AlfalfaGEDB | MsG0480023292.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023292.01

KEGG: K15334
GO: GO:0003723 GO:0008168 GO:0016428
PFAM: PF01189

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023292.01.T01	XP_024628298.1	83.86	570	21	4	1	499	25	594	0	957
MsG0480023292.01.T01	XP_024628298.1	96.396	111	4	0	552	662	736	846	2.23E-56	217

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023292.01.T01	Q5ZLV4	45.409	403	184	5	46	421	41	434	5.81E-107	345

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023292.01.T01	A0A396GP25	84.629	566	20	3	1	499	25	590	0.0	945

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480023258.01	MsG0480023292.01	0.806316	8.729038e-50	4.723062e-47
MsG0480023292.01	MsG0780039623.01	0.814383	1.584464e-51	1.061629e-48
MsG0480023292.01	MsG0780040856.01	0.825575	4.349304e-54	3.979731e-51

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023292.01.T01	MTR_8g089980	84.629	566	20	3	1	499	1	566	0.0	944
MsG0480023292.01.T01	MTR_8g089980	96.396	111	4	0	552	662	708	818	2.58e-60	216
MsG0480023292.01.T01	MTR_3g086950	58.247	582	137	12	1	499	1	559	0.0	619
MsG0480023292.01.T01	MTR_3g086950	60.000	65	26	0	548	612	697	761	4.41e-20	95.5
MsG0480023292.01.T01	MTR_3g460700	25.613	367	177	9	166	499	296	599	1.85e-17	87.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023292.01.T01	AT2G22400	54.641	571	177	13	8	497	11	580	0.0	580
MsG0480023292.01.T01	AT2G22400	38.333	120	62	4	519	633	689	801	4.70e-14	76.3
MsG0480023292.01.T01	AT4G40000	56.641	512	162	6	1	462	1	502	0.0	537
MsG0480023292.01.T01	AT4G40000	47.887	71	37	0	548	618	693	763	1.08e-14	78.2
MsG0480023292.01.T01	AT4G40000	60.082	243	74	4	236	462	55	290	2.33e-82	271
MsG0480023292.01.T01	AT4G40000	47.887	71	37	0	548	618	481	551	1.07e-14	77.8
MsG0480023292.01.T01	AT4G26600	26.909	275	126	7	166	418	327	548	1.26e-14	77.8
MsG0480023292.01.T01	AT4G26600	26.909	275	126	7	166	418	327	548	1.46e-14	77.8
MsG0480023292.01.T01	AT4G26600	29.412	204	108	5	166	347	327	516	1.33e-13	74.3
MsG0480023292.01.T01	AT4G26600	30.978	184	95	4	166	327	327	500	1.71e-13	73.9

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Find 166 sgRNAs with CRISPR-Local

Find 459 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAGGATCACTGCCTGA+TGG	0.612819	4:+83412602	MsG0480023292.01.T01:CDS
AAAGCTGCTGTGAGTTAGAG+AGG	0.571057	4:+83416876	MsG0480023292.01.T01:exon
AAAGTCTTGCAAGCAATTCT+TGG	0.304314	4:-83416737	None:intergenic
AAATGAAGCTGTGGTTGCAG+AGG	0.679409	4:+83414099	MsG0480023292.01.T01:CDS
AAATGGCTCTTCTGGGCAGG+TGG	0.556454	4:+83416779	MsG0480023292.01.T01:CDS
AACACAGCAGAGAAGGTTCC+AGG	0.405306	4:+83415670	MsG0480023292.01.T01:CDS
AAGAATAAGGGGCAATCCTT+CGG	0.498704	4:-83416372	None:intergenic
AAGACAGGAAGCTGTTAGTA+TGG	0.418992	4:+83411980	MsG0480023292.01.T01:CDS
AAGCTGCTGTGAGTTAGAGA+GGG	0.654500	4:+83416877	MsG0480023292.01.T01:exon
AAGGCAGCAAACTTATTGCC+TGG	0.507560	4:+83416502	MsG0480023292.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTCAACACGGAGAAAT+AGG	+	Chr4:83414309-83414328	MsG0480023292.01.T01:intron	30.0%
AAAGAGGGGATAAAGAAAAA+TGG	-	Chr4:83414385-83414404	None:intergenic	30.0%
AATTCTGACATTCAATCAGT+TGG	+	Chr4:83414897-83414916	MsG0480023292.01.T01:intron	30.0%
AATTGGAAATATCACAAGAC+AGG	+	Chr4:83411965-83411984	MsG0480023292.01.T01:CDS	30.0%
AATTGTTGATCCACAAGAAT+GGG	+	Chr4:83410395-83410414	MsG0480023292.01.T01:CDS	30.0%
ACATTGACATATCTGTTAAG+CGG	+	Chr4:83416590-83416609	MsG0480023292.01.T01:intron	30.0%
ACGTAAATCTAACCTGATAA+TGG	+	Chr4:83413497-83413516	MsG0480023292.01.T01:intron	30.0%
AGACATACCATGTAGAAATA+AGG	-	Chr4:83411546-83411565	None:intergenic	30.0%
ATCTTCCAAATCATCTGTAT+GGG	-	Chr4:83415590-83415609	None:intergenic	30.0%
ATGTCAATGTCAATGTCAAT+GGG	-	Chr4:83416580-83416599	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	83410164	83417231	83410164	ID=MsG0480023292.01;Name=MsG0480023292.01
Chr4	mRNA	83410164	83417231	83410164	ID=MsG0480023292.01.T01;Parent=MsG0480023292.01;Name=MsG0480023292.01.T01;_AED=0.40;_eAED=0.40;_QI=49\|0.78\|0.73\|1\|1\|1\|15\|338\|662
Chr4	exon	83410164	83410475	83410164	ID=MsG0480023292.01.T01:exon:1090;Parent=MsG0480023292.01.T01
Chr4	exon	83410566	83410632	83410566	ID=MsG0480023292.01.T01:exon:1091;Parent=MsG0480023292.01.T01
Chr4	exon	83410715	83410830	83410715	ID=MsG0480023292.01.T01:exon:1092;Parent=MsG0480023292.01.T01
Chr4	exon	83411935	83412001	83411935	ID=MsG0480023292.01.T01:exon:1093;Parent=MsG0480023292.01.T01
Chr4	exon	83412379	83412427	83412379	ID=MsG0480023292.01.T01:exon:1094;Parent=MsG0480023292.01.T01
Chr4	exon	83412543	83412628	83412543	ID=MsG0480023292.01.T01:exon:1095;Parent=MsG0480023292.01.T01
Chr4	exon	83412771	83412801	83412771	ID=MsG0480023292.01.T01:exon:1096;Parent=MsG0480023292.01.T01
Chr4	exon	83412897	83413020	83412897	ID=MsG0480023292.01.T01:exon:1097;Parent=MsG0480023292.01.T01
Chr4	exon	83413098	83413153	83413098	ID=MsG0480023292.01.T01:exon:1098;Parent=MsG0480023292.01.T01
Chr4	exon	83414035	83414120	83414035	ID=MsG0480023292.01.T01:exon:1099;Parent=MsG0480023292.01.T01
Chr4	exon	83414418	83414510	83414418	ID=MsG0480023292.01.T01:exon:1100;Parent=MsG0480023292.01.T01
Chr4	exon	83415083	83415308	83415083	ID=MsG0480023292.01.T01:exon:1101;Parent=MsG0480023292.01.T01
Chr4	exon	83415464	83415691	83415464	ID=MsG0480023292.01.T01:exon:1102;Parent=MsG0480023292.01.T01
Chr4	exon	83416317	83416550	83416317	ID=MsG0480023292.01.T01:exon:1103;Parent=MsG0480023292.01.T01
Chr4	exon	83416631	83417231	83416631	ID=MsG0480023292.01.T01:exon:1104;Parent=MsG0480023292.01.T01
Chr4	five_prime_UTR	83410164	83410212	83410164	ID=MsG0480023292.01.T01:five_prime_utr;Parent=MsG0480023292.01.T01
Chr4	CDS	83410213	83410475	83410213	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83410566	83410632	83410566	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83410715	83410830	83410715	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83411935	83412001	83411935	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83412379	83412427	83412379	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83412543	83412628	83412543	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83412771	83412801	83412771	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83412897	83413020	83412897	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83413098	83413153	83413098	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83414035	83414120	83414035	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83414418	83414510	83414418	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83415083	83415308	83415083	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83415464	83415691	83415464	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83416317	83416550	83416317	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	CDS	83416631	83416893	83416631	ID=MsG0480023292.01.T01:cds;Parent=MsG0480023292.01.T01
Chr4	three_prime_UTR	83416894	83417231	83416894	ID=MsG0480023292.01.T01:three_prime_utr;Parent=MsG0480023292.01.T01

Gene Sequence

>MsG0480023292.01.T01

ATGGGTAGAGGAAGGTCACGAACTCAAAGAAAGGACTTTCGTCAAAACAGAGAAAACGTTTGGAAGCGTTCGAAACCAGATCCAGACCCTTCCTTATCCTCCGAAAATTCTCAAACTACTACTCATTGGACTCCTTTCTTGACTCAAAACCCTTCCTTTGATTCTTATTACAAGGAACAGTTAATTGTTGATCCACAAGAATGGGATCAATTCATCGCAGTTCTCCGAACACCATTGCCTGCATCATTTAGAATCAATTCTAGTAGTCAATTTGCTGATGATATTCGTTCCCAATTGGAAAATGATTTCGTGCATTCACTTCGCGCTGAGGTTGCTGAAGGAGGTGAGACAGAGGCGATTCGGCCTTTGCCTTGGTATCCCGGGAACTTTGCTTGGCATTCAAATTTTTCGCGGATGCAGCTTAGGAAGAATCAAACATTGGAGAGGTTCCACGAATTTTTAAAGCTAGAAAATGAAATTGGAAATATCACAAGACAGGAAGCTGTTAGTATGGTTCCTCCCCTATTCTTGGATGTGCATTCAGACCATTTTGTACTTGACATGTGTGCTGCACCAGGTTCAAAAACATTCCAATTGCTCGAGACTATACACCAATCCACAAAAGCAGGATCACTGCCTGATGGAATGGTTGTAGCAAATGATCTTGATGTTAAAAGATGGAATTATGAAAGAATGGAGTTAGATCACAATATTCACCAACTGTTATTTGATCGTGTTCTGTGCGATGTCCCGTGTAGTGGAGATGGTACACTTCGTAAGGCACCTGATATGTGGCGGAAATGGAATTCAGGGATGGGACAAGGGCTTCACAGCCTACAAGTTTTAATTGCTATGCGAGGTTTATCTTTACTAAAAATCGGGGGAAGAATGGTTTATTCAACGTGCTCAATGAATCCTATTGAAAATGAAGCTGTGGTTGCAGAGGTTTTGCGGAGGTGTGGTGAGTCTGTCAAACTTGTTGACGTGTCTAGTGAGCTTCCACAACTTATTCGTCGGCCAGGTCTCAAGAAATGGAAGGATCTTGGCGATAGTAATTGTAATGTTGTCATGGAAGATGACATTACTAGTGTGGAAAATAAAAAAACTGAAGATGGTATTGAAGCACTAGAGAATCCTGTGATGGCGGAGTCTGCTGAGGAAGTTTCAGATTTTCCTCTAGAGCATTGCATGAGGATAATGCCTCATGATCAGAACACTGGAGCCTTCTTCATTGCTGTGCTGCAAAAAGTTTCTCCTCTGCCAGCTATTCTGGAAAAACCTAGCAAACAGAGTCTTGAGGATGCACAAGCACCACACATGAATTCATCTGAAACTACACTTGAAGTCGTCAAAGCAGTTCCAGAGGAAAATGTGAATGATAATGTATCCCATACAGATGATTTGGAAGATAGTCCTCTAACACGTGAAGAACGGAATTATGAGGAAACTGAAGAGCCGCATAATGCACAAAACACAGCAGAGAAGGTTCCAGGAAAGACAAACAGCACGAGAAGGTGGCTCTTCACCATGTGCTTTCCGGATATCATCCGAAGGATTGCCCCTTATTCTTCCATACATAACCAAGCAAATTATACAGGCATCTCCCGAGGACTTCAAGCGTCTTCTGCAGGACAAAGATGTAAAATATTCAGATTTGCTGATGCCGAGTTTGGTGAAAAGGCAGCAAACTTATTGCCTGGTTGTTGCGTGGTTATTATGGGTAAAGGGAATACAGTTGCATCAGAATCCCTCAAAGTTGACGAGTCAACAATAGCAATCGGATGCTGGAGGGGTAGGGCGAGGTTGTCCGTGATGGTCTCTGCAATGGACTGCCAAGAATTGCTTGCAAGACTTTTAATACGTCTTGGCACAGAAAATGGCTCTTCTGGGCAGGTGGACAATTCCTCTAACTATGTTGGAGGTGAAGCACAGGCTGTACAGGAGTTGAACGATAAAAATGATAATGATTTGAAAGCTGCTGTGAGTTAG

Protein sequence

>MsG0480023292.01.T01

MGRGRSRTQRKDFRQNRENVWKRSKPDPDPSLSSENSQTTTHWTPFLTQNPSFDSYYKEQLIVDPQEWDQFIAVLRTPLPASFRINSSSQFADDIRSQLENDFVHSLRAEVAEGGETEAIRPLPWYPGNFAWHSNFSRMQLRKNQTLERFHEFLKLENEIGNITRQEAVSMVPPLFLDVHSDHFVLDMCAAPGSKTFQLLETIHQSTKAGSLPDGMVVANDLDVKRWNYERMELDHNIHQLLFDRVLCDVPCSGDGTLRKAPDMWRKWNSGMGQGLHSLQVLIAMRGLSLLKIGGRMVYSTCSMNPIENEAVVAEVLRRCGESVKLVDVSSELPQLIRRPGLKKWKDLGDSNCNVVMEDDITSVENKKTEDGIEALENPVMAESAEEVSDFPLEHCMRIMPHDQNTGAFFIAVLQKVSPLPAILEKPSKQSLEDAQAPHMNSSETTLEVVKAVPEENVNDNVSHTDDLEDSPLTREERNYEETEEPHNAQNTAEKVPGKTNSTRRWLFTMCFPDIIRRIAPYSSIHNQANYTGISRGLQASSAGQRCKIFRFADAEFGEKAANLLPGCCVVIMGKGNTVASESLKVDESTIAIGCWRGRARLSVMVSAMDCQELLARLLIRLGTENGSSGQVDNSSNYVGGEAQAVQELNDKNDNDLKAAVS*