AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016320.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016320.01.T01 MTR_3g087760 96.936 359 9 1 1 357 1 359 0.0 717
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016320.01.T01 AT4G39820 67.630 346 107 1 16 356 81 426 0.0 511
MsG0380016320.01.T01 AT4G39820 67.630 346 107 1 16 356 62 407 0.0 509

Find 72 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTGATTCCGATTAAATT+AGG 0.137976 3:-81956723 MsG0380016320.01.T01:CDS
AACACGATTAGGGTAGATTT+TGG 0.205626 3:+81956782 None:intergenic
AAACAAAGCTTTGATTTATA+TGG 0.239354 3:-81956343 MsG0380016320.01.T01:CDS
TTCAGGATCACGCGAAATTA+AGG 0.260671 3:+81956506 None:intergenic
TTTGTTGTGAATTGTATAAT+TGG 0.271891 3:-81956579 MsG0380016320.01.T01:CDS
CTTTGATTCCGATTAAATTA+GGG 0.290041 3:-81956722 MsG0380016320.01.T01:CDS
TTCATTGAAACGACGTAGTT+TGG 0.322583 3:+81956869 None:intergenic
GAGGGAGAAGATTAAGGTTA+AGG 0.342982 3:-81956652 MsG0380016320.01.T01:CDS
GAGAGGGTCCGATTGGGTTT+CGG 0.354095 3:+81957061 None:intergenic
TGACGTTGCCCTAATTTAAT+CGG 0.359232 3:+81956714 None:intergenic
ACCATGGATCCAACACGATT+AGG 0.397351 3:+81956771 None:intergenic
CTCTTTGTTTGATGTATTTG+AGG 0.401632 3:-81956233 MsG0380016320.01.T01:CDS
TCCGATTGGGTTTCGGATCT+CGG 0.401999 3:+81957068 None:intergenic
ATCGTGGCAGAGTTCGTCTA+AGG 0.405157 3:+81957031 None:intergenic
AAGCTTTGATTTATATGGTT+GGG 0.409474 3:-81956338 MsG0380016320.01.T01:CDS
CGCCGCGGTTGGCTAGGTCT+TGG 0.419581 3:+81956997 None:intergenic
CCATGGATCCAACACGATTA+GGG 0.424835 3:+81956772 None:intergenic
GGGTATATTCAATTGCAAAC+TGG 0.430766 3:-81956459 MsG0380016320.01.T01:CDS
GCCACGCGCCGCGGTTGGCT+AGG 0.432138 3:+81956991 None:intergenic
TTAAGAGAACGCTTAGTAGT+TGG 0.433788 3:-81956083 MsG0380016320.01.T01:CDS
TTGCAAACTGGTGATTTAGA+AGG 0.434324 3:-81956447 MsG0380016320.01.T01:CDS
TAAGTGCTTCTGTTCGTGTA+TGG 0.435706 3:-81956617 MsG0380016320.01.T01:CDS
CCCTAATCGTGTTGGATCCA+TGG 0.436621 3:-81956772 MsG0380016320.01.T01:CDS
TGGGCACCATTTGAGTCATA+AGG 0.437788 3:-81956559 MsG0380016320.01.T01:CDS
CATTTGAGTCATAAGGAATT+CGG 0.449517 3:-81956552 MsG0380016320.01.T01:CDS
AAAGCTTTGATTTATATGGT+TGG 0.464398 3:-81956339 MsG0380016320.01.T01:CDS
TGGTTCCTTTCTCGCTTCGA+TGG 0.465126 3:-81956752 MsG0380016320.01.T01:CDS
GTTGATGAGAGGGTCCGATT+GGG 0.468045 3:+81957055 None:intergenic
TTCCAACACCTTGATCGAAT+CGG 0.468199 3:+81956203 None:intergenic
TCTTTGTTTGATGTATTTGA+GGG 0.471580 3:-81956232 MsG0380016320.01.T01:CDS
CCCGATACTCACAATGGAAT+CGG 0.476944 3:-81957096 None:intergenic
CGAATTCCTTATGACTCAAA+TGG 0.481735 3:+81956553 None:intergenic
AAAGCTGAAGGGAAGAATCA+TGG 0.481856 3:-81956405 MsG0380016320.01.T01:CDS
GGTTGATGAGAGGGTCCGAT+TGG 0.485202 3:+81957054 None:intergenic
TTGTTGTGAATTGTATAATT+GGG 0.485883 3:-81956578 MsG0380016320.01.T01:CDS
TCCGAGATCCGAAACCCAAT+CGG 0.491000 3:-81957069 MsG0380016320.01.T01:CDS
TGAACGCCACGCGCCGCGGT+TGG 0.501069 3:+81956986 None:intergenic
TGAAGCCATCGAAGCGAGAA+AGG 0.502353 3:+81956747 None:intergenic
TTGCAGAAGAGAGAGGGCTC+TGG 0.502503 3:+81956941 None:intergenic
CACGTTGAATCAAAATCATC+GGG 0.502703 3:+81956045 None:intergenic
GGATTTGTCCGATTCGATCA+AGG 0.503933 3:-81956211 MsG0380016320.01.T01:CDS
TGTTGGAAAATGCTCTTGAA+AGG 0.505906 3:-81956188 MsG0380016320.01.T01:CDS
GTCCGATTCGATCAAGGTGT+TGG 0.506959 3:-81956205 MsG0380016320.01.T01:CDS
GTTGGAAAATGCTCTTGAAA+GGG 0.519791 3:-81956187 MsG0380016320.01.T01:CDS
TTTGATTTATATGGTTGGGA+AGG 0.528211 3:-81956334 MsG0380016320.01.T01:CDS
ACACGTTGAATCAAAATCAT+CGG 0.538336 3:+81956044 None:intergenic
ACCTAGCCAACCGCGGCGCG+TGG 0.547585 3:-81956992 MsG0380016320.01.T01:CDS
AAAATCTACCCTAATCGTGT+TGG 0.547995 3:-81956780 MsG0380016320.01.T01:CDS
CGTCTAAGGGGTTGATGAGA+GGG 0.551260 3:+81957045 None:intergenic
TTTGTGTAGTATGTATGAGT+TGG 0.567364 3:-81956127 MsG0380016320.01.T01:CDS
TCGTGGCAGAGTTCGTCTAA+GGG 0.582317 3:+81957032 None:intergenic
TTAATTTCGCGTGATCCTGA+AGG 0.582488 3:-81956504 MsG0380016320.01.T01:CDS
TGATTGAACGCCACGCGCCG+CGG 0.583650 3:+81956982 None:intergenic
TGGCTAGGTCTTGGAGAGAG+TGG 0.586969 3:+81957006 None:intergenic
AGTATGATGAGTGCATTGAG+AGG 0.588619 3:-81956290 MsG0380016320.01.T01:CDS
GGATTATGTATCTGCTGTGA+GGG 0.590873 3:-81956313 MsG0380016320.01.T01:CDS
AAGCTGAAGGGAAGAATCAT+GGG 0.598988 3:-81956404 MsG0380016320.01.T01:CDS
CTTGGAGAGAGTGGAGATCG+TGG 0.599995 3:+81957015 None:intergenic
TCGTCTAAGGGGTTGATGAG+AGG 0.600298 3:+81957044 None:intergenic
AAGCTTTGTTTCTATTCACA+AGG 0.608648 3:+81956355 None:intergenic
TGAACTATCGAGATCTTCAA+CGG 0.617409 3:+81956827 None:intergenic
CGTTGAAAGCGAGGTATGTG+AGG 0.620749 3:+81956904 None:intergenic
ATGCAAGAGCGTTGAAAGCG+AGG 0.625123 3:+81956895 None:intergenic
CGTGGCAGAGTTCGTCTAAG+GGG 0.630480 3:+81957033 None:intergenic
GTATGATGAGTGCATTGAGA+GGG 0.635283 3:-81956289 MsG0380016320.01.T01:CDS
TCGAAGCGAGAAAGGAACCA+TGG 0.642727 3:+81956755 None:intergenic
GAATCATGGGTCGTTGAGCG+AGG 0.646671 3:-81956391 MsG0380016320.01.T01:CDS
AAATTAGGGCAACGTCAACA+AGG 0.652591 3:-81956708 MsG0380016320.01.T01:CDS
AGGATTATGTATCTGCTGTG+AGG 0.668001 3:-81956314 MsG0380016320.01.T01:CDS
CTCCAAGACCTAGCCAACCG+CGG 0.673129 3:-81956999 MsG0380016320.01.T01:CDS
GTCTCATTGAGAGCAACAGT+AGG 0.677132 3:+81956162 None:intergenic
AGGTATGTGAGGTGATCGTG+AGG 0.691631 3:+81956915 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTTGTGAATTGTATAATT+GGG - Chr3:81956537-81956556 MsG0380016320.01.T01:CDS 20.0%
!!! AAACAAAGCTTTGATTTATA+TGG - Chr3:81956772-81956791 MsG0380016320.01.T01:CDS 20.0%
!!! TTTGTTGTGAATTGTATAAT+TGG - Chr3:81956536-81956555 MsG0380016320.01.T01:CDS 20.0%
! CTTTGATTCCGATTAAATTA+GGG - Chr3:81956393-81956412 MsG0380016320.01.T01:CDS 25.0%
!! AAAGCTTTGATTTATATGGT+TGG - Chr3:81956776-81956795 MsG0380016320.01.T01:CDS 25.0%
!! AAGCTTTGATTTATATGGTT+GGG - Chr3:81956777-81956796 MsG0380016320.01.T01:CDS 25.0%
!! ATAAACTTCTAGATTTTGTG+AGG - Chr3:81956444-81956463 MsG0380016320.01.T01:CDS 25.0%
!! TAAACTTCTAGATTTTGTGA+GGG - Chr3:81956445-81956464 MsG0380016320.01.T01:CDS 25.0%
!! TCTTTGTTTGATGTATTTGA+GGG - Chr3:81956883-81956902 MsG0380016320.01.T01:CDS 25.0%
ACACGTTGAATCAAAATCAT+CGG + Chr3:81957074-81957093 None:intergenic 30.0%
CATTTGAGTCATAAGGAATT+CGG - Chr3:81956563-81956582 MsG0380016320.01.T01:CDS 30.0%
GCTTTGATTCCGATTAAATT+AGG - Chr3:81956392-81956411 MsG0380016320.01.T01:CDS 30.0%
TTTGTGTAGTATGTATGAGT+TGG - Chr3:81956988-81957007 MsG0380016320.01.T01:CDS 30.0%
! CTCTTTGTTTGATGTATTTG+AGG - Chr3:81956882-81956901 MsG0380016320.01.T01:CDS 30.0%
! TTTGATTTATATGGTTGGGA+AGG - Chr3:81956781-81956800 MsG0380016320.01.T01:CDS 30.0%
!! AAGCTTTGTTTCTATTCACA+AGG + Chr3:81956763-81956782 None:intergenic 30.0%
!! ATTCGTTTTTGAAAGCTGAA+GGG - Chr3:81956699-81956718 MsG0380016320.01.T01:CDS 30.0%
AAAATCTACCCTAATCGTGT+TGG - Chr3:81956335-81956354 MsG0380016320.01.T01:CDS 35.0%
CACGTTGAATCAAAATCATC+GGG + Chr3:81957073-81957092 None:intergenic 35.0%
CCAAGTTGCGAAATCAAAAA+CGG + Chr3:81956638-81956657 None:intergenic 35.0%
CGAATTCCTTATGACTCAAA+TGG + Chr3:81956565-81956584 None:intergenic 35.0%
GGGTATATTCAATTGCAAAC+TGG - Chr3:81956656-81956675 MsG0380016320.01.T01:CDS 35.0%
GTTGGAAAATGCTCTTGAAA+GGG - Chr3:81956928-81956947 MsG0380016320.01.T01:CDS 35.0%
TGAACTATCGAGATCTTCAA+CGG + Chr3:81956291-81956310 None:intergenic 35.0%
TGACGTTGCCCTAATTTAAT+CGG + Chr3:81956404-81956423 None:intergenic 35.0%
TGTTGGAAAATGCTCTTGAA+AGG - Chr3:81956927-81956946 MsG0380016320.01.T01:CDS 35.0%
TTAAGAGAACGCTTAGTAGT+TGG - Chr3:81957032-81957051 MsG0380016320.01.T01:CDS 35.0%
TTCATTGAAACGACGTAGTT+TGG + Chr3:81956249-81956268 None:intergenic 35.0%
TTGCAAACTGGTGATTTAGA+AGG - Chr3:81956668-81956687 MsG0380016320.01.T01:CDS 35.0%
! AACACGATTAGGGTAGATTT+TGG + Chr3:81956336-81956355 None:intergenic 35.0%
! ACACGATTAGGGTAGATTTT+GGG + Chr3:81956335-81956354 None:intergenic 35.0%
! TTTTGTGAGGGAGAAGATTA+AGG - Chr3:81956457-81956476 MsG0380016320.01.T01:CDS 35.0%
!! GATTCGTTTTTGAAAGCTGA+AGG - Chr3:81956698-81956717 MsG0380016320.01.T01:CDS 35.0%
!!! CGTTTTTGATTTCGCAACTT+GGG - Chr3:81956636-81956655 MsG0380016320.01.T01:CDS 35.0%
AAAGCTGAAGGGAAGAATCA+TGG - Chr3:81956710-81956729 MsG0380016320.01.T01:CDS 40.0%
AAATTAGGGCAACGTCAACA+AGG - Chr3:81956407-81956426 MsG0380016320.01.T01:CDS 40.0%
AAGCTGAAGGGAAGAATCAT+GGG - Chr3:81956711-81956730 MsG0380016320.01.T01:CDS 40.0%
AGGATTATGTATCTGCTGTG+AGG - Chr3:81956801-81956820 MsG0380016320.01.T01:CDS 40.0%
AGTATGATGAGTGCATTGAG+AGG - Chr3:81956825-81956844 MsG0380016320.01.T01:CDS 40.0%
GAAATCAAAAACGGACCTTC+AGG + Chr3:81956629-81956648 None:intergenic 40.0%
GAGGGAGAAGATTAAGGTTA+AGG - Chr3:81956463-81956482 MsG0380016320.01.T01:CDS 40.0%
GGATTATGTATCTGCTGTGA+GGG - Chr3:81956802-81956821 MsG0380016320.01.T01:CDS 40.0%
GTATGATGAGTGCATTGAGA+GGG - Chr3:81956826-81956845 MsG0380016320.01.T01:CDS 40.0%
TAAGTGCTTCTGTTCGTGTA+TGG - Chr3:81956498-81956517 MsG0380016320.01.T01:CDS 40.0%
TTAATTTCGCGTGATCCTGA+AGG - Chr3:81956611-81956630 MsG0380016320.01.T01:CDS 40.0%
TTCAGGATCACGCGAAATTA+AGG + Chr3:81956612-81956631 None:intergenic 40.0%
TTCCAACACCTTGATCGAAT+CGG + Chr3:81956915-81956934 None:intergenic 40.0%
! CACGATTAGGGTAGATTTTG+GGG + Chr3:81956334-81956353 None:intergenic 40.0%
!!! ATTAGGGTAGATTTTGGGGT+AGG + Chr3:81956330-81956349 None:intergenic 40.0%
!!! CCGTTTTTGATTTCGCAACT+TGG - Chr3:81956635-81956654 MsG0380016320.01.T01:CDS 40.0%
!!! TTTTGGGGTAGGTTTCGTAA+CGG + Chr3:81956319-81956338 None:intergenic 40.0%
ACCATGGATCCAACACGATT+AGG + Chr3:81956347-81956366 None:intergenic 45.0%
CCATGGATCCAACACGATTA+GGG + Chr3:81956346-81956365 None:intergenic 45.0%
GTCTCATTGAGAGCAACAGT+AGG + Chr3:81956956-81956975 None:intergenic 45.0%
TCGTGTATGGCGAAAAAGAG+AGG - Chr3:81956511-81956530 MsG0380016320.01.T01:CDS 45.0%
TGGGCACCATTTGAGTCATA+AGG - Chr3:81956556-81956575 MsG0380016320.01.T01:CDS 45.0%
! AGGCTTTTGCAGAAGAGAGA+GGG + Chr3:81956183-81956202 None:intergenic 45.0%
! GGATTTGTCCGATTCGATCA+AGG - Chr3:81956904-81956923 MsG0380016320.01.T01:CDS 45.0%
AGGTATGTGAGGTGATCGTG+AGG + Chr3:81956203-81956222 None:intergenic 50.0%
ATCGTGGCAGAGTTCGTCTA+AGG + Chr3:81956087-81956106 None:intergenic 50.0%
CCCTAATCGTGTTGGATCCA+TGG - Chr3:81956343-81956362 MsG0380016320.01.T01:CDS 50.0%
CGTTGAAAGCGAGGTATGTG+AGG + Chr3:81956214-81956233 None:intergenic 50.0%
TCCGAGATCCGAAACCCAAT+CGG - Chr3:81956046-81956065 MsG0380016320.01.T01:CDS 50.0%
TCCGATTGGGTTTCGGATCT+CGG + Chr3:81956050-81956069 None:intergenic 50.0%
TCGAAGCGAGAAAGGAACCA+TGG + Chr3:81956363-81956382 None:intergenic 50.0%
TCGTGGCAGAGTTCGTCTAA+GGG + Chr3:81956086-81956105 None:intergenic 50.0%
TGAAGCCATCGAAGCGAGAA+AGG + Chr3:81956371-81956390 None:intergenic 50.0%
TGGTTCCTTTCTCGCTTCGA+TGG - Chr3:81956363-81956382 MsG0380016320.01.T01:CDS 50.0%
! GAGGCTTTTGCAGAAGAGAG+AGG + Chr3:81956184-81956203 None:intergenic 50.0%
! GTCCGATTCGATCAAGGTGT+TGG - Chr3:81956910-81956929 MsG0380016320.01.T01:CDS 50.0%
! GTTGATGAGAGGGTCCGATT+GGG + Chr3:81956063-81956082 None:intergenic 50.0%
!! ATGCAAGAGCGTTGAAAGCG+AGG + Chr3:81956223-81956242 None:intergenic 50.0%
!! CGTCTAAGGGGTTGATGAGA+GGG + Chr3:81956073-81956092 None:intergenic 50.0%
!! TCGTCTAAGGGGTTGATGAG+AGG + Chr3:81956074-81956093 None:intergenic 50.0%
CGTGGCAGAGTTCGTCTAAG+GGG + Chr3:81956085-81956104 None:intergenic 55.0%
CTTGGAGAGAGTGGAGATCG+TGG + Chr3:81956103-81956122 None:intergenic 55.0%
GAATCATGGGTCGTTGAGCG+AGG - Chr3:81956724-81956743 MsG0380016320.01.T01:CDS 55.0%
GAGAGGGTCCGATTGGGTTT+CGG + Chr3:81956057-81956076 None:intergenic 55.0%
TGGCTAGGTCTTGGAGAGAG+TGG + Chr3:81956112-81956131 None:intergenic 55.0%
TTGCAGAAGAGAGAGGGCTC+TGG + Chr3:81956177-81956196 None:intergenic 55.0%
!! GGTTGATGAGAGGGTCCGAT+TGG + Chr3:81956064-81956083 None:intergenic 55.0%
CTCCAAGACCTAGCCAACCG+CGG - Chr3:81956116-81956135 MsG0380016320.01.T01:CDS 60.0%
TGATTGAACGCCACGCGCCG+CGG + Chr3:81956136-81956155 None:intergenic 65.0%
! CGCCGCGGTTGGCTAGGTCT+TGG + Chr3:81956121-81956140 None:intergenic 70.0%
ACCTAGCCAACCGCGGCGCG+TGG - Chr3:81956123-81956142 MsG0380016320.01.T01:CDS 75.0%
TGAACGCCACGCGCCGCGGT+TGG + Chr3:81956132-81956151 None:intergenic 75.0%
!! GCCACGCGCCGCGGTTGGCT+AGG + Chr3:81956127-81956146 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr3 gene 81956032 81957105 81956032 ID=MsG0380016320.01;Name=MsG0380016320.01
Chr3 mRNA 81956032 81957105 81956032 ID=MsG0380016320.01.T01;Parent=MsG0380016320.01;Name=MsG0380016320.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|357
Chr3 exon 81956032 81957105 81956032 ID=MsG0380016320.01.T01:exon:11668;Parent=MsG0380016320.01.T01
Chr3 CDS 81956032 81957105 81956032 ID=MsG0380016320.01.T01:cds;Parent=MsG0380016320.01.T01
Gene Sequence

>MsG0380016320.01.T01

ATGGAATCGGATTCTCCGAGATCCGAAACCCAATCGGACCCTCTCATCAACCCCTTAGACGAACTCTGCCACGATCTCCACTCTCTCCAAGACCTAGCCAACCGCGGCGCGTGGCGTTCAATCATCGACAAAGTCGCACGTGCCAGAGCCCTCTCTCTTCTGCAAAAGCCTCACGATCACCTCACATACCTCGCTTTCAACGCTCTTGCATTCACCAAACTACGTCGTTTCAATGAAGCTTCTTCAGAACTCGATTCCGTTGAAGATCTCGATAGTTCACATTACCGTTACGAAACCTACCCCAAAATCTACCCTAATCGTGTTGGATCCATGGTTCCTTTCTCGCTTCGATGGCTTCATGCTTTGATTCCGATTAAATTAGGGCAACGTCAACAAGGAATTGATCGATTGTATAAACTTCTAGATTTTGTGAGGGAGAAGATTAAGGTTAAGGAAGATGATAATTTAAGTGCTTCTGTTCGTGTATGGCGAAAAAGAGAGGTTTTTGTTGTGAATTGTATAATTGGGCACCATTTGAGTCATAAGGAATTCGGTGTGTGTTTGAGTTTGATCAAAACCTTAATTTCGCGTGATCCTGAAGGTCCGTTTTTGATTTCGCAACTTGGGTATATTCAATTGCAAACTGGTGATTTAGAAGGTGCGAAAGATTCGTTTTTGAAAGCTGAAGGGAAGAATCATGGGTCGTTGAGCGAGGTTGAGTTTAAGAACCTTGTGAATAGAAACAAAGCTTTGATTTATATGGTTGGGAAGGATTATGTATCTGCTGTGAGGGAGTATGATGAGTGCATTGAGAGGGATCATAGTGATATAGTTGCTTTTAATAATAAAGCTCTTTGTTTGATGTATTTGAGGGATTTGTCCGATTCGATCAAGGTGTTGGAAAATGCTCTTGAAAGGGTTCCTACTGTTGCTCTCAATGAGACTCTTGTTGTGAATTTGTGTAGTATGTATGAGTTGGCTTATGTTAATCATTCTGATATTAAGAGAACGCTTAGTAGTTGGATTGCTCGCGTTGCTCCCGATGATTTTGATTCAACGTGTACTCGTACATGA

Protein sequence

>MsG0380016320.01.T01

MESDSPRSETQSDPLINPLDELCHDLHSLQDLANRGAWRSIIDKVARARALSLLQKPHDHLTYLAFNALAFTKLRRFNEASSELDSVEDLDSSHYRYETYPKIYPNRVGSMVPFSLRWLHALIPIKLGQRQQGIDRLYKLLDFVREKIKVKEDDNLSASVRVWRKREVFVVNCIIGHHLSHKEFGVCLSLIKTLISRDPEGPFLISQLGYIQLQTGDLEGAKDSFLKAEGKNHGSLSEVEFKNLVNRNKALIYMVGKDYVSAVREYDECIERDHSDIVAFNNKALCLMYLRDLSDSIKVLENALERVPTVALNETLVVNLCSMYELAYVNHSDIKRTLSSWIARVAPDDFDSTCTRT*