Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021982.01.T01 | XP_003607599.1 | 100 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 8.20E-43 | 145 |
| MsG0480021982.01.T02 | XP_003607599.1 | 100 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 8.20E-43 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021982.01.T01 | Q9LXT3 | 77.778 | 72 | 16 | 0 | 1 | 72 | 1 | 72 | 1.18E-35 | 120 |
| MsG0480021982.01.T02 | Q9LXT3 | 77.778 | 72 | 16 | 0 | 1 | 72 | 1 | 72 | 1.18E-35 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021982.01.T01 | B7FHC5 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 3.92e-43 | 145 |
| MsG0480021982.01.T02 | B7FHC5 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 3.92e-43 | 145 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0480021982.01.T01 | TR | MBF1 |
| MsG0480021982.01.T02 | TR | MBF1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0680035636.01 | MsG0480021982.01 | PPI |
| MsG0680035636.01 | MsG0480021982.01 | PPI |
| MsG0480021982.01 | MsG0480021982.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021982.01.T01 | MTR_4g080090 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 9.93e-47 | 145 |
| MsG0480021982.01.T01 | MTR_2g084220 | 88.235 | 68 | 8 | 0 | 5 | 72 | 3 | 70 | 5.14e-38 | 123 |
| MsG0480021982.01.T01 | MTR_4g103930 | 94.737 | 38 | 2 | 0 | 32 | 69 | 45 | 82 | 5.36e-18 | 73.2 |
| MsG0480021982.01.T01 | MTR_6g086280 | 49.275 | 69 | 35 | 0 | 4 | 72 | 9 | 77 | 3.53e-16 | 68.2 |
| MsG0480021982.01.T01 | MTR_6g086280 | 49.275 | 69 | 35 | 0 | 4 | 72 | 9 | 77 | 3.72e-16 | 68.2 |
| MsG0480021982.01.T01 | MTR_6g018330 | 69.565 | 46 | 14 | 0 | 2 | 47 | 19 | 64 | 1.27e-14 | 63.9 |
| MsG0480021982.01.T02 | MTR_4g080090 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 9.93e-47 | 145 |
| MsG0480021982.01.T02 | MTR_2g084220 | 88.235 | 68 | 8 | 0 | 5 | 72 | 3 | 70 | 5.14e-38 | 123 |
| MsG0480021982.01.T02 | MTR_4g103930 | 94.737 | 38 | 2 | 0 | 32 | 69 | 45 | 82 | 5.36e-18 | 73.2 |
| MsG0480021982.01.T02 | MTR_6g086280 | 49.275 | 69 | 35 | 0 | 4 | 72 | 9 | 77 | 3.53e-16 | 68.2 |
| MsG0480021982.01.T02 | MTR_6g086280 | 49.275 | 69 | 35 | 0 | 4 | 72 | 9 | 77 | 3.72e-16 | 68.2 |
| MsG0480021982.01.T02 | MTR_6g018330 | 69.565 | 46 | 14 | 0 | 2 | 47 | 19 | 64 | 1.27e-14 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021982.01.T01 | AT3G58680 | 77.778 | 72 | 16 | 0 | 1 | 72 | 1 | 72 | 1.21e-36 | 120 |
| MsG0480021982.01.T01 | AT2G42680 | 77.778 | 72 | 16 | 0 | 1 | 72 | 1 | 72 | 5.20e-35 | 115 |
| MsG0480021982.01.T01 | AT3G24500 | 47.143 | 70 | 35 | 1 | 5 | 72 | 7 | 76 | 1.24e-14 | 63.2 |
| MsG0480021982.01.T01 | AT3G24500 | 47.143 | 70 | 35 | 1 | 5 | 72 | 7 | 76 | 2.01e-14 | 63.5 |
| MsG0480021982.01.T02 | AT3G58680 | 77.778 | 72 | 16 | 0 | 1 | 72 | 1 | 72 | 1.21e-36 | 120 |
| MsG0480021982.01.T02 | AT2G42680 | 77.778 | 72 | 16 | 0 | 1 | 72 | 1 | 72 | 5.20e-35 | 115 |
| MsG0480021982.01.T02 | AT3G24500 | 47.143 | 70 | 35 | 1 | 5 | 72 | 7 | 76 | 1.24e-14 | 63.2 |
| MsG0480021982.01.T02 | AT3G24500 | 47.143 | 70 | 35 | 1 | 5 | 72 | 7 | 76 | 2.01e-14 | 63.5 |
Find 0 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAACATTAATTAATTAGTTT+TGG | - | Chr4:66798266-66798285 | None:intergenic | 10.0% |
| !!! | AACATTAATTAATTAGTTTT+GGG | - | Chr4:66798265-66798284 | None:intergenic | 10.0% |
| !! | TTAATTTCATATATTGATCA+TGG | + | Chr4:66798455-66798474 | MsG0480021982.01.T02:intron | 15.0% |
| !!! | ATCGTAGATAAAAATAATTT+TGG | + | Chr4:66798393-66798412 | MsG0480021982.01.T02:intron | 15.0% |
| !!! | TTAATGTTTTTTTTAACTCT+TGG | + | Chr4:66798277-66798296 | MsG0480021982.01.T02:intron | 15.0% |
| !!! | TTGATGATTATTTTGTATTA+GGG | + | Chr4:66798418-66798437 | MsG0480021982.01.T02:intron | 15.0% |
| !!! | TTTGATGATTATTTTGTATT+AGG | + | Chr4:66798417-66798436 | MsG0480021982.01.T02:intron | 15.0% |
| !!! | AATTAATTAGTTTTGGGAAG+AGG | - | Chr4:66798259-66798278 | None:intergenic | 25.0% |
| AACAAAGAGATAAACATGTC+AGG | + | Chr4:66798092-66798111 | MsG0480021982.01.T02:intron | 30.0% | |
| ! | GGTTTGTGTTTTGTGATATT+TGG | + | Chr4:66798476-66798495 | MsG0480021982.01.T02:intron | 30.0% |
| ! | TGTGTTTTGTGATATTTGGA+TGG | + | Chr4:66798480-66798499 | MsG0480021982.01.T02:intron | 30.0% |
| !!! | GTTTTTTCTGTGTCTGTTTA+GGG | - | Chr4:66798065-66798084 | None:intergenic | 30.0% |
| !!! | TGTTTTTTCTGTGTCTGTTT+AGG | - | Chr4:66798066-66798085 | None:intergenic | 30.0% |
| AGGCCATATTTCTCAAGATT+GGG | + | Chr4:66798118-66798137 | MsG0480021982.01.T02:CDS | 35.0% | |
| GATATTTGGATGGTGATTGT+TGG | + | Chr4:66798490-66798509 | MsG0480021982.01.T02:intron | 35.0% | |
| GTTCCCAATCTTGAGAAATA+TGG | - | Chr4:66798124-66798143 | None:intergenic | 35.0% | |
| TAGGCCATATTTCTCAAGAT+TGG | + | Chr4:66798117-66798136 | MsG0480021982.01.T02:CDS | 35.0% | |
| !! | TTTCTTGACGGTATCGATAT+CGG | - | Chr4:66798228-66798247 | None:intergenic | 35.0% |
| CGTATTGCGATTGTTGTAGT+TGG | + | Chr4:66798307-66798326 | MsG0480021982.01.T02:intron | 40.0% | |
| GAGATAAACATGTCAGGTCT+AGG | + | Chr4:66798098-66798117 | MsG0480021982.01.T02:intron | 40.0% | |
| GCACTTCATTGAACACTAAG+AGG | + | Chr4:66798566-66798585 | MsG0480021982.01.T02:CDS | 40.0% | |
| TTCATTGAACACTAAGAGGC+TGG | + | Chr4:66798570-66798589 | MsG0480021982.01.T02:CDS | 40.0% | |
| ! | TAGCTCAGTGAAGGTGTAAA+TGG | - | Chr4:66798038-66798057 | None:intergenic | 40.0% |
| GACGGCTTTCTCATCTTTCT+TGG | - | Chr4:66798183-66798202 | None:intergenic | 45.0% | |
| GGCTTTCTCATCTTTCTTGG+CGG | - | Chr4:66798180-66798199 | None:intergenic | 45.0% | |
| TTTCTCATCTTTCTTGGCGG+CGG | - | Chr4:66798177-66798196 | None:intergenic | 45.0% | |
| GAACACTAAGAGGCTGGATG+AGG | + | Chr4:66798576-66798595 | MsG0480021982.01.T02:CDS | 50.0% | |
| GAGGAGCGAACGTTTCTTGA+CGG | - | Chr4:66798240-66798259 | None:intergenic | 50.0% | |
| TAGGGTTCGTAGCTCAGTGA+AGG | - | Chr4:66798047-66798066 | None:intergenic | 50.0% | |
| GCTTTCTTGCGGATGACGAC+TGG | - | Chr4:66798146-66798165 | None:intergenic | 55.0% | |
| ATCTTTCTTGGCGGCGGCGT+TGG | - | Chr4:66798171-66798190 | None:intergenic | 60.0% | |
| TCTTTCTTGGCGGCGGCGTT+GGG | - | Chr4:66798170-66798189 | None:intergenic | 60.0% | |
| ! | CGGCGTTGGGAGCTTTCTTG+CGG | - | Chr4:66798157-66798176 | None:intergenic | 60.0% |
| !! | GACGGTATCGATATCGGCGC+CGG | - | Chr4:66798222-66798241 | None:intergenic | 60.0% |
| !! | TATCGATATCGGCGCCGGCA+CGG | - | Chr4:66798217-66798236 | None:intergenic | 60.0% |
| GTCAACGCCGCTCGCCGTGC+CGG | + | Chr4:66798200-66798219 | MsG0480021982.01.T02:CDS | 75.0% | |
| ! | GGCACGGCGAGCGGCGTTGA+CGG | - | Chr4:66798201-66798220 | None:intergenic | 75.0% |
| !!! | ATCGGCGCCGGCACGGCGAG+CGG | - | Chr4:66798210-66798229 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 66798008 | 66798612 | 66798008 | ID=MsG0480021982.01;Name=MsG0480021982.01 |
| Chr4 | mRNA | 66798008 | 66798612 | 66798008 | ID=MsG0480021982.01.T01;Parent=MsG0480021982.01;Name=MsG0480021982.01.T01;_AED=0.48;_eAED=0.48;_QI=99|1|1|1|0.33|0.25|4|0|73 |
| Chr4 | five_prime_UTR | 66798008 | 66798106 | 66798008 | ID=MsG0480021982.01.T01:five_prime_utr;Parent=MsG0480021982.01.T01 |
| Chr4 | CDS | 66798107 | 66798248 | 66798107 | ID=MsG0480021982.01.T01:cds;Parent=MsG0480021982.01.T01 |
| Chr4 | CDS | 66798533 | 66798612 | 66798533 | ID=MsG0480021982.01.T01:cds;Parent=MsG0480021982.01.T01 |
| Chr4 | mRNA | 66798008 | 66798612 | 66798008 | ID=MsG0480021982.01.T02;Parent=MsG0480021982.01;Name=MsG0480021982.01.T02;_AED=0.45;_eAED=0.45;_QI=99|1|1|1|0.5|0.33|3|0|73 |
| Chr4 | five_prime_UTR | 66798008 | 66798106 | 66798008 | ID=MsG0480021982.01.T02:five_prime_utr;Parent=MsG0480021982.01.T02 |
| Chr4 | CDS | 66798107 | 66798248 | 66798107 | ID=MsG0480021982.01.T02:cds;Parent=MsG0480021982.01.T02 |
| Chr4 | CDS | 66798533 | 66798612 | 66798533 | ID=MsG0480021982.01.T02:cds;Parent=MsG0480021982.01.T02 |
| Gene Sequence |
| Protein sequence |
