Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014015.01.T01 | RHN51228.1 | 98.592 | 71 | 1 | 0 | 5 | 75 | 294 | 364 | 2.07E-37 | 140 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014015.01.T01 | Q9TKI5 | 98.592 | 71 | 1 | 0 | 5 | 75 | 421 | 491 | 1.09E-39 | 140 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014015.01.T01 | A0A396I254 | 98.592 | 71 | 1 | 0 | 5 | 75 | 402 | 472 | 2.75e-37 | 140 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049078.01 | MsG0380014015.01 | 0.805897 | 1.069399e-49 | 5.724859e-47 |
| MsG0380013955.01 | MsG0380014015.01 | 0.804350 | 2.253156e-49 | 1.159115e-46 |
| MsG0380013970.01 | MsG0380014015.01 | 0.805474 | 1.311854e-49 | 6.946777e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014015.01.T01 | MTR_2g089590 | 90.909 | 88 | 8 | 0 | 5 | 92 | 13 | 100 | 4.00e-41 | 131 |
| MsG0380014015.01.T01 | MTR_3g035685 | 98.592 | 71 | 1 | 0 | 5 | 75 | 421 | 491 | 1.13e-40 | 140 |
| MsG0380014015.01.T01 | MTR_4g051280 | 100.000 | 67 | 0 | 0 | 9 | 75 | 407 | 473 | 7.25e-39 | 135 |
| MsG0380014015.01.T01 | MTR_7g021760 | 82.022 | 89 | 10 | 2 | 5 | 91 | 404 | 488 | 1.00e-28 | 108 |
| MsG0380014015.01.T01 | MTR_0002s0390 | 72.973 | 37 | 10 | 0 | 5 | 41 | 425 | 461 | 6.36e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014015.01.T01 | ATCG00040 | 71.831 | 71 | 19 | 1 | 5 | 75 | 442 | 511 | 4.28e-23 | 92.8 |
Find 10 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAGAAATCGGATTGGTATT+TGG | 0.177848 | 3:+48658743 | MsG0380014015.01.T01:CDS |
| ATCAGGTTCAGAAGAATTAT+TGG | 0.233020 | 3:+48658645 | MsG0380014015.01.T01:CDS |
| TGCACAGGTTAAATAGAAAT+CGG | 0.394621 | 3:+48658731 | MsG0380014015.01.T01:CDS |
| GCTTTCTTGTATTAAAACTT+TGG | 0.413392 | 3:+48658582 | MsG0380014015.01.T01:CDS |
| TGCAAAGTAGAAGAATCTCT+TGG | 0.487044 | 3:-48658712 | None:intergenic |
| GAGATTCTTCTACTTTGCAC+AGG | 0.513432 | 3:+48658716 | MsG0380014015.01.T01:CDS |
| ATCGAATAAAATATATACTT+CGG | 0.528810 | 3:+48658560 | MsG0380014015.01.T01:CDS |
| TCTGGTCAATGATGAATGAT+TGG | 0.537179 | 3:+48658785 | MsG0380014015.01.T01:CDS |
| ATTGGAAGAATTCTTTACAG+AGG | 0.616541 | 3:+48658663 | MsG0380014015.01.T01:CDS |
| AGGTTAAATAGAAATCGGAT+TGG | 0.652592 | 3:+48658736 | MsG0380014015.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TACAAATTTTTTTTTTTTTG+AGG | - | Chr3:48658541-48658560 | None:intergenic | 10.0% |
| !! | ATCGAATAAAATATATACTT+CGG | + | Chr3:48658560-48658579 | MsG0380014015.01.T01:CDS | 15.0% |
| ! | GCTTTCTTGTATTAAAACTT+TGG | + | Chr3:48658582-48658601 | MsG0380014015.01.T01:CDS | 25.0% |
| AGGTTAAATAGAAATCGGAT+TGG | + | Chr3:48658736-48658755 | MsG0380014015.01.T01:CDS | 30.0% | |
| ATCAGGTTCAGAAGAATTAT+TGG | + | Chr3:48658645-48658664 | MsG0380014015.01.T01:CDS | 30.0% | |
| ATTGGAAGAATTCTTTACAG+AGG | + | Chr3:48658663-48658682 | MsG0380014015.01.T01:CDS | 30.0% | |
| TGCACAGGTTAAATAGAAAT+CGG | + | Chr3:48658731-48658750 | MsG0380014015.01.T01:CDS | 30.0% | |
| !! | ATAGAAATCGGATTGGTATT+TGG | + | Chr3:48658743-48658762 | MsG0380014015.01.T01:CDS | 30.0% |
| TCTGGTCAATGATGAATGAT+TGG | + | Chr3:48658785-48658804 | MsG0380014015.01.T01:CDS | 35.0% | |
| TGCAAAGTAGAAGAATCTCT+TGG | - | Chr3:48658715-48658734 | None:intergenic | 35.0% | |
| ! | TATTCTTTTCAGCAACGATC+TGG | + | Chr3:48658767-48658786 | MsG0380014015.01.T01:CDS | 35.0% |
| GAGATTCTTCTACTTTGCAC+AGG | + | Chr3:48658716-48658735 | MsG0380014015.01.T01:CDS | 40.0% | |
| !!! | CGCGCTTTTTTGAAAAGATC+AGG | + | Chr3:48658628-48658647 | MsG0380014015.01.T01:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48658532 | 48658861 | 48658532 | ID=MsG0380014015.01;Name=MsG0380014015.01 |
| Chr3 | mRNA | 48658532 | 48658861 | 48658532 | ID=MsG0380014015.01.T01;Parent=MsG0380014015.01;Name=MsG0380014015.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|109 |
| Chr3 | exon | 48658532 | 48658861 | 48658532 | ID=MsG0380014015.01.T01:exon:8571;Parent=MsG0380014015.01.T01 |
| Chr3 | CDS | 48658532 | 48658861 | 48658532 | ID=MsG0380014015.01.T01:cds;Parent=MsG0380014015.01.T01 |
| Gene Sequence |
| Protein sequence |
