AlfalfaGEDB | MsG0380012609.01

M. sativa cultivar ZhongmuNo.1 / MsG0380012609.01

KEGG: -
GO: GO:0046983
PFAM: PF00010

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012609.01.T01	XP_003599090.2	94.161	411	15	2	117	523	1	406	0	793

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012609.01.T01	Q66GR3	34.236	406	200	14	130	517	1	357	7.90E-46	167

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012609.01.T01	G7IWI2	94.161	411	15	2	117	523	1	406	0.0	793

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TFs/TRs:

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Gene ID	Type	Classification
MsG0380012609.01.T01	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380012609.01	MsG0680031814.01	0.821317	4.305528e-53	3.490973e-50

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PPI

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Gene1	Gene2	Type
MsG0380012609.01	MsG0480021094.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012609.01.T01	MTR_3g028520	94.161	411	15	2	117	523	1	406	0.0	793
MsG0380012609.01.T01	MTR_3g027650	94.161	411	15	2	117	523	1	406	0.0	793
MsG0380012609.01.T01	MTR_3g027650	94.087	389	14	2	117	501	1	384	0.0	743
MsG0380012609.01.T01	MTR_3g027650	93.973	365	13	2	117	477	1	360	0.0	696
MsG0380012609.01.T01	MTR_8g036235	51.111	405	149	10	126	513	18	390	1.71e-105	321
MsG0380012609.01.T01	MTR_8g036235	49.322	369	138	10	126	477	18	354	2.09e-84	267
MsG0380012609.01.T01	MTR_2g098310	37.204	422	213	18	134	517	3	410	2.29e-51	181
MsG0380012609.01.T01	MTR_1g052470	37.349	415	182	18	130	511	1	370	2.67e-51	180
MsG0380012609.01.T01	MTR_4g065870	33.860	443	215	16	130	517	13	432	3.17e-47	170
MsG0380012609.01.T01	MTR_2g098310	37.010	408	205	18	134	503	3	396	1.79e-45	165

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012609.01.T01	AT2G42280	34.236	406	200	14	130	517	1	357	8.05e-47	167
MsG0380012609.01.T01	AT1G51140	32.732	388	224	12	137	513	17	378	2.81e-41	152
MsG0380012609.01.T01	AT2G42280	33.333	390	193	14	130	501	1	341	5.31e-40	149
MsG0380012609.01.T01	AT2G43140	50.336	149	59	4	371	517	77	212	1.38e-37	138
MsG0380012609.01.T01	AT2G43140	50.676	148	60	4	371	517	174	309	1.00e-36	138
MsG0380012609.01.T01	AT1G05805	34.184	392	187	16	144	516	21	360	1.45e-36	139
MsG0380012609.01.T01	AT1G35460	65.517	87	30	0	431	517	173	259	1.28e-32	125
MsG0380012609.01.T01	AT4G09180	50.000	140	58	3	380	517	133	262	1.50e-31	123
MsG0380012609.01.T01	AT2G42280	28.696	345	179	14	130	456	1	296	2.86e-17	83.2
MsG0380012609.01.T01	AT1G35460	72.000	50	14	0	431	480	173	222	1.32e-16	79.7

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Find 110 sgRNAs with CRISPR-Local

Find 340 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCTACTAGTAGTGCA+CGG	0.677439	3:-20869071	MsG0380012609.01.T01:CDS
AAAATTCTGGACTATTGATG+AGG	0.635672	3:-20865445	MsG0380012609.01.T01:CDS
AAAGCCAATGGCATCTCAAA+TGG	0.469024	3:-20864130	MsG0380012609.01.T01:CDS
AAAGGATTAAATCAGCTACA+AGG	0.569777	3:+20862960	None:intergenic
AAATCAGCTACAAGGTTTAC+AGG	0.285709	3:+20862968	None:intergenic
AACAAGCACGGCAGATATGT+TGG	0.496382	3:-20863199	MsG0380012609.01.T01:CDS
AACATTAAATATACTTACAT+TGG	0.481912	3:+20868732	None:intergenic
AAGCCTGTGAAGCAAGAAAT+AGG	0.413405	3:-20865224	MsG0380012609.01.T01:CDS
AAGTTGATATCATCCAACAA+TGG	0.670047	3:-20865287	MsG0380012609.01.T01:CDS
AATCAGCTACAAGGTTTACA+GGG	0.586955	3:+20862969	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTCTACTAGTAGTGCA+CGG	-	Chr3:20862721-20862740	MsG0380012609.01.T01:three_prime_UTR	30.0%
AAAATTCTGGACTATTGATG+AGG	-	Chr3:20866347-20866366	MsG0380012609.01.T01:intron	30.0%
AAAGGATTAAATCAGCTACA+AGG	+	Chr3:20868835-20868854	None:intergenic	30.0%
AAAGTTATCAAGCCATTCTT+AGG	-	Chr3:20863564-20863583	MsG0380012609.01.T01:CDS	30.0%
AACAGACCCTAATTATTAAG+AGG	+	Chr3:20866872-20866891	None:intergenic	30.0%
AATCTGCTTAACATACTTGT+AGG	-	Chr3:20869112-20869131	MsG0380012609.01.T01:CDS	30.0%
AATGTTAATGTGAGGTCTTA+AGG	-	Chr3:20869144-20869163	MsG0380012609.01.T01:CDS	30.0%
AATTAGACTCAAACATCCAA+AGG	+	Chr3:20865948-20865967	None:intergenic	30.0%
AATTCTACTGATATTGTGTC+GGG	+	Chr3:20862875-20862894	None:intergenic	30.0%
AGAATTGTCCCAAAATTCAT+TGG	+	Chr3:20867840-20867859	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	20862651	20869163	20862651	ID=MsG0380012609.01;Name=MsG0380012609.01
Chr3	mRNA	20862651	20869163	20862651	ID=MsG0380012609.01.T01;Parent=MsG0380012609.01;Name=MsG0380012609.01.T01;_AED=0.27;_eAED=0.27;_QI=0\|0.66\|0.57\|1\|0.83\|0.71\|7\|320\|523
Chr3	exon	20862651	20863042	20862651	ID=MsG0380012609.01.T01:exon:7776;Parent=MsG0380012609.01.T01
Chr3	exon	20863155	20863223	20863155	ID=MsG0380012609.01.T01:exon:7775;Parent=MsG0380012609.01.T01
Chr3	exon	20863545	20863610	20863545	ID=MsG0380012609.01.T01:exon:7774;Parent=MsG0380012609.01.T01
Chr3	exon	20863712	20864190	20863712	ID=MsG0380012609.01.T01:exon:7773;Parent=MsG0380012609.01.T01
Chr3	exon	20865003	20865581	20865003	ID=MsG0380012609.01.T01:exon:7772;Parent=MsG0380012609.01.T01
Chr3	exon	20868746	20868836	20868746	ID=MsG0380012609.01.T01:exon:7771;Parent=MsG0380012609.01.T01
Chr3	exon	20868948	20869163	20868948	ID=MsG0380012609.01.T01:exon:7770;Parent=MsG0380012609.01.T01
Chr3	CDS	20868948	20869163	20868948	ID=MsG0380012609.01.T01:cds;Parent=MsG0380012609.01.T01
Chr3	CDS	20868746	20868836	20868746	ID=MsG0380012609.01.T01:cds;Parent=MsG0380012609.01.T01
Chr3	CDS	20865003	20865581	20865003	ID=MsG0380012609.01.T01:cds;Parent=MsG0380012609.01.T01
Chr3	CDS	20863712	20864190	20863712	ID=MsG0380012609.01.T01:cds;Parent=MsG0380012609.01.T01
Chr3	CDS	20863545	20863610	20863545	ID=MsG0380012609.01.T01:cds;Parent=MsG0380012609.01.T01
Chr3	CDS	20863155	20863223	20863155	ID=MsG0380012609.01.T01:cds;Parent=MsG0380012609.01.T01
Chr3	CDS	20862971	20863042	20862971	ID=MsG0380012609.01.T01:cds;Parent=MsG0380012609.01.T01
Chr3	three_prime_UTR	20862651	20862970	20862651	ID=MsG0380012609.01.T01:three_prime_utr;Parent=MsG0380012609.01.T01

Gene Sequence

>MsG0380012609.01.T01

ATGTCTCGGAGGAATACGTCTCAGAGGCTACCTGAGGTGACGAGTGGCAGACTTAGGTTAGGGAGAGTACAAAATTCTACTAGTAGTGCACGGAGGGAGAAGCAAGCAGTACGCCCTCCTTTGGCTGGTCGAGGGAGAGGCAAACTGGGGGGCACTGGAGTTGGTAGGGTTGATGCTGCGGGGGACTCATCATCACAGACGGGTCGATCCCAGACGTATGAGGATCATCAGGAGCAGCCTGATATTGAGCAGGAGGGTGGGGGTCATGCTCAGGGGGTGCCGAGGGTGGAGTACGAGGCCAATGTAAATGTTTTTTTTTTTTTTTATGCAGATGATTGTATAAGCAAGATGGGTGCTATGTATGAAGAGCTAAGAAGAAACCAAGAAATGGATTCTTCAAGTATTATTCATTCAAAACAACAAAATTCTGGACTATTGATGAGGTACAATTCTGCTCCAACCTCATTGTTTACAAACCTAATCGACAACAACGCTCAAAACTATATCAACAACGAAGAATCGTTTACAAACGAGAGTTATATTCCATCAACAAGTTCAGAAATGGATACCATGTTATCCAAGTTGATATCATCCAACAATGGATGGAACAATTCTGAAGAAAATTTGCAAGAGTTTGATGTGAAGCCTGTGAAGCAAGAAATAGGTGAATCTGTTGCACAACAGAATGGTAATTATTCTTATGGTGGATCTGAGTTGATTTACCAAGGCTTTTCAAATGGTTCTGGCAATGGTTTTTATGGCTCTTTTGGTGGAGTTGATACTATGGAATCAGATGATTCCAATCAATCTAAAATTGGTGTTAGGAATTGCTCCAATCTTATTAGGCAAAAGAGTTCCCCTGCTGAGTTTTTCTCTAATGAAAATGGACTTGCAACATTGACATTGAGAGAGGTAGAAAGCTTTAAAGCCAATGGCATCTCAAATGGTACACTTAACTTCTCATCTAGGCCATCTACTAGCTTGAAAAGGATGCCACAAATAGCTGAAAATGGAATTCAAAGCTTAGAACCAAATTGTGACCAAACTACAAACCTAGTAAATGAAAATGGTAGCTCCAAAAGTCACATACCTAGCTTCACCAATGAATTTTGGGACAATTCTGCATTCAATGCACACAAAACAGAGAGTGAAGATGAAATAATGTTTTCTACTTCAAATGGTTTGGACTCTCAAGAAGCAGATTTTGGCTATCAAAATCTTGGTTTGACTAGTCATCTGAGTCTTCCTAGTTCTTCTACTAAGATGACATCTATGGAGAATTTTCTTGAAATTCAAGGTTCTGTTCCTTGTAAAATTCGAGCGAAAAGAGGGTTTGCAACTCATCCAAGAAGCATTGCAGAGAGGGTCAGAAGAACCAGAATTAGTGACAGAATCAAGAAACTGCAAGGTCTTTTCCCAAAATCAGATAAGCAAACAAGCACGGCAGATATGTTGGATTTGGCAGTTGAGTACATTAAAGACCTACAAGAACAAGTTCAGATACTCACAGATTGTAAGGAAAAATGCAAATGTGCAAGTCATGAGAAGAAACCCTGTAAACCTTGTAGCTGA

Protein sequence

>MsG0380012609.01.T01

MSRRNTSQRLPEVTSGRLRLGRVQNSTSSARREKQAVRPPLAGRGRGKLGGTGVGRVDAAGDSSSQTGRSQTYEDHQEQPDIEQEGGGHAQGVPRVEYEANVNVFFFFYADDCISKMGAMYEELRRNQEMDSSSIIHSKQQNSGLLMRYNSAPTSLFTNLIDNNAQNYINNEESFTNESYIPSTSSEMDTMLSKLISSNNGWNNSEENLQEFDVKPVKQEIGESVAQQNGNYSYGGSELIYQGFSNGSGNGFYGSFGGVDTMESDDSNQSKIGVRNCSNLIRQKSSPAEFFSNENGLATLTLREVESFKANGISNGTLNFSSRPSTSLKRMPQIAENGIQSLEPNCDQTTNLVNENGSSKSHIPSFTNEFWDNSAFNAHKTESEDEIMFSTSNGLDSQEADFGYQNLGLTSHLSLPSSSTKMTSMENFLEIQGSVPCKIRAKRGFATHPRSIAERVRRTRISDRIKKLQGLFPKSDKQTSTADMLDLAVEYIKDLQEQVQILTDCKEKCKCASHEKKPCKPCS*