AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023205.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023205.01.T01 MTR_8g089420 95.299 234 9 2 1 233 139 371 1.92e-163 460
MsG0480023205.01.T01 MTR_2g018990 76.695 236 49 2 1 234 143 374 3.99e-130 375
MsG0480023205.01.T01 MTR_4g113790 54.733 243 93 3 1 226 152 394 2.31e-88 270
MsG0480023205.01.T01 MTR_2g027240 53.469 245 94 5 2 226 261 505 1.17e-84 263
MsG0480023205.01.T01 MTR_6g004720 54.430 237 95 3 2 225 308 544 3.41e-79 250
MsG0480023205.01.T01 MTR_2g062850 52.301 239 100 2 1 225 225 463 4.36e-79 248
MsG0480023205.01.T01 MTR_8g006870 48.327 269 108 4 1 238 426 694 5.62e-79 252
MsG0480023205.01.T01 MTR_1g060790 51.883 239 101 4 1 225 526 764 4.52e-78 251
MsG0480023205.01.T01 MTR_7g033640 51.429 245 99 3 2 226 289 533 8.13e-78 246
MsG0480023205.01.T01 MTR_3g010260 47.970 271 108 6 1 238 471 741 8.18e-77 247
MsG0480023205.01.T01 MTR_7g081665 46.269 268 114 3 1 238 420 687 9.55e-77 246
MsG0480023205.01.T01 MTR_2g069990 52.778 216 87 4 2 217 151 351 8.42e-76 237
MsG0480023205.01.T01 MTR_7g104180 46.840 269 112 5 1 238 548 816 1.95e-75 244
MsG0480023205.01.T01 MTR_2g085065 50.000 236 106 4 2 225 149 384 4.03e-73 230
MsG0480023205.01.T01 MTR_8g446990 50.000 256 110 8 1 239 188 442 5.40e-73 231
MsG0480023205.01.T01 MTR_8g070530 46.522 230 90 4 1 229 653 850 7.45e-64 214
MsG0480023205.01.T01 MTR_2g095980 42.358 229 109 3 1 229 427 632 1.42e-55 189
MsG0480023205.01.T01 MTR_2g095980 42.358 229 109 3 1 229 682 887 7.00e-55 189
MsG0480023205.01.T01 MTR_2g095980 42.358 229 109 3 1 229 682 887 7.43e-55 189
MsG0480023205.01.T01 MTR_8g011780 42.534 221 125 2 2 220 85 305 9.08e-54 179
MsG0480023205.01.T01 MTR_7g053200 40.455 220 128 1 2 218 75 294 3.91e-52 174
MsG0480023205.01.T01 MTR_4g061610 42.358 229 93 5 1 225 694 887 4.49e-52 181
MsG0480023205.01.T01 MTR_4g065080 39.623 265 132 6 2 238 77 341 4.53e-52 174
MsG0480023205.01.T01 MTR_2g098240 39.111 225 117 4 2 224 71 277 1.69e-49 166
MsG0480023205.01.T01 MTR_5g069000 34.667 225 116 6 4 227 820 1014 1.91e-33 127
MsG0480023205.01.T01 MTR_4g035835 34.685 222 115 5 4 225 816 1007 2.31e-33 127
MsG0480023205.01.T01 MTR_6g470940 33.036 224 119 5 4 226 934 1127 3.32e-30 118
MsG0480023205.01.T01 MTR_1g075880 30.435 230 133 6 2 226 172 379 9.90e-29 113
MsG0480023205.01.T01 MTR_2g040150 32.479 234 126 8 10 233 176 387 1.00e-27 110
MsG0480023205.01.T01 MTR_7g073520 29.018 224 98 3 2 225 203 365 1.35e-27 110
MsG0480023205.01.T01 MTR_7g097150 31.092 238 131 8 2 233 175 385 1.67e-27 110
MsG0480023205.01.T01 MTR_1g090917 31.858 226 102 5 1 225 105 279 1.81e-27 109
MsG0480023205.01.T01 MTR_1g090917 31.858 226 102 5 1 225 105 279 1.98e-27 109
MsG0480023205.01.T01 MTR_3g104700 30.396 227 129 6 10 226 157 364 2.54e-27 109
MsG0480023205.01.T01 MTR_1g069735 30.396 227 136 6 2 226 175 381 4.55e-27 108
MsG0480023205.01.T01 MTR_1g069735 30.396 227 136 6 2 226 175 381 4.97e-27 108
MsG0480023205.01.T01 MTR_1g069735 30.396 227 136 6 2 226 175 381 8.77e-27 108
MsG0480023205.01.T01 MTR_1g019340 29.956 227 139 6 2 226 163 371 1.70e-26 107
MsG0480023205.01.T01 MTR_3g104700 30.882 204 114 5 10 205 157 341 2.99e-26 106
MsG0480023205.01.T01 MTR_1g019340 29.956 227 139 6 2 226 163 371 3.58e-26 106
MsG0480023205.01.T01 MTR_1g042700 29.167 240 138 8 2 232 170 386 4.08e-25 103
MsG0480023205.01.T01 MTR_1g442790 30.000 230 135 6 2 226 165 373 5.93e-25 102
MsG0480023205.01.T01 MTR_1g442790 30.000 230 135 6 2 226 165 373 6.29e-25 102
MsG0480023205.01.T01 MTR_1g090917 31.707 205 88 5 22 225 1 154 4.46e-24 99.0
MsG0480023205.01.T01 MTR_1g090917 31.707 205 88 5 22 225 1 154 4.56e-24 99.0
MsG0480023205.01.T01 MTR_2g020380 29.412 221 100 4 2 221 53 218 9.98e-20 88.2
MsG0480023205.01.T01 MTR_4g029020 25.991 227 108 5 1 226 66 233 1.72e-15 75.5
MsG0480023205.01.T01 MTR_7g072575 26.991 226 105 5 1 225 66 232 1.06e-14 73.2
MsG0480023205.01.T01 MTR_4g107280 39.560 91 53 1 4 94 153 241 1.12e-14 72.8
MsG0480023205.01.T01 MTR_4g107280 41.791 67 39 0 159 225 247 313 4.21e-13 68.6
MsG0480023205.01.T01 MTR_4g005730 30.622 209 85 6 1 206 76 227 2.25e-14 72.4
MsG0480023205.01.T01 MTR_4g005730 30.622 209 85 6 1 206 76 227 2.32e-14 72.4
MsG0480023205.01.T01 MTR_4g005730 30.622 209 85 6 1 206 76 227 2.39e-14 72.4
MsG0480023205.01.T01 MTR_2g013900 31.304 115 75 2 21 135 238 348 8.31e-14 70.5
MsG0480023205.01.T01 MTR_1g076370 25.728 206 92 6 22 225 97 243 2.99e-13 68.9
MsG0480023205.01.T01 MTR_6g006770 29.048 210 87 7 1 206 68 219 3.46e-13 68.9
MsG0480023205.01.T01 MTR_4g107280 41.791 67 39 0 159 225 208 274 4.73e-13 68.2
MsG0480023205.01.T01 MTR_4g107280 42.466 73 40 1 22 94 132 202 1.43e-12 66.6
MsG0480023205.01.T01 MTR_5g088350 25.221 226 110 5 1 225 67 234 1.14e-12 66.6
MsG0480023205.01.T01 MTR_5g088350 25.221 226 110 5 1 225 67 234 1.27e-12 67.0
MsG0480023205.01.T01 MTR_4g088755 28.358 134 92 2 2 135 97 226 1.45e-12 66.6
MsG0480023205.01.T01 MTR_4g088745 28.358 134 92 2 2 135 102 231 1.46e-12 66.6
MsG0480023205.01.T01 MTR_5g088350 25.221 226 110 5 1 225 67 234 1.54e-12 66.6
MsG0480023205.01.T01 MTR_3g088715 34.737 95 56 1 131 225 1 89 1.60e-12 63.2
MsG0480023205.01.T01 MTR_5g067150 36.842 95 56 2 1 94 63 154 6.95e-12 64.7
MsG0480023205.01.T01 MTR_4g088750 28.696 115 78 2 21 135 277 387 7.28e-12 64.7
MsG0480023205.01.T01 MTR_5g067150 36.842 95 56 2 1 94 63 154 7.75e-12 64.7
MsG0480023205.01.T01 MTR_5g067150 36.842 95 56 2 1 94 63 154 9.52e-12 64.3
MsG0480023205.01.T01 MTR_4g131060 26.214 206 92 5 1 205 72 218 1.02e-11 64.3
MsG0480023205.01.T01 MTR_8g024590 34.021 97 62 1 1 97 71 165 3.63e-11 62.8
MsG0480023205.01.T01 MTR_8g024600 34.021 97 62 1 1 97 71 165 4.03e-11 62.4
MsG0480023205.01.T01 MTR_3g466480 25.778 225 107 5 2 225 76 241 8.19e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023205.01.T01 AT2G34650 71.121 232 54 3 1 229 134 355 6.63e-118 343
MsG0480023205.01.T01 AT4G26610 53.333 240 97 2 2 226 178 417 1.89e-85 263
MsG0480023205.01.T01 AT5G47750 50.916 273 97 6 2 238 246 517 2.24e-83 259
MsG0480023205.01.T01 AT5G55910 53.279 244 95 5 2 226 164 407 4.64e-83 256
MsG0480023205.01.T01 AT5G55910 53.279 244 95 5 2 226 164 407 4.64e-83 256
MsG0480023205.01.T01 AT5G40030 52.101 238 102 4 1 226 168 405 2.58e-82 254
MsG0480023205.01.T01 AT2G26700 47.653 277 92 4 1 225 143 418 6.37e-81 251
MsG0480023205.01.T01 AT3G27580 54.321 243 94 6 2 227 237 479 1.90e-77 244
MsG0480023205.01.T01 AT3G27580 54.321 243 94 6 2 227 237 479 1.90e-77 244
MsG0480023205.01.T01 AT1G16440 51.948 231 104 2 1 226 169 397 4.79e-77 241
MsG0480023205.01.T01 AT3G12690 51.931 233 102 3 1 225 239 469 7.66e-77 243
MsG0480023205.01.T01 AT3G12690 51.931 233 102 3 1 225 239 469 7.66e-77 243
MsG0480023205.01.T01 AT3G12690 51.931 233 102 3 1 225 239 469 7.66e-77 243
MsG0480023205.01.T01 AT1G79250 51.653 242 101 3 1 226 200 441 3.54e-76 240
MsG0480023205.01.T01 AT1G79250 51.653 242 101 3 1 226 200 441 3.54e-76 240
MsG0480023205.01.T01 AT1G79250 51.653 242 101 3 1 226 200 441 3.54e-76 240
MsG0480023205.01.T01 AT1G79250 51.653 242 101 3 1 226 200 441 3.54e-76 240
MsG0480023205.01.T01 AT5G03640 51.220 246 97 4 1 225 595 838 8.23e-76 246
MsG0480023205.01.T01 AT5G03640 51.220 246 97 4 1 225 595 838 8.23e-76 246
MsG0480023205.01.T01 AT1G53700 52.752 218 90 3 1 218 148 352 4.83e-75 235
MsG0480023205.01.T01 AT3G52890 50.400 250 97 4 1 225 592 839 1.04e-74 243
MsG0480023205.01.T01 AT3G52890 50.400 250 97 4 1 225 592 839 1.04e-74 243
MsG0480023205.01.T01 AT3G52890 50.400 250 97 4 1 225 592 839 1.04e-74 243
MsG0480023205.01.T01 AT3G52890 50.400 250 97 4 1 225 592 839 1.04e-74 243
MsG0480023205.01.T01 AT3G44610 48.780 246 98 6 2 225 134 373 5.36e-74 232
MsG0480023205.01.T01 AT3G14370 51.835 218 94 2 1 218 142 348 8.92e-74 232
MsG0480023205.01.T01 AT2G36350 45.487 277 111 5 1 238 613 888 7.36e-73 239
MsG0480023205.01.T01 AT2G44830 49.798 247 98 6 1 226 417 658 2.52e-72 234
MsG0480023205.01.T01 AT2G44830 49.798 247 98 6 1 226 417 658 2.52e-72 234
MsG0480023205.01.T01 AT2G44830 49.798 247 98 6 1 226 417 658 2.52e-72 234
MsG0480023205.01.T01 AT5G58140 45.852 229 93 3 1 229 631 828 1.93e-63 213
MsG0480023205.01.T01 AT5G58140 45.852 229 93 3 1 229 631 828 2.61e-63 212
MsG0480023205.01.T01 AT5G58140 45.852 229 93 3 1 229 631 828 2.61e-63 212
MsG0480023205.01.T01 AT5G58140 45.852 229 93 3 1 229 631 828 2.61e-63 212
MsG0480023205.01.T01 AT5G58140 45.852 229 93 3 1 229 631 828 2.61e-63 212
MsG0480023205.01.T01 AT5G58140 45.852 229 93 3 1 229 631 828 2.61e-63 212
MsG0480023205.01.T01 AT3G45780 41.333 225 103 3 1 225 717 912 8.06e-53 183
MsG0480023205.01.T01 AT3G45780 41.333 225 103 3 1 225 717 912 8.06e-53 183
MsG0480023205.01.T01 AT3G20830 41.410 227 120 3 4 224 84 303 8.86e-51 171
MsG0480023205.01.T01 AT1G51170 41.304 230 114 5 4 224 85 302 1.21e-49 167
MsG0480023205.01.T01 AT3G25250 39.091 220 126 4 3 221 80 292 2.52e-47 162
MsG0480023205.01.T01 AT4G13000 39.091 220 124 3 2 221 76 285 6.78e-46 157
MsG0480023205.01.T01 AT3G17850 35.268 224 114 5 4 226 939 1132 4.92e-33 127
MsG0480023205.01.T01 AT1G48490 32.489 237 122 6 4 239 885 1084 1.64e-32 125
MsG0480023205.01.T01 AT1G48490 32.489 237 122 6 4 239 885 1084 1.64e-32 125
MsG0480023205.01.T01 AT1G48490 32.489 237 122 6 4 239 885 1084 1.64e-32 125
MsG0480023205.01.T01 AT1G48490 32.489 237 122 6 4 239 886 1085 1.65e-32 125
MsG0480023205.01.T01 AT5G62310 33.632 223 119 5 4 226 811 1004 4.07e-31 121
MsG0480023205.01.T01 AT1G45160 34.685 222 112 5 4 225 727 915 4.43e-30 118
MsG0480023205.01.T01 AT1G45160 34.685 222 112 5 4 225 727 915 6.10e-30 117
MsG0480023205.01.T01 AT4G33080 31.466 232 128 7 6 226 153 364 1.08e-29 116
MsG0480023205.01.T01 AT4G33080 31.466 232 128 7 6 226 153 364 1.29e-29 115
MsG0480023205.01.T01 AT5G04510 31.858 226 102 5 2 226 99 273 1.80e-28 109
MsG0480023205.01.T01 AT5G04510 31.718 227 103 5 1 226 98 273 1.38e-27 109
MsG0480023205.01.T01 AT5G04510 31.718 227 103 5 1 226 98 273 1.61e-27 110
MsG0480023205.01.T01 AT3G08730 31.111 225 93 5 2 226 189 351 1.96e-27 109
MsG0480023205.01.T01 AT3G08730 31.111 225 93 5 2 226 189 351 1.96e-27 109
MsG0480023205.01.T01 AT4G14350 30.973 226 135 6 2 225 174 380 2.31e-27 109
MsG0480023205.01.T01 AT4G14350 30.973 226 135 6 2 225 174 380 2.31e-27 109
MsG0480023205.01.T01 AT4G14350 30.973 226 135 6 2 225 174 380 2.31e-27 109
MsG0480023205.01.T01 AT4G14350 30.973 226 135 6 2 225 174 380 2.49e-27 109
MsG0480023205.01.T01 AT3G08720 31.111 225 93 5 2 226 195 357 3.62e-27 108
MsG0480023205.01.T01 AT3G08720 31.111 225 93 5 2 226 195 357 3.62e-27 108
MsG0480023205.01.T01 AT3G08720 31.111 225 93 5 2 226 195 357 3.62e-27 108
MsG0480023205.01.T01 AT3G08720 31.111 225 93 5 2 226 195 357 3.62e-27 108
MsG0480023205.01.T01 AT2G19400 31.224 237 129 8 10 232 168 384 1.62e-26 107
MsG0480023205.01.T01 AT3G10540 30.837 227 105 5 1 226 99 274 1.97e-26 107
MsG0480023205.01.T01 AT1G03920 30.837 227 135 6 2 226 79 285 2.87e-26 106
MsG0480023205.01.T01 AT2G20470 31.897 232 125 6 2 226 179 384 2.91e-26 106
MsG0480023205.01.T01 AT2G20470 31.897 232 125 6 2 226 179 384 3.61e-26 106
MsG0480023205.01.T01 AT2G20470 31.897 232 125 6 2 226 179 384 3.61e-26 106
MsG0480023205.01.T01 AT1G03920 30.837 227 135 6 2 226 192 398 5.22e-26 106
MsG0480023205.01.T01 AT1G03920 30.837 227 135 6 2 226 192 398 5.22e-26 106
MsG0480023205.01.T01 AT3G23310 30.702 228 135 7 2 225 175 383 7.68e-26 105
MsG0480023205.01.T01 AT1G30640 32.018 228 134 7 2 225 82 292 8.42e-26 105
MsG0480023205.01.T01 AT1G30640 32.018 228 134 7 2 225 175 385 1.28e-25 105
MsG0480023205.01.T01 AT1G30640 32.018 228 134 7 2 225 175 385 1.28e-25 105
MsG0480023205.01.T01 AT5G09890 29.258 229 140 7 2 226 157 367 3.76e-23 97.8
MsG0480023205.01.T01 AT5G09890 29.258 229 140 7 2 226 157 367 3.96e-23 97.8
MsG0480023205.01.T01 AT5G18700 44.086 93 50 1 2 94 53 143 2.33e-17 81.3
MsG0480023205.01.T01 AT5G57630 26.106 226 104 6 1 225 66 229 7.14e-15 73.6
MsG0480023205.01.T01 AT5G57630 26.106 226 104 6 1 225 66 229 8.29e-15 73.2
MsG0480023205.01.T01 AT5G45810 26.872 227 102 5 4 225 85 252 1.15e-14 73.2
MsG0480023205.01.T01 AT1G30270 25.221 226 110 5 1 225 85 252 1.43e-13 70.1
MsG0480023205.01.T01 AT1G30270 25.221 226 110 5 1 225 85 252 1.45e-13 69.7
MsG0480023205.01.T01 AT4G24400 27.885 208 85 7 1 205 63 208 3.36e-13 68.6
MsG0480023205.01.T01 AT4G24400 27.885 208 85 7 1 205 63 208 3.56e-13 68.6
MsG0480023205.01.T01 AT1G12680 36.957 92 56 1 4 95 158 247 5.03e-13 68.2
MsG0480023205.01.T01 AT5G21326 36.634 101 60 2 1 100 67 164 7.64e-13 67.8
MsG0480023205.01.T01 AT2G45490 35.714 98 58 1 133 225 140 237 7.78e-13 66.6
MsG0480023205.01.T01 AT2G45490 35.714 98 58 1 133 225 140 237 1.00e-12 66.6
MsG0480023205.01.T01 AT5G57565 38.202 89 48 2 21 109 33 114 4.28e-12 62.0
MsG0480023205.01.T01 AT5G57565 38.202 89 48 2 21 109 33 114 4.28e-12 62.0
MsG0480023205.01.T01 AT2G20050 36.000 75 46 1 153 225 926 1000 1.03e-11 64.7
MsG0480023205.01.T01 AT2G20050 36.000 75 46 1 153 225 923 997 1.07e-11 64.7
MsG0480023205.01.T01 AT5G58950 32.258 93 63 0 2 94 265 357 1.53e-11 63.9
MsG0480023205.01.T01 AT2G30360 35.354 99 62 1 2 100 77 173 2.25e-11 63.5
MsG0480023205.01.T01 AT4G14480 38.298 94 58 0 1 94 68 161 2.55e-11 63.2
MsG0480023205.01.T01 AT2G26980 33.000 100 65 1 1 100 68 165 3.65e-11 62.8
MsG0480023205.01.T01 AT2G26980 33.000 100 65 1 1 100 78 175 3.70e-11 62.8
MsG0480023205.01.T01 AT2G26980 33.000 100 65 1 1 100 68 165 3.88e-11 62.4
MsG0480023205.01.T01 AT5G35410 36.842 95 56 2 1 94 65 156 4.23e-11 62.4
MsG0480023205.01.T01 AT2G26980 33.000 100 65 1 1 100 68 165 4.97e-11 61.6
MsG0480023205.01.T01 AT2G26980 33.000 100 65 1 1 100 68 165 5.10e-11 62.4
MsG0480023205.01.T01 AT5G35410 36.842 95 56 2 1 94 65 156 5.87e-11 62.0
MsG0480023205.01.T01 AT3G46930 36.000 75 48 0 20 94 274 348 5.89e-11 62.4
MsG0480023205.01.T01 AT5G57565 34.146 123 69 4 21 143 33 143 6.00e-11 60.1
MsG0480023205.01.T01 AT5G57565 38.202 89 46 3 21 109 33 112 6.54e-11 59.3
MsG0480023205.01.T01 AT5G57565 38.202 89 46 3 21 109 33 112 6.54e-11 59.3
MsG0480023205.01.T01 AT1G29230 32.990 97 62 1 4 100 131 224 6.55e-11 62.0
MsG0480023205.01.T01 AT3G46930 36.000 75 48 0 20 94 274 348 6.55e-11 62.0
MsG0480023205.01.T01 AT3G46930 36.000 75 48 0 20 94 239 313 6.56e-11 62.0
MsG0480023205.01.T01 AT4G32660 26.866 201 109 5 14 214 27 189 6.89e-11 61.2
MsG0480023205.01.T01 AT3G46930 36.000 75 48 0 20 94 274 348 7.80e-11 61.6
MsG0480023205.01.T01 AT3G46930 36.000 75 48 0 20 94 239 313 9.00e-11 61.6
MsG0480023205.01.T01 AT3G46930 36.000 75 48 0 20 94 178 252 9.15e-11 61.2

Find 59 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGTTGCCGTGGGAGTTTC+CGG 0.194727 4:-82165883 None:intergenic
TCATTTCTCTTGCATTGTTA+TGG 0.264166 4:+82165303 MsG0480023205.01.T01:CDS
GCCCGCTCTTGTTCATTTGT+TGG 0.268785 4:+82165828 MsG0480023205.01.T01:CDS
TTTATCTATGAACTTATTTA+CGG 0.276743 4:+82165930 MsG0480023205.01.T01:CDS
GCCGTGGATTGGTGGTCGTT+TGG 0.326507 4:+82165903 MsG0480023205.01.T01:CDS
GTTTGCGGCTCCATCGAATA+AGG 0.328263 4:+82165962 MsG0480023205.01.T01:CDS
TTCAAGCGTGCTTGAGGCCG+TGG 0.353163 4:-82166029 None:intergenic
CCGTGGATTGGTGGTCGTTT+GGG 0.365851 4:+82165904 MsG0480023205.01.T01:CDS
GAGTTTCCGGCAGCAACTTC+CGG 0.366200 4:-82165870 None:intergenic
CCTTACGTGACTTGAATAAC+CGG 0.404078 4:-82165758 None:intergenic
TTACGGTCGTACTCCGTTTG+CGG 0.414214 4:+82165947 MsG0480023205.01.T01:CDS
TTCAAACCGTTCAACCAAAC+CGG 0.428406 4:+82165781 MsG0480023205.01.T01:CDS
GCATAGAGTGTAGGAAGAAA+AGG 0.432484 4:-82165265 None:intergenic
GTGAGGGTGTTTGTGGCGTA+TGG 0.435424 4:-82165353 None:intergenic
CGCAGTGTTTCCTTATTCGA+TGG 0.435512 4:-82165972 None:intergenic
TTATCTGATGATTCAACGGC+TGG 0.439572 4:-82165651 None:intergenic
GAACAAGAGCGGGCAGAAAC+CGG 0.457262 4:-82165819 None:intergenic
CGTGCTTGAGGCCGTGGGTG+TGG 0.466165 4:-82166023 None:intergenic
GATCGAACTAACACATTCTC+AGG 0.472536 4:-82165573 None:intergenic
TTATTCGATGGAGCCGCAAA+CGG 0.483287 4:-82165960 None:intergenic
GACGGTTGTGAGGGTGTTTG+TGG 0.483934 4:-82165360 None:intergenic
ACCAATCCACGGCGTTGCCG+TGG 0.489785 4:-82165893 None:intergenic
CCCACGGCAACGCCGTGGAT+TGG 0.491974 4:+82165892 MsG0480023205.01.T01:CDS
CGTGGGTGTGGAAAAGTAAG+AGG 0.499315 4:-82166011 None:intergenic
ACGGCAACGCCGTGGATTGG+TGG 0.504904 4:+82165895 MsG0480023205.01.T01:CDS
ATTTACCTAGCGGAAGTAAG+AGG 0.510467 4:-82165385 None:intergenic
GAATGGTGTAGGAAAAGAAC+GGG 0.518949 4:-82165721 None:intergenic
AGCGGAAGTATTGGTGGCAT+TGG 0.529444 4:+82165509 MsG0480023205.01.T01:CDS
TCCAACAAATGAACAAGAGC+GGG 0.536839 4:-82165829 None:intergenic
TAAATTATCTGATGATTCAA+CGG 0.538115 4:-82165655 None:intergenic
TAAGAGGAAGACGGTTGTGA+GGG 0.539144 4:-82165369 None:intergenic
TTATGCAGCGGAAGTATTGG+TGG 0.540414 4:+82165503 MsG0480023205.01.T01:CDS
TTGGAGTACCTTCACATGCT+AGG 0.543818 4:+82165528 MsG0480023205.01.T01:CDS
GTAAGAGGAAGACGGTTGTG+AGG 0.548424 4:-82165370 None:intergenic
CGGTTTGAAAAGCAAGTGAA+TGG 0.548672 4:-82165738 None:intergenic
GAAAAGCAAGTGAATGGTGT+AGG 0.548777 4:-82165732 None:intergenic
CCGGTTATTCAAGTCACGTA+AGG 0.551242 4:+82165758 MsG0480023205.01.T01:CDS
GTCTCGCCGGAAGTTGCTGC+CGG 0.568773 4:+82165864 MsG0480023205.01.T01:CDS
AGAACGGGAACGAGTGTAGG+AGG 0.569045 4:-82165706 None:intergenic
TCAAACTCAGCATAGAGTGT+AGG 0.584379 4:-82165274 None:intergenic
GTAGAGTTCAAGCGTGCTTG+AGG 0.584583 4:-82166035 None:intergenic
TCAAGCGTGCTTGAGGCCGT+GGG 0.588656 4:-82166028 None:intergenic
AAAAGAACGGGAACGAGTGT+AGG 0.591056 4:-82165709 None:intergenic
AACACATGAATATGTCTCGC+CGG 0.593503 4:+82165851 MsG0480023205.01.T01:CDS
TATCTGATGATTCAACGGCT+GGG 0.596183 4:-82165650 None:intergenic
CCAATCCACGGCGTTGCCGT+GGG 0.598052 4:-82165892 None:intergenic
GTTGCTGCCGGAAACTCCCA+CGG 0.601452 4:+82165876 MsG0480023205.01.T01:CDS
CACGCTTGAACTCTACGCGC+GGG 0.603495 4:+82166042 MsG0480023205.01.T01:CDS
CCCAAACGACCACCAATCCA+CGG 0.607776 4:-82165904 None:intergenic
TATGGAATGCAAGTCACCAC+CGG 0.613302 4:-82165335 None:intergenic
AAACTCCCACGGCAACGCCG+TGG 0.617090 4:+82165887 MsG0480023205.01.T01:CDS
TTCCAACAAATGAACAAGAG+CGG 0.641367 4:-82165830 None:intergenic
AATGTGTTAGTTCGATCAGA+CGG 0.646609 4:+82165579 MsG0480023205.01.T01:CDS
AAATGATTCCTAGCATGTGA+AGG 0.647952 4:-82165536 None:intergenic
GCACGCTTGAACTCTACGCG+CGG 0.659257 4:+82166041 MsG0480023205.01.T01:CDS
TGAATGGTGTAGGAAAAGAA+CGG 0.666750 4:-82165722 None:intergenic
GTCTTCCTCTTACTTCCGCT+AGG 0.667703 4:+82165380 MsG0480023205.01.T01:CDS
TAGCGGAAGTAAGAGGAAGA+CGG 0.675534 4:-82165378 None:intergenic
GAACGGGAACGAGTGTAGGA+GGG 0.698412 4:-82165705 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATCTATGAACTTATTTA+CGG + Chr4:82165930-82165949 MsG0480023205.01.T01:CDS 15.0%
!! TAAATTATCTGATGATTCAA+CGG - Chr4:82165658-82165677 None:intergenic 20.0%
!! ATTGTTATGGAATTTTGTTC+CGG + Chr4:82165316-82165335 MsG0480023205.01.T01:CDS 25.0%
TCATTTCTCTTGCATTGTTA+TGG + Chr4:82165303-82165322 MsG0480023205.01.T01:CDS 30.0%
AAATGATTCCTAGCATGTGA+AGG - Chr4:82165539-82165558 None:intergenic 35.0%
AATGTGTTAGTTCGATCAGA+CGG + Chr4:82165579-82165598 MsG0480023205.01.T01:CDS 35.0%
TGAATGGTGTAGGAAAAGAA+CGG - Chr4:82165725-82165744 None:intergenic 35.0%
TTCCAACAAATGAACAAGAG+CGG - Chr4:82165833-82165852 None:intergenic 35.0%
! GGGTAATGTTGATGAAAAAG+TGG - Chr4:82165688-82165707 None:intergenic 35.0%
! GTTACTCAGATTTTATGCAG+CGG + Chr4:82165491-82165510 MsG0480023205.01.T01:intron 35.0%
!! ATTTTATGCAGCGGAAGTAT+TGG + Chr4:82165500-82165519 MsG0480023205.01.T01:CDS 35.0%
!! CATTCACTTGCTTTTCAAAC+CGG + Chr4:82165739-82165758 MsG0480023205.01.T01:CDS 35.0%
!!! GTTTTGAGAGATTTACCTAG+CGG - Chr4:82165398-82165417 None:intergenic 35.0%
AACACATGAATATGTCTCGC+CGG + Chr4:82165851-82165870 MsG0480023205.01.T01:CDS 40.0%
ATTTACCTAGCGGAAGTAAG+AGG - Chr4:82165388-82165407 None:intergenic 40.0%
CCTTACGTGACTTGAATAAC+CGG - Chr4:82165761-82165780 None:intergenic 40.0%
CGGTTTGAAAAGCAAGTGAA+TGG - Chr4:82165741-82165760 None:intergenic 40.0%
GAAAAGCAAGTGAATGGTGT+AGG - Chr4:82165735-82165754 None:intergenic 40.0%
GAATGGTGTAGGAAAAGAAC+GGG - Chr4:82165724-82165743 None:intergenic 40.0%
GATCGAACTAACACATTCTC+AGG - Chr4:82165576-82165595 None:intergenic 40.0%
GCATAGAGTGTAGGAAGAAA+AGG - Chr4:82165268-82165287 None:intergenic 40.0%
TATCTGATGATTCAACGGCT+GGG - Chr4:82165653-82165672 None:intergenic 40.0%
TCAAACTCAGCATAGAGTGT+AGG - Chr4:82165277-82165296 None:intergenic 40.0%
TCCAACAAATGAACAAGAGC+GGG - Chr4:82165832-82165851 None:intergenic 40.0%
TTATCTGATGATTCAACGGC+TGG - Chr4:82165654-82165673 None:intergenic 40.0%
TTCAAACCGTTCAACCAAAC+CGG + Chr4:82165781-82165800 MsG0480023205.01.T01:CDS 40.0%
! GTTATGGAATTTTGTTCCGG+TGG + Chr4:82165319-82165338 MsG0480023205.01.T01:CDS 40.0%
!! AAAAAGCCGGTTTGGTTGAA+CGG - Chr4:82165790-82165809 None:intergenic 40.0%
AAAAGAACGGGAACGAGTGT+AGG - Chr4:82165712-82165731 None:intergenic 45.0%
CCGGTTATTCAAGTCACGTA+AGG + Chr4:82165758-82165777 MsG0480023205.01.T01:CDS 45.0%
CGCAGTGTTTCCTTATTCGA+TGG - Chr4:82165975-82165994 None:intergenic 45.0%
TAAGAGGAAGACGGTTGTGA+GGG - Chr4:82165372-82165391 None:intergenic 45.0%
TAGCGGAAGTAAGAGGAAGA+CGG - Chr4:82165381-82165400 None:intergenic 45.0%
TATGGAATGCAAGTCACCAC+CGG - Chr4:82165338-82165357 None:intergenic 45.0%
TCAGCTACAAAAAGCCGGTT+TGG - Chr4:82165798-82165817 None:intergenic 45.0%
TTATTCGATGGAGCCGCAAA+CGG - Chr4:82165963-82165982 None:intergenic 45.0%
! TTGGAGTACCTTCACATGCT+AGG + Chr4:82165528-82165547 MsG0480023205.01.T01:CDS 45.0%
!! CTCTTACTTTTCCACACCCA+CGG + Chr4:82166012-82166031 MsG0480023205.01.T01:CDS 45.0%
!! TTATGCAGCGGAAGTATTGG+TGG + Chr4:82165503-82165522 MsG0480023205.01.T01:CDS 45.0%
CCGGTTCAGCTACAAAAAGC+CGG - Chr4:82165803-82165822 None:intergenic 50.0%
CGTGGGTGTGGAAAAGTAAG+AGG - Chr4:82166014-82166033 None:intergenic 50.0%
GCCCGCTCTTGTTCATTTGT+TGG + Chr4:82165828-82165847 MsG0480023205.01.T01:CDS 50.0%
GTAAGAGGAAGACGGTTGTG+AGG - Chr4:82165373-82165392 None:intergenic 50.0%
GTAGAGTTCAAGCGTGCTTG+AGG - Chr4:82166038-82166057 None:intergenic 50.0%
GTCTTCCTCTTACTTCCGCT+AGG + Chr4:82165380-82165399 MsG0480023205.01.T01:CDS 50.0%
GTTTGCGGCTCCATCGAATA+AGG + Chr4:82165962-82165981 MsG0480023205.01.T01:CDS 50.0%
TTACGGTCGTACTCCGTTTG+CGG + Chr4:82165947-82165966 MsG0480023205.01.T01:CDS 50.0%
! CCGGCTTTTTGTAGCTGAAC+CGG + Chr4:82165800-82165819 MsG0480023205.01.T01:CDS 50.0%
!! AGCGGAAGTATTGGTGGCAT+TGG + Chr4:82165509-82165528 MsG0480023205.01.T01:CDS 50.0%
AGAACGGGAACGAGTGTAGG+AGG - Chr4:82165709-82165728 None:intergenic 55.0%
CCCAAACGACCACCAATCCA+CGG - Chr4:82165907-82165926 None:intergenic 55.0%
GAACAAGAGCGGGCAGAAAC+CGG - Chr4:82165822-82165841 None:intergenic 55.0%
GAACGGGAACGAGTGTAGGA+GGG - Chr4:82165708-82165727 None:intergenic 55.0%
GAGTTTCCGGCAGCAACTTC+CGG - Chr4:82165873-82165892 None:intergenic 55.0%
!! CCGTGGATTGGTGGTCGTTT+GGG + Chr4:82165904-82165923 MsG0480023205.01.T01:CDS 55.0%
!! GACGGTTGTGAGGGTGTTTG+TGG - Chr4:82165363-82165382 None:intergenic 55.0%
!! GTGAGGGTGTTTGTGGCGTA+TGG - Chr4:82165356-82165375 None:intergenic 55.0%
CACGCTTGAACTCTACGCGC+GGG + Chr4:82166042-82166061 MsG0480023205.01.T01:CDS 60.0%
GCACGCTTGAACTCTACGCG+CGG + Chr4:82166041-82166060 MsG0480023205.01.T01:CDS 60.0%
GTTGCTGCCGGAAACTCCCA+CGG + Chr4:82165876-82165895 MsG0480023205.01.T01:CDS 60.0%
TCAAGCGTGCTTGAGGCCGT+GGG - Chr4:82166031-82166050 None:intergenic 60.0%
TTCAAGCGTGCTTGAGGCCG+TGG - Chr4:82166032-82166051 None:intergenic 60.0%
!! GCCGTGGATTGGTGGTCGTT+TGG + Chr4:82165903-82165922 MsG0480023205.01.T01:CDS 60.0%
AAACTCCCACGGCAACGCCG+TGG + Chr4:82165887-82165906 MsG0480023205.01.T01:CDS 65.0%
ACCAATCCACGGCGTTGCCG+TGG - Chr4:82165896-82165915 None:intergenic 65.0%
CCAATCCACGGCGTTGCCGT+GGG - Chr4:82165895-82165914 None:intergenic 65.0%
GGCGTTGCCGTGGGAGTTTC+CGG - Chr4:82165886-82165905 None:intergenic 65.0%
GTCTCGCCGGAAGTTGCTGC+CGG + Chr4:82165864-82165883 MsG0480023205.01.T01:CDS 65.0%
!! ACGGCAACGCCGTGGATTGG+TGG + Chr4:82165895-82165914 MsG0480023205.01.T01:CDS 65.0%
CCCACGGCAACGCCGTGGAT+TGG + Chr4:82165892-82165911 MsG0480023205.01.T01:CDS 70.0%
CGTGCTTGAGGCCGTGGGTG+TGG - Chr4:82166026-82166045 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 82165253 82166070 82165253 ID=MsG0480023205.01;Name=MsG0480023205.01
Chr4 mRNA 82165253 82166070 82165253 ID=MsG0480023205.01.T01;Parent=MsG0480023205.01;Name=MsG0480023205.01.T01;_AED=0.41;_eAED=0.41;_QI=0|0|0|1|1|1|2|0|239
Chr4 exon 82165253 82165401 82165253 ID=MsG0480023205.01.T01:exon:880;Parent=MsG0480023205.01.T01
Chr4 exon 82165500 82166070 82165500 ID=MsG0480023205.01.T01:exon:881;Parent=MsG0480023205.01.T01
Chr4 CDS 82165253 82165401 82165253 ID=MsG0480023205.01.T01:cds;Parent=MsG0480023205.01.T01
Chr4 CDS 82165500 82166070 82165500 ID=MsG0480023205.01.T01:cds;Parent=MsG0480023205.01.T01
Gene Sequence

>MsG0480023205.01.T01

ATGCTTGATCATCCTTTTCTTCCTACACTCTATGCTGAGTTTGAAGCTTCTCATTTCTCTTGCATTGTTATGGAATTTTGTTCCGGTGGTGACTTGCATTCCATACGCCACAAACACCCTCACAACCGTCTTCCTCTTACTTCCGCTAGATTTTATGCAGCGGAAGTATTGGTGGCATTGGAGTACCTTCACATGCTAGGAATCATTTACAGAGATCTAAAGCCTGAGAATGTGTTAGTTCGATCAGACGGTCACATTATGCTTTCAGATTTCGATCTTTCACTTTGCTCAAATGCAATCCCAGCCGTTGAATCATCAGATAATTTACAAGATTCCACTTTTTCATCAACATTACCCTCCTACACTCGTTCCCGTTCTTTTCCTACACCATTCACTTGCTTTTCAAACCGGTTATTCAAGTCACGTAAGGTTCAAACCGTTCAACCAAACCGGCTTTTTGTAGCTGAACCGGTTTCTGCCCGCTCTTGTTCATTTGTTGGAACACATGAATATGTCTCGCCGGAAGTTGCTGCCGGAAACTCCCACGGCAACGCCGTGGATTGGTGGTCGTTTGGGATATTTATCTATGAACTTATTTACGGTCGTACTCCGTTTGCGGCTCCATCGAATAAGGAAACACTGCGTAACATCTTAAAAAAGCCTCTTACTTTTCCACACCCACGGCCTCAAGCACGCTTGAACTCTACGCGCGGGATTTGA
Protein sequence

>MsG0480023205.01.T01

MLDHPFLPTLYAEFEASHFSCIVMEFCSGGDLHSIRHKHPHNRLPLTSARFYAAEVLVALEYLHMLGIIYRDLKPENVLVRSDGHIMLSDFDLSLCSNAIPAVESSDNLQDSTFSSTLPSYTRSRSFPTPFTCFSNRLFKSRKVQTVQPNRLFVAEPVSARSCSFVGTHEYVSPEVAAGNSHGNAVDWWSFGIFIYELIYGRTPFAAPSNKETLRNILKKPLTFPHPRPQARLNSTRGI*