Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011047.01.T01 | RHN79491.1 | 99.454 | 183 | 1 | 0 | 1 | 183 | 1 | 183 | 1.31E-133 | 381 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011047.01.T01 | Q54G07 | 31.667 | 180 | 108 | 5 | 8 | 181 | 8 | 178 | 5.34E-23 | 94.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011047.01.T01 | A0A396JRU3 | 99.454 | 183 | 1 | 0 | 1 | 183 | 1 | 183 | 6.25e-134 | 381 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003690.01 | MsG0280011047.01 | 0.808730 | 2.682778e-50 | 1.545604e-47 |
| MsG0280011047.01 | MsG0480019972.01 | 0.804541 | 2.055845e-49 | 1.062783e-46 |
| MsG0280011047.01 | MsG0580024073.01 | 0.800795 | 1.218904e-48 | 5.726995e-46 |
| MsG0280011047.01 | MsG0580029767.01 | 0.853286 | 2.642211e-61 | 5.679798e-58 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011047.01.T01 | MTR_8g096810 | 98.361 | 183 | 3 | 0 | 1 | 183 | 1 | 183 | 5.87e-136 | 377 |
| MsG0280011047.01.T01 | MTR_1g058050 | 98.907 | 183 | 2 | 0 | 1 | 183 | 77 | 259 | 9.94e-136 | 380 |
| MsG0280011047.01.T01 | MTR_2g098170 | 98.361 | 183 | 3 | 0 | 1 | 183 | 1 | 183 | 2.55e-135 | 375 |
| MsG0280011047.01.T01 | MTR_2g017985 | 97.268 | 183 | 5 | 0 | 1 | 183 | 1 | 183 | 7.32e-135 | 374 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011047.01.T01 | AT5G13070 | 72.928 | 181 | 47 | 1 | 1 | 179 | 1 | 181 | 1.53e-102 | 293 |
Find 41 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGTCTGCTTTGGCTTCTA+TGG | 0.285179 | 2:+78859848 | MsG0280011047.01.T01:CDS |
| GGTCGATGCAGATTTGTTAT+AGG | 0.304123 | 2:+78859709 | MsG0280011047.01.T01:CDS |
| TGCTAAGAGTAGAGATGTTA+TGG | 0.314934 | 2:+78859917 | MsG0280011047.01.T01:CDS |
| GTCCATCCATTCGGATTATC+AGG | 0.325224 | 2:-78859789 | None:intergenic |
| TTGTTATAGGAATATTAGTA+TGG | 0.335683 | 2:+78859722 | MsG0280011047.01.T01:CDS |
| GATTAAACCATTGTCTGCTT+TGG | 0.373627 | 2:+78859839 | MsG0280011047.01.T01:CDS |
| ACAAGCCTTGATTCATCTTC+CGG | 0.402248 | 2:+78859573 | MsG0280011047.01.T01:CDS |
| CAAGCCTTGATTCATCTTCC+GGG | 0.414079 | 2:+78859574 | MsG0280011047.01.T01:CDS |
| AACCGTTCGTTGTCCGTGGC+TGG | 0.420484 | 2:+78859620 | MsG0280011047.01.T01:CDS |
| TGGCTGGTACGAAGGATTAT+CGG | 0.421617 | 2:+78859636 | MsG0280011047.01.T01:CDS |
| GTCAGCAGGAGACGAGGATT+CGG | 0.428182 | 2:+78859817 | MsG0280011047.01.T01:CDS |
| TTGAGCAGAGGTGTGCTGAT+AGG | 0.440113 | 2:+78859880 | MsG0280011047.01.T01:CDS |
| ACGTGTCGCGTATAGCTTCC+CGG | 0.475968 | 2:-78859592 | None:intergenic |
| TATGGAGAAGTTTATTGAAG+TGG | 0.479069 | 2:+78859740 | MsG0280011047.01.T01:CDS |
| GTTGTCCGTGGCTGGTACGA+AGG | 0.479907 | 2:+78859628 | MsG0280011047.01.T01:CDS |
| AACATGTTTATAACCATCCA+TGG | 0.481341 | 2:+78859460 | MsG0280011047.01.T01:CDS |
| AGGTTACTCGTTCCCATGGA+TGG | 0.512718 | 2:-78859473 | None:intergenic |
| TGGATGGACTGTTTGTCAGC+AGG | 0.536254 | 2:+78859803 | MsG0280011047.01.T01:CDS |
| GCCGAGGTTACTCGTTCCCA+TGG | 0.538501 | 2:-78859477 | None:intergenic |
| GTCTGCTTTGGCTTCTATGG+CGG | 0.542260 | 2:+78859851 | MsG0280011047.01.T01:CDS |
| TGACAAACAGTCCATCCATT+CGG | 0.545003 | 2:-78859798 | None:intergenic |
| TTGAAGTGGAAGAGAAGACT+AGG | 0.557364 | 2:+78859754 | MsG0280011047.01.T01:CDS |
| AACGAGTAACCTCGGCATCA+TGG | 0.564625 | 2:+78859484 | MsG0280011047.01.T01:CDS |
| ATCCTGATAATCCGAATGGA+TGG | 0.575753 | 2:+78859787 | MsG0280011047.01.T01:CDS |
| TACCAGCCACGGACAACGAA+CGG | 0.582782 | 2:-78859622 | None:intergenic |
| GCTTCCCGGAAGATGAATCA+AGG | 0.585346 | 2:-78859578 | None:intergenic |
| CTATAACCGTTCGTTGTCCG+TGG | 0.586902 | 2:+78859616 | MsG0280011047.01.T01:CDS |
| CTGTTTGTCAGCAGGAGACG+AGG | 0.587525 | 2:+78859811 | MsG0280011047.01.T01:CDS |
| GAGGATTTGCAAATATCTTG+AGG | 0.598037 | 2:+78859941 | MsG0280011047.01.T01:CDS |
| CCTCATCCTGATAATCCGAA+TGG | 0.599257 | 2:+78859783 | MsG0280011047.01.T01:CDS |
| CCATTCGGATTATCAGGATG+AGG | 0.600517 | 2:-78859783 | None:intergenic |
| AGAGTAGAGATGTTATGGAG+AGG | 0.605987 | 2:+78859922 | MsG0280011047.01.T01:CDS |
| TCCATGGGAACGAGTAACCT+CGG | 0.631582 | 2:+78859476 | MsG0280011047.01.T01:CDS |
| ATAATCCTTCGTACCAGCCA+CGG | 0.637876 | 2:-78859633 | None:intergenic |
| ATAGAAGCCAAAGCAGACAA+TGG | 0.655161 | 2:-78859846 | None:intergenic |
| ACATGTTTATAACCATCCAT+GGG | 0.656615 | 2:+78859461 | MsG0280011047.01.T01:CDS |
| TGGCGGAGAAAGTTGAGCAG+AGG | 0.668340 | 2:+78859868 | MsG0280011047.01.T01:CDS |
| TTGTGTTGAATCAACTGTCG+TGG | 0.677952 | 2:+78859674 | MsG0280011047.01.T01:CDS |
| GTATTGCAATCTAGAATGTG+TGG | 0.699008 | 2:-78859543 | None:intergenic |
| TTATATCAGAAACAATCACA+AGG | 0.723613 | 2:-78859982 | None:intergenic |
| CTGTCGTGGATGCAAAATCG+CGG | 0.724194 | 2:+78859688 | MsG0280011047.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTTATAGGAATATTAGTA+TGG | + | Chr2:78859722-78859741 | MsG0280011047.01.T01:CDS | 20.0% |
| AACATGTTTATAACCATCCA+TGG | + | Chr2:78859460-78859479 | MsG0280011047.01.T01:CDS | 30.0% | |
| ACATGTTTATAACCATCCAT+GGG | + | Chr2:78859461-78859480 | MsG0280011047.01.T01:CDS | 30.0% | |
| TATGGAGAAGTTTATTGAAG+TGG | + | Chr2:78859740-78859759 | MsG0280011047.01.T01:CDS | 30.0% | |
| !! | TGGTAAAACACGTTTGTTTT+CGG | - | Chr2:78859526-78859545 | None:intergenic | 30.0% |
| GAGGATTTGCAAATATCTTG+AGG | + | Chr2:78859941-78859960 | MsG0280011047.01.T01:CDS | 35.0% | |
| GATTAAACCATTGTCTGCTT+TGG | + | Chr2:78859839-78859858 | MsG0280011047.01.T01:CDS | 35.0% | |
| TGCTAAGAGTAGAGATGTTA+TGG | + | Chr2:78859917-78859936 | MsG0280011047.01.T01:CDS | 35.0% | |
| ! | GTATTGCAATCTAGAATGTG+TGG | - | Chr2:78859546-78859565 | None:intergenic | 35.0% |
| ACAAGCCTTGATTCATCTTC+CGG | + | Chr2:78859573-78859592 | MsG0280011047.01.T01:CDS | 40.0% | |
| AGAGTAGAGATGTTATGGAG+AGG | + | Chr2:78859922-78859941 | MsG0280011047.01.T01:CDS | 40.0% | |
| ATAGAAGCCAAAGCAGACAA+TGG | - | Chr2:78859849-78859868 | None:intergenic | 40.0% | |
| ATCCTGATAATCCGAATGGA+TGG | + | Chr2:78859787-78859806 | MsG0280011047.01.T01:CDS | 40.0% | |
| TGACAAACAGTCCATCCATT+CGG | - | Chr2:78859801-78859820 | None:intergenic | 40.0% | |
| TTGAAGTGGAAGAGAAGACT+AGG | + | Chr2:78859754-78859773 | MsG0280011047.01.T01:CDS | 40.0% | |
| TTGTGTTGAATCAACTGTCG+TGG | + | Chr2:78859674-78859693 | MsG0280011047.01.T01:CDS | 40.0% | |
| ! | ATTGTCTGCTTTGGCTTCTA+TGG | + | Chr2:78859848-78859867 | MsG0280011047.01.T01:CDS | 40.0% |
| !! | GGTCGATGCAGATTTGTTAT+AGG | + | Chr2:78859709-78859728 | MsG0280011047.01.T01:CDS | 40.0% |
| ATAATCCTTCGTACCAGCCA+CGG | - | Chr2:78859636-78859655 | None:intergenic | 45.0% | |
| CAAGCCTTGATTCATCTTCC+GGG | + | Chr2:78859574-78859593 | MsG0280011047.01.T01:CDS | 45.0% | |
| CCATTCGGATTATCAGGATG+AGG | - | Chr2:78859786-78859805 | None:intergenic | 45.0% | |
| CCTCATCCTGATAATCCGAA+TGG | + | Chr2:78859783-78859802 | MsG0280011047.01.T01:CDS | 45.0% | |
| GTCCATCCATTCGGATTATC+AGG | - | Chr2:78859792-78859811 | None:intergenic | 45.0% | |
| TGGCTGGTACGAAGGATTAT+CGG | + | Chr2:78859636-78859655 | MsG0280011047.01.T01:CDS | 45.0% | |
| !! | AACACGTTTGTTTTCGGCGT+CGG | - | Chr2:78859520-78859539 | None:intergenic | 45.0% |
| AACGAGTAACCTCGGCATCA+TGG | + | Chr2:78859484-78859503 | MsG0280011047.01.T01:CDS | 50.0% | |
| AGGTTACTCGTTCCCATGGA+TGG | - | Chr2:78859476-78859495 | None:intergenic | 50.0% | |
| CTATAACCGTTCGTTGTCCG+TGG | + | Chr2:78859616-78859635 | MsG0280011047.01.T01:CDS | 50.0% | |
| CTGTCGTGGATGCAAAATCG+CGG | + | Chr2:78859688-78859707 | MsG0280011047.01.T01:CDS | 50.0% | |
| GCTTCCCGGAAGATGAATCA+AGG | - | Chr2:78859581-78859600 | None:intergenic | 50.0% | |
| TCCATGGGAACGAGTAACCT+CGG | + | Chr2:78859476-78859495 | MsG0280011047.01.T01:CDS | 50.0% | |
| ! | GAATTTTCGCCATGATGCCG+AGG | - | Chr2:78859496-78859515 | None:intergenic | 50.0% |
| ! | GTCTGCTTTGGCTTCTATGG+CGG | + | Chr2:78859851-78859870 | MsG0280011047.01.T01:CDS | 50.0% |
| ! | TTGAGCAGAGGTGTGCTGAT+AGG | + | Chr2:78859880-78859899 | MsG0280011047.01.T01:CDS | 50.0% |
| !! | TGGATGGACTGTTTGTCAGC+AGG | + | Chr2:78859803-78859822 | MsG0280011047.01.T01:CDS | 50.0% |
| ACGTGTCGCGTATAGCTTCC+CGG | - | Chr2:78859595-78859614 | None:intergenic | 55.0% | |
| CTGTTTGTCAGCAGGAGACG+AGG | + | Chr2:78859811-78859830 | MsG0280011047.01.T01:CDS | 55.0% | |
| GTCAGCAGGAGACGAGGATT+CGG | + | Chr2:78859817-78859836 | MsG0280011047.01.T01:CDS | 55.0% | |
| TACCAGCCACGGACAACGAA+CGG | - | Chr2:78859625-78859644 | None:intergenic | 55.0% | |
| TGGCGGAGAAAGTTGAGCAG+AGG | + | Chr2:78859868-78859887 | MsG0280011047.01.T01:CDS | 55.0% | |
| AACCGTTCGTTGTCCGTGGC+TGG | + | Chr2:78859620-78859639 | MsG0280011047.01.T01:CDS | 60.0% | |
| GCCGAGGTTACTCGTTCCCA+TGG | - | Chr2:78859480-78859499 | None:intergenic | 60.0% | |
| GTTGTCCGTGGCTGGTACGA+AGG | + | Chr2:78859628-78859647 | MsG0280011047.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 78859438 | 78859989 | 78859438 | ID=MsG0280011047.01;Name=MsG0280011047.01 |
| Chr2 | mRNA | 78859438 | 78859989 | 78859438 | ID=MsG0280011047.01.T01;Parent=MsG0280011047.01;Name=MsG0280011047.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|183 |
| Chr2 | exon | 78859438 | 78859989 | 78859438 | ID=MsG0280011047.01.T01:exon:2495;Parent=MsG0280011047.01.T01 |
| Chr2 | CDS | 78859438 | 78859989 | 78859438 | ID=MsG0280011047.01.T01:cds;Parent=MsG0280011047.01.T01 |
| Gene Sequence |
| Protein sequence |
