Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014574.01.T01 | XP_003600390.2 | 98.551 | 69 | 1 | 0 | 1 | 69 | 281 | 349 | 8.80E-41 | 146 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014574.01.T01 | G7IXR9 | 98.551 | 69 | 1 | 0 | 1 | 69 | 281 | 349 | 4.20e-41 | 146 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011293.01 | MsG0380014574.01 | 0.804085 | 2.558316e-49 | 1.307179e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014574.01.T01 | MTR_3g060670 | 98.551 | 69 | 1 | 0 | 1 | 69 | 281 | 349 | 1.07e-44 | 146 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGTAGAGTTTCACAAAT+TGG | 0.219579 | 3:-56653034 | MsG0380014574.01.T01:CDS |
| AGCTGGTAAATCCATTATTC+TGG | 0.244577 | 3:+56653007 | None:intergenic |
| AGAAATGGTCTTGCAGTTAA+TGG | 0.260564 | 3:-56652982 | MsG0380014574.01.T01:CDS |
| AAATTGGAAATCCAGAATAA+TGG | 0.364472 | 3:-56653018 | MsG0380014574.01.T01:CDS |
| TTTGTGAAACTCTACTTCAA+TGG | 0.429290 | 3:+56653038 | None:intergenic |
| GCATCATCAGGGTCTGGCTC+TGG | 0.452433 | 3:+56653120 | None:intergenic |
| AAAGATCCTTGTCTCATCAT+TGG | 0.469541 | 3:+56653156 | None:intergenic |
| TGCAAGACCATTTCTTGAGC+TGG | 0.476422 | 3:+56652990 | None:intergenic |
| GGATTTACCAGCTCAAGAAA+TGG | 0.518533 | 3:-56652997 | MsG0380014574.01.T01:CDS |
| AAATTGGAAAATCAGAATAA+CGG | 0.540654 | 3:-56653063 | MsG0380014574.01.T01:CDS |
| ATGTCACCAATGATGAGACA+AGG | 0.672091 | 3:-56653162 | MsG0380014574.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGGAAAATCAGAATAA+CGG | - | Chr3:56653074-56653093 | MsG0380014574.01.T01:CDS | 20.0% |
| ! | AAATTGGAAATCCAGAATAA+TGG | - | Chr3:56653119-56653138 | MsG0380014574.01.T01:CDS | 25.0% |
| ! | TGAAGTTGAGTTTCAAAAAT+TGG | - | Chr3:56653058-56653077 | MsG0380014574.01.T01:CDS | 25.0% |
| !!! | TTTTTGAAACTCAACTTCAA+TGG | + | Chr3:56653057-56653076 | None:intergenic | 25.0% |
| TGAAGTAGAGTTTCACAAAT+TGG | - | Chr3:56653103-56653122 | MsG0380014574.01.T01:CDS | 30.0% | |
| TTTGTGAAACTCTACTTCAA+TGG | + | Chr3:56653102-56653121 | None:intergenic | 30.0% | |
| ! | TTCAATGGTTTTCGCTATTT+TGG | + | Chr3:56653042-56653061 | None:intergenic | 30.0% |
| AAAGATCCTTGTCTCATCAT+TGG | + | Chr3:56652984-56653003 | None:intergenic | 35.0% | |
| AGCTGGTAAATCCATTATTC+TGG | + | Chr3:56653133-56653152 | None:intergenic | 35.0% | |
| ! | AGAAATGGTCTTGCAGTTAA+TGG | - | Chr3:56653155-56653174 | MsG0380014574.01.T01:CDS | 35.0% |
| ATGTCACCAATGATGAGACA+AGG | - | Chr3:56652975-56652994 | MsG0380014574.01.T01:CDS | 40.0% | |
| GGATTTACCAGCTCAAGAAA+TGG | - | Chr3:56653140-56653159 | MsG0380014574.01.T01:CDS | 40.0% | |
| !!! | TCGCTATTTTGGCATCATCA+GGG | + | Chr3:56653031-56653050 | None:intergenic | 40.0% |
| !!! | TTCGCTATTTTGGCATCATC+AGG | + | Chr3:56653032-56653051 | None:intergenic | 40.0% |
| TGCAAGACCATTTCTTGAGC+TGG | + | Chr3:56653150-56653169 | None:intergenic | 45.0% | |
| !! | ATTTTGGCATCATCAGGGTC+TGG | + | Chr3:56653026-56653045 | None:intergenic | 45.0% |
| ! | GCATCATCAGGGTCTGGCTC+TGG | + | Chr3:56653020-56653039 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 56652975 | 56653184 | 56652975 | ID=MsG0380014574.01;Name=MsG0380014574.01 |
| Chr3 | mRNA | 56652975 | 56653184 | 56652975 | ID=MsG0380014574.01.T01;Parent=MsG0380014574.01;Name=MsG0380014574.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|69 |
| Chr3 | exon | 56652975 | 56653184 | 56652975 | ID=MsG0380014574.01.T01:exon:7491;Parent=MsG0380014574.01.T01 |
| Chr3 | CDS | 56652975 | 56653184 | 56652975 | ID=MsG0380014574.01.T01:cds;Parent=MsG0380014574.01.T01 |
| Gene Sequence |
| Protein sequence |
