Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015618.01.T01 | XP_039687382.1 | 97.311 | 595 | 14 | 1 | 14 | 608 | 1 | 593 | 0 | 1197 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015618.01.T01 | MTR_3g073420 | 97.311 | 595 | 14 | 1 | 14 | 608 | 1 | 593 | 0.0 | 1197 |
| MsG0380015618.01.T01 | MTR_5g061220 | 44.846 | 553 | 250 | 14 | 32 | 573 | 19 | 527 | 6.93e-146 | 432 |
| MsG0380015618.01.T01 | MTR_2g094570 | 43.182 | 572 | 289 | 14 | 17 | 569 | 52 | 606 | 2.00e-129 | 398 |
| MsG0380015618.01.T01 | MTR_2g094570 | 43.494 | 561 | 281 | 14 | 28 | 569 | 7 | 550 | 2.25e-129 | 395 |
| MsG0380015618.01.T01 | MTR_2g094570 | 43.494 | 561 | 281 | 14 | 28 | 569 | 7 | 550 | 3.36e-129 | 395 |
| MsG0380015618.01.T01 | MTR_5g040880 | 40.598 | 569 | 261 | 14 | 29 | 577 | 8 | 519 | 9.37e-128 | 386 |
| MsG0380015618.01.T01 | MTR_2g024430 | 40.642 | 561 | 267 | 12 | 22 | 572 | 10 | 514 | 1.77e-127 | 385 |
| MsG0380015618.01.T01 | MTR_4g058930 | 47.991 | 448 | 215 | 10 | 29 | 463 | 9 | 451 | 1.58e-124 | 382 |
| MsG0380015618.01.T01 | MTR_4g058930 | 47.991 | 448 | 215 | 10 | 29 | 463 | 9 | 451 | 3.87e-124 | 382 |
| MsG0380015618.01.T01 | MTR_7g101280 | 42.857 | 525 | 249 | 16 | 28 | 516 | 27 | 536 | 4.51e-124 | 380 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015618.01.T01 | AT1G77850 | 47.950 | 561 | 269 | 11 | 25 | 575 | 12 | 559 | 4.08e-164 | 482 |
| MsG0380015618.01.T01 | AT1G77850 | 47.950 | 561 | 269 | 11 | 25 | 575 | 12 | 559 | 4.18e-164 | 481 |
| MsG0380015618.01.T01 | AT4G30080 | 42.376 | 564 | 284 | 14 | 29 | 574 | 16 | 556 | 1.17e-121 | 375 |
| MsG0380015618.01.T01 | AT2G28350 | 46.389 | 457 | 209 | 10 | 28 | 460 | 6 | 450 | 1.03e-119 | 370 |
| MsG0380015618.01.T01 | AT2G28350 | 46.389 | 457 | 209 | 10 | 28 | 460 | 6 | 450 | 1.99e-119 | 370 |
| MsG0380015618.01.T01 | AT5G37020 | 37.101 | 407 | 211 | 8 | 29 | 426 | 19 | 389 | 1.04e-69 | 240 |
| MsG0380015618.01.T01 | AT5G37020 | 37.101 | 407 | 211 | 8 | 29 | 426 | 19 | 389 | 2.86e-69 | 240 |
| MsG0380015618.01.T01 | AT1G30330 | 38.366 | 404 | 201 | 8 | 21 | 412 | 10 | 377 | 1.10e-68 | 240 |
| MsG0380015618.01.T01 | AT1G30330 | 38.366 | 404 | 201 | 8 | 21 | 412 | 10 | 377 | 1.10e-68 | 240 |
| MsG0380015618.01.T01 | AT1G30330 | 38.250 | 400 | 203 | 7 | 21 | 412 | 12 | 375 | 1.47e-68 | 240 |
Find 222 sgRNAs with CRISPR-Local
Find 332 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGATGGTCCTGTCTGCA+TGG | 0.562967 | 3:-72601142 | None:intergenic |
| AAAAGGACTAGAGGGGTATC+TGG | 0.298766 | 3:+72599743 | MsG0380015618.01.T01:CDS |
| AAAAGTAAATGAAAATGCAG+AGG | 0.617273 | 3:+72600753 | MsG0380015618.01.T01:three_prime_UTR |
| AAACCCTAGAATTGACGGAG+GGG | 0.688106 | 3:-72597683 | None:intergenic |
| AAACTCTGGCGAGCTATCGC+CGG | 0.546085 | 3:+72597645 | MsG0380015618.01.T01:CDS |
| AAAGAAAACAGCATACTGCA+AGG | 0.660360 | 3:-72601167 | None:intergenic |
| AAAGAAGTTCCACCTGCCAA+GGG | 0.653496 | 3:-72599682 | None:intergenic |
| AAAGGACTAGAGGGGTATCT+GGG | 0.471476 | 3:+72599744 | MsG0380015618.01.T01:CDS |
| AAAGTAAATGAAAATGCAGA+GGG | 0.606963 | 3:+72600754 | MsG0380015618.01.T01:three_prime_UTR |
| AAAGTACTGTAACTCAACAA+TGG | 0.650302 | 3:-72599842 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACCAATGGATACCATTAT+TGG | + | Chr3:72599235-72599254 | MsG0380015618.01.T01:intron | 30.0% | |
| AACCAATGGATACCATTATT+GGG | + | Chr3:72599236-72599255 | MsG0380015618.01.T01:intron | 30.0% | |
| AATCTCATAAGTCCCAATAA+TGG | - | Chr3:72599251-72599270 | None:intergenic | 30.0% | |
| AGACATAAGAAATCAAAAGC+AGG | - | Chr3:72599484-72599503 | None:intergenic | 30.0% | |
| AGTGAATCAAAGTTCTCAAT+AGG | + | Chr3:72600394-72600413 | MsG0380015618.01.T01:three_prime_UTR | 30.0% | |
| ATACTTAGTTTCATTGTTCC+CGG | - | Chr3:72601210-72601229 | None:intergenic | 30.0% | |
| ATGAGTATTCTCATGTGAAT+AGG | + | Chr3:72600830-72600849 | MsG0380015618.01.T01:three_prime_UTR | 30.0% | |
| ATTTCCTAAAGTACAATGGT+TGG | + | Chr3:72601278-72601297 | MsG0380015618.01.T01:three_prime_UTR | 30.0% | |
| CATTGTTCAGAACTTTATTG+GGG | + | Chr3:72600906-72600925 | MsG0380015618.01.T01:three_prime_UTR | 30.0% | |
| GCAATCCAGATTTCAAAAAA+AGG | - | Chr3:72599334-72599353 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 72597469 | 72601302 | 72597469 | ID=MsG0380015618.01;Name=MsG0380015618.01 |
| Chr3 | mRNA | 72597469 | 72601302 | 72597469 | ID=MsG0380015618.01.T01;Parent=MsG0380015618.01;Name=MsG0380015618.01.T01;_AED=0.00;_eAED=0.00;_QI=83|1|1|1|0.5|0.33|3|836|608 |
| Chr3 | exon | 72597469 | 72598673 | 72597469 | ID=MsG0380015618.01.T01:exon:14035;Parent=MsG0380015618.01.T01 |
| Chr3 | exon | 72599635 | 72601006 | 72599635 | ID=MsG0380015618.01.T01:exon:14036;Parent=MsG0380015618.01.T01 |
| Chr3 | exon | 72601134 | 72601302 | 72601134 | ID=MsG0380015618.01.T01:exon:14037;Parent=MsG0380015618.01.T01 |
| Chr3 | five_prime_UTR | 72597469 | 72597551 | 72597469 | ID=MsG0380015618.01.T01:five_prime_utr;Parent=MsG0380015618.01.T01 |
| Chr3 | CDS | 72597552 | 72598673 | 72597552 | ID=MsG0380015618.01.T01:cds;Parent=MsG0380015618.01.T01 |
| Chr3 | CDS | 72599635 | 72600339 | 72599635 | ID=MsG0380015618.01.T01:cds;Parent=MsG0380015618.01.T01 |
| Chr3 | three_prime_UTR | 72600340 | 72601006 | 72600340 | ID=MsG0380015618.01.T01:three_prime_utr;Parent=MsG0380015618.01.T01 |
| Chr3 | three_prime_UTR | 72601134 | 72601302 | 72601134 | ID=MsG0380015618.01.T01:three_prime_utr;Parent=MsG0380015618.01.T01 |
| Gene Sequence |
>MsG0380015618.01.T01 ATGTTCGAAAAACCCTCACTCATTTTCATTCAACCAATCATGAACCGCCAAGTACTACCACCGTCTCCAAAAAACGCAACCACACTCAACCCCAAACTCTGGCGAGCTATCGCCGGCGCTGCCGTGCAAATCCCCTCCGTCAATTCTAGGGTTTACTACTTCCCTCAAGGTCACATGGACCAAGCTACTTCACTTCCGAACAATCTCTCACCTCTTCTTCTCTCTCGTCCCTACATTCTCTGCTCCGTCTCCGCCGTTCATTTCCTCGCCGATCCGAAAACCGATGAGGTTTTCGCTAAGCTTTTTCTTCAACCGTTGAATGATTTCACTGTTAATTTTCCACGGATTCCGGTGATTGAAGCTGACGACGGTGAGAGGATTTCTTCGTTTGCGAAGATTCTTACTCCTTCTGATGCGAACAACGGTGGTGGATTTTCTGTTCCGAGGTTTTGTGCTGATTCGATTTTTCCTCCGTTGGATTATAGTATGGATCCTCCGTTGCAGAATTTGTTGATTACTGATGTTCATGGTTTGACTTGGGAGTTTCGTCACATTTACCGTGGAACGCCGCGCCGGCATTTGTTGACCACCGGTTGGAGTAGGTTTGTGAACGCTAAGAAGCTTGTTGCTGGTGATTCCGTTGTTTTTATGAAGAATACTAGAGGTGCAATGTTCATCGGAATCCGTCGTGCTGTTCGATTAGTTCCGAATCGGAGTAGCGCCGGTGGCGGTGTTTGCTCCGATGTGTCGAGATTGTGTCTTCCGATCTGTGGAGTGAGAAGTAGGGTGGATGAAGAAGAGAAGCTGGTGGATGAGAAGGCGTTTTCGAGGCACGGGAAGGGGAAGTTGTCTCCGGTGGCGGTTGCGGAGGCGGCGGAAATGGCAGCACAGGGGATGGGGTTTGAAGTGGTGTATTATCCGAGAGCGGGGTGGTCGGATTTTGTGTTGAAAGCGGAGGTTGTGGATGCGGCGATGAGTGTTACTTGGTGTCCTGGAATGAGAATCAAAATGGCTGTGGAGACTGATGATTCTTCCAGGACTACTTGGTTTCAAGGTGTAGTATCTCAGGTTTCTGTTCCTGATCGTGGGGCTTGGAGAGGTTCACCTTGGCGTATGCTTCATATTACATGGGACGAACCTGAAGTGTTGCAAACTTCCAAGTGGGTCAGCCCTTGGCAGGTGGAACTTCTTTCTACTACACCTTCCCTTCATACGCCCTTTCCCCCATTGAAAAGGACTAGAGGGGTATCTGGGGTGTTAACTGATGGAGATGGAGACCCCTTTTCTATCACAGGGTTCACTAATTCAACAATGGGACAATTGAATCAACCATTGTTGAGTTACAGTACTTTTCCTGCTGGCATGCAGGGAGCCAGGCATGATCTATTTTCCGTTTCAAGTTTTTCCAACTTTCCAGGTGATAACTTCCGTCTTTGTATGGGTAACTCCTTTGGCAGCAACACAGTGCCGGGGTTAAAATCTTTTTCAACGGATCTAAACGTTGGCAGTTCTCACTCTGGTGATTTTTCACCAGAGAGCCAGAGTAGTTTGCCTTCCTTTGGCACAGATTTTGTTCGGAACTACAACTGCAACTCAATGAAACCCGGGCCTGTAACGTTTCAGCTATTTGGTGCAGTCATTCAGACAGAACAGCCCGTTGAAAGTGGTTCAAATGGTACTGTTAGCACAGGAGATGACAGCAGTAAGGGCTGCAACGAAACTGAAGGCATTAACAACCCTCTTGAGGATTCGACTTACTCAAAATTGCTCGACAGGCTTGATGGCCAGTGCCAAATAGCCTCTACAGTTGAGGCATGCTATCTGTGA |
| Protein sequence |
>MsG0380015618.01.T01 MFEKPSLIFIQPIMNRQVLPPSPKNATTLNPKLWRAIAGAAVQIPSVNSRVYYFPQGHMDQATSLPNNLSPLLLSRPYILCSVSAVHFLADPKTDEVFAKLFLQPLNDFTVNFPRIPVIEADDGERISSFAKILTPSDANNGGGFSVPRFCADSIFPPLDYSMDPPLQNLLITDVHGLTWEFRHIYRGTPRRHLLTTGWSRFVNAKKLVAGDSVVFMKNTRGAMFIGIRRAVRLVPNRSSAGGGVCSDVSRLCLPICGVRSRVDEEEKLVDEKAFSRHGKGKLSPVAVAEAAEMAAQGMGFEVVYYPRAGWSDFVLKAEVVDAAMSVTWCPGMRIKMAVETDDSSRTTWFQGVVSQVSVPDRGAWRGSPWRMLHITWDEPEVLQTSKWVSPWQVELLSTTPSLHTPFPPLKRTRGVSGVLTDGDGDPFSITGFTNSTMGQLNQPLLSYSTFPAGMQGARHDLFSVSSFSNFPGDNFRLCMGNSFGSNTVPGLKSFSTDLNVGSSHSGDFSPESQSSLPSFGTDFVRNYNCNSMKPGPVTFQLFGAVIQTEQPVESGSNGTVSTGDDSSKGCNETEGINNPLEDSTYSKLLDRLDGQCQIASTVEACYL* |
