AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020494.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020494.01.T02 MTR_4g052390 98.291 351 5 1 1 351 1 350 0.0 709
MsG0480020494.01.T02 MTR_4g052380 76.068 351 63 2 1 351 1 330 0.0 543
MsG0480020494.01.T02 MTR_2g086600 25.325 308 188 7 44 337 21 300 3.68e-15 75.5
MsG0480020494.01.T02 MTR_2064s0010 37.634 93 58 0 111 203 53 145 3.90e-12 63.9
MsG0480020494.01.T02 MTR_3g104850 26.667 285 161 10 44 315 88 337 4.94e-12 66.6
MsG0480020494.01.T02 MTR_3g104850 26.224 286 169 9 40 315 34 287 5.57e-12 66.2
MsG0480020494.01.T01 MTR_4g052390 98.291 351 5 1 1 351 1 350 0.0 709
MsG0480020494.01.T01 MTR_4g052380 76.068 351 63 2 1 351 1 330 0.0 543
MsG0480020494.01.T01 MTR_2g086600 25.325 308 188 7 44 337 21 300 3.68e-15 75.5
MsG0480020494.01.T01 MTR_2064s0010 37.634 93 58 0 111 203 53 145 3.90e-12 63.9
MsG0480020494.01.T01 MTR_3g104850 26.667 285 161 10 44 315 88 337 4.94e-12 66.6
MsG0480020494.01.T01 MTR_3g104850 26.224 286 169 9 40 315 34 287 5.57e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020494.01.T02 AT5G22300 77.551 343 77 0 6 348 11 353 0.0 566
MsG0480020494.01.T02 AT3G44300 66.667 321 107 0 31 351 18 338 8.24e-166 466
MsG0480020494.01.T02 AT3G44320 61.823 351 122 1 1 351 7 345 1.00e-165 466
MsG0480020494.01.T02 AT3G44310 61.652 339 126 1 17 351 7 345 1.55e-159 451
MsG0480020494.01.T02 AT3G44310 61.652 339 126 1 17 351 7 345 1.55e-159 451
MsG0480020494.01.T02 AT5G22300 80.672 238 46 0 111 348 23 260 7.88e-146 412
MsG0480020494.01.T02 AT3G44310 65.471 223 77 0 129 351 1 223 1.00e-110 322
MsG0480020494.01.T02 AT3G44310 65.471 223 77 0 129 351 1 223 1.00e-110 322
MsG0480020494.01.T02 AT2G27450 25.667 300 181 7 44 329 22 293 2.22e-17 82.0
MsG0480020494.01.T02 AT2G27450 25.260 289 174 7 55 329 60 320 3.62e-15 75.9
MsG0480020494.01.T02 AT2G27450 25.439 228 142 5 116 329 1 214 1.61e-12 66.6
MsG0480020494.01.T01 AT5G22300 77.551 343 77 0 6 348 11 353 0.0 566
MsG0480020494.01.T01 AT3G44300 66.667 321 107 0 31 351 18 338 8.24e-166 466
MsG0480020494.01.T01 AT3G44320 61.823 351 122 1 1 351 7 345 1.00e-165 466
MsG0480020494.01.T01 AT3G44310 61.652 339 126 1 17 351 7 345 1.55e-159 451
MsG0480020494.01.T01 AT3G44310 61.652 339 126 1 17 351 7 345 1.55e-159 451
MsG0480020494.01.T01 AT5G22300 80.672 238 46 0 111 348 23 260 7.88e-146 412
MsG0480020494.01.T01 AT3G44310 65.471 223 77 0 129 351 1 223 1.00e-110 322
MsG0480020494.01.T01 AT3G44310 65.471 223 77 0 129 351 1 223 1.00e-110 322
MsG0480020494.01.T01 AT2G27450 25.667 300 181 7 44 329 22 293 2.22e-17 82.0
MsG0480020494.01.T01 AT2G27450 25.260 289 174 7 55 329 60 320 3.62e-15 75.9
MsG0480020494.01.T01 AT2G27450 25.439 228 142 5 116 329 1 214 1.61e-12 66.6

Find 20 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAATGACTTTGGATAAT+TGG 0.131815 4:+42429915 MsG0480020494.01.T01:three_prime_UTR
CAAGTAGTTTCTTCCTTTCA+TGG 0.273493 4:+42429817 MsG0480020494.01.T01:exon
ACAATCAAGCTTAGCACTTC+AGG 0.316148 4:-42429731 None:intergenic
CAGATCCATAACTGGAGGTT+TGG 0.349304 4:-42429850 None:intergenic
TGAACTTGTTAAAATGACTT+TGG 0.396092 4:+42429907 MsG0480020494.01.T01:three_prime_UTR
TTAATTTACAGATCCATAAC+TGG 0.425039 4:-42429858 None:intergenic
AAACTACTTGGTCTTCTCTT+CGG 0.431526 4:-42429804 None:intergenic
GACGCTGAGGCAAAAGTAAC+CGG 0.432233 4:-42429773 None:intergenic
GCAAAAGTAACCGGATTTGT+TGG 0.449893 4:-42429764 None:intergenic
AACTTCCAAACCTCCAGTTA+TGG 0.475738 4:+42429845 MsG0480020494.01.T01:three_prime_UTR
TAAATTAAGCATTTGCACAT+AGG 0.489443 4:+42429873 MsG0480020494.01.T01:three_prime_UTR
CATGAAAGGAAGAAACTACT+TGG 0.491348 4:-42429816 None:intergenic
TGGAAGTTGCTGACCATGAA+AGG 0.510864 4:-42429830 None:intergenic
CAAGGACCATCCAACAAATC+CGG 0.515395 4:+42429754 MsG0480020494.01.T01:CDS
AAGTAACCGGATTTGTTGGA+TGG 0.547807 4:-42429760 None:intergenic
ATATTGTAAGCTTTCACAGA+AGG 0.586024 4:+42430033 MsG0480020494.01.T01:three_prime_UTR
AATGCTCTTTGTAATCTGCA+TGG 0.590840 4:+42430000 MsG0480020494.01.T01:three_prime_UTR
ATTTACAGATCCATAACTGG+AGG 0.617669 4:-42429855 None:intergenic
AGTGCTAAGCTTGATTGTCA+AGG 0.633220 4:+42429736 MsG0480020494.01.T01:CDS
GCTTAATAAATATAGAAACA+AGG 0.651852 4:-42430074 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTACATTGATAAATTAA+TGG + Chr4:42428396-42428415 MsG0480020494.01.T01:intron 15.0%
!!! TATTGTTTCTTTTTTTAACA+AGG - Chr4:42430145-42430164 None:intergenic 15.0%
!!! TTTATGTTTGTTATCTTATT+AGG + Chr4:42428914-42428933 MsG0480020494.01.T01:intron 15.0%
!! AATAATTCCACTTAAACTAA+TGG + Chr4:42429429-42429448 MsG0480020494.01.T01:intron 20.0%
!! ATAATCGTTGTATATTACAA+GGG - Chr4:42426717-42426736 None:intergenic 20.0%
!! GCTTAATAAATATAGAAACA+AGG - Chr4:42430077-42430096 None:intergenic 20.0%
!! TTAAAATGACTTTGGATAAT+TGG + Chr4:42429915-42429934 MsG0480020494.01.T01:three_prime_UTR 20.0%
!!! AAGACTTAAATTTTGTAGTA+AGG + Chr4:42427238-42427257 MsG0480020494.01.T01:intron 20.0%
!!! TAAGCTATTTCTATAACAAA+CGG - Chr4:42428239-42428258 None:intergenic 20.0%
!!! TATAGAAACAAGTTGAAAAT+AGG - Chr4:42428179-42428198 None:intergenic 20.0%
!!! TTGATAAATTAATGGATCTT+TGG + Chr4:42428404-42428423 MsG0480020494.01.T01:intron 20.0%
!!! TTTATGATTTGTGAGTATTT+TGG + Chr4:42426866-42426885 MsG0480020494.01.T01:intron 20.0%
! AAACTATTTATGCGAACTTA+TGG + Chr4:42428130-42428149 MsG0480020494.01.T01:intron 25.0%
! CAACAAGGTTAGATAAAAAA+AGG - Chr4:42429284-42429303 None:intergenic 25.0%
! CATAATCGTTGTATATTACA+AGG - Chr4:42426718-42426737 None:intergenic 25.0%
! GAAACTCAAATTGGAAAAAT+AGG + Chr4:42427941-42427960 MsG0480020494.01.T01:CDS 25.0%
! GATAAAAAAAGGATGAAAAC+AGG - Chr4:42429273-42429292 None:intergenic 25.0%
! TAAAACACTGAAACAAAGAA+TGG - Chr4:42429371-42429390 None:intergenic 25.0%
! TAAATTAAGCATTTGCACAT+AGG + Chr4:42429873-42429892 MsG0480020494.01.T01:three_prime_UTR 25.0%
! TACAATATGTTGTCAAATAG+CGG + Chr4:42429039-42429058 MsG0480020494.01.T01:intron 25.0%
! TATAAAGTACATCTAGTGAT+GGG + Chr4:42427767-42427786 MsG0480020494.01.T01:CDS 25.0%
! TTAATTTACAGATCCATAAC+TGG - Chr4:42429861-42429880 None:intergenic 25.0%
!! AAATTTGTGTGAAGTATTTG+TGG + Chr4:42429342-42429361 MsG0480020494.01.T01:intron 25.0%
!!! CTTGTTTCTATAAACTCTTA+AGG + Chr4:42428187-42428206 MsG0480020494.01.T01:intron 25.0%
!!! CTTTTTTTATCTAACCTTGT+TGG + Chr4:42429282-42429301 MsG0480020494.01.T01:intron 25.0%
!!! GAGTATTTTGGTTTTAGATA+AGG + Chr4:42426878-42426897 MsG0480020494.01.T01:intron 25.0%
!!! TCAGTGTTTTAAAGTTTAGT+CGG + Chr4:42429380-42429399 MsG0480020494.01.T01:intron 25.0%
!!! TGAACTTGTTAAAATGACTT+TGG + Chr4:42429907-42429926 MsG0480020494.01.T01:three_prime_UTR 25.0%
!!! TGTTTGTTATCTTATTAGGA+TGG + Chr4:42428918-42428937 MsG0480020494.01.T01:intron 25.0%
AAATTCCTAATACTCATACC+AGG - Chr4:42428874-42428893 None:intergenic 30.0%
AAGTCGAAAACTAAGTGATA+AGG - Chr4:42427565-42427584 None:intergenic 30.0%
AATAATCCCAGAAACATAAC+AGG - Chr4:42427660-42427679 None:intergenic 30.0%
AATGTTGGTCATTCCAAATA+GGG - Chr4:42428067-42428086 None:intergenic 30.0%
ACCAACATTGTATAGTACTT+GGG + Chr4:42428078-42428097 MsG0480020494.01.T01:intron 30.0%
AGATGTAGAGATTAATGAGT+TGG + Chr4:42427341-42427360 MsG0480020494.01.T01:intron 30.0%
AGTACCTATTGTAGAAAAGA+TGG + Chr4:42427199-42427218 MsG0480020494.01.T01:intron 30.0%
AGTCGAAAACTAAGTGATAA+GGG - Chr4:42427564-42427583 None:intergenic 30.0%
ATATTGTAAGCTTTCACAGA+AGG + Chr4:42430033-42430052 MsG0480020494.01.T01:three_prime_UTR 30.0%
ATCGAATGACAAAAACTCAA+AGG - Chr4:42428040-42428059 None:intergenic 30.0%
ATGTTAAAAAGTGGCTATAG+TGG + Chr4:42428952-42428971 MsG0480020494.01.T01:intron 30.0%
ATTAAGATGTTCATCCAAAC+AGG + Chr4:42428310-42428329 MsG0480020494.01.T01:intron 30.0%
GACAACTCTTATATTTGACA+TGG + Chr4:42429158-42429177 MsG0480020494.01.T01:intron 30.0%
GGTATGAGTATTAGGAATTT+AGG + Chr4:42428874-42428893 MsG0480020494.01.T01:intron 30.0%
GTATAAAGTACATCTAGTGA+TGG + Chr4:42427766-42427785 MsG0480020494.01.T01:CDS 30.0%
TAAACAACCAAATCCAATAC+CGG - Chr4:42428382-42428401 None:intergenic 30.0%
TAAACTAATGGTTGAGATTG+AGG + Chr4:42429441-42429460 MsG0480020494.01.T01:intron 30.0%
TCTAACATAGTCATGCTTAT+AGG - Chr4:42429323-42429342 None:intergenic 30.0%
TGTAATACAGTTCAACCATT+AGG - Chr4:42428752-42428771 None:intergenic 30.0%
TTAAGATGTTCATCCAAACA+GGG + Chr4:42428311-42428330 MsG0480020494.01.T01:intron 30.0%
TTAGGACTAATAATGTCTCT+TGG + Chr4:42429531-42429550 MsG0480020494.01.T01:intron 30.0%
TTGAGAATCAAAGAAGAGAA+CGG - Chr4:42427828-42427847 None:intergenic 30.0%
! AGATCTGTGCAAAATTTTCA+AGG - Chr4:42429656-42429675 None:intergenic 30.0%
! ATCTCAACCATTAGTTTAAG+TGG - Chr4:42429439-42429458 None:intergenic 30.0%
! GTCTCTCTTATAGTTTTTCT+AGG - Chr4:42427312-42427331 None:intergenic 30.0%
! TACATTGCTGTTCTTAATAG+TGG - Chr4:42427995-42428014 None:intergenic 30.0%
! TAGTAGTTGTTGTTCTTGTT+AGG + Chr4:42427610-42427629 MsG0480020494.01.T01:intron 30.0%
! TCCAATTTGAGTTTCAAACA+CGG - Chr4:42427936-42427955 None:intergenic 30.0%
! TGAAAATTTTGCACAGATCT+TGG + Chr4:42429656-42429675 MsG0480020494.01.T01:intron 30.0%
! TGCTACCTTTTTATTGTTGT+AGG + Chr4:42427707-42427726 MsG0480020494.01.T01:intron 30.0%
! TGGTTGATATTTACACCTAA+TGG + Chr4:42428734-42428753 MsG0480020494.01.T01:intron 30.0%
!! AAGTTTGATTTTGATGTGGT+TGG + Chr4:42429695-42429714 MsG0480020494.01.T01:CDS 30.0%
!! TCACTTAGTTTTCGACTTTT+TGG + Chr4:42427567-42427586 MsG0480020494.01.T01:intron 30.0%
!! TGCAAAATTTTCAAGGATCA+GGG - Chr4:42429649-42429668 None:intergenic 30.0%
!! TTTAGTTTGAGCTTTCTAGA+AGG + Chr4:42426820-42426839 MsG0480020494.01.T01:intron 30.0%
!! TTTCTAGAAGGCTGAATTTT+CGG + Chr4:42426832-42426851 MsG0480020494.01.T01:intron 30.0%
!! TTTCTGGGATTATTTACTAG+TGG + Chr4:42427666-42427685 MsG0480020494.01.T01:intron 30.0%
!!! AATGGATCTTTGGTTTTAAC+AGG + Chr4:42428414-42428433 MsG0480020494.01.T01:intron 30.0%
AACACTGAAACAAAGAATGG+AGG - Chr4:42429368-42429387 None:intergenic 35.0%
AATGCTCTTTGTAATCTGCA+TGG + Chr4:42430000-42430019 MsG0480020494.01.T01:three_prime_UTR 35.0%
ACAGGTATAGGAACTACTAA+AGG - Chr4:42427642-42427661 None:intergenic 35.0%
ACCCAAGTACTATACAATGT+TGG - Chr4:42428082-42428101 None:intergenic 35.0%
ACCTATTGTAGAAAAGATGG+TGG + Chr4:42427202-42427221 MsG0480020494.01.T01:intron 35.0%
ACTTTGTGGGAATAAAGACT+TGG + Chr4:42427428-42427447 MsG0480020494.01.T01:intron 35.0%
AGAACAGCAATGTATGCTAA+AGG + Chr4:42428001-42428020 MsG0480020494.01.T01:CDS 35.0%
AGTCTTTATTCCCACAAAGT+AGG - Chr4:42427428-42427447 None:intergenic 35.0%
ATACAACAGAATCAGGTGTA+AGG - Chr4:42428608-42428627 None:intergenic 35.0%
ATGCAATCTAATGCTACTGA+GGG - Chr4:42428356-42428375 None:intergenic 35.0%
ATTTACAGATCCATAACTGG+AGG - Chr4:42429858-42429877 None:intergenic 35.0%
CAAGTAGTTTCTTCCTTTCA+TGG + Chr4:42429817-42429836 MsG0480020494.01.T01:exon 35.0%
CAATGTTGGTCATTCCAAAT+AGG - Chr4:42428068-42428087 None:intergenic 35.0%
CAGGACCTACAACAATAAAA+AGG - Chr4:42427715-42427734 None:intergenic 35.0%
CATGAAAGGAAGAAACTACT+TGG - Chr4:42429819-42429838 None:intergenic 35.0%
CCTATACCTGTTATGTTTCT+GGG + Chr4:42427651-42427670 MsG0480020494.01.T01:intron 35.0%
CCTTTGATAAATTCTTGAGC+CGG - Chr4:42429478-42429497 None:intergenic 35.0%
CTGTGTTTATCAAACGCTTT+AGG + Chr4:42429513-42429532 MsG0480020494.01.T01:intron 35.0%
GAACCTTCAAAAACATAGTC+TGG - Chr4:42428576-42428595 None:intergenic 35.0%
GACCAACATTGTATAGTACT+TGG + Chr4:42428077-42428096 MsG0480020494.01.T01:intron 35.0%
GAGTAGCATTCAACTCATAA+TGG - Chr4:42429627-42429646 None:intergenic 35.0%
GCTCAAGAATTTATCAAAGG+TGG + Chr4:42429478-42429497 MsG0480020494.01.T01:intron 35.0%
GGAACAGCTATGTTAAAAAG+TGG + Chr4:42428943-42428962 MsG0480020494.01.T01:intron 35.0%
GTCTTTATTCCCACAAAGTA+GGG - Chr4:42427427-42427446 None:intergenic 35.0%
GTTCTCTTCTTTGATTCTCA+AGG + Chr4:42427827-42427846 MsG0480020494.01.T01:CDS 35.0%
TAACCACCAATAAATGCTTC+TGG - Chr4:42426957-42426976 None:intergenic 35.0%
TAGCATTAGATTGCATATGC+CGG + Chr4:42428360-42428379 MsG0480020494.01.T01:intron 35.0%
TATGCAATCTAATGCTACTG+AGG - Chr4:42428357-42428376 None:intergenic 35.0%
TCATTCGATTACACCCTATT+TGG + Chr4:42428051-42428070 MsG0480020494.01.T01:intron 35.0%
TCCTATACCTGTTATGTTTC+TGG + Chr4:42427650-42427669 MsG0480020494.01.T01:intron 35.0%
TGTCATTATATCACCATCAG+GGG + Chr4:42428641-42428660 MsG0480020494.01.T01:CDS 35.0%
TGTTATCTTATTAGGATGGC+TGG + Chr4:42428922-42428941 MsG0480020494.01.T01:intron 35.0%
TGTTGAAATTGTAGCCAACA+AGG - Chr4:42429299-42429318 None:intergenic 35.0%
TTCTTACGACAGTGAAAGTA+TGG - Chr4:42429600-42429619 None:intergenic 35.0%
! AAACTACTTGGTCTTCTCTT+CGG - Chr4:42429807-42429826 None:intergenic 35.0%
! AGTGTCATTATATCACCATC+AGG + Chr4:42428639-42428658 MsG0480020494.01.T01:CDS 35.0%
! CCAATTTGAGTTTCAAACAC+GGG - Chr4:42427935-42427954 None:intergenic 35.0%
! GTGTCATTATATCACCATCA+GGG + Chr4:42428640-42428659 MsG0480020494.01.T01:CDS 35.0%
! TCCACCATCTTTTCTACAAT+AGG - Chr4:42427206-42427225 None:intergenic 35.0%
! TTTTATTGTTGTAGGTCCTG+AGG + Chr4:42427715-42427734 MsG0480020494.01.T01:intron 35.0%
!! AGCGAAGTTTGATTTTGATG+TGG + Chr4:42429691-42429710 MsG0480020494.01.T01:CDS 35.0%
!! GTGCAAAATTTTCAAGGATC+AGG - Chr4:42429650-42429669 None:intergenic 35.0%
!! GTGTTTCCAGAAGCATTTAT+TGG + Chr4:42426948-42426967 MsG0480020494.01.T01:CDS 35.0%
!! TAGAAAAGATGGTGGAAACT+TGG + Chr4:42427210-42427229 MsG0480020494.01.T01:intron 35.0%
!! TTTCCAGAAGCATTTATTGG+TGG + Chr4:42426951-42426970 MsG0480020494.01.T01:CDS 35.0%
!!! AGGTGGATGTTTTGTTTTGT+CGG + Chr4:42428512-42428531 MsG0480020494.01.T01:CDS 35.0%
!!! ATCTTTGGTTTTAACAGGTG+TGG + Chr4:42428419-42428438 MsG0480020494.01.T01:intron 35.0%
!!! CATCTTGTTTTCCCAACAAA+TGG - Chr4:42427972-42427991 None:intergenic 35.0%
!!! CTGAATTTTCGGTGTTGAAT+TGG + Chr4:42426843-42426862 MsG0480020494.01.T01:intron 35.0%
AACGCATTATCTGGGGATTT+GGG + Chr4:42427894-42427913 MsG0480020494.01.T01:CDS 40.0%
AACTTCCAAACCTCCAGTTA+TGG + Chr4:42429845-42429864 MsG0480020494.01.T01:three_prime_UTR 40.0%
AAGATGTTGTGTTGGATGTG+TGG + Chr4:42427175-42427194 MsG0480020494.01.T01:intron 40.0%
AAGCTTGAATGACAGTTGCT+CGG - Chr4:42426648-42426667 None:intergenic 40.0%
ACAATCAAGCTTAGCACTTC+AGG - Chr4:42429734-42429753 None:intergenic 40.0%
AGTAAGGAGACTAGATGAGA+TGG + Chr4:42427254-42427273 MsG0480020494.01.T01:intron 40.0%
AGTGCTAAGCTTGATTGTCA+AGG + Chr4:42429736-42429755 MsG0480020494.01.T01:CDS 40.0%
ATATCCAAAAAGGCCCTGTT+TGG - Chr4:42428327-42428346 None:intergenic 40.0%
ATCCACCTTCAAGTGCAATA+TGG - Chr4:42428500-42428519 None:intergenic 40.0%
ATGACCCATATTGCACTTGA+AGG + Chr4:42428492-42428511 MsG0480020494.01.T01:CDS 40.0%
ATGTGTACCTGACACATTTC+TGG + Chr4:42428820-42428839 MsG0480020494.01.T01:intron 40.0%
CACAACAACTTTGATTGGAC+AGG - Chr4:42427501-42427520 None:intergenic 40.0%
CAGAGATGAAGATGTTGTGT+TGG + Chr4:42427167-42427186 MsG0480020494.01.T01:intron 40.0%
CATACTAATGTAAGTGCACC+CGG - Chr4:42429139-42429158 None:intergenic 40.0%
CATGAATGACAGACATACCT+AGG - Chr4:42428716-42428735 None:intergenic 40.0%
CCCAGAAACATAACAGGTAT+AGG - Chr4:42427654-42427673 None:intergenic 40.0%
CCGGCTCAAGAATTTATCAA+AGG + Chr4:42429475-42429494 MsG0480020494.01.T01:intron 40.0%
CTAAGGGTAGAGAAGATTTC+AGG + Chr4:42427021-42427040 MsG0480020494.01.T01:CDS 40.0%
CTAGGTATGTCTGTCATTCA+TGG + Chr4:42428714-42428733 MsG0480020494.01.T01:intron 40.0%
CTTCGAATTAAGCTCTTACC+AGG - Chr4:42427074-42427093 None:intergenic 40.0%
GATTCTCAAGGTCATTACCT+AGG + Chr4:42427839-42427858 MsG0480020494.01.T01:CDS 40.0%
GATTGAGGTTGTTTGATGTC+CGG + Chr4:42429456-42429475 MsG0480020494.01.T01:intron 40.0%
GCAAAAGTAACCGGATTTGT+TGG - Chr4:42429767-42429786 None:intergenic 40.0%
GTGCACACAACAACTTTGAT+TGG - Chr4:42427506-42427525 None:intergenic 40.0%
TAGATTGCATATGCCGGTAT+TGG + Chr4:42428366-42428385 MsG0480020494.01.T01:intron 40.0%
TAGTGTACCAGAAATGTGTC+AGG - Chr4:42428830-42428849 None:intergenic 40.0%
TCCACCTTCAAGTGCAATAT+GGG - Chr4:42428499-42428518 None:intergenic 40.0%
TGACTTCCCAAGAGAAAACA+TGG + Chr4:42426534-42426553 MsG0480020494.01.T01:intron 40.0%
TGCAATCTAATGCTACTGAG+GGG - Chr4:42428355-42428374 None:intergenic 40.0%
TTCGAAGAATCAGCAAGCAT+TGG + Chr4:42427087-42427106 MsG0480020494.01.T01:intron 40.0%
TTGAGTTTCAAACACGGGAA+TGG - Chr4:42427930-42427949 None:intergenic 40.0%
! AAATAGGTGCTGCCATTTGT+TGG + Chr4:42427957-42427976 MsG0480020494.01.T01:CDS 40.0%
! AATAGGTGCTGCCATTTGTT+GGG + Chr4:42427958-42427977 MsG0480020494.01.T01:CDS 40.0%
! ATTGGTAACCGTACTGCTAA+GGG + Chr4:42427005-42427024 MsG0480020494.01.T01:CDS 40.0%
! CCCGTGTTTGAAACTCAAAT+TGG + Chr4:42427932-42427951 MsG0480020494.01.T01:CDS 40.0%
! TCAAAAACATAGTCTGGAGC+AGG - Chr4:42428570-42428589 None:intergenic 40.0%
! TGATTTTCCTGTGCTTTCCT+AGG - Chr4:42427859-42427878 None:intergenic 40.0%
! TGTCTCTTGGTCTTTCTTGA+AGG + Chr4:42429544-42429563 MsG0480020494.01.T01:intron 40.0%
! TTCTGGAAACACAACAAGCT+GGG - Chr4:42426940-42426959 None:intergenic 40.0%
!! AAGTAACCGGATTTGTTGGA+TGG - Chr4:42429763-42429782 None:intergenic 40.0%
!! AATTCTCTGGAATCAGCAGT+TGG - Chr4:42428459-42428478 None:intergenic 40.0%
!! GACAGTGAGAGCATTGAAAT+CGG - Chr4:42426626-42426645 None:intergenic 40.0%
!! GCTCCAGACTATGTTTTTGA+AGG + Chr4:42428570-42428589 MsG0480020494.01.T01:CDS 40.0%
!!! TCTGGTTTTGGTGTTTCCAT+TGG + Chr4:42426987-42427006 MsG0480020494.01.T01:CDS 40.0%
!!! TGGTTTTAGATAAGGCTGAG+AGG + Chr4:42426886-42426905 MsG0480020494.01.T01:intron 40.0%
AAACCAGTTCTGTAGGAGGA+AGG + Chr4:42428536-42428555 MsG0480020494.01.T01:CDS 45.0%
AAGGAGACTAGATGAGATGG+AGG + Chr4:42427257-42427276 MsG0480020494.01.T01:intron 45.0%
AAGTGAGAGACCTAAAGTGG+CGG - Chr4:42426692-42426711 None:intergenic 45.0%
AATCCACGATTGACAACACC+GGG + Chr4:42429118-42429137 MsG0480020494.01.T01:intron 45.0%
AATCCTTCCTCCTACAGAAC+TGG - Chr4:42428542-42428561 None:intergenic 45.0%
ACAAAGTAGGGTCAGCTACA+TGG - Chr4:42427415-42427434 None:intergenic 45.0%
ACAACCACTCCAACCATCAA+CGG + Chr4:42426568-42426587 MsG0480020494.01.T01:CDS 45.0%
ACCCATATTGCACTTGAAGG+TGG + Chr4:42428495-42428514 MsG0480020494.01.T01:CDS 45.0%
ACGCATTATCTGGGGATTTG+GGG + Chr4:42427895-42427914 MsG0480020494.01.T01:CDS 45.0%
AGATAAGGCTGAGAGGTTGT+TGG + Chr4:42426893-42426912 MsG0480020494.01.T01:intron 45.0%
ATAATGCGTTCCAGTGCTGT+TGG - Chr4:42427884-42427903 None:intergenic 45.0%
ATGTAGCTGACCCTACTTTG+TGG + Chr4:42427414-42427433 MsG0480020494.01.T01:intron 45.0%
ATTATCTGGGGATTTGGGGA+TGG + Chr4:42427899-42427918 MsG0480020494.01.T01:CDS 45.0%
CAAGGACCATCCAACAAATC+CGG + Chr4:42429754-42429773 MsG0480020494.01.T01:CDS 45.0%
CAATCCACGATTGACAACAC+CGG + Chr4:42429117-42429136 MsG0480020494.01.T01:intron 45.0%
CAATCTAATGCTACTGAGGG+GGG - Chr4:42428353-42428372 None:intergenic 45.0%
CAGATCCATAACTGGAGGTT+TGG - Chr4:42429853-42429872 None:intergenic 45.0%
CCAGCACATACAACAGAATC+AGG - Chr4:42428615-42428634 None:intergenic 45.0%
CCTGATTCTGTTGTATGTGC+TGG + Chr4:42428612-42428631 MsG0480020494.01.T01:CDS 45.0%
CTTAGCAGTACGGTTACCAA+TGG - Chr4:42427006-42427025 None:intergenic 45.0%
CTTCTCTACCCTTAGCAGTA+CGG - Chr4:42427016-42427035 None:intergenic 45.0%
CTTCTTCTGCCTCTAGTGAT+TGG - Chr4:42427288-42427307 None:intergenic 45.0%
GAAAATCATGCCAACAGCAC+TGG + Chr4:42427871-42427890 MsG0480020494.01.T01:CDS 45.0%
GAACGCATTATCTGGGGATT+TGG + Chr4:42427893-42427912 MsG0480020494.01.T01:CDS 45.0%
GAGAAGTGAGAGACCTAAAG+TGG - Chr4:42426695-42426714 None:intergenic 45.0%
GATTCTGTTGTATGTGCTGG+AGG + Chr4:42428615-42428634 MsG0480020494.01.T01:CDS 45.0%
GCAATCTAATGCTACTGAGG+GGG - Chr4:42428354-42428373 None:intergenic 45.0%
GTACACTATGCCAGACTACT+GGG + Chr4:42428842-42428861 MsG0480020494.01.T01:intron 45.0%
TATTGTTGTAGGTCCTGAGG+TGG + Chr4:42427718-42427737 MsG0480020494.01.T01:intron 45.0%
TGATGCTTGCCACAATTCTC+TGG - Chr4:42428472-42428491 None:intergenic 45.0%
TGGAAGTTGCTGACCATGAA+AGG - Chr4:42429833-42429852 None:intergenic 45.0%
TGGTGCCTGGTATGAGTATT+AGG + Chr4:42428866-42428885 MsG0480020494.01.T01:intron 45.0%
TGTAGCTGACCCTACTTTGT+GGG + Chr4:42427415-42427434 MsG0480020494.01.T01:intron 45.0%
TTCTGCCTCTAGTGATTGGA+TGG - Chr4:42427284-42427303 None:intergenic 45.0%
TTGGCTGGACCCAATTATGA+GGG + Chr4:42428669-42428688 MsG0480020494.01.T01:CDS 45.0%
TTTGGCTGGACCCAATTATG+AGG + Chr4:42428668-42428687 MsG0480020494.01.T01:CDS 45.0%
! AAAACATAGTCTGGAGCAGG+TGG - Chr4:42428567-42428586 None:intergenic 45.0%
! ACGAGTGCCATGTTTTCTCT+TGG - Chr4:42426544-42426563 None:intergenic 45.0%
! AGCACTGGAACGCATTATCT+GGG + Chr4:42427886-42427905 MsG0480020494.01.T01:CDS 45.0%
! AGGGGGGAGTATATCCAAAA+AGG - Chr4:42428337-42428356 None:intergenic 45.0%
! CATTGGTAACCGTACTGCTA+AGG + Chr4:42427004-42427023 MsG0480020494.01.T01:CDS 45.0%
! CGAGTGCCATGTTTTCTCTT+GGG - Chr4:42426543-42426562 None:intergenic 45.0%
! CTTCTGGAAACACAACAAGC+TGG - Chr4:42426941-42426960 None:intergenic 45.0%
! GCATATGCCGGTATTGGATT+TGG + Chr4:42428372-42428391 MsG0480020494.01.T01:intron 45.0%
! GTCATTACCTAGGAAAGCAC+AGG + Chr4:42427849-42427868 MsG0480020494.01.T01:CDS 45.0%
! TCATCCAAACAGGGCCTTTT+TGG + Chr4:42428320-42428339 MsG0480020494.01.T01:intron 45.0%
! TGTTGTCAATCGTGGATTGC+AGG - Chr4:42429116-42429135 None:intergenic 45.0%
! TTCGGTTTTTGTTGACGCTG+AGG - Chr4:42429789-42429808 None:intergenic 45.0%
! TTTATTGGTGGTTATCCGCG+TGG + Chr4:42426963-42426982 MsG0480020494.01.T01:CDS 45.0%
!! AGTGATGGGTGTGATAGAGA+GGG + Chr4:42427781-42427800 MsG0480020494.01.T01:CDS 45.0%
!! CTGCTGATTCCAGAGAATTG+TGG + Chr4:42428460-42428479 MsG0480020494.01.T01:CDS 45.0%
!! TAGTGATGGGTGTGATAGAG+AGG + Chr4:42427780-42427799 MsG0480020494.01.T01:CDS 45.0%
ACACCAAAACCAGAACCACG+CGG - Chr4:42426981-42427000 None:intergenic 50.0%
AGGTCCTGAGGTGGATAGAT+TGG + Chr4:42427727-42427746 MsG0480020494.01.T01:intron 50.0%
AGTGAGAGACCTAAAGTGGC+GGG - Chr4:42426691-42426710 None:intergenic 50.0%
ATGGGTGTGATAGAGAGGGA+TGG + Chr4:42427785-42427804 MsG0480020494.01.T01:CDS 50.0%
GACGCTGAGGCAAAAGTAAC+CGG - Chr4:42429776-42429795 None:intergenic 50.0%
GAGGACCATCCAATCACTAG+AGG + Chr4:42427276-42427295 MsG0480020494.01.T01:intron 50.0%
GATAGATTGGCAGCAATGGC+AGG + Chr4:42427740-42427759 MsG0480020494.01.T01:CDS 50.0%
GCACTGGAACGCATTATCTG+GGG + Chr4:42427887-42427906 MsG0480020494.01.T01:CDS 50.0%
GCGCTAATATCAGCAGACCT+AGG + Chr4:42428696-42428715 MsG0480020494.01.T01:CDS 50.0%
GGTACACTATGCCAGACTAC+TGG + Chr4:42428841-42428860 MsG0480020494.01.T01:intron 50.0%
GGTGGATAGATTGGCAGCAA+TGG + Chr4:42427736-42427755 MsG0480020494.01.T01:CDS 50.0%
TCAGCTGCTATTGATGTGCC+TGG + Chr4:42427053-42427072 MsG0480020494.01.T01:CDS 50.0%
TGGCTGGACCCAATTATGAG+GGG + Chr4:42428670-42428689 MsG0480020494.01.T01:CDS 50.0%
TGTCGGCAAACCAGTTCTGT+AGG + Chr4:42428529-42428548 MsG0480020494.01.T01:CDS 50.0%
TTGGCTGAAGCTGCTAGCTA+TGG + Chr4:42426912-42426931 MsG0480020494.01.T01:CDS 50.0%
! ATCACCATCAGGGGCTGTTT+TGG + Chr4:42428650-42428669 MsG0480020494.01.T01:CDS 50.0%
! CAGCACTGGAACGCATTATC+TGG + Chr4:42427885-42427904 MsG0480020494.01.T01:CDS 50.0%
!! ATCGGAACCCATGTCGACTT+CGG - Chr4:42426608-42426627 None:intergenic 50.0%
!!! TATCCGCGTGGTTCTGGTTT+TGG + Chr4:42426975-42426994 MsG0480020494.01.T01:CDS 50.0%
ACTATGCCAGACTACTGGGC+TGG + Chr4:42428846-42428865 MsG0480020494.01.T01:intron 55.0%
ACTCATCGCCGAAGTCGACA+TGG + Chr4:42426597-42426616 MsG0480020494.01.T01:CDS 55.0%
AGCGCCTCCCCCTCATAATT+GGG - Chr4:42428681-42428700 None:intergenic 55.0%
CGGCAAACCAGTTCTGTAGG+AGG + Chr4:42428532-42428551 MsG0480020494.01.T01:CDS 55.0%
CTCATCGCCGAAGTCGACAT+GGG + Chr4:42426598-42426617 MsG0480020494.01.T01:CDS 55.0%
GATGAGTGGTTCGCCGTTGA+TGG - Chr4:42426584-42426603 None:intergenic 55.0%
GCTGCCAATCTATCCACCTC+AGG - Chr4:42427734-42427753 None:intergenic 55.0%
GGCTGGACCCAATTATGAGG+GGG + Chr4:42428671-42428690 MsG0480020494.01.T01:CDS 55.0%
TAGCGCCTCCCCCTCATAAT+TGG - Chr4:42428682-42428701 None:intergenic 55.0%
TGGACCCAATTATGAGGGGG+AGG + Chr4:42428674-42428693 MsG0480020494.01.T01:CDS 55.0%
! TACGACACTCCCGCCACTTT+AGG + Chr4:42426679-42426698 MsG0480020494.01.T01:CDS 55.0%
!! AGTGGTTCGCCGTTGATGGT+TGG - Chr4:42426580-42426599 None:intergenic 55.0%
!! ATGTCGACTTCGGCGATGAG+TGG - Chr4:42426598-42426617 None:intergenic 55.0%
!! GGGAGTGTCGTAGAAGATGG+TGG - Chr4:42426671-42426690 None:intergenic 55.0%
!! TTCGCCGTTGATGGTTGGAG+TGG - Chr4:42426575-42426594 None:intergenic 55.0%
CCAGCCAAAACAGCCCCTGA+TGG - Chr4:42428657-42428676 None:intergenic 60.0%
GGTGGTTATCCGCGTGGTTC+TGG + Chr4:42426969-42426988 MsG0480020494.01.T01:CDS 60.0%
!! GCACCCGGTGTTGTCAATCG+TGG - Chr4:42429124-42429143 None:intergenic 60.0%
!! GGCGGGAGTGTCGTAGAAGA+TGG - Chr4:42426674-42426693 None:intergenic 60.0%
!!! CCATCAGGGGCTGTTTTGGC+TGG + Chr4:42428654-42428673 MsG0480020494.01.T01:CDS 60.0%
CAGGCACCAGCCCAGTAGTC+TGG - Chr4:42428855-42428874 None:intergenic 65.0%
!! CAGACTACTGGGCTGGTGCC+TGG + Chr4:42428853-42428872 MsG0480020494.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 42426499 42430190 42426499 ID=MsG0480020494.01;Name=MsG0480020494.01
Chr4 mRNA 42426499 42430109 42426499 ID=MsG0480020494.01.T01;Parent=MsG0480020494.01;Name=MsG0480020494.01.T01;_AED=0.32;_eAED=0.32;_QI=54|1|1|1|0.8|0.66|6|262|351
Chr4 exon 42429672 42430054 42429672 ID=MsG0480020494.01.T01:exon:15440;Parent=MsG0480020494.01.T01
Chr4 exon 42430079 42430109 42430079 ID=MsG0480020494.01.T01:exon:15441;Parent=MsG0480020494.01.T01
Chr4 five_prime_UTR 42426499 42426552 42426499 ID=MsG0480020494.01.T01:five_prime_utr;Parent=MsG0480020494.01.T01
Chr4 CDS 42426553 42426700 42426553 ID=MsG0480020494.01.T01:cds;Parent=MsG0480020494.01.T01
Chr4 CDS 42426895 42427074 42426895 ID=MsG0480020494.01.T01:cds;Parent=MsG0480020494.01.T01
Chr4 CDS 42427729 42428022 42427729 ID=MsG0480020494.01.T01:cds;Parent=MsG0480020494.01.T01
Chr4 CDS 42428436 42428717 42428436 ID=MsG0480020494.01.T01:cds;Parent=MsG0480020494.01.T01
Chr4 CDS 42429672 42429823 42429672 ID=MsG0480020494.01.T01:cds;Parent=MsG0480020494.01.T01
Chr4 three_prime_UTR 42429824 42430054 42429824 ID=MsG0480020494.01.T01:three_prime_utr;Parent=MsG0480020494.01.T01
Chr4 three_prime_UTR 42430079 42430109 42430079 ID=MsG0480020494.01.T01:three_prime_utr;Parent=MsG0480020494.01.T01
Chr4 mRNA 42426499 42430190 42426499 ID=MsG0480020494.01.T02;Parent=MsG0480020494.01;Name=MsG0480020494.01.T02;_AED=0.30;_eAED=0.30;_QI=54|1|1|1|1|1|5|367|351
Chr4 exon 42429672 42430190 42429672 ID=MsG0480020494.01.T02:exon:15442;Parent=MsG0480020494.01.T02
Chr4 five_prime_UTR 42426499 42426552 42426499 ID=MsG0480020494.01.T02:five_prime_utr;Parent=MsG0480020494.01.T02
Chr4 CDS 42426553 42426700 42426553 ID=MsG0480020494.01.T02:cds;Parent=MsG0480020494.01.T02
Chr4 CDS 42426895 42427074 42426895 ID=MsG0480020494.01.T02:cds;Parent=MsG0480020494.01.T02
Chr4 CDS 42427729 42428022 42427729 ID=MsG0480020494.01.T02:cds;Parent=MsG0480020494.01.T02
Chr4 CDS 42428436 42428717 42428436 ID=MsG0480020494.01.T02:cds;Parent=MsG0480020494.01.T02
Chr4 CDS 42429672 42429823 42429672 ID=MsG0480020494.01.T02:cds;Parent=MsG0480020494.01.T02
Chr4 three_prime_UTR 42429824 42430190 42429824 ID=MsG0480020494.01.T02:three_prime_utr;Parent=MsG0480020494.01.T02
Gene Sequence

>MsG0480020494.01.T02

ATGGCACTCGTAACAACAACCACTCCAACCATCAACGGCGAACCACTCATCGCCGAAGTCGACATGGGTTCCGATTTCAATGCTCTCACTGTCCGAGCAACTGTCATTCAAGCTTCCACCATCTTCTACGACACTCCCGCCACTTTAGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTAGCTATGGTTCCCAGCTTGTTGTGTTTCCAGAAGCATTTATTGGTGGTTATCCGCGTGGTTCTGGTTTTGGTGTTTCCATTGGTAACCGTACTGCTAAGGGTAGAGAAGATTTCAGGAAGTATCATTCAGCTGCTATTGATGTGCCTGGTCCTGAGGTGGATAGATTGGCAGCAATGGCAGGAAAGTATAAAGTACATCTAGTGATGGGTGTGATAGAGAGGGATGGCTATACACTCTACTGCACCGTTCTCTTCTTTGATTCTCAAGGTCATTACCTAGGAAAGCACAGGAAAATCATGCCAACAGCACTGGAACGCATTATCTGGGGATTTGGGGATGGATCAACCATTCCCGTGTTTGAAACTCAAATTGGAAAAATAGGTGCTGCCATTTGTTGGGAAAACAAGATGCCACTATTAAGAACAGCAATGTATGCTAAAGGTGTGGAAATATATTGTGCACCAACTGCTGATTCCAGAGAATTGTGGCAAGCATCAATGACCCATATTGCACTTGAAGGTGGATGTTTTGTTTTGTCGGCAAACCAGTTCTGTAGGAGGAAGGATTATCCACCTGCTCCAGACTATGTTTTTGAAGGTTCAGAAGAGAACCTTACACCTGATTCTGTTGTATGTGCTGGAGGTAGTGTCATTATATCACCATCAGGGGCTGTTTTGGCTGGACCCAATTATGAGGGGGAGGCGCTAATATCAGCAGACCTAGATCTTGGCGAAATAGCAAGAGCGAAGTTTGATTTTGATGTGGTTGGACACTATGCAAGACCTGAAGTGCTAAGCTTGATTGTCAAGGACCATCCAACAAATCCGGTTACTTTTGCCTCAGCGTCAACAAAAACCGAAGAGAAGACCAAGTAG

>MsG0480020494.01.T01

ATGGCACTCGTAACAACAACCACTCCAACCATCAACGGCGAACCACTCATCGCCGAAGTCGACATGGGTTCCGATTTCAATGCTCTCACTGTCCGAGCAACTGTCATTCAAGCTTCCACCATCTTCTACGACACTCCCGCCACTTTAGATAAGGCTGAGAGGTTGTTGGCTGAAGCTGCTAGCTATGGTTCCCAGCTTGTTGTGTTTCCAGAAGCATTTATTGGTGGTTATCCGCGTGGTTCTGGTTTTGGTGTTTCCATTGGTAACCGTACTGCTAAGGGTAGAGAAGATTTCAGGAAGTATCATTCAGCTGCTATTGATGTGCCTGGTCCTGAGGTGGATAGATTGGCAGCAATGGCAGGAAAGTATAAAGTACATCTAGTGATGGGTGTGATAGAGAGGGATGGCTATACACTCTACTGCACCGTTCTCTTCTTTGATTCTCAAGGTCATTACCTAGGAAAGCACAGGAAAATCATGCCAACAGCACTGGAACGCATTATCTGGGGATTTGGGGATGGATCAACCATTCCCGTGTTTGAAACTCAAATTGGAAAAATAGGTGCTGCCATTTGTTGGGAAAACAAGATGCCACTATTAAGAACAGCAATGTATGCTAAAGGTGTGGAAATATATTGTGCACCAACTGCTGATTCCAGAGAATTGTGGCAAGCATCAATGACCCATATTGCACTTGAAGGTGGATGTTTTGTTTTGTCGGCAAACCAGTTCTGTAGGAGGAAGGATTATCCACCTGCTCCAGACTATGTTTTTGAAGGTTCAGAAGAGAACCTTACACCTGATTCTGTTGTATGTGCTGGAGGTAGTGTCATTATATCACCATCAGGGGCTGTTTTGGCTGGACCCAATTATGAGGGGGAGGCGCTAATATCAGCAGACCTAGATCTTGGCGAAATAGCAAGAGCGAAGTTTGATTTTGATGTGGTTGGACACTATGCAAGACCTGAAGTGCTAAGCTTGATTGTCAAGGACCATCCAACAAATCCGGTTACTTTTGCCTCAGCGTCAACAAAAACCGAAGAGAAGACCAAGTAG
Protein sequence

>MsG0480020494.01.T02

MALVTTTTPTINGEPLIAEVDMGSDFNALTVRATVIQASTIFYDTPATLDKAERLLAEAASYGSQLVVFPEAFIGGYPRGSGFGVSIGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKVHLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVFETQIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSRELWQASMTHIALEGGCFVLSANQFCRRKDYPPAPDYVFEGSEENLTPDSVVCAGGSVIISPSGAVLAGPNYEGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLIVKDHPTNPVTFASASTKTEEKTK*

>MsG0480020494.01.T01

MALVTTTTPTINGEPLIAEVDMGSDFNALTVRATVIQASTIFYDTPATLDKAERLLAEAASYGSQLVVFPEAFIGGYPRGSGFGVSIGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKVHLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVFETQIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSRELWQASMTHIALEGGCFVLSANQFCRRKDYPPAPDYVFEGSEENLTPDSVVCAGGSVIISPSGAVLAGPNYEGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLIVKDHPTNPVTFASASTKTEEKTK*