AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019185.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019185.01.T01 MTR_4g024960 77.119 354 65 1 9 362 1 338 0.0 557
MsG0480019185.01.T01 MTR_4g024940 92.771 249 2 1 7 255 4 236 1.04e-165 462
MsG0480019185.01.T01 MTR_0088s0100 50.400 375 151 8 9 362 2 362 1.31e-110 327
MsG0480019185.01.T01 MTR_4g019730 79.070 43 9 0 234 276 51 93 1.10e-16 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019185.01.T01 AT4G08900 81.921 354 45 2 12 362 5 342 0.0 585
MsG0480019185.01.T01 AT4G08870 75.000 360 68 2 9 362 1 344 0.0 552
MsG0480019185.01.T01 AT4G08870 71.533 274 56 2 9 276 1 258 3.56e-141 401

Find 89 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCATCAATTACACGATTT+TGG 0.183005 4:+15605035 None:intergenic
TCAAATTCATCATAGTTATC+AGG 0.221630 4:+15603637 None:intergenic
ATCACTTCTTGGAGTTCCTT+TGG 0.236067 4:-15604785 MsG0480019185.01.T01:CDS
ATTTGATTGCCACTCTAAAA+AGG 0.245577 4:+15602420 None:intergenic
GCTTTGCTCTTTCTCTAATC+AGG 0.250960 4:+15605005 None:intergenic
GTTGCAACTTCATCACTTCT+TGG 0.271009 4:-15604796 MsG0480019185.01.T01:CDS
CTATGTTCGGCGACTCTTGC+AGG 0.289024 4:-15603563 MsG0480019185.01.T01:intron
CATTTGCGCCTCCTCGCATT+AGG 0.296615 4:-15604732 MsG0480019185.01.T01:CDS
CAATCTCGAATTTCTTGAAT+AGG 0.305513 4:+15604021 None:intergenic
TCACTTCTTGGAGTTCCTTT+GGG 0.323593 4:-15604784 MsG0480019185.01.T01:CDS
GGAGAACTTGTGCGTGCTTT+GGG 0.340324 4:-15604826 MsG0480019185.01.T01:CDS
GGGAGAACTTGTGCGTGCTT+TGG 0.359263 4:-15604827 MsG0480019185.01.T01:CDS
TCCGTTAAGACCCTTAGTTT+TGG 0.364007 4:-15603749 MsG0480019185.01.T01:CDS
AATCTCGAATTTCTTGAATA+GGG 0.366222 4:+15604022 None:intergenic
CTCTAGGAAGTGGCGGTCTC+TGG 0.375979 4:+15602917 None:intergenic
CCCAAAACTAAGGGTCTTAA+CGG 0.380269 4:+15603748 None:intergenic
ATTCATGACTATACCTTCTT+CGG 0.391154 4:+15604675 None:intergenic
GTACTTAAGAAATAAATATC+TGG 0.394185 4:+15602456 None:intergenic
GATAACTATGATGAATTTGA+AGG 0.396018 4:-15603634 MsG0480019185.01.T01:CDS
GAGCCGTCTCTGAGAAGCTT+GGG 0.402013 4:-15603693 MsG0480019185.01.T01:CDS
CTTCAAGGTGATGTTGTTGC+TGG 0.413690 4:-15602596 MsG0480019185.01.T01:CDS
GATTTACTTGTGCAGAAACT+AGG 0.423851 4:-15602746 MsG0480019185.01.T01:intron
ATTTGCGCCTCCTCGCATTA+GGG 0.439031 4:-15604731 MsG0480019185.01.T01:CDS
CTCGCATTAGGGAAGCCATT+TGG 0.440207 4:-15604720 MsG0480019185.01.T01:CDS
ATGCGAGGAGGCGCAAATGC+AGG 0.453201 4:+15604736 None:intergenic
CTCAGAGACGGCTCTAATAA+CGG 0.453596 4:+15603702 None:intergenic
CATCGACAGTATCGCGTTGT+GGG 0.457317 4:+15602555 None:intergenic
AACATCCTACACAATCTTCA+AGG 0.471336 4:-15602611 MsG0480019185.01.T01:CDS
TGTAGAAATTGCTTCTCTGC+AGG 0.473547 4:-15604848 MsG0480019185.01.T01:CDS
TGCTAAGTTGGTGAGAGAAT+TGG 0.476269 4:-15602513 MsG0480019185.01.T01:CDS
GTGATCACCACCCAAAACTA+AGG 0.477869 4:+15603738 None:intergenic
TCAGAGACGGCTCTAATAAC+GGG 0.479420 4:+15603703 None:intergenic
GCGCAAATGCAGGCCCTTGA+AGG 0.482281 4:+15604746 None:intergenic
AGAGACATACCAGGTTCTCT+AGG 0.483921 4:+15602901 None:intergenic
GTTTGTACTACCACACCAAA+TGG 0.486647 4:+15604705 None:intergenic
TTACATGCAGAGACTGAATT+CGG 0.487466 4:-15605089 MsG0480019185.01.T01:CDS
TAGAGAAAGAGCAAAGCTTA+AGG 0.487906 4:-15604999 MsG0480019185.01.T01:CDS
TGGTTCTATGTGAGACACTC+CGG 0.492904 4:+15602661 None:intergenic
TTCTTCCTTTGCTCGAGTCA+TGG 0.493089 4:-15603593 MsG0480019185.01.T01:CDS
AATTCAGTCTCTGCATGTAA+TGG 0.495789 4:+15605092 None:intergenic
CTTTGCTCTTTCTCTAATCA+GGG 0.500708 4:+15605006 None:intergenic
ATTTACTTGTGCAGAAACTA+GGG 0.501074 4:-15602745 MsG0480019185.01.T01:intron
TCATGGAGGGTAACTATGTT+CGG 0.503666 4:-15603576 MsG0480019185.01.T01:CDS
ATTTATTTCTTAAGTACGCT+TGG 0.509725 4:-15602450 MsG0480019185.01.T01:three_prime_UTR
CATAATTCATCATTCCTTCA+AGG 0.510490 4:-15604760 MsG0480019185.01.T01:CDS
TCATCTGCTTTGCTAGAGAA+TGG 0.515301 4:-15605058 MsG0480019185.01.T01:CDS
CAAATTCATCATAGTTATCA+GGG 0.516922 4:+15603638 None:intergenic
CCATCGACAGTATCGCGTTG+TGG 0.523089 4:+15602554 None:intergenic
TCCTTTGCTCGAGTCATGGA+GGG 0.526222 4:-15603589 MsG0480019185.01.T01:CDS
CCGCCACTTCCTAGAGAACC+TGG 0.528589 4:-15602910 MsG0480019185.01.T01:intron
GACACTCCGGGAGCAAAAGC+AGG 0.531124 4:+15602674 None:intergenic
GGTTCTATGTGAGACACTCC+GGG 0.532823 4:+15602662 None:intergenic
ATTCAAGAAATTCGAGATTG+TGG 0.534207 4:-15604018 MsG0480019185.01.T01:CDS
TGCTATGGTAGCTGCTAAGT+TGG 0.538105 4:-15602525 MsG0480019185.01.T01:CDS
ATAATTCATCATTCCTTCAA+GGG 0.538757 4:-15604759 MsG0480019185.01.T01:CDS
ATTTCTTAAGTACGCTTGGA+AGG 0.540668 4:-15602446 MsG0480019185.01.T01:three_prime_UTR
ACAAACTCGACAACCGAAGA+AGG 0.545861 4:-15604688 MsG0480019185.01.T01:intron
CATAGATTGATGAATGTCAT+TGG 0.546830 4:-15603985 MsG0480019185.01.T01:CDS
TGATGTTGTTGCTGGAGATG+TGG 0.553198 4:-15602588 MsG0480019185.01.T01:CDS
AGAGCCGTCTCTGAGAAGCT+TGG 0.556351 4:-15603694 MsG0480019185.01.T01:CDS
ATACCAGGTTCTCTAGGAAG+TGG 0.566389 4:+15602907 None:intergenic
TCGCGGAAAGAAAGACCTCC+TGG 0.571109 4:+15602641 None:intergenic
AAACTAGGGGAAGGTGTGAA+AGG 0.572287 4:-15602731 MsG0480019185.01.T01:CDS
GTAGAAATTGCTTCTCTGCA+GGG 0.575454 4:-15604847 MsG0480019185.01.T01:CDS
ATTCGTTCAATAACAACTGA+AGG 0.575794 4:-15602993 MsG0480019185.01.T01:CDS
TGTATATATCTCAATAGATG+TGG 0.586320 4:-15602708 MsG0480019185.01.T01:CDS
ACCCTCCATGACTCGAGCAA+AGG 0.586892 4:+15603588 None:intergenic
TGTCAAGTTAGTGATGGAAG+AGG 0.598921 4:-15603956 MsG0480019185.01.T01:intron
TTCCTTTGCTCGAGTCATGG+AGG 0.602924 4:-15603590 MsG0480019185.01.T01:CDS
AATGATGAATTATGACCCAA+AGG 0.605823 4:+15604769 None:intergenic
AGCCGTCTCTGAGAAGCTTG+GGG 0.614679 4:-15603692 MsG0480019185.01.T01:CDS
GAACTTGTGCGTGCTTTGGG+AGG 0.623278 4:-15604823 MsG0480019185.01.T01:CDS
GCACGAGTGCTAACTGATGT+TGG 0.626280 4:-15604051 MsG0480019185.01.T01:CDS
TGATCACCACCCAAAACTAA+GGG 0.632083 4:+15603739 None:intergenic
TGAATCTGTCAAGTTAGTGA+TGG 0.632395 4:-15603962 MsG0480019185.01.T01:CDS
CCAGGTTCTCTAGGAAGTGG+CGG 0.634049 4:+15602910 None:intergenic
CATCACCTTGAAGATTGTGT+AGG 0.638689 4:+15602606 None:intergenic
ATTAGGGAAGCCATTTGGTG+TGG 0.639742 4:-15604715 MsG0480019185.01.T01:CDS
TGTCGATGGAATGACTGCTA+TGG 0.640459 4:-15602540 MsG0480019185.01.T01:CDS
GGAGTGTCTCACATAGAACC+AGG 0.643921 4:-15602659 MsG0480019185.01.T01:CDS
CCACAACGCGATACTGTCGA+TGG 0.646892 4:-15602554 MsG0480019185.01.T01:CDS
CTTGTGCAGAAACTAGGGGA+AGG 0.662379 4:-15602740 MsG0480019185.01.T01:intron
GCCGTCTCTGAGAAGCTTGG+GGG 0.674478 4:-15603691 MsG0480019185.01.T01:CDS
CAAATGGCTTCCCTAATGCG+AGG 0.684146 4:+15604721 None:intergenic
TTTACTTGTGCAGAAACTAG+GGG 0.686309 4:-15602744 MsG0480019185.01.T01:intron
ATGTCGACGATAGCACGTAG+AGG 0.716506 4:-15605118 MsG0480019185.01.T01:CDS
ATGGCTTCCCTAATGCGAGG+AGG 0.726925 4:+15604724 None:intergenic
GTGTCTCACATAGAACCAGG+AGG 0.775201 4:-15602656 MsG0480019185.01.T01:CDS
GTAGGATGTTAAGAACATCG+CGG 0.825273 4:+15602624 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTGTTAATTTATTTC+AGG - Chr4:15604448-15604467 MsG0480019185.01.T01:intron 10.0%
!!! AAAAATGATTTTTTTTGTGT+AGG - Chr4:15602840-15602859 MsG0480019185.01.T01:intron 15.0%
!! AAGCAAAATATTGTACAAAA+TGG - Chr4:15604637-15604656 MsG0480019185.01.T01:intron 20.0%
!! AATATAAAACTACAGGTTAA+AGG + Chr4:15602554-15602573 None:intergenic 20.0%
!! ATACTTTGTCATTAAGTTAT+GGG - Chr4:15604211-15604230 MsG0480019185.01.T01:intron 20.0%
!! ATTAACAAATTAAAGCATGA+AGG + Chr4:15604441-15604460 None:intergenic 20.0%
!! CAAATGAAATATAAAACTAC+AGG + Chr4:15602561-15602580 None:intergenic 20.0%
!! CTGAAAAATCAAATAATTAC+AGG + Chr4:15603381-15603400 None:intergenic 20.0%
!! GTACTTAAGAAATAAATATC+TGG + Chr4:15605016-15605035 None:intergenic 20.0%
!! TATAAAATTAAGTGCATGTT+TGG + Chr4:15604119-15604138 None:intergenic 20.0%
!! TATACTTTGTCATTAAGTTA+TGG - Chr4:15604210-15604229 MsG0480019185.01.T01:intron 20.0%
!!! ATATTTCATTTGGATGATTT+TGG - Chr4:15602569-15602588 MsG0480019185.01.T01:CDS 20.0%
!!! ATTATTTGATTTTTCAGGTA+AGG - Chr4:15603384-15603403 MsG0480019185.01.T01:intron 20.0%
!!! CTGTAATTATTTGATTTTTC+AGG - Chr4:15603379-15603398 MsG0480019185.01.T01:intron 20.0%
!!! CTGTAGTTTTATATTTCATT+TGG - Chr4:15602559-15602578 MsG0480019185.01.T01:CDS 20.0%
!!! TATTATGTTGTTTTGAGATT+CGG - Chr4:15604670-15604689 MsG0480019185.01.T01:intron 20.0%
!!! TTTGTTAATTTATTTCAGGT+TGG - Chr4:15604452-15604471 MsG0480019185.01.T01:intron 20.0%
! AACCAAATATTGTCAAATAG+CGG + Chr4:15603164-15603183 None:intergenic 25.0%
! AATACATATGTCGATATCAT+TGG - Chr4:15602946-15602965 MsG0480019185.01.T01:CDS 25.0%
! AATCTCGAATTTCTTGAATA+GGG + Chr4:15603450-15603469 None:intergenic 25.0%
! AGACATACATTAAACTGTTT+AGG + Chr4:15603975-15603994 None:intergenic 25.0%
! ATAATTCATCATTCCTTCAA+GGG - Chr4:15602710-15602729 MsG0480019185.01.T01:CDS 25.0%
! CAAATTCATCATAGTTATCA+GGG + Chr4:15603834-15603853 None:intergenic 25.0%
! GATAACTATGATGAATTTGA+AGG - Chr4:15603835-15603854 MsG0480019185.01.T01:intron 25.0%
! TAAACAGTTTAATGTATGTC+TGG - Chr4:15603974-15603993 MsG0480019185.01.T01:CDS 25.0%
! TCAAATTCATCATAGTTATC+AGG + Chr4:15603835-15603854 None:intergenic 25.0%
! TGTATATATCTCAATAGATG+TGG - Chr4:15604761-15604780 MsG0480019185.01.T01:CDS 25.0%
! TTATTCCTTGTATGAAATCT+AGG - Chr4:15602906-15602925 MsG0480019185.01.T01:intron 25.0%
!! ATGAAGGTGTATATGTTTAT+AGG + Chr4:15603594-15603613 None:intergenic 25.0%
!! ATTTATTTCTTAAGTACGCT+TGG - Chr4:15605019-15605038 MsG0480019185.01.T01:CDS 25.0%
!! TGAAGGTGTATATGTTTATA+GGG + Chr4:15603593-15603612 None:intergenic 25.0%
!! TGAATTTTCCAAAATCATGA+CGG - Chr4:15604005-15604024 MsG0480019185.01.T01:CDS 25.0%
AATGAAACACAGATTCAAAC+AGG + Chr4:15602521-15602540 None:intergenic 30.0%
AATGATGAATTATGACCCAA+AGG + Chr4:15602703-15602722 None:intergenic 30.0%
ATGACATATCTTACAAACCA+TGG + Chr4:15604414-15604433 None:intergenic 30.0%
ATTCAAGAAATTCGAGATTG+TGG - Chr4:15603451-15603470 MsG0480019185.01.T01:intron 30.0%
ATTCATGACTATACCTTCTT+CGG + Chr4:15602797-15602816 None:intergenic 30.0%
ATTCGTTCAATAACAACTGA+AGG - Chr4:15604476-15604495 MsG0480019185.01.T01:intron 30.0%
ATTTACTTGTGCAGAAACTA+GGG - Chr4:15604724-15604743 MsG0480019185.01.T01:CDS 30.0%
CAATATATTCAGCATGATGA+AGG + Chr4:15603610-15603629 None:intergenic 30.0%
CAATCTCGAATTTCTTGAAT+AGG + Chr4:15603451-15603470 None:intergenic 30.0%
CATAATTCATCATTCCTTCA+AGG - Chr4:15602709-15602728 MsG0480019185.01.T01:CDS 30.0%
CATAGATTGATGAATGTCAT+TGG - Chr4:15603484-15603503 MsG0480019185.01.T01:intron 30.0%
GTATAGTCATGAATTTCCTT+AGG - Chr4:15602803-15602822 MsG0480019185.01.T01:intron 30.0%
TACTACCTAGATTTCATACA+AGG + Chr4:15602914-15602933 None:intergenic 30.0%
TATGAAATCTAGGTAGTAGA+AGG - Chr4:15602916-15602935 MsG0480019185.01.T01:CDS 30.0%
TATTAAAAAGCAACGCTACA+TGG + Chr4:15603234-15603253 None:intergenic 30.0%
TGACATATCTTACAAACCAT+GGG + Chr4:15604413-15604432 None:intergenic 30.0%
TGAGTTTGACAAAATAACGA+CGG - Chr4:15604083-15604102 MsG0480019185.01.T01:intron 30.0%
TGTTATTACAGAGAACAACT+AGG + Chr4:15604162-15604181 None:intergenic 30.0%
TTAAGACTTCTACAATCCTA+AGG + Chr4:15602822-15602841 None:intergenic 30.0%
! AAGCATCAATTACACGATTT+TGG + Chr4:15602437-15602456 None:intergenic 30.0%
! AGTGGCAATCAAATTTTACT+AGG - Chr4:15605058-15605077 MsG0480019185.01.T01:CDS 30.0%
! ATTTGATTGCCACTCTAAAA+AGG + Chr4:15605052-15605071 None:intergenic 30.0%
! TTGTATTTCTGAGGATAACA+TGG - Chr4:15603023-15603042 MsG0480019185.01.T01:intron 30.0%
!!! TTTTTCAGGTAAGGATTTAC+AGG - Chr4:15603393-15603412 MsG0480019185.01.T01:intron 30.0%
AACATCCTACACAATCTTCA+AGG - Chr4:15604858-15604877 MsG0480019185.01.T01:CDS 35.0%
AATTCAGTCTCTGCATGTAA+TGG + Chr4:15602380-15602399 None:intergenic 35.0%
ACACGAGCAACTTATTTAAC+AGG - Chr4:15603296-15603315 MsG0480019185.01.T01:intron 35.0%
ATCATTGGATTTGCGTTGTT+AGG - Chr4:15602961-15602980 MsG0480019185.01.T01:CDS 35.0%
ATTTCTTAAGTACGCTTGGA+AGG - Chr4:15605023-15605042 MsG0480019185.01.T01:CDS 35.0%
CTTTGCTCTTTCTCTAATCA+GGG + Chr4:15602466-15602485 None:intergenic 35.0%
GACAACACTGAATAGAATAC+AGG + Chr4:15603072-15603091 None:intergenic 35.0%
GAGGACATAAAACATGTCTA+CGG + Chr4:15602997-15603016 None:intergenic 35.0%
GATTTACTTGTGCAGAAACT+AGG - Chr4:15604723-15604742 MsG0480019185.01.T01:CDS 35.0%
GCTTAAGGCAAGTCATTATT+TGG - Chr4:15602485-15602504 MsG0480019185.01.T01:exon 35.0%
TAGAGAAAGAGCAAAGCTTA+AGG - Chr4:15602470-15602489 MsG0480019185.01.T01:three_prime_UTR 35.0%
TATCTGCACTAAACGAAAAG+CGG + Chr4:15604277-15604296 None:intergenic 35.0%
TGAATAGAATACAGGTGAAC+AGG + Chr4:15603064-15603083 None:intergenic 35.0%
TGAATCTGTCAAGTTAGTGA+TGG - Chr4:15603507-15603526 MsG0480019185.01.T01:intron 35.0%
TGCCGCTATTTGACAATATT+TGG - Chr4:15603159-15603178 MsG0480019185.01.T01:intron 35.0%
TTACATGCAGAGACTGAATT+CGG - Chr4:15602380-15602399 MsG0480019185.01.T01:three_prime_UTR 35.0%
TTGTCATTAAGTTATGGGAG+TGG - Chr4:15604216-15604235 MsG0480019185.01.T01:intron 35.0%
TTTACTTGTGCAGAAACTAG+GGG - Chr4:15604725-15604744 MsG0480019185.01.T01:CDS 35.0%
! ACATGTTTTATGTCCTCTTG+TGG - Chr4:15603000-15603019 MsG0480019185.01.T01:CDS 35.0%
! CTTTTCGTTTAGTGCAGATA+AGG - Chr4:15604277-15604296 MsG0480019185.01.T01:intron 35.0%
! TATCAATACAAGAAGCGTGA+CGG + Chr4:15603352-15603371 None:intergenic 35.0%
! TGTAGCTTTTGCCAAAATTG+TGG - Chr4:15604055-15604074 MsG0480019185.01.T01:CDS 35.0%
!! ATTGGTGTTTTTCTGAACCT+TGG - Chr4:15604699-15604718 MsG0480019185.01.T01:CDS 35.0%
ATCACTTCTTGGAGTTCCTT+TGG - Chr4:15602684-15602703 MsG0480019185.01.T01:CDS 40.0%
CATCACCTTGAAGATTGTGT+AGG + Chr4:15604866-15604885 None:intergenic 40.0%
CCCAAAACTAAGGGTCTTAA+CGG + Chr4:15603724-15603743 None:intergenic 40.0%
CTCAGAAATACAACCACAAG+AGG + Chr4:15603016-15603035 None:intergenic 40.0%
CTCATCAGAAGCTCACAAAT+TGG + Chr4:15603549-15603568 None:intergenic 40.0%
GCTTTGCTCTTTCTCTAATC+AGG + Chr4:15602467-15602486 None:intergenic 40.0%
GTAGAAATTGCTTCTCTGCA+GGG - Chr4:15602622-15602641 MsG0480019185.01.T01:CDS 40.0%
GTAGGATGTTAAGAACATCG+CGG + Chr4:15604848-15604867 None:intergenic 40.0%
GTGCGCAAGCAAAAAAGTTT+GGG - Chr4:15604501-15604520 MsG0480019185.01.T01:intron 40.0%
GTTGCAACTTCATCACTTCT+TGG - Chr4:15602673-15602692 MsG0480019185.01.T01:CDS 40.0%
GTTTGTACTACCACACCAAA+TGG + Chr4:15602767-15602786 None:intergenic 40.0%
TAAATGTCAGTCTTCACCCA+TGG - Chr4:15604394-15604413 MsG0480019185.01.T01:intron 40.0%
TCACTTCTTGGAGTTCCTTT+GGG - Chr4:15602685-15602704 MsG0480019185.01.T01:CDS 40.0%
TCATCTGCTTTGCTAGAGAA+TGG - Chr4:15602411-15602430 MsG0480019185.01.T01:three_prime_UTR 40.0%
TCATGGAGGGTAACTATGTT+CGG - Chr4:15603893-15603912 MsG0480019185.01.T01:intron 40.0%
TCTGCACAAGTAAATCACCA+AGG + Chr4:15604719-15604738 None:intergenic 40.0%
TGATCACCACCCAAAACTAA+GGG + Chr4:15603733-15603752 None:intergenic 40.0%
TGCGCAAGCAAAAAAGTTTG+GGG - Chr4:15604502-15604521 MsG0480019185.01.T01:intron 40.0%
TGCTTGATCCACATTCTTTC+AGG - Chr4:15603696-15603715 MsG0480019185.01.T01:CDS 40.0%
TGTAGAAATTGCTTCTCTGC+AGG - Chr4:15602621-15602640 MsG0480019185.01.T01:CDS 40.0%
TTGTCAAATAGCGGCAACAA+CGG + Chr4:15603155-15603174 None:intergenic 40.0%
! AACTCAAGCCACCACAATTT+TGG + Chr4:15604069-15604088 None:intergenic 40.0%
! AGCTTTTGCCAAAATTGTGG+TGG - Chr4:15604058-15604077 MsG0480019185.01.T01:CDS 40.0%
! CTCTTGTGGTTGTATTTCTG+AGG - Chr4:15603014-15603033 MsG0480019185.01.T01:intron 40.0%
! TAACGGATCCTGAAAGAATG+TGG + Chr4:15603707-15603726 None:intergenic 40.0%
! TCCGTTAAGACCCTTAGTTT+TGG - Chr4:15603720-15603739 MsG0480019185.01.T01:CDS 40.0%
! TGTCAAGTTAGTGATGGAAG+AGG - Chr4:15603513-15603532 MsG0480019185.01.T01:intron 40.0%
!! CCGTTAAGACCCTTAGTTTT+GGG - Chr4:15603721-15603740 MsG0480019185.01.T01:CDS 40.0%
!! GGAAGGATACCTTTTTAGAG+TGG - Chr4:15605040-15605059 MsG0480019185.01.T01:CDS 40.0%
!! TGCTAAGTTGGTGAGAGAAT+TGG - Chr4:15604956-15604975 MsG0480019185.01.T01:intron 40.0%
!! TTTGAGATTCGGTGCTTCAT+TGG - Chr4:15604681-15604700 MsG0480019185.01.T01:intron 40.0%
!!! TTAAGACCCTTAGTTTTGGG+TGG - Chr4:15603724-15603743 MsG0480019185.01.T01:CDS 40.0%
ACAAACTCGACAACCGAAGA+AGG - Chr4:15602781-15602800 MsG0480019185.01.T01:intron 45.0%
AGAGACATACCAGGTTCTCT+AGG + Chr4:15604571-15604590 None:intergenic 45.0%
CGCTATGGCACTATTGTATG+TGG + Chr4:15603132-15603151 None:intergenic 45.0%
CGTGCGCAAGCAAAAAAGTT+TGG - Chr4:15604500-15604519 MsG0480019185.01.T01:intron 45.0%
CTGCAGATCGTAGGAAATAG+CGG - Chr4:15603094-15603113 MsG0480019185.01.T01:intron 45.0%
CTTCAAGGTGATGTTGTTGC+TGG - Chr4:15604873-15604892 MsG0480019185.01.T01:CDS 45.0%
GTGATCACCACCCAAAACTA+AGG + Chr4:15603734-15603753 None:intergenic 45.0%
GTGTGCGTGTGTATATGCTT+GGG - Chr4:15604609-15604628 MsG0480019185.01.T01:intron 45.0%
TGATGTTGTTGCTGGAGATG+TGG - Chr4:15604881-15604900 MsG0480019185.01.T01:intron 45.0%
TGCTATGGTAGCTGCTAAGT+TGG - Chr4:15604944-15604963 MsG0480019185.01.T01:intron 45.0%
TGTCGATGGAATGACTGCTA+TGG - Chr4:15604929-15604948 MsG0480019185.01.T01:intron 45.0%
TGTTGTCAACTGCAGATCGT+AGG - Chr4:15603085-15603104 MsG0480019185.01.T01:intron 45.0%
TTCTTCCTTTGCTCGAGTCA+TGG - Chr4:15603876-15603895 MsG0480019185.01.T01:intron 45.0%
! AAACTAGGGGAAGGTGTGAA+AGG - Chr4:15604738-15604757 MsG0480019185.01.T01:CDS 45.0%
! ATACCAGGTTCTCTAGGAAG+TGG + Chr4:15604565-15604584 None:intergenic 45.0%
! ATTAGGGAAGCCATTTGGTG+TGG - Chr4:15602754-15602773 MsG0480019185.01.T01:intron 45.0%
! CTCAGAGACGGCTCTAATAA+CGG + Chr4:15603770-15603789 None:intergenic 45.0%
! TCACAGTGCCGTCATGATTT+TGG + Chr4:15604016-15604035 None:intergenic 45.0%
! TCAGAGACGGCTCTAATAAC+GGG + Chr4:15603769-15603788 None:intergenic 45.0%
! TGGTTCTATGTGAGACACTC+CGG + Chr4:15604811-15604830 None:intergenic 45.0%
ATGTCGACGATAGCACGTAG+AGG - Chr4:15602351-15602370 MsG0480019185.01.T01:three_prime_UTR 50.0%
ATTTGCGCCTCCTCGCATTA+GGG - Chr4:15602738-15602757 MsG0480019185.01.T01:intron 50.0%
CAAATGGCTTCCCTAATGCG+AGG + Chr4:15602751-15602770 None:intergenic 50.0%
CATCGACAGTATCGCGTTGT+GGG + Chr4:15604917-15604936 None:intergenic 50.0%
CGTGTGCGTGTGTATATGCT+TGG - Chr4:15604608-15604627 MsG0480019185.01.T01:intron 50.0%
CTCGCATTAGGGAAGCCATT+TGG - Chr4:15602749-15602768 MsG0480019185.01.T01:intron 50.0%
CTTGTGCAGAAACTAGGGGA+AGG - Chr4:15604729-15604748 MsG0480019185.01.T01:CDS 50.0%
GCACGAGTGCTAACTGATGT+TGG - Chr4:15603418-15603437 MsG0480019185.01.T01:intron 50.0%
GGAGAACTTGTGCGTGCTTT+GGG - Chr4:15602643-15602662 MsG0480019185.01.T01:CDS 50.0%
GGTTCTATGTGAGACACTCC+GGG + Chr4:15604810-15604829 None:intergenic 50.0%
GTGTCTCACATAGAACCAGG+AGG - Chr4:15604813-15604832 MsG0480019185.01.T01:CDS 50.0%
TCCTTTGCTCGAGTCATGGA+GGG - Chr4:15603880-15603899 MsG0480019185.01.T01:intron 50.0%
TTCCTTTGCTCGAGTCATGG+AGG - Chr4:15603879-15603898 MsG0480019185.01.T01:intron 50.0%
! GGAGTGTCTCACATAGAACC+AGG - Chr4:15604810-15604829 MsG0480019185.01.T01:CDS 50.0%
!! CTTGATCCTGCTTTTGCTCC+CGG - Chr4:15604789-15604808 MsG0480019185.01.T01:CDS 50.0%
ACCCTCCATGACTCGAGCAA+AGG + Chr4:15603884-15603903 None:intergenic 55.0%
ATGGCTTCCCTAATGCGAGG+AGG + Chr4:15602748-15602767 None:intergenic 55.0%
CATTTGCGCCTCCTCGCATT+AGG - Chr4:15602737-15602756 MsG0480019185.01.T01:intron 55.0%
CCACAACGCGATACTGTCGA+TGG - Chr4:15604915-15604934 MsG0480019185.01.T01:intron 55.0%
CCATCGACAGTATCGCGTTG+TGG + Chr4:15604918-15604937 None:intergenic 55.0%
CTATGTTCGGCGACTCTTGC+AGG - Chr4:15603906-15603925 MsG0480019185.01.T01:intron 55.0%
GCGACAGAGAGAGACATACC+AGG + Chr4:15604580-15604599 None:intergenic 55.0%
GGGAGAACTTGTGCGTGCTT+TGG - Chr4:15602642-15602661 MsG0480019185.01.T01:CDS 55.0%
TCCCCCAAGCTTCTCAGAGA+CGG + Chr4:15603782-15603801 None:intergenic 55.0%
TCGCGGAAAGAAAGACCTCC+TGG + Chr4:15604831-15604850 None:intergenic 55.0%
! AGAGCCGTCTCTGAGAAGCT+TGG - Chr4:15603775-15603794 MsG0480019185.01.T01:intron 55.0%
! AGCCGTCTCTGAGAAGCTTG+GGG - Chr4:15603777-15603796 MsG0480019185.01.T01:intron 55.0%
! CCAGGTTCTCTAGGAAGTGG+CGG + Chr4:15604562-15604581 None:intergenic 55.0%
! GAACTTGTGCGTGCTTTGGG+AGG - Chr4:15602646-15602665 MsG0480019185.01.T01:CDS 55.0%
! GAGCCGTCTCTGAGAAGCTT+GGG - Chr4:15603776-15603795 MsG0480019185.01.T01:intron 55.0%
CCGCCACTTCCTAGAGAACC+TGG - Chr4:15604559-15604578 MsG0480019185.01.T01:intron 60.0%
GACACTCCGGGAGCAAAAGC+AGG + Chr4:15604798-15604817 None:intergenic 60.0%
GCGCAAATGCAGGCCCTTGA+AGG + Chr4:15602726-15602745 None:intergenic 60.0%
TAGCGGCAACAACGGCGCTA+TGG + Chr4:15603147-15603166 None:intergenic 60.0%
! ATGCGAGGAGGCGCAAATGC+AGG + Chr4:15602736-15602755 None:intergenic 60.0%
! CTCTAGGAAGTGGCGGTCTC+TGG + Chr4:15604555-15604574 None:intergenic 60.0%
! CTCTGAGAAGCTTGGGGGAC+TGG - Chr4:15603783-15603802 MsG0480019185.01.T01:intron 60.0%
! GCCGTCTCTGAGAAGCTTGG+GGG - Chr4:15603778-15603797 MsG0480019185.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 15602327 15605164 15602327 ID=MsG0480019185.01;Name=MsG0480019185.01
Chr4 mRNA 15602327 15605164 15602327 ID=MsG0480019185.01.T01;Parent=MsG0480019185.01;Name=MsG0480019185.01.T01;_AED=0.35;_eAED=0.38;_QI=0|0.8|0.66|1|1|1|6|166|362
Chr4 exon 15602327 15602753 15602327 ID=MsG0480019185.01.T01:exon:5103;Parent=MsG0480019185.01.T01
Chr4 exon 15602911 15603021 15602911 ID=MsG0480019185.01.T01:exon:5102;Parent=MsG0480019185.01.T01
Chr4 exon 15603564 15603773 15603564 ID=MsG0480019185.01.T01:exon:5101;Parent=MsG0480019185.01.T01
Chr4 exon 15603957 15604090 15603957 ID=MsG0480019185.01.T01:exon:5100;Parent=MsG0480019185.01.T01
Chr4 exon 15604689 15604892 15604689 ID=MsG0480019185.01.T01:exon:5099;Parent=MsG0480019185.01.T01
Chr4 exon 15604996 15605164 15604996 ID=MsG0480019185.01.T01:exon:5098;Parent=MsG0480019185.01.T01
Chr4 CDS 15604996 15605164 15604996 ID=MsG0480019185.01.T01:cds;Parent=MsG0480019185.01.T01
Chr4 CDS 15604689 15604892 15604689 ID=MsG0480019185.01.T01:cds;Parent=MsG0480019185.01.T01
Chr4 CDS 15603957 15604090 15603957 ID=MsG0480019185.01.T01:cds;Parent=MsG0480019185.01.T01
Chr4 CDS 15603564 15603773 15603564 ID=MsG0480019185.01.T01:cds;Parent=MsG0480019185.01.T01
Chr4 CDS 15602911 15603021 15602911 ID=MsG0480019185.01.T01:cds;Parent=MsG0480019185.01.T01
Chr4 CDS 15602493 15602753 15602493 ID=MsG0480019185.01.T01:cds;Parent=MsG0480019185.01.T01
Chr4 three_prime_UTR 15602327 15602492 15602327 ID=MsG0480019185.01.T01:three_prime_utr;Parent=MsG0480019185.01.T01
Gene Sequence

>MsG0480019185.01.T01

CTGTTTTGTTATTTCATTACAGAAATGTCGACGATAGCACGTAGAGGCATCCATTACATGCAGAGACTGAATTCGGCAAATGTATCATCTGCTTTGCTAGAGAATGGCCAAAATCGTGTAATTGATGCTTCACTTACCCTGATTAGAGAAAGAGCAAAGCTTAAGGCAACGTTGTATGAAAGAAATGTGAATGTAGAAATTGCTTCTCTGCAGGGAGAACTTGTGCGTGCTTTGGGAGGTGCTGTTGCAACTTCATCACTTCTTGGAGTTCCTTTGGGTCATAATTCATCATTCCTTCAAGGGCCTGCATTTGCGCCTCCTCGCATTAGGGAAGCCATTTGGTGTGGTAGTACAAACTCGACAACCGAAGAAGGTAAGGATTTACAGGACGCACGAGTGCTAACTGATGTTGGTGATGTCCCTATTCAAGAAATTCGAGATTGTGGTGTAGATGATCATAGATTGATGAATGTCATTGGTGAATCTGTCAAGTTAGTGATGGAAGAGGATCCGTTAAGACCCTTAGTTTTGGGTGGTGATCACTCAATATCATTTCCCGTTATTAGAGCCGTCTCTGAGAAGCTTGGGGGACTGGTTGATGTTCTTCATCTTGATGCGCACCCTGATAACTATGATGAATTTGAAGGAAACTATTATTCACATGCTTCTTCCTTTGCTCGAGTCATGGAGGGTAACTATGTTCGGCGACTCTTGCAGGTTGGTATTCGTTCAATAACAACTGAAGGACGTGCGCAAGCAAAAAAGTTTGGGGTTGAGCAATATGAGATGCGAACATTTTCCAGAGACCGCCACTTCCTAGAGAACCTGAAACTAGGGGAAGGTGTGAAAGGTGTATATATCTCAATAGATGTGGATTGTCTTGATCCTGCTTTTGCTCCCGGAGTGTCTCACATAGAACCAGGAGGTCTTTCTTTCCGCGATGTTCTTAACATCCTACACAATCTTCAAGGTGATGTTGTTGCTGGAGATGTGGTTGAATTCAACCCACAACGCGATACTGTCGATGGAATGACTGCTATGGTAGCTGCTAAGTTGGTGAGAGAATTGGCTGCAAAGATTGCAAAATGA
Protein sequence

>MsG0480019185.01.T01

LFCYFITEMSTIARRGIHYMQRLNSANVSSALLENGQNRVIDASLTLIRERAKLKATLYERNVNVEIASLQGELVRALGGAVATSSLLGVPLGHNSSFLQGPAFAPPRIREAIWCGSTNSTTEEGKDLQDARVLTDVGDVPIQEIRDCGVDDHRLMNVIGESVKLVMEEDPLRPLVLGGDHSISFPVIRAVSEKLGGLVDVLHLDAHPDNYDEFEGNYYSHASSFARVMEGNYVRRLLQVGIRSITTEGRAQAKKFGVEQYEMRTFSRDRHFLENLKLGEGVKGVYISIDVDCLDPAFAPGVSHIEPGGLSFRDVLNILHNLQGDVVAGDVVEFNPQRDTVDGMTAMVAAKLVRELAAKIAK*