Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022077.01.T01 | RYQ86459.1 | 71.053 | 114 | 30 | 1 | 8 | 118 | 94 | 207 | 3.51E-51 | 171 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022077.01.T01 | O94417 | 36.842 | 114 | 49 | 1 | 5 | 118 | 216 | 306 | 6.43E-16 | 74.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022077.01.T01 | A0A444X9T4 | 71.053 | 114 | 30 | 1 | 8 | 118 | 94 | 207 | 1.68e-51 | 171 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048826.01 | MsG0480022077.01 | 0.801329 | 9.476808e-49 | 4.513413e-46 |
| MsG0480022077.01 | MsG0780041507.01 | 0.808641 | 2.803428e-50 | 1.611352e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022077.01.T01 | MTR_8g030840 | 74.561 | 114 | 26 | 1 | 8 | 118 | 418 | 531 | 1.99e-54 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022077.01.T01 | AT1G31870 | 57.895 | 114 | 45 | 1 | 8 | 118 | 448 | 561 | 2.78e-39 | 138 |
| MsG0480022077.01.T01 | AT1G31870 | 57.895 | 114 | 45 | 1 | 8 | 118 | 448 | 561 | 2.78e-39 | 138 |
Find 41 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGCTTATGGATGGGTTAT+AGG | 0.247079 | 4:-67824653 | MsG0480022077.01.T01:three_prime_UTR |
| GCCTTATCCCATATCGATTA+GGG | 0.298324 | 4:+67824920 | None:intergenic |
| AAATAATGAGCTAGGGCTTA+TGG | 0.334371 | 4:-67824665 | MsG0480022077.01.T01:three_prime_UTR |
| ACCTTTACTAAATGTTTCAT+AGG | 0.355940 | 4:+67825149 | None:intergenic |
| CTCTTCAACCAGCTGTGATC+TGG | 0.363715 | 4:+67824958 | None:intergenic |
| TTCTGCAATTTATTAAGTTC+AGG | 0.364688 | 4:+67825194 | None:intergenic |
| TGCAGAAGGAGAAGGTACTA+TGG | 0.381597 | 4:-67825178 | MsG0480022077.01.T01:CDS |
| GGCCTTATCCCATATCGATT+AGG | 0.397775 | 4:+67824919 | None:intergenic |
| GGGCTTATGGATGGGTTATA+GGG | 0.408450 | 4:-67824652 | MsG0480022077.01.T01:three_prime_UTR |
| AGAGCCTGACCTTCCGAACT+TGG | 0.414914 | 4:-67825022 | MsG0480022077.01.T01:CDS |
| GAGCCTGACCTTCCGAACTT+GGG | 0.455564 | 4:-67825021 | MsG0480022077.01.T01:CDS |
| GGATAAGGCCGGGACGACAC+TGG | 0.471920 | 4:-67824906 | MsG0480022077.01.T01:CDS |
| TGATATGTAAATAATGAGCT+AGG | 0.483090 | 4:-67824673 | MsG0480022077.01.T01:exon |
| CCCCTAATCGATATGGGATA+AGG | 0.486802 | 4:-67824921 | MsG0480022077.01.T01:CDS |
| ATTATTATTAGTGTATATCT+CGG | 0.495061 | 4:+67824576 | None:intergenic |
| AAGTTCGGAAGGTCAGGCTC+TGG | 0.502945 | 4:+67825024 | None:intergenic |
| GATAAGGCCGGGACGACACT+GGG | 0.506196 | 4:-67824905 | MsG0480022077.01.T01:CDS |
| GTTATAGGGTATACTATACT+TGG | 0.508055 | 4:-67824638 | MsG0480022077.01.T01:three_prime_UTR |
| GAGACAAGCTGAAGAGAAAG+AGG | 0.520542 | 4:-67824715 | MsG0480022077.01.T01:CDS |
| AGCCTGACCTTCCGAACTTG+GGG | 0.530476 | 4:-67825020 | MsG0480022077.01.T01:CDS |
| AATGAGCTAGGGCTTATGGA+TGG | 0.531529 | 4:-67824661 | MsG0480022077.01.T01:three_prime_UTR |
| TTTACATATCAGACACTGAC+CGG | 0.538250 | 4:+67824685 | None:intergenic |
| TCCTATGAAACATTTAGTAA+AGG | 0.547545 | 4:-67825150 | MsG0480022077.01.T01:intron |
| AAGATATTCCAGATCACAGC+TGG | 0.549487 | 4:-67824966 | MsG0480022077.01.T01:CDS |
| TAATCGATATGGGATAAGGC+CGG | 0.549589 | 4:-67824917 | MsG0480022077.01.T01:CDS |
| ACACCATCCCAGTGTCGTCC+CGG | 0.550677 | 4:+67824898 | None:intergenic |
| CAGATCACAGCTGGTTGAAG+AGG | 0.552603 | 4:-67824957 | MsG0480022077.01.T01:CDS |
| AATCGATATGGGATAAGGCC+GGG | 0.564665 | 4:-67824916 | MsG0480022077.01.T01:CDS |
| GCAGAAGGAGAAGGTACTAT+GGG | 0.566961 | 4:-67825177 | MsG0480022077.01.T01:CDS |
| TAATAAATTGCAGAAGGAGA+AGG | 0.576609 | 4:-67825186 | MsG0480022077.01.T01:CDS |
| CTGTGATCTGGAATATCTTG+AGG | 0.579188 | 4:+67824970 | None:intergenic |
| GATGGTGTCGATCGCAGTAA+TGG | 0.580754 | 4:-67824883 | MsG0480022077.01.T01:intron |
| GATATGTAAATAATGAGCTA+GGG | 0.583876 | 4:-67824672 | MsG0480022077.01.T01:exon |
| TGAACTTAATAAATTGCAGA+AGG | 0.587762 | 4:-67825192 | MsG0480022077.01.T01:CDS |
| ATGAGCTAGGGCTTATGGAT+GGG | 0.591031 | 4:-67824660 | MsG0480022077.01.T01:three_prime_UTR |
| AGATCACAGCTGGTTGAAGA+GGG | 0.601625 | 4:-67824956 | MsG0480022077.01.T01:CDS |
| AAGAGAAAGAGGCTTATCTC+CGG | 0.607781 | 4:-67824704 | MsG0480022077.01.T01:CDS |
| AGGCCGGGACGACACTGGGA+TGG | 0.633533 | 4:-67824901 | MsG0480022077.01.T01:CDS |
| CTTATCCCATATCGATTAGG+GGG | 0.645050 | 4:+67824922 | None:intergenic |
| GCCTGACCTTCCGAACTTGG+GGG | 0.661087 | 4:-67825019 | MsG0480022077.01.T01:CDS |
| CCTTATCCCATATCGATTAG+GGG | 0.662538 | 4:+67824921 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTATTAGTGTATATCT+CGG | + | Chr4:67825211-67825230 | None:intergenic | 15.0% |
| !! | AAAATCCAAGTATAAAAATC+TGG | + | Chr4:67824942-67824961 | None:intergenic | 20.0% |
| !! | AACATTTCTCATTATAATAC+AGG | - | Chr4:67824967-67824986 | MsG0480022077.01.T01:CDS | 20.0% |
| !! | ACATTTCTCATTATAATACA+GGG | - | Chr4:67824968-67824987 | MsG0480022077.01.T01:CDS | 20.0% |
| !! | TAATTGACATGAAAAAACAT+TGG | + | Chr4:67824719-67824738 | None:intergenic | 20.0% |
| ! | ACCTTTACTAAATGTTTCAT+AGG | + | Chr4:67824638-67824657 | None:intergenic | 25.0% |
| ! | GATATGTAAATAATGAGCTA+GGG | - | Chr4:67825112-67825131 | MsG0480022077.01.T01:intron | 25.0% |
| ! | TCCTATGAAACATTTAGTAA+AGG | - | Chr4:67824634-67824653 | MsG0480022077.01.T01:three_prime_UTR | 25.0% |
| ! | TGAACTTAATAAATTGCAGA+AGG | - | Chr4:67824592-67824611 | MsG0480022077.01.T01:three_prime_UTR | 25.0% |
| ! | TGATATGTAAATAATGAGCT+AGG | - | Chr4:67825111-67825130 | MsG0480022077.01.T01:intron | 25.0% |
| ! | TTCTGCAATTTATTAAGTTC+AGG | + | Chr4:67824593-67824612 | None:intergenic | 25.0% |
| AGAGCTGAAGTCAATAATTT+AGG | + | Chr4:67825001-67825020 | None:intergenic | 30.0% | |
| GTTATAGGGTATACTATACT+TGG | - | Chr4:67825146-67825165 | MsG0480022077.01.T01:intron | 30.0% | |
| TAATAAATTGCAGAAGGAGA+AGG | - | Chr4:67824598-67824617 | MsG0480022077.01.T01:three_prime_UTR | 30.0% | |
| ! | ATCAGCCAGATTTTTATACT+TGG | - | Chr4:67824934-67824953 | MsG0480022077.01.T01:CDS | 30.0% |
| AAATAATGAGCTAGGGCTTA+TGG | - | Chr4:67825119-67825138 | MsG0480022077.01.T01:intron | 35.0% | |
| TGCAATGCAACTCTAATTGT+TGG | - | Chr4:67824660-67824679 | MsG0480022077.01.T01:three_prime_UTR | 35.0% | |
| TGTTGGACACTTACAATGAA+GGG | - | Chr4:67824677-67824696 | MsG0480022077.01.T01:exon | 35.0% | |
| TTGTTGGACACTTACAATGA+AGG | - | Chr4:67824676-67824695 | MsG0480022077.01.T01:exon | 35.0% | |
| TTTACATATCAGACACTGAC+CGG | + | Chr4:67825102-67825121 | None:intergenic | 35.0% | |
| AAGAGAAAGAGGCTTATCTC+CGG | - | Chr4:67825080-67825099 | MsG0480022077.01.T01:intron | 40.0% | |
| AAGATATTCCAGATCACAGC+TGG | - | Chr4:67824818-67824837 | MsG0480022077.01.T01:intron | 40.0% | |
| TAATCGATATGGGATAAGGC+CGG | - | Chr4:67824867-67824886 | MsG0480022077.01.T01:intron | 40.0% | |
| ! | CCTTATCCCATATCGATTAG+GGG | + | Chr4:67824866-67824885 | None:intergenic | 40.0% |
| ! | CTGTGATCTGGAATATCTTG+AGG | + | Chr4:67824817-67824836 | None:intergenic | 40.0% |
| ! | CTTATCCCATATCGATTAGG+GGG | + | Chr4:67824865-67824884 | None:intergenic | 40.0% |
| ! | GCCTTATCCCATATCGATTA+GGG | + | Chr4:67824867-67824886 | None:intergenic | 40.0% |
| AAGCTCCCCCTAATCGATAT+GGG | - | Chr4:67824857-67824876 | MsG0480022077.01.T01:intron | 45.0% | |
| AATCGATATGGGATAAGGCC+GGG | - | Chr4:67824868-67824887 | MsG0480022077.01.T01:intron | 45.0% | |
| AATGAGCTAGGGCTTATGGA+TGG | - | Chr4:67825123-67825142 | MsG0480022077.01.T01:intron | 45.0% | |
| AGATCACAGCTGGTTGAAGA+GGG | - | Chr4:67824828-67824847 | MsG0480022077.01.T01:intron | 45.0% | |
| ATGAGCTAGGGCTTATGGAT+GGG | - | Chr4:67825124-67825143 | MsG0480022077.01.T01:intron | 45.0% | |
| CCCCTAATCGATATGGGATA+AGG | - | Chr4:67824863-67824882 | MsG0480022077.01.T01:intron | 45.0% | |
| GAGACAAGCTGAAGAGAAAG+AGG | - | Chr4:67825069-67825088 | MsG0480022077.01.T01:intron | 45.0% | |
| TCATCACTTTCCCCCAAGTT+CGG | + | Chr4:67824778-67824797 | None:intergenic | 45.0% | |
| ! | GGCCTTATCCCATATCGATT+AGG | + | Chr4:67824868-67824887 | None:intergenic | 45.0% |
| !! | AGGGCTTATGGATGGGTTAT+AGG | - | Chr4:67825131-67825150 | MsG0480022077.01.T01:intron | 45.0% |
| !! | GCAGAAGGAGAAGGTACTAT+GGG | - | Chr4:67824607-67824626 | MsG0480022077.01.T01:three_prime_UTR | 45.0% |
| !! | GGGCTTATGGATGGGTTATA+GGG | - | Chr4:67825132-67825151 | MsG0480022077.01.T01:intron | 45.0% |
| !! | TGCAGAAGGAGAAGGTACTA+TGG | - | Chr4:67824606-67824625 | MsG0480022077.01.T01:three_prime_UTR | 45.0% |
| CAGATCACAGCTGGTTGAAG+AGG | - | Chr4:67824827-67824846 | MsG0480022077.01.T01:intron | 50.0% | |
| CCGCTCAAAACCTACAAGCA+GGG | + | Chr4:67825026-67825045 | None:intergenic | 50.0% | |
| CGCTCAAAACCTACAAGCAG+GGG | + | Chr4:67825025-67825044 | None:intergenic | 50.0% | |
| CTCTTCAACCAGCTGTGATC+TGG | + | Chr4:67824829-67824848 | None:intergenic | 50.0% | |
| GAAGCTCCCCCTAATCGATA+TGG | - | Chr4:67824856-67824875 | MsG0480022077.01.T01:intron | 50.0% | |
| !! | GATGGTGTCGATCGCAGTAA+TGG | - | Chr4:67824901-67824920 | MsG0480022077.01.T01:CDS | 50.0% |
| !!! | CCCTGCTTGTAGGTTTTGAG+CGG | - | Chr4:67825023-67825042 | MsG0480022077.01.T01:CDS | 50.0% |
| !!! | CCTGCTTGTAGGTTTTGAGC+GGG | - | Chr4:67825024-67825043 | MsG0480022077.01.T01:CDS | 50.0% |
| AAGTTCGGAAGGTCAGGCTC+TGG | + | Chr4:67824763-67824782 | None:intergenic | 55.0% | |
| AGAGCCTGACCTTCCGAACT+TGG | - | Chr4:67824762-67824781 | MsG0480022077.01.T01:intron | 55.0% | |
| AGCCTGACCTTCCGAACTTG+GGG | - | Chr4:67824764-67824783 | MsG0480022077.01.T01:intron | 55.0% | |
| CACTTTCCCCCAAGTTCGGA+AGG | + | Chr4:67824774-67824793 | None:intergenic | 55.0% | |
| CCCGCTCAAAACCTACAAGC+AGG | + | Chr4:67825027-67825046 | None:intergenic | 55.0% | |
| GAGCCTGACCTTCCGAACTT+GGG | - | Chr4:67824763-67824782 | MsG0480022077.01.T01:intron | 55.0% | |
| TCAGCTCTTCCCCTGCTTGT+AGG | - | Chr4:67825013-67825032 | MsG0480022077.01.T01:CDS | 55.0% | |
| ACACCATCCCAGTGTCGTCC+CGG | + | Chr4:67824889-67824908 | None:intergenic | 60.0% | |
| GATAAGGCCGGGACGACACT+GGG | - | Chr4:67824879-67824898 | MsG0480022077.01.T01:intron | 60.0% | |
| GCCTGACCTTCCGAACTTGG+GGG | - | Chr4:67824765-67824784 | MsG0480022077.01.T01:intron | 60.0% | |
| TCCCCCAAGTTCGGAAGGTC+AGG | + | Chr4:67824769-67824788 | None:intergenic | 60.0% | |
| GGATAAGGCCGGGACGACAC+TGG | - | Chr4:67824878-67824897 | MsG0480022077.01.T01:intron | 65.0% | |
| AGGCCGGGACGACACTGGGA+TGG | - | Chr4:67824883-67824902 | MsG0480022077.01.T01:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 67824557 | 67825249 | 67824557 | ID=MsG0480022077.01;Name=MsG0480022077.01 |
| Chr4 | mRNA | 67824557 | 67825249 | 67824557 | ID=MsG0480022077.01.T01;Parent=MsG0480022077.01;Name=MsG0480022077.01.T01;_AED=0.40;_eAED=0.43;_QI=0|1|0.66|1|1|1|3|129|118 |
| Chr4 | exon | 67824557 | 67824771 | 67824557 | ID=MsG0480022077.01.T01:exon:8384;Parent=MsG0480022077.01.T01 |
| Chr4 | exon | 67824884 | 67825055 | 67824884 | ID=MsG0480022077.01.T01:exon:8383;Parent=MsG0480022077.01.T01 |
| Chr4 | exon | 67825151 | 67825249 | 67825151 | ID=MsG0480022077.01.T01:exon:8382;Parent=MsG0480022077.01.T01 |
| Chr4 | CDS | 67825151 | 67825249 | 67825151 | ID=MsG0480022077.01.T01:cds;Parent=MsG0480022077.01.T01 |
| Chr4 | CDS | 67824884 | 67825055 | 67824884 | ID=MsG0480022077.01.T01:cds;Parent=MsG0480022077.01.T01 |
| Chr4 | CDS | 67824686 | 67824771 | 67824686 | ID=MsG0480022077.01.T01:cds;Parent=MsG0480022077.01.T01 |
| Chr4 | three_prime_UTR | 67824557 | 67824685 | 67824557 | ID=MsG0480022077.01.T01:three_prime_utr;Parent=MsG0480022077.01.T01 |
| Gene Sequence |
| Protein sequence |
