Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019770.01.T01 | XP_013455462.2 | 69.318 | 88 | 12 | 2 | 5 | 92 | 3 | 75 | 1.63E-26 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019770.01.T01 | A0A072UK96 | 67.500 | 80 | 11 | 2 | 13 | 92 | 1 | 65 | 9.26e-22 | 90.9 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004147.01 | MsG0480019770.01 | 0.803200 | 3.904283e-49 | 1.950029e-46 |
| MsG0480019770.01 | MsG0780040488.01 | 0.806816 | 6.844380e-50 | 3.751244e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019770.01.T01 | MTR_4g037645 | 67.500 | 80 | 11 | 2 | 13 | 92 | 1 | 65 | 2.35e-25 | 90.9 |
| MsG0480019770.01.T01 | MTR_4g037615 | 67.500 | 80 | 11 | 2 | 13 | 92 | 1 | 65 | 3.29e-25 | 90.5 |
| MsG0480019770.01.T01 | MTR_4g037655 | 63.953 | 86 | 12 | 4 | 13 | 92 | 1 | 73 | 4.76e-24 | 87.0 |
| MsG0480019770.01.T01 | MTR_7g028465 | 47.727 | 88 | 25 | 3 | 13 | 92 | 1 | 75 | 1.05e-14 | 63.2 |
| MsG0480019770.01.T01 | MTR_4g036945 | 47.727 | 88 | 25 | 3 | 13 | 92 | 1 | 75 | 1.05e-14 | 63.2 |
| MsG0480019770.01.T01 | MTR_7g028468 | 46.154 | 91 | 24 | 4 | 13 | 92 | 1 | 77 | 1.96e-14 | 62.8 |
| MsG0480019770.01.T01 | MTR_4g036950 | 46.154 | 91 | 24 | 4 | 13 | 92 | 1 | 77 | 1.96e-14 | 62.8 |
| MsG0480019770.01.T01 | MTR_7g028462 | 42.222 | 90 | 26 | 3 | 13 | 92 | 1 | 74 | 2.67e-11 | 54.7 |
| MsG0480019770.01.T01 | MTR_4g036935 | 42.222 | 90 | 26 | 3 | 13 | 92 | 1 | 74 | 2.67e-11 | 54.7 |
| MsG0480019770.01.T01 | MTR_8g070055 | 45.455 | 66 | 23 | 1 | 13 | 78 | 1 | 53 | 4.89e-11 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGTGCTCTCTTATTT+TGG | 0.217478 | 4:+26757442 | MsG0480019770.01.T01:CDS |
| GTTGTTCTTGTATTAATATA+TGG | 0.309089 | 4:+26757625 | MsG0480019770.01.T01:intron |
| TGTATAGTTTCAGATGAAAT+AGG | 0.330215 | 4:+26757654 | MsG0480019770.01.T01:CDS |
| TTGTGGCAACGTGCCTCATC+AGG | 0.407929 | 4:-26757723 | None:intergenic |
| TATGGGGAAGTTCACTTACA+TGG | 0.454239 | 4:-26757542 | None:intergenic |
| TACAATGGACTCAATACATA+TGG | 0.479061 | 4:-26757402 | None:intergenic |
| GACTCCTTGCAGAAGAGTGT+GGG | 0.541799 | 4:+26757691 | MsG0480019770.01.T01:CDS |
| AGACTCCTTGCAGAAGAGTG+TGG | 0.554516 | 4:+26757690 | MsG0480019770.01.T01:CDS |
| TTTCATCAAAGGCATTAGCA+AGG | 0.590558 | 4:+26757492 | MsG0480019770.01.T01:CDS |
| CACTCCCACACTCTTCTGCA+AGG | 0.607244 | 4:-26757695 | None:intergenic |
| ATATCGTTCATGGAAAATTG+TGG | 0.617261 | 4:-26757740 | None:intergenic |
| TGGGAGTGATCATCCTGATG+AGG | 0.707936 | 4:+26757710 | MsG0480019770.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAACAAAATGGATATTAT+GGG | - | Chr4:26757562-26757581 | None:intergenic | 15.0% |
| !! | ATAACAAAATGGATATTATG+GGG | - | Chr4:26757561-26757580 | None:intergenic | 20.0% |
| !! | CAATAACAAAATGGATATTA+TGG | - | Chr4:26757563-26757582 | None:intergenic | 20.0% |
| !! | GTTGTTCTTGTATTAATATA+TGG | + | Chr4:26757625-26757644 | MsG0480019770.01.T01:intron | 20.0% |
| ! | GTGTAAATGCAATAACAAAA+TGG | - | Chr4:26757572-26757591 | None:intergenic | 25.0% |
| ! | TGTATAGTTTCAGATGAAAT+AGG | + | Chr4:26757654-26757673 | MsG0480019770.01.T01:CDS | 25.0% |
| ! | TACAATGGACTCAATACATA+TGG | - | Chr4:26757405-26757424 | None:intergenic | 30.0% |
| ! | TCTGTTGAGTTTTTCATCAA+AGG | + | Chr4:26757481-26757500 | MsG0480019770.01.T01:CDS | 30.0% |
| !!! | AAAGAAGTGCTCTCTTATTT+TGG | + | Chr4:26757442-26757461 | MsG0480019770.01.T01:CDS | 30.0% |
| GTCCATTGTAGCAAGAAAAA+TGG | + | Chr4:26757415-26757434 | MsG0480019770.01.T01:CDS | 35.0% | |
| !! | TTTCATCAAAGGCATTAGCA+AGG | + | Chr4:26757492-26757511 | MsG0480019770.01.T01:CDS | 35.0% |
| !!! | AGCCATTTTTCTTGCTACAA+TGG | - | Chr4:26757420-26757439 | None:intergenic | 35.0% |
| TATGGGGAAGTTCACTTACA+TGG | - | Chr4:26757545-26757564 | None:intergenic | 40.0% | |
| AGACTCCTTGCAGAAGAGTG+TGG | + | Chr4:26757690-26757709 | MsG0480019770.01.T01:CDS | 50.0% | |
| TGGGAGTGATCATCCTGATG+AGG | + | Chr4:26757710-26757729 | MsG0480019770.01.T01:CDS | 50.0% | |
| ! | GACTCCTTGCAGAAGAGTGT+GGG | + | Chr4:26757691-26757710 | MsG0480019770.01.T01:CDS | 50.0% |
| CACTCCCACACTCTTCTGCA+AGG | - | Chr4:26757698-26757717 | None:intergenic | 55.0% | |
| TTGTGGCAACGTGCCTCATC+AGG | - | Chr4:26757726-26757745 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 26757398 | 26757755 | 26757398 | ID=MsG0480019770.01;Name=MsG0480019770.01 |
| Chr4 | mRNA | 26757398 | 26757755 | 26757398 | ID=MsG0480019770.01.T01;Parent=MsG0480019770.01;Name=MsG0480019770.01.T01;_AED=0.36;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|93 |
| Chr4 | exon | 26757398 | 26757549 | 26757398 | ID=MsG0480019770.01.T01:exon:9196;Parent=MsG0480019770.01.T01 |
| Chr4 | exon | 26757626 | 26757755 | 26757626 | ID=MsG0480019770.01.T01:exon:9197;Parent=MsG0480019770.01.T01 |
| Chr4 | CDS | 26757398 | 26757549 | 26757398 | ID=MsG0480019770.01.T01:cds;Parent=MsG0480019770.01.T01 |
| Chr4 | CDS | 26757626 | 26757755 | 26757626 | ID=MsG0480019770.01.T01:cds;Parent=MsG0480019770.01.T01 |
| Gene Sequence |
| Protein sequence |
