AlfalfaGEDB | MsG0480019447.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019447.01

KEGG: -
GO: GO:0006306 GO:0008168
PFAM: PF00145

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019447.01.T01	XP_003611920.2	79.397	398	21	3	322	660	164	559	0	636
MsG0480019447.01.T01	XP_003611920.2	75	228	26	4	5	232	96	292	2.92E-96	320
MsG0480019447.01.T01	XP_003611920.2	76.471	119	28	0	204	322	417	535	3.29E-45	182
MsG0480019447.01.T02	KEH27550.1	92.308	156	12	0	102	257	373	528	2.16E-94	297

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019447.01.T01	Q10SU5	56.74	319	88	5	385	659	255	567	3.41E-118	370
MsG0480019447.01.T02	Q10SU5	64.667	150	52	1	102	251	445	593	1.62E-60	204

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019447.01.T01	G7KC46	79.397	398	21	3	322	660	164	559	0.0	636
MsG0480019447.01.T02	A0A494G8U5	93.711	159	10	0	99	257	5	163	2.69e-106	314

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180003499.01	MsG0480019447.01	0.832829	7.562832e-56	8.551846e-53
MsG0480019447.01	MsG0780041746.01	0.820611	6.261275e-53	4.977591e-50
MsG0480019447.01	MsG0880046699.01	0.808978	2.374198e-50	1.376896e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019447.01.T01	MTR_5g019420	79.397	398	21	3	322	660	164	559	0.0	636
MsG0480019447.01.T01	MTR_5g019420	75.000	228	26	4	5	232	96	292	3.53e-100	320
MsG0480019447.01.T01	MTR_5g019420	76.471	119	28	0	204	322	417	535	3.98e-49	182
MsG0480019447.01.T01	MTR_3g011150	78.643	398	24	3	322	660	164	559	0.0	634
MsG0480019447.01.T01	MTR_3g011150	74.561	228	27	4	5	232	96	292	2.24e-99	318
MsG0480019447.01.T01	MTR_3g011150	73.950	119	31	0	204	322	417	535	1.29e-47	178
MsG0480019447.01.T01	MTR_5g019420	79.397	398	21	3	322	660	102	497	0.0	633
MsG0480019447.01.T01	MTR_5g019420	75.000	228	26	4	5	232	34	230	2.34e-100	319
MsG0480019447.01.T01	MTR_5g019420	76.471	119	28	0	204	322	355	473	2.64e-49	182
MsG0480019447.01.T01	MTR_6g005580	67.813	407	68	5	314	660	154	557	0.0	560

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019447.01.T01	AT5G14620	54.360	344	107	4	363	660	257	596	2.90e-117	366
MsG0480019447.01.T01	AT5G14620	50.420	119	59	0	204	322	454	572	6.27e-29	123
MsG0480019447.01.T01	AT5G14620	39.759	166	67	5	64	229	194	326	1.44e-24	109
MsG0480019447.01.T01	AT5G15380	48.831	385	136	8	325	659	218	591	4.06e-111	350
MsG0480019447.01.T01	AT5G15380	53.782	119	55	0	204	322	450	568	2.16e-31	130
MsG0480019447.01.T01	AT5G15380	37.209	172	71	6	62	230	188	325	1.10e-21	100
MsG0480019447.01.T01	AT3G17310	36.610	295	133	5	415	660	275	564	1.70e-54	197
MsG0480019447.01.T01	AT3G17310	47.664	107	56	0	226	332	444	550	2.82e-26	114
MsG0480019447.01.T01	AT3G17310	36.610	295	133	5	415	660	394	683	3.86e-54	199
MsG0480019447.01.T01	AT3G17310	47.664	107	56	0	226	332	563	669	4.80e-26	114

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Find 392 sgRNAs with CRISPR-Local

Find 1255 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAATTACAGCGCGGA+TGG	0.555445	4:+20540386	None:intergenic
AAACAATTCAGGTTTGTTCT+AGG	0.312748	4:+20534915	None:intergenic
AAACAATTCAGGTTTGTTCT+AGG	0.312748	4:+20542121	None:intergenic
AAACATGGGATTCAAGGAAG+AGG	0.585979	4:-20539528	MsG0480019447.01.T01:CDS
AAACCTGAATTGTTTGCAAA+TGG	0.411015	4:-20534907	MsG0480019447.01.T01:CDS
AAACTCCTCACCTGACCACC+TGG	0.540041	4:-20535564	MsG0480019447.01.T01:CDS
AAACTTACTACCTTATCTTC+TGG	0.376512	4:+20538902	None:intergenic
AAACTTACTACCTTATCTTC+TGG	0.376512	4:+20542688	None:intergenic
AAAGAATGTAGGTTTCTAAG+TGG	0.594379	4:-20540246	MsG0480019447.01.T01:five_prime_UTR
AAAGGGTGTAGGGTCAGAGG+TGG	0.627679	4:-20540211	MsG0480019447.01.T01:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATCAACAGTTTGCATCA+TGG	-	Chr4:20539913-20539932	MsG0480019447.01.T01:intron	30.0%
AAACACAAACAAAGGATTAG+TGG	+	Chr4:20546211-20546230	None:intergenic	30.0%
AAACCTGAATTGTTTGCAAA+CGG	-	Chr4:20529087-20529106	MsG0480019447.01.T01:intron	30.0%
AAACCTGAATTGTTTGCAAA+TGG	-	Chr4:20529087-20529106	MsG0480019447.01.T01:intron	30.0%
AAACTATTGGAAAGTTTCAC+AGG	+	Chr4:20534211-20534230	None:intergenic	30.0%
AAACTTACAACCTTATCTTC+TGG	+	Chr4:20535710-20535729	None:intergenic	30.0%
AAACTTACTACCTTATCTTC+TGG	+	Chr4:20528515-20528534	None:intergenic	30.0%
AAAGAAAATGGAAAAGGAAG+AGG	+	Chr4:20527563-20527582	None:intergenic	30.0%
AAAGAACAAAAGAGGTGAAA+TGG	+	Chr4:20527994-20528013	None:intergenic	30.0%
AAAGAATGTAGGTTTCTAAG+TGG	-	Chr4:20530954-20530973	MsG0480019447.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	20524772	20546450	20524772	ID=MsG0480019447.01;Name=MsG0480019447.01
Chr4	mRNA	20524772	20545249	20524772	ID=MsG0480019447.01.T01;Parent=MsG0480019447.01;Name=MsG0480019447.01.T01;_AED=0.31;_eAED=0.31;_QI=2364\|0.33\|0.43\|0.93\|0.53\|0.56\|16\|0\|735
Chr4	exon	20524772	20524917	20524772	ID=MsG0480019447.01.T01:exon:9951;Parent=MsG0480019447.01.T01
Chr4	exon	20530316	20530400	20530316	ID=MsG0480019447.01.T01:exon:9950;Parent=MsG0480019447.01.T01
Chr4	exon	20533472	20533807	20533472	ID=MsG0480019447.01.T01:exon:9949;Parent=MsG0480019447.01.T01
Chr4	exon	20534785	20535405	20534785	ID=MsG0480019447.01.T01:exon:9948;Parent=MsG0480019447.01.T01
Chr4	exon	20535538	20535590	20535538	ID=MsG0480019447.01.T01:exon:9947;Parent=MsG0480019447.01.T01
Chr4	exon	20536619	20536896	20536619	ID=MsG0480019447.01.T01:exon:9946;Parent=MsG0480019447.01.T01
Chr4	exon	20538533	20538816	20538533	ID=MsG0480019447.01.T01:exon:9945;Parent=MsG0480019447.01.T01
Chr4	exon	20538910	20539049	20538910	ID=MsG0480019447.01.T01:exon:9944;Parent=MsG0480019447.01.T01
Chr4	exon	20539501	20539600	20539501	ID=MsG0480019447.01.T01:exon:9943;Parent=MsG0480019447.01.T01
Chr4	exon	20539847	20539889	20539847	ID=MsG0480019447.01.T01:exon:9942;Parent=MsG0480019447.01.T01
Chr4	exon	20539977	20541271	20539977	ID=MsG0480019447.01.T01:exon:9941;Parent=MsG0480019447.01.T01
Chr4	exon	20541854	20542592	20541854	ID=MsG0480019447.01.T01:exon:9940;Parent=MsG0480019447.01.T01
Chr4	exon	20542699	20542790	20542699	ID=MsG0480019447.01.T01:exon:9939;Parent=MsG0480019447.01.T01
Chr4	exon	20542884	20543091	20542884	ID=MsG0480019447.01.T01:exon:9938;Parent=MsG0480019447.01.T01
Chr4	exon	20545145	20545249	20545145	ID=MsG0480019447.01.T01:exon:9936;Parent=MsG0480019447.01.T01
Chr4	five_prime_UTR	20545145	20545249	20545145	ID=MsG0480019447.01.T01:five_prime_utr;Parent=MsG0480019447.01.T01
Chr4	five_prime_UTR	20545010	20545056	20545010	ID=MsG0480019447.01.T01:five_prime_utr;Parent=MsG0480019447.01.T01
Chr4	five_prime_UTR	20542884	20543091	20542884	ID=MsG0480019447.01.T01:five_prime_utr;Parent=MsG0480019447.01.T01
Chr4	five_prime_UTR	20542699	20542790	20542699	ID=MsG0480019447.01.T01:five_prime_utr;Parent=MsG0480019447.01.T01
Chr4	five_prime_UTR	20541854	20542592	20541854	ID=MsG0480019447.01.T01:five_prime_utr;Parent=MsG0480019447.01.T01
Chr4	five_prime_UTR	20540099	20541271	20540099	ID=MsG0480019447.01.T01:five_prime_utr;Parent=MsG0480019447.01.T01
Chr4	CDS	20539977	20540098	20539977	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	CDS	20539847	20539889	20539847	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	CDS	20539501	20539600	20539501	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	CDS	20538910	20539049	20538910	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	CDS	20538533	20538816	20538533	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	CDS	20536619	20536896	20536619	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	CDS	20535538	20535590	20535538	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	CDS	20534785	20535405	20534785	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	CDS	20533472	20533807	20533472	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	CDS	20530316	20530400	20530316	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	CDS	20524772	20524917	20524772	ID=MsG0480019447.01.T01:cds;Parent=MsG0480019447.01.T01
Chr4	mRNA	20540837	20546450	20540837	ID=MsG0480019447.01.T02;Parent=MsG0480019447.01;Name=MsG0480019447.01.T02;_AED=0.51;_eAED=0.55;_QI=0\|0.25\|0.2\|0.8\|0\|0\|5\|0\|257
Chr4	exon	20546382	20546450	20546382	ID=MsG0480019447.01.T02:exon:9955;Parent=MsG0480019447.01.T02
Chr4	exon	20545758	20545875	20545758	ID=MsG0480019447.01.T02:exon:9954;Parent=MsG0480019447.01.T02
Chr4	exon	20543033	20543091	20543033	ID=MsG0480019447.01.T02:exon:9953;Parent=MsG0480019447.01.T02
Chr4	exon	20540837	20541317	20540837	ID=MsG0480019447.01.T02:exon:9952;Parent=MsG0480019447.01.T02
Chr4	CDS	20546382	20546450	20546382	ID=MsG0480019447.01.T02:cds;Parent=MsG0480019447.01.T02
Chr4	CDS	20545758	20545875	20545758	ID=MsG0480019447.01.T02:cds;Parent=MsG0480019447.01.T02
Chr4	CDS	20545010	20545056	20545010	ID=MsG0480019447.01.T02:cds;Parent=MsG0480019447.01.T02
Chr4	CDS	20543033	20543091	20543033	ID=MsG0480019447.01.T02:cds;Parent=MsG0480019447.01.T02
Chr4	CDS	20540837	20541317	20540837	ID=MsG0480019447.01.T02:cds;Parent=MsG0480019447.01.T02

Gene Sequence

>MsG0480019447.01.T02

ATGGATGTTCAACCATATGCCAAGCTTTATTATGTTTATGAGTTTGATATGATTGACTTTAGATCACCGGGTGATGATTCTAGTCTGGAGAGTGACAATTTTGATTGGAACACTGATGATGAACTTGAGATTGAGAGCTTTAATTCCTTAGTTCAACTGTTCCAAGCAGACAGACTATTACTGCTGCGCGAGGAAGATGAAGATAAACTACTTGAAGAGATTCTCACATATTCAGCTCTAGAAAGTTCTTCTCAGCAGCATCAGCAAGTTGAACCTGATCCCACCTCTTCAGATTTTCCATGTACAATTCGCTTTCCTACTGTGTTCAGTTTATTTCAAGTTGACACAATGGCGTATCACCTATCTGTTCTTAAAGAAATGTATCCTAATGGGATCAATCTTTTATCCCTCTTTTCTGGAATTAGTGGTGCAGAAGTAGCTCTCCATCGACTCAGCGTCCCTCTAAATAATGTTGTGTCTGTTGAAAAATCTGAAGTGAATAGGAACATTGTTAGAAGTTGGTGGGAACAAACTTATCAAAAAGGTAACTTGATTGATATCGATGATGTGCAACAGCTAGATGCTGAGCGTTTGGAGCAGCTCATGAGTGCATGTGGTGGTTTTGATTTCGTTGTTGGTGGGAGTCCATGCAATAACCTGGCTGGAAGCAACAGGGTTAGCCGGATTGGACTTGAGGGAACAGAGTCAGCCCTCTTTTACGATTACTTTCGAATTTTAGACTTGGTGAAAGCTATGGCGCCGAGGTTTCAATGA

>MsG0480019447.01.T01

ATGATTAAGAGGGAAAGTTCTTCTCAGCAGCATCAGCAAGTTGAACCAGATCCCACCTCTTCAGAGTATGCAGAGGGAGCTCATGGGATGATTTATCAGATGCGGTTTCTTTTTCTGATGAATGTCATGTACTCGGATTGTTTCTATTCTGCGACAGGGTATGCGGAAATGCCAAAATCTGTTTCTAGGAATGATGATAAACTGCTGTCTTTGGTAAACATGGGATTCAAGGAAGAGGAGGCTTTAATGGCCATAGAAAGATTAGATCAGACCATTTCATTGGACTCCCTGCTACAGCACAATACTGCAGGCCTAGACTCCTCACTTGACGGCCTGGTCGATTTTATCGGTGTTGCTCAACTGTTGAAAGAAGAAGATTCCCTTCTGCCTCCAGAAGATAAGGTACAACAATGCAGTGGGCATCCAAAACCAAGAAAGCGGAGTCTTTATGAATATGAAGTTCTAGGAAAGAAAAAACCGCAGGTGTCCGACAAGAGAACTCCATGTGAAGAGGAGGATGATGGCCAAACACTTAATCTGCCTCACCCAATGACGGGGTTTGGGGTTCCCAACGAGCCAAAGTCTATAATCACACACCGGACACTCCCTGAGAATGCTATTGGACCTCCCTACTTCTACTATGAGAATGTGGCACTAACACCGAAAGGTGTTTGGCAAATAATATCTAGTTTATTTCCGGTTGACACAGTGGCGTATCACCTATCTGTTCTTAATGAAATGTATCCTAATGGGATCAATCTTTTATCCCTCTTTTCTGGAATTGGTGGTGCAGAAGTAGCTCTCCATCGACTCGGTGTCCCTCTAAATAATGTTGTGTCGGTTGAAAAATCTGAAGTGAATAGGAACATTGTTAGAAGTTGGTGGGAACAAACTAATCAAAAAGGTAACTTGATTGATATCGATGATTTGCAACAGCTAGATGCTGAGCGTTTGGAGCAGCTCATGAGGCTAAACTCCTCACCTGACCACCTGGTCGATTTTATCGGTGTTGCTCAACTGCAACAATGCAGCGGGCATCCAAAACCAAGAAAGCGGAGTCTTTATGAATATGAAGTTCTAGGAAAGAAAAAACCAAAGGTGTCCGACAAGAGAACTCCATGTGAAGAGGAGGATGATGGCCAAACACTTAATCTGCCTAACCCAATGACGGGGTTTGGGGTTCCCAACGAGCCAAAGTCTATAATCACACACCGGACACTCCCTGAGAATGCTACTGGACCTCCCTACTTCTACTATGAGAATGTGGCACTAACACCGAAAGGTGTTTGGCAAAAAATATCTAGGTTCTTGTATGACGTGCAGCTGCAGCAGGAATACGTAGACTCAAAATATTTTTGTGCTGCGGCTAGGATAAGGGGATATGTTCACAATTTCCCTATTGCCAATAGATTTCCGCTTTTACCTCTTCCACCACGGACAATACTTGACGCATTCCCCCTATCGAGGAGATGGTGGCCATCATGGGATCCTAGAACAAACCTGAATTGTTTGCAAATGGTACACGGCAGTGCACAACTCACCGACAGAGTCAGAAAAAAGCTAGAAAGTTGTGAAGAATTTGAAGAACCATCGGAATCTGTCAAAAAGTATGTTTTAAAACAATGTCGGAAATGGAATTTGGTTGACACAGTGGCGTATCACCTATCTGTTCTTAAAGAAATGTATCCTAATGGGATCAATCTTTTATCCCTCTTTTCTGGAATTGGTGGTGCAGAAGTAGCTCTCCATCGACTCGGCGTCCCTTTAAATAATGTTGTGTCGGTTGAAAAATCTGAAGTGAATAGGAACATTGTTAGAAGTTGGTGGGAACAAACTAATCAAAAAGGTAACTTGATTGATATTGATGATGTGCAACAACTAGATGCTGAGCGTTTGGAGCAGCTCATGAGTGCATGTGGTGGTTTTGATCTCGTTGTTGGTGGGAGTCCATGCAATAACCTGGCTGGAAGCAACAGGATTCCTCTACAACCTTTGGGTTTCCTGGGGGCTATCTGTATTGCTATTAATCAAGCTATGACATCTGTTATCTTTGAATCAGATTCTTCTGGTGAAATGGCTTCTGATGAGGCAGCTTCTGATGATGCAGCTTCTGATGAGATGACTTCTGATGCAGCTTCTGATGATGCAGCTTCTGATGATGCAGCTTCTGATGATATACTTCAGAACTATTCTTCAGAAGCGACTTAA

Protein sequence

>MsG0480019447.01.T02

MDVQPYAKLYYVYEFDMIDFRSPGDDSSLESDNFDWNTDDELEIESFNSLVQLFQADRLLLLREEDEDKLLEEILTYSALESSSQQHQQVEPDPTSSDFPCTIRFPTVFSLFQVDTMAYHLSVLKEMYPNGINLLSLFSGISGAEVALHRLSVPLNNVVSVEKSEVNRNIVRSWWEQTYQKGNLIDIDDVQQLDAERLEQLMSACGGFDFVVGGSPCNNLAGSNRVSRIGLEGTESALFYDYFRILDLVKAMAPRFQ*

>MsG0480019447.01.T01

MIKRESSSQQHQQVEPDPTSSEYAEGAHGMIYQMRFLFLMNVMYSDCFYSATGYAEMPKSVSRNDDKLLSLVNMGFKEEEALMAIERLDQTISLDSLLQHNTAGLDSSLDGLVDFIGVAQLLKEEDSLLPPEDKVQQCSGHPKPRKRSLYEYEVLGKKKPQVSDKRTPCEEEDDGQTLNLPHPMTGFGVPNEPKSIITHRTLPENAIGPPYFYYENVALTPKGVWQIISSLFPVDTVAYHLSVLNEMYPNGINLLSLFSGIGGAEVALHRLGVPLNNVVSVEKSEVNRNIVRSWWEQTNQKGNLIDIDDLQQLDAERLEQLMRLNSSPDHLVDFIGVAQLQQCSGHPKPRKRSLYEYEVLGKKKPKVSDKRTPCEEEDDGQTLNLPNPMTGFGVPNEPKSIITHRTLPENATGPPYFYYENVALTPKGVWQKISRFLYDVQLQQEYVDSKYFCAAARIRGYVHNFPIANRFPLLPLPPRTILDAFPLSRRWWPSWDPRTNLNCLQMVHGSAQLTDRVRKKLESCEEFEEPSESVKKYVLKQCRKWNLVDTVAYHLSVLKEMYPNGINLLSLFSGIGGAEVALHRLGVPLNNVVSVEKSEVNRNIVRSWWEQTNQKGNLIDIDDVQQLDAERLEQLMSACGGFDLVVGGSPCNNLAGSNRIPLQPLGFLGAICIAINQAMTSVIFESDSSGEMASDEAASDDAASDEMTSDAASDDAASDDAASDDILQNYSSEAT*