AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021746.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021746.01.T01 MTR_4g075550 100.000 272 0 0 1 272 1 272 0.0 555
MsG0480021746.01.T01 MTR_3g113920 83.088 272 46 0 1 272 1 272 1.55e-172 477
MsG0480021746.01.T01 MTR_8g012595 49.077 271 135 3 1 268 1 271 1.39e-84 254
MsG0480021746.01.T01 MTR_8g012605 50.206 243 119 2 1 241 1 243 2.98e-83 251
MsG0480021746.01.T01 MTR_8g012615 52.821 195 91 1 75 268 9 203 3.49e-71 218
MsG0480021746.01.T01 MTR_8g012600 41.007 139 82 0 4 142 5 143 2.36e-31 118
MsG0480021746.01.T01 MTR_7g056337 34.061 229 130 8 47 269 2 215 5.60e-26 102
MsG0480021746.01.T01 MTR_8g087430 35.965 228 127 9 47 269 2 215 1.89e-25 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021746.01.T01 AT2G21190 89.552 268 28 0 5 272 2 269 0.0 498
MsG0480021746.01.T01 AT4G38790 88.806 268 30 0 5 272 6 273 8.17e-179 493
MsG0480021746.01.T01 AT1G75760 85.662 272 39 0 1 272 1 272 7.71e-176 486
MsG0480021746.01.T01 AT1G19970 80.147 272 54 0 1 272 1 272 2.36e-169 469
MsG0480021746.01.T01 AT1G75760 85.547 256 37 0 1 256 1 256 6.77e-165 457
MsG0480021746.01.T01 AT1G75760 85.185 216 32 0 1 216 1 216 5.78e-137 385
MsG0480021746.01.T01 AT1G19970 75.983 229 51 1 1 229 1 225 5.42e-132 373
MsG0480021746.01.T01 AT3G25160 61.194 268 103 1 2 268 4 271 2.07e-121 348
MsG0480021746.01.T01 AT3G25040 34.934 229 128 9 47 269 2 215 2.68e-29 110
MsG0480021746.01.T01 AT1G29330 35.371 229 127 10 47 269 2 215 3.33e-28 107
MsG0480021746.01.T01 AT1G29330 35.172 145 81 5 125 269 22 153 9.03e-18 78.6

Find 91 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCTTGGGGTATGGTTTA+TGG 0.172228 4:+63820096 MsG0480021746.01.T01:CDS
CGAGGTACCGTTATTTATTA+TGG 0.248467 4:+63820541 MsG0480021746.01.T01:three_prime_UTR
GTTCTTCTCTTTCTCTTTGT+TGG 0.263833 4:-63817537 None:intergenic
CTGTGCTCATTGGGTTCTTC+AGG 0.282833 4:+63819533 MsG0480021746.01.T01:CDS
CATTCACACCATACTTGATT+TGG 0.298660 4:+63818340 MsG0480021746.01.T01:CDS
CTGGGCATTCTGTGTATATC+TGG 0.298963 4:+63819307 MsG0480021746.01.T01:CDS
CTCAAGTTCTACCGAGTCTT+AGG 0.306962 4:+63820632 MsG0480021746.01.T01:three_prime_UTR
AAAATATAGCTGTCAATTCC+TGG 0.317259 4:-63818277 None:intergenic
CATGTTTGGTTACCCATGTT+TGG 0.328308 4:+63820413 MsG0480021746.01.T01:three_prime_UTR
GGACATTTGTTGGTTGCCTT+GGG 0.362241 4:+63820083 MsG0480021746.01.T01:CDS
CTTGGTGGAAGTGTAAAGTT+GGG 0.362567 4:+63820488 MsG0480021746.01.T01:three_prime_UTR
CATCGTTGATGATGATGTTA+AGG 0.365610 4:-63817609 None:intergenic
GATTCTTAAGCTGTGCTCAT+TGG 0.373170 4:+63819523 MsG0480021746.01.T01:CDS
TGGACATTTGTTGGTTGCCT+TGG 0.385662 4:+63820082 MsG0480021746.01.T01:CDS
ATTCTTAAGCTGTGCTCATT+GGG 0.386910 4:+63819524 MsG0480021746.01.T01:CDS
ACTTAATCAGGCATCATGTT+TGG 0.392971 4:+63820399 MsG0480021746.01.T01:three_prime_UTR
GCGAGAAAAGCCTTGACCTT+TGG 0.406284 4:-63817686 None:intergenic
ATCGTTGATGATGATGTTAA+GGG 0.410027 4:-63817608 None:intergenic
CTTCTCATCATATATTGAAC+AGG 0.417454 4:+63819279 MsG0480021746.01.T01:CDS
GAAAGTCGCCAAATCAAGTA+TGG 0.428500 4:-63818348 None:intergenic
TTGTTGGTTGCCTTGGGGTA+TGG 0.433590 4:+63820089 MsG0480021746.01.T01:CDS
GCTGAAGCTGTTCACTCGCT+TGG 0.434191 4:+63817782 MsG0480021746.01.T01:CDS
CTTGTTCTACGCCTTCCCTC+TGG 0.447485 4:+63820334 MsG0480021746.01.T01:CDS
AATATATGATGAGAAGTTGA+TGG 0.451706 4:-63819272 None:intergenic
CGTGATCATGAACGATAAAT+CGG 0.455398 4:-63817742 None:intergenic
AGAAAAGCCTTGACCTTTGG+TGG 0.458499 4:-63817683 None:intergenic
TTCTACCGAGTCTTAGGTTG+TGG 0.461994 4:+63820638 MsG0480021746.01.T01:three_prime_UTR
TCTCGCCGTCGTTTCCGGTA+TGG 0.465041 4:+63817703 MsG0480021746.01.T01:CDS
GTACCGTTATTTATTATGGT+TGG 0.473594 4:+63820545 MsG0480021746.01.T01:three_prime_UTR
ACGCCATCTCCACATGGGTC+AGG 0.477787 4:+63817654 MsG0480021746.01.T01:CDS
CCTTGGTGGAAGTGTAAAGT+TGG 0.487915 4:+63820487 MsG0480021746.01.T01:three_prime_UTR
AATCTGCTAAGATGAATGTC+TGG 0.487923 4:-63820148 None:intergenic
TGTTCTGCATGACCCGCAGT+TGG 0.489763 4:-63819349 None:intergenic
CTAACATGGGTAACCAAACA+TGG 0.496909 4:-63820426 None:intergenic
GTTCTGCATGACCCGCAGTT+GGG 0.500169 4:-63819348 None:intergenic
GAGAACAAGAGCGGCCATAC+CGG 0.503947 4:-63817717 None:intergenic
ATGAAGGAGAAGACTTGTGC+TGG 0.507531 4:+63817830 MsG0480021746.01.T01:CDS
AGATACCCGTGGACATTTGT+TGG 0.509868 4:+63820073 MsG0480021746.01.T01:CDS
TAAATTGAAGGCGAGTTACA+TGG 0.511519 4:+63818406 MsG0480021746.01.T01:CDS
GACTTTCGTGACCACGGTGT+GGG 0.516892 4:+63818364 MsG0480021746.01.T01:CDS
TCGCCTGACCCATGTGGAGA+TGG 0.520457 4:-63817657 None:intergenic
GTATGGTTTATGGCCGTCGA+TGG 0.522435 4:+63820106 MsG0480021746.01.T01:CDS
TCTGTTACTATTATGTCAAG+AGG 0.527285 4:+63820171 MsG0480021746.01.T01:CDS
AAATATAGCTGTCAATTCCT+GGG 0.533077 4:-63818276 None:intergenic
CGATCCACGCCATCTCCACA+TGG 0.539860 4:+63817648 MsG0480021746.01.T01:CDS
CGACTTTCGTGACCACGGTG+TGG 0.547169 4:+63818363 MsG0480021746.01.T01:CDS
GGCGAGTTACATGGAGGAGA+AGG 0.550641 4:+63818415 MsG0480021746.01.T01:CDS
AATACGTAATGAGCAGTGAA+TGG 0.555239 4:-63819483 None:intergenic
TTTCCAACCATAATAAATAA+CGG 0.555690 4:-63820548 None:intergenic
TTCAGTTCTACCCCAACTGC+GGG 0.557419 4:+63819337 MsG0480021746.01.T01:CDS
TTCTGCATGACCCGCAGTTG+GGG 0.562015 4:-63819347 None:intergenic
CTCGGTAGAACTTGAGCATG+TGG 0.573852 4:-63820625 None:intergenic
AGGATTATCACTCAAATCCC+AGG 0.579664 4:+63818259 MsG0480021746.01.T01:intron
AATGTACTCTAAGTCTAACA+TGG 0.583674 4:-63820440 None:intergenic
AGCAAGGCTAACACGGCACA+TGG 0.585861 4:-63819242 None:intergenic
AGCGGCCATACCGGAAACGA+CGG 0.593225 4:-63817708 None:intergenic
TTTCAGTTCTACCCCAACTG+CGG 0.594339 4:+63819336 MsG0480021746.01.T01:CDS
ATGAATGAGCAAGGCTAACA+CGG 0.596304 4:-63819249 None:intergenic
TCTTATCTATAAACTCATGA+AGG 0.599329 4:+63817814 MsG0480021746.01.T01:CDS
TGCAATCACACCACTCCAGA+GGG 0.600263 4:-63820349 None:intergenic
GCACAAGAACAAGACAAAGA+TGG 0.603634 4:-63817559 None:intergenic
GGCAACCAACAAATGTCCAC+GGG 0.610059 4:-63820078 None:intergenic
TGACCCATGTGGAGATGGCG+TGG 0.610245 4:-63817652 None:intergenic
AGGCAACCAACAAATGTCCA+CGG 0.615244 4:-63820079 None:intergenic
GCTCATTACGTATTTGCACT+CGG 0.615273 4:+63819492 MsG0480021746.01.T01:CDS
TAACTTTGCAATATACTATG+TGG 0.617993 4:+63818439 MsG0480021746.01.T01:CDS
GCGACAACCACCAAAGGTCA+AGG 0.619702 4:+63817676 MsG0480021746.01.T01:CDS
CACCAGGTGTTAGATACCCG+TGG 0.624596 4:+63820062 MsG0480021746.01.T01:intron
TCTACGCCTTCCCTCTGGAG+TGG 0.628133 4:+63820339 MsG0480021746.01.T01:CDS
GGTCAGGCGACAACCACCAA+AGG 0.628509 4:+63817670 MsG0480021746.01.T01:CDS
ATCACACCACTCCAGAGGGA+AGG 0.629698 4:-63820345 None:intergenic
GACATTTGTTGGTTGCCTTG+GGG 0.633865 4:+63820084 MsG0480021746.01.T01:CDS
ATGTACTCTAAGTCTAACAT+GGG 0.634914 4:-63820439 None:intergenic
AGTTGATGGATGAATGAGCA+AGG 0.649423 4:-63819258 None:intergenic
TTGGTGGAAGTGTAAAGTTG+GGG 0.651198 4:+63820489 MsG0480021746.01.T01:three_prime_UTR
CTGCAATCACACCACTCCAG+AGG 0.651756 4:-63820350 None:intergenic
TTTGGCGACTTTCGTGACCA+CGG 0.658314 4:+63818358 MsG0480021746.01.T01:CDS
GCGTGGATCGATGTTCTCTG+AGG 0.663389 4:-63817635 None:intergenic
CGGCCATAAACCATACCCCA+AGG 0.668614 4:-63820099 None:intergenic
TAACATGGGTAACCAAACAT+GGG 0.678898 4:-63820425 None:intergenic
ATAGACCACAACCTAAGACT+CGG 0.683423 4:-63820643 None:intergenic
GATCCACGCCATCTCCACAT+GGG 0.685742 4:+63817649 MsG0480021746.01.T01:CDS
CCAACTTTACACTTCCACCA+AGG 0.692281 4:-63820487 None:intergenic
GATCATGAACGATAAATCGG+AGG 0.692285 4:-63817739 None:intergenic
TAACATCATCATCAACGATG+AGG 0.692652 4:+63817612 MsG0480021746.01.T01:exon
TCTGAGATAAGAACCATCGA+CGG 0.702119 4:-63820119 None:intergenic
AAATCGGAGGAGAACAAGAG+CGG 0.713382 4:-63817726 None:intergenic
ATTGAAGGCGAGTTACATGG+AGG 0.718937 4:+63818409 MsG0480021746.01.T01:CDS
TGGTTGTCGCCTGACCCATG+TGG 0.729979 4:-63817663 None:intergenic
GCGGGTCATGCAGAACACCA+AGG 0.742034 4:+63819355 MsG0480021746.01.T01:CDS
CATGTATATAACCCACACCG+TGG 0.775290 4:-63818375 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTAAATAAAATATCATT+AGG - Chr4:63820295-63820314 None:intergenic 10.0%
!!! ATATATTTTTCTTTTGAAAA+TGG + Chr4:63819207-63819226 MsG0480021746.01.T01:intron 10.0%
!! AAAAAACATAAAACATTACA+TGG + Chr4:63818032-63818051 MsG0480021746.01.T01:intron 15.0%
!! AAAAACATAAAACATTACAT+GGG + Chr4:63818033-63818052 MsG0480021746.01.T01:intron 15.0%
!! AAGTTAAAAAGAAAATAAGA+GGG - Chr4:63820383-63820402 None:intergenic 15.0%
!! AATAATATGATTATGAAATC+TGG + Chr4:63818775-63818794 MsG0480021746.01.T01:intron 15.0%
!! TAAGTTAAAAAGAAAATAAG+AGG - Chr4:63820384-63820403 None:intergenic 15.0%
!! TGATTGATAAAACATAAAAA+GGG - Chr4:63817972-63817991 None:intergenic 15.0%
!! TTGATTGATAAAACATAAAA+AGG - Chr4:63817973-63817992 None:intergenic 15.0%
!!! ATTTTCTTTTTAACTTAATC+AGG + Chr4:63820387-63820406 MsG0480021746.01.T01:three_prime_UTR 15.0%
!!! TTTGAATTTTTTGTTTTCTT+GGG + Chr4:63817917-63817936 MsG0480021746.01.T01:intron 15.0%
!!! TTTTATGTTTTATCAATCAA+AGG + Chr4:63817973-63817992 MsG0480021746.01.T01:intron 15.0%
!!! TTTTTTATGTGAAATTGAAA+GGG - Chr4:63817869-63817888 None:intergenic 15.0%
!!! TTTTTTTATGTGAAATTGAA+AGG - Chr4:63817870-63817889 None:intergenic 15.0%
!! AAAATATATCAAGCTGATAA+TGG - Chr4:63819196-63819215 None:intergenic 20.0%
!! AGAACAAATAGTTAAAGATT+CGG - Chr4:63818698-63818717 None:intergenic 20.0%
!! TTGACAAAAAAATATTGACA+AGG - Chr4:63818507-63818526 None:intergenic 20.0%
!! TTTCCAACCATAATAAATAA+CGG - Chr4:63820551-63820570 None:intergenic 20.0%
!!! CTAAATATATTTTATGTTCG+AGG + Chr4:63820523-63820542 MsG0480021746.01.T01:three_prime_UTR 20.0%
!!! GTTTGAATTTTTTGTTTTCT+TGG + Chr4:63817916-63817935 MsG0480021746.01.T01:intron 20.0%
!!! TGTCAATATTTTTTTGTCAA+TGG + Chr4:63818507-63818526 MsG0480021746.01.T01:intron 20.0%
!!! TGTTTTTTCCTACTTTTTTA+TGG + Chr4:63818535-63818554 MsG0480021746.01.T01:intron 20.0%
!!! TTATTTAATTGCAGTGTTTT+CGG + Chr4:63820304-63820323 MsG0480021746.01.T01:intron 20.0%
!!! TTGCTTTAATGTGTTATTTT+TGG + Chr4:63818169-63818188 MsG0480021746.01.T01:intron 20.0%
! AATATATGATGAGAAGTTGA+TGG - Chr4:63819275-63819294 None:intergenic 25.0%
! AATCATATTATTCATGCAGA+AGG - Chr4:63818767-63818786 None:intergenic 25.0%
! ACAAAATGAAACAATTCAGA+AGG - Chr4:63819446-63819465 None:intergenic 25.0%
! CATCTTTACCATAAAAAAGT+AGG - Chr4:63818546-63818565 None:intergenic 25.0%
! GATGCAATCATATTTGAATA+CGG + Chr4:63819041-63819060 MsG0480021746.01.T01:intron 25.0%
! TAACTTTGCAATATACTATG+TGG + Chr4:63818439-63818458 MsG0480021746.01.T01:CDS 25.0%
! TAATTAGGAGATTACAGATT+CGG - Chr4:63819865-63819884 None:intergenic 25.0%
! TCTTATCTATAAACTCATGA+AGG + Chr4:63817814-63817833 MsG0480021746.01.T01:CDS 25.0%
! TTGCCCTTAAAAAAATTAGA+CGG + Chr4:63818104-63818123 MsG0480021746.01.T01:intron 25.0%
!! ATATATTGAACAGGATTTTC+TGG + Chr4:63819288-63819307 MsG0480021746.01.T01:CDS 25.0%
!! ATATCATATTGATAGTCATC+TGG + Chr4:63819941-63819960 MsG0480021746.01.T01:intron 25.0%
!! TATATTGAACAGGATTTTCT+GGG + Chr4:63819289-63819308 MsG0480021746.01.T01:CDS 25.0%
!! TATCATATTGATAGTCATCT+GGG + Chr4:63819942-63819961 MsG0480021746.01.T01:intron 25.0%
!! TGATTGCATCAGATTTTATT+TGG - Chr4:63819031-63819050 None:intergenic 25.0%
!! TTTAGGATGAAACAAACAAT+TGG - Chr4:63818471-63818490 None:intergenic 25.0%
!!! CATGATTCGTTTTAAATTGA+AGG + Chr4:63818394-63818413 MsG0480021746.01.T01:CDS 25.0%
!!! TGTTATTTTTGGATCATGAA+AGG + Chr4:63818180-63818199 MsG0480021746.01.T01:intron 25.0%
!!! TTTGTTTTGAATGATGTAAC+AGG + Chr4:63818239-63818258 MsG0480021746.01.T01:intron 25.0%
AAAATATAGCTGTCAATTCC+TGG - Chr4:63818280-63818299 None:intergenic 30.0%
AAATATAGCTGTCAATTCCT+GGG - Chr4:63818279-63818298 None:intergenic 30.0%
AAGGCAATGCGTTTAAATTT+AGG - Chr4:63818488-63818507 None:intergenic 30.0%
AATGTACTCTAAGTCTAACA+TGG - Chr4:63820443-63820462 None:intergenic 30.0%
AATTAGGAGATTACAGATTC+GGG - Chr4:63819864-63819883 None:intergenic 30.0%
ATACTATTCAGTATTTACCC+AGG - Chr4:63818814-63818833 None:intergenic 30.0%
ATGTACTCTAAGTCTAACAT+GGG - Chr4:63820442-63820461 None:intergenic 30.0%
ATGTCCATCATCTAATGTAT+GGG + Chr4:63819402-63819421 MsG0480021746.01.T01:intron 30.0%
CTTCTCATCATATATTGAAC+AGG + Chr4:63819279-63819298 MsG0480021746.01.T01:CDS 30.0%
GGCGTTAAAAATTTATACCT+TGG - Chr4:63819375-63819394 None:intergenic 30.0%
GTACCGTTATTTATTATGGT+TGG + Chr4:63820545-63820564 MsG0480021746.01.T01:three_prime_UTR 30.0%
TAAATTATTCCTCCTCCTAA+AGG + Chr4:63819123-63819142 MsG0480021746.01.T01:intron 30.0%
TAAATTTGCAACTTGATCAG+AGG - Chr4:63819594-63819613 None:intergenic 30.0%
TAACAAGCTATCATGCAAAA+TGG + Chr4:63818846-63818865 MsG0480021746.01.T01:intron 30.0%
TATGTCCATCATCTAATGTA+TGG + Chr4:63819401-63819420 MsG0480021746.01.T01:intron 30.0%
TATTTACCCAGGTTATTCTA+TGG - Chr4:63818803-63818822 None:intergenic 30.0%
TCTGTAATCTCCTAATTACA+TGG + Chr4:63819867-63819886 MsG0480021746.01.T01:intron 30.0%
TCTGTTACTATTATGTCAAG+AGG + Chr4:63820171-63820190 MsG0480021746.01.T01:CDS 30.0%
TGCCCTTAAAAAAATTAGAC+GGG + Chr4:63818105-63818124 MsG0480021746.01.T01:intron 30.0%
TGTTACAATGTTTGTGTGTT+GGG + Chr4:63819904-63819923 MsG0480021746.01.T01:intron 30.0%
TTAAAAAAATTAGACGGGTC+AGG + Chr4:63818110-63818129 MsG0480021746.01.T01:intron 30.0%
TTATTACTAACCTGCAACAT+TGG + Chr4:63818874-63818893 MsG0480021746.01.T01:intron 30.0%
TTCTTGGGTCTTTGTTAATT+GGG + Chr4:63817932-63817951 MsG0480021746.01.T01:intron 30.0%
TTTCTTGGGTCTTTGTTAAT+TGG + Chr4:63817931-63817950 MsG0480021746.01.T01:intron 30.0%
! AGATTTTTACCCCTTTAGAT+TGG + Chr4:63818079-63818098 MsG0480021746.01.T01:intron 30.0%
! AGGTATAAATTTTTAACGCC+AGG + Chr4:63819375-63819394 MsG0480021746.01.T01:intron 30.0%
! TCAGATCATAAACTTGCTTT+AGG + Chr4:63820698-63820717 MsG0480021746.01.T01:three_prime_UTR 30.0%
! TCTATATCTTTTCTTTGCAG+TGG + Chr4:63820661-63820680 MsG0480021746.01.T01:three_prime_UTR 30.0%
!! ATATATCAAGCTGATAATGG+TGG - Chr4:63819193-63819212 None:intergenic 30.0%
!! ATCGTTGATGATGATGTTAA+GGG - Chr4:63817611-63817630 None:intergenic 30.0%
!! GCTAATTTGATTTGTGTTTG+TGG + Chr4:63818146-63818165 MsG0480021746.01.T01:intron 30.0%
!! TTTCTTTTGAAAATGGTTGC+AGG + Chr4:63819214-63819233 MsG0480021746.01.T01:intron 30.0%
!!! ACAGCTAAGCCATTTTTTAA+AGG + Chr4:63819716-63819735 MsG0480021746.01.T01:intron 30.0%
!!! GACCCGTCTAATTTTTTTAA+GGG - Chr4:63818110-63818129 None:intergenic 30.0%
!!! TGACCCGTCTAATTTTTTTA+AGG - Chr4:63818111-63818130 None:intergenic 30.0%
AAAAGAAGAGGGGGTTTAAA+AGG - Chr4:63819071-63819090 None:intergenic 35.0%
AATACGTAATGAGCAGTGAA+TGG - Chr4:63819486-63819505 None:intergenic 35.0%
AATCTGCTAAGATGAATGTC+TGG - Chr4:63820151-63820170 None:intergenic 35.0%
ACAAAATCAAAGCCTTTAGG+AGG - Chr4:63819138-63819157 None:intergenic 35.0%
ACTTAATCAGGCATCATGTT+TGG + Chr4:63820399-63820418 MsG0480021746.01.T01:three_prime_UTR 35.0%
AGATCCCATACATTAGATGA+TGG - Chr4:63819409-63819428 None:intergenic 35.0%
AGTACAGGACTAAAAGAAGA+GGG - Chr4:63819082-63819101 None:intergenic 35.0%
ATATCATTAGGACAACAGCA+CGG - Chr4:63820283-63820302 None:intergenic 35.0%
ATATTCCACTCCTTGATCAA+CGG - Chr4:63819645-63819664 None:intergenic 35.0%
ATGCAGAAGGAAATTTGCAT+CGG - Chr4:63818754-63818773 None:intergenic 35.0%
ATTCTTAAGCTGTGCTCATT+GGG + Chr4:63819524-63819543 MsG0480021746.01.T01:CDS 35.0%
CACACAAACATTGTAACAGA+TGG - Chr4:63819902-63819921 None:intergenic 35.0%
CATTCACACCATACTTGATT+TGG + Chr4:63818340-63818359 MsG0480021746.01.T01:CDS 35.0%
CGTGATCATGAACGATAAAT+CGG - Chr4:63817745-63817764 None:intergenic 35.0%
CTGTTACAATGTTTGTGTGT+TGG + Chr4:63819903-63819922 MsG0480021746.01.T01:intron 35.0%
GCAAGAAATGGTATGTTAGT+AGG - Chr4:63820043-63820062 None:intergenic 35.0%
GGGAAAGATGACTCAAAAAA+TGG - Chr4:63819624-63819643 None:intergenic 35.0%
GTAATGCGAAAGATACTGAT+TGG + Chr4:63819834-63819853 MsG0480021746.01.T01:intron 35.0%
GTCACAAAATCAAAGCCTTT+AGG - Chr4:63819141-63819160 None:intergenic 35.0%
GTTCTTCTCTTTCTCTTTGT+TGG - Chr4:63817540-63817559 None:intergenic 35.0%
TAAATTGAAGGCGAGTTACA+TGG + Chr4:63818406-63818425 MsG0480021746.01.T01:CDS 35.0%
TAACATCATCATCAACGATG+AGG + Chr4:63817612-63817631 MsG0480021746.01.T01:exon 35.0%
TAACATGGGTAACCAAACAT+GGG - Chr4:63820428-63820447 None:intergenic 35.0%
TATCTTTCGCATTACAGACA+AGG - Chr4:63819829-63819848 None:intergenic 35.0%
! CACAGGTTGCCTTTAAAAAA+TGG - Chr4:63819728-63819747 None:intergenic 35.0%
! CTTTGAGGAAAAACACAACA+CGG - Chr4:63817584-63817603 None:intergenic 35.0%
! GATGTTAAGGGTTTTCTTTG+AGG - Chr4:63817599-63817618 None:intergenic 35.0%
! TAAGAATATGCAGAGAGTAC+AGG - Chr4:63819097-63819116 None:intergenic 35.0%
! TTTAATTGCAGTGTTTTCGG+AGG + Chr4:63820307-63820326 MsG0480021746.01.T01:intron 35.0%
! TTTATCACACCTTTTGCTGA+TGG + Chr4:63820231-63820250 MsG0480021746.01.T01:intron 35.0%
!! CATCGTTGATGATGATGTTA+AGG - Chr4:63817612-63817631 None:intergenic 35.0%
!! CGAGGTACCGTTATTTATTA+TGG + Chr4:63820541-63820560 MsG0480021746.01.T01:three_prime_UTR 35.0%
!! TTATTTGGTGATGTGTCACT+TGG - Chr4:63819016-63819035 None:intergenic 35.0%
!!! ATGCTTATTTTGTTCCTTGG+TGG + Chr4:63820473-63820492 MsG0480021746.01.T01:three_prime_UTR 35.0%
!!! CAGCTATATTTTTAGCTGTG+AGG + Chr4:63818288-63818307 MsG0480021746.01.T01:CDS 35.0%
!!! GACATGCTTATTTTGTTCCT+TGG + Chr4:63820470-63820489 MsG0480021746.01.T01:three_prime_UTR 35.0%
!!! GCTGTATTGTAGTTTTGTCA+TGG + Chr4:63818310-63818329 MsG0480021746.01.T01:CDS 35.0%
AACATACCATTTCTTGCACC+AGG + Chr4:63820046-63820065 MsG0480021746.01.T01:intron 40.0%
AAGGGCAATGCCAATCTAAA+GGG - Chr4:63818092-63818111 None:intergenic 40.0%
AGGATTATCACTCAAATCCC+AGG + Chr4:63818259-63818278 MsG0480021746.01.T01:intron 40.0%
ATAGACCACAACCTAAGACT+CGG - Chr4:63820646-63820665 None:intergenic 40.0%
ATATGCCTGCATACTAGACT+AGG + Chr4:63818930-63818949 MsG0480021746.01.T01:intron 40.0%
ATGAATGAGCAAGGCTAACA+CGG - Chr4:63819252-63819271 None:intergenic 40.0%
CAGCATGCACATGATTTACT+AGG + Chr4:63818970-63818989 MsG0480021746.01.T01:intron 40.0%
CATGTTTGGTTACCCATGTT+TGG + Chr4:63820413-63820432 MsG0480021746.01.T01:three_prime_UTR 40.0%
CTAACATGGGTAACCAAACA+TGG - Chr4:63820429-63820448 None:intergenic 40.0%
CTGGATCCATAGAATAACCT+GGG + Chr4:63818794-63818813 MsG0480021746.01.T01:intron 40.0%
GAAAGTCGCCAAATCAAGTA+TGG - Chr4:63818351-63818370 None:intergenic 40.0%
GAGTACAGGACTAAAAGAAG+AGG - Chr4:63819083-63819102 None:intergenic 40.0%
GATCATGAACGATAAATCGG+AGG - Chr4:63817742-63817761 None:intergenic 40.0%
GATTCTTAAGCTGTGCTCAT+TGG + Chr4:63819523-63819542 MsG0480021746.01.T01:CDS 40.0%
GCACAAGAACAAGACAAAGA+TGG - Chr4:63817562-63817581 None:intergenic 40.0%
GCTCATTACGTATTTGCACT+CGG + Chr4:63819492-63819511 MsG0480021746.01.T01:CDS 40.0%
GTACAGGACTAAAAGAAGAG+GGG - Chr4:63819081-63819100 None:intergenic 40.0%
TAAGGGCAATGCCAATCTAA+AGG - Chr4:63818093-63818112 None:intergenic 40.0%
TACAGGACTAAAAGAAGAGG+GGG - Chr4:63819080-63819099 None:intergenic 40.0%
TATGCCTGCATACTAGACTA+GGG + Chr4:63818931-63818950 MsG0480021746.01.T01:intron 40.0%
TATTCCACTCCTTGATCAAC+GGG - Chr4:63819644-63819663 None:intergenic 40.0%
TCTGGATCCATAGAATAACC+TGG + Chr4:63818793-63818812 MsG0480021746.01.T01:intron 40.0%
TGGGTCTTTGTTAATTGGGT+AGG + Chr4:63817936-63817955 MsG0480021746.01.T01:intron 40.0%
TGTATACTACCATTGCACAC+AGG - Chr4:63818651-63818670 None:intergenic 40.0%
! AAATCAAAGCCTTTAGGAGG+AGG - Chr4:63819135-63819154 None:intergenic 40.0%
! CTTGGTGGAAGTGTAAAGTT+GGG + Chr4:63820488-63820507 MsG0480021746.01.T01:three_prime_UTR 40.0%
! TCTGAGATAAGAACCATCGA+CGG - Chr4:63820122-63820141 None:intergenic 40.0%
! TTGGTGGAAGTGTAAAGTTG+GGG + Chr4:63820489-63820508 MsG0480021746.01.T01:three_prime_UTR 40.0%
!! AGTTGATGGATGAATGAGCA+AGG - Chr4:63819261-63819280 None:intergenic 40.0%
!! ATCAAGCTGATAATGGTGGA+AGG - Chr4:63819189-63819208 None:intergenic 40.0%
!! GAGTTTGCTGCCATGTAATT+AGG - Chr4:63819880-63819899 None:intergenic 40.0%
!! TAGATGATGGACATAGATCC+TGG - Chr4:63819396-63819415 None:intergenic 40.0%
!!! CTGTTTTTGCATCGACTCTT+TGG + Chr4:63819978-63819997 MsG0480021746.01.T01:intron 40.0%
AAATCGGAGGAGAACAAGAG+CGG - Chr4:63817729-63817748 None:intergenic 45.0%
AGAAAAGCCTTGACCTTTGG+TGG - Chr4:63817686-63817705 None:intergenic 45.0%
AGACTACAACGCATGCACAA+TGG - Chr4:63819785-63819804 None:intergenic 45.0%
AGATACCCGTGGACATTTGT+TGG + Chr4:63820073-63820092 MsG0480021746.01.T01:CDS 45.0%
AGGCAACCAACAAATGTCCA+CGG - Chr4:63820082-63820101 None:intergenic 45.0%
AGGGCAATGCCAATCTAAAG+GGG - Chr4:63818091-63818110 None:intergenic 45.0%
ATAAGCGCAACCAATGTTGC+AGG - Chr4:63818887-63818906 None:intergenic 45.0%
ATGAAGGAGAAGACTTGTGC+TGG + Chr4:63817830-63817849 MsG0480021746.01.T01:CDS 45.0%
ATTGAAGGCGAGTTACATGG+AGG + Chr4:63818409-63818428 MsG0480021746.01.T01:CDS 45.0%
CATGTATATAACCCACACCG+TGG - Chr4:63818378-63818397 None:intergenic 45.0%
CCAACTTTACACTTCCACCA+AGG - Chr4:63820490-63820509 None:intergenic 45.0%
CGTAAGTCACCATCAGCAAA+AGG - Chr4:63820243-63820262 None:intergenic 45.0%
CTAACACCTGGTGCAAGAAA+TGG - Chr4:63820055-63820074 None:intergenic 45.0%
CTCAAGTTCTACCGAGTCTT+AGG + Chr4:63820632-63820651 MsG0480021746.01.T01:three_prime_UTR 45.0%
CTGGGCATTCTGTGTATATC+TGG + Chr4:63819307-63819326 MsG0480021746.01.T01:CDS 45.0%
GACTACAACGCATGCACAAT+GGG - Chr4:63819784-63819803 None:intergenic 45.0%
GATATGTCACCTGTGTGCAA+TGG + Chr4:63818639-63818658 MsG0480021746.01.T01:intron 45.0%
GTCATCTTTCCCGTTGATCA+AGG + Chr4:63819632-63819651 MsG0480021746.01.T01:intron 45.0%
TGCATACTAGACTAGGGTCT+GGG + Chr4:63818937-63818956 MsG0480021746.01.T01:intron 45.0%
TTGCCTTGGGGTATGGTTTA+TGG + Chr4:63820096-63820115 MsG0480021746.01.T01:CDS 45.0%
TTTCAGTTCTACCCCAACTG+CGG + Chr4:63819336-63819355 MsG0480021746.01.T01:CDS 45.0%
! AAAGCTCTTGCCATTTGGTG+AGG + Chr4:63819752-63819771 MsG0480021746.01.T01:intron 45.0%
! AAGCTCTTGCCATTTGGTGA+GGG + Chr4:63819753-63819772 MsG0480021746.01.T01:intron 45.0%
! CCTTGGTGGAAGTGTAAAGT+TGG + Chr4:63820487-63820506 MsG0480021746.01.T01:three_prime_UTR 45.0%
! GACATTTGTTGGTTGCCTTG+GGG + Chr4:63820084-63820103 MsG0480021746.01.T01:CDS 45.0%
! GGACATTTGTTGGTTGCCTT+GGG + Chr4:63820083-63820102 MsG0480021746.01.T01:CDS 45.0%
! TGGACATTTGTTGGTTGCCT+TGG + Chr4:63820082-63820101 MsG0480021746.01.T01:CDS 45.0%
! TTCTACCGAGTCTTAGGTTG+TGG + Chr4:63820638-63820657 MsG0480021746.01.T01:three_prime_UTR 45.0%
!! CTTGGTTCTGTTGCAAGAAG+AGG - Chr4:63818998-63819017 None:intergenic 45.0%
!! CTTTGGTTGTGTAGCTTGCT+TGG + Chr4:63819995-63820014 MsG0480021746.01.T01:intron 45.0%
!! GCTCTAAAGCTCTTGCCATT+TGG + Chr4:63819747-63819766 MsG0480021746.01.T01:intron 45.0%
!!! ATAAAAAAAATTGATTTTTT+TGG + Chr4:63817881-63817900 MsG0480021746.01.T01:intron 5.0%
!!! TAAAAAAAATTGATTTTTTT+GGG + Chr4:63817882-63817901 MsG0480021746.01.T01:intron 5.0%
CAGACCCTAGTCTAGTATGC+AGG - Chr4:63818938-63818957 None:intergenic 50.0%
CTCGGTAGAACTTGAGCATG+TGG - Chr4:63820628-63820647 None:intergenic 50.0%
CTGCATACTAGACTAGGGTC+TGG + Chr4:63818936-63818955 MsG0480021746.01.T01:intron 50.0%
CTGTGCTCATTGGGTTCTTC+AGG + Chr4:63819533-63819552 MsG0480021746.01.T01:CDS 50.0%
CTTTCCCGTTGATCAAGGAG+TGG + Chr4:63819637-63819656 MsG0480021746.01.T01:intron 50.0%
GCATACTAGACTAGGGTCTG+GGG + Chr4:63818938-63818957 MsG0480021746.01.T01:intron 50.0%
GCGAGAAAAGCCTTGACCTT+TGG - Chr4:63817689-63817708 None:intergenic 50.0%
GGCAACCAACAAATGTCCAC+GGG - Chr4:63820081-63820100 None:intergenic 50.0%
GTATGGTTTATGGCCGTCGA+TGG + Chr4:63820106-63820125 MsG0480021746.01.T01:CDS 50.0%
TGCAATCACACCACTCCAGA+GGG - Chr4:63820352-63820371 None:intergenic 50.0%
TTCAGTTCTACCCCAACTGC+GGG + Chr4:63819337-63819356 MsG0480021746.01.T01:CDS 50.0%
TTTGGCGACTTTCGTGACCA+CGG + Chr4:63818358-63818377 MsG0480021746.01.T01:CDS 50.0%
! CAACGCATGCACAATGGGTT+TGG - Chr4:63819779-63819798 None:intergenic 50.0%
! TTGTTGGTTGCCTTGGGGTA+TGG + Chr4:63820089-63820108 MsG0480021746.01.T01:CDS 50.0%
!! TGGCAAGAGCTTTAGAGCAC+AGG - Chr4:63819745-63819764 None:intergenic 50.0%
AGCAAGGCTAACACGGCACA+TGG - Chr4:63819245-63819264 None:intergenic 55.0%
ATCACACCACTCCAGAGGGA+AGG - Chr4:63820348-63820367 None:intergenic 55.0%
CACCAGGTGTTAGATACCCG+TGG + Chr4:63820062-63820081 MsG0480021746.01.T01:intron 55.0%
CGGCCATAAACCATACCCCA+AGG - Chr4:63820102-63820121 None:intergenic 55.0%
CTGCAATCACACCACTCCAG+AGG - Chr4:63820353-63820372 None:intergenic 55.0%
CTTGTTCTACGCCTTCCCTC+TGG + Chr4:63820334-63820353 MsG0480021746.01.T01:CDS 55.0%
GACTTTCGTGACCACGGTGT+GGG + Chr4:63818364-63818383 MsG0480021746.01.T01:CDS 55.0%
GAGAACAAGAGCGGCCATAC+CGG - Chr4:63817720-63817739 None:intergenic 55.0%
GATCCACGCCATCTCCACAT+GGG + Chr4:63817649-63817668 MsG0480021746.01.T01:CDS 55.0%
GCGACAACCACCAAAGGTCA+AGG + Chr4:63817676-63817695 MsG0480021746.01.T01:CDS 55.0%
GCGTGGATCGATGTTCTCTG+AGG - Chr4:63817638-63817657 None:intergenic 55.0%
GCTGAAGCTGTTCACTCGCT+TGG + Chr4:63817782-63817801 MsG0480021746.01.T01:CDS 55.0%
GGCGAGTTACATGGAGGAGA+AGG + Chr4:63818415-63818434 MsG0480021746.01.T01:CDS 55.0%
GTCCACGGGTATCTAACACC+TGG - Chr4:63820067-63820086 None:intergenic 55.0%
GTTCTGCATGACCCGCAGTT+GGG - Chr4:63819351-63819370 None:intergenic 55.0%
TAGGACAACAGCACGGAGTC+AGG - Chr4:63820276-63820295 None:intergenic 55.0%
TGGGTTTGGCCCTCACCAAA+TGG - Chr4:63819765-63819784 None:intergenic 55.0%
TGTTCTGCATGACCCGCAGT+TGG - Chr4:63819352-63819371 None:intergenic 55.0%
TTCTGCATGACCCGCAGTTG+GGG - Chr4:63819350-63819369 None:intergenic 55.0%
! GCTTTTCTCGCCGTCGTTTC+CGG + Chr4:63817698-63817717 MsG0480021746.01.T01:CDS 55.0%
ACAACAGCACGGAGTCAGGC+AGG - Chr4:63820272-63820291 None:intergenic 60.0%
ACGCCATCTCCACATGGGTC+AGG + Chr4:63817654-63817673 MsG0480021746.01.T01:CDS 60.0%
AGCGGCCATACCGGAAACGA+CGG - Chr4:63817711-63817730 None:intergenic 60.0%
CGACTTTCGTGACCACGGTG+TGG + Chr4:63818363-63818382 MsG0480021746.01.T01:CDS 60.0%
CGATCCACGCCATCTCCACA+TGG + Chr4:63817648-63817667 MsG0480021746.01.T01:CDS 60.0%
GCGGGTCATGCAGAACACCA+AGG + Chr4:63819355-63819374 MsG0480021746.01.T01:CDS 60.0%
GGTCAGGCGACAACCACCAA+AGG + Chr4:63817670-63817689 MsG0480021746.01.T01:CDS 60.0%
TCGCCTGACCCATGTGGAGA+TGG - Chr4:63817660-63817679 None:intergenic 60.0%
TCTCGCCGTCGTTTCCGGTA+TGG + Chr4:63817703-63817722 MsG0480021746.01.T01:CDS 60.0%
TGACCCATGTGGAGATGGCG+TGG - Chr4:63817655-63817674 None:intergenic 60.0%
TGGTTGTCGCCTGACCCATG+TGG - Chr4:63817666-63817685 None:intergenic 60.0%
! TCTACGCCTTCCCTCTGGAG+TGG + Chr4:63820339-63820358 MsG0480021746.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 63817534 63820717 63817534 ID=MsG0480021746.01;Name=MsG0480021746.01
Chr4 mRNA 63817534 63820717 63817534 ID=MsG0480021746.01.T01;Parent=MsG0480021746.01;Name=MsG0480021746.01.T01;_AED=0.31;_eAED=0.31;_QI=95|1|1|1|1|1|6|351|272
Chr4 exon 63817534 63817851 63817534 ID=MsG0480021746.01.T01:exon:7002;Parent=MsG0480021746.01.T01
Chr4 exon 63818261 63818460 63818261 ID=MsG0480021746.01.T01:exon:7003;Parent=MsG0480021746.01.T01
Chr4 exon 63819236 63819376 63819236 ID=MsG0480021746.01.T01:exon:7004;Parent=MsG0480021746.01.T01
Chr4 exon 63819474 63819554 63819474 ID=MsG0480021746.01.T01:exon:7005;Parent=MsG0480021746.01.T01
Chr4 exon 63820068 63820192 63820068 ID=MsG0480021746.01.T01:exon:7006;Parent=MsG0480021746.01.T01
Chr4 exon 63820318 63820717 63820318 ID=MsG0480021746.01.T01:exon:7007;Parent=MsG0480021746.01.T01
Chr4 five_prime_UTR 63817534 63817628 63817534 ID=MsG0480021746.01.T01:five_prime_utr;Parent=MsG0480021746.01.T01
Chr4 CDS 63817629 63817851 63817629 ID=MsG0480021746.01.T01:cds;Parent=MsG0480021746.01.T01
Chr4 CDS 63818261 63818460 63818261 ID=MsG0480021746.01.T01:cds;Parent=MsG0480021746.01.T01
Chr4 CDS 63819236 63819376 63819236 ID=MsG0480021746.01.T01:cds;Parent=MsG0480021746.01.T01
Chr4 CDS 63819474 63819554 63819474 ID=MsG0480021746.01.T01:cds;Parent=MsG0480021746.01.T01
Chr4 CDS 63820068 63820192 63820068 ID=MsG0480021746.01.T01:cds;Parent=MsG0480021746.01.T01
Chr4 CDS 63820318 63820366 63820318 ID=MsG0480021746.01.T01:cds;Parent=MsG0480021746.01.T01
Chr4 three_prime_UTR 63820367 63820717 63820367 ID=MsG0480021746.01.T01:three_prime_utr;Parent=MsG0480021746.01.T01
Gene Sequence

>MsG0480021746.01.T01

ATGAGGCCTCAGAGAACATCGATCCACGCCATCTCCACATGGGTCAGGCGACAACCACCAAAGGTCAAGGCTTTTCTCGCCGTCGTTTCCGGTATGGCCGCTCTTGTTCTCCTCCGATTTATCGTTCATGATCACGATAATCTTTTCGTCGCTGCTGAAGCTGTTCACTCGCTTGGAATCTCTGTTCTTATCTATAAACTCATGAAGGAGAAGACTTGTGCTGGATTATCACTCAAATCCCAGGAATTGACAGCTATATTTTTAGCTGTGAGGCTGTATTGTAGTTTTGTCATGGAATATGACATTCACACCATACTTGATTTGGCGACTTTCGTGACCACGGTGTGGGTTATATACATGATTCGTTTTAAATTGAAGGCGAGTTACATGGAGGAGAAGGATAACTTTGCAATATACTATGTGGTGATACCATGTGCCGTGTTAGCCTTGCTCATTCATCCATCAACTTCTCATCATATATTGAACAGGATTTTCTGGGCATTCTGTGTATATCTGGAAGCTGTTTCAGTTCTACCCCAACTGCGGGTCATGCAGAACACCAAGATTGTTGAGCCATTCACTGCTCATTACGTATTTGCACTCGGCGTAGCAAGATTCTTAAGCTGTGCTCATTGGGTTCTTCAGGTGTTAGATACCCGTGGACATTTGTTGGTTGCCTTGGGGTATGGTTTATGGCCGTCGATGGTTCTTATCTCAGAAATTGTCCAGACATTCATCTTAGCAGATTTCTGTTACTATTATGTCAAGAGTGTTTTCGGAGGACAGCTTGTTCTACGCCTTCCCTCTGGAGTGGTGTGA
Protein sequence

>MsG0480021746.01.T01

MRPQRTSIHAISTWVRRQPPKVKAFLAVVSGMAALVLLRFIVHDHDNLFVAAEAVHSLGISVLIYKLMKEKTCAGLSLKSQELTAIFLAVRLYCSFVMEYDIHTILDLATFVTTVWVIYMIRFKLKASYMEEKDNFAIYYVVIPCAVLALLIHPSTSHHILNRIFWAFCVYLEAVSVLPQLRVMQNTKIVEPFTAHYVFALGVARFLSCAHWVLQVLDTRGHLLVALGYGLWPSMVLISEIVQTFILADFCYYYVKSVFGGQLVLRLPSGVV*