AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019006.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019006.01.T01 MTR_4g021800 98.137 161 3 0 1 161 1 161 1.04e-115 330
MsG0480019006.01.T01 MTR_4g021800 98.137 161 3 0 1 161 1 161 5.11e-115 330
MsG0480019006.01.T01 MTR_4g021800 90.789 76 6 1 150 225 222 296 7.26e-41 141
MsG0480019006.01.T01 MTR_7g062150 87.578 161 19 1 1 161 1 160 9.27e-101 292
MsG0480019006.01.T01 MTR_7g062150 87.578 161 19 1 1 161 1 160 1.15e-100 294
MsG0480019006.01.T01 MTR_7g062150 77.215 79 14 2 150 225 224 301 3.26e-33 121
MsG0480019006.01.T01 MTR_1g085570 54.237 236 79 2 1 207 1 236 5.37e-89 263
MsG0480019006.01.T01 MTR_1g085570 55.556 225 74 1 9 207 4 228 3.21e-88 261
MsG0480019006.01.T01 MTR_1g085570 55.310 226 74 2 9 207 4 229 1.89e-86 256
MsG0480019006.01.T01 MTR_4g064380 48.659 261 79 3 9 214 8 268 5.44e-83 249
MsG0480019006.01.T01 MTR_4g021825 75.497 151 37 0 11 161 32 182 4.04e-81 245
MsG0480019006.01.T01 MTR_4g021825 74.684 79 16 2 150 225 247 324 1.46e-32 120
MsG0480019006.01.T01 MTR_4g021800 97.414 116 3 0 46 161 1 116 3.45e-79 238
MsG0480019006.01.T01 MTR_4g021800 90.789 76 6 1 150 225 177 251 2.67e-41 141
MsG0480019006.01.T01 MTR_1g085570 53.807 197 65 1 9 179 4 200 5.04e-72 218
MsG0480019006.01.T01 MTR_1g104610 46.696 227 87 2 14 206 8 234 1.97e-67 208
MsG0480019006.01.T01 MTR_2g097520 63.333 150 53 1 1 150 1 148 1.73e-65 204
MsG0480019006.01.T01 MTR_2g097520 52.688 93 39 3 137 225 200 291 3.25e-22 92.4
MsG0480019006.01.T01 MTR_1g104610 48.990 198 69 1 14 179 8 205 2.27e-60 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019006.01.T01 AT5G02230 59.431 281 57 4 1 225 1 280 1.46e-101 296
MsG0480019006.01.T01 AT5G02230 59.431 281 57 4 1 225 1 280 1.46e-101 296
MsG0480019006.01.T01 AT5G02230 59.431 281 57 4 1 225 38 317 2.82e-101 296
MsG0480019006.01.T01 AT5G59480 50.763 262 73 4 1 207 1 261 4.59e-85 254
MsG0480019006.01.T01 AT5G59480 50.763 262 73 4 1 207 1 261 4.59e-85 254
MsG0480019006.01.T01 AT5G59480 50.763 262 73 4 1 207 1 261 4.59e-85 254
MsG0480019006.01.T01 AT5G59480 50.763 262 73 4 1 207 1 261 4.59e-85 254
MsG0480019006.01.T01 AT5G59490 55.983 234 65 1 15 210 10 243 1.29e-83 249
MsG0480019006.01.T01 AT5G59480 50.763 262 72 5 1 207 1 260 5.34e-83 249
MsG0480019006.01.T01 AT3G62040 56.771 192 55 1 46 209 1 192 6.24e-76 228
MsG0480019006.01.T01 AT2G32150 47.788 226 85 1 14 206 4 229 3.51e-73 223
MsG0480019006.01.T01 AT5G59490 53.846 208 58 1 10 179 5 212 7.82e-66 202
MsG0480019006.01.T01 AT2G32150 48.241 199 70 1 14 179 4 202 2.04e-60 189
MsG0480019006.01.T01 AT2G32150 60.432 139 55 0 14 152 4 142 5.10e-58 182

Find 59 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAACTACATTTGAATTT+AGG 0.088582 4:-13223724 None:intergenic
AATGAATAAATTCTCCATTT+TGG 0.164034 4:+13223794 MsG0480019006.01.T01:three_prime_UTR
TGGAAAGCATTCACAATCTT+AGG 0.188813 4:+13223511 MsG0480019006.01.T01:CDS
ATTATTGATCTTAGATTATA+TGG 0.217322 4:+13221917 MsG0480019006.01.T01:intron
ATCAACTACATTTGAATTTA+GGG 0.218826 4:-13223723 None:intergenic
GGGAAGCTGTGCCTGAATTA+TGG 0.237248 4:+13223532 MsG0480019006.01.T01:CDS
TCTGTTCTTCAAAATATTAA+AGG 0.254928 4:+13221674 MsG0480019006.01.T01:CDS
TTTATATAATAATGCCAAAA+TGG 0.270483 4:-13223808 None:intergenic
GAACAACTATGTCTGGTCTA+AGG 0.279798 4:+13222006 MsG0480019006.01.T01:CDS
TTATTAATGAACAATATTAA+AGG 0.284764 4:-13223636 None:intergenic
AAGCCATTAGCTTACCAAAA+AGG 0.285809 4:-13220678 None:intergenic
GGTACATCACAAAGAGTTAA+AGG 0.339752 4:+13223474 MsG0480019006.01.T01:CDS
TATTAATGAACAATATTAAA+GGG 0.359324 4:-13223635 None:intergenic
GGATTAGAAGATTGCTTTGA+AGG 0.372172 4:+13222718 MsG0480019006.01.T01:CDS
GGAAGCTGTGCCTGAATTAT+GGG 0.383288 4:+13223533 MsG0480019006.01.T01:CDS
GATTATATGGTTGAGAAGCT+TGG 0.394134 4:+13221930 MsG0480019006.01.T01:intron
AACCTCCTTTACAAGAACTA+TGG 0.408417 4:+13221984 MsG0480019006.01.T01:CDS
AAAACACTATTCTTGTGGAT+TGG 0.416845 4:-13222766 None:intergenic
GCAACAGATTCCTCAGAATA+GGG 0.431482 4:-13222328 None:intergenic
TGTTAAGGGCAAATGAGAAA+AGG 0.434217 4:-13220641 None:intergenic
AACTATGGAACAACTATGTC+TGG 0.434621 4:+13221999 MsG0480019006.01.T01:CDS
TCTGGTTTCAAGTTCTCATA+AGG 0.450135 4:-13222305 None:intergenic
AGACAAAGTTCATGCTGTTA+AGG 0.455183 4:+13222681 MsG0480019006.01.T01:CDS
GTTAAGGGCAAATGAGAAAA+GGG 0.459001 4:-13220640 None:intergenic
GGAAAGCATTCACAATCTTA+GGG 0.460709 4:+13223512 MsG0480019006.01.T01:CDS
AGCAACAGATTCCTCAGAAT+AGG 0.476120 4:-13222329 None:intergenic
ATGGCGTACGAGGATCGATT+CGG 0.486437 4:+13221475 MsG0480019006.01.T01:CDS
TTAAGGATTTCGGATCGAAA+TGG 0.489486 4:+13221456 MsG0480019006.01.T01:intron
AAGGAAGCAAGAGGAAGAAG+AGG 0.492030 4:-13220576 None:intergenic
TGTTGCTGAGCCTTCCCTAC+AGG 0.493051 4:+13222345 MsG0480019006.01.T01:CDS
GTGACTGATGTTAACTTCCC+AGG 0.494643 4:-13223585 None:intergenic
ATCAGAAAACACTATTCTTG+TGG 0.499713 4:-13222771 None:intergenic
AAGCAATGCTAAGATATTGA+AGG 0.501205 4:-13220595 None:intergenic
GAAGCAAGAGGAAGAAGAGG+GGG 0.507843 4:-13220573 None:intergenic
AGATTCCTCAGAATAGGGTC+TGG 0.510311 4:-13222323 None:intergenic
AGGAAGCAAGAGGAAGAAGA+GGG 0.510485 4:-13220575 None:intergenic
GTTAAGGCACTAAGTAGACT+TGG 0.514037 4:+13222697 MsG0480019006.01.T01:CDS
TTGTTCCATAGTTCTTGTAA+AGG 0.520994 4:-13221989 None:intergenic
ACTTTGTATCCCCTTAGAGC+TGG 0.529127 4:+13221641 MsG0480019006.01.T01:CDS
TTCCATAGTTCTTGTAAAGG+AGG 0.535918 4:-13221986 None:intergenic
AAATCAGAAGTGGCATACCC+TGG 0.536084 4:+13223567 MsG0480019006.01.T01:CDS
AGTGCTTACAAGTTTCCTGT+AGG 0.536657 4:-13222360 None:intergenic
TAAAGGTGCAGATTATGCAT+TGG 0.537919 4:+13223491 MsG0480019006.01.T01:CDS
AACAACTATGTCTGGTCTAA+GGG 0.538656 4:+13222007 MsG0480019006.01.T01:CDS
GGAAGCAAGAGGAAGAAGAG+GGG 0.538727 4:-13220574 None:intergenic
GTGCTTACAAGTTTCCTGTA+GGG 0.557416 4:-13222359 None:intergenic
AGTGTCCGCAATATACAAGC+TGG 0.570089 4:+13223294 MsG0480019006.01.T01:CDS
AATCAGAAGTGGCATACCCT+GGG 0.571637 4:+13223568 MsG0480019006.01.T01:CDS
TTACAAGTTTCCTGTAGGGA+AGG 0.575923 4:-13222355 None:intergenic
TGACTGATGTTAACTTCCCA+GGG 0.594284 4:-13223584 None:intergenic
TGTGGGACTTGACACAGTAT+TGG 0.599786 4:+13223323 MsG0480019006.01.T01:CDS
TCGGATCGAAATGGCGTACG+AGG 0.602969 4:+13221465 MsG0480019006.01.T01:exon
TTGACTATGATGAATATCAC+AGG 0.610280 4:+13222161 MsG0480019006.01.T01:CDS
AAATGACATAAAATCAGAAG+TGG 0.612856 4:+13223557 MsG0480019006.01.T01:CDS
TATACAAGCTGGAAAACGTG+TGG 0.625983 4:+13223305 MsG0480019006.01.T01:CDS
AAGATATTGAAGGAAGCAAG+AGG 0.638493 4:-13220585 None:intergenic
ATACAAGCTGGAAAACGTGT+GGG 0.658378 4:+13223306 MsG0480019006.01.T01:CDS
TGAAACCAGACCCTATTCTG+AGG 0.671934 4:+13222318 MsG0480019006.01.T01:CDS
TTTGCAAGGCCAGCTCTAAG+GGG 0.679893 4:-13221650 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTTTATTATAAAA+TGG + Chr4:13222223-13222242 MsG0480019006.01.T01:intron 0.0%
!! TATTAATGAACAATATTAAA+GGG - Chr4:13223638-13223657 None:intergenic 10.0%
!! TTATTAATGAACAATATTAA+AGG - Chr4:13223639-13223658 None:intergenic 10.0%
!! AACATAAATAAATTTGTGTA+AGG - Chr4:13221201-13221220 None:intergenic 15.0%
!! ATATCAACAATATTAAAGAA+TGG - Chr4:13223189-13223208 None:intergenic 15.0%
!! ATATGTTACAATCAATAAAT+AGG - Chr4:13223447-13223466 None:intergenic 15.0%
!! ATTATTGATCTTAGATTATA+TGG + Chr4:13221917-13221936 MsG0480019006.01.T01:intron 15.0%
!! TCATTCTCTTAAATTATTAT+TGG - Chr4:13221774-13221793 None:intergenic 15.0%
!!! ATATTTTAACATCAGTAAAT+TGG - Chr4:13223078-13223097 None:intergenic 15.0%
!!! TTATTGATTGTAACATATAT+AGG + Chr4:13223449-13223468 MsG0480019006.01.T01:intron 15.0%
!!! TTTTATTTCATTTTCAATTC+AGG + Chr4:13222123-13222142 MsG0480019006.01.T01:intron 15.0%
!! AAACTCAGAGTTAAATATAA+TGG - Chr4:13221067-13221086 None:intergenic 20.0%
!! AAATCTCAAAATCATATTCA+TGG - Chr4:13220814-13220833 None:intergenic 20.0%
!! AACTCAGAGTTAAATATAAT+GGG - Chr4:13221066-13221085 None:intergenic 20.0%
!! ATCAACTACATTTGAATTTA+GGG - Chr4:13223726-13223745 None:intergenic 20.0%
!! ATTAAGAATCATCATTTCTT+TGG + Chr4:13220880-13220899 MsG0480019006.01.T01:intron 20.0%
!! TAAAAAAGGTAAAAGCTATT+TGG - Chr4:13222595-13222614 None:intergenic 20.0%
!! TCTGTTCTTCAAAATATTAA+AGG + Chr4:13221674-13221693 MsG0480019006.01.T01:CDS 20.0%
!!! AATGAATAAATTCTCCATTT+TGG + Chr4:13223794-13223813 MsG0480019006.01.T01:three_prime_UTR 20.0%
!!! ACAAAAAAATTTAAGAGCAT+AGG + Chr4:13223693-13223712 MsG0480019006.01.T01:three_prime_UTR 20.0%
!!! ATAATTTTCAGTTGTTTTTG+AGG + Chr4:13223269-13223288 MsG0480019006.01.T01:intron 20.0%
!!! ATATTTTACAGGAAATGATT+AGG - Chr4:13222055-13222074 None:intergenic 20.0%
!!! ATTATGTTTTTTCAGTGATT+TGG - Chr4:13220769-13220788 None:intergenic 20.0%
!!! CAAAAAAATTTAAGAGCATA+GGG + Chr4:13223694-13223713 MsG0480019006.01.T01:three_prime_UTR 20.0%
!!! GGTATTGATTGTAAATATAA+TGG + Chr4:13222099-13222118 MsG0480019006.01.T01:intron 20.0%
!!! TGTTGAATTTTTAAGGATTT+CGG + Chr4:13221446-13221465 MsG0480019006.01.T01:intron 20.0%
! AAATAACAGTTTGAAAGTGA+AGG - Chr4:13222209-13222228 None:intergenic 25.0%
! AAATGACATAAAATCAGAAG+TGG + Chr4:13223557-13223576 MsG0480019006.01.T01:CDS 25.0%
! AATAACAGTTTGAAAGTGAA+GGG - Chr4:13222208-13222227 None:intergenic 25.0%
! AGGAAATGATTAGGAAATTA+AGG - Chr4:13222046-13222065 None:intergenic 25.0%
! ATATTCATGGTACTAATCAT+AGG - Chr4:13220801-13220820 None:intergenic 25.0%
! ATGTTAAAATATGAATCGTC+TGG + Chr4:13223086-13223105 MsG0480019006.01.T01:intron 25.0%
! ATTGATCGTTTGATAAACTT+TGG - Chr4:13221095-13221114 None:intergenic 25.0%
! CATCAACTACATTTGAATTT+AGG - Chr4:13223727-13223746 None:intergenic 25.0%
! GAAAAATGAGTGATTGTTAA+GGG - Chr4:13220658-13220677 None:intergenic 25.0%
! TCAATTCATCTTTCAAAAGT+TGG + Chr4:13221110-13221129 MsG0480019006.01.T01:intron 25.0%
! TCCATTGTTAAATTGTGAAA+AGG + Chr4:13222078-13222097 MsG0480019006.01.T01:intron 25.0%
! TGATTGTAACATATATAGGT+TGG + Chr4:13223453-13223472 MsG0480019006.01.T01:intron 25.0%
!! AAATATGATTGCCTTCTTTT+TGG + Chr4:13221517-13221536 MsG0480019006.01.T01:CDS 25.0%
!! AATCCAAATCATTTAGGATA+AGG - Chr4:13221249-13221268 None:intergenic 25.0%
!! ACAATGGAAACATATTTTAC+AGG - Chr4:13222066-13222085 None:intergenic 25.0%
!! ACCTTTTCACAATTTAACAA+TGG - Chr4:13222082-13222101 None:intergenic 25.0%
!! ATTTTACTTACTGTTCTTTG+AGG - Chr4:13222984-13223003 None:intergenic 25.0%
!! TGTTCTGTGTTGAATTTTTA+AGG + Chr4:13221439-13221458 MsG0480019006.01.T01:intron 25.0%
!! TTTTACTTACTGTTCTTTGA+GGG - Chr4:13222983-13223002 None:intergenic 25.0%
!!! ATGTTATATGTTGTTTCTTG+AGG + Chr4:13221865-13221884 MsG0480019006.01.T01:intron 25.0%
!!! TTCAAAAGTTGGTTTACTTT+TGG + Chr4:13221121-13221140 MsG0480019006.01.T01:intron 25.0%
!!! TTGCAAATAATTGGTGTTTT+TGG - Chr4:13222912-13222931 None:intergenic 25.0%
!!! TTGTTTGCTAACTTTTTCTA+TGG + Chr4:13223238-13223257 MsG0480019006.01.T01:intron 25.0%
AAAACACTATTCTTGTGGAT+TGG - Chr4:13222769-13222788 None:intergenic 30.0%
AAAACTACTAGACTTGGATT+AGG - Chr4:13222888-13222907 None:intergenic 30.0%
AAGCAATGCTAAGATATTGA+AGG - Chr4:13220598-13220617 None:intergenic 30.0%
AATATAACATGTGTCAGTGT+CGG + Chr4:13221803-13221822 MsG0480019006.01.T01:intron 30.0%
AATTCAATGGCAAATTCTGA+AGG - Chr4:13222936-13222955 None:intergenic 30.0%
ACAAGCGAAACAATTAATAG+AGG - Chr4:13222635-13222654 None:intergenic 30.0%
ACTAACATGGAACAAATGTT+TGG + Chr4:13220856-13220875 MsG0480019006.01.T01:intron 30.0%
ATCAGAAAACACTATTCTTG+TGG - Chr4:13222774-13222793 None:intergenic 30.0%
CATTGAGTAACTAATGTATC+TGG - Chr4:13222522-13222541 None:intergenic 30.0%
CTTCTCAAATTTCACTAACA+TGG + Chr4:13220843-13220862 MsG0480019006.01.T01:intron 30.0%
GGAAAAATGAGTGATTGTTA+AGG - Chr4:13220659-13220678 None:intergenic 30.0%
TCAGAATCTATTCATCAACA+TGG - Chr4:13221359-13221378 None:intergenic 30.0%
TGAGTTGTGACACATTAAAA+AGG - Chr4:13223418-13223437 None:intergenic 30.0%
TTCAGAATTTGCCATTGAAT+TGG + Chr4:13222935-13222954 MsG0480019006.01.T01:intron 30.0%
TTGACTATGATGAATATCAC+AGG + Chr4:13222161-13222180 MsG0480019006.01.T01:CDS 30.0%
TTGTTCCATAGTTCTTGTAA+AGG - Chr4:13221992-13222011 None:intergenic 30.0%
! AAGCCTTATCCTAAATGATT+TGG + Chr4:13221243-13221262 MsG0480019006.01.T01:intron 30.0%
! AAGGATCATTTTGCATCTTT+TGG + Chr4:13221291-13221310 MsG0480019006.01.T01:intron 30.0%
! ACGAGCAAAAACAACTTTTT+AGG + Chr4:13222459-13222478 MsG0480019006.01.T01:intron 30.0%
! TATTTTACAACGATATCCGA+TGG + Chr4:13220957-13220976 MsG0480019006.01.T01:intron 30.0%
! TCATTTTCAATTCAGGCAAT+TGG + Chr4:13222130-13222149 MsG0480019006.01.T01:intron 30.0%
! TCTATTTAAAACAACCATCG+TGG + Chr4:13220740-13220759 MsG0480019006.01.T01:intron 30.0%
! TTTGAAGAACAGATTTTGCA+AGG - Chr4:13221667-13221686 None:intergenic 30.0%
!! ATCACTCATTTTTCCCTTTT+TGG + Chr4:13220664-13220683 MsG0480019006.01.T01:five_prime_UTR 30.0%
!!! ATGTCATTTTCCCATAATTC+AGG - Chr4:13223546-13223565 None:intergenic 30.0%
!!! ATTGTTTGCAGTTTTGTTCA+TGG + Chr4:13222275-13222294 MsG0480019006.01.T01:intron 30.0%
!!! CGTTTTTTCGCTTATAAGAA+GGG + Chr4:13222554-13222573 MsG0480019006.01.T01:intron 30.0%
!!! TCGTTTTTTCGCTTATAAGA+AGG + Chr4:13222553-13222572 MsG0480019006.01.T01:intron 30.0%
AACAACTATGTCTGGTCTAA+GGG + Chr4:13222007-13222026 MsG0480019006.01.T01:CDS 35.0%
AACCTCCTTTACAAGAACTA+TGG + Chr4:13221984-13222003 MsG0480019006.01.T01:CDS 35.0%
AACTATGGAACAACTATGTC+TGG + Chr4:13221999-13222018 MsG0480019006.01.T01:CDS 35.0%
AAGATATTGAAGGAAGCAAG+AGG - Chr4:13220588-13220607 None:intergenic 35.0%
AATCTTGAGAGCCAATTCAA+TGG - Chr4:13222949-13222968 None:intergenic 35.0%
AGACAAAGTTCATGCTGTTA+AGG + Chr4:13222681-13222700 MsG0480019006.01.T01:CDS 35.0%
AGATGCAAAATGATCCTTCA+AGG - Chr4:13221289-13221308 None:intergenic 35.0%
CGTGATATTACGACTATAGT+CGG + Chr4:13223142-13223161 MsG0480019006.01.T01:intron 35.0%
CTGCATAACATTAACTCTCA+TGG - Chr4:13221610-13221629 None:intergenic 35.0%
CTTCAGATTGAATGTCTATC+TGG - Chr4:13221840-13221859 None:intergenic 35.0%
GAAGATGACATTGAGTTTCT+AGG + Chr4:13222797-13222816 MsG0480019006.01.T01:intron 35.0%
GATGCAAAATGATCCTTCAA+GGG - Chr4:13221288-13221307 None:intergenic 35.0%
GATTATATGGTTGAGAAGCT+TGG + Chr4:13221930-13221949 MsG0480019006.01.T01:intron 35.0%
GGATTAGAAGATTGCTTTGA+AGG + Chr4:13222718-13222737 MsG0480019006.01.T01:CDS 35.0%
GGGACTAATCCAAATCATTT+AGG - Chr4:13221255-13221274 None:intergenic 35.0%
GGTACATCACAAAGAGTTAA+AGG + Chr4:13223474-13223493 MsG0480019006.01.T01:CDS 35.0%
GTTAAGGGCAAATGAGAAAA+GGG - Chr4:13220643-13220662 None:intergenic 35.0%
TACAAGCCTTACCAAAAAGA+AGG - Chr4:13221531-13221550 None:intergenic 35.0%
TCTGAAGGCTTGCAAATAAT+TGG - Chr4:13222921-13222940 None:intergenic 35.0%
TGATTTACAATATTCCCCTC+TGG + Chr4:13222428-13222447 MsG0480019006.01.T01:intron 35.0%
TGTTAAGGGCAAATGAGAAA+AGG - Chr4:13220644-13220663 None:intergenic 35.0%
TTAAGGATTTCGGATCGAAA+TGG + Chr4:13221456-13221475 MsG0480019006.01.T01:intron 35.0%
TTCCATAGTTCTTGTAAAGG+AGG - Chr4:13221989-13222008 None:intergenic 35.0%
TTTGGCAAAACTACTAGACT+TGG - Chr4:13222894-13222913 None:intergenic 35.0%
! AAGCCATTAGCTTACCAAAA+AGG - Chr4:13220681-13220700 None:intergenic 35.0%
! AGCCATTAGCTTACCAAAAA+GGG - Chr4:13220680-13220699 None:intergenic 35.0%
! GTTTTCCAGCTTGTATATTG+CGG - Chr4:13223302-13223321 None:intergenic 35.0%
! TAAAGGTGCAGATTATGCAT+TGG + Chr4:13223491-13223510 MsG0480019006.01.T01:CDS 35.0%
! TGTTCTTTGAGGGTTAATGT+TGG - Chr4:13222973-13222992 None:intergenic 35.0%
! TTTTAGCAAAAAAGCCTCGA+GGG - Chr4:13220996-13221015 None:intergenic 35.0%
!! AGTTGGTTTACTTTTGGAAG+TGG + Chr4:13221127-13221146 MsG0480019006.01.T01:intron 35.0%
!! GGAAAGCATTCACAATCTTA+GGG + Chr4:13223512-13223531 MsG0480019006.01.T01:CDS 35.0%
!! TCTGGTTTCAAGTTCTCATA+AGG - Chr4:13222308-13222327 None:intergenic 35.0%
!! TGATTGCCTTCTTTTTGGTA+AGG + Chr4:13221522-13221541 MsG0480019006.01.T01:intron 35.0%
!! TGGAAAGCATTCACAATCTT+AGG + Chr4:13223511-13223530 MsG0480019006.01.T01:CDS 35.0%
!! TTCCCTTTTTGGTAAGCTAA+TGG + Chr4:13220675-13220694 MsG0480019006.01.T01:intron 35.0%
!! TTGGATTAGGTTGAGCAAAA+TGG - Chr4:13222875-13222894 None:intergenic 35.0%
!! TTTTTAGCAAAAAAGCCTCG+AGG - Chr4:13220997-13221016 None:intergenic 35.0%
AGCAACAGATTCCTCAGAAT+AGG - Chr4:13222332-13222351 None:intergenic 40.0%
AGTGCTTACAAGTTTCCTGT+AGG - Chr4:13222363-13222382 None:intergenic 40.0%
ATACAAGCTGGAAAACGTGT+GGG + Chr4:13223306-13223325 MsG0480019006.01.T01:CDS 40.0%
CCTTCAAGGGAGACAATAAA+GGG - Chr4:13221275-13221294 None:intergenic 40.0%
GAACAACTATGTCTGGTCTA+AGG + Chr4:13222006-13222025 MsG0480019006.01.T01:CDS 40.0%
GCAACAGATTCCTCAGAATA+GGG - Chr4:13222331-13222350 None:intergenic 40.0%
GTGCTTACAAGTTTCCTGTA+GGG - Chr4:13222362-13222381 None:intergenic 40.0%
TAAGTCAGAACCCTTATACG+AGG - Chr4:13223113-13223132 None:intergenic 40.0%
TATACAAGCTGGAAAACGTG+TGG + Chr4:13223305-13223324 MsG0480019006.01.T01:CDS 40.0%
TCCTTCAAGGGAGACAATAA+AGG - Chr4:13221276-13221295 None:intergenic 40.0%
TGACTGATGTTAACTTCCCA+GGG - Chr4:13223587-13223606 None:intergenic 40.0%
TTACAAGTTTCCTGTAGGGA+AGG - Chr4:13222358-13222377 None:intergenic 40.0%
! GTTAAGGCACTAAGTAGACT+TGG + Chr4:13222697-13222716 MsG0480019006.01.T01:CDS 40.0%
AAATCAGAAGTGGCATACCC+TGG + Chr4:13223567-13223586 MsG0480019006.01.T01:CDS 45.0%
AAGGAAGCAAGAGGAAGAAG+AGG - Chr4:13220579-13220598 None:intergenic 45.0%
AATCAGAAGTGGCATACCCT+GGG + Chr4:13223568-13223587 MsG0480019006.01.T01:CDS 45.0%
AATCGTCTGGTCCTCGTATA+AGG + Chr4:13223099-13223118 MsG0480019006.01.T01:intron 45.0%
ACTTTGTATCCCCTTAGAGC+TGG + Chr4:13221641-13221660 MsG0480019006.01.T01:CDS 45.0%
AGATTCCTCAGAATAGGGTC+TGG - Chr4:13222326-13222345 None:intergenic 45.0%
AGGAAGCAAGAGGAAGAAGA+GGG - Chr4:13220578-13220597 None:intergenic 45.0%
AGTGTCCGCAATATACAAGC+TGG + Chr4:13223294-13223313 MsG0480019006.01.T01:CDS 45.0%
ATCGTCTGGTCCTCGTATAA+GGG + Chr4:13223100-13223119 MsG0480019006.01.T01:intron 45.0%
CCCTTTATTGTCTCCCTTGA+AGG + Chr4:13221272-13221291 MsG0480019006.01.T01:intron 45.0%
GGAAGCTGTGCCTGAATTAT+GGG + Chr4:13223533-13223552 MsG0480019006.01.T01:CDS 45.0%
GGGACAGAGTTCAAATCCAT+CGG - Chr4:13220976-13220995 None:intergenic 45.0%
GTCGTGTCTGGTATCTTTGT+CGG - Chr4:13221736-13221755 None:intergenic 45.0%
GTGACTGATGTTAACTTCCC+AGG - Chr4:13223588-13223607 None:intergenic 45.0%
TGAAACCAGACCCTATTCTG+AGG + Chr4:13222318-13222337 MsG0480019006.01.T01:CDS 45.0%
TGCTCGTAAATGTGACCAGA+GGG - Chr4:13222446-13222465 None:intergenic 45.0%
TGTGGGACTTGACACAGTAT+TGG + Chr4:13223323-13223342 MsG0480019006.01.T01:CDS 45.0%
TTGCTCGTAAATGTGACCAG+AGG - Chr4:13222447-13222466 None:intergenic 45.0%
! ATTTTGCAAGGCCAGCTCTA+AGG - Chr4:13221655-13221674 None:intergenic 45.0%
! GGCTTTGTGAAGTTTGTGAG+TGG - Chr4:13221228-13221247 None:intergenic 45.0%
!! TTTTGCAAGGCCAGCTCTAA+GGG - Chr4:13221654-13221673 None:intergenic 45.0%
!! AAAAATATAATTAATTAAAG+TGG + Chr4:13220924-13220943 MsG0480019006.01.T01:intron 5.0%
!!! ATAGTTTAAAATTTTAAAAA+AGG - Chr4:13222609-13222628 None:intergenic 5.0%
ATGGCGTACGAGGATCGATT+CGG + Chr4:13221475-13221494 MsG0480019006.01.T01:CDS 50.0%
GAAGCAAGAGGAAGAAGAGG+GGG - Chr4:13220576-13220595 None:intergenic 50.0%
GATTTGAACTCTGTCCCTCG+AGG + Chr4:13220979-13220998 MsG0480019006.01.T01:intron 50.0%
GCTCGTAAATGTGACCAGAG+GGG - Chr4:13222445-13222464 None:intergenic 50.0%
GGAAGCAAGAGGAAGAAGAG+GGG - Chr4:13220577-13220596 None:intergenic 50.0%
GGGAAGCTGTGCCTGAATTA+TGG + Chr4:13223532-13223551 MsG0480019006.01.T01:CDS 50.0%
! CAGTGATTTGGAAGCCACGA+TGG - Chr4:13220757-13220776 None:intergenic 50.0%
!! TTTGCAAGGCCAGCTCTAAG+GGG - Chr4:13221653-13221672 None:intergenic 50.0%
CTGCATGTCAGTGTCGTGTC+TGG - Chr4:13221748-13221767 None:intergenic 55.0%
TCGGATCGAAATGGCGTACG+AGG + Chr4:13221465-13221484 MsG0480019006.01.T01:exon 55.0%
TGTTGCTGAGCCTTCCCTAC+AGG + Chr4:13222345-13222364 MsG0480019006.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 13220573 13223820 13220573 ID=MsG0480019006.01;Name=MsG0480019006.01
Chr4 mRNA 13220573 13223820 13220573 ID=MsG0480019006.01.T01;Parent=MsG0480019006.01;Name=MsG0480019006.01.T01;_AED=0.35;_eAED=0.35;_QI=127|0.87|0.88|1|0.87|0.88|9|206|225
Chr4 exon 13220573 13220685 13220573 ID=MsG0480019006.01.T01:exon:8261;Parent=MsG0480019006.01.T01
Chr4 exon 13221461 13221538 13221461 ID=MsG0480019006.01.T01:exon:8262;Parent=MsG0480019006.01.T01
Chr4 exon 13221630 13221695 13221630 ID=MsG0480019006.01.T01:exon:8263;Parent=MsG0480019006.01.T01
Chr4 exon 13221931 13222028 13221931 ID=MsG0480019006.01.T01:exon:8264;Parent=MsG0480019006.01.T01
Chr4 exon 13222145 13222182 13222145 ID=MsG0480019006.01.T01:exon:8265;Parent=MsG0480019006.01.T01
Chr4 exon 13222286 13222373 13222286 ID=MsG0480019006.01.T01:exon:8266;Parent=MsG0480019006.01.T01
Chr4 exon 13222667 13222781 13222667 ID=MsG0480019006.01.T01:exon:8267;Parent=MsG0480019006.01.T01
Chr4 exon 13223280 13223344 13223280 ID=MsG0480019006.01.T01:exon:8268;Parent=MsG0480019006.01.T01
Chr4 exon 13223471 13223820 13223471 ID=MsG0480019006.01.T01:exon:8269;Parent=MsG0480019006.01.T01
Chr4 five_prime_UTR 13220573 13220685 13220573 ID=MsG0480019006.01.T01:five_prime_utr;Parent=MsG0480019006.01.T01
Chr4 five_prime_UTR 13221461 13221474 13221461 ID=MsG0480019006.01.T01:five_prime_utr;Parent=MsG0480019006.01.T01
Chr4 CDS 13221475 13221538 13221475 ID=MsG0480019006.01.T01:cds;Parent=MsG0480019006.01.T01
Chr4 CDS 13221630 13221695 13221630 ID=MsG0480019006.01.T01:cds;Parent=MsG0480019006.01.T01
Chr4 CDS 13221931 13222028 13221931 ID=MsG0480019006.01.T01:cds;Parent=MsG0480019006.01.T01
Chr4 CDS 13222145 13222182 13222145 ID=MsG0480019006.01.T01:cds;Parent=MsG0480019006.01.T01
Chr4 CDS 13222286 13222373 13222286 ID=MsG0480019006.01.T01:cds;Parent=MsG0480019006.01.T01
Chr4 CDS 13222667 13222781 13222667 ID=MsG0480019006.01.T01:cds;Parent=MsG0480019006.01.T01
Chr4 CDS 13223280 13223344 13223280 ID=MsG0480019006.01.T01:cds;Parent=MsG0480019006.01.T01
Chr4 CDS 13223471 13223614 13223471 ID=MsG0480019006.01.T01:cds;Parent=MsG0480019006.01.T01
Chr4 three_prime_UTR 13223615 13223820 13223615 ID=MsG0480019006.01.T01:three_prime_utr;Parent=MsG0480019006.01.T01
Gene Sequence

>MsG0480019006.01.T01

ATGGCGTACGAGGATCGATTCGGACAACAAGCTCAAAGATCAAAATATGATTGCCTTCTTTTTGATTTAGATGATACTTTGTATCCCCTTAGAGCTGGCCTTGCAAAATCTGTTCTTCAAAATATTAAAGATTATATGGTTGAGAAGCTTGGAATAGACTCAAGCAAAATTGATGACTTGTCAAACCTCCTTTACAAGAACTATGGAACAACTATGTCTGGTCTAAGGGCAATTGGTTATGACTTTGACTATGATGAATATCACAGTTTTGTTCATGGAAGATTACCTTATGAGAACTTGAAACCAGACCCTATTCTGAGGAATCTGTTGCTGAGCCTTCCCTACAGGAAACTTATCTTCACAAATGCAGACAAAGTTCATGCTGTTAAGGCACTAAGTAGACTTGGATTAGAAGATTGCTTTGAAGGAATTATATGCTTTGAGACACTTAATCCAATCCACAAGAATATTGTTTTTGAGGACAGTGTCCGCAATATACAAGCTGGAAAACGTGTGGGACTTGACACAGTATTGGTTGGTACATCACAAAGAGTTAAAGGTGCAGATTATGCATTGGAAAGCATTCACAATCTTAGGGAAGCTGTGCCTGAATTATGGGAAAATGACATAAAATCAGAAGTGGCATACCCTGGGAAGTTAACATCAGTCACAGCTTAA
Protein sequence

>MsG0480019006.01.T01

MAYEDRFGQQAQRSKYDCLLFDLDDTLYPLRAGLAKSVLQNIKDYMVEKLGIDSSKIDDLSNLLYKNYGTTMSGLRAIGYDFDYDEYHSFVHGRLPYENLKPDPILRNLLLSLPYRKLIFTNADKVHAVKALSRLGLEDCFEGIICFETLNPIHKNIVFEDSVRNIQAGKRVGLDTVLVGTSQRVKGADYALESIHNLREAVPELWENDIKSEVAYPGKLTSVTA*