01.T01:CDS |
| TACCGGGTGTTCCGGTACTG+GGG | 0.613065 | 2:+57869046 | MsG0280009782.01.T01:CDS |
| GCCGGTCACCACTTCTTCGA+CGG | 0.627345 | 2:-57868933 | None:intergenic |
| TTACCGGCAATTTGGACACG+TGG | 0.639659 | 2:+57869074 | MsG0280009782.01.T01:CDS |
| CGTTTGTGAATTTAACGGCG+TGG | 0.669554 | 2:+57868898 | MsG0280009782.01.T01:CDS |
| TACCGGCAATTTGGACACGT+GGG | 0.676913 | 2:+57869075 | MsG0280009782.01.T01:CDS |
| GAAGCGGCAAGAGTAACTGA+CGG | 0.714014 | 2:+57868872 | MsG0280009782.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATCCCAACCAACATTGA+CGG | - | Chr2:57869002-57869021 | None:intergenic | 35.0% | |
| !! | ACCTATGGCTTTTGGAAAAA+CGG | + | Chr2:57868806-57868825 | MsG0280009782.01.T01:CDS | 35.0% |
| AAAGCCATAGGTGAACTTCA+TGG | - | Chr2:57868798-57868817 | None:intergenic | 40.0% | |
| ACCCAATCTCTTCCAATTAC+CGG | - | Chr2:57869026-57869045 | None:intergenic | 40.0% | |
| ATTGGAAGAGATTGGGTTAC+CGG | + | Chr2:57869029-57869048 | MsG0280009782.01.T01:CDS | 40.0% | |
| CACGCCGTTAAATTCACAAA+CGG | - | Chr2:57868900-57868919 | None:intergenic | 40.0% | |
| GGAGCCGTTTGTGAATTTAA+CGG | + | Chr2:57868893-57868912 | MsG0280009782.01.T01:CDS | 40.0% | |
| TAAACACCGTCAATGTTGGT+TGG | + | Chr2:57868993-57869012 | MsG0280009782.01.T01:CDS | 40.0% | |
| ! | AAACACCGTCAATGTTGGTT+GGG | + | Chr2:57868994-57869013 | MsG0280009782.01.T01:CDS | 40.0% |
| ! | TGAAGTTCACCTATGGCTTT+TGG | + | Chr2:57868798-57868817 | MsG0280009782.01.T01:CDS | 40.0% |
| !! | AATGTTGGTTGGGATTTTGC+CGG | + | Chr2:57869004-57869023 | MsG0280009782.01.T01:CDS | 40.0% |
| !! | CCTATGGCTTTTGGAAAAAC+GGG | + | Chr2:57868807-57868826 | MsG0280009782.01.T01:CDS | 40.0% |
| ATGGCCATGAAGTTCACCTA+TGG | + | Chr2:57868791-57868810 | MsG0280009782.01.T01:CDS | 45.0% | |
| CGTTTGTGAATTTAACGGCG+TGG | + | Chr2:57868898-57868917 | MsG0280009782.01.T01:CDS | 45.0% | |
| GAGGTAAACACCGTCAATGT+TGG | + | Chr2:57868989-57869008 | MsG0280009782.01.T01:CDS | 45.0% | |
| GCCGGTAATTGGAAGAGATT+GGG | + | Chr2:57869022-57869041 | MsG0280009782.01.T01:CDS | 45.0% | |
| TGCCGGTAATTGGAAGAGAT+TGG | + | Chr2:57869021-57869040 | MsG0280009782.01.T01:CDS | 45.0% | |
| TTGGAAGAGATTGGGTTACC+GGG | + | Chr2:57869030-57869049 | MsG0280009782.01.T01:CDS | 45.0% | |
| ! | GTTGGGATTTTGCCGGTAAT+TGG | + | Chr2:57869011-57869030 | MsG0280009782.01.T01:CDS | 45.0% |
| ! | TTTTTCTTGGGCCAAACGGT+GGG | + | Chr2:57868842-57868861 | MsG0280009782.01.T01:CDS | 45.0% |
| !! | CCCGTTTTTCCAAAAGCCAT+AGG | - | Chr2:57868810-57868829 | None:intergenic | 45.0% |
| !! | TACTGGCTCGTAAAGTGGAT+TGG | + | Chr2:57868956-57868975 | MsG0280009782.01.T01:CDS | 45.0% |
| GAAGCGGCAAGAGTAACTGA+CGG | + | Chr2:57868872-57868891 | MsG0280009782.01.T01:CDS | 50.0% | |
| GACGGTGTTTACCTCCGTAA+CGG | - | Chr2:57868984-57869003 | None:intergenic | 50.0% | |
| TACCGGCAATTTGGACACGT+GGG | + | Chr2:57869075-57869094 | MsG0280009782.01.T01:CDS | 50.0% | |
| TTACCGGCAATTTGGACACG+TGG | + | Chr2:57869074-57869093 | MsG0280009782.01.T01:CDS | 50.0% | |
| TTGGCCCAAGAAAAAGCCCT+GGG | - | Chr2:57868837-57868856 | None:intergenic | 50.0% | |
| TTTGGCCCAAGAAAAAGCCC+TGG | - | Chr2:57868838-57868857 | None:intergenic | 50.0% | |
| ! | CTTTTTCTTGGGCCAAACGG+TGG | + | Chr2:57868841-57868860 | MsG0280009782.01.T01:CDS | 50.0% |
| !! | AAAGTGGATTGGCGCCGTTA+CGG | + | Chr2:57868967-57868986 | MsG0280009782.01.T01:CDS | 50.0% |
| !! | GGGCTTTTTCTTGGGCCAAA+CGG | + | Chr2:57868838-57868857 | MsG0280009782.01.T01:CDS | 50.0% |
| !! | TGGCTTTTGGAAAAACGGGC+CGG | + | Chr2:57868811-57868830 | MsG0280009782.01.T01:CDS | 50.0% |
| AAGAAGTGGTGACCGGCTAC+TGG | + | Chr2:57868939-57868958 | MsG0280009782.01.T01:CDS | 55.0% | |
| CACCACTTCTTCGACGGAGA+CGG | - | Chr2:57868930-57868949 | None:intergenic | 55.0% | |
| CCACTTTACGAGCCAGTAGC+CGG | - | Chr2:57868954-57868973 | None:intergenic | 55.0% | |
| CTTCACCGAAACCCACCGTT+TGG | - | Chr2:57868856-57868875 | None:intergenic | 55.0% | |
| CTTCTTCGACGGAGACGGTA+TGG | - | Chr2:57868925-57868944 | None:intergenic | 55.0% | |
| GACCCACGTGTCCAAATTGC+CGG | - | Chr2:57869080-57869099 | None:intergenic | 55.0% | |
| GATTGGGTTACCGGGTGTTC+CGG | + | Chr2:57869038-57869057 | MsG0280009782.01.T01:CDS | 55.0% | |
| GGGCGAAGTTACCGGCAATT+TGG | + | Chr2:57869066-57869085 | MsG0280009782.01.T01:CDS | 55.0% | |
| TACCGTCTCCGTCGAAGAAG+TGG | + | Chr2:57868925-57868944 | MsG0280009782.01.T01:CDS | 55.0% | |
| TCCGTCGAAGAAGTGGTGAC+CGG | + | Chr2:57868932-57868951 | MsG0280009782.01.T01:CDS | 55.0% | |
| TTACCGGGTGTTCCGGTACT+GGG | + | Chr2:57869045-57869064 | MsG0280009782.01.T01:CDS | 55.0% | |
| ! | AACGGTGGGTTTCGGTGAAG+CGG | + | Chr2:57868856-57868875 | MsG0280009782.01.T01:CDS | 55.0% |
| ! | TTGGGCCAAACGGTGGGTTT+CGG | + | Chr2:57868848-57868867 | MsG0280009782.01.T01:CDS | 55.0% |
| CCCAAGAAAAAGCCCTGGGC+CGG | - | Chr2:57868833-57868852 | None:intergenic | 60.0% | |
| CGGTAACTTCGCCCCAGTAC+CGG | - | Chr2:57869060-57869079 | None:intergenic | 60.0% | |
| GCCGGTCACCACTTCTTCGA+CGG | - | Chr2:57868936-57868955 | None:intergenic | 60.0% | |
| GTTACCGGGTGTTCCGGTAC+TGG | + | Chr2:57869044-57869063 | MsG0280009782.01.T01:CDS | 60.0% | |
| TACCGGGTGTTCCGGTACTG+GGG | + | Chr2:57869046-57869065 | MsG0280009782.01.T01:CDS | 60.0% | |
| ! | CGGTACTGGGGCGAAGTTAC+CGG | + | Chr2:57869058-57869077 | MsG0280009782.01.T01:CDS | 60.0% |
| !! | CCGGCCCAGGGCTTTTTCTT+GGG | + | Chr2:57868830-57868849 | MsG0280009782.01.T01:CDS | 60.0% |
| !! | CCGGCTACTGGCTCGTAAAG+TGG | + | Chr2:57868951-57868970 | MsG0280009782.01.T01:CDS | 60.0% |
| TGGAAAAACGGGCCGGCCCA+GGG | + | Chr2:57868818-57868837 | MsG0280009782.01.T01:CDS | 65.0% | |
| TTGGAAAAACGGGCCGGCCC+AGG | + | Chr2:57868817-57868836 | MsG0280009782.01.T01:CDS | 65.0% | |
| !! | GCCGGCCCAGGGCTTTTTCT+TGG | + | Chr2:57868829-57868848 | MsG0280009782.01.T01:CDS | 65.0% |
| !! | GTGGATTGGCGCCGTTACGG+AGG | + | Chr2:57868970-57868989 | MsG0280009782.01.T01:CDS | 65.0% |
| ! | CGCCCCAGTACCGGAACACC+CGG | - | Chr2:57869051-57869070 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 57868791 | 57869102 | 57868791 | ID=MsG0280009782.01;Name=MsG0280009782.01 |
| Chr2 | mRNA | 57868791 | 57869102 | 57868791 | ID=MsG0280009782.01.T01;Parent=MsG0280009782.01;Name=MsG0280009782.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr2 | exon | 57868791 | 57869102 | 57868791 | ID=MsG0280009782.01.T01:exon:38376;Parent=MsG0280009782.01.T01 |
| Chr2 | CDS | 57868791 | 57869102 | 57868791 | ID=MsG0280009782.01.T01:cds;Parent=MsG0280009782.01.T01 |
| Gene Sequence |
| Protein sequence |
