AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018953.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018953.01.T01 MTR_1g016370 56.540 237 35 4 9 177 45 281 1.24e-80 246
MsG0480018953.01.T01 MTR_8g054100 65.730 178 32 4 9 157 45 222 7.39e-72 221
MsG0480018953.01.T01 MTR_5g055390 56.522 207 39 5 9 177 48 241 3.39e-65 205
MsG0480018953.01.T01 MTR_4g061370 51.282 234 34 5 9 177 1057 1275 7.60e-59 202
MsG0480018953.01.T01 MTR_2g018710 38.397 237 78 4 7 175 45 281 3.48e-42 147
MsG0480018953.01.T01 MTR_3g110155 36.842 247 81 4 6 177 43 289 4.08e-41 144
MsG0480018953.01.T01 MTR_8g006585 36.842 247 81 4 6 177 43 289 4.08e-41 144
MsG0480018953.01.T01 MTR_8g006695 36.842 247 81 4 6 177 43 289 4.08e-41 144
MsG0480018953.01.T01 MTR_1g080080 37.975 237 79 4 7 175 45 281 5.86e-41 144
MsG0480018953.01.T01 MTR_2g086350 37.975 237 79 4 7 175 45 281 5.99e-41 144
MsG0480018953.01.T01 MTR_2g435870 36.937 222 89 7 6 177 29 249 2.65e-33 123
MsG0480018953.01.T01 MTR_5g054570 33.333 237 72 5 7 175 45 263 5.18e-28 109
MsG0480018953.01.T01 MTR_2g054730 53.922 102 11 1 112 177 11 112 1.21e-26 102
MsG0480018953.01.T01 MTR_2g436180 38.065 155 56 4 63 177 1 155 3.29e-16 75.5
MsG0480018953.01.T01 MTR_4g043620 43.158 95 52 2 14 106 45 139 3.15e-14 71.6
MsG0480018953.01.T01 MTR_1g073440 41.176 85 49 1 14 97 45 129 9.53e-13 67.4
MsG0480018953.01.T01 MTR_2g021280 38.947 95 56 2 14 106 45 139 1.40e-11 64.3
MsG0480018953.01.T01 MTR_4g046743 37.500 104 29 1 108 175 129 232 1.89e-11 63.2
MsG0480018953.01.T01 MTR_5g018260 32.414 145 78 4 14 138 44 188 2.53e-11 63.2
MsG0480018953.01.T01 MTR_8g069895 38.947 95 56 2 14 106 45 139 2.96e-11 63.2
MsG0480018953.01.T01 MTR_7g105890 41.429 70 40 1 22 91 46 114 7.41e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 63 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCATGGTATAACTTATT+TGG 0.134716 4:+12301214 MsG0480018953.01.T01:CDS
ACAACTGAATTTGGAAATAT+TGG 0.148866 4:+12301612 MsG0480018953.01.T01:CDS
AACCCTCTTTATGAAAATTT+AGG 0.203461 4:+12301403 MsG0480018953.01.T01:CDS
GTTGGAGCAACAACTGAATT+TGG 0.213211 4:+12301603 MsG0480018953.01.T01:CDS
CTTGTAAGTTTCCTCAGTTC+TGG 0.261724 4:-12304344 None:intergenic
ATGAGCCTGATGAAGAATTA+TGG 0.261816 4:+12304247 MsG0480018953.01.T01:CDS
AACTTATTTGGGAGAGAAAC+TGG 0.287945 4:+12301226 MsG0480018953.01.T01:CDS
TTGGGAGAGAAACTGGATTA+GGG 0.298488 4:+12301233 MsG0480018953.01.T01:CDS
TGAAGAATTATGGGATATTC+TGG 0.315131 4:+12304257 MsG0480018953.01.T01:CDS
TTTGGGAGAGAAACTGGATT+AGG 0.315464 4:+12301232 MsG0480018953.01.T01:CDS
AAACTTTGTGAAAGCATGTT+TGG 0.334075 4:+12301049 None:intergenic
GTCCGGTTAAATTATGAAAT+CGG 0.352053 4:-12301135 None:intergenic
TGTATTACATACCAGTTGTT+TGG 0.352266 4:-12301305 None:intergenic
CAACTGAATTTGGAAATATT+GGG 0.356640 4:+12301613 MsG0480018953.01.T01:CDS
GAGCATGGTATAACTTATTT+GGG 0.363157 4:+12301215 MsG0480018953.01.T01:CDS
GTCCGATTTCATAATTTAAC+CGG 0.374307 4:+12301133 MsG0480018953.01.T01:CDS
AAACATGACTACTCCTGTTC+TGG 0.376898 4:+12304089 MsG0480018953.01.T01:CDS
TTGGTCTCCCACCACTCATC+AGG 0.410369 4:-12301286 None:intergenic
TCAGCATCTCCATTGAACTT+TGG 0.415028 4:-12304185 None:intergenic
ATGATCTTTATTTGCATGCT+CGG 0.426517 4:+12301854 MsG0480018953.01.T01:CDS
TGAGCCTGATGAAGAATTAT+GGG 0.433042 4:+12304248 MsG0480018953.01.T01:CDS
GGAAAAGTTCCAAAGTTCAA+TGG 0.457730 4:+12304176 MsG0480018953.01.T01:CDS
ATATCCCATAATTCTTCATC+AGG 0.458489 4:-12304252 None:intergenic
TGAGAGGCAGTGGCTGTAGA+AGG 0.458696 4:+12301555 MsG0480018953.01.T01:intron
AGTATCACTAGAACCAGAAC+AGG 0.461415 4:-12304102 None:intergenic
AGAAGGCTCAACTAAAGAAT+AGG 0.479515 4:+12301167 MsG0480018953.01.T01:CDS
CTCGTACCAAACGTTCTCCT+TGG 0.491631 4:-12301081 None:intergenic
AGCCTTCTCATACTTCAGTC+CGG 0.504703 4:-12301152 None:intergenic
GAAAAGTGTGTTTAATCACT+TGG 0.505105 4:-12301444 None:intergenic
GCTACAACAGTGTTGTTCAT+AGG 0.513387 4:-12301262 None:intergenic
AAGTCTCCAAGGAGAACGTT+TGG 0.518032 4:+12301075 MsG0480018953.01.T01:CDS
GCAGTGGCTGTAGAAGGATC+TGG 0.527670 4:+12301561 MsG0480018953.01.T01:intron
ATGACAGTCTGAGAAAGGAT+TGG 0.531322 4:+12301191 MsG0480018953.01.T01:CDS
GTGGTGGGAGACCAAACAAC+TGG 0.536532 4:+12301294 MsG0480018953.01.T01:CDS
TTTATGAAAATTTAGGAACA+AGG 0.537141 4:+12301410 MsG0480018953.01.T01:CDS
TTATGGGATATTCTGGAAGA+TGG 0.538253 4:+12304264 MsG0480018953.01.T01:CDS
GATAGTAAGAAAACCAGTGT+TGG 0.541975 4:+12304155 MsG0480018953.01.T01:CDS
TGTGTTTAATCACTTGGCGA+TGG 0.542355 4:-12301438 None:intergenic
GAGATGCTGAAGAGTTCTCT+TGG 0.543033 4:+12304198 MsG0480018953.01.T01:CDS
TTATGAAAATTTAGGAACAA+GGG 0.543148 4:+12301411 MsG0480018953.01.T01:CDS
TAGGTATGACAGTCTGAGAA+AGG 0.543958 4:+12301186 MsG0480018953.01.T01:CDS
GGAGAGAAACTGGATTAGGG+TGG 0.543958 4:+12301236 MsG0480018953.01.T01:CDS
GATTTAGTCCTTGATGAAGA+AGG 0.545046 4:+12304294 MsG0480018953.01.T01:CDS
ATCATTTGAGACTTCATCCA+TGG 0.558263 4:-12301836 None:intergenic
GTCAAAAGAGTGGTGAGAAG+AGG 0.562252 4:+12301661 MsG0480018953.01.T01:CDS
TATGAAAATTTAGGAACAAG+GGG 0.564824 4:+12301412 MsG0480018953.01.T01:CDS
TCATGATGTAACTGTAAAAG+TGG 0.568608 4:-12304228 None:intergenic
CAGCAGCTCCTTCTTCATCA+AGG 0.571109 4:-12304302 None:intergenic
TGATGAAGAAGGAGCTGCTG+TGG 0.575095 4:+12304305 MsG0480018953.01.T01:CDS
GATAGCGAGGATGCAAGTGT+TGG 0.599986 4:+12301585 MsG0480018953.01.T01:CDS
ATGCTGAAGAGTTCTCTTGG+TGG 0.602563 4:+12304201 MsG0480018953.01.T01:CDS
ATGTTTGGAGCAAGTCTCCA+AGG 0.602623 4:+12301064 MsG0480018953.01.T01:CDS
TGAGAAAGGATTGGAGAGCA+TGG 0.612846 4:+12301200 MsG0480018953.01.T01:CDS
GAGAGAAACTGGATTAGGGT+GGG 0.621771 4:+12301237 MsG0480018953.01.T01:CDS
AGAAGGATCTGGCGATAGCG+AGG 0.624952 4:+12301572 MsG0480018953.01.T01:CDS
AACCGGACTGAAGTATGAGA+AGG 0.626830 4:+12301150 MsG0480018953.01.T01:CDS
AAAACTGATGGTCAAAAGAG+TGG 0.632803 4:+12301651 MsG0480018953.01.T01:CDS
TTGTAGCACCTGATGAGTGG+TGG 0.632872 4:+12301278 MsG0480018953.01.T01:CDS
TGTAGCACCTGATGAGTGGT+GGG 0.651018 4:+12301279 MsG0480018953.01.T01:CDS
GATGGCTGAGCTTCACACCA+TGG 0.663371 4:+12301819 MsG0480018953.01.T01:CDS
GATATTCTGGAAGATGGTGT+TGG 0.673336 4:+12304270 MsG0480018953.01.T01:CDS
AAGAACATACTCCAGAACTG+AGG 0.695172 4:+12304333 MsG0480018953.01.T01:CDS
CTGTTGTAGCACCTGATGAG+TGG 0.705267 4:+12301275 MsG0480018953.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAATTAAGAAATAATCT+AGG + Chr4:12302122-12302141 MsG0480018953.01.T01:intron 10.0%
!! AGGTATTAAAAATTGTAAAT+TGG + Chr4:12302619-12302638 MsG0480018953.01.T01:intron 15.0%
!! ATGATGTAACTTTATAATTA+TGG - Chr4:12302675-12302694 None:intergenic 15.0%
!! ATTACACAGAAAATTGTTTA+AGG - Chr4:12303614-12303633 None:intergenic 20.0%
!! TTATAAAGTTACATCATCAT+TGG + Chr4:12302678-12302697 MsG0480018953.01.T01:intron 20.0%
!! TTATGAAAATTTAGGAACAA+GGG + Chr4:12301411-12301430 MsG0480018953.01.T01:CDS 20.0%
!! TTTATGAAAATTTAGGAACA+AGG + Chr4:12301410-12301429 MsG0480018953.01.T01:CDS 20.0%
!!! ATTTCAAATTTGTTCTTCAT+AGG + Chr4:12303275-12303294 MsG0480018953.01.T01:intron 20.0%
!!! GATAATTATGCTTGTATTTT+TGG + Chr4:12302290-12302309 MsG0480018953.01.T01:intron 20.0%
!!! TTATGTTTTTCCTTTCAATT+AGG + Chr4:12302170-12302189 MsG0480018953.01.T01:intron 20.0%
!!! TTCCTAAATTTTCATAAAGA+GGG - Chr4:12301408-12301427 None:intergenic 20.0%
!!! TTCTGAACTATATTTGTTTT+AGG + Chr4:12302396-12302415 MsG0480018953.01.T01:intron 20.0%
! AAAAAAAGGTGGACAATATT+AGG + Chr4:12301110-12301129 MsG0480018953.01.T01:CDS 25.0%
! AAAAAAGGTGGACAATATTA+GGG + Chr4:12301111-12301130 MsG0480018953.01.T01:CDS 25.0%
! AACCCTCTTTATGAAAATTT+AGG + Chr4:12301403-12301422 MsG0480018953.01.T01:CDS 25.0%
! AATTCTTCACAAAAAACTGA+TGG + Chr4:12301639-12301658 MsG0480018953.01.T01:CDS 25.0%
! ACAACATAAGTCTTCATTAA+GGG - Chr4:12302268-12302287 None:intergenic 25.0%
! ACAACTGAATTTGGAAATAT+TGG + Chr4:12301612-12301631 MsG0480018953.01.T01:CDS 25.0%
! AGTTACAAAGATAAGATTGT+TGG + Chr4:12302549-12302568 MsG0480018953.01.T01:intron 25.0%
! CAACTGAATTTGGAAATATT+GGG + Chr4:12301613-12301632 MsG0480018953.01.T01:CDS 25.0%
! GTTATCATCAAAACTCTTTA+AGG - Chr4:12302598-12302617 None:intergenic 25.0%
! TAATATTTGTTCAAGTGTAC+AGG + Chr4:12302648-12302667 MsG0480018953.01.T01:intron 25.0%
! TACAATACTTCCTAATTGAA+AGG - Chr4:12302183-12302202 None:intergenic 25.0%
! TATGAAAATTTAGGAACAAG+GGG + Chr4:12301412-12301431 MsG0480018953.01.T01:CDS 25.0%
!! AAACACAAAAATTTGAAGCT+TGG + Chr4:12302729-12302748 MsG0480018953.01.T01:intron 25.0%
!! GTTCCTAAATTTTCATAAAG+AGG - Chr4:12301409-12301428 None:intergenic 25.0%
!! TTTTGCAAATAACTATGATC+AGG - Chr4:12302330-12302349 None:intergenic 25.0%
!!! AAAGAGTTTTGATGATAACA+AGG + Chr4:12302599-12302618 MsG0480018953.01.T01:intron 25.0%
!!! CTGTGTAATTTTGTTTAGTT+AGG + Chr4:12303625-12303644 MsG0480018953.01.T01:intron 25.0%
AACACGAGTATACATATCTA+AGG - Chr4:12301351-12301370 None:intergenic 30.0%
AACACTTGATATACAATCAG+AGG - Chr4:12303588-12303607 None:intergenic 30.0%
AATGAGCAAATAAAGAGCTA+TGG + Chr4:12302706-12302725 MsG0480018953.01.T01:intron 30.0%
AGAGCATGGTATAACTTATT+TGG + Chr4:12301214-12301233 MsG0480018953.01.T01:CDS 30.0%
ATATCCCATAATTCTTCATC+AGG - Chr4:12304255-12304274 None:intergenic 30.0%
CACAACATAAGTCTTCATTA+AGG - Chr4:12302269-12302288 None:intergenic 30.0%
GAGCATGGTATAACTTATTT+GGG + Chr4:12301215-12301234 MsG0480018953.01.T01:CDS 30.0%
GTCCGATTTCATAATTTAAC+CGG + Chr4:12301133-12301152 MsG0480018953.01.T01:CDS 30.0%
GTCCGGTTAAATTATGAAAT+CGG - Chr4:12301138-12301157 None:intergenic 30.0%
TAATCAGAATCTAAATGCAG+CGG - Chr4:12303890-12303909 None:intergenic 30.0%
TCATGATGTAACTGTAAAAG+TGG - Chr4:12304231-12304250 None:intergenic 30.0%
TCTTTCACACCTCTATATAA+AGG + Chr4:12303384-12303403 MsG0480018953.01.T01:intron 30.0%
TGAAGAATTATGGGATATTC+TGG + Chr4:12304257-12304276 MsG0480018953.01.T01:CDS 30.0%
TGATGTGAGTTATAAAATGC+AGG + Chr4:12301378-12301397 MsG0480018953.01.T01:intron 30.0%
TGTATTACATACCAGTTGTT+TGG - Chr4:12301308-12301327 None:intergenic 30.0%
! ATGATAATGATGATGATGAG+AGG + Chr4:12301539-12301558 MsG0480018953.01.T01:intron 30.0%
! ATGATCTTTATTTGCATGCT+CGG + Chr4:12301854-12301873 MsG0480018953.01.T01:CDS 30.0%
! GAAAAGTGTGTTTAATCACT+TGG - Chr4:12301447-12301466 None:intergenic 30.0%
! GTGATTAAACACACTTTTCA+AGG + Chr4:12301448-12301467 MsG0480018953.01.T01:CDS 30.0%
! TCATCATTTTCAAACACAGA+TGG - Chr4:12301523-12301542 None:intergenic 30.0%
!! TTAGGAAGTATTGTAAGAAG+TGG + Chr4:12302188-12302207 MsG0480018953.01.T01:intron 30.0%
AAAACTGATGGTCAAAAGAG+TGG + Chr4:12301651-12301670 MsG0480018953.01.T01:CDS 35.0%
AACTTATTTGGGAGAGAAAC+TGG + Chr4:12301226-12301245 MsG0480018953.01.T01:CDS 35.0%
AAGAAAACAGCTCCAGAATA+CGG + Chr4:12303215-12303234 MsG0480018953.01.T01:intron 35.0%
AAGGTGAGAAAAACACAAGA+AGG - Chr4:12304002-12304021 None:intergenic 35.0%
AATTTACTAGCAAGTGCAGA+AGG - Chr4:12302495-12302514 None:intergenic 35.0%
ACGACACAAAGCAATTATAC+TGG - Chr4:12303842-12303861 None:intergenic 35.0%
AGAAGGCTCAACTAAAGAAT+AGG + Chr4:12301167-12301186 MsG0480018953.01.T01:CDS 35.0%
AGGTGAGAAAAACACAAGAA+GGG - Chr4:12304001-12304020 None:intergenic 35.0%
ATATTCAGCTTCTGGTGAAA+TGG - Chr4:12302977-12302996 None:intergenic 35.0%
ATGAGCCTGATGAAGAATTA+TGG + Chr4:12304247-12304266 MsG0480018953.01.T01:CDS 35.0%
ATGTTCCTTTGGATAAGGAA+TGG + Chr4:12303252-12303271 MsG0480018953.01.T01:intron 35.0%
CAAATCAGATACAGACTCAA+TGG - Chr4:12303554-12303573 None:intergenic 35.0%
CAAATCAGGCAAAAGATCAT+TGG + Chr4:12303306-12303325 MsG0480018953.01.T01:intron 35.0%
CATAGATGTTCCTTTGGATA+AGG + Chr4:12303247-12303266 MsG0480018953.01.T01:intron 35.0%
GAAACACAAACAAGGATTAG+TGG + Chr4:12303061-12303080 MsG0480018953.01.T01:intron 35.0%
GAAATCCATTCCTTATCCAA+AGG - Chr4:12303260-12303279 None:intergenic 35.0%
GATAGTAAGAAAACCAGTGT+TGG + Chr4:12304155-12304174 MsG0480018953.01.T01:CDS 35.0%
GATTTAGTCCTTGATGAAGA+AGG + Chr4:12304294-12304313 MsG0480018953.01.T01:CDS 35.0%
GGAAAAGTTCCAAAGTTCAA+TGG + Chr4:12304176-12304195 MsG0480018953.01.T01:CDS 35.0%
GTCTTCACTCCTTTATATAG+AGG - Chr4:12303396-12303415 None:intergenic 35.0%
TACTCATAGTGAAATCTCCT+TGG + Chr4:12303774-12303793 MsG0480018953.01.T01:intron 35.0%
TCAATGCAAAAGCTGTCTAT+TGG + Chr4:12303034-12303053 MsG0480018953.01.T01:intron 35.0%
TCTTGCATTACCAATTCTCT+AGG - Chr4:12303418-12303437 None:intergenic 35.0%
TGAGCCTGATGAAGAATTAT+GGG + Chr4:12304248-12304267 MsG0480018953.01.T01:CDS 35.0%
TTGATTGTGTTCTTGAGCAA+AGG + Chr4:12303682-12303701 MsG0480018953.01.T01:intron 35.0%
TTGGATTCGAAACACAAACA+AGG + Chr4:12303053-12303072 MsG0480018953.01.T01:intron 35.0%
! ACGAGCTTTTCAAAAAAAGG+TGG + Chr4:12301099-12301118 MsG0480018953.01.T01:CDS 35.0%
! ATCATTTGAGACTTCATCCA+TGG - Chr4:12301839-12301858 None:intergenic 35.0%
! CAATGATCTTTTGCCTGATT+TGG - Chr4:12303308-12303327 None:intergenic 35.0%
! GATCAGTGTGTTCAGTTTTT+AGG + Chr4:12303939-12303958 MsG0480018953.01.T01:intron 35.0%
! GGTACGAGCTTTTCAAAAAA+AGG + Chr4:12301096-12301115 MsG0480018953.01.T01:CDS 35.0%
! GTGTTTTTCTCACCTTCAAT+TGG + Chr4:12304006-12304025 MsG0480018953.01.T01:intron 35.0%
! TTATGGGATATTCTGGAAGA+TGG + Chr4:12304264-12304283 MsG0480018953.01.T01:CDS 35.0%
!! GTCATTTCAAAGGTGTTTGA+AGG + Chr4:12303095-12303114 MsG0480018953.01.T01:intron 35.0%
!!! TGCACTTGCTTTTTCTTACT+TGG - Chr4:12301711-12301730 None:intergenic 35.0%
AAACATGACTACTCCTGTTC+TGG + Chr4:12304089-12304108 MsG0480018953.01.T01:CDS 40.0%
AAGCTTCAACAACGACTCTT+TGG + Chr4:12303338-12303357 MsG0480018953.01.T01:intron 40.0%
AAGTGATACACAACAGACCA+AGG - Chr4:12304041-12304060 None:intergenic 40.0%
ACCAGAAGCTGAATATCAAC+TGG + Chr4:12302981-12303000 MsG0480018953.01.T01:intron 40.0%
AGTATCACTAGAACCAGAAC+AGG - Chr4:12304105-12304124 None:intergenic 40.0%
ATCCAAAGGAACATCTATGC+AGG - Chr4:12303246-12303265 None:intergenic 40.0%
ATGACAGTCTGAGAAAGGAT+TGG + Chr4:12301191-12301210 MsG0480018953.01.T01:CDS 40.0%
ATGCAGAACAATTTCAGCAG+AGG - Chr4:12303482-12303501 None:intergenic 40.0%
CATAGGACAACAACCAAATC+AGG + Chr4:12303292-12303311 MsG0480018953.01.T01:intron 40.0%
GAAATAAACATCTCCCTTGC+AGG - Chr4:12301903-12301922 None:intergenic 40.0%
GAACAATTTCAGCAGAGGTT+TGG - Chr4:12303477-12303496 None:intergenic 40.0%
GAGATGTTTATTTCCCTACG+AGG + Chr4:12301909-12301928 MsG0480018953.01.T01:intron 40.0%
GGATGTATGGGAATTAAGCT+AGG + Chr4:12302008-12302027 MsG0480018953.01.T01:intron 40.0%
GGTGAGAAAAACACAAGAAG+GGG - Chr4:12304000-12304019 None:intergenic 40.0%
GTGAGAAAAACACAAGAAGG+GGG - Chr4:12303999-12304018 None:intergenic 40.0%
GTTGGAGCAACAACTGAATT+TGG + Chr4:12301603-12301622 MsG0480018953.01.T01:CDS 40.0%
GTTTGCAACGGTCATTTCAA+AGG + Chr4:12303085-12303104 MsG0480018953.01.T01:intron 40.0%
TAGGTATGACAGTCTGAGAA+AGG + Chr4:12301186-12301205 MsG0480018953.01.T01:CDS 40.0%
TCAGAGAGAAGAGGTTGAAT+TGG + Chr4:12301933-12301952 MsG0480018953.01.T01:intron 40.0%
TCAGATCTTTGACTGTTGCA+CGG - Chr4:12304066-12304085 None:intergenic 40.0%
TCAGCATCTCCATTGAACTT+TGG - Chr4:12304188-12304207 None:intergenic 40.0%
TGAGAAAAACACAAGAAGGG+GGG - Chr4:12303998-12304017 None:intergenic 40.0%
TGCATCTATTGGTGAAGTGA+TGG + Chr4:12301801-12301820 MsG0480018953.01.T01:intron 40.0%
TGTGTTTAATCACTTGGCGA+TGG - Chr4:12301441-12301460 None:intergenic 40.0%
TTCAAGGATGTAGTGGCTAA+TGG + Chr4:12301464-12301483 MsG0480018953.01.T01:intron 40.0%
TTGGGAGAGAAACTGGATTA+GGG + Chr4:12301233-12301252 MsG0480018953.01.T01:CDS 40.0%
TTTGGGAGAGAAACTGGATT+AGG + Chr4:12301232-12301251 MsG0480018953.01.T01:CDS 40.0%
! AAATCTCCTTGGAAGTGCAA+GGG + Chr4:12303785-12303804 MsG0480018953.01.T01:intron 40.0%
! AGGATTAGTGGAGTTTGCAA+CGG + Chr4:12303073-12303092 MsG0480018953.01.T01:intron 40.0%
! GATATTCTGGAAGATGGTGT+TGG + Chr4:12304270-12304289 MsG0480018953.01.T01:CDS 40.0%
! GCTACAACAGTGTTGTTCAT+AGG - Chr4:12301265-12301284 None:intergenic 40.0%
! TCAATTGGTATCTAGAGCCT+TGG + Chr4:12304021-12304040 MsG0480018953.01.T01:intron 40.0%
! TCACACAGGTTAGTCATGAA+TGG - Chr4:12301985-12302004 None:intergenic 40.0%
! TCCAGTTGATATTCAGCTTC+TGG - Chr4:12302985-12303004 None:intergenic 40.0%
!! AGGCTCTAGATACCAATTGA+AGG - Chr4:12304021-12304040 None:intergenic 40.0%
!! CACACTTTTCAAGGATGTAG+TGG + Chr4:12301457-12301476 MsG0480018953.01.T01:intron 40.0%
!! TGTTCAGGAGCACTATTCTT+CGG - Chr4:12302843-12302862 None:intergenic 40.0%
!!! CTTTGGAACTTTTCCAACAC+TGG - Chr4:12304171-12304190 None:intergenic 40.0%
AACCGGACTGAAGTATGAGA+AGG + Chr4:12301150-12301169 MsG0480018953.01.T01:CDS 45.0%
AAGTCTCCAAGGAGAACGTT+TGG + Chr4:12301075-12301094 MsG0480018953.01.T01:CDS 45.0%
ACCTGTGTGACTAGGATGTA+TGG + Chr4:12301995-12302014 MsG0480018953.01.T01:intron 45.0%
AGATGGCAATACCCACATGA+TGG - Chr4:12301506-12301525 None:intergenic 45.0%
AGCCTTCTCATACTTCAGTC+CGG - Chr4:12301155-12301174 None:intergenic 45.0%
ATGCTGAAGAGTTCTCTTGG+TGG + Chr4:12304201-12304220 MsG0480018953.01.T01:CDS 45.0%
CATGACTAACCTGTGTGACT+AGG + Chr4:12301987-12302006 MsG0480018953.01.T01:intron 45.0%
CCTGTGTGACTAGGATGTAT+GGG + Chr4:12301996-12302015 MsG0480018953.01.T01:intron 45.0%
GAATAGTGCTCCTGAACAAG+AGG + Chr4:12302845-12302864 MsG0480018953.01.T01:intron 45.0%
GAGATGCTGAAGAGTTCTCT+TGG + Chr4:12304198-12304217 MsG0480018953.01.T01:CDS 45.0%
GCGGAATAAAACAGAGAGAG+AGG - Chr4:12303871-12303890 None:intergenic 45.0%
GGAGTGAAGACCTAGAGAAT+TGG + Chr4:12303405-12303424 MsG0480018953.01.T01:intron 45.0%
GTCAAAAGAGTGGTGAGAAG+AGG + Chr4:12301661-12301680 MsG0480018953.01.T01:CDS 45.0%
TCTTCTCTCTGAACCTCGTA+GGG - Chr4:12301925-12301944 None:intergenic 45.0%
TGAGAAAGGATTGGAGAGCA+TGG + Chr4:12301200-12301219 MsG0480018953.01.T01:CDS 45.0%
TGCTGATGTTTAGCCTGCAA+GGG + Chr4:12301887-12301906 MsG0480018953.01.T01:intron 45.0%
TGCTGGGGTAATGCATCTAT+TGG + Chr4:12301790-12301809 MsG0480018953.01.T01:intron 45.0%
TGGTGAAATGGCTTCTGATG+AGG - Chr4:12302965-12302984 None:intergenic 45.0%
TTGCTGATGTTTAGCCTGCA+AGG + Chr4:12301886-12301905 MsG0480018953.01.T01:intron 45.0%
TTGCTTGTGTGCTTGAGCAT+TGG + Chr4:12303712-12303731 MsG0480018953.01.T01:intron 45.0%
! AAACAGAGTAGTGGAGAACG+TGG - Chr4:12303178-12303197 None:intergenic 45.0%
! AATCTCCTTGGAAGTGCAAG+GGG + Chr4:12303786-12303805 MsG0480018953.01.T01:intron 45.0%
! AGGTTAGTCATGAATGGCAG+TGG - Chr4:12301979-12301998 None:intergenic 45.0%
! ATGATGATGATGAGAGGCAG+TGG + Chr4:12301545-12301564 MsG0480018953.01.T01:intron 45.0%
! ATGTTTGGAGCAAGTCTCCA+AGG + Chr4:12301064-12301083 MsG0480018953.01.T01:CDS 45.0%
! GAAATCTCCTTGGAAGTGCA+AGG + Chr4:12303784-12303803 MsG0480018953.01.T01:intron 45.0%
! GAAGGCGTGTGTTTGCAATT+TGG + Chr4:12303113-12303132 MsG0480018953.01.T01:intron 45.0%
! GAGAGAAACTGGATTAGGGT+GGG + Chr4:12301237-12301256 MsG0480018953.01.T01:CDS 45.0%
! GGCTAATGGAGAGTACACTT+GGG + Chr4:12301478-12301497 MsG0480018953.01.T01:intron 45.0%
! GGTTAGTCATGAATGGCAGT+GGG - Chr4:12301978-12301997 None:intergenic 45.0%
! TGATGCAGCTTCTGATGATG+TGG - Chr4:12302879-12302898 None:intergenic 45.0%
! TGGCTAATGGAGAGTACACT+TGG + Chr4:12301477-12301496 MsG0480018953.01.T01:intron 45.0%
!! GCTGTTTTCTTGTTGTAGCG+CGG - Chr4:12303206-12303225 None:intergenic 45.0%
!! TACTGGTTCCTTCCACAATC+CGG - Chr4:12303825-12303844 None:intergenic 45.0%
!! TTCAGGAGCGCTATTTCTTC+AGG - Chr4:12302810-12302829 None:intergenic 45.0%
ACACTTGGGCACCATCATGT+GGG + Chr4:12301492-12301511 MsG0480018953.01.T01:intron 50.0%
ACTGGACTACTTCCGGATTG+TGG + Chr4:12303810-12303829 MsG0480018953.01.T01:intron 50.0%
ATGCAGGCGAAACCGTATTC+TGG - Chr4:12303230-12303249 None:intergenic 50.0%
CAGCAGCTCCTTCTTCATCA+AGG - Chr4:12304305-12304324 None:intergenic 50.0%
CCCATACATCCTAGTCACAC+AGG - Chr4:12301999-12302018 None:intergenic 50.0%
CGCCTGCATAGATGTTCCTT+TGG + Chr4:12303241-12303260 MsG0480018953.01.T01:intron 50.0%
CTACGAGGTTCAGAGAGAAG+AGG + Chr4:12301924-12301943 MsG0480018953.01.T01:intron 50.0%
CTCGTACCAAACGTTCTCCT+TGG - Chr4:12301084-12301103 None:intergenic 50.0%
CTCTTCTCTCTGAACCTCGT+AGG - Chr4:12301926-12301945 None:intergenic 50.0%
CTCTTGCATTGTGCTTGAGC+AGG + Chr4:12303652-12303671 MsG0480018953.01.T01:intron 50.0%
CTGCTCAAGCACAATGCAAG+AGG - Chr4:12303654-12303673 None:intergenic 50.0%
CTGTTGTAGCACCTGATGAG+TGG + Chr4:12301275-12301294 MsG0480018953.01.T01:CDS 50.0%
GACTACTTCCGGATTGTGGA+AGG + Chr4:12303814-12303833 MsG0480018953.01.T01:intron 50.0%
GATAGCGAGGATGCAAGTGT+TGG + Chr4:12301585-12301604 MsG0480018953.01.T01:CDS 50.0%
GCGGACACAAAACAGAGTAG+TGG - Chr4:12303187-12303206 None:intergenic 50.0%
GTCGTTCTCGCAATGATGCT+GGG + Chr4:12301774-12301793 MsG0480018953.01.T01:intron 50.0%
TACACTTGGGCACCATCATG+TGG + Chr4:12301491-12301510 MsG0480018953.01.T01:intron 50.0%
TCGTTCTCGCAATGATGCTG+GGG + Chr4:12301775-12301794 MsG0480018953.01.T01:intron 50.0%
TCTTCGGAGCAGCTTACTTC+AGG - Chr4:12302827-12302846 None:intergenic 50.0%
TGAAGCTTGGACTCTCCTGA+AGG + Chr4:12302742-12302761 MsG0480018953.01.T01:intron 50.0%
TGATGAAGAAGGAGCTGCTG+TGG + Chr4:12304305-12304324 MsG0480018953.01.T01:CDS 50.0%
TGTAGCACCTGATGAGTGGT+GGG + Chr4:12301279-12301298 MsG0480018953.01.T01:CDS 50.0%
TGTCGTTCTCGCAATGATGC+TGG + Chr4:12301773-12301792 MsG0480018953.01.T01:intron 50.0%
TTCAGGAGCAAGTTGCCTTC+AGG - Chr4:12302760-12302779 None:intergenic 50.0%
TTCAGGAGCAGCTTGACTTC+AGG - Chr4:12302793-12302812 None:intergenic 50.0%
TTGTAGCACCTGATGAGTGG+TGG + Chr4:12301278-12301297 MsG0480018953.01.T01:CDS 50.0%
! ACAACGACTCTTTGGTGTGC+TGG + Chr4:12303346-12303365 MsG0480018953.01.T01:intron 50.0%
! ATCTCCTTGGAAGTGCAAGG+GGG + Chr4:12303787-12303806 MsG0480018953.01.T01:intron 50.0%
! GCTTCTGATGATGTGGCTTC+TGG - Chr4:12302872-12302891 None:intergenic 50.0%
! GGAGAGAAACTGGATTAGGG+TGG + Chr4:12301236-12301255 MsG0480018953.01.T01:CDS 50.0%
!!! CTTCAGGAGCGCTTTTCTTC+AGG - Chr4:12302777-12302796 None:intergenic 50.0%
AAGGGGGACTGGACTACTTC+CGG + Chr4:12303803-12303822 MsG0480018953.01.T01:intron 55.0%
AGAAGGATCTGGCGATAGCG+AGG + Chr4:12301572-12301591 MsG0480018953.01.T01:CDS 55.0%
GATGGCTGAGCTTCACACCA+TGG + Chr4:12301819-12301838 MsG0480018953.01.T01:CDS 55.0%
GCAGTGGCTGTAGAAGGATC+TGG + Chr4:12301561-12301580 MsG0480018953.01.T01:intron 55.0%
GGCTTCTGGACCTCTTGTTC+AGG - Chr4:12302858-12302877 None:intergenic 55.0%
TGAGAGGCAGTGGCTGTAGA+AGG + Chr4:12301555-12301574 MsG0480018953.01.T01:intron 55.0%
TTGGTCTCCCACCACTCATC+AGG - Chr4:12301289-12301308 None:intergenic 55.0%
! GTGGTGGGAGACCAAACAAC+TGG + Chr4:12301294-12301313 MsG0480018953.01.T01:CDS 55.0%
CAGTCCCCCTTGCACTTCCA+AGG - Chr4:12303794-12303813 None:intergenic 60.0%
! CTTGGAAGTGCAAGGGGGAC+TGG + Chr4:12303792-12303811 MsG0480018953.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 12301064 12304353 12301064 ID=MsG0480018953.01;Name=MsG0480018953.01
Chr4 mRNA 12301064 12304353 12301064 ID=MsG0480018953.01.T01;Parent=MsG0480018953.01;Name=MsG0480018953.01.T01;_AED=0.47;_eAED=0.66;_QI=0|0|0|1|0.5|0.8|5|0|269
Chr4 exon 12301064 12301315 12301064 ID=MsG0480018953.01.T01:exon:7526;Parent=MsG0480018953.01.T01
Chr4 exon 12301400 12301472 12301400 ID=MsG0480018953.01.T01:exon:7527;Parent=MsG0480018953.01.T01
Chr4 exon 12301565 12301711 12301565 ID=MsG0480018953.01.T01:exon:7528;Parent=MsG0480018953.01.T01
Chr4 exon 12301818 12301875 12301818 ID=MsG0480018953.01.T01:exon:7529;Parent=MsG0480018953.01.T01
Chr4 exon 12304074 12304353 12304074 ID=MsG0480018953.01.T01:exon:7530;Parent=MsG0480018953.01.T01
Chr4 CDS 12301064 12301315 12301064 ID=MsG0480018953.01.T01:cds;Parent=MsG0480018953.01.T01
Chr4 CDS 12301400 12301472 12301400 ID=MsG0480018953.01.T01:cds;Parent=MsG0480018953.01.T01
Chr4 CDS 12301565 12301711 12301565 ID=MsG0480018953.01.T01:cds;Parent=MsG0480018953.01.T01
Chr4 CDS 12301818 12301875 12301818 ID=MsG0480018953.01.T01:cds;Parent=MsG0480018953.01.T01
Chr4 CDS 12304074 12304353 12304074 ID=MsG0480018953.01.T01:cds;Parent=MsG0480018953.01.T01
Gene Sequence

>MsG0480018953.01.T01

ATGTTTGGAGCAAGTCTCCAAGGAGAACGTTTGGTACGAGCTTTTCAAAAAAAGGTGGACAATATTAGGGTCCGATTTCATAATTTAACCGGACTGAAGTATGAGAAGGCTCAACTAAAGAATAGGTATGACAGTCTGAGAAAGGATTGGAGAGCATGGTATAACTTATTTGGGAGAGAAACTGGATTAGGGTGGGTTCCTATGAACAACACTGTTGTAGCACCTGATGAGTGGTGGGAGACCAAACAACTGGAAAACCCTCTTTATGAAAATTTAGGAACAAGGGGCTTCCATCGCCAAGTGATTAAACACACTTTTCAAGGATTGGCTGTAGAAGGATCTGGCGATAGCGAGGATGCAAGTGTTGGAGCAACAACTGAATTTGGAAATATTGGGTTGAATTCTTCACAAAAAACTGATGGTCAAAAGAGTGGTGAGAAGAGGAAAAGAGCTGTTAGAGCAGACAAGCCAATGATGGCTGAGCTTCACACCATGGATGAAGTCTCAAATGATCTTTATTTGCATGCTCGTCAAAGATCTGAGAAAAACATGACTACTCCTGTTCTGGTTCTAGTGATACTAGCTATAAACATGTCTCACATGATTACAGTGATAGTAAGAAAACCAGTGTTGGAAAAGTTCCAAAGTTCAATGGAGATGCTGAAGAGTTCTCTTGGTGGAAGACCACTTTTACAGTTACATCATGAGCCTGATGAAGAATTATGGGATATTCTGGAAGATGGTGTTGGTGATTTAGTCCTTGATGAAGAAGGAGCTGCTGTGGATAGAAAGAACATACTCCAGAACTGA
Protein sequence

>MsG0480018953.01.T01

MFGASLQGERLVRAFQKKVDNIRVRFHNLTGLKYEKAQLKNRYDSLRKDWRAWYNLFGRETGLGWVPMNNTVVAPDEWWETKQLENPLYENLGTRGFHRQVIKHTFQGLAVEGSGDSEDASVGATTEFGNIGLNSSQKTDGQKSGEKRKRAVRADKPMMAELHTMDEVSNDLYLHARQRSEKNMTTPVLVLVILAINMSHMITVIVRKPVLEKFQSSMEMLKSSLGGRPLLQLHHEPDEELWDILEDGVGDLVLDEEGAAVDRKNILQN*