Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016179.01.T01 | XP_003601753.1 | 98.551 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 7.41E-39 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0380016179.01 | MsG0780037141.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016179.01.T01 | MTR_3g085020 | 98.551 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 8.97e-43 | 132 |
| MsG0380016179.01.T01 | MTR_8g096840 | 84.058 | 69 | 11 | 0 | 1 | 69 | 1 | 69 | 2.08e-37 | 119 |
| MsG0380016179.01.T01 | MTR_7g028975 | 65.217 | 69 | 24 | 0 | 1 | 69 | 1 | 69 | 2.67e-31 | 103 |
| MsG0380016179.01.T01 | MTR_8g097020 | 62.319 | 69 | 26 | 0 | 1 | 69 | 1 | 69 | 2.57e-30 | 101 |
| MsG0380016179.01.T01 | MTR_7g028870 | 65.152 | 66 | 23 | 0 | 2 | 67 | 3 | 68 | 9.55e-29 | 97.8 |
| MsG0380016179.01.T01 | MTR_8g027820 | 62.319 | 69 | 26 | 0 | 1 | 69 | 1 | 69 | 1.92e-28 | 98.2 |
| MsG0380016179.01.T01 | MTR_3g085030 | 59.420 | 69 | 28 | 0 | 1 | 69 | 1 | 69 | 5.82e-27 | 93.2 |
| MsG0380016179.01.T01 | MTR_8g097030 | 77.941 | 68 | 15 | 0 | 2 | 69 | 3 | 70 | 5.86e-26 | 90.5 |
| MsG0380016179.01.T01 | MTR_7g028940 | 57.971 | 69 | 29 | 0 | 1 | 69 | 1 | 69 | 1.89e-24 | 86.7 |
| MsG0380016179.01.T01 | MTR_7g028815 | 67.273 | 55 | 18 | 0 | 1 | 55 | 1 | 55 | 7.34e-23 | 84.3 |
| MsG0380016179.01.T01 | MTR_7g028945 | 56.863 | 51 | 22 | 0 | 1 | 51 | 1 | 51 | 1.64e-16 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016179.01.T01 | AT3G48660 | 81.159 | 69 | 13 | 0 | 1 | 69 | 1 | 69 | 1.77e-35 | 115 |
| MsG0380016179.01.T01 | AT5G63500 | 78.261 | 69 | 15 | 0 | 1 | 69 | 1 | 69 | 3.18e-33 | 108 |
| MsG0380016179.01.T01 | AT5G08391 | 62.319 | 69 | 26 | 0 | 1 | 69 | 1 | 69 | 2.72e-30 | 101 |
| MsG0380016179.01.T01 | AT3G27027 | 66.154 | 65 | 22 | 0 | 3 | 67 | 4 | 68 | 2.50e-28 | 96.7 |
| MsG0380016179.01.T01 | AT3G01950 | 61.194 | 67 | 26 | 0 | 3 | 69 | 4 | 70 | 5.38e-26 | 90.9 |
| MsG0380016179.01.T01 | AT5G40980 | 70.909 | 55 | 16 | 0 | 2 | 56 | 3 | 57 | 1.08e-25 | 90.1 |
| MsG0380016179.01.T01 | AT3G27030 | 62.121 | 66 | 25 | 0 | 1 | 66 | 1 | 66 | 2.55e-25 | 91.3 |
| MsG0380016179.01.T01 | AT3G27030 | 57.971 | 69 | 29 | 0 | 1 | 69 | 74 | 142 | 1.57e-24 | 89.4 |
| MsG0380016179.01.T01 | AT5G14110 | 57.812 | 64 | 27 | 0 | 3 | 66 | 4 | 67 | 4.78e-24 | 85.9 |
| MsG0380016179.01.T01 | AT3G01940 | 72.727 | 66 | 18 | 0 | 2 | 67 | 4 | 69 | 1.50e-23 | 84.7 |
| MsG0380016179.01.T01 | AT5G50560 | 62.903 | 62 | 23 | 0 | 1 | 62 | 1 | 62 | 5.12e-18 | 70.5 |
| MsG0380016179.01.T01 | AT5G50660 | 62.903 | 62 | 23 | 0 | 1 | 62 | 1 | 62 | 5.12e-18 | 70.5 |
| MsG0380016179.01.T01 | AT5G40960 | 45.455 | 66 | 36 | 0 | 1 | 66 | 1 | 66 | 1.08e-12 | 57.0 |
Find 19 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCAGTGGTGTGTCTATTT+TGG | 0.273169 | 3:-80023320 | MsG0380016179.01.T01:CDS |
| AGAAACAAAGTGGTGGAGTT+TGG | 0.286192 | 3:-80023352 | MsG0380016179.01.T01:CDS |
| TCCACCACTTTGTTTCTTCC+AGG | 0.330714 | 3:+80023358 | None:intergenic |
| ACCATCTTTCTTATTGCCAT+TGG | 0.390535 | 3:-80023268 | MsG0380016179.01.T01:CDS |
| GAAACAAAGTGGTGGAGTTT+GGG | 0.392874 | 3:-80023351 | MsG0380016179.01.T01:CDS |
| CTGTTTGTGTTGCTAAGTCC+AGG | 0.405516 | 3:-80023397 | MsG0380016179.01.T01:CDS |
| GGGTTGCTGTTTCAACTGCC+TGG | 0.426401 | 3:-80023376 | MsG0380016179.01.T01:CDS |
| ATTGGTGTGCATATCAATAC+TGG | 0.488306 | 3:-80023250 | MsG0380016179.01.T01:CDS |
| ACCAATGGCAATAAGAAAGA+TGG | 0.521238 | 3:+80023267 | None:intergenic |
| GGCAGTTGAAACAGCAACCC+TGG | 0.523057 | 3:+80023379 | None:intergenic |
| AGCACCACTGCTATTACCAC+TGG | 0.541172 | 3:+80023418 | None:intergenic |
| TGTTTGTGTTGCTAAGTCCA+GGG | 0.571919 | 3:-80023396 | MsG0380016179.01.T01:CDS |
| GACCAAAATAGACACACCAC+TGG | 0.627960 | 3:+80023318 | None:intergenic |
| GGGTCCAGTGGTAATAGCAG+TGG | 0.638939 | 3:-80023422 | MsG0380016179.01.T01:CDS |
| AGTATTGATATGCACACCAA+TGG | 0.648336 | 3:+80023252 | None:intergenic |
| GCCTGGAAGAAACAAAGTGG+TGG | 0.658875 | 3:-80023359 | MsG0380016179.01.T01:CDS |
| AATGGCGGATTGGGGTCCAG+TGG | 0.665904 | 3:-80023434 | MsG0380016179.01.T01:CDS |
| TTTGGGAACATGCAAACCAG+TGG | 0.682075 | 3:-80023334 | MsG0380016179.01.T01:CDS |
| ACTGCCTGGAAGAAACAAAG+TGG | 0.704719 | 3:-80023362 | MsG0380016179.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGAGTCCAAAAAAGAGGA+TGG | + | Chr3:80023391-80023410 | None:intergenic | 35.0% | |
| ACCAATGGCAATAAGAAAGA+TGG | + | Chr3:80023415-80023434 | None:intergenic | 35.0% | |
| ACCATCTTTCTTATTGCCAT+TGG | - | Chr3:80023411-80023430 | MsG0380016179.01.T01:CDS | 35.0% | |
| ATTGGTGTGCATATCAATAC+TGG | - | Chr3:80023429-80023448 | MsG0380016179.01.T01:CDS | 35.0% | |
| ! | TGGTAATGAGTCCAAAAAAG+AGG | + | Chr3:80023395-80023414 | None:intergenic | 35.0% |
| !! | AGTATTGATATGCACACCAA+TGG | + | Chr3:80023430-80023449 | None:intergenic | 35.0% |
| GTCCAAAAAAGAGGATGGTA+TGG | + | Chr3:80023386-80023405 | None:intergenic | 40.0% | |
| ! | AACCAGTGGTGTGTCTATTT+TGG | - | Chr3:80023359-80023378 | MsG0380016179.01.T01:CDS | 40.0% |
| ! | AGAAACAAAGTGGTGGAGTT+TGG | - | Chr3:80023327-80023346 | MsG0380016179.01.T01:CDS | 40.0% |
| ! | GAAACAAAGTGGTGGAGTTT+GGG | - | Chr3:80023328-80023347 | MsG0380016179.01.T01:CDS | 40.0% |
| ! | TGTTTGTGTTGCTAAGTCCA+GGG | - | Chr3:80023283-80023302 | MsG0380016179.01.T01:CDS | 40.0% |
| !!! | GTCCATACCATCCTCTTTTT+TGG | - | Chr3:80023381-80023400 | MsG0380016179.01.T01:CDS | 40.0% |
| ACTGCCTGGAAGAAACAAAG+TGG | - | Chr3:80023317-80023336 | MsG0380016179.01.T01:CDS | 45.0% | |
| GACCAAAATAGACACACCAC+TGG | + | Chr3:80023364-80023383 | None:intergenic | 45.0% | |
| TTTGGGAACATGCAAACCAG+TGG | - | Chr3:80023345-80023364 | MsG0380016179.01.T01:CDS | 45.0% | |
| ! | CTGTTTGTGTTGCTAAGTCC+AGG | - | Chr3:80023282-80023301 | MsG0380016179.01.T01:CDS | 45.0% |
| ! | TCCACCACTTTGTTTCTTCC+AGG | + | Chr3:80023324-80023343 | None:intergenic | 45.0% |
| AGCACCACTGCTATTACCAC+TGG | + | Chr3:80023264-80023283 | None:intergenic | 50.0% | |
| GCCTGGAAGAAACAAAGTGG+TGG | - | Chr3:80023320-80023339 | MsG0380016179.01.T01:CDS | 50.0% | |
| GGCAGTTGAAACAGCAACCC+TGG | + | Chr3:80023303-80023322 | None:intergenic | 55.0% | |
| GGGTCCAGTGGTAATAGCAG+TGG | - | Chr3:80023257-80023276 | MsG0380016179.01.T01:CDS | 55.0% | |
| !! | GGGTTGCTGTTTCAACTGCC+TGG | - | Chr3:80023303-80023322 | MsG0380016179.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 80023246 | 80023455 | 80023246 | ID=MsG0380016179.01;Name=MsG0380016179.01 |
| Chr3 | mRNA | 80023246 | 80023455 | 80023246 | ID=MsG0380016179.01.T01;Parent=MsG0380016179.01;Name=MsG0380016179.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|0|1|0|69 |
| Chr3 | exon | 80023246 | 80023455 | 80023246 | ID=MsG0380016179.01.T01:exon:32765;Parent=MsG0380016179.01.T01 |
| Chr3 | CDS | 80023246 | 80023455 | 80023246 | ID=MsG0380016179.01.T01:cds;Parent=MsG0380016179.01.T01 |
| Gene Sequence |
| Protein sequence |
