AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016754.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016754.01.T01 MTR_3g094800 71.329 143 30 5 1 136 1 139 6.27e-46 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAGCATTTCTCAATGTTT+TGG 0.252847 3:-87449895 MsG0380016754.01.T01:CDS
ATAGAATTCCATGGTTGATT+TGG 0.273453 3:-87454228 MsG0380016754.01.T01:CDS
CAAATCAGAAATGTAGAAAA+AGG 0.305245 3:-87449847 MsG0380016754.01.T01:CDS
TGTTGAGGGTTAGTTGAAAC+AGG 0.316995 3:+87454049 None:intergenic
GAAATGCTACAGTGGCTAAT+TGG 0.323795 3:+87449907 None:intergenic
CGCCACCGTCTCTAACTTGC+CGG 0.365914 3:-87454137 MsG0380016754.01.T01:CDS
GAAGAGGCGGAGGAAGATTC+CGG 0.370959 3:+87454118 None:intergenic
CTATTTCACCTGCAATACTT+AGG 0.411380 3:+87450007 None:intergenic
CTTCCTCCGCCTCTTCTAAA+GGG 0.425150 3:-87454111 MsG0380016754.01.T01:CDS
TCTTCCTCCGCCTCTTCTAA+AGG 0.425822 3:-87454112 MsG0380016754.01.T01:CDS
GGACAGAACCTAAGTATTGC+AGG 0.430483 3:-87450015 MsG0380016754.01.T01:CDS
GAGAAGTGGAAGATTAATGA+GGG 0.450082 3:-87449968 MsG0380016754.01.T01:CDS
TGAAGATATAAAAGAGTGCA+AGG 0.483511 3:+87454179 None:intergenic
GTGTAATATTGCGGTGTTGA+GGG 0.500957 3:+87454035 None:intergenic
AGAGTGCAAGGAGGAGAAAG+AGG 0.525954 3:+87454191 None:intergenic
ACATGTTCAAACATGAGAAG+TGG 0.542290 3:-87449982 MsG0380016754.01.T01:CDS
TGAGAAGTGGAAGATTAATG+AGG 0.556356 3:-87449969 MsG0380016754.01.T01:CDS
CAGAAAGCTATAGAATTCCA+TGG 0.560898 3:-87454237 MsG0380016754.01.T01:CDS
AAACCCTTTAGAAGAGGCGG+AGG 0.566769 3:+87454108 None:intergenic
ACACCGCAATATTACACAGC+AGG 0.587519 3:-87454029 MsG0380016754.01.T01:intron
GAAAAGAAACCCTTTAGAAG+AGG 0.600276 3:+87454102 None:intergenic
AAGAAACCCTTTAGAAGAGG+CGG 0.610911 3:+87454105 None:intergenic
TGATTTGATGAAGAATCCGA+CGG 0.611434 3:+87449863 None:intergenic
AGAAGTGGAAGATTAATGAG+GGG 0.612940 3:-87449967 MsG0380016754.01.T01:CDS
GATTTGATGAAGAATCCGAC+GGG 0.627656 3:+87449864 None:intergenic
TGTGTAATATTGCGGTGTTG+AGG 0.629031 3:+87454034 None:intergenic
GTTGAGGGTTAGTTGAAACA+GGG 0.629774 3:+87454050 None:intergenic
AGATATAAAAGAGTGCAAGG+AGG 0.631266 3:+87454182 None:intergenic
CGGCAAGTTAGAGACGGTGG+CGG 0.643629 3:+87454138 None:intergenic
TTCCGGCAAGTTAGAGACGG+TGG 0.646827 3:+87454135 None:intergenic
AGTTGAATCCAAATCAACCA+TGG 0.651961 3:+87454220 None:intergenic
AACATTGAGAAATGCTACAG+TGG 0.652369 3:+87449899 None:intergenic
TAACCTGCTGTGTAATATTG+CGG 0.658986 3:+87454026 None:intergenic
AGATTCCGGCAAGTTAGAGA+CGG 0.660394 3:+87454132 None:intergenic
TTGAGGGTTAGTTGAAACAG+GGG 0.665802 3:+87454051 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATTATTAAAAAATAA+AGG + Chr3:87451181-87451200 None:intergenic 0.0%
!!! TAATAAATTAATTAATTTTT+TGG + Chr3:87450433-87450452 None:intergenic 0.0%
!! ATAATAATGTATATATTGAT+CGG + Chr3:87453164-87453183 None:intergenic 10.0%
!! ATATATTAATAAACTATATG+TGG - Chr3:87452710-87452729 MsG0380016754.01.T01:intron 10.0%
!! ATATATTCAAAAAATATATG+TGG - Chr3:87452226-87452245 MsG0380016754.01.T01:intron 10.0%
!! TATATTAATAAACTATATGT+GGG - Chr3:87452711-87452730 MsG0380016754.01.T01:intron 10.0%
!! TATATTCAAAAAATATATGT+GGG - Chr3:87452227-87452246 MsG0380016754.01.T01:intron 10.0%
!! TGAATATATTAATAAAACTT+CGG + Chr3:87452215-87452234 None:intergenic 10.0%
!!! ACATTTTAAAAAAAAAACAA+CGG + Chr3:87452073-87452092 None:intergenic 10.0%
!!! ATTATTTTACTTTTTTTTGA+AGG - Chr3:87451485-87451504 MsG0380016754.01.T01:intron 10.0%
!!! TAAATTAATTAATTTTTTGG+TGG + Chr3:87450430-87450449 None:intergenic 10.0%
!!! TATATTATTATGATTTTTCA+CGG - Chr3:87452988-87453007 MsG0380016754.01.T01:intron 10.0%
!! AAAAAGTTTGAAAGAAAATT+AGG + Chr3:87450982-87451001 None:intergenic 15.0%
!! ATAAAATATATATGAGTGTA+TGG - Chr3:87452866-87452885 MsG0380016754.01.T01:intron 15.0%
!! ATATTCAAAAAATATATGTG+GGG - Chr3:87452228-87452247 MsG0380016754.01.T01:intron 15.0%
!! TAAAATATGAGCATAAATTT+AGG + Chr3:87453337-87453356 None:intergenic 15.0%
!!! ACTTATTATTTTAGGAAAAT+TGG - Chr3:87453514-87453533 MsG0380016754.01.T01:intron 15.0%
!!! ATATTATTATGATTTTTCAC+GGG - Chr3:87452989-87453008 MsG0380016754.01.T01:intron 15.0%
!!! ATATTGTATGTTTGAAATTA+TGG + Chr3:87453816-87453835 None:intergenic 15.0%
!!! ATCTCACTATATTTTTTTAT+GGG - Chr3:87452908-87452927 MsG0380016754.01.T01:intron 15.0%
!!! TATCTCACTATATTTTTTTA+TGG - Chr3:87452907-87452926 MsG0380016754.01.T01:intron 15.0%
!!! TATTTGGTACTTATTATTTT+AGG - Chr3:87453506-87453525 MsG0380016754.01.T01:intron 15.0%
!!! TCACTATATGTTTTTTTTTA+CGG - Chr3:87451930-87451949 MsG0380016754.01.T01:intron 15.0%
!!! TCATATATTTTTCTTTTGAA+AGG + Chr3:87452776-87452795 None:intergenic 15.0%
!!! TCTTATTTCTGAAATTTAAT+GGG - Chr3:87451849-87451868 MsG0380016754.01.T01:intron 15.0%
!!! TTCTTATTTCTGAAATTTAA+TGG - Chr3:87451848-87451867 MsG0380016754.01.T01:intron 15.0%
!! AAAACTATACTTGAATTTGT+TGG + Chr3:87450093-87450112 None:intergenic 20.0%
!! AAATAACCTTAAAGTTGTAA+AGG + Chr3:87451005-87451024 None:intergenic 20.0%
!! AAATGAAAAATGAAGAAAGT+TGG + Chr3:87451245-87451264 None:intergenic 20.0%
!! AAGTTCAATAGAAGAATTAT+TGG + Chr3:87453406-87453425 None:intergenic 20.0%
!! AATTTATGAAAAGTAAAGAG+TGG + Chr3:87453701-87453720 None:intergenic 20.0%
!! AATTTCATAGAATACTACTT+CGG + Chr3:87450854-87450873 None:intergenic 20.0%
!! AGTTGATTAGAAGAAAAATT+AGG + Chr3:87450051-87450070 None:intergenic 20.0%
!! GATAAATATATATGAGTGTA+TGG - Chr3:87452351-87452370 MsG0380016754.01.T01:intron 20.0%
!! GATGCATTTAAAAAAAACAA+CGG + Chr3:87452534-87452553 None:intergenic 20.0%
!! TCTTAAAAAGAAGAAATTGA+AGG - Chr3:87453539-87453558 MsG0380016754.01.T01:intron 20.0%
!! TTATTGTTTGACAAATTTGA+TGG - Chr3:87450666-87450685 MsG0380016754.01.T01:intron 20.0%
!! TTTCTCAAAAACAATAGTAA+AGG + Chr3:87450769-87450788 None:intergenic 20.0%
!! TTTGAAAGAAAATTAGGTAT+GGG + Chr3:87450976-87450995 None:intergenic 20.0%
!!! ACGTTAATTATGTTTTTTCA+CGG - Chr3:87451410-87451429 MsG0380016754.01.T01:intron 20.0%
!!! ACTCACTATATGTTTTTATA+CGG - Chr3:87452395-87452414 MsG0380016754.01.T01:intron 20.0%
!!! ATAAGCAATTTTGACTTTTT+AGG + Chr3:87450572-87450591 None:intergenic 20.0%
!!! ATGAGTATATGTTTGTTTTA+CGG - Chr3:87451810-87451829 MsG0380016754.01.T01:intron 20.0%
!!! ATGAGTATATGTTTGTTTTA+TGG - Chr3:87451810-87451829 MsG0380016754.01.T01:intron 20.0%
!!! CAATATAACCATTTTTTTAG+AGG - Chr3:87453830-87453849 MsG0380016754.01.T01:intron 20.0%
!!! TCTTATTTCTGAAATCTAAT+GGG - Chr3:87452317-87452336 MsG0380016754.01.T01:intron 20.0%
!!! TGAGTATATGTTTGTTTTAT+GGG - Chr3:87451811-87451830 MsG0380016754.01.T01:intron 20.0%
!!! TTCTCTACTAATTTTCAATT+CGG - Chr3:87451289-87451308 MsG0380016754.01.T01:intron 20.0%
!!! TTCTTATTTCTGAAATCTAA+TGG - Chr3:87452316-87452335 MsG0380016754.01.T01:intron 20.0%
!!! TTTTAGATTAGTTTGATCTA+TGG + Chr3:87450290-87450309 None:intergenic 20.0%
! AACTTTATCCTTTCTATGTT+AGG - Chr3:87453421-87453440 MsG0380016754.01.T01:intron 25.0%
! AATAAAGAAATGTGAGAAAG+TGG + Chr3:87451065-87451084 None:intergenic 25.0%
! ACTCGTTATAGTTACTAAAT+TGG + Chr3:87452597-87452616 None:intergenic 25.0%
! ACTTCTTAGAATAGATGAAT+TGG + Chr3:87453633-87453652 None:intergenic 25.0%
! ATAAAGAAATGTGAGAAAGT+GGG + Chr3:87451064-87451083 None:intergenic 25.0%
! ATAATATATATGTGGGTGTA+TGG - Chr3:87451764-87451783 MsG0380016754.01.T01:intron 25.0%
! CAAATCAGAAATGTAGAAAA+AGG - Chr3:87454198-87454217 MsG0380016754.01.T01:CDS 25.0%
! CATTCAATAAAAGATGTATG+TGG - Chr3:87450510-87450529 MsG0380016754.01.T01:intron 25.0%
! CATTCAATAAATGATGTATG+TGG + Chr3:87450547-87450566 None:intergenic 25.0%
! CTCGTTATAGTTACTAAATT+GGG + Chr3:87452596-87452615 None:intergenic 25.0%
! GAGTTTAACCTTGTAATTTA+AGG + Chr3:87450802-87450821 None:intergenic 25.0%
! GTAAGAAAAAGATAAGAGAT+TGG + Chr3:87451223-87451242 None:intergenic 25.0%
! GTTTGAAAGAAAATTAGGTA+TGG + Chr3:87450977-87450996 None:intergenic 25.0%
! TAAAACATAATTAATGCACG+CGG - Chr3:87450351-87450370 MsG0380016754.01.T01:intron 25.0%
! TAGAATAGATGAATTGGAAT+AGG + Chr3:87453627-87453646 None:intergenic 25.0%
! TATAGTAAAAACTACTTCCT+CGG + Chr3:87450604-87450623 None:intergenic 25.0%
! TGAACATACCTTAAATTACA+AGG - Chr3:87450791-87450810 MsG0380016754.01.T01:intron 25.0%
! TGATTATGCATTATAATTCG+AGG - Chr3:87453970-87453989 MsG0380016754.01.T01:intron 25.0%
!! AATTTGCTTATAATAGTGAC+CGG + Chr3:87450475-87450494 None:intergenic 25.0%
!! ATCTTTTGCATTAGAAGATT+GGG - Chr3:87453917-87453936 MsG0380016754.01.T01:intron 25.0%
!! CATATGAACCTCTAAAAAAA+TGG + Chr3:87453841-87453860 None:intergenic 25.0%
!! CATATTTTATGTGAGATAGT+GGG - Chr3:87453348-87453367 MsG0380016754.01.T01:intron 25.0%
!! CTTTTTCCTTTACAACTTTA+AGG - Chr3:87450996-87451015 MsG0380016754.01.T01:intron 25.0%
!! GTGTCACTAAAAAAAATCAT+TGG - Chr3:87452091-87452110 MsG0380016754.01.T01:intron 25.0%
!! TACTTTACATATTTTCACCA+TGG - Chr3:87453192-87453211 MsG0380016754.01.T01:intron 25.0%
!! TATCTTTTGCATTAGAAGAT+TGG - Chr3:87453916-87453935 MsG0380016754.01.T01:intron 25.0%
!! TCATATTTTATGTGAGATAG+TGG - Chr3:87453347-87453366 MsG0380016754.01.T01:intron 25.0%
!!! ATCATTTTTTCTCTCTTTCT+TGG + Chr3:87453954-87453973 None:intergenic 25.0%
!!! ATTGTAGAGTTTTTTGTTTC+AGG - Chr3:87450311-87450330 MsG0380016754.01.T01:intron 25.0%
!!! CGTTAATTATGTTTTTTCAC+GGG - Chr3:87451411-87451430 MsG0380016754.01.T01:intron 25.0%
!!! CTCACTATATGTTTTTATAC+GGG - Chr3:87452396-87452415 MsG0380016754.01.T01:intron 25.0%
!!! GTGAGTATATGTTTGTTTTA+CGG - Chr3:87452280-87452299 MsG0380016754.01.T01:intron 25.0%
!!! TCATTTTTTCTCTCTTTCTT+GGG + Chr3:87453953-87453972 None:intergenic 25.0%
!!! TGAGTATATGTTTGTTTTAC+GGG - Chr3:87452281-87452300 MsG0380016754.01.T01:intron 25.0%
AAAAAGAAGAAATTGAAGGC+TGG - Chr3:87453543-87453562 MsG0380016754.01.T01:intron 30.0%
ACTCGTTGTAGTTACTAAAA+TGG + Chr3:87451668-87451687 None:intergenic 30.0%
ATAGAATTCCATGGTTGATT+TGG - Chr3:87449817-87449836 MsG0380016754.01.T01:CDS 30.0%
ATAGTAAAAACTACTTCCTC+GGG + Chr3:87450603-87450622 None:intergenic 30.0%
ATCCTTTCTATGTTAGGAAA+AGG - Chr3:87453427-87453446 MsG0380016754.01.T01:intron 30.0%
CAAAGAACAATACTACTAGT+AGG + Chr3:87450824-87450843 None:intergenic 30.0%
CTCGTTGTAGTTACTAAAAT+GGG + Chr3:87451667-87451686 None:intergenic 30.0%
GCTGCTAGTATTTGATTATT+TGG - Chr3:87453490-87453509 MsG0380016754.01.T01:intron 30.0%
GTAGGTGATTGATGTATATT+TGG - Chr3:87453992-87454011 MsG0380016754.01.T01:intron 30.0%
TAATAAAACTTAGGCAATCG+CGG + Chr3:87451736-87451755 None:intergenic 30.0%
TATGCATTATAATTCGAGGT+AGG - Chr3:87453974-87453993 MsG0380016754.01.T01:intron 30.0%
TGTATCACTAAAAAATCCGT+TGG - Chr3:87451619-87451638 MsG0380016754.01.T01:intron 30.0%
! ATCCTTTTCCTAACATAGAA+AGG + Chr3:87453432-87453451 None:intergenic 30.0%
! TGAAGATATAAAAGAGTGCA+AGG + Chr3:87449869-87449888 None:intergenic 30.0%
! TGTAGCATTTCTCAATGTTT+TGG - Chr3:87454150-87454169 MsG0380016754.01.T01:CDS 30.0%
!! AAAGTGGGTTTTAGTAAAGT+GGG + Chr3:87451049-87451068 None:intergenic 30.0%
!!! CATAGTGATTTTTTTGTCCA+TGG + Chr3:87453212-87453231 None:intergenic 30.0%
!!! GTATATTTGGTTTTTCTAGC+AGG - Chr3:87454005-87454024 MsG0380016754.01.T01:intron 30.0%
!!! TTGATGGCTTTTAGAATACT+TGG - Chr3:87450682-87450701 MsG0380016754.01.T01:intron 30.0%
AACATTGAGAAATGCTACAG+TGG + Chr3:87454149-87454168 None:intergenic 35.0%
AATGTGTCTGTTTGATGTGT+AGG - Chr3:87453746-87453765 MsG0380016754.01.T01:intron 35.0%
ACATGTTCAAACATGAGAAG+TGG - Chr3:87454063-87454082 MsG0380016754.01.T01:CDS 35.0%
AGAAGTGGAAGATTAATGAG+GGG - Chr3:87454078-87454097 MsG0380016754.01.T01:CDS 35.0%
AGTTGAATCCAAATCAACCA+TGG + Chr3:87449828-87449847 None:intergenic 35.0%
ATCAAATACTAGCAGCATGT+TGG + Chr3:87453486-87453505 None:intergenic 35.0%
ATTGGAAAAAATGGCAAGTG+TGG + Chr3:87454123-87454142 None:intergenic 35.0%
CAGAAAGCTATAGAATTCCA+TGG - Chr3:87449808-87449827 MsG0380016754.01.T01:CDS 35.0%
CAGTGGCTAATTGGAAAAAA+TGG + Chr3:87454132-87454151 None:intergenic 35.0%
CTATTTCACCTGCAATACTT+AGG + Chr3:87454041-87454060 None:intergenic 35.0%
GAGAAGTGGAAGATTAATGA+GGG - Chr3:87454077-87454096 MsG0380016754.01.T01:CDS 35.0%
GTTACAATTGTACACTCCAA+CGG + Chr3:87451638-87451657 None:intergenic 35.0%
TAACCTGCTGTGTAATATTG+CGG + Chr3:87450022-87450041 None:intergenic 35.0%
TAATAAAACTTAGGCAGTCG+CGG + Chr3:87452690-87452709 None:intergenic 35.0%
TAGTAACTACAACGAGTGAT+CGG - Chr3:87451671-87451690 MsG0380016754.01.T01:intron 35.0%
TCTATGTTAGGAAAAGGATG+TGG - Chr3:87453433-87453452 MsG0380016754.01.T01:intron 35.0%
TGAGAAGTGGAAGATTAATG+AGG - Chr3:87454076-87454095 MsG0380016754.01.T01:CDS 35.0%
TGATTTGATGAAGAATCCGA+CGG + Chr3:87454185-87454204 None:intergenic 35.0%
TGCATCAATAAAAAACCCGT+TGG - Chr3:87452548-87452567 MsG0380016754.01.T01:intron 35.0%
TGGAGTGTACAATTGTAACA+CGG - Chr3:87451639-87451658 MsG0380016754.01.T01:intron 35.0%
TGTTTCGCAGTAATCATAAG+AGG - Chr3:87453575-87453594 MsG0380016754.01.T01:intron 35.0%
TTACAATTGTACACTCCAAC+GGG + Chr3:87451637-87451656 None:intergenic 35.0%
TTGGAAAAAATGGCAAGTGT+GGG + Chr3:87454122-87454141 None:intergenic 35.0%
TTGTTGGCACAAGATTGAAA+TGG + Chr3:87450077-87450096 None:intergenic 35.0%
! AGATATAAAAGAGTGCAAGG+AGG + Chr3:87449866-87449885 None:intergenic 35.0%
! GAAAAGAAACCCTTTAGAAG+AGG + Chr3:87449946-87449965 None:intergenic 35.0%
! TAGGTTTGTTCAAATCACGT+TGG - Chr3:87453765-87453784 MsG0380016754.01.T01:intron 35.0%
! TTGATCTATGGTAGTGAATC+TGG + Chr3:87450278-87450297 None:intergenic 35.0%
!! AGTAAAGTGGGTTTTCATAG+TGG + Chr3:87451037-87451056 None:intergenic 35.0%
!! GAAAGTGGGTTTTAGTAAAG+TGG + Chr3:87451050-87451069 None:intergenic 35.0%
!! TTCTAAAGGGTTTCTTTTCG+AGG - Chr3:87449947-87449966 MsG0380016754.01.T01:CDS 35.0%
!!! ATTTGGTTTTTCTAGCAGGA+TGG - Chr3:87454009-87454028 MsG0380016754.01.T01:intron 35.0%
AATACTAGCAGCATGTTGGT+TGG + Chr3:87453482-87453501 None:intergenic 40.0%
GAAATGCTACAGTGGCTAAT+TGG + Chr3:87454141-87454160 None:intergenic 40.0%
GAATACTTGGCTAGGTAAAG+AGG - Chr3:87450695-87450714 MsG0380016754.01.T01:intron 40.0%
GATAAAGCATATACTCCCTC+CGG - Chr3:87450453-87450472 MsG0380016754.01.T01:intron 40.0%
GATTTGATGAAGAATCCGAC+GGG + Chr3:87454184-87454203 None:intergenic 40.0%
GGAGTGTACAATTGTAACAC+GGG - Chr3:87451640-87451659 MsG0380016754.01.T01:intron 40.0%
TAATAAAACTTCGGCAGTCG+CGG + Chr3:87452206-87452225 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+GGG + Chr3:87450471-87450490 None:intergenic 40.0%
TTGCTTATAATAGTGACCCG+AGG - Chr3:87450584-87450603 MsG0380016754.01.T01:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG + Chr3:87450472-87450491 None:intergenic 40.0%
! AAGAAACCCTTTAGAAGAGG+CGG + Chr3:87449943-87449962 None:intergenic 40.0%
! AGCATGTTGGTTGGATAAGA+GGG + Chr3:87453473-87453492 None:intergenic 40.0%
! GTGTAATATTGCGGTGTTGA+GGG + Chr3:87450013-87450032 None:intergenic 40.0%
! TGTGTAATATTGCGGTGTTG+AGG + Chr3:87450014-87450033 None:intergenic 40.0%
!! GTTGAGGGTTAGTTGAAACA+GGG + Chr3:87449998-87450017 None:intergenic 40.0%
!! TGTGAGATAGTGGGTTCTAA+AGG - Chr3:87453357-87453376 MsG0380016754.01.T01:intron 40.0%
!! TGTTGAGGGTTAGTTGAAAC+AGG + Chr3:87449999-87450018 None:intergenic 40.0%
!! TTGAGGGTTAGTTGAAACAG+GGG + Chr3:87449997-87450016 None:intergenic 40.0%
!!! GGCTTTTAGAATACTTGGCT+AGG - Chr3:87450687-87450706 MsG0380016754.01.T01:intron 40.0%
ACACCGCAATATTACACAGC+AGG - Chr3:87450016-87450035 MsG0380016754.01.T01:CDS 45.0%
ACTACTACAGCTGTTGCAGT+CGG - Chr3:87450175-87450194 MsG0380016754.01.T01:intron 45.0%
AGATTCCGGCAAGTTAGAGA+CGG + Chr3:87449916-87449935 None:intergenic 45.0%
! CAGCATGTTGGTTGGATAAG+AGG + Chr3:87453474-87453493 None:intergenic 45.0%
! GGACAGAACCTAAGTATTGC+AGG - Chr3:87454030-87454049 MsG0380016754.01.T01:CDS 45.0%
!!! GTTTTGGTCTTGATTCCCGT+CGG - Chr3:87454166-87454185 MsG0380016754.01.T01:CDS 45.0%
!! ATATATTAATAATATATATG+TGG - Chr3:87451756-87451775 MsG0380016754.01.T01:intron 5.0%
!! TATATTAATAATATATATGT+GGG - Chr3:87451757-87451776 MsG0380016754.01.T01:intron 5.0%
!! TTAATATATTAATAAAACTT+AGG + Chr3:87451745-87451764 None:intergenic 5.0%
AGAGTGCAAGGAGGAGAAAG+AGG + Chr3:87449857-87449876 None:intergenic 50.0%
AGATACCCGTGCTACAGAAC+GGG + Chr3:87453288-87453307 None:intergenic 50.0%
CTTCCTCCGCCTCTTCTAAA+GGG - Chr3:87449934-87449953 MsG0380016754.01.T01:CDS 50.0%
GAGATACCCGTGCTACAGAA+CGG + Chr3:87453289-87453308 None:intergenic 50.0%
TCTTCCTCCGCCTCTTCTAA+AGG - Chr3:87449933-87449952 MsG0380016754.01.T01:CDS 50.0%
! AAACCCTTTAGAAGAGGCGG+AGG + Chr3:87449940-87449959 None:intergenic 50.0%
GAAGAGGCGGAGGAAGATTC+CGG + Chr3:87449930-87449949 None:intergenic 55.0%
TTCCGGCAAGTTAGAGACGG+TGG + Chr3:87449913-87449932 None:intergenic 55.0%
AGGATGTGGAGCCACGACAC+TGG - Chr3:87453447-87453466 MsG0380016754.01.T01:intron 60.0%
CGCTACCCGTTCTGTAGCAC+GGG - Chr3:87453280-87453299 MsG0380016754.01.T01:intron 60.0%
CGGCAAGTTAGAGACGGTGG+CGG + Chr3:87449910-87449929 None:intergenic 60.0%
GCGCTACCCGTTCTGTAGCA+CGG - Chr3:87453279-87453298 MsG0380016754.01.T01:intron 60.0%
GGATAAGAGGGCCAGTGTCG+TGG + Chr3:87453461-87453480 None:intergenic 60.0%
GTGCTACAGAACGGGTAGCG+CGG + Chr3:87453280-87453299 None:intergenic 60.0%
TGTTGCGTCTCGACTGCGCT+TGG + Chr3:87453235-87453254 None:intergenic 60.0%
! CGCCACCGTCTCTAACTTGC+CGG - Chr3:87449908-87449927 MsG0380016754.01.T01:CDS 60.0%
AGTCGAGACGCAACACGCGG+CGG - Chr3:87453240-87453259 MsG0380016754.01.T01:intron 65.0%
CGCAGTCGAGACGCAACACG+CGG - Chr3:87453237-87453256 MsG0380016754.01.T01:intron 65.0%
CAGAACGGGTAGCGCGGCGA+AGG + Chr3:87453274-87453293 None:intergenic 70.0%
! CGCAACACGCGGCGGAGCCG+CGG - Chr3:87453248-87453267 MsG0380016754.01.T01:intron 80.0%
! GGGTAGCGCGGCGAAGGCCG+CGG + Chr3:87453268-87453287 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr3 gene 87449789 87454278 87449789 ID=MsG0380016754.01;Name=MsG0380016754.01
Chr3 mRNA 87449789 87454278 87449789 ID=MsG0380016754.01.T01;Parent=MsG0380016754.01;Name=MsG0380016754.01.T01;_AED=0.50;_eAED=0.60;_QI=0|0|0|1|0|0|2|0|167
Chr3 exon 87454030 87454278 87454030 ID=MsG0380016754.01.T01:exon:22158;Parent=MsG0380016754.01.T01
Chr3 exon 87449789 87450043 87449789 ID=MsG0380016754.01.T01:exon:22157;Parent=MsG0380016754.01.T01
Chr3 CDS 87454030 87454278 87454030 ID=MsG0380016754.01.T01:cds;Parent=MsG0380016754.01.T01
Chr3 CDS 87449789 87450043 87449789 ID=MsG0380016754.01.T01:cds;Parent=MsG0380016754.01.T01
Gene Sequence

>MsG0380016754.01.T01

ATGTTAGACGAGTTCATTACAGAAAGCTATAGAATTCCATGGTTGATTTGGATTCAACTAATTGTCCTCTTTCTCCTCCTTGCACTCTTTTATATCTTCATCTTCGACGACGATGACGCCGCCACCGTCTCTAACTTGCCGGAATCTTCCTCCGCCTCTTCTAAAGGGTTTCTTTTCGAGGAGATTCAACAGATTGACTCAATTGCCCCTGTTTCAACTAACCCTCAACACCGCAATATTACACAGCAGCAGGATGGACAGAACCTAAGTATTGCAGGTGAAATAGCAACATGTTCAAACATGAGAAGTGGAAGATTAATGAGGGGAGTCTTCAACACTGAGTTCCCACACTTGCCATTTTTTCCAATTAGCCACTGTAGCATTTCTCAATGTTTTGGTCTTGATTCCCGTCGGATTCTTCATCAAATCAGAAATGTAGAAAAAGGAAAGAAAGTTGAGTGTGAAAATTATGCTCTTGTAGATTATTGTAAATGTCATTCGTGA
Protein sequence

>MsG0380016754.01.T01

MLDEFITESYRIPWLIWIQLIVLFLLLALFYIFIFDDDDAATVSNLPESSSASSKGFLFEEIQQIDSIAPVSTNPQHRNITQQQDGQNLSIAGEIATCSNMRSGRLMRGVFNTEFPHLPFFPISHCSISQCFGLDSRRILHQIRNVEKGKKVECENYALVDYCKCHS*