Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013676.01.T01 | XP_003599875.1 | 93.939 | 297 | 15 | 1 | 1 | 297 | 1 | 294 | 0 | 545 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013676.01.T01 | G7IXZ8 | 93.939 | 297 | 15 | 1 | 1 | 297 | 1 | 294 | 0.0 | 545 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013676.01 | MsG0880046052.01 | -0.814374 | 1.591297e-51 | 1.065924e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013676.01.T01 | MTR_3g048300 | 93.939 | 297 | 15 | 1 | 1 | 297 | 1 | 294 | 0.0 | 545 |
| MsG0380013676.01.T01 | MTR_1g107225 | 31.405 | 121 | 71 | 3 | 177 | 296 | 105 | 214 | 1.13e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013676.01.T01 | AT2G42900 | 38.189 | 254 | 131 | 6 | 40 | 290 | 24 | 254 | 1.17e-50 | 169 |
| MsG0380013676.01.T01 | AT2G15130 | 25.794 | 252 | 152 | 8 | 48 | 296 | 4 | 223 | 1.31e-17 | 80.5 |
| MsG0380013676.01.T01 | AT2G15130 | 27.500 | 160 | 94 | 4 | 140 | 296 | 2 | 142 | 3.19e-13 | 66.2 |
Find 74 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATTATTGTTGTTGCTATT+AGG | 0.193254 | 3:-43167791 | None:intergenic |
| TATTATTGTTGTTGCTATTA+GGG | 0.216246 | 3:-43167790 | None:intergenic |
| TTGTAGCAATAATCTCAATA+TGG | 0.268337 | 3:+43167612 | MsG0380013676.01.T01:CDS |
| TGTATGATATTGTTGTTGTT+TGG | 0.297788 | 3:-43167569 | None:intergenic |
| GTATGATATTGTTGTTGTTT+GGG | 0.305696 | 3:-43167568 | None:intergenic |
| GTGAAGATGGACGTGAATTA+TGG | 0.353799 | 3:+43168137 | MsG0380013676.01.T01:CDS |
| ACGTTGAATTGCACCTACTA+TGG | 0.369600 | 3:-43167975 | None:intergenic |
| TATTGTGAAAAGTATGATAA+GGG | 0.380386 | 3:+43168202 | MsG0380013676.01.T01:CDS |
| AGAGACTCTCCGGTGGCGTC+GGG | 0.389400 | 3:+43168101 | MsG0380013676.01.T01:CDS |
| AACGTGCCATGGCTCGAGTT+TGG | 0.400973 | 3:+43167993 | MsG0380013676.01.T01:CDS |
| GACACAAAAGACTCCCTTGC+CGG | 0.408665 | 3:+43167680 | MsG0380013676.01.T01:CDS |
| CCATGGCTCGAGTTTGGCTA+TGG | 0.410462 | 3:+43167999 | MsG0380013676.01.T01:CDS |
| TTATTGCTACAAAGAGAATA+AGG | 0.414165 | 3:-43167601 | None:intergenic |
| TCTCAACACAAGCTCCTTTG+TGG | 0.420037 | 3:+43167754 | MsG0380013676.01.T01:CDS |
| GCTATGGGATGGAAGGTCTA+AGG | 0.423860 | 3:+43168015 | MsG0380013676.01.T01:CDS |
| TCACGTCCATCTTCACATTC+CGG | 0.428097 | 3:-43168130 | None:intergenic |
| GCACCACCTAAGCTTCTTGA+TGG | 0.436992 | 3:+43168037 | MsG0380013676.01.T01:CDS |
| TTGAAAGCCACGAAGCTTTG+TGG | 0.460187 | 3:+43168295 | MsG0380013676.01.T01:CDS |
| TGTAGCAATAATCTCAATAT+GGG | 0.460333 | 3:+43167613 | MsG0380013676.01.T01:CDS |
| GAATATATCGCCGAGTTAGC+CGG | 0.460919 | 3:+43168067 | MsG0380013676.01.T01:CDS |
| GAGAGACTCTCCGGTGGCGT+CGG | 0.467659 | 3:+43168100 | MsG0380013676.01.T01:CDS |
| GAGGTAGAAGTGGTTGGAAG+AGG | 0.469781 | 3:-43167472 | None:intergenic |
| GAGTCTCTCATGGTGGAATC+CGG | 0.472116 | 3:-43168086 | None:intergenic |
| CGGTGGCGTCGGGGAGTCGC+CGG | 0.473651 | 3:+43168111 | MsG0380013676.01.T01:CDS |
| TTATTGTGAAAAGTATGATA+AGG | 0.475643 | 3:+43168201 | MsG0380013676.01.T01:CDS |
| TGCTTCAAGATCAATGGAAA+AGG | 0.482871 | 3:+43167436 | None:intergenic |
| GTTTACTATGTGTATGTCAA+AGG | 0.496380 | 3:-43167657 | None:intergenic |
| TCGTATGGTGGGTGATGTGT+TGG | 0.499984 | 3:+43168270 | MsG0380013676.01.T01:CDS |
| ATACAATCCACAAAGCTTCG+TGG | 0.500886 | 3:-43168302 | None:intergenic |
| TGGTTGATCTCATAAGAAGA+AGG | 0.506415 | 3:-43167503 | None:intergenic |
| CGGCGACTCCCCGACGCCAC+CGG | 0.509632 | 3:-43168110 | None:intergenic |
| AGTAAAACGACGACCGGCAA+GGG | 0.510771 | 3:-43167693 | None:intergenic |
| CGTATGGTGGGTGATGTGTT+GGG | 0.510816 | 3:+43168271 | MsG0380013676.01.T01:CDS |
| TATGATATTGTTGTTGTTTG+GGG | 0.527832 | 3:-43167567 | None:intergenic |
| TTCAACAACATGGCCATAGT+AGG | 0.533722 | 3:+43167962 | MsG0380013676.01.T01:CDS |
| GAGTAAAACGACGACCGGCA+AGG | 0.534266 | 3:-43167694 | None:intergenic |
| ACCTAAGCTTCTTGATGGCA+TGG | 0.537344 | 3:+43168042 | MsG0380013676.01.T01:CDS |
| ACCATGCCATCAAGAAGCTT+AGG | 0.543404 | 3:-43168043 | None:intergenic |
| TTCCACCATGAGAGACTCTC+CGG | 0.544947 | 3:+43168091 | MsG0380013676.01.T01:CDS |
| TGAAATGAGGTAGAAGTGGT+TGG | 0.556787 | 3:-43167478 | None:intergenic |
| GAGTCGCCGGAATGTGAAGA+TGG | 0.560628 | 3:+43168124 | MsG0380013676.01.T01:CDS |
| GGGTTGAAATGAGGTAGAAG+TGG | 0.562274 | 3:-43167482 | None:intergenic |
| TTCTCATCTTCAAGTAACAT+AGG | 0.570391 | 3:-43167938 | None:intergenic |
| TCATAGCTTCATTCAACCGT+CGG | 0.576123 | 3:-43168231 | None:intergenic |
| CAATACCATCACTACGATCA+CGG | 0.578579 | 3:+43167874 | MsG0380013676.01.T01:CDS |
| GGTTGATCTCATAAGAAGAA+GGG | 0.579665 | 3:-43167502 | None:intergenic |
| AGGTGCAATTCAACGTGCCA+TGG | 0.581592 | 3:+43167982 | MsG0380013676.01.T01:CDS |
| ATGGTGGAATCCGGCTAACT+CGG | 0.584956 | 3:-43168077 | None:intergenic |
| TAAGAAGAAGGGTTGAAATG+AGG | 0.586070 | 3:-43167491 | None:intergenic |
| GAGTTTGGCTATGGGATGGA+AGG | 0.588588 | 3:+43168008 | MsG0380013676.01.T01:CDS |
| CATGGCTCGAGTTTGGCTAT+GGG | 0.589258 | 3:+43168000 | MsG0380013676.01.T01:CDS |
| ATCAGCCGTGATCGTAGTGA+TGG | 0.590578 | 3:-43167879 | None:intergenic |
| AACAAACGTGCAACATGAGT+AGG | 0.591262 | 3:-43168175 | None:intergenic |
| AGATACGTGGCATGATCGTA+TGG | 0.594739 | 3:+43168255 | MsG0380013676.01.T01:CDS |
| ACAAACGTGCAACATGAGTA+GGG | 0.594912 | 3:-43168174 | None:intergenic |
| GCTCGAGTTTGGCTATGGGA+TGG | 0.595627 | 3:+43168004 | MsG0380013676.01.T01:CDS |
| ATGATAAGGGGTTCATCCGA+CGG | 0.604749 | 3:+43168215 | MsG0380013676.01.T01:CDS |
| CGCCACCGGAGAGTCTCTCA+TGG | 0.612446 | 3:-43168096 | None:intergenic |
| ATTGTGAAAAGTATGATAAG+GGG | 0.613946 | 3:+43168203 | MsG0380013676.01.T01:CDS |
| GAGACTCTCCGGTGGCGTCG+GGG | 0.614043 | 3:+43168102 | MsG0380013676.01.T01:CDS |
| CCATAGCCAAACTCGAGCCA+TGG | 0.614429 | 3:-43167999 | None:intergenic |
| AGATGAGAATTTCAACAACA+TGG | 0.618288 | 3:+43167952 | MsG0380013676.01.T01:CDS |
| AAGATGGACGTGAATTATGG+TGG | 0.623253 | 3:+43168140 | MsG0380013676.01.T01:CDS |
| ATGCCATCAAGAAGCTTAGG+TGG | 0.626372 | 3:-43168040 | None:intergenic |
| AGATGGACGTGAATTATGGT+GGG | 0.644798 | 3:+43168141 | MsG0380013676.01.T01:CDS |
| ACGTGGCATGATCGTATGGT+GGG | 0.649763 | 3:+43168259 | MsG0380013676.01.T01:CDS |
| GTATAGAATTTGTTCCACAA+AGG | 0.669697 | 3:-43167768 | None:intergenic |
| TACGTGGCATGATCGTATGG+TGG | 0.675287 | 3:+43168258 | MsG0380013676.01.T01:CDS |
| TAAGCTTCTTGATGGCATGG+TGG | 0.679433 | 3:+43168045 | MsG0380013676.01.T01:CDS |
| TGTGTTGAGAAGTATACGTG+AGG | 0.686386 | 3:-43167741 | None:intergenic |
| TTGCTACAAAGAGAATAAGG+AGG | 0.702682 | 3:-43167598 | None:intergenic |
| CACCATGAGAGACTCTCCGG+TGG | 0.704399 | 3:+43168094 | MsG0380013676.01.T01:CDS |
| CACCGGAGAGTCTCTCATGG+TGG | 0.709405 | 3:-43168093 | None:intergenic |
| ATGAAGCTATGAAAGATACG+TGG | 0.742605 | 3:+43168242 | MsG0380013676.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTATTGTTGTTGCTATT+AGG | - | Chr3:43167794-43167813 | None:intergenic | 20.0% |
| !!! | TATTATTGTTGTTGCTATTA+GGG | - | Chr3:43167793-43167812 | None:intergenic | 20.0% |
| !!! | TATTGTGAAAAGTATGATAA+GGG | + | Chr3:43168202-43168221 | MsG0380013676.01.T01:CDS | 20.0% |
| !!! | TTATTGTGAAAAGTATGATA+AGG | + | Chr3:43168201-43168220 | MsG0380013676.01.T01:CDS | 20.0% |
| ! | TGTAGCAATAATCTCAATAT+GGG | + | Chr3:43167613-43167632 | MsG0380013676.01.T01:CDS | 25.0% |
| ! | TTATTGCTACAAAGAGAATA+AGG | - | Chr3:43167604-43167623 | None:intergenic | 25.0% |
| ! | TTGTAGCAATAATCTCAATA+TGG | + | Chr3:43167612-43167631 | MsG0380013676.01.T01:CDS | 25.0% |
| !! | ATTGTGAAAAGTATGATAAG+GGG | + | Chr3:43168203-43168222 | MsG0380013676.01.T01:CDS | 25.0% |
| !! | TGTATGATATTGTTGTTGTT+TGG | - | Chr3:43167572-43167591 | None:intergenic | 25.0% |
| !!! | GGTGAAATTTTTTGAATTGT+TGG | - | Chr3:43167549-43167568 | None:intergenic | 25.0% |
| !!! | GTATGATATTGTTGTTGTTT+GGG | - | Chr3:43167571-43167590 | None:intergenic | 25.0% |
| !!! | TATGATATTGTTGTTGTTTG+GGG | - | Chr3:43167570-43167589 | None:intergenic | 25.0% |
| AGATGAGAATTTCAACAACA+TGG | + | Chr3:43167952-43167971 | MsG0380013676.01.T01:CDS | 30.0% | |
| GTTTACTATGTGTATGTCAA+AGG | - | Chr3:43167660-43167679 | None:intergenic | 30.0% | |
| TTCTCATCTTCAAGTAACAT+AGG | - | Chr3:43167941-43167960 | None:intergenic | 30.0% | |
| ! | GTATAGAATTTGTTCCACAA+AGG | - | Chr3:43167771-43167790 | None:intergenic | 30.0% |
| ! | TTGTGTTGTAATCGTTTTGT+TGG | - | Chr3:43167526-43167545 | None:intergenic | 30.0% |
| AAAAAGAGTAAAACGACGAC+CGG | - | Chr3:43167702-43167721 | None:intergenic | 35.0% | |
| ATGAAGCTATGAAAGATACG+TGG | + | Chr3:43168242-43168261 | MsG0380013676.01.T01:CDS | 35.0% | |
| GGTTGATCTCATAAGAAGAA+GGG | - | Chr3:43167505-43167524 | None:intergenic | 35.0% | |
| TAAGAAGAAGGGTTGAAATG+AGG | - | Chr3:43167494-43167513 | None:intergenic | 35.0% | |
| TGGTTGATCTCATAAGAAGA+AGG | - | Chr3:43167506-43167525 | None:intergenic | 35.0% | |
| TTGCTACAAAGAGAATAAGG+AGG | - | Chr3:43167601-43167620 | None:intergenic | 35.0% | |
| AACAAACGTGCAACATGAGT+AGG | - | Chr3:43168178-43168197 | None:intergenic | 40.0% | |
| AAGATGGACGTGAATTATGG+TGG | + | Chr3:43168140-43168159 | MsG0380013676.01.T01:CDS | 40.0% | |
| ACAAACGTGCAACATGAGTA+GGG | - | Chr3:43168177-43168196 | None:intergenic | 40.0% | |
| ACGTTGAATTGCACCTACTA+TGG | - | Chr3:43167978-43167997 | None:intergenic | 40.0% | |
| AGATGGACGTGAATTATGGT+GGG | + | Chr3:43168141-43168160 | MsG0380013676.01.T01:CDS | 40.0% | |
| ATACAATCCACAAAGCTTCG+TGG | - | Chr3:43168305-43168324 | None:intergenic | 40.0% | |
| CAATACCATCACTACGATCA+CGG | + | Chr3:43167874-43167893 | MsG0380013676.01.T01:CDS | 40.0% | |
| GTGAAGATGGACGTGAATTA+TGG | + | Chr3:43168137-43168156 | MsG0380013676.01.T01:CDS | 40.0% | |
| TCATAGCTTCATTCAACCGT+CGG | - | Chr3:43168234-43168253 | None:intergenic | 40.0% | |
| TGAAATGAGGTAGAAGTGGT+TGG | - | Chr3:43167481-43167500 | None:intergenic | 40.0% | |
| TTCAACAACATGGCCATAGT+AGG | + | Chr3:43167962-43167981 | MsG0380013676.01.T01:CDS | 40.0% | |
| ! | TGTGTTGAGAAGTATACGTG+AGG | - | Chr3:43167744-43167763 | None:intergenic | 40.0% |
| ACCATGCCATCAAGAAGCTT+AGG | - | Chr3:43168046-43168065 | None:intergenic | 45.0% | |
| AGATACGTGGCATGATCGTA+TGG | + | Chr3:43168255-43168274 | MsG0380013676.01.T01:CDS | 45.0% | |
| ATGATAAGGGGTTCATCCGA+CGG | + | Chr3:43168215-43168234 | MsG0380013676.01.T01:CDS | 45.0% | |
| ATGCCATCAAGAAGCTTAGG+TGG | - | Chr3:43168043-43168062 | None:intergenic | 45.0% | |
| GAATATATCGCCGAGTTAGC+CGG | + | Chr3:43168067-43168086 | MsG0380013676.01.T01:CDS | 45.0% | |
| GGGTTGAAATGAGGTAGAAG+TGG | - | Chr3:43167485-43167504 | None:intergenic | 45.0% | |
| TCACGTCCATCTTCACATTC+CGG | - | Chr3:43168133-43168152 | None:intergenic | 45.0% | |
| TCTCAACACAAGCTCCTTTG+TGG | + | Chr3:43167754-43167773 | MsG0380013676.01.T01:CDS | 45.0% | |
| TTGAAAGCCACGAAGCTTTG+TGG | + | Chr3:43168295-43168314 | MsG0380013676.01.T01:CDS | 45.0% | |
| ! | ACCTAAGCTTCTTGATGGCA+TGG | + | Chr3:43168042-43168061 | MsG0380013676.01.T01:CDS | 45.0% |
| ! | TAAGCTTCTTGATGGCATGG+TGG | + | Chr3:43168045-43168064 | MsG0380013676.01.T01:CDS | 45.0% |
| ACGTGGCATGATCGTATGGT+GGG | + | Chr3:43168259-43168278 | MsG0380013676.01.T01:CDS | 50.0% | |
| AGGTGCAATTCAACGTGCCA+TGG | + | Chr3:43167982-43168001 | MsG0380013676.01.T01:CDS | 50.0% | |
| AGTAAAACGACGACCGGCAA+GGG | - | Chr3:43167696-43167715 | None:intergenic | 50.0% | |
| ATCAGCCGTGATCGTAGTGA+TGG | - | Chr3:43167882-43167901 | None:intergenic | 50.0% | |
| CATGGCTCGAGTTTGGCTAT+GGG | + | Chr3:43168000-43168019 | MsG0380013676.01.T01:CDS | 50.0% | |
| GACACAAAAGACTCCCTTGC+CGG | + | Chr3:43167680-43167699 | MsG0380013676.01.T01:CDS | 50.0% | |
| GAGGTAGAAGTGGTTGGAAG+AGG | - | Chr3:43167475-43167494 | None:intergenic | 50.0% | |
| GAGTTTGGCTATGGGATGGA+AGG | + | Chr3:43168008-43168027 | MsG0380013676.01.T01:CDS | 50.0% | |
| GCACCACCTAAGCTTCTTGA+TGG | + | Chr3:43168037-43168056 | MsG0380013676.01.T01:CDS | 50.0% | |
| GCTATGGGATGGAAGGTCTA+AGG | + | Chr3:43168015-43168034 | MsG0380013676.01.T01:CDS | 50.0% | |
| TACGTGGCATGATCGTATGG+TGG | + | Chr3:43168258-43168277 | MsG0380013676.01.T01:CDS | 50.0% | |
| TTCCACCATGAGAGACTCTC+CGG | + | Chr3:43168091-43168110 | MsG0380013676.01.T01:CDS | 50.0% | |
| ! | ATGGTGGAATCCGGCTAACT+CGG | - | Chr3:43168080-43168099 | None:intergenic | 50.0% |
| ! | GAGTCTCTCATGGTGGAATC+CGG | - | Chr3:43168089-43168108 | None:intergenic | 50.0% |
| !! | CGTATGGTGGGTGATGTGTT+GGG | + | Chr3:43168271-43168290 | MsG0380013676.01.T01:CDS | 50.0% |
| !! | TCGTATGGTGGGTGATGTGT+TGG | + | Chr3:43168270-43168289 | MsG0380013676.01.T01:CDS | 50.0% |
| AACGTGCCATGGCTCGAGTT+TGG | + | Chr3:43167993-43168012 | MsG0380013676.01.T01:CDS | 55.0% | |
| CCATAGCCAAACTCGAGCCA+TGG | - | Chr3:43168002-43168021 | None:intergenic | 55.0% | |
| CCATGGCTCGAGTTTGGCTA+TGG | + | Chr3:43167999-43168018 | MsG0380013676.01.T01:CDS | 55.0% | |
| GAGTAAAACGACGACCGGCA+AGG | - | Chr3:43167697-43167716 | None:intergenic | 55.0% | |
| GAGTCGCCGGAATGTGAAGA+TGG | + | Chr3:43168124-43168143 | MsG0380013676.01.T01:CDS | 55.0% | |
| GCTCGAGTTTGGCTATGGGA+TGG | + | Chr3:43168004-43168023 | MsG0380013676.01.T01:CDS | 55.0% | |
| CACCATGAGAGACTCTCCGG+TGG | + | Chr3:43168094-43168113 | MsG0380013676.01.T01:CDS | 60.0% | |
| CACCGGAGAGTCTCTCATGG+TGG | - | Chr3:43168096-43168115 | None:intergenic | 60.0% | |
| AGAGACTCTCCGGTGGCGTC+GGG | + | Chr3:43168101-43168120 | MsG0380013676.01.T01:CDS | 65.0% | |
| CGCCACCGGAGAGTCTCTCA+TGG | - | Chr3:43168099-43168118 | None:intergenic | 65.0% | |
| GAGAGACTCTCCGGTGGCGT+CGG | + | Chr3:43168100-43168119 | MsG0380013676.01.T01:CDS | 65.0% | |
| GAGACTCTCCGGTGGCGTCG+GGG | + | Chr3:43168102-43168121 | MsG0380013676.01.T01:CDS | 70.0% | |
| ! | CGGCGACTCCCCGACGCCAC+CGG | - | Chr3:43168113-43168132 | None:intergenic | 80.0% |
| !! | CGGTGGCGTCGGGGAGTCGC+CGG | + | Chr3:43168111-43168130 | MsG0380013676.01.T01:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 43167449 | 43168342 | 43167449 | ID=MsG0380013676.01;Name=MsG0380013676.01 |
| Chr3 | mRNA | 43167449 | 43168342 | 43167449 | ID=MsG0380013676.01.T01;Parent=MsG0380013676.01;Name=MsG0380013676.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|297 |
| Chr3 | exon | 43167449 | 43168342 | 43167449 | ID=MsG0380013676.01.T01:exon:4377;Parent=MsG0380013676.01.T01 |
| Chr3 | CDS | 43167449 | 43168342 | 43167449 | ID=MsG0380013676.01.T01:cds;Parent=MsG0380013676.01.T01 |
| Gene Sequence |
| Protein sequence |
