AlfalfaGEDB | MsG0480021121.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021121.01

KEGG: K09284
GO: GO:0003677 GO:0003700 GO:0006355
PFAM: PF00847

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021121.01.T01	XP_016577819.1	72.222	144	10	2	237	380	152	265	1.30E-59	210
MsG0480021121.01.T01	XP_016577819.1	71.56	109	11	1	68	176	160	248	6.31E-41	160

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021121.01.T01	B8AXC3	71.528	144	11	2	237	380	189	302	2.97E-50	179

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021121.01.T01	A0A2G2ZDT0	72.222	144	10	2	237	380	152	265	6.23e-60	210
MsG0480021121.01.T01	A0A3Q7H3G3	71.034	145	21	2	237	380	170	294	4.87e-57	211

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480021121.01	MsG0880044075.01	0.801362	9.330995e-49	4.447754e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021121.01.T01	MTR_2g093060	78.472	144	1	2	237	380	152	265	5.11e-58	196
MsG0480021121.01.T01	MTR_2g093060	79.817	109	2	1	68	176	160	248	1.33e-39	147
MsG0480021121.01.T01	MTR_2g093060	78.472	144	1	2	237	380	152	265	5.34e-58	196
MsG0480021121.01.T01	MTR_2g093060	79.817	109	2	1	68	176	160	248	1.37e-39	147
MsG0480021121.01.T01	MTR_4g061200	70.139	144	13	2	237	380	149	262	1.11e-49	172
MsG0480021121.01.T01	MTR_4g061200	68.807	109	14	1	68	176	157	245	2.11e-31	123
MsG0480021121.01.T01	MTR_4g061200	70.139	144	13	2	237	380	149	262	1.53e-49	173
MsG0480021121.01.T01	MTR_4g061200	68.807	109	14	1	68	176	157	245	2.89e-31	124
MsG0480021121.01.T01	MTR_7g100590	68.056	144	16	2	237	380	157	270	1.51e-48	171
MsG0480021121.01.T01	MTR_7g100590	67.890	109	15	1	68	176	165	253	1.46e-31	125

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021121.01.T01	AT2G28550	72.414	145	10	2	237	381	171	285	3.13e-51	174
MsG0480021121.01.T01	AT2G28550	71.560	109	11	1	68	176	179	267	4.20e-33	126
MsG0480021121.01.T01	AT2G28550	72.222	144	10	2	237	380	171	284	3.61e-50	175
MsG0480021121.01.T01	AT2G28550	71.560	109	11	1	68	176	179	267	3.28e-32	126
MsG0480021121.01.T01	AT4G36920	65.972	144	19	2	237	380	149	262	1.59e-47	167
MsG0480021121.01.T01	AT4G36920	65.138	109	18	1	68	176	157	245	6.05e-30	119
MsG0480021121.01.T01	AT4G36920	65.972	144	19	2	237	380	149	262	1.59e-47	167
MsG0480021121.01.T01	AT4G36920	65.138	109	18	1	68	176	157	245	6.05e-30	119
MsG0480021121.01.T01	AT5G67180	62.937	143	23	2	237	379	114	226	6.87e-45	155
MsG0480021121.01.T01	AT5G67180	61.468	109	22	1	68	176	122	210	7.75e-28	110

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Find 84 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTGTATTTGGGCTTGT+AGG	0.380016	4:-53803089	MsG0480021121.01.T01:CDS
AAACTTGTATTTGGGCTTGT+AGG	0.380016	4:-53808398	MsG0480021121.01.T01:CDS
ACACGGCTCATGCTGCTGCT+AGG	0.548016	4:-53803058	MsG0480021121.01.T01:intron
ACACGGCTCATGCTGCTGCT+AGG	0.548016	4:-53808367	MsG0480021121.01.T01:intron
ACAGATGAAGAATCTTTCAA+AGG	0.598696	4:-53802622	MsG0480021121.01.T01:CDS
ACAGATGAAGAATCTTTCAA+AGG	0.598696	4:-53807931	MsG0480021121.01.T01:CDS
ACAGCGAAGTAGAAGCTACA+AGG	0.619068	4:-53807471	MsG0480021121.01.T01:intron
ACAGCGAAGTAGAAGCTGCA+AGG	0.657584	4:-53802162	MsG0480021121.01.T01:CDS
ACAGTAACGGTTTCTCAAGG+GGG	0.706952	4:-53802572	MsG0480021121.01.T01:CDS
ACAGTAACGGTTTCTCAAGG+GGG	0.706952	4:-53807881	MsG0480021121.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACATACATTATACAAGGCA+AGG	+	Chr4:53802648-53802667	None:intergenic	30.0%
AACTTTCACCAGAAAAACAA+AGG	+	Chr4:53807226-53807245	None:intergenic	30.0%
AATCATGGTACTTCAAAACA+AGG	-	Chr4:53803002-53803021	MsG0480021121.01.T01:intron	30.0%
AATGAATCCAAATACGATTG+TGG	+	Chr4:53802573-53802592	None:intergenic	30.0%
AATTAGCTACGAAATAGCTA+CGG	-	Chr4:53804876-53804895	MsG0480021121.01.T01:intron	30.0%
ACAAGGAACAAGAAAACTAT+GGG	+	Chr4:53802521-53802540	None:intergenic	30.0%
ACAGATGAAGAATCTTTCAA+AGG	-	Chr4:53803916-53803935	MsG0480021121.01.T01:intron	30.0%
ACTTTCAGCTTATTCTCAAA+TGG	-	Chr4:53804213-53804232	MsG0480021121.01.T01:intron	30.0%
ATAAGAACAGAGCAACATAT+TGG	+	Chr4:53804484-53804503	None:intergenic	30.0%
ATATACTAAACTATGGGACA+AGG	+	Chr4:53802538-53802557	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	53802153	53809716	53802153	ID=MsG0480021121.01;Name=MsG0480021121.01
Chr4	mRNA	53802153	53809716	53802153	ID=MsG0480021121.01.T01;Parent=MsG0480021121.01;Name=MsG0480021121.01.T01;_AED=0.41;_eAED=0.45;_QI=0\|0\|0\|0.61\|0.66\|0.53\|13\|0\|381
Chr4	exon	53809641	53809716	53809641	ID=MsG0480021121.01.T01:exon:8005;Parent=MsG0480021121.01.T01
Chr4	exon	53809111	53809175	53809111	ID=MsG0480021121.01.T01:exon:8004;Parent=MsG0480021121.01.T01
Chr4	exon	53808368	53808462	53808368	ID=MsG0480021121.01.T01:exon:8003;Parent=MsG0480021121.01.T01
Chr4	exon	53808172	53808263	53808172	ID=MsG0480021121.01.T01:exon:8002;Parent=MsG0480021121.01.T01
Chr4	exon	53807804	53808005	53807804	ID=MsG0480021121.01.T01:exon:8001;Parent=MsG0480021121.01.T01
Chr4	exon	53807633	53807705	53807633	ID=MsG0480021121.01.T01:exon:8000;Parent=MsG0480021121.01.T01
Chr4	exon	53807472	53807516	53807472	ID=MsG0480021121.01.T01:exon:7999;Parent=MsG0480021121.01.T01
Chr4	exon	53805537	53805598	53805537	ID=MsG0480021121.01.T01:exon:7998;Parent=MsG0480021121.01.T01
Chr4	exon	53803199	53803224	53803199	ID=MsG0480021121.01.T01:exon:7997;Parent=MsG0480021121.01.T01
Chr4	exon	53803059	53803119	53803059	ID=MsG0480021121.01.T01:exon:7996;Parent=MsG0480021121.01.T01
Chr4	exon	53802863	53802954	53802863	ID=MsG0480021121.01.T01:exon:7995;Parent=MsG0480021121.01.T01
Chr4	exon	53802495	53802696	53802495	ID=MsG0480021121.01.T01:exon:7994;Parent=MsG0480021121.01.T01
Chr4	exon	53802153	53802207	53802153	ID=MsG0480021121.01.T01:exon:7993;Parent=MsG0480021121.01.T01
Chr4	CDS	53809641	53809716	53809641	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53809111	53809175	53809111	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53808368	53808462	53808368	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53808172	53808263	53808172	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53807804	53808005	53807804	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53807633	53807705	53807633	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53807472	53807516	53807472	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53805537	53805598	53805537	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53803199	53803224	53803199	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53803059	53803119	53803059	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53802863	53802954	53802863	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53802495	53802696	53802495	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01
Chr4	CDS	53802153	53802207	53802153	ID=MsG0480021121.01.T01:cds;Parent=MsG0480021121.01.T01

Gene Sequence

>MsG0480021121.01.T01

ATGCAGAGGAACACTACAGAGGAGAGGAGATTCCTGCCTCCGCATAATAGATCTATTGGAATCGCAGAGGAGATTGGAGCAGTAATACTGCTCTCATGTTGCACATGTTTGTGGTATGGTGTGGATCGGATTGATGGAACTTATTTATTATATGCTTCTTTGCTATTTATGGAAGGTTTTATGAAACTTGTATTTGGGCTTGTAGGTGGATTTGACACGGCTCATGCTGCTGCTAGAGCCTATGATCGAGCTGCTATAAAGTTCAGGGGACTTGATGCTGACATCAATTTCAATCTAGTTGAGTATGAGGAAGATATGAATCAGGTTTATGGATCATATGATGGATTTTTCATCGTTTTGATTATTATCAATGATGATGAACAGATGAAGAATCTTTCAAAGGAGGAATTTGTGCACATTCTCCGTCGCCACAGTAACGGTTTCTCAAGGGGGAGTTCAAAATACAGAGGAGTAACACGTCACAAATGTGGTCGTTGGGAAGCTAGAATGGGACAACTCCTTGGCAAAAATACCAATTCAAGTTCCTGTTGGCCTATAAAAGGAAATGAAATTGATTTCACTTTCAGCTTATTCTCAAATGAGGTATATATATCTTGGGCTATTCGACAGCGAAGTAGAAGCTACAAGGGAGTAGGGACTGACATTATGAAATTTTTTATTCAATTTGTAGAAAGTAGAGTACTGCAGTGGGATTGTGGGAAGCAAGTTTATTTGGGTTTTATGAAACTTGTATTTGGGCTTGTAGGTGGATTTGACACGGCTCATGCTGCTGCTAGAGCCTATGATCGAGCTGCTATAAAGTTCAGGGGACTTGATGCTGACATCAATTTCAATCTAGTTGAGTATGAGGAAGATATGAATCAGGTTTATGGATCATATGATGGATTTTTCATCGTTTTGATTATTATCAATGATGATGAACAGATGAAGAATCTTTCAAAGGAGGAATTTGTGCACATTCTCCGTCGCCACAGTAACGGTTTCTCAAGGGGGAGTTCAAAATACAGAGGAGTAACACTTCACAAATGTGGTCGTTGGGAAGCTAGAATGGGACAACTCCTTGGCAAAAAGTATATATATCTTGGGCTATTCGACAGCGAAGTAGAAGCTGCAAGGTCCTTGTGA

Protein sequence

>MsG0480021121.01.T01

MQRNTTEERRFLPPHNRSIGIAEEIGAVILLSCCTCLWYGVDRIDGTYLLYASLLFMEGFMKLVFGLVGGFDTAHAAARAYDRAAIKFRGLDADINFNLVEYEEDMNQVYGSYDGFFIVLIIINDDEQMKNLSKEEFVHILRRHSNGFSRGSSKYRGVTRHKCGRWEARMGQLLGKNTNSSSCWPIKGNEIDFTFSLFSNEVYISWAIRQRSRSYKGVGTDIMKFFIQFVESRVLQWDCGKQVYLGFMKLVFGLVGGFDTAHAAARAYDRAAIKFRGLDADINFNLVEYEEDMNQVYGSYDGFFIVLIIINDDEQMKNLSKEEFVHILRRHSNGFSRGSSKYRGVTLHKCGRWEARMGQLLGKKYIYLGLFDSEVEAARSL*