Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010828.01.T01 | XP_013465238.1 | 94.853 | 136 | 7 | 0 | 1 | 136 | 1 | 136 | 2.79E-79 | 240 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010828.01.T01 | A0A392LXP5 | 95.588 | 136 | 6 | 0 | 1 | 136 | 1 | 136 | 1.33e-79 | 240 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003379.01 | MsG0280010828.01 | 0.816231 | 6.150749e-52 | 4.333776e-49 |
| MsG0280010828.01 | MsG0680031828.01 | 0.800706 | 1.270557e-48 | 5.956234e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010828.01.T01 | MTR_2g091215 | 94.853 | 136 | 7 | 0 | 1 | 136 | 1 | 136 | 3.37e-83 | 240 |
| MsG0280010828.01.T01 | MTR_2g091210 | 80.882 | 136 | 26 | 0 | 1 | 136 | 1 | 136 | 1.02e-78 | 229 |
| MsG0280010828.01.T01 | MTR_7g103200 | 54.839 | 124 | 56 | 0 | 9 | 132 | 14 | 137 | 1.76e-45 | 145 |
| MsG0280010828.01.T01 | MTR_7g111900 | 50.806 | 124 | 61 | 0 | 9 | 132 | 14 | 137 | 3.00e-41 | 135 |
| MsG0280010828.01.T01 | MTR_1g084200 | 49.194 | 124 | 63 | 0 | 9 | 132 | 14 | 137 | 1.10e-38 | 129 |
| MsG0280010828.01.T01 | MTR_2g019480 | 41.803 | 122 | 71 | 0 | 11 | 132 | 20 | 141 | 1.05e-35 | 120 |
| MsG0280010828.01.T01 | MTR_2g046150 | 41.667 | 132 | 77 | 0 | 3 | 134 | 11 | 142 | 1.21e-35 | 120 |
| MsG0280010828.01.T01 | MTR_4g122930 | 42.963 | 135 | 73 | 2 | 4 | 136 | 9 | 141 | 2.87e-27 | 99.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010828.01.T01 | AT4G24130 | 41.985 | 131 | 76 | 0 | 2 | 132 | 11 | 141 | 1.87e-37 | 125 |
| MsG0280010828.01.T01 | AT1G56580 | 50.407 | 123 | 58 | 2 | 12 | 132 | 17 | 138 | 2.10e-37 | 125 |
| MsG0280010828.01.T01 | AT5G46230 | 40.000 | 130 | 78 | 0 | 3 | 132 | 10 | 139 | 3.27e-37 | 124 |
| MsG0280010828.01.T01 | AT1G09310 | 49.587 | 121 | 60 | 1 | 12 | 132 | 17 | 136 | 8.44e-37 | 124 |
| MsG0280010828.01.T01 | AT1G30020 | 47.826 | 92 | 48 | 0 | 16 | 107 | 23 | 114 | 9.22e-30 | 105 |
| MsG0280010828.01.T01 | AT5G49600 | 37.857 | 140 | 77 | 3 | 2 | 132 | 5 | 143 | 7.62e-22 | 85.9 |
| MsG0280010828.01.T01 | AT5G19860 | 32.203 | 118 | 74 | 3 | 15 | 132 | 31 | 142 | 3.01e-11 | 58.5 |
Find 26 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGATTAAGAAACTTAA+AGG | 0.217317 | 2:-75532338 | MsG0280010828.01.T01:CDS |
| CTTCTGGTAGTTCAAATTCT+TGG | 0.283168 | 2:+75532513 | None:intergenic |
| GCAAGAGGGAGAAGCCCTTC+TGG | 0.340972 | 2:+75532497 | None:intergenic |
| ATTCATTTCAAAAGTCTTGC+TGG | 0.341570 | 2:-75532242 | MsG0280010828.01.T01:CDS |
| CCAGCAGCAAAAGCTTCAAC+AGG | 0.396874 | 2:+75532203 | None:intergenic |
| TCAACAGTAATCTCATTAAC+AGG | 0.398107 | 2:+75532284 | None:intergenic |
| ACTGTTGATGAACAACCAAC+TGG | 0.402135 | 2:-75532269 | MsG0280010828.01.T01:CDS |
| AGTGGAGCATAAATTCAATA+TGG | 0.405831 | 2:-75532411 | MsG0280010828.01.T01:CDS |
| CTTGCTGATATTATCGAAAT+CGG | 0.419122 | 2:-75532479 | MsG0280010828.01.T01:CDS |
| TGAAAGCAACTGGTTTCATC+TGG | 0.419545 | 2:-75532451 | MsG0280010828.01.T01:CDS |
| ATCGGATACGTGAAAGCAAC+TGG | 0.443417 | 2:-75532461 | MsG0280010828.01.T01:CDS |
| AGCTATGATACTGAGATAAC+AGG | 0.449906 | 2:-75532374 | MsG0280010828.01.T01:CDS |
| AAGCTAAGGAACTCATGTTA+TGG | 0.504679 | 2:-75532310 | MsG0280010828.01.T01:CDS |
| GAAGGAGCCATTGTGAAGAA+AGG | 0.522679 | 2:-75532572 | MsG0280010828.01.T01:CDS |
| GTCGTGTCCTTTCTTCACAA+TGG | 0.535377 | 2:+75532565 | None:intergenic |
| TTGAAATGAATCTTCCCAGT+TGG | 0.542965 | 2:+75532254 | None:intergenic |
| AGCTTCAACAGGGAAAGTCT+TGG | 0.561609 | 2:+75532214 | None:intergenic |
| ACTTAAAGGTGTGAAAGCTA+AGG | 0.587041 | 2:-75532324 | MsG0280010828.01.T01:CDS |
| CAAGAATTTGAACTACCAGA+AGG | 0.592182 | 2:-75532512 | MsG0280010828.01.T01:CDS |
| GATTGCTCAGAAGAAGAAAG+TGG | 0.598871 | 2:-75532429 | MsG0280010828.01.T01:CDS |
| AAGAATTTGAACTACCAGAA+GGG | 0.607094 | 2:-75532511 | MsG0280010828.01.T01:CDS |
| ATTTCGATAATATCAGCAAG+AGG | 0.628664 | 2:+75532482 | None:intergenic |
| CTGTTGATGAACAACCAACT+GGG | 0.634789 | 2:-75532268 | MsG0280010828.01.T01:CDS |
| TTTCGATAATATCAGCAAGA+GGG | 0.656350 | 2:+75532483 | None:intergenic |
| CAGCAGCAAAAGCTTCAACA+GGG | 0.665125 | 2:+75532204 | None:intergenic |
| ACAGTAATCTCATTAACAGG+AGG | 0.681626 | 2:+75532287 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAGATTAAGAAACTTAA+AGG | - | Chr2:75532442-75532461 | MsG0280010828.01.T01:CDS | 20.0% |
| AAGAATTTGAACTACCAGAA+GGG | - | Chr2:75532269-75532288 | MsG0280010828.01.T01:CDS | 30.0% | |
| AGTGGAGCATAAATTCAATA+TGG | - | Chr2:75532369-75532388 | MsG0280010828.01.T01:CDS | 30.0% | |
| ATTCATTTCAAAAGTCTTGC+TGG | - | Chr2:75532538-75532557 | MsG0280010828.01.T01:CDS | 30.0% | |
| ATTTCGATAATATCAGCAAG+AGG | + | Chr2:75532301-75532320 | None:intergenic | 30.0% | |
| CTTGCTGATATTATCGAAAT+CGG | - | Chr2:75532301-75532320 | MsG0280010828.01.T01:CDS | 30.0% | |
| TCAACAGTAATCTCATTAAC+AGG | + | Chr2:75532499-75532518 | None:intergenic | 30.0% | |
| TTTCGATAATATCAGCAAGA+GGG | + | Chr2:75532300-75532319 | None:intergenic | 30.0% | |
| AAGCTAAGGAACTCATGTTA+TGG | - | Chr2:75532470-75532489 | MsG0280010828.01.T01:CDS | 35.0% | |
| ACAGTAATCTCATTAACAGG+AGG | + | Chr2:75532496-75532515 | None:intergenic | 35.0% | |
| AGCTATGATACTGAGATAAC+AGG | - | Chr2:75532406-75532425 | MsG0280010828.01.T01:CDS | 35.0% | |
| CAAGAATTTGAACTACCAGA+AGG | - | Chr2:75532268-75532287 | MsG0280010828.01.T01:CDS | 35.0% | |
| CTTCTGGTAGTTCAAATTCT+TGG | + | Chr2:75532270-75532289 | None:intergenic | 35.0% | |
| TTGAAATGAATCTTCCCAGT+TGG | + | Chr2:75532529-75532548 | None:intergenic | 35.0% | |
| ! | ACTTAAAGGTGTGAAAGCTA+AGG | - | Chr2:75532456-75532475 | MsG0280010828.01.T01:CDS | 35.0% |
| GATTGCTCAGAAGAAGAAAG+TGG | - | Chr2:75532351-75532370 | MsG0280010828.01.T01:CDS | 40.0% | |
| ! | ACTGTTGATGAACAACCAAC+TGG | - | Chr2:75532511-75532530 | MsG0280010828.01.T01:CDS | 40.0% |
| ! | CTGTTGATGAACAACCAACT+GGG | - | Chr2:75532512-75532531 | MsG0280010828.01.T01:CDS | 40.0% |
| ! | TGAAAGCAACTGGTTTCATC+TGG | - | Chr2:75532329-75532348 | MsG0280010828.01.T01:CDS | 40.0% |
| AGCTTCAACAGGGAAAGTCT+TGG | + | Chr2:75532569-75532588 | None:intergenic | 45.0% | |
| ATCGGATACGTGAAAGCAAC+TGG | - | Chr2:75532319-75532338 | MsG0280010828.01.T01:CDS | 45.0% | |
| CAGCAGCAAAAGCTTCAACA+GGG | + | Chr2:75532579-75532598 | None:intergenic | 45.0% | |
| GAAGGAGCCATTGTGAAGAA+AGG | - | Chr2:75532208-75532227 | MsG0280010828.01.T01:CDS | 45.0% | |
| GTCGTGTCCTTTCTTCACAA+TGG | + | Chr2:75532218-75532237 | None:intergenic | 45.0% | |
| CCAGCAGCAAAAGCTTCAAC+AGG | + | Chr2:75532580-75532599 | None:intergenic | 50.0% | |
| ! | CCTGTTGAAGCTTTTGCTGC+TGG | - | Chr2:75532577-75532596 | MsG0280010828.01.T01:CDS | 50.0% |
| GCAAGAGGGAGAAGCCCTTC+TGG | + | Chr2:75532286-75532305 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 75532196 | 75532606 | 75532196 | ID=MsG0280010828.01;Name=MsG0280010828.01 |
| Chr2 | mRNA | 75532196 | 75532606 | 75532196 | ID=MsG0280010828.01.T01;Parent=MsG0280010828.01;Name=MsG0280010828.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|136 |
| Chr2 | exon | 75532196 | 75532606 | 75532196 | ID=MsG0280010828.01.T01:exon:11161;Parent=MsG0280010828.01.T01 |
| Chr2 | CDS | 75532196 | 75532606 | 75532196 | ID=MsG0280010828.01.T01:cds;Parent=MsG0280010828.01.T01 |
| Gene Sequence |
| Protein sequence |
