Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012560.01.T01 | XP_039687807.1 | 79.021 | 143 | 23 | 3 | 4 | 142 | 68 | 207 | 3.08E-68 | 222 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012560.01.T01 | Q9FME3 | 84.444 | 45 | 7 | 0 | 4 | 48 | 74 | 118 | 7.60E-18 | 82.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012560.01.T01 | G7IZC4 | 79.021 | 143 | 23 | 3 | 4 | 142 | 68 | 207 | 1.47e-68 | 222 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280008939.01 | MsG0380012560.01 | 0.829542 | 4.856550e-55 | 4.985393e-52 |
| MsG0280008966.01 | MsG0380012560.01 | 0.813066 | 3.089353e-51 | 1.997085e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012560.01.T01 | MTR_3g026050 | 79.021 | 143 | 23 | 3 | 4 | 142 | 68 | 207 | 3.73e-72 | 222 |
| MsG0380012560.01.T01 | MTR_7g015010 | 77.778 | 45 | 10 | 0 | 4 | 48 | 68 | 112 | 1.66e-18 | 81.3 |
| MsG0380012560.01.T01 | MTR_4g109660 | 77.778 | 45 | 10 | 0 | 4 | 48 | 67 | 111 | 2.03e-18 | 80.5 |
| MsG0380012560.01.T01 | MTR_6g015350 | 69.388 | 49 | 14 | 1 | 4 | 52 | 60 | 107 | 2.28e-14 | 69.7 |
| MsG0380012560.01.T01 | MTR_2g090960 | 59.524 | 42 | 17 | 0 | 4 | 45 | 35 | 76 | 7.70e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012560.01.T01 | AT5G60970 | 84.444 | 45 | 7 | 0 | 4 | 48 | 74 | 118 | 7.75e-19 | 82.4 |
| MsG0380012560.01.T01 | AT5G08070 | 67.925 | 53 | 15 | 1 | 4 | 54 | 44 | 96 | 7.29e-16 | 72.8 |
| MsG0380012560.01.T01 | AT5G08070 | 67.925 | 53 | 15 | 1 | 4 | 54 | 44 | 96 | 7.29e-16 | 72.8 |
| MsG0380012560.01.T01 | AT3G02150 | 68.889 | 45 | 14 | 0 | 4 | 48 | 85 | 129 | 7.74e-16 | 73.9 |
| MsG0380012560.01.T01 | AT3G02150 | 72.093 | 43 | 12 | 0 | 6 | 48 | 87 | 129 | 7.82e-16 | 73.2 |
| MsG0380012560.01.T01 | AT4G18390 | 52.542 | 59 | 23 | 1 | 1 | 54 | 50 | 108 | 1.43e-13 | 67.4 |
| MsG0380012560.01.T01 | AT4G18390 | 52.542 | 59 | 23 | 1 | 1 | 54 | 50 | 108 | 1.43e-13 | 67.4 |
| MsG0380012560.01.T01 | AT1G30210 | 68.182 | 44 | 14 | 0 | 1 | 44 | 58 | 101 | 2.00e-13 | 67.0 |
| MsG0380012560.01.T01 | AT1G30210 | 68.182 | 44 | 14 | 0 | 1 | 44 | 58 | 101 | 2.00e-13 | 67.0 |
| MsG0380012560.01.T01 | AT1G53230 | 61.905 | 42 | 16 | 0 | 4 | 45 | 60 | 101 | 5.10e-12 | 63.2 |
| MsG0380012560.01.T01 | AT1G53230 | 61.905 | 42 | 16 | 0 | 4 | 45 | 60 | 101 | 5.10e-12 | 63.2 |
| MsG0380012560.01.T01 | AT2G31070 | 59.524 | 42 | 17 | 0 | 4 | 45 | 40 | 81 | 7.02e-11 | 59.7 |
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATGGCAAGGTCAAAATTT+TGG | 0.095121 | 3:-19738360 | MsG0380012560.01.T01:CDS |
| ATTCCAACAAATGTGGAATT+AGG | 0.207117 | 3:+19738391 | None:intergenic |
| TGTTGGAATTCAAAATCTTA+TGG | 0.268566 | 3:-19738377 | MsG0380012560.01.T01:CDS |
| CTTCAAGATAAACTTGGTTT+AGG | 0.292497 | 3:-19738598 | MsG0380012560.01.T01:CDS |
| AGCAAAGTTTGTACAATAAG+AGG | 0.341408 | 3:-19738675 | None:intergenic |
| GATCCTAATTCCACATTTGT+TGG | 0.359932 | 3:-19738394 | MsG0380012560.01.T01:CDS |
| AACCAATCAATTACTTTACT+TGG | 0.384521 | 3:+19738571 | None:intergenic |
| GCTTCTCATGAACTTTCTCT+TGG | 0.400555 | 3:-19738427 | MsG0380012560.01.T01:CDS |
| AAGAGAGTACTTTAGTGAAA+AGG | 0.409808 | 3:-19738302 | MsG0380012560.01.T01:CDS |
| AACCAAGTAAAGTAATTGAT+TGG | 0.421109 | 3:-19738573 | MsG0380012560.01.T01:CDS |
| ATTGATCATGAAAAGGAAGT+AGG | 0.421778 | 3:+19738461 | None:intergenic |
| ATAAGAGGTTAAGAGATAGA+AGG | 0.427344 | 3:-19738660 | MsG0380012560.01.T01:CDS |
| GTGGAATTAGGATCATATAA+AGG | 0.445075 | 3:+19738403 | None:intergenic |
| TCATATAATTGAATTGCTGT+TGG | 0.472619 | 3:+19738622 | None:intergenic |
| TTACTTGATTGATCATGAAA+AGG | 0.475662 | 3:+19738454 | None:intergenic |
| GGTTAAGAGATAGAAGGATA+AGG | 0.482214 | 3:-19738654 | MsG0380012560.01.T01:CDS |
| TTGATCATGAAAAGGAAGTA+GGG | 0.513383 | 3:+19738462 | None:intergenic |
| GACATAGATTCAAGGACTAA+AGG | 0.516620 | 3:-19738337 | MsG0380012560.01.T01:CDS |
| TATGATCTTCAAGATAAACT+TGG | 0.527365 | 3:-19738604 | MsG0380012560.01.T01:CDS |
| AATGAGGAAGAAAATCAAGT+TGG | 0.535305 | 3:-19738263 | MsG0380012560.01.T01:CDS |
| AAATTAGAATGAGTCTCCAT+AGG | 0.541479 | 3:+19738192 | None:intergenic |
| AATTAGAATGAGTCTCCATA+GGG | 0.543229 | 3:+19738193 | None:intergenic |
| ATGAAATTGTGAAAAGCTTG+AGG | 0.549364 | 3:+19738500 | None:intergenic |
| GCTTGAGGAAATTGAAGTGG+AGG | 0.556558 | 3:+19738515 | None:intergenic |
| ACTTTAGTGAAAAGGATAAG+TGG | 0.567465 | 3:-19738294 | MsG0380012560.01.T01:CDS |
| GAATTCAAAATCTTATGGCA+AGG | 0.581024 | 3:-19738372 | MsG0380012560.01.T01:CDS |
| AAGTAGGGTTGTGAATGAAG+AGG | 0.590488 | 3:+19738477 | None:intergenic |
| CTTGAGGAAATTGAAGTGGA+GGG | 0.601006 | 3:+19738516 | None:intergenic |
| AAAGCTTGAGGAAATTGAAG+TGG | 0.628614 | 3:+19738512 | None:intergenic |
| ATAAGTGGATCAAAACAATG+AGG | 0.688963 | 3:-19738279 | MsG0380012560.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACCAAGTAAAGTAATTGAT+TGG | - | Chr3:19738289-19738308 | MsG0380012560.01.T01:CDS | 25.0% |
| ! | AACCAATCAATTACTTTACT+TGG | + | Chr3:19738294-19738313 | None:intergenic | 25.0% |
| ! | TATGATCTTCAAGATAAACT+TGG | - | Chr3:19738258-19738277 | MsG0380012560.01.T01:CDS | 25.0% |
| ! | TCATATAATTGAATTGCTGT+TGG | + | Chr3:19738243-19738262 | None:intergenic | 25.0% |
| ! | TGTTGGAATTCAAAATCTTA+TGG | - | Chr3:19738485-19738504 | MsG0380012560.01.T01:CDS | 25.0% |
| ! | TTACTTGATTGATCATGAAA+AGG | + | Chr3:19738411-19738430 | None:intergenic | 25.0% |
| !! | ATTTTGAATTCCAACAAATG+TGG | + | Chr3:19738481-19738500 | None:intergenic | 25.0% |
| AATGAGGAAGAAAATCAAGT+TGG | - | Chr3:19738599-19738618 | MsG0380012560.01.T01:CDS | 30.0% | |
| ACTTTAGTGAAAAGGATAAG+TGG | - | Chr3:19738568-19738587 | MsG0380012560.01.T01:CDS | 30.0% | |
| ATAAGAGGTTAAGAGATAGA+AGG | - | Chr3:19738202-19738221 | MsG0380012560.01.T01:CDS | 30.0% | |
| ATAAGTGGATCAAAACAATG+AGG | - | Chr3:19738583-19738602 | MsG0380012560.01.T01:CDS | 30.0% | |
| ATGAAATTGTGAAAAGCTTG+AGG | + | Chr3:19738365-19738384 | None:intergenic | 30.0% | |
| ATTCCAACAAATGTGGAATT+AGG | + | Chr3:19738474-19738493 | None:intergenic | 30.0% | |
| ATTGATCATGAAAAGGAAGT+AGG | + | Chr3:19738404-19738423 | None:intergenic | 30.0% | |
| CTTCAAGATAAACTTGGTTT+AGG | - | Chr3:19738264-19738283 | MsG0380012560.01.T01:CDS | 30.0% | |
| GAATTCAAAATCTTATGGCA+AGG | - | Chr3:19738490-19738509 | MsG0380012560.01.T01:CDS | 30.0% | |
| GTGGAATTAGGATCATATAA+AGG | + | Chr3:19738462-19738481 | None:intergenic | 30.0% | |
| TTGATCATGAAAAGGAAGTA+GGG | + | Chr3:19738403-19738422 | None:intergenic | 30.0% | |
| ! | TATGGCAAGGTCAAAATTTT+GGG | - | Chr3:19738503-19738522 | MsG0380012560.01.T01:CDS | 30.0% |
| ! | TTATGGCAAGGTCAAAATTT+TGG | - | Chr3:19738502-19738521 | MsG0380012560.01.T01:CDS | 30.0% |
| !! | AAGAGAGTACTTTAGTGAAA+AGG | - | Chr3:19738560-19738579 | MsG0380012560.01.T01:CDS | 30.0% |
| !!! | AATTTTGGGACATAGATTCA+AGG | - | Chr3:19738517-19738536 | MsG0380012560.01.T01:CDS | 30.0% |
| AAAGCTTGAGGAAATTGAAG+TGG | + | Chr3:19738353-19738372 | None:intergenic | 35.0% | |
| GACATAGATTCAAGGACTAA+AGG | - | Chr3:19738525-19738544 | MsG0380012560.01.T01:CDS | 35.0% | |
| GATCCTAATTCCACATTTGT+TGG | - | Chr3:19738468-19738487 | MsG0380012560.01.T01:CDS | 35.0% | |
| GGTTAAGAGATAGAAGGATA+AGG | - | Chr3:19738208-19738227 | MsG0380012560.01.T01:CDS | 35.0% | |
| ! | AACTCAAAAGCTTTTCCCTA+TGG | - | Chr3:19738654-19738673 | MsG0380012560.01.T01:CDS | 35.0% |
| AAGTAGGGTTGTGAATGAAG+AGG | + | Chr3:19738388-19738407 | None:intergenic | 40.0% | |
| CTTGAGGAAATTGAAGTGGA+GGG | + | Chr3:19738349-19738368 | None:intergenic | 40.0% | |
| GCTTCTCATGAACTTTCTCT+TGG | - | Chr3:19738435-19738454 | MsG0380012560.01.T01:CDS | 40.0% | |
| GCTTGAGGAAATTGAAGTGG+AGG | + | Chr3:19738350-19738369 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 19738195 | 19738689 | 19738195 | ID=MsG0380012560.01;Name=MsG0380012560.01 |
| Chr3 | mRNA | 19738195 | 19738689 | 19738195 | ID=MsG0380012560.01.T01;Parent=MsG0380012560.01;Name=MsG0380012560.01.T01;_AED=0.27;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|164 |
| Chr3 | exon | 19738195 | 19738689 | 19738195 | ID=MsG0380012560.01.T01:exon:3797;Parent=MsG0380012560.01.T01 |
| Chr3 | CDS | 19738195 | 19738689 | 19738195 | ID=MsG0380012560.01.T01:cds;Parent=MsG0380012560.01.T01 |
| Gene Sequence |
| Protein sequence |
