Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015749.01.T01 | XP_003601156.1 | 98.113 | 212 | 2 | 1 | 1 | 210 | 1 | 212 | 5.50E-150 | 430 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015749.01.T01 | G7J482 | 98.113 | 212 | 2 | 1 | 1 | 210 | 1 | 212 | 2.63e-150 | 430 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280009241.01 | MsG0380015749.01 | 0.800368 | 1.489001e-48 | 6.921187e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015749.01.T01 | MTR_3g076610 | 98.113 | 212 | 2 | 1 | 1 | 210 | 1 | 212 | 6.66e-154 | 430 |
| MsG0380015749.01.T01 | MTR_4g104760 | 78.774 | 212 | 34 | 4 | 1 | 208 | 1 | 205 | 5.78e-120 | 343 |
| MsG0380015749.01.T01 | MTR_5g069570 | 52.147 | 163 | 78 | 0 | 35 | 197 | 40 | 202 | 1.52e-59 | 189 |
| MsG0380015749.01.T01 | MTR_3g082430 | 43.931 | 173 | 95 | 2 | 26 | 196 | 13 | 185 | 3.55e-49 | 162 |
| MsG0380015749.01.T01 | MTR_5g069550 | 48.611 | 144 | 60 | 1 | 36 | 179 | 41 | 170 | 1.19e-43 | 145 |
| MsG0380015749.01.T01 | MTR_1g081260 | 36.082 | 194 | 118 | 2 | 7 | 196 | 4 | 195 | 3.40e-42 | 145 |
| MsG0380015749.01.T01 | MTR_7g095100 | 38.191 | 199 | 112 | 3 | 7 | 196 | 4 | 200 | 6.48e-41 | 141 |
| MsG0380015749.01.T01 | MTR_5g096570 | 44.025 | 159 | 89 | 0 | 35 | 193 | 1196 | 1354 | 2.04e-40 | 147 |
| MsG0380015749.01.T01 | MTR_7g114790 | 37.696 | 191 | 105 | 4 | 7 | 189 | 4 | 188 | 3.28e-37 | 132 |
| MsG0380015749.01.T01 | MTR_2g084965 | 34.524 | 168 | 102 | 5 | 34 | 193 | 46 | 213 | 8.49e-24 | 96.7 |
| MsG0380015749.01.T01 | MTR_4g055230 | 35.119 | 168 | 102 | 5 | 34 | 196 | 44 | 209 | 7.28e-23 | 94.4 |
| MsG0380015749.01.T01 | MTR_3g106250 | 27.317 | 205 | 124 | 7 | 7 | 195 | 6 | 201 | 8.56e-20 | 86.7 |
| MsG0380015749.01.T01 | MTR_1g013100 | 29.756 | 205 | 118 | 10 | 7 | 198 | 8 | 199 | 2.96e-18 | 82.0 |
| MsG0380015749.01.T01 | MTR_7g103900 | 30.822 | 146 | 99 | 2 | 46 | 189 | 56 | 201 | 3.35e-17 | 77.8 |
| MsG0380015749.01.T01 | MTR_7g103900 | 30.822 | 146 | 99 | 2 | 46 | 189 | 56 | 201 | 1.20e-16 | 77.4 |
| MsG0380015749.01.T01 | MTR_5g069590 | 34.951 | 103 | 40 | 1 | 95 | 197 | 27 | 102 | 8.07e-13 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015749.01.T01 | AT4G12690 | 47.458 | 177 | 93 | 0 | 12 | 188 | 7 | 183 | 2.04e-57 | 183 |
| MsG0380015749.01.T01 | AT4G12690 | 47.458 | 177 | 93 | 0 | 12 | 188 | 7 | 183 | 2.04e-57 | 183 |
| MsG0380015749.01.T01 | AT5G48270 | 48.571 | 175 | 87 | 2 | 36 | 207 | 46 | 220 | 1.87e-55 | 179 |
| MsG0380015749.01.T01 | AT2G04220 | 45.833 | 168 | 91 | 0 | 26 | 193 | 25 | 192 | 1.36e-54 | 177 |
| MsG0380015749.01.T01 | AT3G04860 | 46.061 | 165 | 86 | 2 | 26 | 189 | 26 | 188 | 1.21e-46 | 156 |
| MsG0380015749.01.T01 | AT5G28150 | 41.935 | 186 | 103 | 2 | 7 | 189 | 4 | 187 | 5.67e-46 | 154 |
| MsG0380015749.01.T01 | AT3G13229 | 37.126 | 167 | 100 | 2 | 35 | 196 | 11 | 177 | 1.69e-31 | 117 |
| MsG0380015749.01.T01 | AT2G27770 | 36.628 | 172 | 103 | 4 | 23 | 189 | 41 | 211 | 8.34e-28 | 107 |
| MsG0380015749.01.T01 | AT5G11000 | 39.181 | 171 | 81 | 6 | 30 | 190 | 45 | 202 | 2.52e-22 | 94.0 |
| MsG0380015749.01.T01 | AT2G25200 | 38.971 | 136 | 70 | 3 | 73 | 206 | 100 | 224 | 4.26e-14 | 70.5 |
Find 34 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAATCTTATCTCCATTTC+TGG | 0.151343 | 3:+74563535 | None:intergenic |
| AGCTAAAGCCATGGTACTTT+TGG | 0.227384 | 3:-74563943 | MsG0380015749.01.T01:CDS |
| GAAAAGCAGTAGATGTCTTT+TGG | 0.238935 | 3:-74563886 | MsG0380015749.01.T01:CDS |
| CAAGAGGTTGTGTTACTTCT+TGG | 0.333460 | 3:-74563791 | MsG0380015749.01.T01:CDS |
| TCAAAACGGTTCACGGTAGA+TGG | 0.358029 | 3:-74563908 | MsG0380015749.01.T01:CDS |
| AGTAATAATGTTGTGGAAGA+TGG | 0.389758 | 3:-74563593 | MsG0380015749.01.T01:CDS |
| AAAGAAGCTTACAGAAGAAC+TGG | 0.403673 | 3:-74563758 | MsG0380015749.01.T01:CDS |
| ACAACAATGCAAGACTCAAT+TGG | 0.431750 | 3:-74564181 | None:intergenic |
| GGTTCAAGTTCAACCAGAAA+TGG | 0.432818 | 3:-74563548 | MsG0380015749.01.T01:CDS |
| CATGGAGCTGTGATACGTTC+AGG | 0.452249 | 3:-74564115 | MsG0380015749.01.T01:CDS |
| GAAATGGAGATAAGATTTGA+TGG | 0.453482 | 3:-74563532 | None:intergenic |
| AAAGCTAAGTTTCATGAGAA+AGG | 0.470014 | 3:-74563659 | MsG0380015749.01.T01:CDS |
| CTTAAATCTGCAAAATTCAA+TGG | 0.472464 | 3:-74563860 | MsG0380015749.01.T01:CDS |
| AACAGTGGAAAACCCTGAAA+AGG | 0.498086 | 3:-74563990 | MsG0380015749.01.T01:CDS |
| CTAAGTTTCATGAGAAAGGT+AGG | 0.531945 | 3:-74563655 | MsG0380015749.01.T01:CDS |
| GGAGGAAACAAGGTTCAAAA+CGG | 0.533531 | 3:-74563922 | MsG0380015749.01.T01:CDS |
| GCTACATAGTACTCTGAAGT+TGG | 0.534790 | 3:+74563827 | None:intergenic |
| GATGGAGATTCTGTGATGAT+TGG | 0.539474 | 3:-74563575 | MsG0380015749.01.T01:CDS |
| ACAAGGTTCAAAACGGTTCA+CGG | 0.540912 | 3:-74563915 | MsG0380015749.01.T01:CDS |
| AGTGATGATCATCATCATCA+TGG | 0.553528 | 3:-74564133 | MsG0380015749.01.T01:CDS |
| AGGAAGTAGTAATAATGTTG+TGG | 0.564284 | 3:-74563600 | MsG0380015749.01.T01:CDS |
| GTATACAGAACAAAGTTAGC+TGG | 0.572020 | 3:-74564073 | MsG0380015749.01.T01:CDS |
| GATTCTGTGATGATTGGTGG+TGG | 0.579232 | 3:-74563569 | MsG0380015749.01.T01:CDS |
| ACCATGTGATTGTGATCAAA+CGG | 0.581994 | 3:+74564044 | None:intergenic |
| GGAGATTCTGTGATGATTGG+TGG | 0.589604 | 3:-74563572 | MsG0380015749.01.T01:CDS |
| TCTATAAAGTGTAAGAACAA+AGG | 0.602434 | 3:-74563620 | MsG0380015749.01.T01:CDS |
| AGAATTGGATCAATGAGTGA+TGG | 0.607496 | 3:+74563728 | None:intergenic |
| TCATGGTTTATCAGTAACAG+TGG | 0.618870 | 3:-74564005 | MsG0380015749.01.T01:CDS |
| TGTTTCCTCCAAAAGTACCA+TGG | 0.623832 | 3:+74563935 | None:intergenic |
| TTCAGAGTACTATGTAGCAG+TGG | 0.623936 | 3:-74563822 | MsG0380015749.01.T01:CDS |
| GGAAACTCAATATAGCTGCA+AGG | 0.641526 | 3:-74563969 | MsG0380015749.01.T01:CDS |
| AGCAGTGGTTTGTGATCAAG+AGG | 0.660033 | 3:-74563807 | MsG0380015749.01.T01:CDS |
| GCCGTTTGATCACAATCACA+TGG | 0.670373 | 3:-74564045 | MsG0380015749.01.T01:CDS |
| GCAAGGTTGAGCTAAAGCCA+TGG | 0.676173 | 3:-74563952 | MsG0380015749.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTAAATCTGCAAAATTCAA+TGG | - | Chr3:74563850-74563869 | MsG0380015749.01.T01:CDS | 25.0% |
| ! | TCAAAAAAGGAACACATATT+TGG | - | Chr3:74564009-74564028 | MsG0380015749.01.T01:CDS | 25.0% |
| ! | TCTATAAAGTGTAAGAACAA+AGG | - | Chr3:74564090-74564109 | MsG0380015749.01.T01:CDS | 25.0% |
| !! | CATTGAATTTTGCAGATTTA+AGG | + | Chr3:74563852-74563871 | None:intergenic | 25.0% |
| !! | TATATTGAGTTTCCTTTTCA+GGG | + | Chr3:74563735-74563754 | None:intergenic | 25.0% |
| !! | TCCAATTCTTGTTTCAAAAA+AGG | - | Chr3:74563996-74564015 | MsG0380015749.01.T01:CDS | 25.0% |
| !!! | TCCTTTTTTGAAACAAGAAT+TGG | + | Chr3:74564000-74564019 | None:intergenic | 25.0% |
| AAAGCTAAGTTTCATGAGAA+AGG | - | Chr3:74564051-74564070 | MsG0380015749.01.T01:CDS | 30.0% | |
| AGGAAGTAGTAATAATGTTG+TGG | - | Chr3:74564110-74564129 | MsG0380015749.01.T01:CDS | 30.0% | |
| AGTAATAATGTTGTGGAAGA+TGG | - | Chr3:74564117-74564136 | MsG0380015749.01.T01:CDS | 30.0% | |
| TCAAATCTTATCTCCATTTC+TGG | + | Chr3:74564178-74564197 | None:intergenic | 30.0% | |
| ! | AAAAGCAGTAGATGTCTTTT+GGG | - | Chr3:74563825-74563844 | MsG0380015749.01.T01:CDS | 30.0% |
| ! | CTATATTGAGTTTCCTTTTC+AGG | + | Chr3:74563736-74563755 | None:intergenic | 30.0% |
| AAAGAAGCTTACAGAAGAAC+TGG | - | Chr3:74563952-74563971 | MsG0380015749.01.T01:CDS | 35.0% | |
| ACCATGTGATTGTGATCAAA+CGG | + | Chr3:74563669-74563688 | None:intergenic | 35.0% | |
| AGAATTGGATCAATGAGTGA+TGG | + | Chr3:74563985-74564004 | None:intergenic | 35.0% | |
| AGTGATGATCATCATCATCA+TGG | - | Chr3:74563577-74563596 | MsG0380015749.01.T01:CDS | 35.0% | |
| CTAAGTTTCATGAGAAAGGT+AGG | - | Chr3:74564055-74564074 | MsG0380015749.01.T01:CDS | 35.0% | |
| GTATACAGAACAAAGTTAGC+TGG | - | Chr3:74563637-74563656 | MsG0380015749.01.T01:CDS | 35.0% | |
| TCATGGTTTATCAGTAACAG+TGG | - | Chr3:74563705-74563724 | MsG0380015749.01.T01:CDS | 35.0% | |
| TGCAAAAACTTGATGCTTCA+TGG | - | Chr3:74563688-74563707 | MsG0380015749.01.T01:CDS | 35.0% | |
| ! | GAAAAGCAGTAGATGTCTTT+TGG | - | Chr3:74563824-74563843 | MsG0380015749.01.T01:CDS | 35.0% |
| AACAGTGGAAAACCCTGAAA+AGG | - | Chr3:74563720-74563739 | MsG0380015749.01.T01:CDS | 40.0% | |
| ACAAGGTTCAAAACGGTTCA+CGG | - | Chr3:74563795-74563814 | MsG0380015749.01.T01:CDS | 40.0% | |
| CTTGCAGAAGAAAAACATGC+AGG | + | Chr3:74563559-74563578 | None:intergenic | 40.0% | |
| GATGGAGATTCTGTGATGAT+TGG | - | Chr3:74564135-74564154 | MsG0380015749.01.T01:CDS | 40.0% | |
| GGAAACTCAATATAGCTGCA+AGG | - | Chr3:74563741-74563760 | MsG0380015749.01.T01:CDS | 40.0% | |
| GGAGGAAACAAGGTTCAAAA+CGG | - | Chr3:74563788-74563807 | MsG0380015749.01.T01:CDS | 40.0% | |
| ! | AGCTAAAGCCATGGTACTTT+TGG | - | Chr3:74563767-74563786 | MsG0380015749.01.T01:CDS | 40.0% |
| ! | GGTTCAAGTTCAACCAGAAA+TGG | - | Chr3:74564162-74564181 | MsG0380015749.01.T01:CDS | 40.0% |
| ! | TAAAGCCATGGTACTTTTGG+AGG | - | Chr3:74563770-74563789 | MsG0380015749.01.T01:CDS | 40.0% |
| ! | TTCAGAGTACTATGTAGCAG+TGG | - | Chr3:74563888-74563907 | MsG0380015749.01.T01:CDS | 40.0% |
| !! | CAAGAGGTTGTGTTACTTCT+TGG | - | Chr3:74563919-74563938 | MsG0380015749.01.T01:CDS | 40.0% |
| !! | GCTACATAGTACTCTGAAGT+TGG | + | Chr3:74563886-74563905 | None:intergenic | 40.0% |
| !! | TGGTACTTTTGGAGGAAACA+AGG | - | Chr3:74563778-74563797 | MsG0380015749.01.T01:CDS | 40.0% |
| !! | TGTTTCCTCCAAAAGTACCA+TGG | + | Chr3:74563778-74563797 | None:intergenic | 40.0% |
| GATTCTGTGATGATTGGTGG+TGG | - | Chr3:74564141-74564160 | MsG0380015749.01.T01:CDS | 45.0% | |
| GCCGTTTGATCACAATCACA+TGG | - | Chr3:74563665-74563684 | MsG0380015749.01.T01:CDS | 45.0% | |
| GGAGATTCTGTGATGATTGG+TGG | - | Chr3:74564138-74564157 | MsG0380015749.01.T01:CDS | 45.0% | |
| TCAAAACGGTTCACGGTAGA+TGG | - | Chr3:74563802-74563821 | MsG0380015749.01.T01:CDS | 45.0% | |
| ! | AGCAGTGGTTTGTGATCAAG+AGG | - | Chr3:74563903-74563922 | MsG0380015749.01.T01:CDS | 45.0% |
| CATGGAGCTGTGATACGTTC+AGG | - | Chr3:74563595-74563614 | MsG0380015749.01.T01:CDS | 50.0% | |
| GCAAGGTTGAGCTAAAGCCA+TGG | - | Chr3:74563758-74563777 | MsG0380015749.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 74563535 | 74564197 | 74563535 | ID=MsG0380015749.01;Name=MsG0380015749.01 |
| Chr3 | mRNA | 74563535 | 74564197 | 74563535 | ID=MsG0380015749.01.T01;Parent=MsG0380015749.01;Name=MsG0380015749.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|220 |
| Chr3 | exon | 74563535 | 74564197 | 74563535 | ID=MsG0380015749.01.T01:exon:24455;Parent=MsG0380015749.01.T01 |
| Chr3 | CDS | 74563535 | 74564197 | 74563535 | ID=MsG0380015749.01.T01:cds;Parent=MsG0380015749.01.T01 |
| Gene Sequence |
| Protein sequence |
