Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017389.01.T01 | XP_003603340.2 | 93.976 | 83 | 1 | 1 | 1 | 83 | 497 | 575 | 3.99E-42 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017389.01.T01 | G7JAF2 | 93.976 | 83 | 1 | 1 | 1 | 83 | 497 | 575 | 1.90e-42 | 154 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000817.01 | MsG0380017389.01 | 0.804580 | 2.018152e-49 | 1.044365e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017389.01.T01 | MTR_3g106510 | 93.976 | 83 | 1 | 1 | 1 | 83 | 497 | 575 | 4.83e-46 | 154 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGTTCTCCTTGATCTTT+TGG | 0.146015 | 3:+95731045 | MsG0380017389.01.T01:CDS |
| CTTGTCACCTTTCAGGGATA+TGG | 0.218444 | 3:-95731269 | None:intergenic |
| TTGTCACCTTTCAGGGATAT+GGG | 0.414216 | 3:-95731268 | None:intergenic |
| CGTCTTGTTGGTGCAGGTGC+AGG | 0.435124 | 3:-95731176 | None:intergenic |
| AGTTCAAGTGCATGACTTGA+TGG | 0.451478 | 3:-95731242 | None:intergenic |
| GGTGTAGGTGCACGTCTTGT+TGG | 0.466169 | 3:-95731155 | None:intergenic |
| GGTGTAAGTGCAAGTCTTGT+TGG | 0.470877 | 3:-95731221 | None:intergenic |
| AGTGCAGGTGCACGTCTTGT+TGG | 0.512032 | 3:-95731188 | None:intergenic |
| GGTGCACGTCTTGTTGGTGC+AGG | 0.521105 | 3:-95731182 | None:intergenic |
| GTTGGTGCAGGTGCAAGTGC+AGG | 0.524450 | 3:-95731203 | None:intergenic |
| GTTGGTGCAGGTGCAGGTGT+AGG | 0.529964 | 3:-95731170 | None:intergenic |
| AGTGCAAGTCTTGTTGGTGC+AGG | 0.565751 | 3:-95731215 | None:intergenic |
| GGTGCACGTCTTGTTGGTGC+AGG | 0.574585 | 3:-95731149 | None:intergenic |
| ATACACACCCCAAAAGATCA+AGG | 0.584372 | 3:-95731054 | None:intergenic |
| GTTGGTGCAGGAGCTCGTGT+AGG | 0.611574 | 3:-95731137 | None:intergenic |
| GGGGTGTGTATGGAAGAGAG+CGG | 0.642171 | 3:+95731066 | MsG0380017389.01.T01:CDS |
| GTGTGTATGGAAGAGAGCGG+AGG | 0.703717 | 3:+95731069 | MsG0380017389.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGTTCTCCTTGATCTTTTG+GGG | + | Chr3:95731047-95731066 | MsG0380017389.01.T01:CDS | 35.0% |
| ! | GATGTTCTCCTTGATCTTTT+GGG | + | Chr3:95731046-95731065 | MsG0380017389.01.T01:CDS | 35.0% |
| ! | TGATGTTCTCCTTGATCTTT+TGG | + | Chr3:95731045-95731064 | MsG0380017389.01.T01:CDS | 35.0% |
| AGTTCAAGTGCATGACTTGA+TGG | - | Chr3:95731245-95731264 | None:intergenic | 40.0% | |
| ATACACACCCCAAAAGATCA+AGG | - | Chr3:95731057-95731076 | None:intergenic | 40.0% | |
| !!! | TTGATCTTTTGGGGTGTGTA+TGG | + | Chr3:95731056-95731075 | MsG0380017389.01.T01:CDS | 40.0% |
| ! | GGTGTAAGTGCAAGTCTTGT+TGG | - | Chr3:95731224-95731243 | None:intergenic | 45.0% |
| !! | AGTGCAAGTCTTGTTGGTGC+AGG | - | Chr3:95731218-95731237 | None:intergenic | 50.0% |
| AGTGCAGGTGCACGTCTTGT+TGG | - | Chr3:95731191-95731210 | None:intergenic | 55.0% | |
| GGGGTGTGTATGGAAGAGAG+CGG | + | Chr3:95731066-95731085 | MsG0380017389.01.T01:CDS | 55.0% | |
| GGTGTAGGTGCACGTCTTGT+TGG | - | Chr3:95731158-95731177 | None:intergenic | 55.0% | |
| GTGTGTATGGAAGAGAGCGG+AGG | + | Chr3:95731069-95731088 | MsG0380017389.01.T01:CDS | 55.0% | |
| GTTGGTGCAGGAGCTCGTGT+AGG | - | Chr3:95731140-95731159 | None:intergenic | 60.0% | |
| GTTGGTGCAGGTGCAAGTGC+AGG | - | Chr3:95731206-95731225 | None:intergenic | 60.0% | |
| GTTGGTGCAGGTGCAGGTGT+AGG | - | Chr3:95731173-95731192 | None:intergenic | 60.0% | |
| ! | CGTCTTGTTGGTGCAGGTGC+AGG | - | Chr3:95731179-95731198 | None:intergenic | 60.0% |
| ! | GGTGCACGTCTTGTTGGTGC+AGG | - | Chr3:95731152-95731171 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 95731029 | 95731280 | 95731029 | ID=MsG0380017389.01;Name=MsG0380017389.01 |
| Chr3 | mRNA | 95731029 | 95731280 | 95731029 | ID=MsG0380017389.01.T01;Parent=MsG0380017389.01;Name=MsG0380017389.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|83 |
| Chr3 | exon | 95731029 | 95731280 | 95731029 | ID=MsG0380017389.01.T01:exon:1740;Parent=MsG0380017389.01.T01 |
| Chr3 | CDS | 95731029 | 95731280 | 95731029 | ID=MsG0380017389.01.T01:cds;Parent=MsG0380017389.01.T01 |
| Gene Sequence |
| Protein sequence |
