AlfalfaGEDB | MsG0380016924.01

M. sativa cultivar ZhongmuNo.1 / MsG0380016924.01

KEGG: -
GO: GO:0005524
PFAM: PF00004 PF10431

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016924.01.T01	XP_039687659.1	82.102	352	14	3	1	303	122	473	0	556
MsG0380016924.01.T01	XP_039687659.1	95.181	83	4	0	402	484	856	938	7.56E-40	168
MsG0380016924.01.T01	XP_039687659.1	97.436	78	2	0	324	401	622	699	1.20E-36	158

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016924.01.T01	P42762	56.125	351	101	6	1	303	112	457	1.73E-101	332

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016924.01.T01	G7J2Q6	82.102	352	14	3	1	303	133	484	0.0	556

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280008139.01	MsG0380016924.01	0.821233	4.502853e-53	3.642499e-50

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016924.01.T01	MTR_3g098310	82.102	352	14	3	1	303	133	484	0.0	556
MsG0380016924.01.T01	MTR_3g098310	95.181	83	4	0	402	484	867	949	9.51e-44	168
MsG0380016924.01.T01	MTR_3g098310	97.436	78	2	0	324	401	633	710	1.57e-40	158
MsG0380016924.01.T01	MTR_3g098310	82.102	352	14	3	1	303	133	484	0.0	555
MsG0380016924.01.T01	MTR_3g098310	97.436	78	2	0	324	401	633	710	1.29e-40	158
MsG0380016924.01.T01	MTR_7g012820	47.248	218	105	3	90	302	141	353	1.88e-51	190
MsG0380016924.01.T01	MTR_7g012820	52.439	82	36	1	320	401	562	640	1.10e-18	90.9
MsG0380016924.01.T01	MTR_7g012820	47.248	218	105	3	90	302	212	424	2.10e-51	191
MsG0380016924.01.T01	MTR_7g012820	52.439	82	36	1	320	401	633	711	1.20e-18	90.9
MsG0380016924.01.T01	MTR_6g013660	49.754	203	92	3	105	302	223	420	5.09e-51	189

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016924.01.T01	AT5G51070	56.125	351	101	6	1	303	112	457	1.77e-102	332
MsG0380016924.01.T01	AT5G51070	74.359	78	20	0	324	401	607	684	3.13e-30	127
MsG0380016924.01.T01	AT5G51070	47.872	94	48	1	402	494	842	935	2.01e-20	96.3
MsG0380016924.01.T01	AT5G15450	48.768	203	94	3	105	302	221	418	4.66e-50	187
MsG0380016924.01.T01	AT5G15450	53.659	82	35	1	320	401	627	705	4.41e-20	95.5
MsG0380016924.01.T01	AT5G50920	50.000	192	87	2	115	302	251	437	2.83e-49	184
MsG0380016924.01.T01	AT5G50920	57.692	78	33	0	324	401	588	665	5.15e-23	104
MsG0380016924.01.T01	AT5G50920	48.148	81	41	1	404	483	824	904	4.25e-16	82.8
MsG0380016924.01.T01	AT3G48870	50.526	190	85	2	117	302	243	427	1.20e-48	182
MsG0380016924.01.T01	AT3G48870	60.256	78	31	0	324	401	578	655	8.64e-24	107

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Find 147 sgRNAs with CRISPR-Local

Find 539 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGAGAGGAGAGCTAG+AGG	0.545345	3:-89452969	MsG0380016924.01.T01:CDS
AAAAGCAATCCAATTCTTCT+GGG	0.464852	3:-89453263	MsG0380016924.01.T01:CDS
AAAAGGATAAGGCTTTGGCA+CGG	0.663550	3:-89451752	MsG0380016924.01.T01:CDS
AAAATCAATTCCTCGGGGCA+TGG	0.547293	3:+89449307	None:intergenic
AAACCGAAATCAATTGCTGA+TGG	0.597725	3:-89446951	MsG0380016924.01.T01:CDS
AAACTAAGGGAACGTGTGAT+TGG	0.431441	3:-89450678	MsG0380016924.01.T01:CDS
AAACTTAATTGCAAACCCAT+TGG	0.514028	3:-89449198	MsG0380016924.01.T01:CDS
AAAGTTGCTATGGGTCTACC+GGG	0.647341	3:+89450591	None:intergenic
AACAGCTATTGCAGAGGGAC+TGG	0.445401	3:-89453222	MsG0380016924.01.T01:CDS
AACTGAACTAGCAAAGTCTT+TGG	0.432809	3:-89450538	MsG0380016924.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGCAATCCAATTCTTC+TGG	-	Chr3:89448752-89448771	MsG0380016924.01.T01:intron	30.0%
AAAAGCAATCCAATTCTTCT+GGG	-	Chr3:89448753-89448772	MsG0380016924.01.T01:intron	30.0%
AAACTAGTTATGCAACTCAA+AGG	+	Chr3:89448093-89448112	None:intergenic	30.0%
AAACTTAATTGCAAACCCAT+TGG	-	Chr3:89452818-89452837	MsG0380016924.01.T01:intron	30.0%
AAGAAGAAGAAGAAGAAATC+CGG	-	Chr3:89455117-89455136	MsG0380016924.01.T01:CDS	30.0%
AATCTTAAGTGTGCATTTGA+TGG	+	Chr3:89453186-89453205	None:intergenic	30.0%
ACAAAAATCTTCACCTTTGA+TGG	-	Chr3:89453624-89453643	MsG0380016924.01.T01:CDS	30.0%
ACAATACTCCAAATACCAAA+TGG	+	Chr3:89447233-89447252	None:intergenic	30.0%
ACAGAATAGTAAAGTTGCTA+TGG	+	Chr3:89451438-89451457	None:intergenic	30.0%
ACCAAACAAACCAATAAACT+TGG	+	Chr3:89450564-89450583	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	89446881	89455157	89446881	ID=MsG0380016924.01;Name=MsG0380016924.01
Chr3	mRNA	89446881	89455157	89446881	ID=MsG0380016924.01.T01;Parent=MsG0380016924.01;Name=MsG0380016924.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|0.22\|0.2\|1\|0.88\|1\|10\|0\|591
Chr3	exon	89454946	89455157	89454946	ID=MsG0380016924.01.T01:exon:12324;Parent=MsG0380016924.01.T01
Chr3	exon	89453531	89453664	89453531	ID=MsG0380016924.01.T01:exon:12323;Parent=MsG0380016924.01.T01
Chr3	exon	89453181	89453413	89453181	ID=MsG0380016924.01.T01:exon:12322;Parent=MsG0380016924.01.T01
Chr3	exon	89452930	89453038	89452930	ID=MsG0380016924.01.T01:exon:12321;Parent=MsG0380016924.01.T01
Chr3	exon	89452711	89452829	89452711	ID=MsG0380016924.01.T01:exon:12320;Parent=MsG0380016924.01.T01
Chr3	exon	89451663	89451817	89451663	ID=MsG0380016924.01.T01:exon:12319;Parent=MsG0380016924.01.T01
Chr3	exon	89450518	89450758	89450518	ID=MsG0380016924.01.T01:exon:12318;Parent=MsG0380016924.01.T01
Chr3	exon	89449132	89449372	89449132	ID=MsG0380016924.01.T01:exon:12317;Parent=MsG0380016924.01.T01
Chr3	exon	89447115	89447265	89447115	ID=MsG0380016924.01.T01:exon:12316;Parent=MsG0380016924.01.T01
Chr3	exon	89446881	89447061	89446881	ID=MsG0380016924.01.T01:exon:12315;Parent=MsG0380016924.01.T01
Chr3	CDS	89454946	89455157	89454946	ID=MsG0380016924.01.T01:cds;Parent=MsG0380016924.01.T01
Chr3	CDS	89453531	89453664	89453531	ID=MsG0380016924.01.T01:cds;Parent=MsG0380016924.01.T01
Chr3	CDS	89453181	89453413	89453181	ID=MsG0380016924.01.T01:cds;Parent=MsG0380016924.01.T01
Chr3	CDS	89452930	89453038	89452930	ID=MsG0380016924.01.T01:cds;Parent=MsG0380016924.01.T01
Chr3	CDS	89452711	89452829	89452711	ID=MsG0380016924.01.T01:cds;Parent=MsG0380016924.01.T01
Chr3	CDS	89451663	89451817	89451663	ID=MsG0380016924.01.T01:cds;Parent=MsG0380016924.01.T01
Chr3	CDS	89450518	89450758	89450518	ID=MsG0380016924.01.T01:cds;Parent=MsG0380016924.01.T01
Chr3	CDS	89449132	89449372	89449132	ID=MsG0380016924.01.T01:cds;Parent=MsG0380016924.01.T01
Chr3	CDS	89447115	89447265	89447115	ID=MsG0380016924.01.T01:cds;Parent=MsG0380016924.01.T01
Chr3	CDS	89446881	89447061	89446881	ID=MsG0380016924.01.T01:cds;Parent=MsG0380016924.01.T01

Gene Sequence

>MsG0380016924.01.T01

ATGCTTGGTCTCATAGCTGAAGCTGAAGAGACAAAACGTTCTCAAAACGGTTTTCTTGATTCTGGTGTAGTCACTCTTGAGAAAGCTCGTGATGCTGTTCCTCATCTCAATGATTCAACCAACTACGTTGATGATAATGGAGTGTATGTGTATGAAGATCGACCTGTTCCGTTTTCTTTTGGTACTAAACGTGTTTTTGAAGCTGCTGTTGAACTCGGGTCAAACCCAGATCAGTTGGCAGCTGTGGCATATTCAAGACTACAAAAAGAGCTGGCTAGAGATGGTAGAGAGCCAGGTTATGTGTCCAATGGGGGACACAACAAATCTATATCACAAAGGCGTTCTGATAAAAAGAGTGCTCTATCACAATTCTGCGTGGATCTCACTGCCCGTGCAAGTGTTGGGCTGATTGATCCAGTTATTGGCCGAGAAGTTGAAGTTCAAAGAATTATTCAAATACTATGCCGAAAGACAAAAAGCAATCCAATTCTTCTGGGTGAAGCTGGAGTTGGGAAAACAGCTATTGCAGAGGGACTGGCAATTCTTATTTCTCGGGCAGAAGTTGCTCCTTTTCTATTGACAAAACGTGTCATGTCCTTGGATGTAGGACTATTAATGGCTGGGGCAAAAGAGAGAGGAGAGCTAGAGGACCGCGTTACAAAATTAATAAAAGATATAATAGAGTCAGGTGATGTCATTCTTTTCATTGACGAAGTTCACACACTTGTTCAATCAGGGACAACCGGGAGGGGAAATAAGGGTTCTGGCTTTGACATTGCTAATTGCTTAAACCTTCACTTGGAAGGGTGTATTGCATCTACCACTATTGATGAATACAGGCTTCATTTTGAAAAGGATAAGGCTTTGGCACGGCGGTTTCAACCTGTTTGGATTGATGAGCCGAGTGAGGTTGCACTTTATATTCATGTCTATACTGTTCAAGTGACTCCCTCTTCTCCTTTGAATTCCATTCAGCAGCTCACTGCTGATGAAAGAAGTCTTCTGTTAGATCTGGATAATAAACTAAGGGAACGTGTGATTGGACAAGAGGAGGCTGTTTCTGCCATTTCTAGATCTGTGAAGAGATCTCGAGTCGGCCTTCGGGATCCCGGTAGACCCATAGCAACTTTACTATTCTGTGGCCCAACTGGCGTTGGGAAAACTGAACTAGCAAAGTCTTTGGCTGCATGCTATTTTGGATCGTTGCTCCAAATATTTGATTTGCTGCTGCAAGACGTGAAAAGGACCATGCCCCGAGGAATTGATTTTGAGGTGTCTGAATCAGTCAAGGACCTTGTCTGTAAGGAAGGCTATGACCCAACATATGGTGCTAGACCTCTCAGGAGAGCCATTGTAAACTTAATTGCAAACCCATTGGCTGAAGCATTGCTTGCTGAAAAGTGCAAGGAAGGTGACACTGTCTTCATTGATTTGGATGCCAATGAGAACAGCGACAACATGGAAGAAAATAACCACTCCACAGCCTCCGACGCTCCAATTCCGCCGGAAAACCAACACGACGCCGGCGCCAATATTTCGAAATCGGTGAATGAACTCAACGATTTGTCAATTTCAATTCAAGCTTTCAAAAACAGAACCAAAGAACTCGAAGCCCTAGGCACCACCACCGCCGCAAATGTGGTCACCGATAACGGTAACATTGTTCCATCTGAACCAGAATCGAAACCGAAATCAATTGCTGATGGCGAAGAAATAGAAGAAGAAGATCCTGAAGAAGAAGAAGAAGAAGAAATCCGGAAGAAGAAGAGAGAGTAG

Protein sequence

>MsG0380016924.01.T01

MLGLIAEAEETKRSQNGFLDSGVVTLEKARDAVPHLNDSTNYVDDNGVYVYEDRPVPFSFGTKRVFEAAVELGSNPDQLAAVAYSRLQKELARDGREPGYVSNGGHNKSISQRRSDKKSALSQFCVDLTARASVGLIDPVIGREVEVQRIIQILCRKTKSNPILLGEAGVGKTAIAEGLAILISRAEVAPFLLTKRVMSLDVGLLMAGAKERGELEDRVTKLIKDIIESGDVILFIDEVHTLVQSGTTGRGNKGSGFDIANCLNLHLEGCIASTTIDEYRLHFEKDKALARRFQPVWIDEPSEVALYIHVYTVQVTPSSPLNSIQQLTADERSLLLDLDNKLRERVIGQEEAVSAISRSVKRSRVGLRDPGRPIATLLFCGPTGVGKTELAKSLAACYFGSLLQIFDLLLQDVKRTMPRGIDFEVSESVKDLVCKEGYDPTYGARPLRRAIVNLIANPLAEALLAEKCKEGDTVFIDLDANENSDNMEENNHSTASDAPIPPENQHDAGANISKSVNELNDLSISIQAFKNRTKELEALGTTTAANVVTDNGNIVPSEPESKPKSIADGEEIEEEDPEEEEEEEIRKKKRE*