Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021531.01.T01 | XP_024637178.2 | 53.191 | 141 | 21 | 3 | 2 | 100 | 14 | 151 | 2.31E-31 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021531.01.T01 | A0A396I9Z4 | 53.191 | 141 | 21 | 3 | 2 | 100 | 14 | 151 | 1.16e-31 | 127 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480021531.01 | MsG0780039443.01 | 0.804646 | 1.954917e-49 | 1.013387e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021531.01.T01 | MTR_4g074920 | 47.581 | 124 | 29 | 5 | 2 | 100 | 14 | 126 | 9.96e-23 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCATGCCCGCTTGGAGAAT+TGG | 0.280517 | 4:+59120361 | MsG0480021531.01.T01:CDS |
| CTCCAAGCGGGCATGCGAAT+TGG | 0.374070 | 4:-59120355 | None:intergenic |
| TCTTCGTTGACGGTGGTCTT+CGG | 0.436766 | 4:-59120082 | None:intergenic |
| CACCAATTCGCATGCCCGCT+TGG | 0.449781 | 4:+59120353 | MsG0480021531.01.T01:CDS |
| CAAAGAAGATCAGATAACTT+TGG | 0.503030 | 4:-59120056 | None:intergenic |
| AAGAATCAGCTAGATAAGTC+TGG | 0.514193 | 4:+59120315 | MsG0480021531.01.T01:CDS |
| AGTTCTGAAAGCGATGGAAG+TGG | 0.557517 | 4:+59120237 | MsG0480021531.01.T01:CDS |
| CATATGAAAATCTTCGTTGA+CGG | 0.562883 | 4:-59120092 | None:intergenic |
| CTTGATCCAATTCTCCAAGC+GGG | 0.566055 | 4:-59120367 | None:intergenic |
| GAGAATTGGATCAAGAGGAA+TGG | 0.589193 | 4:+59120375 | MsG0480021531.01.T01:CDS |
| GCTTGGAGAATTGGATCAAG+AGG | 0.610774 | 4:+59120370 | MsG0480021531.01.T01:CDS |
| ATGAAAATCTTCGTTGACGG+TGG | 0.615855 | 4:-59120089 | None:intergenic |
| AAACAAGTACATGTTAACGA+TGG | 0.650985 | 4:+59120273 | MsG0480021531.01.T01:CDS |
| TCTTGATCCAATTCTCCAAG+CGG | 0.660956 | 4:-59120368 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTATCAGAGAGATGTTTT+AGG | + | Chr4:59120119-59120138 | MsG0480021531.01.T01:intron | 25.0% |
| !!! | GGTCTAAAAAAGTATTTTGA+TGG | - | Chr4:59120038-59120057 | None:intergenic | 25.0% |
| AAACAAGTACATGTTAACGA+TGG | + | Chr4:59120273-59120292 | MsG0480021531.01.T01:CDS | 30.0% | |
| CAAAGAAGATCAGATAACTT+TGG | - | Chr4:59120059-59120078 | None:intergenic | 30.0% | |
| CATATGAAAATCTTCGTTGA+CGG | - | Chr4:59120095-59120114 | None:intergenic | 30.0% | |
| !! | ATGGAGCTAATTTTGATAAG+GGG | + | Chr4:59120292-59120311 | MsG0480021531.01.T01:CDS | 30.0% |
| !! | GATGGAGCTAATTTTGATAA+GGG | + | Chr4:59120291-59120310 | MsG0480021531.01.T01:CDS | 30.0% |
| !! | TTTGTCTCAGATAGTTTTAC+TGG | + | Chr4:59120163-59120182 | MsG0480021531.01.T01:intron | 30.0% |
| !!! | AAAAGACCTAGTTTTGTCTT+AGG | + | Chr4:59120193-59120212 | MsG0480021531.01.T01:intron | 30.0% |
| !!! | AATTTTAGTTCTGAAAGCGA+TGG | + | Chr4:59120231-59120250 | MsG0480021531.01.T01:CDS | 30.0% |
| AAGAATCAGCTAGATAAGTC+TGG | + | Chr4:59120315-59120334 | MsG0480021531.01.T01:CDS | 35.0% | |
| ACTACTCCTAAGACAAAACT+AGG | - | Chr4:59120202-59120221 | None:intergenic | 35.0% | |
| ! | CAACGAAGATTTTCATATGG+CGG | + | Chr4:59120096-59120115 | MsG0480021531.01.T01:CDS | 35.0% |
| ! | CGTCAACGAAGATTTTCATA+TGG | + | Chr4:59120093-59120112 | MsG0480021531.01.T01:CDS | 35.0% |
| !! | CGATGGAGCTAATTTTGATA+AGG | + | Chr4:59120290-59120309 | MsG0480021531.01.T01:CDS | 35.0% |
| ATGAAAATCTTCGTTGACGG+TGG | - | Chr4:59120092-59120111 | None:intergenic | 40.0% | |
| GAGAATTGGATCAAGAGGAA+TGG | + | Chr4:59120375-59120394 | MsG0480021531.01.T01:CDS | 40.0% | |
| TCTTGATCCAATTCTCCAAG+CGG | - | Chr4:59120371-59120390 | None:intergenic | 40.0% | |
| CTTGATCCAATTCTCCAAGC+GGG | - | Chr4:59120370-59120389 | None:intergenic | 45.0% | |
| GCTTGGAGAATTGGATCAAG+AGG | + | Chr4:59120370-59120389 | MsG0480021531.01.T01:CDS | 45.0% | |
| !! | AGTTCTGAAAGCGATGGAAG+TGG | + | Chr4:59120237-59120256 | MsG0480021531.01.T01:CDS | 45.0% |
| TCTTCGTTGACGGTGGTCTT+CGG | - | Chr4:59120085-59120104 | None:intergenic | 50.0% | |
| CACCAATTCGCATGCCCGCT+TGG | + | Chr4:59120353-59120372 | MsG0480021531.01.T01:CDS | 60.0% | |
| CGCATGCCCGCTTGGAGAAT+TGG | + | Chr4:59120361-59120380 | MsG0480021531.01.T01:CDS | 60.0% | |
| CTCCAAGCGGGCATGCGAAT+TGG | - | Chr4:59120358-59120377 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 59120017 | 59120422 | 59120017 | ID=MsG0480021531.01;Name=MsG0480021531.01 |
| Chr4 | mRNA | 59120017 | 59120422 | 59120017 | ID=MsG0480021531.01.T01;Parent=MsG0480021531.01;Name=MsG0480021531.01.T01;_AED=0.48;_eAED=0.64;_QI=0|0|0|1|1|1|2|0|102 |
| Chr4 | exon | 59120017 | 59120117 | 59120017 | ID=MsG0480021531.01.T01:exon:7292;Parent=MsG0480021531.01.T01 |
| Chr4 | exon | 59120215 | 59120422 | 59120215 | ID=MsG0480021531.01.T01:exon:7293;Parent=MsG0480021531.01.T01 |
| Chr4 | CDS | 59120017 | 59120117 | 59120017 | ID=MsG0480021531.01.T01:cds;Parent=MsG0480021531.01.T01 |
| Chr4 | CDS | 59120215 | 59120422 | 59120215 | ID=MsG0480021531.01.T01:cds;Parent=MsG0480021531.01.T01 |
| Gene Sequence |
| Protein sequence |
