AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022694.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022694.01.T01 MTR_4g094315 75.873 315 52 5 1 306 1 300 1.01e-161 459
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022694.01.T01 AT3G26410 57.143 308 117 4 1 306 1 295 2.29e-113 336

Find 90 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAATCTAACAGCACTTTC+AGG 0.261458 4:-75566110 MsG0480022694.01.T01:CDS
ATCAAAAGCTCCAAACAATT+TGG 0.282090 4:+75566621 None:intergenic
TGGGATGAACGTCGTCGTTT+TGG 0.299965 4:+75566593 None:intergenic
AGGATTACTGTTGATACCTT+TGG 0.304749 4:-75566090 MsG0480022694.01.T01:CDS
CTCTTTCAGCCCCGGACGCC+AGG 0.306574 4:+75564223 None:intergenic
ACCAAATGGAAAGGAGAATC+TGG 0.336337 4:+75566539 None:intergenic
TGAAAAGCCCGTACTTATCT+TGG 0.337225 4:-75564838 MsG0480022694.01.T01:CDS
AACCTTCCTCCCTGGCGTCC+GGG 0.349511 4:-75564233 MsG0480022694.01.T01:CDS
CAACCTTCCTCCCTGGCGTC+CGG 0.354356 4:-75564234 MsG0480022694.01.T01:CDS
CCCAGATTCTCCTTTCCATT+TGG 0.355570 4:-75566540 MsG0480022694.01.T01:CDS
GAAAACCAGAGCACAACTTC+TGG 0.364397 4:-75564979 MsG0480022694.01.T01:CDS
TTTGTTGCAGCAGCTCATTT+TGG 0.370684 4:-75564713 MsG0480022694.01.T01:CDS
GAATCTTTAGCCAAATTGTT+TGG 0.372629 4:-75566631 MsG0480022694.01.T01:CDS
AGGGTGCTGACATTGACATT+AGG 0.374088 4:-75564522 MsG0480022694.01.T01:CDS
TATGGCAACTTTCTCTCATC+TGG 0.376539 4:+75566135 None:intergenic
GGGTGCTGACATTGACATTA+GGG 0.379288 4:-75564521 MsG0480022694.01.T01:CDS
TAAAATTACTCCAAACATTA+CGG 0.379933 4:+75564473 None:intergenic
GTGCTGTTAGATTCCAAGTA+TGG 0.387116 4:+75566117 None:intergenic
GGATTACTGTTGATACCTTT+GGG 0.387647 4:-75566089 MsG0480022694.01.T01:CDS
GATAATTGTGAAGGTAATAA+TGG 0.389973 4:-75564944 MsG0480022694.01.T01:CDS
GAGGGAGCTTCCATTGCAAC+TGG 0.392144 4:+75566573 None:intergenic
TGTATGAGCTATGGGGTGAA+GGG 0.410913 4:-75566197 MsG0480022694.01.T01:CDS
AACCAAGCACTGGCTACTCC+GGG 0.421705 4:-75564775 MsG0480022694.01.T01:CDS
CAACCAAGCACTGGCTACTC+CGG 0.425995 4:-75564776 MsG0480022694.01.T01:CDS
GCGGTCCTGACCGTAATGTT+TGG 0.431493 4:-75564483 MsG0480022694.01.T01:CDS
ATGTATGAGCTATGGGGTGA+AGG 0.439043 4:-75566198 MsG0480022694.01.T01:CDS
GTTTACTTATATCCCTTTCA+AGG 0.440303 4:-75566025 MsG0480022694.01.T01:intron
ACTAGTTTATGACCCTTTGT+TGG 0.442233 4:-75564747 MsG0480022694.01.T01:CDS
TTATGACCCTTTGTTGGAAC+CGG 0.442851 4:-75564741 MsG0480022694.01.T01:CDS
TGAGAGAAAGTTGCCATACT+TGG 0.448763 4:-75566130 MsG0480022694.01.T01:CDS
CTGAAGGGAGATTGACCAAA+TGG 0.448856 4:+75566525 None:intergenic
GAATGAGTTCCTTATGCTCC+TGG 0.450036 4:+75566052 None:intergenic
GTGAAGTTGGTGCTGCTGAT+AGG 0.451208 4:-75564883 MsG0480022694.01.T01:CDS
GGGTAGCTATGAGGAGTTGA+AGG 0.451262 4:-75566175 MsG0480022694.01.T01:CDS
ATCAGCCAGAAGTTGTGCTC+TGG 0.451410 4:+75564974 None:intergenic
TGGGAAGGTTATTAGTCTCC+AGG 0.454971 4:-75566070 MsG0480022694.01.T01:CDS
GAAAAGCCCGTACTTATCTT+GGG 0.468843 4:-75564837 MsG0480022694.01.T01:CDS
ACCTTCCTCCCTGGCGTCCG+GGG 0.473468 4:-75564232 MsG0480022694.01.T01:CDS
TATTAGTCTCCAGGAGCATA+AGG 0.475181 4:-75566061 MsG0480022694.01.T01:CDS
AAGGAGAATCTGGGTGATGA+TGG 0.479452 4:+75566549 None:intergenic
TAAAGGACACACACCTTGAA+AGG 0.487952 4:+75566012 None:intergenic
GCTGCTGCAACAAATACTTC+CGG 0.493463 4:+75564722 None:intergenic
TCAGCCCTTAAAAGACCAAC+TGG 0.494342 4:+75564260 None:intergenic
CTGGCGTCCGGGGCTGAAAG+AGG 0.505882 4:-75564222 MsG0480022694.01.T01:CDS
TTACGAGCAATTTCTTCTGA+AGG 0.511567 4:+75566509 None:intergenic
CGGAGTAGCCAGTGCTTGGT+TGG 0.514225 4:+75564777 None:intergenic
GCCCCGGACGCCAGGGAGGA+AGG 0.514958 4:+75564231 None:intergenic
TCTAATGGCCAACCAAGCAC+TGG 0.516528 4:-75564785 MsG0480022694.01.T01:CDS
GAGCATAAGGAACTCATTCA+AGG 0.518919 4:-75566048 MsG0480022694.01.T01:CDS
TACTGTTGATACCTTTGGGA+AGG 0.526326 4:-75566085 MsG0480022694.01.T01:CDS
ATACTTCCGGTTCCAACAAA+GGG 0.528270 4:+75564735 None:intergenic
GACGTTCATCCCAGTTGCAA+TGG 0.529598 4:-75566583 MsG0480022694.01.T01:CDS
TACGAGCAATTTCTTCTGAA+GGG 0.530624 4:+75566510 None:intergenic
TCTTTCAGCCCCGGACGCCA+GGG 0.533800 4:+75564224 None:intergenic
GAAGGGAATGTATGAGCTAT+GGG 0.534827 4:-75566205 MsG0480022694.01.T01:CDS
TTATCTTGGGCCAACAGCCA+TGG 0.536034 4:-75564824 MsG0480022694.01.T01:CDS
ATCTCCGCATCCATGGCTGT+TGG 0.537799 4:+75564814 None:intergenic
GTATATGTTGCAGTATGGAT+TGG 0.538931 4:-75564288 MsG0480022694.01.T01:intron
AAAGGACACACACCTTGAAA+GGG 0.544820 4:+75566013 None:intergenic
GAATCTGGGTGATGATGGAG+AGG 0.548209 4:+75566554 None:intergenic
AAATTTACCTCTTTCAGCCC+CGG 0.552977 4:+75564215 None:intergenic
TGAAGGGAATGTATGAGCTA+TGG 0.553852 4:-75566206 MsG0480022694.01.T01:CDS
TTATGTAGGTATACTTGTGA+AGG 0.555230 4:-75566223 MsG0480022694.01.T01:intron
CCAAGCACTGGCTACTCCGG+GGG 0.555832 4:-75564773 MsG0480022694.01.T01:CDS
GTATGAGCTATGGGGTGAAG+GGG 0.562156 4:-75566196 MsG0480022694.01.T01:CDS
CTGACAACAACCTTCCTCCC+TGG 0.564474 4:-75564241 MsG0480022694.01.T01:CDS
TTACTCCAAACATTACGGTC+AGG 0.565054 4:+75564478 None:intergenic
TGTTGGCCCAAGATAAGTAC+GGG 0.569595 4:+75564831 None:intergenic
AATACTTCCGGTTCCAACAA+AGG 0.571600 4:+75564734 None:intergenic
AAAAGCTATCTCCGCATCCA+TGG 0.574264 4:+75564807 None:intergenic
TGGGCCAACAGCCATGGATG+CGG 0.579242 4:-75564818 MsG0480022694.01.T01:CDS
TATGAGCTATGGGGTGAAGG+GGG 0.582219 4:-75566195 MsG0480022694.01.T01:CDS
CCAAATGGAAAGGAGAATCT+GGG 0.583278 4:+75566540 None:intergenic
CTGTTGGCCCAAGATAAGTA+CGG 0.584766 4:+75564830 None:intergenic
ATAGAAATTGATAATTGTGA+AGG 0.588940 4:-75564953 MsG0480022694.01.T01:CDS
AGCATAAGGAACTCATTCAA+GGG 0.598978 4:-75566047 MsG0480022694.01.T01:CDS
TTTGGCTAAAGATTCGACTT+CGG 0.604259 4:+75566639 None:intergenic
TTCAGCCCCGGACGCCAGGG+AGG 0.619819 4:+75564227 None:intergenic
TATGTAGGTATACTTGTGAA+GGG 0.620517 4:-75566222 MsG0480022694.01.T01:intron
GACATTAGGGTAGTACGTGA+TGG 0.622739 4:-75564508 MsG0480022694.01.T01:CDS
GGGAGATTGACCAAATGGAA+AGG 0.626339 4:+75566530 None:intergenic
ACCAAGCACTGGCTACTCCG+GGG 0.632251 4:-75564774 MsG0480022694.01.T01:CDS
AGGGAGCTTCCATTGCAACT+GGG 0.635926 4:+75566574 None:intergenic
AGGGTAGTACGTGATGGACG+CGG 0.642357 4:-75564502 MsG0480022694.01.T01:CDS
TATGGATTGGAAATGCCAGT+TGG 0.653758 4:-75564275 MsG0480022694.01.T01:CDS
GAGACTAATAACCTTCCCAA+AGG 0.682399 4:+75566074 None:intergenic
CAAGCACTGGCTACTCCGGG+GGG 0.693232 4:-75564772 MsG0480022694.01.T01:CDS
AATCTGGGTGATGATGGAGA+GGG 0.697131 4:+75566555 None:intergenic
GAAAACACATAGATACCACA+TGG 0.722516 4:+75566690 None:intergenic
AAGGGAATGTATGAGCTATG+GGG 0.749415 4:-75566204 MsG0480022694.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATGACAAATAATTAAA+GGG + Chr4:75564490-75564509 None:intergenic 10.0%
!! CAATAAATAAATCAAATTAA+AGG + Chr4:75564907-75564926 None:intergenic 10.0%
!! TAAAAATGACAAATAATTAA+AGG + Chr4:75564491-75564510 None:intergenic 10.0%
!!! AAATTTTATTTTTTTATGTG+GGG - Chr4:75564647-75564666 MsG0480022694.01.T01:intron 10.0%
!!! AAGTTTTAATTTTTTATAGT+GGG - Chr4:75564615-75564634 MsG0480022694.01.T01:intron 10.0%
!!! AATTATTTGTCATTTTTATT+TGG - Chr4:75564493-75564512 MsG0480022694.01.T01:CDS 10.0%
!!! ATTATTTGTCATTTTTATTT+GGG - Chr4:75564494-75564513 MsG0480022694.01.T01:CDS 10.0%
!!! AAAGTTTCAATTTTCATAAT+GGG - Chr4:75564555-75564574 MsG0480022694.01.T01:intron 15.0%
!!! AAATTTTTGATTTTTTTACG+TGG - Chr4:75564524-75564543 MsG0480022694.01.T01:intron 15.0%
!!! AAGTTTATCATTTTTATAGT+GGG - Chr4:75564585-75564604 MsG0480022694.01.T01:intron 15.0%
!!! AATTTTTGATTTTTTTACGT+GGG - Chr4:75564525-75564544 MsG0480022694.01.T01:intron 15.0%
!!! ATTTGATTTATTTATTGCAT+TGG - Chr4:75564910-75564929 MsG0480022694.01.T01:CDS 15.0%
!!! ATTTTTTCAATTAAGATAAC+AGG - Chr4:75565382-75565401 MsG0480022694.01.T01:intron 15.0%
!!! GAAGTTTTAATTTTTTATAG+TGG - Chr4:75564614-75564633 MsG0480022694.01.T01:intron 15.0%
!!! TAAAGTTTCAATTTTCATAA+TGG - Chr4:75564554-75564573 MsG0480022694.01.T01:intron 15.0%
!!! TAAGTTTATCATTTTTATAG+TGG - Chr4:75564584-75564603 MsG0480022694.01.T01:intron 15.0%
!!! TTTTTTCAATTAAGATAACA+GGG - Chr4:75565383-75565402 MsG0480022694.01.T01:intron 15.0%
!! AGAAAGAAAAAGAAAAGAAA+AGG + Chr4:75566251-75566270 None:intergenic 20.0%
!! ATACTTAATCTAAATTGTTC+AGG - Chr4:75566357-75566376 MsG0480022694.01.T01:intron 20.0%
!! TAAAATTACTCCAAACATTA+CGG + Chr4:75566429-75566448 None:intergenic 20.0%
!! TACTTAATCTAAATTGTTCA+GGG - Chr4:75566358-75566377 MsG0480022694.01.T01:intron 20.0%
!! TATCTTTCTGATAAACTTAT+TGG - Chr4:75566586-75566605 MsG0480022694.01.T01:CDS 20.0%
!!! AAAAAAGTACAATCAAACAA+GGG + Chr4:75565644-75565663 None:intergenic 20.0%
!!! ATAGAAATTGATAATTGTGA+AGG - Chr4:75565946-75565965 MsG0480022694.01.T01:intron 20.0%
!!! ATGTTTTTACACTTGTTTTA+TGG - Chr4:75566467-75566486 MsG0480022694.01.T01:intron 20.0%
!!! TTTTTCAATTAAGATAACAG+GGG - Chr4:75565384-75565403 MsG0480022694.01.T01:intron 20.0%
! GATAATTGTGAAGGTAATAA+TGG - Chr4:75565955-75565974 MsG0480022694.01.T01:intron 25.0%
! GCATGATTAAAACTACATTA+TGG - Chr4:75565185-75565204 MsG0480022694.01.T01:intron 25.0%
!! AAAATTCTCTTTTGTTCAAC+TGG + Chr4:75565988-75566007 None:intergenic 25.0%
!! CAAAAAAGTACAATCAAACA+AGG + Chr4:75565645-75565664 None:intergenic 25.0%
!! CAAGTTGAATTTGTAATGTT+GGG - Chr4:75565291-75565310 MsG0480022694.01.T01:intron 25.0%
!! CATCTGAAATGAAAACATAT+GGG + Chr4:75565773-75565792 None:intergenic 25.0%
!! GAACAAAAGAGAATTTTCTT+TGG - Chr4:75565991-75566010 MsG0480022694.01.T01:intron 25.0%
!!! ATGCTTACTTTTGATATATG+TGG - Chr4:75565063-75565082 MsG0480022694.01.T01:intron 25.0%
!!! CTATCAATTGCTATTTACAT+AGG + Chr4:75564984-75565003 None:intergenic 25.0%
!!! TTTTATTTTTTTATGTGGGG+TGG - Chr4:75564650-75564669 MsG0480022694.01.T01:intron 25.0%
AAAATTAACCTGCAAAATCC+TGG + Chr4:75565675-75565694 None:intergenic 30.0%
AGTCTACTACATCATATAAG+TGG + Chr4:75566530-75566549 None:intergenic 30.0%
ATCAAAAGCTCCAAACAATT+TGG + Chr4:75564281-75564300 None:intergenic 30.0%
GAAACTGTATCATCTGATTT+GGG - Chr4:75565158-75565177 MsG0480022694.01.T01:intron 30.0%
GAATCTTTAGCCAAATTGTT+TGG - Chr4:75564268-75564287 MsG0480022694.01.T01:CDS 30.0%
GACAAAAGAAAGATACTTGT+TGG + Chr4:75565856-75565875 None:intergenic 30.0%
GTTTACTTATATCCCTTTCA+AGG - Chr4:75564874-75564893 MsG0480022694.01.T01:CDS 30.0%
TATGTAGGTATACTTGTGAA+GGG - Chr4:75564677-75564696 MsG0480022694.01.T01:intron 30.0%
TCATACAAGCAAAATAGATC+TGG + Chr4:75565520-75565539 None:intergenic 30.0%
TTATGTAGGTATACTTGTGA+AGG - Chr4:75564676-75564695 MsG0480022694.01.T01:intron 30.0%
TTTGATATATGTGGTGCAAA+GGG - Chr4:75565072-75565091 MsG0480022694.01.T01:intron 30.0%
! AACTACATTATGGTTCTGTT+CGG - Chr4:75565195-75565214 MsG0480022694.01.T01:intron 30.0%
! AATTTTGTCTTGCTAAACAG+AGG - Chr4:75565575-75565594 MsG0480022694.01.T01:intron 30.0%
! ATCTATGTGTTTTCTTTCAC+AGG - Chr4:75564218-75564237 MsG0480022694.01.T01:CDS 30.0%
! GCAAGTTGAATTTGTAATGT+TGG - Chr4:75565290-75565309 MsG0480022694.01.T01:intron 30.0%
! GCATCTGAAATGAAAACATA+TGG + Chr4:75565774-75565793 None:intergenic 30.0%
! TATTGCAATGGATTCTGTTT+AGG - Chr4:75566294-75566313 MsG0480022694.01.T01:intron 30.0%
! TCTATTCACTGTCATTTCAT+TGG - Chr4:75565136-75565155 MsG0480022694.01.T01:intron 30.0%
! TGTTTTCTTTCACAGGTTAT+TGG - Chr4:75564225-75564244 MsG0480022694.01.T01:CDS 30.0%
! TTCTTTTGTCCATCATTGTT+TGG - Chr4:75565866-75565885 MsG0480022694.01.T01:intron 30.0%
! TTTTGATATATGTGGTGCAA+AGG - Chr4:75565071-75565090 MsG0480022694.01.T01:intron 30.0%
!! GGAGTATGAGTTTTTCAAAA+AGG + Chr4:75565835-75565854 None:intergenic 30.0%
!! TGTTTGGAGTAATTTTAAGC+AGG - Chr4:75566432-75566451 MsG0480022694.01.T01:intron 30.0%
!!! GTACTTTTTTGTTAGTATCC+AGG - Chr4:75565654-75565673 MsG0480022694.01.T01:intron 30.0%
ACAAAGTGACCAAACAATGA+TGG + Chr4:75565878-75565897 None:intergenic 35.0%
AGCATAAGGAACTCATTCAA+GGG - Chr4:75564852-75564871 MsG0480022694.01.T01:CDS 35.0%
AGGATTACTGTTGATACCTT+TGG - Chr4:75564809-75564828 MsG0480022694.01.T01:CDS 35.0%
AGTACAATCAAACAAGGGAT+AGG + Chr4:75565639-75565658 None:intergenic 35.0%
ATAACAGGGGTAAAATTGAG+AGG - Chr4:75565397-75565416 MsG0480022694.01.T01:intron 35.0%
CTTACTCACTATCATCTACA+AGG + Chr4:75565267-75565286 None:intergenic 35.0%
GAAAAGGTGAAGAATGAAGA+AGG + Chr4:75566235-75566254 None:intergenic 35.0%
GGAAACTGTATCATCTGATT+TGG - Chr4:75565157-75565176 MsG0480022694.01.T01:intron 35.0%
GGATAGGAAACACATAATTC+CGG + Chr4:75565623-75565642 None:intergenic 35.0%
GGATTACTGTTGATACCTTT+GGG - Chr4:75564810-75564829 MsG0480022694.01.T01:CDS 35.0%
GTATATGTTGCAGTATGGAT+TGG - Chr4:75566611-75566630 MsG0480022694.01.T01:CDS 35.0%
TAACAGGGGTAAAATTGAGA+GGG - Chr4:75565398-75565417 MsG0480022694.01.T01:intron 35.0%
TACGAGCAATTTCTTCTGAA+GGG + Chr4:75564392-75564411 None:intergenic 35.0%
TAGATCGTGAAAGGATAAGT+TGG + Chr4:75566276-75566295 None:intergenic 35.0%
TTACGAGCAATTTCTTCTGA+AGG + Chr4:75564393-75564412 None:intergenic 35.0%
TTTGGCTAAAGATTCGACTT+CGG + Chr4:75564263-75564282 None:intergenic 35.0%
! ACTAGTTTATGACCCTTTGT+TGG - Chr4:75566152-75566171 MsG0480022694.01.T01:CDS 35.0%
! ATTCTCTTTTGTTCAACTGG+AGG + Chr4:75565985-75566004 None:intergenic 35.0%
! ATTTTCTTTGGTCGTGAAGT+TGG - Chr4:75566003-75566022 MsG0480022694.01.T01:intron 35.0%
! GTTGATGAACTAGCTTATAG+CGG - Chr4:75565237-75565256 MsG0480022694.01.T01:intron 35.0%
!! TGTATCATCTGATTTGGGTT+TGG - Chr4:75565163-75565182 MsG0480022694.01.T01:intron 35.0%
!!! AAATGCCAGTTGGTCTTTTA+AGG - Chr4:75566634-75566653 MsG0480022694.01.T01:CDS 35.0%
!!! AATGCCAGTTGGTCTTTTAA+GGG - Chr4:75566635-75566654 MsG0480022694.01.T01:CDS 35.0%
AAAGGACACACACCTTGAAA+GGG + Chr4:75564889-75564908 None:intergenic 40.0%
AAATTTACCTCTTTCAGCCC+CGG + Chr4:75566687-75566706 None:intergenic 40.0%
AAGGGAATGTATGAGCTATG+GGG - Chr4:75564695-75564714 MsG0480022694.01.T01:intron 40.0%
AATACTTCCGGTTCCAACAA+AGG + Chr4:75566168-75566187 None:intergenic 40.0%
ACCAAATGGAAAGGAGAATC+TGG + Chr4:75564363-75564382 None:intergenic 40.0%
ATACTTCCGGTTCCAACAAA+GGG + Chr4:75566167-75566186 None:intergenic 40.0%
CATGGTAAATTATGACCACG+AGG - Chr4:75564942-75564961 MsG0480022694.01.T01:CDS 40.0%
CCAAATGGAAAGGAGAATCT+GGG + Chr4:75564362-75564381 None:intergenic 40.0%
CCATTGCAATAGATCGTGAA+AGG + Chr4:75566285-75566304 None:intergenic 40.0%
CCTTTCACGATCTATTGCAA+TGG - Chr4:75566282-75566301 MsG0480022694.01.T01:intron 40.0%
GAAAAGCCCGTACTTATCTT+GGG - Chr4:75566062-75566081 MsG0480022694.01.T01:CDS 40.0%
GAAGGGAATGTATGAGCTAT+GGG - Chr4:75564694-75564713 MsG0480022694.01.T01:intron 40.0%
GAGACTAATAACCTTCCCAA+AGG + Chr4:75564828-75564847 None:intergenic 40.0%
GAGCATAAGGAACTCATTCA+AGG - Chr4:75564851-75564870 MsG0480022694.01.T01:CDS 40.0%
TAAAGGACACACACCTTGAA+AGG + Chr4:75564890-75564909 None:intergenic 40.0%
TAGCTTCTATTGAGAGTTGC+CGG - Chr4:75565601-75565620 MsG0480022694.01.T01:intron 40.0%
TATGGCAACTTTCTCTCATC+TGG + Chr4:75564767-75564786 None:intergenic 40.0%
TGAAAAGCCCGTACTTATCT+TGG - Chr4:75566061-75566080 MsG0480022694.01.T01:CDS 40.0%
TGAAGGGAATGTATGAGCTA+TGG - Chr4:75564693-75564712 MsG0480022694.01.T01:intron 40.0%
TGAGAGAAAGTTGCCATACT+TGG - Chr4:75564769-75564788 MsG0480022694.01.T01:CDS 40.0%
TGGAATCTAACAGCACTTTC+AGG - Chr4:75564789-75564808 MsG0480022694.01.T01:CDS 40.0%
TGGCTGTATATGTTGCAGTA+TGG - Chr4:75566606-75566625 MsG0480022694.01.T01:CDS 40.0%
TTACTCCAAACATTACGGTC+AGG + Chr4:75566424-75566443 None:intergenic 40.0%
TTATGACCCTTTGTTGGAAC+CGG - Chr4:75566158-75566177 MsG0480022694.01.T01:CDS 40.0%
! GTGCTGTTAGATTCCAAGTA+TGG + Chr4:75564785-75564804 None:intergenic 40.0%
! GTTAGTATCCAGGATTTTGC+AGG - Chr4:75565664-75565683 MsG0480022694.01.T01:intron 40.0%
! GTTTGGTCACTTTGTTTGCA+GGG - Chr4:75565883-75565902 MsG0480022694.01.T01:intron 40.0%
! TACTGTTGATACCTTTGGGA+AGG - Chr4:75564814-75564833 MsG0480022694.01.T01:CDS 40.0%
! TATTAGTCTCCAGGAGCATA+AGG - Chr4:75564838-75564857 MsG0480022694.01.T01:CDS 40.0%
! TGCGGAGATAGCTTTTCTAA+TGG - Chr4:75566099-75566118 MsG0480022694.01.T01:CDS 40.0%
! TGTTTGGTCACTTTGTTTGC+AGG - Chr4:75565882-75565901 MsG0480022694.01.T01:intron 40.0%
! TTTGTTGCAGCAGCTCATTT+TGG - Chr4:75566186-75566205 MsG0480022694.01.T01:CDS 40.0%
!! TATGGATTGGAAATGCCAGT+TGG - Chr4:75566624-75566643 MsG0480022694.01.T01:CDS 40.0%
!! TCAATAGTTTGCTGCACTCA+AGG + Chr4:75565039-75565058 None:intergenic 40.0%
!!! TCATTTTGGAGCAATGACCA+TGG - Chr4:75566200-75566219 MsG0480022694.01.T01:CDS 40.0%
AAAAGCTATCTCCGCATCCA+TGG + Chr4:75566095-75566114 None:intergenic 45.0%
AAGGAGAATCTGGGTGATGA+TGG + Chr4:75564353-75564372 None:intergenic 45.0%
ATGTATGAGCTATGGGGTGA+AGG - Chr4:75564701-75564720 MsG0480022694.01.T01:CDS 45.0%
ATGTGGGGTGGCAATTATGT+AGG - Chr4:75564662-75564681 MsG0480022694.01.T01:intron 45.0%
CCCAGATTCTCCTTTCCATT+TGG - Chr4:75564359-75564378 MsG0480022694.01.T01:intron 45.0%
CTGAAGGGAGATTGACCAAA+TGG + Chr4:75564377-75564396 None:intergenic 45.0%
CTGTTGGCCCAAGATAAGTA+CGG + Chr4:75566072-75566091 None:intergenic 45.0%
GAAAACCAGAGCACAACTTC+TGG - Chr4:75565920-75565939 MsG0480022694.01.T01:intron 45.0%
GAATGAGTTCCTTATGCTCC+TGG + Chr4:75564850-75564869 None:intergenic 45.0%
GACATTAGGGTAGTACGTGA+TGG - Chr4:75566391-75566410 MsG0480022694.01.T01:intron 45.0%
GCTGCTGCAACAAATACTTC+CGG + Chr4:75566180-75566199 None:intergenic 45.0%
GGGAGATTGACCAAATGGAA+AGG + Chr4:75564372-75564391 None:intergenic 45.0%
TCAGCCCTTAAAAGACCAAC+TGG + Chr4:75566642-75566661 None:intergenic 45.0%
TGGGAATAAGAGATGCACAC+AGG + Chr4:75565754-75565773 None:intergenic 45.0%
TGTATGAGCTATGGGGTGAA+GGG - Chr4:75564702-75564721 MsG0480022694.01.T01:CDS 45.0%
TGTTGGCCCAAGATAAGTAC+GGG + Chr4:75566071-75566090 None:intergenic 45.0%
! GGGCTTTTCAACTCGTATGT+CGG + Chr4:75566051-75566070 None:intergenic 45.0%
! GTCATAAACTAGTTTCCCCC+CGG + Chr4:75566145-75566164 None:intergenic 45.0%
! TGGGAAGGTTATTAGTCTCC+AGG - Chr4:75564829-75564848 MsG0480022694.01.T01:CDS 45.0%
!! AATCTGGGTGATGATGGAGA+GGG + Chr4:75564347-75564366 None:intergenic 45.0%
!! AGGGTGCTGACATTGACATT+AGG - Chr4:75566377-75566396 MsG0480022694.01.T01:intron 45.0%
!! GGGTGCTGACATTGACATTA+GGG - Chr4:75566378-75566397 MsG0480022694.01.T01:intron 45.0%
!!! AAAAATTTTATTTTTTTATG+TGG - Chr4:75564645-75564664 MsG0480022694.01.T01:intron 5.0%
!!! AAAATTTTATTTTTTTATGT+GGG - Chr4:75564646-75564665 MsG0480022694.01.T01:intron 5.0%
AGGGAGCTTCCATTGCAACT+GGG + Chr4:75564328-75564347 None:intergenic 50.0%
ATCAGCCAGAAGTTGTGCTC+TGG + Chr4:75565928-75565947 None:intergenic 50.0%
CTGAAGTGACGATAACCTCG+TGG + Chr4:75564960-75564979 None:intergenic 50.0%
GAAGAAGGAGAAGCAGACCA+TGG + Chr4:75566220-75566239 None:intergenic 50.0%
GACGTTCATCCCAGTTGCAA+TGG - Chr4:75564316-75564335 MsG0480022694.01.T01:intron 50.0%
GGGTAGCTATGAGGAGTTGA+AGG - Chr4:75564724-75564743 MsG0480022694.01.T01:CDS 50.0%
GTATGAGCTATGGGGTGAAG+GGG - Chr4:75564703-75564722 MsG0480022694.01.T01:CDS 50.0%
TATGAGCTATGGGGTGAAGG+GGG - Chr4:75564704-75564723 MsG0480022694.01.T01:CDS 50.0%
TCTAATGGCCAACCAAGCAC+TGG - Chr4:75566114-75566133 MsG0480022694.01.T01:CDS 50.0%
TTATCTTGGGCCAACAGCCA+TGG - Chr4:75566075-75566094 MsG0480022694.01.T01:CDS 50.0%
! TGGGATGAACGTCGTCGTTT+TGG + Chr4:75564309-75564328 None:intergenic 50.0%
! TTGCATTGGTGCTCTCTGCA+TGG - Chr4:75564924-75564943 MsG0480022694.01.T01:CDS 50.0%
!! GAATCTGGGTGATGATGGAG+AGG + Chr4:75564348-75564367 None:intergenic 50.0%
!! GTGAAGTTGGTGCTGCTGAT+AGG - Chr4:75566016-75566035 MsG0480022694.01.T01:intron 50.0%
ATCTCCGCATCCATGGCTGT+TGG + Chr4:75566088-75566107 None:intergenic 55.0%
CTGACAACAACCTTCCTCCC+TGG - Chr4:75566658-75566677 MsG0480022694.01.T01:CDS 55.0%
GAGGGAGCTTCCATTGCAAC+TGG + Chr4:75564329-75564348 None:intergenic 55.0%
GCGGTCCTGACCGTAATGTT+TGG - Chr4:75566416-75566435 MsG0480022694.01.T01:intron 55.0%
!! AACCAAGCACTGGCTACTCC+GGG - Chr4:75566124-75566143 MsG0480022694.01.T01:CDS 55.0%
!! AGGGTAGTACGTGATGGACG+CGG - Chr4:75566397-75566416 MsG0480022694.01.T01:intron 55.0%
!! CAACCAAGCACTGGCTACTC+CGG - Chr4:75566123-75566142 MsG0480022694.01.T01:CDS 55.0%
CGGAGTAGCCAGTGCTTGGT+TGG + Chr4:75566125-75566144 None:intergenic 60.0%
GGGTGAAGGGGGTAGCTATG+AGG - Chr4:75564715-75564734 MsG0480022694.01.T01:CDS 60.0%
TGGGCCAACAGCCATGGATG+CGG - Chr4:75566081-75566100 MsG0480022694.01.T01:CDS 60.0%
!! ACCAAGCACTGGCTACTCCG+GGG - Chr4:75566125-75566144 MsG0480022694.01.T01:CDS 60.0%
AACCTTCCTCCCTGGCGTCC+GGG - Chr4:75566666-75566685 MsG0480022694.01.T01:CDS 65.0%
CAACCTTCCTCCCTGGCGTC+CGG - Chr4:75566665-75566684 MsG0480022694.01.T01:CDS 65.0%
TCTTTCAGCCCCGGACGCCA+GGG + Chr4:75566678-75566697 None:intergenic 65.0%
!! CAAGCACTGGCTACTCCGGG+GGG - Chr4:75566127-75566146 MsG0480022694.01.T01:CDS 65.0%
!! CCAAGCACTGGCTACTCCGG+GGG - Chr4:75566126-75566145 MsG0480022694.01.T01:CDS 65.0%
ACCTTCCTCCCTGGCGTCCG+GGG - Chr4:75566667-75566686 MsG0480022694.01.T01:CDS 70.0%
CCCCCGGAGTAGCCAGTGCT+TGG + Chr4:75566129-75566148 None:intergenic 70.0%
CTCTTTCAGCCCCGGACGCC+AGG + Chr4:75566679-75566698 None:intergenic 70.0%
CTGGCGTCCGGGGCTGAAAG+AGG - Chr4:75566677-75566696 MsG0480022694.01.T01:CDS 70.0%
TTCAGCCCCGGACGCCAGGG+AGG + Chr4:75566675-75566694 None:intergenic 75.0%
! GCCCCGGACGCCAGGGAGGA+AGG + Chr4:75566671-75566690 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr4 gene 75564211 75566710 75564211 ID=MsG0480022694.01;Name=MsG0480022694.01
Chr4 mRNA 75564211 75566710 75564211 ID=MsG0480022694.01.T01;Parent=MsG0480022694.01;Name=MsG0480022694.01.T01;_AED=0.49;_eAED=0.57;_QI=0|0.75|0.6|1|0.75|0.8|5|0|308
Chr4 exon 75566476 75566710 75566476 ID=MsG0480022694.01.T01:exon:23014;Parent=MsG0480022694.01.T01
Chr4 exon 75566026 75566237 75566026 ID=MsG0480022694.01.T01:exon:23013;Parent=MsG0480022694.01.T01
Chr4 exon 75564700 75565017 75564700 ID=MsG0480022694.01.T01:exon:23012;Parent=MsG0480022694.01.T01
Chr4 exon 75564468 75564542 75564468 ID=MsG0480022694.01.T01:exon:23011;Parent=MsG0480022694.01.T01
Chr4 exon 75564211 75564297 75564211 ID=MsG0480022694.01.T01:exon:23010;Parent=MsG0480022694.01.T01
Chr4 CDS 75566476 75566710 75566476 ID=MsG0480022694.01.T01:cds;Parent=MsG0480022694.01.T01
Chr4 CDS 75566026 75566237 75566026 ID=MsG0480022694.01.T01:cds;Parent=MsG0480022694.01.T01
Chr4 CDS 75564700 75565017 75564700 ID=MsG0480022694.01.T01:cds;Parent=MsG0480022694.01.T01
Chr4 CDS 75564468 75564542 75564468 ID=MsG0480022694.01.T01:cds;Parent=MsG0480022694.01.T01
Chr4 CDS 75564211 75564297 75564211 ID=MsG0480022694.01.T01:cds;Parent=MsG0480022694.01.T01
Gene Sequence

>MsG0480022694.01.T01

ATGTGGTATCTATGTGTTTTCTTTCACAGGTTATTGGATTACAGAAAAACCGAAGTCGAATCTTTAGCCAAATTGTTTGGAGCTTTTGATGACAACCAAAACGACGACGTTCATCCCAGTTGCAATGGAAGCTCCCTCTCCATCATCACCCAGATTCTCCTTTCCATTTGGTCAATCTCCCTTCAGAAGAAATTGCTCGTAACATTGCTAATCGAAGTTAGTTTCACTTTCACTTGTATACTTGTGAAGGGAATGTATGAGCTATGGGGTGAAGGGGGTAGCTATGAGGAGTTGAAGGATTCTATATTGAGTTATCCAGATGAGAGAAAGTTGCCATACTTGGAATCTAACAGCACTTTCAGGATTACTGTTGATACCTTTGGGAAGGTTATTAGTCTCCAGGAGCATAAGGAACTCATTCAAGGGTTTACTTATATCCCTTTCAAGGGAAAAGTAAATTTGAGAAAACCAGAGCACAACTTCTGGCTGATAGAAATTGATAATTGTGAAGGTAATAATGGTCTTCCTCCAGTTGAACAAAAGAGAATTTTCTTTGGTCGTGAAGTTGGTGCTGCTGATAGGAAGCTTTTACCGACATACGAGTTGAAAAGCCCGTACTTATCTTGGGCCAACAGCCATGGATGCGGAGATAGCTTTTCTAATGGCCAACCAAGCACTGGCTACTCCGGGGGGAAACTAGTTTATGACCCTTTGTTGGAACCGGAAGTATTTGTTGCAGCAGCTCATTTTGGAGCAATGACCATGGGTGCTGACATTGACATTAGGGTAGTACGTGATGGACGCGGTCCTGACCGTAATGTTTGGAGTAATTTTAAGCAGTATGGATTGGAAATGCCAGTTGGTCTTTTAAGGGCTGACAACAACCTTCCTCCCTGGCGTCCGGGGCTGAAAGAGGTAAATTTTTGA
Protein sequence

>MsG0480022694.01.T01

MWYLCVFFHRLLDYRKTEVESLAKLFGAFDDNQNDDVHPSCNGSSLSIITQILLSIWSISLQKKLLVTLLIEVSFTFTCILVKGMYELWGEGGSYEELKDSILSYPDERKLPYLESNSTFRITVDTFGKVISLQEHKELIQGFTYIPFKGKVNLRKPEHNFWLIEIDNCEGNNGLPPVEQKRIFFGREVGAADRKLLPTYELKSPYLSWANSHGCGDSFSNGQPSTGYSGGKLVYDPLLEPEVFVAAAHFGAMTMGADIDIRVVRDGRGPDRNVWSNFKQYGLEMPVGLLRADNNLPPWRPGLKEVNF*