Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017867.01.T01 | XP_003625817.1 | 96.154 | 52 | 2 | 0 | 1 | 52 | 21 | 72 | 2.09E-27 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017867.01.T01 | Q9LXJ2 | 88.462 | 52 | 6 | 0 | 1 | 52 | 21 | 72 | 1.18E-27 | 96.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017867.01.T01 | G7L638 | 96.154 | 52 | 2 | 0 | 1 | 52 | 21 | 72 | 9.99e-28 | 103 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380017867.01 | MsG0480021159.01 | 0.803171 | 3.959157e-49 | 1.975994e-46 |
| MsG0380017867.01 | MsG0780040511.01 | 0.810976 | 8.819238e-51 | 5.393553e-48 |
| MsG0380017867.01 | MsG0880043770.01 | 0.801391 | 9.204936e-49 | 4.390686e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017867.01.T01 | MTR_7g104570 | 96.154 | 52 | 2 | 0 | 1 | 52 | 21 | 72 | 2.53e-31 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017867.01.T01 | AT3G52730 | 88.462 | 52 | 6 | 0 | 1 | 52 | 21 | 72 | 1.20e-28 | 96.7 |
| MsG0380017867.01.T01 | AT3G52730 | 91.667 | 36 | 3 | 0 | 1 | 36 | 21 | 56 | 1.00e-18 | 72.0 |
Find 9 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGAACGGGCTGTGGATTA+CGG | 0.374620 | 3:-101651922 | MsG0380017867.01.T01:CDS |
| ATTACGGTGTTCACAAGCTC+TGG | 0.424543 | 3:-101651906 | MsG0380017867.01.T01:CDS |
| CTCTGGGAAATAAATAATGT+TGG | 0.450240 | 3:-101651889 | MsG0380017867.01.T01:CDS |
| TCTGGGAAATAAATAATGTT+GGG | 0.469918 | 3:-101651888 | MsG0380017867.01.T01:CDS |
| TATGTTACCTTCGTCATCGC+CGG | 0.481783 | 3:-101651955 | MsG0380017867.01.T01:CDS |
| TTACGGTGTTCACAAGCTCT+GGG | 0.588806 | 3:-101651905 | MsG0380017867.01.T01:CDS |
| CCGTTCACCGAGAAAAGCAC+CGG | 0.611695 | 3:+101651936 | None:intergenic |
| AAAAGCACCGGCGATGACGA+AGG | 0.627325 | 3:+101651948 | None:intergenic |
| AGGTAACATAGACAGAGTTG+CGG | 0.713031 | 3:+101651968 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTGGGAAATAAATAATGTT+GGG | - | Chr3:101651922-101651941 | MsG0380017867.01.T01:CDS | 25.0% |
| ! | ATATGAAGACATTTTTGTGC+TGG | - | Chr3:101651950-101651969 | MsG0380017867.01.T01:CDS | 30.0% |
| ! | CTCTGGGAAATAAATAATGT+TGG | - | Chr3:101651921-101651940 | MsG0380017867.01.T01:CDS | 30.0% |
| ! | TATGAAGACATTTTTGTGCT+GGG | - | Chr3:101651951-101651970 | MsG0380017867.01.T01:CDS | 30.0% |
| ! | ATGAAGACATTTTTGTGCTG+GGG | - | Chr3:101651952-101651971 | MsG0380017867.01.T01:CDS | 35.0% |
| AGGTAACATAGACAGAGTTG+CGG | + | Chr3:101651845-101651864 | None:intergenic | 40.0% | |
| ATTACGGTGTTCACAAGCTC+TGG | - | Chr3:101651904-101651923 | MsG0380017867.01.T01:CDS | 45.0% | |
| TATGTTACCTTCGTCATCGC+CGG | - | Chr3:101651855-101651874 | MsG0380017867.01.T01:CDS | 45.0% | |
| TTACGGTGTTCACAAGCTCT+GGG | - | Chr3:101651905-101651924 | MsG0380017867.01.T01:CDS | 45.0% | |
| ! | ACATTTTTGTGCTGGGGCAA+AGG | - | Chr3:101651958-101651977 | MsG0380017867.01.T01:CDS | 45.0% |
| ! | TTTTGTGCTGGGGCAAAGGT+CGG | - | Chr3:101651962-101651981 | MsG0380017867.01.T01:CDS | 50.0% |
| ! | CCGGTGCTTTTCTCGGTGAA+CGG | - | Chr3:101651874-101651893 | MsG0380017867.01.T01:CDS | 55.0% |
| ! | CCGTTCACCGAGAAAAGCAC+CGG | + | Chr3:101651877-101651896 | None:intergenic | 55.0% |
| ! | CGGTGCTTTTCTCGGTGAAC+GGG | - | Chr3:101651875-101651894 | MsG0380017867.01.T01:CDS | 55.0% |
| ! | GTCATCGCCGGTGCTTTTCT+CGG | - | Chr3:101651867-101651886 | MsG0380017867.01.T01:CDS | 55.0% |
| !! | AAAAGCACCGGCGATGACGA+AGG | + | Chr3:101651865-101651884 | None:intergenic | 55.0% |
| !! | GGTGAACGGGCTGTGGATTA+CGG | - | Chr3:101651888-101651907 | MsG0380017867.01.T01:CDS | 55.0% |
| !!! | TTTTCTCGGTGAACGGGCTG+TGG | - | Chr3:101651881-101651900 | MsG0380017867.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 101651837 | 101651995 | 101651837 | ID=MsG0380017867.01;Name=MsG0380017867.01 |
| Chr3 | mRNA | 101651837 | 101651995 | 101651837 | ID=MsG0380017867.01.T01;Parent=MsG0380017867.01;Name=MsG0380017867.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|52 |
| Chr3 | exon | 101651837 | 101651995 | 101651837 | ID=MsG0380017867.01.T01:exon:2541;Parent=MsG0380017867.01.T01 |
| Chr3 | CDS | 101651837 | 101651995 | 101651837 | ID=MsG0380017867.01.T01:cds;Parent=MsG0380017867.01.T01 |
| Gene Sequence |
| Protein sequence |
