Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019765.01.T01 | YP_009559975.1 | 84.615 | 65 | 8 | 1 | 1 | 63 | 1 | 65 | 5.62E-29 | 109 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019765.01.T01 | P51428 | 81.538 | 65 | 10 | 1 | 1 | 63 | 1 | 65 | 4.78E-30 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019765.01.T01 | A0A3S5HWW5 | 84.615 | 65 | 8 | 1 | 1 | 63 | 1 | 65 | 2.69e-29 | 109 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048011.01 | MsG0480019765.01 | 0.813847 | 2.080603e-51 | 1.373836e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019765.01.T01 | AT3G22300 | 59.016 | 61 | 25 | 0 | 3 | 63 | 128 | 188 | 3.20e-18 | 75.1 |
Find 6 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTATATAAGACTTGTGATTC+AGG | 0.342268 | 4:+26692904 | None:intergenic |
| TCTAATTTCAAATTGTTCTC+TGG | 0.353570 | 4:+26692849 | None:intergenic |
| CCGCTTTACACACGGAAGAT+TGG | 0.403918 | 4:-26692931 | MsG0480019765.01.T01:CDS |
| TCGAATCACTATGCGTATCT+TGG | 0.458963 | 4:+26692990 | None:intergenic |
| AATCACTATGCGTATCTTGG+TGG | 0.545623 | 4:+26692993 | None:intergenic |
| CCAATCTTCCGTGTGTAAAG+CGG | 0.567634 | 4:+26692931 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTAATTTCAAATTGTTCTC+TGG | + | Chr4:26692961-26692980 | None:intergenic | 25.0% |
| GTATATAAGACTTGTGATTC+AGG | + | Chr4:26692906-26692925 | None:intergenic | 30.0% | |
| ! | ATCTTTTGATCACCCATTTT+TGG | - | Chr4:26692839-26692858 | MsG0480019765.01.T01:CDS | 30.0% |
| ! | CTGGACTTTTTATCAATATG+AGG | + | Chr4:26692942-26692961 | None:intergenic | 30.0% |
| !! | ATCACCCATTTTTGGAAAAC+GGG | - | Chr4:26692847-26692866 | MsG0480019765.01.T01:CDS | 35.0% |
| !! | GATCACCCATTTTTGGAAAA+CGG | - | Chr4:26692846-26692865 | MsG0480019765.01.T01:CDS | 35.0% |
| AATCACTATGCGTATCTTGG+TGG | + | Chr4:26692817-26692836 | None:intergenic | 40.0% | |
| TCGAATCACTATGCGTATCT+TGG | + | Chr4:26692820-26692839 | None:intergenic | 40.0% | |
| !! | TCACCCATTTTTGGAAAACG+GGG | - | Chr4:26692848-26692867 | MsG0480019765.01.T01:CDS | 40.0% |
| CCAATCTTCCGTGTGTAAAG+CGG | + | Chr4:26692879-26692898 | None:intergenic | 45.0% | |
| !! | ACCCATTTTTGGAAAACGGG+GGG | - | Chr4:26692850-26692869 | MsG0480019765.01.T01:CDS | 45.0% |
| !! | CACCCATTTTTGGAAAACGG+GGG | - | Chr4:26692849-26692868 | MsG0480019765.01.T01:CDS | 45.0% |
| CCGCTTTACACACGGAAGAT+TGG | - | Chr4:26692876-26692895 | MsG0480019765.01.T01:CDS | 50.0% | |
| ! | AGCCCCCCGTTTTCCAAAAA+TGG | + | Chr4:26692855-26692874 | None:intergenic | 50.0% |
| ! | GCCCCCCGTTTTCCAAAAAT+GGG | + | Chr4:26692854-26692873 | None:intergenic | 50.0% |
| GGGGGCTTCCGCTTTACACA+CGG | - | Chr4:26692868-26692887 | MsG0480019765.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 26692810 | 26693019 | 26692810 | ID=MsG0480019765.01;Name=MsG0480019765.01 |
| Chr4 | mRNA | 26692810 | 26693019 | 26692810 | ID=MsG0480019765.01.T01;Parent=MsG0480019765.01;Name=MsG0480019765.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|69 |
| Chr4 | exon | 26692810 | 26693019 | 26692810 | ID=MsG0480019765.01.T01:exon:11680;Parent=MsG0480019765.01.T01 |
| Chr4 | CDS | 26692810 | 26693019 | 26692810 | ID=MsG0480019765.01.T01:cds;Parent=MsG0480019765.01.T01 |
| Gene Sequence |
| Protein sequence |
