AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019848.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019848.01.T01 MTR_0164s0070 72.254 173 9 1 9 142 37 209 6.51e-83 242
MsG0480019848.01.T01 MTR_2g101330 90.278 72 7 0 9 80 37 108 2.25e-39 135
MsG0480019848.01.T01 MTR_2g101330 86.667 45 6 0 80 124 231 275 7.72e-21 86.7
MsG0480019848.01.T01 MTR_8g062110 56.923 65 27 1 12 76 44 107 1.91e-19 82.0
MsG0480019848.01.T01 MTR_8g062110 56.923 65 27 1 12 76 44 107 1.91e-19 82.0
MsG0480019848.01.T01 MTR_8g062440 53.623 69 31 1 8 76 40 107 2.80e-19 82.0
MsG0480019848.01.T01 MTR_8g062110 56.923 65 27 1 12 76 44 107 2.86e-19 82.0
MsG0480019848.01.T01 MTR_8g062110 56.923 65 27 1 12 76 53 116 3.48e-19 82.0
MsG0480019848.01.T01 MTR_4g077100 52.778 72 33 1 12 83 46 116 5.79e-19 80.9
MsG0480019848.01.T01 MTR_8g062440 56.250 64 27 1 13 76 44 106 9.30e-19 80.9
MsG0480019848.01.T01 MTR_4g077100 55.385 65 28 1 12 76 46 109 1.02e-18 80.9
MsG0480019848.01.T01 MTR_8g062440 56.250 64 27 1 13 76 44 106 1.22e-18 80.5
MsG0480019848.01.T01 MTR_4g077130 53.846 65 29 1 12 76 44 107 1.01e-17 77.8
MsG0480019848.01.T01 MTR_3g005210 53.125 64 29 1 13 76 42 104 1.06e-17 77.8
MsG0480019848.01.T01 MTR_3g005170 53.125 64 29 1 13 76 46 108 6.70e-17 75.1
MsG0480019848.01.T01 MTR_3g005170 53.125 64 29 1 13 76 46 108 1.04e-16 75.1
MsG0480019848.01.T01 MTR_4g009040 48.529 68 35 0 13 80 48 115 1.37e-15 72.0
MsG0480019848.01.T01 MTR_4g009690 48.529 68 35 0 13 80 48 115 1.93e-15 71.6
MsG0480019848.01.T01 MTR_4g009690 48.529 68 35 0 13 80 48 115 2.02e-15 71.6
MsG0480019848.01.T01 MTR_1g022440 50.000 64 31 1 13 76 44 106 3.77e-14 68.2
MsG0480019848.01.T01 MTR_4g009040 45.833 72 35 1 13 80 48 119 1.26e-13 66.6
MsG0480019848.01.T01 MTR_1g022445 50.000 64 31 1 13 76 44 106 1.27e-13 66.6
MsG0480019848.01.T01 MTR_5g056600 44.595 74 41 0 12 85 39 112 3.79e-13 65.5
MsG0480019848.01.T01 MTR_2g104960 54.098 61 23 1 16 76 58 113 3.02e-12 62.8
MsG0480019848.01.T01 MTR_8g062710 44.828 58 32 0 13 70 45 102 9.63e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019848.01.T01 AT4G35420 67.647 68 21 1 9 76 40 106 1.34e-24 96.7
MsG0480019848.01.T01 AT1G09490 50.000 80 39 1 1 80 1 79 1.91e-18 79.7
MsG0480019848.01.T01 AT1G51410 48.913 92 42 2 12 103 44 130 2.25e-18 79.7
MsG0480019848.01.T01 AT1G66800 55.385 65 28 1 12 76 43 106 7.21e-18 78.6
MsG0480019848.01.T01 AT1G09490 52.857 70 32 1 11 80 42 110 1.75e-17 77.4
MsG0480019848.01.T01 AT5G19440 57.812 64 26 1 13 76 46 108 2.55e-17 77.0
MsG0480019848.01.T01 AT1G09500 48.571 70 36 0 11 80 42 111 1.26e-16 74.7
MsG0480019848.01.T01 AT1G09500 48.571 70 36 0 11 80 42 111 1.74e-16 74.7
MsG0480019848.01.T01 AT1G09500 48.571 70 36 0 11 80 8 77 1.80e-16 74.3
MsG0480019848.01.T01 AT1G09480 50.000 70 34 1 11 80 42 110 3.97e-16 73.6
MsG0480019848.01.T01 AT1G09510 51.515 66 31 1 11 76 42 106 4.31e-16 73.6
MsG0480019848.01.T01 AT1G09480 50.000 70 34 1 11 80 89 157 5.32e-16 73.6
MsG0480019848.01.T01 AT5G42800 47.059 68 35 1 9 76 40 106 8.49e-13 64.7
MsG0480019848.01.T01 AT2G33590 46.154 65 35 0 12 76 43 107 1.87e-12 63.5
MsG0480019848.01.T01 AT1G68540 36.957 92 55 1 11 102 38 126 2.11e-12 63.2
MsG0480019848.01.T01 AT5G58490 36.559 93 54 1 11 103 43 130 2.95e-12 62.8
MsG0480019848.01.T01 AT2G33600 43.077 65 37 0 12 76 43 107 9.61e-12 61.2
MsG0480019848.01.T01 AT2G02400 41.538 65 38 0 16 80 43 107 3.44e-11 59.7
MsG0480019848.01.T01 AT2G45400 42.667 75 40 2 3 76 11 83 3.80e-11 59.7
MsG0480019848.01.T01 AT1G15950 48.529 68 30 2 13 80 48 110 3.91e-11 59.3
MsG0480019848.01.T01 AT1G15950 48.529 68 30 2 13 80 48 110 3.95e-11 59.3
MsG0480019848.01.T01 AT1G15950 48.529 68 30 2 13 80 48 110 4.36e-11 59.7
MsG0480019848.01.T01 AT1G15950 48.529 68 30 2 13 80 48 110 4.36e-11 59.7
MsG0480019848.01.T01 AT1G15950 48.529 68 30 2 13 80 48 110 4.98e-11 59.3
MsG0480019848.01.T01 AT1G15950 48.529 68 30 2 13 80 48 110 5.06e-11 59.3

Find 33 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATAAAGTTGAACATTTA+TGG 0.085081 4:+28421112 MsG0480019848.01.T01:CDS
TAGCTTCGACAATGCGATTA+TGG 0.197247 4:+28421194 MsG0480019848.01.T01:CDS
ACTAGTCCAAGCTGATTTAA+TGG 0.254779 4:+28421164 MsG0480019848.01.T01:CDS
CGCAAGGACACTCTACCTTT+TGG 0.261628 4:-28422293 None:intergenic
CCTTTGCAGTTCAGCAATAA+TGG 0.265699 4:+28422217 MsG0480019848.01.T01:CDS
AGTTGAACATTTATGGAAGT+TGG 0.340616 4:+28421119 MsG0480019848.01.T01:CDS
AGGATCATTTGATATATGAT+TGG 0.357612 4:-28421253 None:intergenic
CAAAATACAGGCAGAAATCT+TGG 0.360481 4:+28421426 MsG0480019848.01.T01:intron
AGCTTCGACAATGCGATTAT+GGG 0.388369 4:+28421195 MsG0480019848.01.T01:CDS
AATAATGGATAATGATGATT+TGG 0.393430 4:+28422232 MsG0480019848.01.T01:CDS
GGATCATTTGATATATGATT+GGG 0.413478 4:-28421252 None:intergenic
TTTCTTCCATTAAATCAGCT+TGG 0.450929 4:-28421170 None:intergenic
TAGAGTGTCCTTGCGTTTCA+CGG 0.461971 4:+28422301 MsG0480019848.01.T01:CDS
TTTGATATATGATTGGGTAC+TGG 0.478192 4:-28421246 None:intergenic
ATGCTAGCAAATCGTTATCC+TGG 0.493557 4:+28422260 MsG0480019848.01.T01:CDS
TTTAGCGTACCTTGGACCGC+TGG 0.502028 4:-28421451 None:intergenic
AGGCTTTGTTCTCATAAAGA+AGG 0.503784 4:-28422181 None:intergenic
TCATATATCAAATGATCCTA+AGG 0.522536 4:+28421257 MsG0480019848.01.T01:CDS
CCTGGTTTCCCATTCCCAAA+AGG 0.524676 4:+28422278 MsG0480019848.01.T01:CDS
TGAACATTTATGGAAGTTGG+AGG 0.548932 4:+28421122 MsG0480019848.01.T01:CDS
CCATTATTGCTGAACTGCAA+AGG 0.550463 4:-28422217 None:intergenic
TATGAGAACAAAGCCTCTCA+TGG 0.568344 4:+28422188 MsG0480019848.01.T01:CDS
ATCTTGGAACCAGCGGTCCA+AGG 0.581499 4:+28421442 MsG0480019848.01.T01:CDS
ACTAGTTCGAGTCTTTCTGT+TGG 0.581772 4:-28421147 None:intergenic
AGGATAACGATTTGCTAGCA+TGG 0.597463 4:-28422258 None:intergenic
GAACATTTATGGAAGTTGGA+GGG 0.598986 4:+28421123 MsG0480019848.01.T01:CDS
AATGCGATTATGGGATGCAA+AGG 0.600616 4:+28421204 MsG0480019848.01.T01:CDS
GTGAACCACCGTGAAACGCA+AGG 0.619513 4:-28422309 None:intergenic
AGAACAAAGCCTCTCATGGT+AGG 0.634432 4:+28422192 MsG0480019848.01.T01:CDS
GCAAAGGTACCTACCATGAG+AGG 0.646700 4:-28422201 None:intergenic
TATATGATTGGGTACTGGAG+AGG 0.647630 4:-28421241 None:intergenic
GGCAGAAATCTTGGAACCAG+CGG 0.649586 4:+28421435 MsG0480019848.01.T01:intron
AGTGTCCTTGCGTTTCACGG+TGG 0.687838 4:+28422304 MsG0480019848.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCATTTATATTTTTCAT+AGG + Chr4:28421286-28421305 MsG0480019848.01.T01:intron 10.0%
!! AGTAATAGTAATACTATAAA+AGG - Chr4:28421721-28421740 None:intergenic 15.0%
!! GTAATAGTAATACTATAAAA+GGG - Chr4:28421720-28421739 None:intergenic 15.0%
!! TACAAATATATAATTACTTG+AGG - Chr4:28421638-28421657 None:intergenic 15.0%
!! ATATTGCTAATTAAACTAAC+AGG + Chr4:28422131-28422150 MsG0480019848.01.T01:intron 20.0%
!! TATTGCTAATTAAACTAACA+GGG + Chr4:28422132-28422151 MsG0480019848.01.T01:intron 20.0%
!!! AAGTCTAGCTTTTATAATAA+AGG - Chr4:28421866-28421885 None:intergenic 20.0%
!!! AATAATGGATAATGATGATT+TGG + Chr4:28422232-28422251 MsG0480019848.01.T01:CDS 20.0%
!!! TGTTAGTTTAATTAGCAATA+TGG - Chr4:28422132-28422151 None:intergenic 20.0%
! AGATAATGAAATGTAATTCG+AGG + Chr4:28421772-28421791 MsG0480019848.01.T01:intron 25.0%
! AGCATAAAGTTGAACATTTA+TGG + Chr4:28421112-28421131 MsG0480019848.01.T01:CDS 25.0%
! AGGATCATTTGATATATGAT+TGG - Chr4:28421256-28421275 None:intergenic 25.0%
! ATTCTACCATCATTCATAAT+TGG + Chr4:28421983-28422002 MsG0480019848.01.T01:intron 25.0%
! ATTGCTAATTAAACTAACAG+GGG + Chr4:28422133-28422152 MsG0480019848.01.T01:intron 25.0%
! CCTGCAAGTATATATAAAAA+TGG - Chr4:28421087-28421106 None:intergenic 25.0%
! GGATCATTTGATATATGATT+GGG - Chr4:28421255-28421274 None:intergenic 25.0%
! TAAATGAAATATCACACCTT+AGG - Chr4:28421276-28421295 None:intergenic 25.0%
! TCATATATCAAATGATCCTA+AGG + Chr4:28421257-28421276 MsG0480019848.01.T01:CDS 25.0%
!! AGACTTGTGTTATTGAATTA+GGG + Chr4:28421880-28421899 MsG0480019848.01.T01:intron 25.0%
!! TAGACTTGTGTTATTGAATT+AGG + Chr4:28421879-28421898 MsG0480019848.01.T01:intron 25.0%
!! TCAATTTTTGTCCAAAATAC+AGG + Chr4:28421414-28421433 MsG0480019848.01.T01:intron 25.0%
!!! CCATTTTTATATATACTTGC+AGG + Chr4:28421084-28421103 MsG0480019848.01.T01:CDS 25.0%
AGTTGAACATTTATGGAAGT+TGG + Chr4:28421119-28421138 MsG0480019848.01.T01:CDS 30.0%
CAATGAGAAAGGAATATACT+TGG + Chr4:28421955-28421974 MsG0480019848.01.T01:intron 30.0%
TAATCAAAATCCTCACATCA+TGG + Chr4:28421342-28421361 MsG0480019848.01.T01:intron 30.0%
TGATTAATGCATGAGCTTAT+AGG - Chr4:28421328-28421347 None:intergenic 30.0%
TTTCTTCCATTAAATCAGCT+TGG - Chr4:28421173-28421192 None:intergenic 30.0%
TTTGATATATGATTGGGTAC+TGG - Chr4:28421249-28421268 None:intergenic 30.0%
CAAAATACAGGCAGAAATCT+TGG + Chr4:28421426-28421445 MsG0480019848.01.T01:intron 35.0%
CATAAATATGTTGGCAAGCT+AGG - Chr4:28422010-28422029 None:intergenic 35.0%
CTAGGTCCAATTATGAATGA+TGG - Chr4:28421992-28422011 None:intergenic 35.0%
GAACATTTATGGAAGTTGGA+GGG + Chr4:28421123-28421142 MsG0480019848.01.T01:CDS 35.0%
GCAGTGGAACATAAATATGT+TGG - Chr4:28422019-28422038 None:intergenic 35.0%
TACATGCACACAAATGTTCT+AGG + Chr4:28422066-28422085 MsG0480019848.01.T01:intron 35.0%
TCGGAATATTGCAATGAGAA+AGG + Chr4:28421944-28421963 MsG0480019848.01.T01:intron 35.0%
TGAACATTTATGGAAGTTGG+AGG + Chr4:28421122-28421141 MsG0480019848.01.T01:CDS 35.0%
! ACTAGTCCAAGCTGATTTAA+TGG + Chr4:28421164-28421183 MsG0480019848.01.T01:CDS 35.0%
! CAAGATTTCTGCCTGTATTT+TGG - Chr4:28421428-28421447 None:intergenic 35.0%
! TGTGTTATTGAATTAGGGCA+TGG + Chr4:28421885-28421904 MsG0480019848.01.T01:intron 35.0%
!! AGGCTTTGTTCTCATAAAGA+AGG - Chr4:28422184-28422203 None:intergenic 35.0%
AATGCGATTATGGGATGCAA+AGG + Chr4:28421204-28421223 MsG0480019848.01.T01:CDS 40.0%
ACTAGTTCGAGTCTTTCTGT+TGG - Chr4:28421150-28421169 None:intergenic 40.0%
AGCTTCGACAATGCGATTAT+GGG + Chr4:28421195-28421214 MsG0480019848.01.T01:CDS 40.0%
ATGCTAGCAAATCGTTATCC+TGG + Chr4:28422260-28422279 MsG0480019848.01.T01:CDS 40.0%
CCATTATTGCTGAACTGCAA+AGG - Chr4:28422220-28422239 None:intergenic 40.0%
CCTTTGCAGTTCAGCAATAA+TGG + Chr4:28422217-28422236 MsG0480019848.01.T01:CDS 40.0%
GACTCGTCTATTGGTATGTT+TGG - Chr4:28421568-28421587 None:intergenic 40.0%
GCAACATATTTAGCGTACCT+TGG - Chr4:28421462-28421481 None:intergenic 40.0%
TACCAATAGACGAGTCATCT+TGG + Chr4:28421572-28421591 MsG0480019848.01.T01:intron 40.0%
TACTTGAGGAAATGTGTACC+TGG - Chr4:28421624-28421643 None:intergenic 40.0%
TAGCTTCGACAATGCGATTA+TGG + Chr4:28421194-28421213 MsG0480019848.01.T01:CDS 40.0%
TATGAGAACAAAGCCTCTCA+TGG + Chr4:28422188-28422207 MsG0480019848.01.T01:CDS 40.0%
TGTATAGTCTCCATGATGTG+AGG - Chr4:28421355-28421374 None:intergenic 40.0%
!! ACACTCTACCTTTTGGGAAT+GGG - Chr4:28422289-28422308 None:intergenic 40.0%
!! AGGATAACGATTTGCTAGCA+TGG - Chr4:28422261-28422280 None:intergenic 40.0%
!! TATATGATTGGGTACTGGAG+AGG - Chr4:28421244-28421263 None:intergenic 40.0%
ACTAACAGCTGAAGATGACG+CGG - Chr4:28421527-28421546 None:intergenic 45.0%
ACTGGCAGAAAAAGCAGCTT+CGG + Chr4:28421925-28421944 MsG0480019848.01.T01:intron 45.0%
AGAACAAAGCCTCTCATGGT+AGG + Chr4:28422192-28422211 MsG0480019848.01.T01:CDS 45.0%
CTCCAAGATGACTCGTCTAT+TGG - Chr4:28421577-28421596 None:intergenic 45.0%
GGAATTGTGTGAGAAACTCC+AGG + Chr4:28421603-28421622 MsG0480019848.01.T01:intron 45.0%
GGCTAACACAACTCGAACAA+GGG - Chr4:28421506-28421525 None:intergenic 45.0%
TTGAATTAGGGCATGGTACC+CGG + Chr4:28421892-28421911 MsG0480019848.01.T01:intron 45.0%
! GATGTGCTAGGCTTGTTCAA+AGG + Chr4:28422043-28422062 MsG0480019848.01.T01:intron 45.0%
! GCAAGGACACTCTACCTTTT+GGG - Chr4:28422295-28422314 None:intergenic 45.0%
! TAGAGTGTCCTTGCGTTTCA+CGG + Chr4:28422301-28422320 MsG0480019848.01.T01:CDS 45.0%
!! GACACTCTACCTTTTGGGAA+TGG - Chr4:28422290-28422309 None:intergenic 45.0%
!!! TTTTTTTTATATTTTACAAA+TGG + Chr4:28421812-28421831 MsG0480019848.01.T01:intron 5.0%
AACACAACTCGAACAAGGGC+TGG - Chr4:28421502-28421521 None:intergenic 50.0%
CCTGGTTTCCCATTCCCAAA+AGG + Chr4:28422278-28422297 MsG0480019848.01.T01:CDS 50.0%
CGAGTCATCTTGGAGCTCAT+AGG + Chr4:28421582-28421601 MsG0480019848.01.T01:intron 50.0%
CGGCTAACACAACTCGAACA+AGG - Chr4:28421507-28421526 None:intergenic 50.0%
GGCAGAAATCTTGGAACCAG+CGG + Chr4:28421435-28421454 MsG0480019848.01.T01:intron 50.0%
TCCACTGCATCTGATGTGCT+AGG + Chr4:28422031-28422050 MsG0480019848.01.T01:intron 50.0%
TCTGCCAGTGTCTTTGTGAC+CGG - Chr4:28421914-28421933 None:intergenic 50.0%
! CGCAAGGACACTCTACCTTT+TGG - Chr4:28422296-28422315 None:intergenic 50.0%
!! CCTTTTGGGAATGGGAAACC+AGG - Chr4:28422281-28422300 None:intergenic 50.0%
!! GCAAAGGTACCTACCATGAG+AGG - Chr4:28422204-28422223 None:intergenic 50.0%
AGTGTCCTTGCGTTTCACGG+TGG + Chr4:28422304-28422323 MsG0480019848.01.T01:CDS 55.0%
ATCTTGGAACCAGCGGTCCA+AGG + Chr4:28421442-28421461 MsG0480019848.01.T01:CDS 55.0%
CTGCCAGTGTCTTTGTGACC+GGG - Chr4:28421913-28421932 None:intergenic 55.0%
GCCTAGCACATCAGATGCAG+TGG - Chr4:28422035-28422054 None:intergenic 55.0%
GTACCCGGTCACAAAGACAC+TGG + Chr4:28421907-28421926 MsG0480019848.01.T01:intron 55.0%
GTGAACCACCGTGAAACGCA+AGG - Chr4:28422312-28422331 None:intergenic 55.0%
TTTAGCGTACCTTGGACCGC+TGG - Chr4:28421454-28421473 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 28421081 28422355 28421081 ID=MsG0480019848.01;Name=MsG0480019848.01
Chr4 mRNA 28421081 28422355 28421081 ID=MsG0480019848.01.T01;Parent=MsG0480019848.01;Name=MsG0480019848.01.T01;_AED=0.41;_eAED=0.41;_QI=0|0|0|1|0.5|0.33|3|0|142
Chr4 exon 28421081 28421278 28421081 ID=MsG0480019848.01.T01:exon:9344;Parent=MsG0480019848.01.T01
Chr4 exon 28421436 28421463 28421436 ID=MsG0480019848.01.T01:exon:9345;Parent=MsG0480019848.01.T01
Chr4 exon 28422153 28422355 28422153 ID=MsG0480019848.01.T01:exon:9346;Parent=MsG0480019848.01.T01
Chr4 CDS 28421081 28421278 28421081 ID=MsG0480019848.01.T01:cds;Parent=MsG0480019848.01.T01
Chr4 CDS 28421436 28421463 28421436 ID=MsG0480019848.01.T01:cds;Parent=MsG0480019848.01.T01
Chr4 CDS 28422153 28422355 28422153 ID=MsG0480019848.01.T01:cds;Parent=MsG0480019848.01.T01
Gene Sequence

>MsG0480019848.01.T01

ATGCCATTTTTATATATACTTGCAGGTAAGAAGCATAAAGTTGAACATTTATGGAAGTTGGAGGGACCAACAGAAAGACTCGAACTAGTCCAAGCTGATTTAATGGAAGAAAATAGCTTCGACAATGCGATTATGGGATGCAAAGGCGTCTTTCACATTGCCTCTCCAGTACCCAATCATATATCAAATGATCCTAAGGCAGAAATCTTGGAACCAGCGGTCCAAGGGGAAACATATGTTGCATTTAGTCACATCCTTCTTTATGAGAACAAAGCCTCTCATGGTAGGTACCTTTGCAGTTCAGCAATAATGGATAATGATGATTTGGTTGCCATGCTAGCAAATCGTTATCCTGGTTTCCCATTCCCAAAAGGTAGAGTGTCCTTGCGTTTCACGGTGGTTCACAATTCAGCTCTCCTAAAATTGTGA

Protein sequence

>MsG0480019848.01.T01

MPFLYILAGKKHKVEHLWKLEGPTERLELVQADLMEENSFDNAIMGCKGVFHIASPVPNHISNDPKAEILEPAVQGETYVAFSHILLYENKASHGRYLCSSAIMDNDDLVAMLANRYPGFPFPKGRVSLRFTVVHNSALLKL*