AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012530.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012530.01.T01 MTR_3g026330 85.437 103 15 0 1 103 190 292 6.55e-43 144
MsG0380012530.01.T01 MTR_3g026330 71.963 107 26 2 1 103 98 204 6.65e-28 105
MsG0380012530.01.T01 MTR_3g026330 58.678 121 38 4 1 113 49 165 5.07e-22 89.4
MsG0380012530.01.T01 MTR_3g026330 59.633 109 39 2 4 110 47 152 2.30e-19 82.0
MsG0380012530.01.T01 MTR_3g026330 65.217 69 22 1 3 71 271 337 1.87e-12 62.8
MsG0380012530.01.T01 MTR_3g026330 58.333 84 24 3 22 103 38 112 4.26e-12 62.0
MsG0380012530.01.T01 MTR_3g026290 79.646 113 20 1 1 113 195 304 1.69e-41 140
MsG0380012530.01.T01 MTR_3g026290 73.267 101 25 1 1 99 103 203 1.65e-28 106
MsG0380012530.01.T01 MTR_3g026290 57.812 128 50 2 1 124 68 195 5.06e-26 100
MsG0380012530.01.T01 MTR_3g026290 61.538 117 38 4 1 113 57 170 9.44e-24 94.0
MsG0380012530.01.T01 MTR_3g026290 56.489 131 50 2 1 124 149 279 2.69e-23 92.8
MsG0380012530.01.T01 MTR_3g026290 55.462 119 41 3 4 110 39 157 4.51e-20 84.0
MsG0380012530.01.T01 MTR_3g026290 48.739 119 51 3 10 124 37 149 3.69e-16 73.2
MsG0380012530.01.T01 MTR_3g026290 60.440 91 23 3 3 93 276 353 1.17e-13 66.2
MsG0380012530.01.T01 MTR_3g026290 60.563 71 26 1 1 71 285 353 1.08e-11 60.8
MsG0380012530.01.T01 MTR_3g026230 71.698 106 28 1 1 104 129 234 5.00e-32 114
MsG0380012530.01.T01 MTR_3g026230 60.584 137 41 3 1 124 107 243 1.20e-29 107
MsG0380012530.01.T01 MTR_3g026230 50.000 164 35 3 1 117 60 223 5.11e-27 100
MsG0380012530.01.T01 MTR_3g026230 67.961 103 23 2 4 104 151 245 3.54e-25 95.9
MsG0380012530.01.T01 MTR_3g026230 63.889 108 34 3 4 106 39 146 1.74e-23 91.7
MsG0380012530.01.T01 MTR_3g026230 65.138 109 31 4 1 104 49 155 1.41e-21 86.7
MsG0380012530.01.T01 MTR_3g026230 66.667 84 20 3 27 102 35 118 2.59e-15 70.1
MsG0380012530.01.T01 MTR_3g026230 69.863 73 18 2 1 71 175 245 5.92e-15 68.9
MsG0380012530.01.T01 MTR_3g026160 69.811 106 27 3 1 103 49 152 8.53e-25 93.6
MsG0380012530.01.T01 MTR_3g026160 63.333 120 37 4 1 115 60 177 1.41e-24 92.8
MsG0380012530.01.T01 MTR_3g026160 67.961 103 30 2 4 103 39 141 2.42e-24 92.4
MsG0380012530.01.T01 MTR_3g026160 58.120 117 43 3 10 123 37 150 1.38e-21 85.1
MsG0380012530.01.T01 MTR_3g026160 61.333 75 22 2 38 105 35 109 3.56e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012530.01.T01 AT5G09530 58.537 123 48 1 4 123 49 171 1.14e-28 107
MsG0380012530.01.T01 AT5G09530 51.389 144 50 2 1 124 84 227 5.27e-26 100
MsG0380012530.01.T01 AT5G09530 66.337 101 32 1 1 101 73 171 7.41e-26 100
MsG0380012530.01.T01 AT5G09530 65.347 101 33 1 2 102 41 139 3.44e-25 98.2
MsG0380012530.01.T01 AT5G09530 55.147 136 47 3 1 124 194 327 2.69e-23 93.2
MsG0380012530.01.T01 AT5G09530 56.198 121 45 2 3 117 235 353 1.84e-22 90.9
MsG0380012530.01.T01 AT5G09530 55.357 112 50 0 13 124 39 150 4.74e-22 89.7
MsG0380012530.01.T01 AT5G09530 59.130 115 38 3 3 113 257 366 5.81e-22 89.4
MsG0380012530.01.T01 AT5G09530 57.522 113 46 1 1 113 128 238 1.95e-21 87.8
MsG0380012530.01.T01 AT5G09530 52.344 128 52 2 3 126 218 340 2.80e-20 84.7
MsG0380012530.01.T01 AT5G09530 54.472 123 35 4 1 102 161 283 7.03e-19 80.9
MsG0380012530.01.T01 AT5G09530 54.615 130 40 3 1 113 150 277 7.47e-19 80.9
MsG0380012530.01.T01 AT5G09530 52.703 74 35 0 51 124 33 106 1.11e-11 60.8
MsG0380012530.01.T01 AT5G09520 54.545 99 38 2 2 100 39 130 2.37e-16 70.5
MsG0380012530.01.T01 AT5G09520 53.333 90 37 1 13 102 37 121 2.94e-14 65.1
MsG0380012530.01.T01 AT5G09520 55.696 79 30 1 26 104 39 112 4.37e-14 64.3
MsG0380012530.01.T01 AT5G09520 61.111 72 25 2 37 105 39 110 1.55e-13 63.2
MsG0380012530.01.T01 AT5G09520 61.667 60 23 0 46 105 37 96 1.15e-12 60.8
MsG0380012530.01.T01 AT5G09520 61.538 52 20 0 51 102 31 82 7.34e-11 56.2

Find 45 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCGGGGACTTTAGGGAGTT+TGG 0.229602 3:-19299139 None:intergenic
TTAGGTAACTCGGGGACTTT+AGG 0.244084 3:-19299147 None:intergenic
TTAGGTAATTCAGGAAATTT+AGG 0.260109 3:-19299082 None:intergenic
TCAGGTACCTCAGGGGCTTT+AGG 0.279754 3:-19298917 None:intergenic
AGCTCGAGTTTCGGCAGTTC+AGG 0.287779 3:-19298827 None:intergenic
CAACTCAGGTTAGGTAATTC+AGG 0.297210 3:-19299091 None:intergenic
TTAGGGAATTCAGGGGCTTT+AGG 0.299732 3:-19298851 None:intergenic
TTAGGTAATTCAGGAACTTT+AGG 0.308829 3:-19299049 None:intergenic
AATTTAGGCAACTCAGGTTT+AGG 0.326974 3:-19299067 None:intergenic
ACATCCGACTTAGGGAATTC+AGG 0.330532 3:-19298860 None:intergenic
AACTTAGGTAGTTCAGGCTT+TGG 0.356849 3:-19298797 None:intergenic
ACATTAAACTTAGGTAGTTC+AGG 0.365954 3:-19298803 None:intergenic
TAGGTAACTCGGGGACTTTA+GGG 0.367520 3:-19299146 None:intergenic
AACTCAGGTTTAGGTAATTC+AGG 0.368433 3:-19299058 None:intergenic
TCGGGGACTTTAGGGAGTTT+GGG 0.388929 3:-19299138 None:intergenic
ACCTTAGGCTGCTCTAGTTT+AGG 0.391315 3:-19299165 None:intergenic
GCTTTAGGAAGCTCGTGTTT+TGG 0.406146 3:-19298902 None:intergenic
ACCTTAGATAGCCCTGGTTC+AGG 0.407392 3:-19298935 None:intergenic
TTTGGAAGTTTAGGAACCTT+AGG 0.421155 3:-19298884 None:intergenic
ACCTGAACCAGGGCTATCTA+AGG 0.429236 3:+19298934 MsG0380012530.01.T01:CDS
AGCCCTGGTTCAGGTACCTC+AGG 0.431178 3:-19298926 None:intergenic
GCCCCTGAGGTACCTGAACC+AGG 0.441907 3:+19298923 MsG0380012530.01.T01:CDS
CATCCGACTTAGGGAATTCA+GGG 0.455443 3:-19298859 None:intergenic
CTGCTCTAGTTTAGGTAACT+CGG 0.471107 3:-19299157 None:intergenic
TGCTCTAGTTTAGGTAACTC+GGG 0.474750 3:-19299156 None:intergenic
CCCTAAGTCGGATGTTCCTA+AGG 0.483588 3:+19298868 MsG0380012530.01.T01:CDS
AATCCTAGGCAACTCAGGTT+AGG 0.485340 3:-19299100 None:intergenic
TCAGGAAATTTAGGCAACTC+AGG 0.496130 3:-19299073 None:intergenic
CCTTAGGAACATCCGACTTA+GGG 0.496338 3:-19298868 None:intergenic
ACCTTAGGAACATCCGACTT+AGG 0.497442 3:-19298869 None:intergenic
GCTTTAGGAAGCTCGAGTTT+CGG 0.497852 3:-19298836 None:intergenic
TCAGGAACTTTAGGCAACTC+AGG 0.512033 3:-19299040 None:intergenic
AGTTCAGGAACATTAAACTT+AGG 0.512127 3:-19298812 None:intergenic
AATTGTGGCTTAGGTAACTC+AGG 0.521409 3:-19298992 None:intergenic
GCCCTGGTTCAGGTACCTCA+GGG 0.523768 3:-19298925 None:intergenic
TTACCTAACCTGAGTTGCCT+AGG 0.524197 3:+19299097 MsG0380012530.01.T01:CDS
TCAGAAACCTTAGATAGCCC+TGG 0.540598 3:-19298941 None:intergenic
TCGGAAATCCTAGGCAACTC+AGG 0.548546 3:-19299105 None:intergenic
AGCCCCTGAATTCCCTAAGT+CGG 0.589665 3:+19298856 MsG0380012530.01.T01:CDS
CCCCTGAGGTACCTGAACCA+GGG 0.624156 3:+19298924 MsG0380012530.01.T01:CDS
ATTGTGGCTTAGGTAACTCA+GGG 0.633142 3:-19298991 None:intergenic
CCCTGGTTCAGGTACCTCAG+GGG 0.652309 3:-19298924 None:intergenic
GCTCTAGTTTAGGTAACTCG+GGG 0.677089 3:-19299155 None:intergenic
ATCCGACTTAGGGAATTCAG+GGG 0.692397 3:-19298858 None:intergenic
CGAGCTTCCTAAAGCCCCTG+AGG 0.703869 3:+19298910 MsG0380012530.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTAGGTAATTCAGGAAATTT+AGG - Chr3:19299085-19299104 None:intergenic 25.0%
ACATTAAACTTAGGTAGTTC+AGG - Chr3:19298806-19298825 None:intergenic 30.0%
AGTTCAGGAACATTAAACTT+AGG - Chr3:19298815-19298834 None:intergenic 30.0%
TTAGGTAATTCAGGAACTTT+AGG - Chr3:19299052-19299071 None:intergenic 30.0%
! TTTGTCAATTCAGGAGTTTT+AGG - Chr3:19299019-19299038 None:intergenic 30.0%
!! ATTTTAGGCAATTCAGGTTT+TGG - Chr3:19298971-19298990 None:intergenic 30.0%
AACTCAGGTTTAGGTAATTC+AGG - Chr3:19299061-19299080 None:intergenic 35.0%
AATTTAGGCAACTCAGGTTT+AGG - Chr3:19299070-19299089 None:intergenic 35.0%
TTTGGAAGTTTAGGAACCTT+AGG - Chr3:19298887-19298906 None:intergenic 35.0%
! TTAGGTAACTCAGGGATTTT+AGG - Chr3:19298986-19299005 None:intergenic 35.0%
! TTTTGCATTTCGGAAATCCT+AGG - Chr3:19299117-19299136 None:intergenic 35.0%
!! AACTCAGGTTTTGTCAATTC+AGG - Chr3:19299028-19299047 None:intergenic 35.0%
!! GAGTTTGGGTTTTTGCATTT+CGG - Chr3:19299127-19299146 None:intergenic 35.0%
AACTTAGGTAGTTCAGGCTT+TGG - Chr3:19298800-19298819 None:intergenic 40.0%
AATTGTGGCTTAGGTAACTC+AGG - Chr3:19298995-19299014 None:intergenic 40.0%
ATTGTGGCTTAGGTAACTCA+GGG - Chr3:19298994-19299013 None:intergenic 40.0%
CAACTCAGGTTAGGTAATTC+AGG - Chr3:19299094-19299113 None:intergenic 40.0%
TCAGGAAATTTAGGCAACTC+AGG - Chr3:19299076-19299095 None:intergenic 40.0%
! TCAGGGATTTTAGGCAATTC+AGG - Chr3:19298977-19298996 None:intergenic 40.0%
!! GTTTTAGGCAATTGTGGCTT+AGG - Chr3:19299004-19299023 None:intergenic 40.0%
!!! AGCTCGTGTTTTGGAAGTTT+AGG - Chr3:19298896-19298915 None:intergenic 40.0%
!!! TCAGGAGTTTTAGGCAATTG+TGG - Chr3:19299010-19299029 None:intergenic 40.0%
AATCCTAGGCAACTCAGGTT+AGG - Chr3:19299103-19299122 None:intergenic 45.0%
ACATCCGACTTAGGGAATTC+AGG - Chr3:19298863-19298882 None:intergenic 45.0%
ACCTTAGGAACATCCGACTT+AGG - Chr3:19298872-19298891 None:intergenic 45.0%
ATCCGACTTAGGGAATTCAG+GGG - Chr3:19298861-19298880 None:intergenic 45.0%
CATCCGACTTAGGGAATTCA+GGG - Chr3:19298862-19298881 None:intergenic 45.0%
CCTTAGGAACATCCGACTTA+GGG - Chr3:19298871-19298890 None:intergenic 45.0%
TAGGTAACTCGGGGACTTTA+GGG - Chr3:19299149-19299168 None:intergenic 45.0%
TCAGAAACCTTAGATAGCCC+TGG - Chr3:19298944-19298963 None:intergenic 45.0%
TCAGGAACTTTAGGCAACTC+AGG - Chr3:19299043-19299062 None:intergenic 45.0%
TTACCTAACCTGAGTTGCCT+AGG + Chr3:19299097-19299116 MsG0380012530.01.T01:CDS 45.0%
TTAGGGAATTCAGGGGCTTT+AGG - Chr3:19298854-19298873 None:intergenic 45.0%
TTAGGTAACTCGGGGACTTT+AGG - Chr3:19299150-19299169 None:intergenic 45.0%
!! GCTTTAGGAAGCTCGAGTTT+CGG - Chr3:19298839-19298858 None:intergenic 45.0%
!!! GCTTTAGGAAGCTCGTGTTT+TGG - Chr3:19298905-19298924 None:intergenic 45.0%
ACCTGAACCAGGGCTATCTA+AGG + Chr3:19298934-19298953 MsG0380012530.01.T01:CDS 50.0%
ACCTTAGATAGCCCTGGTTC+AGG - Chr3:19298938-19298957 None:intergenic 50.0%
AGCCCCTGAATTCCCTAAGT+CGG + Chr3:19298856-19298875 MsG0380012530.01.T01:CDS 50.0%
CCCTAAGTCGGATGTTCCTA+AGG + Chr3:19298868-19298887 MsG0380012530.01.T01:CDS 50.0%
TCGGAAATCCTAGGCAACTC+AGG - Chr3:19299108-19299127 None:intergenic 50.0%
! TCGGGGACTTTAGGGAGTTT+GGG - Chr3:19299141-19299160 None:intergenic 50.0%
AGCTCGAGTTTCGGCAGTTC+AGG - Chr3:19298830-19298849 None:intergenic 55.0%
TCAGGTACCTCAGGGGCTTT+AGG - Chr3:19298920-19298939 None:intergenic 55.0%
! CTCGGGGACTTTAGGGAGTT+TGG - Chr3:19299142-19299161 None:intergenic 55.0%
AGCCCTGGTTCAGGTACCTC+AGG - Chr3:19298929-19298948 None:intergenic 60.0%
CCCTGGTTCAGGTACCTCAG+GGG - Chr3:19298927-19298946 None:intergenic 60.0%
CGAGCTTCCTAAAGCCCCTG+AGG + Chr3:19298844-19298863 MsG0380012530.01.T01:CDS 60.0%
GCCCTGGTTCAGGTACCTCA+GGG - Chr3:19298928-19298947 None:intergenic 60.0%
! CCCCTGAGGTACCTGAACCA+GGG + Chr3:19298924-19298943 MsG0380012530.01.T01:CDS 60.0%
! GCCCCTGAGGTACCTGAACC+AGG + Chr3:19298923-19298942 MsG0380012530.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 19298794 19299174 19298794 ID=MsG0380012530.01;Name=MsG0380012530.01
Chr3 mRNA 19298794 19299174 19298794 ID=MsG0380012530.01.T01;Parent=MsG0380012530.01;Name=MsG0380012530.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|126
Chr3 exon 19298794 19299174 19298794 ID=MsG0380012530.01.T01:exon:7505;Parent=MsG0380012530.01.T01
Chr3 CDS 19298794 19299174 19298794 ID=MsG0380012530.01.T01:cds;Parent=MsG0380012530.01.T01
Gene Sequence

>MsG0380012530.01.T01

ATGCCAAAGCCTGAACTACCTAAGTTTAATGTTCCTGAACTGCCGAAACTCGAGCTTCCTAAAGCCCCTGAATTCCCTAAGTCGGATGTTCCTAAGGTTCCTAAACTTCCAAAACACGAGCTTCCTAAAGCCCCTGAGGTACCTGAACCAGGGCTATCTAAGGTTTCTGAATTGCCAAAACCTGAATTGCCTAAAATCCCTGAGTTACCTAAGCCACAATTGCCTAAAACTCCTGAATTGACAAAACCTGAGTTGCCTAAAGTTCCTGAATTACCTAAACCTGAGTTGCCTAAATTTCCTGAATTACCTAACCTGAGTTGCCTAGGATTTCCGAAATGCAAAAACCCAAACTCCCTAAAGTCCCCGAGTTACCTAAACTAG
Protein sequence

>MsG0380012530.01.T01

MPKPELPKFNVPELPKLELPKAPEFPKSDVPKVPKLPKHELPKAPEVPEPGLSKVSELPKPELPKIPELPKPQLPKTPELTKPELPKVPELPKPELPKFPELPNLSCLGFPKCKNPNSLKSPSYLN*