AlfalfaGEDB | MsG0480022375.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022375.01

KEGG: -
GO: -
PFAM: PF05678

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022375.01.T01	XP_013457018.1	94.466	253	5	3	1	253	1	244	1.32E-157	447

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022375.01.T01	Q5M750	55.512	254	64	12	19	242	4	238	4.85E-62	198

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022375.01.T01	A0A072UMN6	94.466	253	5	3	1	253	1	244	6.32e-158	447

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004714.01	MsG0480022375.01	-0.822126	2.799140e-53	2.322062e-50
MsG0180005636.01	MsG0480022375.01	0.801861	7.373455e-49	3.559308e-46
MsG0480022375.01	MsG0880046158.01	0.839449	1.573708e-57	2.174067e-54
MsG0380016481.01	MsG0480022375.01	0.817342	3.465876e-52	2.517376e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022375.01.T01	MTR_4g088695	94.466	253	5	3	1	253	1	244	1.60e-161	447
MsG0480022375.01.T01	MTR_2g013950	66.834	199	46	8	49	242	69	252	1.20e-68	213
MsG0480022375.01.T01	MTR_2g079120	53.271	214	62	8	46	242	49	241	7.98e-51	166

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022375.01.T01	AT1G28280	55.078	256	66	12	19	244	4	240	3.88e-63	198
MsG0480022375.01.T01	AT1G28280	55.512	254	64	12	19	242	4	238	4.94e-63	198
MsG0480022375.01.T01	AT3G15300	45.249	221	79	12	36	241	21	214	6.93e-37	130
MsG0480022375.01.T01	AT5G53830	48.095	210	66	10	59	240	45	239	3.85e-32	118
MsG0480022375.01.T01	AT2G33780	45.596	193	72	9	53	238	26	192	6.66e-32	116
MsG0480022375.01.T01	AT1G80450	34.444	180	84	8	63	240	15	162	4.91e-12	63.2

Find 58 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATACTAGGAGACAAAATTTC+AGG	0.211265	4:+71727696	None:intergenic
TCAATGTTCAAAACTGGTTT+AGG	0.256425	4:+71727561	None:intergenic
AACTTCTCCAAGAGCTCGTT+TGG	0.269044	4:-71727433	MsG0480022375.01.T01:CDS
TCGGATCTGGTGACTGGTTT+TGG	0.283666	4:+71727999	None:intergenic
TGAACAAATGTTGTCGGGTA+TGG	0.340930	4:+71727966	None:intergenic
TCCGGTATAAGAGGTGTAAC+AGG	0.351979	4:+71727645	None:intergenic
TCAGCTTCAATGTTCAAAAC+TGG	0.358597	4:+71727555	None:intergenic
AAGAGAAAACAGGAAGTAAT+GGG	0.372681	4:+71727757	None:intergenic
AAACAGGAAGTAATGGGTTT+AGG	0.379009	4:+71727763	None:intergenic
TTTGAATTTGAAGAGAAAAC+AGG	0.388342	4:+71727747	None:intergenic
CAAGTTGTTCAAATGTTAAC+CGG	0.392337	4:-71727921	MsG0480022375.01.T01:CDS
GGAAGAGAAGTGTTGTTTCT+TGG	0.397199	4:+71728113	None:intergenic
GAGACGGTGGTTCTGTTAAA+CGG	0.399163	4:+71727621	None:intergenic
TTTGGTGGGTATGGTGAAGG+TGG	0.399953	4:+71728017	None:intergenic
CGAGCTCTTGGAGAAGTTGT+TGG	0.400380	4:+71727438	None:intergenic
CGGGTATGGGTTACCTGGTT+CGG	0.411608	4:+71727980	None:intergenic
ACCGTCTCTGGTGGTGGAAA+TGG	0.419205	4:-71727606	MsG0480022375.01.T01:CDS
TGGGTTACCTGGTTCGGATC+TGG	0.427861	4:+71727986	None:intergenic
GAAGGACCCAAACGAGCTCT+TGG	0.428735	4:+71727426	None:intergenic
TTTAACAGAACCACCGTCTC+TGG	0.436120	4:-71727618	MsG0480022375.01.T01:CDS
CCTGGTTCGGATCTGGTGAC+TGG	0.436545	4:+71727993	None:intergenic
GAAGAGAAAACAGGAAGTAA+TGG	0.438929	4:+71727756	None:intergenic
TTGGTGGGTATGGTGAAGGT+GGG	0.443184	4:+71728018	None:intergenic
TGTTGTAGTTTCAGTGGATC+CGG	0.446853	4:+71727902	None:intergenic
ACAACTTGTTTGAATGAAGA+TGG	0.460677	4:+71727936	None:intergenic
GAGTCTCTTGGAGTAGTTGA+AGG	0.463081	4:+71727489	None:intergenic
CGAGGTTGTTCAGAGTCTCT+TGG	0.465134	4:+71727477	None:intergenic
CCCTGTTACACCTCTTATAC+CGG	0.472248	4:-71727646	MsG0480022375.01.T01:CDS
GAACAAATGTTGTCGGGTAT+GGG	0.473429	4:+71727967	None:intergenic
CTTGGAGTAGTTGAAGGTGA+TGG	0.481947	4:+71727495	None:intergenic
AGACGGTGGTTCTGTTAAAC+GGG	0.486766	4:+71727622	None:intergenic
ACTTCTCCAAGAGCTCGTTT+GGG	0.491521	4:-71727432	MsG0480022375.01.T01:CDS
AATGGTCACCGATGTGGAAA+TGG	0.497928	4:-71727588	MsG0480022375.01.T01:CDS
GCAGGAAAATCTAGAATACT+AGG	0.513283	4:+71727681	None:intergenic
CAGCTTGAACAAATGTTGTC+GGG	0.515878	4:+71727961	None:intergenic
TCAGCTTGAACAAATGTTGT+CGG	0.522642	4:+71727960	None:intergenic
GTGGTTCTGTTAAACGGGTC+CGG	0.531977	4:+71727627	None:intergenic
TGGGTGGAATATGGTTTCGC+AGG	0.534061	4:+71727838	None:intergenic
TTAAACGGGTCCGGTATAAG+AGG	0.542387	4:+71727636	None:intergenic
AGAACCACCGTCTCTGGTGG+TGG	0.543940	4:-71727612	MsG0480022375.01.T01:CDS
GTTGTCGGGTATGGGTTACC+TGG	0.549908	4:+71727975	None:intergenic
GTACTAGTTGAAGAAGAAGA+AGG	0.551766	4:+71727408	None:intergenic
TTGGAGTAGTTGAAGGTGAT+GGG	0.561575	4:+71727496	None:intergenic
CAACTTGTTTGAATGAAGAT+GGG	0.585494	4:+71727937	None:intergenic
ACAGGGCTAAGAACAAGTGC+AGG	0.591317	4:+71727663	None:intergenic
ACCATTTCCACCACCAGAGA+CGG	0.591592	4:+71727605	None:intergenic
GGAGAAGTTGTTGGAAACAG+AGG	0.603466	4:+71727447	None:intergenic
CTGTGGCTATAGCGTGGTGA+AGG	0.608162	4:+71728092	None:intergenic
AGCAACAGTAACAACAACAA+TGG	0.610112	4:-71728056	MsG0480022375.01.T01:CDS
CCAGTCACCAGATCCGAACC+AGG	0.610576	4:-71727993	MsG0480022375.01.T01:CDS
TAGGACTTCCATTTCCACAT+CGG	0.626816	4:+71727580	None:intergenic
CCGGTATAAGAGGTGTAACA+GGG	0.647384	4:+71727646	None:intergenic
AACAGAACCACCGTCTCTGG+TGG	0.652356	4:-71727615	MsG0480022375.01.T01:CDS
GGTGGAAATGGTCACCGATG+TGG	0.656550	4:-71727594	MsG0480022375.01.T01:CDS
ATTTCCACCACCAGAGACGG+TGG	0.659247	4:+71727608	None:intergenic
GGAAACAGAGGAAGCAAACG+AGG	0.664421	4:+71727459	None:intergenic
TGCTACTGCTACATGAACTG+TGG	0.713058	4:+71728075	None:intergenic
CATGAACTGTGGCTATAGCG+TGG	0.720433	4:+71728086	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAGCTATAAGAGAAAAA+GGG	-	Chr4:71728017-71728036	MsG0480022375.01.T01:CDS	20.0%
!	GAAAAAGCTATAAGAGAAAA+AGG	-	Chr4:71728016-71728035	MsG0480022375.01.T01:CDS	25.0%
!	TTTGAATTTGAAGAGAAAAC+AGG	+	Chr4:71727803-71727822	None:intergenic	25.0%
!!!	AGTTTGAAACCAGATTTTTT+GGG	+	Chr4:71727731-71727750	None:intergenic	25.0%
	AAGAGAAAACAGGAAGTAAT+GGG	+	Chr4:71727793-71727812	None:intergenic	30.0%
	ATACTAGGAGACAAAATTTC+AGG	+	Chr4:71727854-71727873	None:intergenic	30.0%
	CAAGTTGTTCAAATGTTAAC+CGG	-	Chr4:71727626-71727645	MsG0480022375.01.T01:CDS	30.0%
	TTCTAGGTGAAAAACTTGAA+GGG	+	Chr4:71727829-71727848	None:intergenic	30.0%
	TTTCTAGGTGAAAAACTTGA+AGG	+	Chr4:71727830-71727849	None:intergenic	30.0%
!	ACAACTTGTTTGAATGAAGA+TGG	+	Chr4:71727614-71727633	None:intergenic	30.0%
!	CAACTTGTTTGAATGAAGAT+GGG	+	Chr4:71727613-71727632	None:intergenic	30.0%
!	TCAATGTTCAAAACTGGTTT+AGG	+	Chr4:71727989-71728008	None:intergenic	30.0%
!!	TTGTTTTGTTGTAGTTTCAG+TGG	+	Chr4:71727654-71727673	None:intergenic	30.0%
!!!	GACAAAATTTCAGGTTTTCT+AGG	+	Chr4:71727845-71727864	None:intergenic	30.0%
!!!	GAGTTTGAAACCAGATTTTT+TGG	+	Chr4:71727732-71727751	None:intergenic	30.0%
	AGCAACAGTAACAACAACAA+TGG	-	Chr4:71727491-71727510	MsG0480022375.01.T01:CDS	35.0%
	CATATTCCACCCAAAAAATC+TGG	-	Chr4:71727719-71727738	MsG0480022375.01.T01:CDS	35.0%
	GAAGAGAAAACAGGAAGTAA+TGG	+	Chr4:71727794-71727813	None:intergenic	35.0%
	GTACTAGTTGAAGAAGAAGA+AGG	+	Chr4:71728142-71728161	None:intergenic	35.0%
	TCAGCTTCAATGTTCAAAAC+TGG	+	Chr4:71727995-71728014	None:intergenic	35.0%
	TCAGCTTGAACAAATGTTGT+CGG	+	Chr4:71727590-71727609	None:intergenic	35.0%
!	GCAGGAAAATCTAGAATACT+AGG	+	Chr4:71727869-71727888	None:intergenic	35.0%
!!	AAACAGGAAGTAATGGGTTT+AGG	+	Chr4:71727787-71727806	None:intergenic	35.0%
!!	GTTGTTGTTGTTGTTGTTTG+AGG	+	Chr4:71727690-71727709	None:intergenic	35.0%
!!!	CAGATTTTTTGGGTGGAATA+TGG	+	Chr4:71727721-71727740	None:intergenic	35.0%
!!!	TTGAAACCAGATTTTTTGGG+TGG	+	Chr4:71727728-71727747	None:intergenic	35.0%
	CAGCTTGAACAAATGTTGTC+GGG	+	Chr4:71727589-71727608	None:intergenic	40.0%
	GAACAAATGTTGTCGGGTAT+GGG	+	Chr4:71727583-71727602	None:intergenic	40.0%
	TAGGACTTCCATTTCCACAT+CGG	+	Chr4:71727970-71727989	None:intergenic	40.0%
	TGAACAAATGTTGTCGGGTA+TGG	+	Chr4:71727584-71727603	None:intergenic	40.0%
	TGTTGTAGTTTCAGTGGATC+CGG	+	Chr4:71727648-71727667	None:intergenic	40.0%
	TTGGAGTAGTTGAAGGTGAT+GGG	+	Chr4:71728054-71728073	None:intergenic	40.0%
!!	GGAAGAGAAGTGTTGTTTCT+TGG	+	Chr4:71727437-71727456	None:intergenic	40.0%
	AACTTCTCCAAGAGCTCGTT+TGG	-	Chr4:71728114-71728133	MsG0480022375.01.T01:CDS	45.0%
	AATGGTCACCGATGTGGAAA+TGG	-	Chr4:71727959-71727978	MsG0480022375.01.T01:CDS	45.0%
	ACTTCTCCAAGAGCTCGTTT+GGG	-	Chr4:71728115-71728134	MsG0480022375.01.T01:CDS	45.0%
	CCCTGTTACACCTCTTATAC+CGG	-	Chr4:71727901-71727920	MsG0480022375.01.T01:CDS	45.0%
	CTTGGAGTAGTTGAAGGTGA+TGG	+	Chr4:71728055-71728074	None:intergenic	45.0%
	GAGTCTCTTGGAGTAGTTGA+AGG	+	Chr4:71728061-71728080	None:intergenic	45.0%
	GGAGAAGTTGTTGGAAACAG+AGG	+	Chr4:71728103-71728122	None:intergenic	45.0%
	TGCTACTGCTACATGAACTG+TGG	+	Chr4:71727475-71727494	None:intergenic	45.0%
	TTAAACGGGTCCGGTATAAG+AGG	+	Chr4:71727914-71727933	None:intergenic	45.0%
	TTTAACAGAACCACCGTCTC+TGG	-	Chr4:71727929-71727948	MsG0480022375.01.T01:CDS	45.0%
!	AGACGGTGGTTCTGTTAAAC+GGG	+	Chr4:71727928-71727947	None:intergenic	45.0%
!	CCGGTATAAGAGGTGTAACA+GGG	+	Chr4:71727904-71727923	None:intergenic	45.0%
!	GAGACGGTGGTTCTGTTAAA+CGG	+	Chr4:71727929-71727948	None:intergenic	45.0%
!	TCCGGTATAAGAGGTGTAAC+AGG	+	Chr4:71727905-71727924	None:intergenic	45.0%
!!!	ATCTGGTGACTGGTTTTGGT+GGG	+	Chr4:71727547-71727566	None:intergenic	45.0%
	ACAGGGCTAAGAACAAGTGC+AGG	+	Chr4:71727887-71727906	None:intergenic	50.0%
	ACCATTTCCACCACCAGAGA+CGG	+	Chr4:71727945-71727964	None:intergenic	50.0%
	CATGAACTGTGGCTATAGCG+TGG	+	Chr4:71727464-71727483	None:intergenic	50.0%
	CGAGCTCTTGGAGAAGTTGT+TGG	+	Chr4:71728112-71728131	None:intergenic	50.0%
	CGAGGTTGTTCAGAGTCTCT+TGG	+	Chr4:71728073-71728092	None:intergenic	50.0%
	GGAAACAGAGGAAGCAAACG+AGG	+	Chr4:71728091-71728110	None:intergenic	50.0%
	TGGGTGGAATATGGTTTCGC+AGG	+	Chr4:71727712-71727731	None:intergenic	50.0%
!	GTGGTTCTGTTAAACGGGTC+CGG	+	Chr4:71727923-71727942	None:intergenic	50.0%
!	TTGGTGGGTATGGTGAAGGT+GGG	+	Chr4:71727532-71727551	None:intergenic	50.0%
!	TTTGGTGGGTATGGTGAAGG+TGG	+	Chr4:71727533-71727552	None:intergenic	50.0%
!!	GGTTTTGGTGGGTATGGTGA+AGG	+	Chr4:71727536-71727555	None:intergenic	50.0%
!!!	GATCTGGTGACTGGTTTTGG+TGG	+	Chr4:71727548-71727567	None:intergenic	50.0%
!!!	GTGACTGGTTTTGGTGGGTA+TGG	+	Chr4:71727542-71727561	None:intergenic	50.0%
!!!	TCGGATCTGGTGACTGGTTT+TGG	+	Chr4:71727551-71727570	None:intergenic	50.0%
	AACAGAACCACCGTCTCTGG+TGG	-	Chr4:71727932-71727951	MsG0480022375.01.T01:CDS	55.0%
	ATTTCCACCACCAGAGACGG+TGG	+	Chr4:71727942-71727961	None:intergenic	55.0%
	CTGTGGCTATAGCGTGGTGA+AGG	+	Chr4:71727458-71727477	None:intergenic	55.0%
	GAAGGACCCAAACGAGCTCT+TGG	+	Chr4:71728124-71728143	None:intergenic	55.0%
	GGTGGAAATGGTCACCGATG+TGG	-	Chr4:71727953-71727972	MsG0480022375.01.T01:CDS	55.0%
!	CGGGTATGGGTTACCTGGTT+CGG	+	Chr4:71727570-71727589	None:intergenic	55.0%
!	GTTGTCGGGTATGGGTTACC+TGG	+	Chr4:71727575-71727594	None:intergenic	55.0%
!!	ACCGTCTCTGGTGGTGGAAA+TGG	-	Chr4:71727941-71727960	MsG0480022375.01.T01:CDS	55.0%
!!	TGGGTTACCTGGTTCGGATC+TGG	+	Chr4:71727564-71727583	None:intergenic	55.0%
	AGAACCACCGTCTCTGGTGG+TGG	-	Chr4:71727935-71727954	MsG0480022375.01.T01:CDS	60.0%
	CCAGTCACCAGATCCGAACC+AGG	-	Chr4:71727554-71727573	MsG0480022375.01.T01:CDS	60.0%
!!	CCTGGTTCGGATCTGGTGAC+TGG	+	Chr4:71727557-71727576	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	71727404	71728165	71727404	ID=MsG0480022375.01;Name=MsG0480022375.01
Chr4	mRNA	71727404	71728165	71727404	ID=MsG0480022375.01.T01;Parent=MsG0480022375.01;Name=MsG0480022375.01.T01;_AED=0.26;_eAED=0.26;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|253
Chr4	exon	71727404	71728165	71727404	ID=MsG0480022375.01.T01:exon:10005;Parent=MsG0480022375.01.T01
Chr4	CDS	71727404	71728165	71727404	ID=MsG0480022375.01.T01:cds;Parent=MsG0480022375.01.T01

Gene Sequence

>MsG0480022375.01.T01

ATGGAGTCAAACAACAACAACATGAAATCACCAAGAAACAACACTTCTCTTCCTTCACCACGCTATAGCCACAGTTCATGTAGCAGTAGCAACAGTAACAACAACAATGGAACAATTCATCATAACCCACCTTCACCATACCCACCAAAACCAGTCACCAGATCCGAACCAGGTAACCCATACCCGACAACATTTGTTCAAGCTGACCCATCTTCATTCAAACAAGTTGTTCAAATGTTAACCGGATCCACTGAAACTACAACAAAACAAACATCATCAATTTCCTCAAACAACAACAACAACAACCTGCGAAACCATATTCCACCCAAAAAATCTGGTTTCAAACTCTATGAACGAAGAAACTCTCTGAAAAATCTTCACCTAAACCCATTACTTCCTGTTTTCTCTTCAAATTCAAACAACCCTTCAAGTTTTTCACCTAGAAAACCTGAAATTTTGTCTCCTAGTATTCTAGATTTTCCTGCACTTGTTCTTAGCCCTGTTACACCTCTTATACCGGACCCGTTTAACAGAACCACCGTCTCTGGTGGTGGAAATGGTCACCGATGTGGAAATGGAAGTCCTAAACCAGTTTTGAACATTGAAGCTGAAGAAAAAGCTATAAGAGAAAAAGGGTTTTTCTTACACCCATCACCTTCAACTACTCCAAGAGACTCTGAACAACCTCGTTTGCTTCCTCTGTTTCCAACAACTTCTCCAAGAGCTCGTTTGGGTCCTTCTTCTTCTTCAACTAGTACTTGA

Protein sequence

>MsG0480022375.01.T01

MESNNNNMKSPRNNTSLPSPRYSHSSCSSSNSNNNNGTIHHNPPSPYPPKPVTRSEPGNPYPTTFVQADPSSFKQVVQMLTGSTETTTKQTSSISSNNNNNNLRNHIPPKKSGFKLYERRNSLKNLHLNPLLPVFSSNSNNPSSFSPRKPEILSPSILDFPALVLSPVTPLIPDPFNRTTVSGGGNGHRCGNGSPKPVLNIEAEEKAIREKGFFLHPSPSTTPRDSEQPRLLPLFPTTSPRARLGPSSSSTST*