AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018380.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018380.01.T01 MTR_4g010830 90.417 480 45 1 2 481 423 901 0.0 852
MsG0480018380.01.T01 MTR_5g013420 85.062 482 69 3 1 481 531 1010 0.0 817
MsG0480018380.01.T01 MTR_5g013420 85.062 482 69 3 1 481 531 1010 0.0 817
MsG0480018380.01.T01 MTR_4g010550 82.254 417 37 7 70 481 1 385 0.0 631
MsG0480018380.01.T01 MTR_8g042750 40.716 447 245 10 8 438 122 564 1.29e-100 317
MsG0480018380.01.T01 MTR_7g088370 42.963 405 205 12 51 438 202 597 3.12e-98 311
MsG0480018380.01.T01 MTR_3g011440 41.706 422 229 10 32 438 121 540 3.46e-98 310
MsG0480018380.01.T01 MTR_8g070070 41.404 413 219 12 88 481 299 707 8.71e-93 298
MsG0480018380.01.T01 MTR_8g070075 40.436 413 227 10 86 481 290 700 3.70e-91 293
MsG0480018380.01.T01 MTR_1g035420 35.968 506 273 14 12 481 106 596 1.08e-86 280
MsG0480018380.01.T01 MTR_8g071030 40.633 411 223 10 89 481 280 687 3.21e-86 280
MsG0480018380.01.T01 MTR_6g061270 35.363 509 254 14 41 481 114 615 4.02e-80 263
MsG0480018380.01.T01 MTR_7g445710 38.242 455 194 11 47 481 77 464 5.01e-80 259
MsG0480018380.01.T01 MTR_7g445530 38.022 455 195 11 47 481 77 464 1.27e-79 257
MsG0480018380.01.T01 MTR_7g084090 37.868 441 233 12 74 481 246 678 1.42e-79 263
MsG0480018380.01.T01 MTR_7g084090 40.052 387 208 10 74 439 246 629 1.13e-78 258
MsG0480018380.01.T01 MTR_1g048950 40.723 415 221 8 50 440 32 445 8.67e-78 253
MsG0480018380.01.T01 MTR_6g044690 40.591 372 186 13 86 435 109 467 4.60e-74 244
MsG0480018380.01.T01 MTR_7g450730 37.136 447 187 9 41 481 68 426 5.02e-74 242
MsG0480018380.01.T01 MTR_6g061200 37.740 416 216 11 85 481 195 586 2.56e-72 241
MsG0480018380.01.T01 MTR_1g035400 37.981 416 217 17 52 437 68 472 2.58e-71 237
MsG0480018380.01.T01 MTR_0376s0030 35.731 417 222 13 52 435 62 465 4.21e-70 234
MsG0480018380.01.T01 MTR_6g043960 36.267 375 173 13 88 435 50 385 2.89e-59 202
MsG0480018380.01.T01 MTR_2g054460 33.252 409 198 10 52 435 9 367 1.77e-51 181
MsG0480018380.01.T01 MTR_7g052480 36.864 236 135 9 257 481 245 477 4.89e-32 129
MsG0480018380.01.T01 MTR_5g018850 34.286 245 103 9 278 481 1208 1435 1.66e-26 114
MsG0480018380.01.T01 MTR_7g445810 36.364 198 98 6 48 238 91 267 5.79e-24 101
MsG0480018380.01.T01 MTR_7g445600 37.805 164 85 3 48 211 74 220 8.32e-24 102
MsG0480018380.01.T01 MTR_7g445600 52.941 68 27 1 371 438 239 301 4.10e-15 77.0
MsG0480018380.01.T01 MTR_8g078490 38.732 142 65 4 346 481 1056 1181 2.85e-23 104
MsG0480018380.01.T01 MTR_4g132610 54.762 84 37 1 347 429 851 934 7.61e-22 99.8
MsG0480018380.01.T01 MTR_8g078505 38.028 142 66 4 345 481 343 467 5.14e-20 93.2
MsG0480018380.01.T01 MTR_1g080340 29.167 216 97 6 280 439 267 482 4.54e-17 84.3
MsG0480018380.01.T01 MTR_7g098390 26.531 245 104 7 274 449 380 617 6.83e-17 84.0
MsG0480018380.01.T01 MTR_1g069570 27.519 258 115 10 280 481 428 669 2.09e-16 82.4
MsG0480018380.01.T01 MTR_1g069570 27.519 258 115 10 280 481 428 669 2.52e-16 82.4
MsG0480018380.01.T01 MTR_5g098680 35.032 157 84 4 114 259 267 416 4.28e-16 81.6
MsG0480018380.01.T01 MTR_8g102580 34.395 157 85 4 114 259 261 410 2.01e-15 79.3
MsG0480018380.01.T01 MTR_1g064770 45.312 64 34 1 359 421 27 90 2.34e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018380.01.T01 AT2G22740 59.234 444 165 7 52 481 270 711 6.52e-169 496
MsG0480018380.01.T01 AT2G22740 59.234 444 165 7 52 481 270 711 6.52e-169 496
MsG0480018380.01.T01 AT2G35160 57.208 437 154 5 51 481 270 679 3.21e-163 480
MsG0480018380.01.T01 AT2G35160 57.208 437 154 5 51 481 306 715 4.09e-163 481
MsG0480018380.01.T01 AT2G35160 57.208 437 154 5 51 481 306 715 4.09e-163 481
MsG0480018380.01.T01 AT2G35160 57.208 437 154 5 51 481 306 715 4.09e-163 481
MsG0480018380.01.T01 AT2G35160 57.208 437 154 5 51 481 306 715 4.09e-163 481
MsG0480018380.01.T01 AT5G04940 42.651 415 219 12 41 444 146 552 1.96e-97 308
MsG0480018380.01.T01 AT5G04940 42.651 415 219 12 41 444 146 552 1.96e-97 308
MsG0480018380.01.T01 AT1G73100 39.035 456 250 12 40 481 148 589 1.43e-89 287
MsG0480018380.01.T01 AT4G13460 36.842 456 266 9 44 481 137 588 1.50e-86 279
MsG0480018380.01.T01 AT4G13460 36.842 456 266 9 44 481 137 588 1.50e-86 279
MsG0480018380.01.T01 AT2G33290 34.205 459 285 6 37 481 134 589 9.22e-83 269
MsG0480018380.01.T01 AT2G33290 34.205 459 285 6 37 481 134 589 9.22e-83 269
MsG0480018380.01.T01 AT2G33290 34.205 459 285 6 37 481 134 589 9.22e-83 269
MsG0480018380.01.T01 AT2G33290 34.205 459 285 6 37 481 134 589 9.22e-83 269
MsG0480018380.01.T01 AT5G13960 37.939 456 241 13 54 481 104 545 1.07e-80 263
MsG0480018380.01.T01 AT5G13960 40.299 402 209 11 108 481 13 411 6.62e-80 257
MsG0480018380.01.T01 AT2G24740 35.337 416 216 12 40 438 250 629 5.49e-67 229
MsG0480018380.01.T01 AT1G17770 37.356 348 194 9 109 438 226 567 9.75e-66 224
MsG0480018380.01.T01 AT5G47150 46.544 217 112 4 48 262 114 328 1.55e-50 175
MsG0480018380.01.T01 AT5G47160 44.167 240 125 6 18 254 174 407 7.71e-45 162
MsG0480018380.01.T01 AT5G47160 44.167 240 125 6 18 254 174 407 7.71e-45 162
MsG0480018380.01.T01 AT5G47160 44.167 240 125 6 18 254 174 407 7.71e-45 162
MsG0480018380.01.T01 AT2G05900 35.514 214 127 7 127 331 1 212 7.41e-29 116
MsG0480018380.01.T01 AT2G23740 33.333 243 114 8 270 481 1082 1307 1.49e-24 108
MsG0480018380.01.T01 AT2G23740 33.333 243 114 8 270 481 1082 1307 1.49e-24 108
MsG0480018380.01.T01 AT2G23740 33.333 243 114 8 270 481 1075 1300 1.60e-24 108
MsG0480018380.01.T01 AT2G23740 33.333 243 114 8 270 481 1082 1307 1.66e-24 108
MsG0480018380.01.T01 AT2G23740 33.333 243 114 8 270 481 1082 1307 1.66e-24 108
MsG0480018380.01.T01 AT2G23740 33.333 243 114 8 270 481 1082 1307 1.66e-24 108
MsG0480018380.01.T01 AT3G04380 28.571 231 107 7 251 429 121 345 6.07e-18 86.7
MsG0480018380.01.T01 AT3G04380 28.571 231 107 7 251 429 94 318 7.36e-18 86.3
MsG0480018380.01.T01 AT3G04380 28.571 231 107 7 251 429 121 345 8.75e-18 86.3
MsG0480018380.01.T01 AT3G04380 28.571 231 107 7 251 429 94 318 9.61e-18 85.9
MsG0480018380.01.T01 AT3G04380 28.571 231 107 7 251 429 105 329 9.77e-18 86.3
MsG0480018380.01.T01 AT3G04380 28.571 231 107 7 251 429 78 302 1.31e-17 85.5
MsG0480018380.01.T01 AT1G57820 34.177 158 85 5 114 259 277 427 4.46e-16 81.6
MsG0480018380.01.T01 AT1G57820 34.177 158 85 5 114 259 277 427 4.46e-16 81.6
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 416 619 1.21e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 416 619 1.21e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 396 599 1.33e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 396 599 1.33e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 373 576 1.39e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 373 576 1.39e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 373 576 1.39e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 373 576 1.39e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 393 596 1.42e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 393 596 1.42e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 393 596 1.42e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 393 596 1.42e-15 80.1
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 304 507 2.01e-15 79.3
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 324 527 2.26e-15 79.3
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 324 527 2.26e-15 79.3
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 324 527 2.26e-15 79.3
MsG0480018380.01.T01 AT5G43990 30.392 204 91 8 280 432 324 527 2.26e-15 79.3
MsG0480018380.01.T01 AT1G57820 33.548 155 87 5 114 259 277 424 2.56e-15 79.0
MsG0480018380.01.T01 AT1G66050 34.507 142 75 4 114 244 262 396 3.27e-14 75.5
MsG0480018380.01.T01 AT1G66040 34.507 142 75 4 114 244 262 396 3.54e-14 75.5
MsG0480018380.01.T01 AT5G39550 31.737 167 95 5 105 259 253 412 3.55e-14 75.5
MsG0480018380.01.T01 AT1G04050 28.230 209 93 7 280 433 407 613 2.53e-13 72.8
MsG0480018380.01.T01 AT1G04050 28.230 209 93 7 280 433 407 613 2.53e-13 72.8
MsG0480018380.01.T01 AT1G04050 47.222 72 37 1 363 433 438 509 3.34e-13 72.4

Find 108 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCTCGAACTATGTGATTT+TGG 0.106914 4:-4047678 None:intergenic
TCCTGAAGGGATGGAATTTA+AGG 0.160993 4:-4048813 None:intergenic
AGAATAGAGCTTAATATAAT+TGG 0.247368 4:+4047975 MsG0480018380.01.T01:CDS
TGGCCTTCATCGCCAGATTC+AGG 0.250113 4:+4047995 MsG0480018380.01.T01:CDS
ATATCCCCATTATGATTAAA+TGG 0.280457 4:-4048650 None:intergenic
GTTGAAGGGCACTTGCATTT+AGG 0.290171 4:-4048707 None:intergenic
TGCAACTAGTATTGTTTCTT+CGG 0.298661 4:+4048055 MsG0480018380.01.T01:CDS
CTTGAACTTGAGTCAGAATC+TGG 0.299589 4:-4048974 None:intergenic
GAGATTCCATTTAATCATAA+TGG 0.305009 4:+4048644 MsG0480018380.01.T01:CDS
AGATTCCATTTAATCATAAT+GGG 0.307894 4:+4048645 MsG0480018380.01.T01:CDS
CAATTTCTTCACTTCCTTCA+AGG 0.309539 4:-4048390 None:intergenic
GCAACTAGTATTGTTTCTTC+GGG 0.325203 4:+4048056 MsG0480018380.01.T01:CDS
AAAGGATGTAATTGTACTAA+TGG 0.326952 4:+4048575 MsG0480018380.01.T01:CDS
TTAGTACAATTACATCCTTT+TGG 0.326965 4:-4048572 None:intergenic
GTACGCAACAACATTTCTTA+TGG 0.328564 4:-4048607 None:intergenic
CAAGTTGGCTAACTCTATTA+TGG 0.333516 4:-4048733 None:intergenic
GAGTCATGTTGGCAGGATAT+AGG 0.343797 4:+4048305 MsG0480018380.01.T01:CDS
GGGATAAGATTGCAACATTC+AGG 0.352004 4:-4048548 None:intergenic
AATATCTTGGGATCTGTTCC+AGG 0.356395 4:+4047924 MsG0480018380.01.T01:CDS
TCCTTAAATTCCATCCCTTC+AGG 0.359885 4:+4048812 MsG0480018380.01.T01:CDS
AATCTATACCACCCTGAATC+TGG 0.363176 4:-4048007 None:intergenic
TACACCCCATCCCATTGAAT+TGG 0.382244 4:-4048786 None:intergenic
ATGCCGCTCAGTTTGGTAAT+GGG 0.406727 4:+4049029 MsG0480018380.01.T01:CDS
TCAACCAGAGCTTGCCTTGA+AGG 0.411478 4:+4048376 MsG0480018380.01.T01:CDS
ACTTCTTTCATATTTGACTT+TGG 0.416957 4:-4047834 None:intergenic
ACTTCCTTCAAGGCAAGCTC+TGG 0.417747 4:-4048380 None:intergenic
TCGGTTATGTATTTAAATGT+AGG 0.420707 4:-4048515 None:intergenic
AGTCATGTTGGCAGGATATA+GGG 0.423970 4:+4048306 MsG0480018380.01.T01:CDS
CGGTTATGTATTTAAATGTA+GGG 0.424018 4:-4048514 None:intergenic
CACCCTGAATCTGGCGATGA+AGG 0.425090 4:-4047998 None:intergenic
ACTATCGATGCCGCTCAGTT+TGG 0.425253 4:+4049022 MsG0480018380.01.T01:CDS
TTCTATGAAAAGCATGGAAC+AGG 0.428026 4:-4047785 None:intergenic
CTTGAGTCAGAATCTGGAAA+AGG 0.433257 4:-4048968 None:intergenic
TGGCGAGGTTCTTGAAGAAA+AGG 0.434427 4:+4048856 MsG0480018380.01.T01:CDS
TAATGAAGGGGAGAATATCT+TGG 0.437477 4:+4047911 MsG0480018380.01.T01:CDS
TAAAACCAATTCAATGGGAT+GGG 0.437648 4:+4048781 MsG0480018380.01.T01:CDS
CATAATAGAGTTAGCCAACT+TGG 0.449808 4:+4048734 MsG0480018380.01.T01:CDS
GAAAAGATACTCATCATTAC+CGG 0.451653 4:-4048894 None:intergenic
CTCTATTATGGCAGGTTGAA+GGG 0.459281 4:-4048721 None:intergenic
CAGAAGATCAAAAGCTTGAA+AGG 0.461492 4:+4048162 MsG0480018380.01.T01:CDS
GTTTAGCTTGCGAAGAATCC+CGG 0.462680 4:+4048352 MsG0480018380.01.T01:CDS
ACTCTATTATGGCAGGTTGA+AGG 0.468122 4:-4048722 None:intergenic
GGCCTTCATCGCCAGATTCA+GGG 0.471401 4:+4047996 MsG0480018380.01.T01:CDS
TTAAAACCAATTCAATGGGA+TGG 0.477591 4:+4048780 MsG0480018380.01.T01:CDS
CAAATATGAAAGAAGTAAAG+AGG 0.479183 4:+4047841 MsG0480018380.01.T01:CDS
AGAAAGATATATGTTTATGA+TGG 0.479440 4:+4048269 MsG0480018380.01.T01:CDS
TGTAGTTGAGTCATGTTGGC+AGG 0.480080 4:+4048298 MsG0480018380.01.T01:CDS
GAATTCTGTTAGAGTGATAC+GGG 0.482704 4:+4048220 MsG0480018380.01.T01:CDS
GATGCCGCTCAGTTTGGTAA+TGG 0.485332 4:+4049028 MsG0480018380.01.T01:CDS
GTTGAATATTTATTCCAAGT+TGG 0.486063 4:-4048748 None:intergenic
TATAGGGCCACGTGGGAAGC+TGG 0.486372 4:+4048322 MsG0480018380.01.T01:CDS
TTGAGTCAGAATCTGGAAAA+GGG 0.492327 4:-4048967 None:intergenic
AGAATTCTGTTAGAGTGATA+CGG 0.492353 4:+4048219 MsG0480018380.01.T01:CDS
AGAAGATCAAAAGCTTGAAA+GGG 0.499298 4:+4048163 MsG0480018380.01.T01:CDS
AATGAAGGGGAGAATATCTT+GGG 0.502787 4:+4047912 MsG0480018380.01.T01:CDS
TCTGTTCCAGGGGTTGAAGT+TGG 0.503371 4:+4047936 MsG0480018380.01.T01:CDS
AAGGGAGCTGTGTTAATGAA+GGG 0.505095 4:+4047898 MsG0480018380.01.T01:CDS
TGCAATCTTATCCCTCCAAA+AGG 0.515972 4:+4048557 MsG0480018380.01.T01:CDS
AAGGCAAGCTCTGGTTGACC+CGG 0.519854 4:-4048371 None:intergenic
AACAACAATAATCTTTGTGA+TGG 0.520060 4:+4048935 MsG0480018380.01.T01:CDS
TTGGCTAACTCTATTATGGC+AGG 0.524710 4:-4048729 None:intergenic
ACGGGGCGGCTATGAATCAA+TGG 0.525858 4:+4048238 MsG0480018380.01.T01:CDS
AAAACCAATTCAATGGGATG+GGG 0.526952 4:+4048782 MsG0480018380.01.T01:CDS
CTCAAGTTCAAGTGAAGTAG+TGG 0.532681 4:+4048985 MsG0480018380.01.T01:CDS
TTTAGCTTGCGAAGAATCCC+GGG 0.534568 4:+4048353 MsG0480018380.01.T01:CDS
GAAGGGAGCTGTGTTAATGA+AGG 0.538115 4:+4047897 MsG0480018380.01.T01:CDS
GATAAAACTTCCTGAAGGGA+TGG 0.541390 4:-4048822 None:intergenic
ATGAGAATGACTCAGTGGCC+AGG 0.542434 4:+4047739 MsG0480018380.01.T01:CDS
AAATCTTTCTATGAAAAGCA+TGG 0.545950 4:-4047791 None:intergenic
TTTATGTAGTTGAGTCATGT+TGG 0.552455 4:+4048294 MsG0480018380.01.T01:CDS
TCATCACCAACTTCAACCCC+TGG 0.553210 4:-4047942 None:intergenic
CAACTAGTATTGTTTCTTCG+GGG 0.557635 4:+4048057 MsG0480018380.01.T01:CDS
AGGGAGCTGTGTTAATGAAG+GGG 0.559797 4:+4047899 MsG0480018380.01.T01:CDS
TGTGTCGAAGATATTTCCTA+CGG 0.561049 4:+4048440 MsG0480018380.01.T01:CDS
AACTAGTATTGTTTCTTCGG+GGG 0.564319 4:+4048058 MsG0480018380.01.T01:CDS
CACAGATAAAACTTCCTGAA+GGG 0.565553 4:-4048826 None:intergenic
ATGGTGTTCACAGCACATAT+CGG 0.565881 4:-4048476 None:intergenic
GGTTATGTATTTAAATGTAG+GGG 0.572990 4:-4048513 None:intergenic
AAGAAACAATACTAGTTGCA+AGG 0.576224 4:-4048052 None:intergenic
TCACAGATAAAACTTCCTGA+AGG 0.579555 4:-4048827 None:intergenic
ATATCTTGGGATCTGTTCCA+GGG 0.585881 4:+4047925 MsG0480018380.01.T01:CDS
AGGCAAGCTCTGGTTGACCC+GGG 0.589790 4:-4048370 None:intergenic
ACTAGTATTGTTTCTTCGGG+GGG 0.593022 4:+4048059 MsG0480018380.01.T01:CDS
GTTATGTATTTAAATGTAGG+GGG 0.600728 4:-4048512 None:intergenic
TGCCGCTCAGTTTGGTAATG+GGG 0.603177 4:+4049030 MsG0480018380.01.T01:CDS
GTGTCGAAGATATTTCCTAC+GGG 0.605162 4:+4048441 MsG0480018380.01.T01:CDS
GGCGGCTATGAATCAATGGA+TGG 0.617291 4:+4048242 MsG0480018380.01.T01:CDS
AAGTTCAAGTGAAGTAGTGG+AGG 0.627990 4:+4048988 MsG0480018380.01.T01:CDS
GGACCACACTCATAGACTAG+AGG 0.629150 4:-4048686 None:intergenic
GATTCCATTTAATCATAATG+GGG 0.631488 4:+4048646 MsG0480018380.01.T01:CDS
CTTGGTCGTAGTAATTTGAG+TGG 0.634936 4:+4047714 MsG0480018380.01.T01:CDS
GAAGATCAAAAGCTTGAAAG+GGG 0.636952 4:+4048164 MsG0480018380.01.T01:CDS
ACATTCAGGATATATCATCT+CGG 0.654068 4:-4048534 None:intergenic
CTCAAATTACTACGACCAAG+AGG 0.664835 4:-4047711 None:intergenic
TGCTTTGAAGAACAGTAGTG+AGG 0.666121 4:+4048193 MsG0480018380.01.T01:CDS
TATCTTGGGATCTGTTCCAG+GGG 0.671761 4:+4047926 MsG0480018380.01.T01:CDS
CAGTGGCCAGGAATAAAGTG+AGG 0.677717 4:+4047751 MsG0480018380.01.T01:CDS
TATCTGTGAATATATTGGCG+AGG 0.678899 4:+4048841 MsG0480018380.01.T01:CDS
GGCAGGATATAGGGCCACGT+GGG 0.686351 4:+4048315 MsG0480018380.01.T01:CDS
AAGCCTCTAGTCTATGAGTG+TGG 0.686595 4:+4048683 MsG0480018380.01.T01:CDS
TTGATATATACAGGGCAGGG+TGG 0.688777 4:+4048113 MsG0480018380.01.T01:CDS
TGGCAGGATATAGGGCCACG+TGG 0.698447 4:+4048314 MsG0480018380.01.T01:CDS
CTCCCCATTACCAAACTGAG+CGG 0.701902 4:-4049032 None:intergenic
TCTGTTAGAGTGATACGGGG+CGG 0.725950 4:+4048224 MsG0480018380.01.T01:CDS
CTTCATCGCCAGATTCAGGG+TGG 0.729437 4:+4047999 MsG0480018380.01.T01:CDS
TTATAAACCAGCTTCCCACG+TGG 0.745113 4:-4048329 None:intergenic
TGGTCATGAGAATGACTCAG+TGG 0.759725 4:+4047734 MsG0480018380.01.T01:CDS
AATTCTGTTAGAGTGATACG+GGG 0.776374 4:+4048221 MsG0480018380.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTAAAACCAATTCAA+TGG + Chr4:4048775-4048794 MsG0480018380.01.T01:CDS 15.0%
!!! ATTTTTAAAACCAATTCAAT+GGG + Chr4:4048776-4048795 MsG0480018380.01.T01:CDS 15.0%
!! AGAAAGATATATGTTTATGA+TGG + Chr4:4048269-4048288 MsG0480018380.01.T01:CDS 20.0%
!! AGAATAGAGCTTAATATAAT+TGG + Chr4:4047975-4047994 MsG0480018380.01.T01:CDS 20.0%
!! AGATTCCATTTAATCATAAT+GGG + Chr4:4048645-4048664 MsG0480018380.01.T01:CDS 20.0%
!!! AAGTGTTTGTTATTTTCATT+AGG - Chr4:4047648-4047667 None:intergenic 20.0%
!!! AGTGTTTGTTATTTTCATTA+GGG - Chr4:4047647-4047666 None:intergenic 20.0%
!!! AGTTTTATCTGTGAATATAT+TGG + Chr4:4048836-4048855 MsG0480018380.01.T01:CDS 20.0%
! AAAAAGTTCAAAACAAGAGA+AGG + Chr4:4048413-4048432 MsG0480018380.01.T01:CDS 25.0%
! AAAGGATGTAATTGTACTAA+TGG + Chr4:4048575-4048594 MsG0480018380.01.T01:CDS 25.0%
! AAATCTTTCTATGAAAAGCA+TGG - Chr4:4047794-4047813 None:intergenic 25.0%
! AACAACAATAATCTTTGTGA+TGG + Chr4:4048935-4048954 MsG0480018380.01.T01:CDS 25.0%
! ATATCCCCATTATGATTAAA+TGG - Chr4:4048653-4048672 None:intergenic 25.0%
! CAAATATGAAAGAAGTAAAG+AGG + Chr4:4047841-4047860 MsG0480018380.01.T01:CDS 25.0%
! CGGTTATGTATTTAAATGTA+GGG - Chr4:4048517-4048536 None:intergenic 25.0%
! GAGATTCCATTTAATCATAA+TGG + Chr4:4048644-4048663 MsG0480018380.01.T01:CDS 25.0%
! GATTCCATTTAATCATAATG+GGG + Chr4:4048646-4048665 MsG0480018380.01.T01:CDS 25.0%
! GGTTATGTATTTAAATGTAG+GGG - Chr4:4048516-4048535 None:intergenic 25.0%
! GTTATGTATTTAAATGTAGG+GGG - Chr4:4048515-4048534 None:intergenic 25.0%
! GTTGAATATTTATTCCAAGT+TGG - Chr4:4048751-4048770 None:intergenic 25.0%
! TCGGTTATGTATTTAAATGT+AGG - Chr4:4048518-4048537 None:intergenic 25.0%
!! ACTTCTTTCATATTTGACTT+TGG - Chr4:4047837-4047856 None:intergenic 25.0%
!! TTAGTACAATTACATCCTTT+TGG - Chr4:4048575-4048594 None:intergenic 25.0%
!! TTTTCATAGAAAGATTTTGC+AGG + Chr4:4047797-4047816 MsG0480018380.01.T01:CDS 25.0%
!!! CAGATGTTTTGATATATACA+GGG + Chr4:4048105-4048124 MsG0480018380.01.T01:CDS 25.0%
!!! TCAGATGTTTTGATATATAC+AGG + Chr4:4048104-4048123 MsG0480018380.01.T01:CDS 25.0%
AAGAAACAATACTAGTTGCA+AGG - Chr4:4048055-4048074 None:intergenic 30.0%
ACATTCAGGATATATCATCT+CGG - Chr4:4048537-4048556 None:intergenic 30.0%
AGAAGATCAAAAGCTTGAAA+GGG + Chr4:4048163-4048182 MsG0480018380.01.T01:CDS 30.0%
AGAATTCTGTTAGAGTGATA+CGG + Chr4:4048219-4048238 MsG0480018380.01.T01:CDS 30.0%
GAAAAGATACTCATCATTAC+CGG - Chr4:4048897-4048916 None:intergenic 30.0%
TAAAACCAATTCAATGGGAT+GGG + Chr4:4048781-4048800 MsG0480018380.01.T01:CDS 30.0%
TTAAAACCAATTCAATGGGA+TGG + Chr4:4048780-4048799 MsG0480018380.01.T01:CDS 30.0%
TTTATGTAGTTGAGTCATGT+TGG + Chr4:4048294-4048313 MsG0480018380.01.T01:CDS 30.0%
! GATGAGTATCTTTTCGATAT+CGG + Chr4:4048902-4048921 MsG0480018380.01.T01:CDS 30.0%
! TACAATTACATCCTTTTGGA+GGG - Chr4:4048571-4048590 None:intergenic 30.0%
! TGCAACTAGTATTGTTTCTT+CGG + Chr4:4048055-4048074 MsG0480018380.01.T01:CDS 30.0%
!!! ATTTGACTTTGGTTTTGCTT+CGG - Chr4:4047826-4047845 None:intergenic 30.0%
AAAACCAATTCAATGGGATG+GGG + Chr4:4048782-4048801 MsG0480018380.01.T01:CDS 35.0%
AAGGAAGCTGAAAAAAGAAC+CGG + Chr4:4048875-4048894 MsG0480018380.01.T01:CDS 35.0%
AATGAAGGGGAGAATATCTT+GGG + Chr4:4047912-4047931 MsG0480018380.01.T01:CDS 35.0%
AATTCTGTTAGAGTGATACG+GGG + Chr4:4048221-4048240 MsG0480018380.01.T01:CDS 35.0%
CAATTTCTTCACTTCCTTCA+AGG - Chr4:4048393-4048412 None:intergenic 35.0%
CACAGATAAAACTTCCTGAA+GGG - Chr4:4048829-4048848 None:intergenic 35.0%
CAGAAGATCAAAAGCTTGAA+AGG + Chr4:4048162-4048181 MsG0480018380.01.T01:CDS 35.0%
CATAATAGAGTTAGCCAACT+TGG + Chr4:4048734-4048753 MsG0480018380.01.T01:CDS 35.0%
GAAGATCAAAAGCTTGAAAG+GGG + Chr4:4048164-4048183 MsG0480018380.01.T01:CDS 35.0%
GAATTCTGTTAGAGTGATAC+GGG + Chr4:4048220-4048239 MsG0480018380.01.T01:CDS 35.0%
GTACGCAACAACATTTCTTA+TGG - Chr4:4048610-4048629 None:intergenic 35.0%
TAATGAAGGGGAGAATATCT+TGG + Chr4:4047911-4047930 MsG0480018380.01.T01:CDS 35.0%
TATCTGTGAATATATTGGCG+AGG + Chr4:4048841-4048860 MsG0480018380.01.T01:CDS 35.0%
TCACAGATAAAACTTCCTGA+AGG - Chr4:4048830-4048849 None:intergenic 35.0%
TGTGTCGAAGATATTTCCTA+CGG + Chr4:4048440-4048459 MsG0480018380.01.T01:CDS 35.0%
TGTTGTTGCGTACTCAAAAA+TGG + Chr4:4048617-4048636 MsG0480018380.01.T01:CDS 35.0%
TTGAGTCAGAATCTGGAAAA+GGG - Chr4:4048970-4048989 None:intergenic 35.0%
! AACTAGTATTGTTTCTTCGG+GGG + Chr4:4048058-4048077 MsG0480018380.01.T01:CDS 35.0%
! AGCGTAAATGTTTTTCCTCT+TGG + Chr4:4047696-4047715 MsG0480018380.01.T01:CDS 35.0%
! CAACTAGTATTGTTTCTTCG+GGG + Chr4:4048057-4048076 MsG0480018380.01.T01:CDS 35.0%
! CAAGTTGGCTAACTCTATTA+TGG - Chr4:4048736-4048755 None:intergenic 35.0%
! CTTTCAAGCTTTTGATCTTC+TGG - Chr4:4048164-4048183 None:intergenic 35.0%
! GCAACTAGTATTGTTTCTTC+GGG + Chr4:4048056-4048075 MsG0480018380.01.T01:CDS 35.0%
! GTACAATTACATCCTTTTGG+AGG - Chr4:4048572-4048591 None:intergenic 35.0%
! TGTTTTCCTCACTTTATTCC+TGG - Chr4:4047760-4047779 None:intergenic 35.0%
! TTCTATGAAAAGCATGGAAC+AGG - Chr4:4047788-4047807 None:intergenic 35.0%
!! GTTTTGATATATACAGGGCA+GGG + Chr4:4048110-4048129 MsG0480018380.01.T01:CDS 35.0%
!!! TGTTTTGATATATACAGGGC+AGG + Chr4:4048109-4048128 MsG0480018380.01.T01:CDS 35.0%
AAGGGAGCTGTGTTAATGAA+GGG + Chr4:4047898-4047917 MsG0480018380.01.T01:CDS 40.0%
AAGTTCAAGTGAAGTAGTGG+AGG + Chr4:4048988-4049007 MsG0480018380.01.T01:CDS 40.0%
AATATCTTGGGATCTGTTCC+AGG + Chr4:4047924-4047943 MsG0480018380.01.T01:CDS 40.0%
AATCTATACCACCCTGAATC+TGG - Chr4:4048010-4048029 None:intergenic 40.0%
ACAAGCTGCGAAAAAGCTTA+AGG + Chr4:4047872-4047891 MsG0480018380.01.T01:CDS 40.0%
AGTCATGTTGGCAGGATATA+GGG + Chr4:4048306-4048325 MsG0480018380.01.T01:CDS 40.0%
ATATCTTGGGATCTGTTCCA+GGG + Chr4:4047925-4047944 MsG0480018380.01.T01:CDS 40.0%
ATGGTGTTCACAGCACATAT+CGG - Chr4:4048479-4048498 None:intergenic 40.0%
CGAAAAAGCTTAAGGAGGAA+GGG + Chr4:4047880-4047899 MsG0480018380.01.T01:CDS 40.0%
CTCAAATTACTACGACCAAG+AGG - Chr4:4047714-4047733 None:intergenic 40.0%
CTCAAGTTCAAGTGAAGTAG+TGG + Chr4:4048985-4049004 MsG0480018380.01.T01:CDS 40.0%
CTTGAACTTGAGTCAGAATC+TGG - Chr4:4048977-4048996 None:intergenic 40.0%
CTTGAGTCAGAATCTGGAAA+AGG - Chr4:4048971-4048990 None:intergenic 40.0%
CTTGGTCGTAGTAATTTGAG+TGG + Chr4:4047714-4047733 MsG0480018380.01.T01:CDS 40.0%
GATAAAACTTCCTGAAGGGA+TGG - Chr4:4048825-4048844 None:intergenic 40.0%
GGGATAAGATTGCAACATTC+AGG - Chr4:4048551-4048570 None:intergenic 40.0%
GTGTCGAAGATATTTCCTAC+GGG + Chr4:4048441-4048460 MsG0480018380.01.T01:CDS 40.0%
GTTGCGTACTCAAAAATGGA+GGG + Chr4:4048621-4048640 MsG0480018380.01.T01:CDS 40.0%
TCCTGAAGGGATGGAATTTA+AGG - Chr4:4048816-4048835 None:intergenic 40.0%
TCCTTAAATTCCATCCCTTC+AGG + Chr4:4048812-4048831 MsG0480018380.01.T01:CDS 40.0%
TGCAATCTTATCCCTCCAAA+AGG + Chr4:4048557-4048576 MsG0480018380.01.T01:CDS 40.0%
TGTTGCGTACTCAAAAATGG+AGG + Chr4:4048620-4048639 MsG0480018380.01.T01:CDS 40.0%
TTGCGTACTCAAAAATGGAG+GGG + Chr4:4048622-4048641 MsG0480018380.01.T01:CDS 40.0%
TTGGCTAACTCTATTATGGC+AGG - Chr4:4048732-4048751 None:intergenic 40.0%
! ACGCTCGAACTATGTGATTT+TGG - Chr4:4047681-4047700 None:intergenic 40.0%
! ACTAGTATTGTTTCTTCGGG+GGG + Chr4:4048059-4048078 MsG0480018380.01.T01:CDS 40.0%
! ACTCTATTATGGCAGGTTGA+AGG - Chr4:4048725-4048744 None:intergenic 40.0%
! CGCTCGAACTATGTGATTTT+GGG - Chr4:4047680-4047699 None:intergenic 40.0%
! CTCTATTATGGCAGGTTGAA+GGG - Chr4:4048724-4048743 None:intergenic 40.0%
!! TGCTTTGAAGAACAGTAGTG+AGG + Chr4:4048193-4048212 MsG0480018380.01.T01:CDS 40.0%
AAGCCTCTAGTCTATGAGTG+TGG + Chr4:4048683-4048702 MsG0480018380.01.T01:CDS 45.0%
AGCTGCGAAAAAGCTTAAGG+AGG + Chr4:4047875-4047894 MsG0480018380.01.T01:CDS 45.0%
AGGGAGCTGTGTTAATGAAG+GGG + Chr4:4047899-4047918 MsG0480018380.01.T01:CDS 45.0%
AGGGGGTTTCTCGTTATCTA+TGG - Chr4:4048498-4048517 None:intergenic 45.0%
GAAGGGAGCTGTGTTAATGA+AGG + Chr4:4047897-4047916 MsG0480018380.01.T01:CDS 45.0%
GAGTCATGTTGGCAGGATAT+AGG + Chr4:4048305-4048324 MsG0480018380.01.T01:CDS 45.0%
GCGAAAAAGCTTAAGGAGGA+AGG + Chr4:4047879-4047898 MsG0480018380.01.T01:CDS 45.0%
TACACCCCATCCCATTGAAT+TGG - Chr4:4048789-4048808 None:intergenic 45.0%
TATCTTGGGATCTGTTCCAG+GGG + Chr4:4047926-4047945 MsG0480018380.01.T01:CDS 45.0%
TGGCGAGGTTCTTGAAGAAA+AGG + Chr4:4048856-4048875 MsG0480018380.01.T01:CDS 45.0%
TGGTCATGAGAATGACTCAG+TGG + Chr4:4047734-4047753 MsG0480018380.01.T01:CDS 45.0%
TGTAGTTGAGTCATGTTGGC+AGG + Chr4:4048298-4048317 MsG0480018380.01.T01:CDS 45.0%
TTATAAACCAGCTTCCCACG+TGG - Chr4:4048332-4048351 None:intergenic 45.0%
TTGATATATACAGGGCAGGG+TGG + Chr4:4048113-4048132 MsG0480018380.01.T01:CDS 45.0%
! ATGCCGCTCAGTTTGGTAAT+GGG + Chr4:4049029-4049048 MsG0480018380.01.T01:CDS 45.0%
! CGGAATTTTCTCTTTCCCGT+AGG - Chr4:4048459-4048478 None:intergenic 45.0%
!! GTTGAAGGGCACTTGCATTT+AGG - Chr4:4048710-4048729 None:intergenic 45.0%
!! GTTTAGCTTGCGAAGAATCC+CGG + Chr4:4048352-4048371 MsG0480018380.01.T01:CDS 45.0%
!! TTTAGCTTGCGAAGAATCCC+GGG + Chr4:4048353-4048372 MsG0480018380.01.T01:CDS 45.0%
ACTATCGATGCCGCTCAGTT+TGG + Chr4:4049022-4049041 MsG0480018380.01.T01:CDS 50.0%
ACTTCCTTCAAGGCAAGCTC+TGG - Chr4:4048383-4048402 None:intergenic 50.0%
ATGAGAATGACTCAGTGGCC+AGG + Chr4:4047739-4047758 MsG0480018380.01.T01:CDS 50.0%
CAGTGGCCAGGAATAAAGTG+AGG + Chr4:4047751-4047770 MsG0480018380.01.T01:CDS 50.0%
CTCCCCATTACCAAACTGAG+CGG - Chr4:4049035-4049054 None:intergenic 50.0%
GATGCCGCTCAGTTTGGTAA+TGG + Chr4:4049028-4049047 MsG0480018380.01.T01:CDS 50.0%
GGACCACACTCATAGACTAG+AGG - Chr4:4048689-4048708 None:intergenic 50.0%
GGCGGCTATGAATCAATGGA+TGG + Chr4:4048242-4048261 MsG0480018380.01.T01:CDS 50.0%
TCAACCAGAGCTTGCCTTGA+AGG + Chr4:4048376-4048395 MsG0480018380.01.T01:CDS 50.0%
TCATCACCAACTTCAACCCC+TGG - Chr4:4047945-4047964 None:intergenic 50.0%
TCTGTTAGAGTGATACGGGG+CGG + Chr4:4048224-4048243 MsG0480018380.01.T01:CDS 50.0%
TCTGTTCCAGGGGTTGAAGT+TGG + Chr4:4047936-4047955 MsG0480018380.01.T01:CDS 50.0%
!! TGCCGCTCAGTTTGGTAATG+GGG + Chr4:4049030-4049049 MsG0480018380.01.T01:CDS 50.0%
ACGGGGCGGCTATGAATCAA+TGG + Chr4:4048238-4048257 MsG0480018380.01.T01:CDS 55.0%
CACCCTGAATCTGGCGATGA+AGG - Chr4:4048001-4048020 None:intergenic 55.0%
CTTCATCGCCAGATTCAGGG+TGG + Chr4:4047999-4048018 MsG0480018380.01.T01:CDS 55.0%
GGCCTTCATCGCCAGATTCA+GGG + Chr4:4047996-4048015 MsG0480018380.01.T01:CDS 55.0%
TGGCCTTCATCGCCAGATTC+AGG + Chr4:4047995-4048014 MsG0480018380.01.T01:CDS 55.0%
! AAGGCAAGCTCTGGTTGACC+CGG - Chr4:4048374-4048393 None:intergenic 55.0%
GGCAGGATATAGGGCCACGT+GGG + Chr4:4048315-4048334 MsG0480018380.01.T01:CDS 60.0%
TATAGGGCCACGTGGGAAGC+TGG + Chr4:4048322-4048341 MsG0480018380.01.T01:CDS 60.0%
TGGCAGGATATAGGGCCACG+TGG + Chr4:4048314-4048333 MsG0480018380.01.T01:CDS 60.0%
! AGGCAAGCTCTGGTTGACCC+GGG - Chr4:4048373-4048392 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 4047606 4049063 4047606 ID=MsG0480018380.01;Name=MsG0480018380.01
Chr4 mRNA 4047606 4049063 4047606 ID=MsG0480018380.01.T01;Parent=MsG0480018380.01;Name=MsG0480018380.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|485
Chr4 exon 4047606 4049063 4047606 ID=MsG0480018380.01.T01:exon:1129;Parent=MsG0480018380.01.T01
Chr4 CDS 4047606 4049063 4047606 ID=MsG0480018380.01.T01:cds;Parent=MsG0480018380.01.T01
Gene Sequence

>MsG0480018380.01.T01

ATGGGTCAATTAGTAACGTTAGAGAAGAGTTCTCAAGACCCTAATGAAAATAACAAACACTTTAAAAGTGTCCCAAAATCACATAGTTCGAGCGTAAATGTTTTTCCTCTTGGTCGTAGTAATTTGAGTGGTCATGAGAATGACTCAGTGGCCAGGAATAAAGTGAGGAAAACATTGCGCCTGTTCCATGCTTTTCATAGAAAGATTTTGCAGGAAGCCGAAGCAAAACCAAAGTCAAATATGAAAGAAGTAAAGAGGTTTGATTTACAAGCTGCGAAAAAGCTTAAGGAGGAAGGGAGCTGTGTTAATGAAGGGGAGAATATCTTGGGATCTGTTCCAGGGGTTGAAGTTGGTGATGAATTTCAGTTCAGAATAGAGCTTAATATAATTGGCCTTCATCGCCAGATTCAGGGTGGTATAGATTATGTGAAACAAAAAGACAAGATCCTTGCAACTAGTATTGTTTCTTCGGGGGGCTATGCTGATGACTTAGATAACTCAGATGTTTTGATATATACAGGGCAGGGTGGAAATGTGATGAGCAGTGATAAAGAGCCAGAAGATCAAAAGCTTGAAAGGGGAAATCTTGCTTTGAAGAACAGTAGTGAGGTAAAGAATTCTGTTAGAGTGATACGGGGCGGCTATGAATCAATGGATGGTAAAAGAAAGATATATGTTTATGATGGACTTTATGTAGTTGAGTCATGTTGGCAGGATATAGGGCCACGTGGGAAGCTGGTTTATAAGTTTAGCTTGCGAAGAATCCCGGGTCAACCAGAGCTTGCCTTGAAGGAAGTGAAGAAATTGAAAAAGTTCAAAACAAGAGAAGGTCTATGTGTCGAAGATATTTCCTACGGGAAAGAGAAAATTCCGATATGTGCTGTGAACACCATAGATAACGAGAAACCCCCTACATTTAAATACATAACCGAGATGATATATCCTGAATGTTGCAATCTTATCCCTCCAAAAGGATGTAATTGTACTAATGGATGCTCTGACCATAAGAAATGTTGTTGCGTACTCAAAAATGGAGGGGAGATTCCATTTAATCATAATGGGGATATTGTAGAAGTAAAGCCTCTAGTCTATGAGTGTGGTCCTAAATGCAAGTGCCCTTCAACCTGCCATAATAGAGTTAGCCAACTTGGAATAAATATTCAACTCGAAATTTTTAAAACCAATTCAATGGGATGGGGTGTAAGATCCTTAAATTCCATCCCTTCAGGAAGTTTTATCTGTGAATATATTGGCGAGGTTCTTGAAGAAAAGGAAGCTGAAAAAAGAACCGGTAATGATGAGTATCTTTTCGATATCGGAAATAACAAAAACAACAATAATCTTTGTGATGGACTTTCAAACCCTTTTCCAGATTCTGACTCAAGTTCAAGTGAAGTAGTGGAGGATTCTGACTTCACTATCGATGCCGCTCAGTTTGGTAATGGGGAGATTTGTTAA
Protein sequence

>MsG0480018380.01.T01

MGQLVTLEKSSQDPNENNKHFKSVPKSHSSSVNVFPLGRSNLSGHENDSVARNKVRKTLRLFHAFHRKILQEAEAKPKSNMKEVKRFDLQAAKKLKEEGSCVNEGENILGSVPGVEVGDEFQFRIELNIIGLHRQIQGGIDYVKQKDKILATSIVSSGGYADDLDNSDVLIYTGQGGNVMSSDKEPEDQKLERGNLALKNSSEVKNSVRVIRGGYESMDGKRKIYVYDGLYVVESCWQDIGPRGKLVYKFSLRRIPGQPELALKEVKKLKKFKTREGLCVEDISYGKEKIPICAVNTIDNEKPPTFKYITEMIYPECCNLIPPKGCNCTNGCSDHKKCCCVLKNGGEIPFNHNGDIVEVKPLVYECGPKCKCPSTCHNRVSQLGINIQLEIFKTNSMGWGVRSLNSIPSGSFICEYIGEVLEEKEAEKRTGNDEYLFDIGNNKNNNNLCDGLSNPFPDSDSSSSEVVEDSDFTIDAAQFGNGEIC*