AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023516.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023516.01.T01 MTR_4g134740 64.384 73 26 0 110 182 43 115 6.88e-26 96.3
MsG0480023516.01.T01 MTR_4g134740 59.574 47 19 0 19 65 72 118 4.15e-13 63.2
MsG0480023516.01.T01 MTR_6g069780 78.947 57 12 0 126 182 35 91 4.41e-25 93.6
MsG0480023516.01.T01 MTR_6g069780 79.592 49 10 0 17 65 46 94 1.20e-21 84.7
MsG0480023516.01.T01 MTR_7g018160 55.172 87 38 1 97 182 206 292 1.23e-23 94.7
MsG0480023516.01.T01 MTR_7g018160 56.522 46 20 0 17 62 247 292 9.92e-12 62.4
MsG0480023516.01.T01 MTR_6g018340 64.615 65 23 0 118 182 1 65 1.66e-23 88.6
MsG0480023516.01.T01 MTR_6g018340 62.500 48 18 0 18 65 21 68 4.62e-16 69.3
MsG0480023516.01.T01 MTR_7g108380 75.000 60 14 1 124 182 19 78 2.12e-23 88.6
MsG0480023516.01.T01 MTR_7g108380 60.000 50 20 0 13 62 29 78 2.24e-15 68.2
MsG0480023516.01.T01 MTR_7g046285 62.500 72 27 0 111 182 71 142 2.98e-23 90.5
MsG0480023516.01.T01 MTR_7g046285 64.583 48 17 0 18 65 98 145 5.36e-14 66.2
MsG0480023516.01.T01 MTR_3g100990 57.471 87 30 1 103 182 416 502 4.60e-23 95.5
MsG0480023516.01.T01 MTR_3g100990 64.151 53 19 0 13 65 453 505 9.29e-17 77.4
MsG0480023516.01.T01 MTR_3g109050 62.500 72 27 0 111 182 148 219 1.12e-22 90.9
MsG0480023516.01.T01 MTR_3g109050 59.184 49 20 0 17 65 174 222 3.66e-12 62.8
MsG0480023516.01.T01 MTR_1g018670 63.889 72 24 1 111 182 27 96 2.84e-22 89.0
MsG0480023516.01.T01 MTR_1g018670 57.447 47 18 1 18 64 54 98 3.38e-11 59.7
MsG0480023516.01.T01 MTR_5g071700 83.673 49 8 0 17 65 376 424 1.29e-21 90.9
MsG0480023516.01.T01 MTR_5g071700 69.231 52 16 0 131 182 370 421 1.23e-17 79.7
MsG0480023516.01.T01 MTR_8g039470 82.979 47 8 0 19 65 48 94 2.24e-21 84.0
MsG0480023516.01.T01 MTR_8g039470 68.182 44 14 0 139 182 48 91 2.29e-14 65.9
MsG0480023516.01.T01 MTR_6g027930 60.000 65 26 0 118 182 1 65 1.80e-20 81.6
MsG0480023516.01.T01 MTR_6g027930 55.319 47 21 0 18 64 21 67 3.32e-12 60.5
MsG0480023516.01.T01 MTR_0007s0160 63.077 65 24 0 118 182 1 65 2.30e-20 81.3
MsG0480023516.01.T01 MTR_0007s0160 63.043 46 17 0 17 62 20 65 8.48e-11 56.2
MsG0480023516.01.T01 MTR_5g064790 75.510 49 12 0 17 65 76 124 2.57e-19 79.7
MsG0480023516.01.T01 MTR_5g064790 60.656 61 24 0 122 182 61 121 1.11e-17 75.5
MsG0480023516.01.T01 MTR_2g054670 78.723 47 10 0 19 65 99 145 5.76e-19 79.3
MsG0480023516.01.T01 MTR_2g054670 56.364 55 24 0 128 182 88 142 1.43e-14 67.8
MsG0480023516.01.T01 MTR_0017s0290 68.421 57 18 0 126 182 356 412 2.59e-18 81.6
MsG0480023516.01.T01 MTR_0017s0290 65.306 49 17 0 17 65 367 415 3.50e-15 72.8
MsG0480023516.01.T01 MTR_5g464580 61.404 57 22 0 126 182 37 93 6.04e-18 75.1
MsG0480023516.01.T01 MTR_5g464580 60.870 46 18 0 17 62 48 93 1.80e-14 66.2
MsG0480023516.01.T01 MTR_4g062370 65.574 61 20 1 123 182 665 725 7.53e-18 80.9
MsG0480023516.01.T01 MTR_4g062370 65.574 61 20 1 5 65 669 728 9.40e-18 80.5
MsG0480023516.01.T01 MTR_8g072100 55.844 77 27 1 106 182 117 186 1.02e-17 77.0
MsG0480023516.01.T01 MTR_8g072100 64.583 48 17 0 18 65 142 189 1.28e-13 66.2
MsG0480023516.01.T01 MTR_3g452260 66.667 48 16 0 18 65 52 99 1.06e-17 74.7
MsG0480023516.01.T01 MTR_3g452260 60.345 58 23 0 125 182 39 96 1.85e-17 73.9
MsG0480023516.01.T01 MTR_3g451160 66.667 48 16 0 18 65 52 99 1.06e-17 74.7
MsG0480023516.01.T01 MTR_3g451160 60.345 58 23 0 125 182 39 96 1.85e-17 73.9
MsG0480023516.01.T01 MTR_4g009693 65.455 55 19 0 128 182 42 96 2.03e-17 73.9
MsG0480023516.01.T01 MTR_4g009693 54.098 61 27 1 5 65 40 99 2.23e-13 63.5
MsG0480023516.01.T01 MTR_3g048960 31.720 186 73 5 18 149 70 255 4.20e-17 76.6
MsG0480023516.01.T01 MTR_3g048960 58.065 62 26 0 121 182 53 114 8.17e-12 62.4
MsG0480023516.01.T01 MTR_5g009440 58.730 63 26 0 120 182 106 168 4.99e-17 74.7
MsG0480023516.01.T01 MTR_5g009440 57.143 49 21 0 17 65 123 171 6.11e-12 61.2
MsG0480023516.01.T01 MTR_3g050180 58.929 56 22 1 10 65 12 66 5.88e-17 71.6
MsG0480023516.01.T01 MTR_5g087830 52.778 72 34 0 111 182 182 253 1.41e-16 75.5
MsG0480023516.01.T01 MTR_5g087830 65.957 47 16 0 19 65 210 256 7.97e-14 67.8
MsG0480023516.01.T01 MTR_3g101720 78.049 41 9 0 109 149 19 59 1.67e-16 70.5
MsG0480023516.01.T01 MTR_8g096220 61.111 54 21 0 12 65 82 135 2.23e-16 72.4
MsG0480023516.01.T01 MTR_8g096220 58.696 46 19 0 137 182 87 132 6.53e-11 57.8
MsG0480023516.01.T01 MTR_1g098830 58.108 74 24 4 110 178 6 77 3.59e-16 70.1
MsG0480023516.01.T01 MTR_3g082460 83.721 43 7 0 140 182 257 299 3.64e-16 74.7
MsG0480023516.01.T01 MTR_3g082460 69.565 46 14 0 20 65 257 302 9.66e-15 70.9
MsG0480023516.01.T01 MTR_6g029550 59.016 61 25 0 122 182 333 393 6.51e-16 74.7
MsG0480023516.01.T01 MTR_6g029550 59.184 49 20 0 17 65 348 396 3.45e-12 64.3
MsG0480023516.01.T01 MTR_3g010480 64.151 53 19 0 130 182 6 58 1.06e-15 68.6
MsG0480023516.01.T01 MTR_3g010480 62.500 48 18 0 18 65 14 61 6.43e-15 66.2
MsG0480023516.01.T01 MTR_5g061500 61.667 60 22 1 124 182 432 491 1.36e-15 73.9
MsG0480023516.01.T01 MTR_5g061500 65.306 49 17 0 17 65 446 494 5.15e-15 72.4
MsG0480023516.01.T01 MTR_0786s0010 68.085 47 14 1 19 65 13 58 1.38e-15 68.2
MsG0480023516.01.T01 MTR_0786s0010 58.929 56 22 1 127 182 1 55 4.02e-14 64.3
MsG0480023516.01.T01 MTR_7g110460 51.948 77 34 1 104 180 92 165 1.42e-15 71.6
MsG0480023516.01.T01 MTR_7g110460 69.767 43 13 0 18 60 123 165 5.02e-13 65.1
MsG0480023516.01.T01 MTR_8g057780 54.098 61 28 0 5 65 61 121 2.31e-15 69.3
MsG0480023516.01.T01 MTR_8g057780 53.704 54 25 0 129 182 65 118 3.88e-13 63.5
MsG0480023516.01.T01 MTR_3g047930 63.830 47 17 0 19 65 111 157 6.05e-15 68.9
MsG0480023516.01.T01 MTR_3g047930 65.909 44 15 0 139 182 111 154 2.87e-11 59.3
MsG0480023516.01.T01 MTR_4g009520 64.583 48 17 0 18 65 154 201 7.30e-15 69.7
MsG0480023516.01.T01 MTR_4g009520 65.909 44 15 0 139 182 155 198 4.78e-11 59.3
MsG0480023516.01.T01 MTR_7g060880 52.174 92 21 1 114 182 23 114 7.62e-15 68.9
MsG0480023516.01.T01 MTR_7g060880 68.750 48 15 0 18 65 70 117 1.19e-12 62.8
MsG0480023516.01.T01 MTR_1g045920 64.151 53 19 0 13 65 220 272 7.95e-15 70.9
MsG0480023516.01.T01 MTR_1g045920 40.404 99 47 1 96 182 171 269 9.65e-14 67.8
MsG0480023516.01.T01 MTR_4g010800 71.739 46 13 0 111 156 119 164 8.18e-15 68.9
MsG0480023516.01.T01 MTR_3g114680 80.488 41 8 0 25 65 102 142 1.19e-14 67.8
MsG0480023516.01.T01 MTR_2g100050 53.226 62 29 0 5 66 72 133 1.19e-14 67.8
MsG0480023516.01.T01 MTR_2g100050 58.824 51 21 0 132 182 79 129 6.60e-12 60.5
MsG0480023516.01.T01 MTR_3g101850 64.583 48 17 0 18 65 177 224 1.21e-14 69.7
MsG0480023516.01.T01 MTR_1g047970 71.739 46 13 0 111 156 182 227 1.31e-14 71.6
MsG0480023516.01.T01 MTR_3g085980 58.065 62 25 1 122 182 130 191 1.37e-14 68.9
MsG0480023516.01.T01 MTR_3g085980 65.909 44 15 0 22 65 151 194 9.88e-14 66.6
MsG0480023516.01.T01 MTR_3g072880 59.649 57 23 0 114 170 50 106 1.84e-14 67.4
MsG0480023516.01.T01 MTR_1g106700 61.224 49 19 0 17 65 186 234 1.88e-14 69.3
MsG0480023516.01.T01 MTR_1g106700 58.491 53 22 0 130 182 179 231 3.16e-14 68.6
MsG0480023516.01.T01 MTR_6g044370 64.706 51 18 0 132 182 251 301 1.95e-14 70.1
MsG0480023516.01.T01 MTR_6g044370 63.265 49 18 0 17 65 256 304 2.82e-14 69.7
MsG0480023516.01.T01 MTR_1g107470 69.767 43 13 0 111 153 330 372 2.10e-14 70.5
MsG0480023516.01.T01 MTR_0390s0030 68.085 47 14 1 19 65 54 99 2.16e-14 65.9
MsG0480023516.01.T01 MTR_0390s0030 61.404 57 21 1 126 182 41 96 2.54e-14 65.9
MsG0480023516.01.T01 MTR_7g022020 55.738 61 27 0 122 182 542 602 2.92e-14 70.5
MsG0480023516.01.T01 MTR_7g022020 59.184 49 20 0 17 65 557 605 1.09e-13 68.6
MsG0480023516.01.T01 MTR_8g039580 55.932 59 26 0 124 182 102 160 5.62e-14 66.6
MsG0480023516.01.T01 MTR_8g039580 55.102 49 22 0 17 65 115 163 2.83e-11 59.3
MsG0480023516.01.T01 MTR_6g073050 50.704 71 29 1 104 174 25 89 5.95e-14 65.1
MsG0480023516.01.T01 MTR_8g016050 58.462 65 24 1 118 182 1 62 6.16e-14 64.3
MsG0480023516.01.T01 MTR_8g016050 54.386 57 26 0 8 64 8 64 7.14e-13 61.6
MsG0480023516.01.T01 MTR_6g013370 66.667 45 15 0 126 170 10 54 6.33e-14 63.5
MsG0480023516.01.T01 MTR_0196s0030 57.692 52 22 0 129 180 86 137 1.97e-13 67.4
MsG0480023516.01.T01 MTR_0196s0030 66.667 42 14 0 19 60 96 137 3.80e-11 60.8
MsG0480023516.01.T01 MTR_1g012040 51.429 70 34 0 113 182 410 479 2.02e-13 67.8
MsG0480023516.01.T01 MTR_1g012040 53.571 56 26 0 17 72 434 489 1.05e-12 65.9
MsG0480023516.01.T01 MTR_2g058900 65.217 46 16 0 111 156 84 129 2.27e-13 64.3
MsG0480023516.01.T01 MTR_4g121520 75.610 41 10 0 18 58 157 197 2.32e-13 67.8
MsG0480023516.01.T01 MTR_8g466670 63.265 49 18 0 12 60 120 168 2.35e-13 65.5
MsG0480023516.01.T01 MTR_0025s0050 64.444 45 16 0 110 154 155 199 3.40e-13 65.1
MsG0480023516.01.T01 MTR_4g030760 56.923 65 25 1 118 182 1 62 3.43e-13 67.0
MsG0480023516.01.T01 MTR_4g030760 58.696 46 19 0 18 63 18 63 3.19e-11 61.2
MsG0480023516.01.T01 MTR_8g073690 54.167 48 22 0 18 65 23 70 3.98e-13 62.0
MsG0480023516.01.T01 MTR_8g036260 61.702 47 18 0 19 65 316 362 4.35e-13 66.6
MsG0480023516.01.T01 MTR_1g101230 61.290 62 24 0 121 182 113 174 4.42e-13 64.7
MsG0480023516.01.T01 MTR_1g101230 66.667 48 16 0 18 65 130 177 5.17e-12 61.6
MsG0480023516.01.T01 MTR_2g064230 61.224 49 19 0 18 66 381 429 5.02e-13 66.6
MsG0480023516.01.T01 MTR_3g023450 47.761 67 30 1 111 172 42 108 5.95e-13 62.8
MsG0480023516.01.T01 MTR_8g085330 52.778 72 17 1 111 182 215 269 5.97e-13 65.5
MsG0480023516.01.T01 MTR_6g008980 40.426 94 49 2 86 172 5 98 6.42e-13 63.2
MsG0480023516.01.T01 MTR_2g008505 62.295 61 23 0 122 182 288 348 6.49e-13 66.2
MsG0480023516.01.T01 MTR_2g008505 66.667 48 16 0 18 65 304 351 1.38e-11 62.4
MsG0480023516.01.T01 MTR_3g114250 46.479 71 36 1 114 182 17 87 9.23e-13 64.3
MsG0480023516.01.T01 MTR_3g032450 45.205 73 26 1 110 182 19 77 9.23e-13 61.2
MsG0480023516.01.T01 MTR_1g081340 44.872 78 34 1 105 182 338 406 9.97e-13 65.9
MsG0480023516.01.T01 MTR_1g081340 60.870 46 18 0 17 62 361 406 4.54e-12 63.9
MsG0480023516.01.T01 MTR_5g034510 48.611 72 21 1 111 182 33 88 1.18e-12 61.2
MsG0480023516.01.T01 MTR_8g057880 59.677 62 25 0 121 182 142 203 1.32e-12 64.7
MsG0480023516.01.T01 MTR_7g006890 62.500 48 18 0 132 179 28 75 1.89e-12 63.5
MsG0480023516.01.T01 MTR_7g006890 38.776 98 51 2 6 98 17 110 1.28e-11 61.2
MsG0480023516.01.T01 MTR_0073s0030 65.116 43 15 0 111 153 164 206 2.20e-12 63.2
MsG0480023516.01.T01 MTR_8g075630 65.116 43 15 0 111 153 164 206 2.29e-12 63.2
MsG0480023516.01.T01 MTR_7g113060 70.732 41 12 0 111 151 150 190 2.67e-12 62.8
MsG0480023516.01.T01 MTR_5g071690 73.171 41 11 0 127 167 93 133 2.72e-12 61.6
MsG0480023516.01.T01 MTR_1g093010 48.485 66 34 0 111 176 287 352 3.01e-12 64.3
MsG0480023516.01.T01 MTR_5g080630 77.143 35 8 0 31 65 108 142 4.30e-12 61.2
MsG0480023516.01.T01 MTR_8g022960 76.923 39 9 0 27 65 228 266 6.09e-12 62.8
MsG0480023516.01.T01 MTR_4g096800 56.863 51 22 0 12 62 222 272 7.72e-12 62.8
MsG0480023516.01.T01 MTR_4g014550 59.091 44 18 0 22 65 35 78 1.33e-11 58.5
MsG0480023516.01.T01 MTR_4g076760 65.854 41 14 0 111 151 486 526 1.43e-11 62.4
MsG0480023516.01.T01 MTR_7g052070 59.574 47 19 0 103 149 181 227 1.47e-11 61.2
MsG0480023516.01.T01 MTR_3g095720 47.059 68 27 1 11 78 69 127 2.01e-11 59.3
MsG0480023516.01.T01 MTR_0134s0020 58.000 50 21 0 133 182 299 348 2.09e-11 61.6
MsG0480023516.01.T01 MTR_0014s0220 54.386 57 24 1 110 164 573 629 2.24e-11 62.0
MsG0480023516.01.T01 MTR_3g052540 50.943 53 26 0 13 65 236 288 2.34e-11 61.2
MsG0480023516.01.T01 MTR_8g023250 49.057 53 27 0 13 65 261 313 2.58e-11 61.2
MsG0480023516.01.T01 MTR_4g039550 37.113 97 61 0 86 182 85 181 2.62e-11 60.1
MsG0480023516.01.T01 MTR_2g065670 56.452 62 20 3 124 180 276 335 2.70e-11 61.6
MsG0480023516.01.T01 MTR_8g015900 52.632 57 21 1 115 165 8 64 3.37e-11 57.0
MsG0480023516.01.T01 MTR_8g056080 45.455 66 31 1 112 172 11 76 3.42e-11 57.4
MsG0480023516.01.T01 MTR_6g086725 56.818 44 19 0 18 61 9 52 3.42e-11 58.5
MsG0480023516.01.T01 MTR_6g012610 53.846 52 17 1 103 147 51 102 3.60e-11 57.8
MsG0480023516.01.T01 MTR_1g093700 50.943 53 26 0 13 65 265 317 3.99e-11 60.8
MsG0480023516.01.T01 MTR_7g498270 67.391 46 8 1 110 155 70 108 5.06e-11 57.4
MsG0480023516.01.T01 MTR_3g110165 59.091 44 18 0 112 155 24 67 5.32e-11 56.2
MsG0480023516.01.T01 MTR_3g051520 70.270 37 10 1 132 168 5 40 6.65e-11 56.2
MsG0480023516.01.T01 MTR_6g086070 36.607 112 61 3 73 182 42 145 7.52e-11 57.8
MsG0480023516.01.T01 MTR_1g092930 46.970 66 35 0 111 176 309 374 7.99e-11 60.1
MsG0480023516.01.T01 MTR_8g008750 57.143 49 19 1 17 65 39 85 9.31e-11 56.2
MsG0480023516.01.T01 MTR_4g034880 50.000 48 24 0 20 67 327 374 9.84e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023516.01.T01 AT3G51700 40.000 105 56 3 80 182 233 332 4.29e-17 77.8
MsG0480023516.01.T01 AT3G51690 45.205 73 39 1 111 182 256 328 1.77e-15 73.2
MsG0480023516.01.T01 AT5G28780 50.000 72 34 1 111 182 258 327 6.91e-12 63.2

Find 29 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCAAAACAAACGGTAAT+AGG 0.280297 4:-86356871 None:intergenic
TGGAAACTGCGACATATAAT+TGG 0.304950 4:-86356969 None:intergenic
TTGGAACTTGAAGGGAATTC+TGG 0.305895 4:-86356838 None:intergenic
ATACTCATAATTGATGAAAA+TGG 0.309546 4:+86357018 MsG0480023516.01.T01:CDS
AATTATATGTCGCAGTTTCC+AGG 0.364380 4:+86356971 MsG0480023516.01.T01:CDS
GCTTCACCCGTGCTGCCGCA+CGG 0.431358 4:-86356763 None:intergenic
CTTCACCCGTGCTGCCGCAC+GGG 0.444248 4:-86356762 None:intergenic
ATGCCTACATTGGAACTTGA+AGG 0.466681 4:-86356847 None:intergenic
CAGCACGGGTGAAGCAGCAT+TGG 0.472658 4:+86356771 MsG0480023516.01.T01:CDS
TACTTTATTGTATTCGACAT+TGG 0.492584 4:-86357061 None:intergenic
TCAAGAGTGACATCGAGAAA+TGG 0.498682 4:+86356524 MsG0480023516.01.T01:CDS
TATGACCCGTGCGGCAGCAC+GGG 0.513644 4:+86356757 MsG0480023516.01.T01:CDS
AGGCAATCATTAAAACAAGT+TGG 0.519061 4:+86356913 MsG0480023516.01.T01:CDS
CAGTTTCCAGGGTTACATCA+AGG 0.524089 4:+86356983 MsG0480023516.01.T01:CDS
CATGAAAACTACATTAAATG+TGG 0.533411 4:+86356583 MsG0480023516.01.T01:CDS
TTCCCTTCAAGTTCCAATGT+AGG 0.537083 4:+86356844 MsG0480023516.01.T01:CDS
AAGGGAATTCTGGTGTCTGA+AGG 0.542038 4:-86356828 None:intergenic
AATGGTCATTGCAAAACAAA+CGG 0.543125 4:-86356878 None:intergenic
AACTTTCCTTGATGTAACCC+TGG 0.545322 4:-86356989 None:intergenic
GGGCAACCAGTCTTCTCTCA+TGG 0.547963 4:+86356482 MsG0480023516.01.T01:CDS
GGTAATAGGAATGCCTACAT+TGG 0.566041 4:-86356857 None:intergenic
ATTATATGTCGCAGTTTCCA+GGG 0.569703 4:+86356972 MsG0480023516.01.T01:CDS
AGCTGACCATGAGAGAAGAC+TGG 0.595908 4:-86356488 None:intergenic
ATATGACCCGTGCGGCAGCA+CGG 0.597094 4:+86356756 MsG0480023516.01.T01:CDS
TGCCTACATTGGAACTTGAA+GGG 0.613171 4:-86356846 None:intergenic
GAGAGAATACAGACTGTGGT+AGG 0.634563 4:-86356942 None:intergenic
TGACCATTAACAAAAGTCGA+AGG 0.665643 4:+86356893 MsG0480023516.01.T01:CDS
TTGTGAGAGAATACAGACTG+TGG 0.791691 4:-86356946 None:intergenic
AAAGATGTATATGACCCGTG+CGG 0.796117 4:+86356748 MsG0480023516.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACTCATAATTGATGAAAA+TGG + Chr4:86357018-86357037 MsG0480023516.01.T01:CDS 20.0%
!!! AAGTAAAACTTTTATTTGGA+GGG + Chr4:86356462-86356481 MsG0480023516.01.T01:CDS 20.0%
!!! ATGTGTTGTCTATTTTTATT+GGG + Chr4:86356437-86356456 MsG0480023516.01.T01:CDS 20.0%
! ATTATAATCATAAGCGATGA+TGG + Chr4:86356696-86356715 MsG0480023516.01.T01:intron 25.0%
! CATGAAAACTACATTAAATG+TGG + Chr4:86356583-86356602 MsG0480023516.01.T01:CDS 25.0%
! TACTTTATTGTATTCGACAT+TGG - Chr4:86357064-86357083 None:intergenic 25.0%
!! ATTTTGTTGACAGATGAAAA+TGG + Chr4:86356554-86356573 MsG0480023516.01.T01:CDS 25.0%
!! GGACAAGTAAAACTTTTATT+TGG + Chr4:86356458-86356477 MsG0480023516.01.T01:CDS 25.0%
!!! CAAGTAAAACTTTTATTTGG+AGG + Chr4:86356461-86356480 MsG0480023516.01.T01:CDS 25.0%
AATGGTCATTGCAAAACAAA+CGG - Chr4:86356881-86356900 None:intergenic 30.0%
AGGCAATCATTAAAACAAGT+TGG + Chr4:86356913-86356932 MsG0480023516.01.T01:CDS 30.0%
ATTGCAAAACAAACGGTAAT+AGG - Chr4:86356874-86356893 None:intergenic 30.0%
AATTATATGTCGCAGTTTCC+AGG + Chr4:86356971-86356990 MsG0480023516.01.T01:CDS 35.0%
ATTATATGTCGCAGTTTCCA+GGG + Chr4:86356972-86356991 MsG0480023516.01.T01:CDS 35.0%
TGACCATTAACAAAAGTCGA+AGG + Chr4:86356893-86356912 MsG0480023516.01.T01:CDS 35.0%
TGGAAACTGCGACATATAAT+TGG - Chr4:86356972-86356991 None:intergenic 35.0%
! TTGCCTTCGACTTTTGTTAA+TGG - Chr4:86356899-86356918 None:intergenic 35.0%
AAAGATGTATATGACCCGTG+CGG + Chr4:86356748-86356767 MsG0480023516.01.T01:CDS 40.0%
AACTTTCCTTGATGTAACCC+TGG - Chr4:86356992-86357011 None:intergenic 40.0%
ATGCCTACATTGGAACTTGA+AGG - Chr4:86356850-86356869 None:intergenic 40.0%
GGTAATAGGAATGCCTACAT+TGG - Chr4:86356860-86356879 None:intergenic 40.0%
TCAAGAGTGACATCGAGAAA+TGG + Chr4:86356524-86356543 MsG0480023516.01.T01:CDS 40.0%
TGCCTACATTGGAACTTGAA+GGG - Chr4:86356849-86356868 None:intergenic 40.0%
TTGGAACTTGAAGGGAATTC+TGG - Chr4:86356841-86356860 None:intergenic 40.0%
TTGTGAGAGAATACAGACTG+TGG - Chr4:86356949-86356968 None:intergenic 40.0%
! TTCCCTTCAAGTTCCAATGT+AGG + Chr4:86356844-86356863 MsG0480023516.01.T01:CDS 40.0%
AAGGGAATTCTGGTGTCTGA+AGG - Chr4:86356831-86356850 None:intergenic 45.0%
CAGTTTCCAGGGTTACATCA+AGG + Chr4:86356983-86357002 MsG0480023516.01.T01:CDS 45.0%
GAGAGAATACAGACTGTGGT+AGG - Chr4:86356945-86356964 None:intergenic 45.0%
AGCTGACCATGAGAGAAGAC+TGG - Chr4:86356491-86356510 None:intergenic 50.0%
GGGCAACCAGTCTTCTCTCA+TGG + Chr4:86356482-86356501 MsG0480023516.01.T01:CDS 55.0%
ATATGACCCGTGCGGCAGCA+CGG + Chr4:86356756-86356775 MsG0480023516.01.T01:CDS 60.0%
CAGCACGGGTGAAGCAGCAT+TGG + Chr4:86356771-86356790 MsG0480023516.01.T01:CDS 60.0%
TATGACCCGTGCGGCAGCAC+GGG + Chr4:86356757-86356776 MsG0480023516.01.T01:CDS 65.0%
CTTCACCCGTGCTGCCGCAC+GGG - Chr4:86356765-86356784 None:intergenic 70.0%
GCTTCACCCGTGCTGCCGCA+CGG - Chr4:86356766-86356785 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 86356437 86357089 86356437 ID=MsG0480023516.01;Name=MsG0480023516.01
Chr4 mRNA 86356437 86357089 86356437 ID=MsG0480023516.01.T01;Parent=MsG0480023516.01;Name=MsG0480023516.01.T01;_AED=0.49;_eAED=0.65;_QI=0|0|0|1|0|0|2|0|182
Chr4 exon 86356437 86356630 86356437 ID=MsG0480023516.01.T01:exon:1663;Parent=MsG0480023516.01.T01
Chr4 exon 86356735 86357089 86356735 ID=MsG0480023516.01.T01:exon:1664;Parent=MsG0480023516.01.T01
Chr4 CDS 86356437 86356630 86356437 ID=MsG0480023516.01.T01:cds;Parent=MsG0480023516.01.T01
Chr4 CDS 86356735 86357089 86356735 ID=MsG0480023516.01.T01:cds;Parent=MsG0480023516.01.T01
Gene Sequence

>MsG0480023516.01.T01

ATGTGTTGTCTATTTTTATTGGGACAAGTAAAACTTTTATTTGGAGGGCAACCAGTCTTCTCTCATGGTCAGCTATATGTGACGATTTCAAGAGTGACATCGAGAAATGGTCTTAAAATTTTGTTGACAGATGAAAATGGCGATTGCATGAAAACTACATTAAATGTGGTTTATCAAGAGATTTTTCAAAATGTTACAATATTTTGCAAAGATGTATATGACCCGTGCGGCAGCACGGGTGAAGCAGCATTGGTGACAAGATGTATGTGCATAGACTATCATTGCAACCTTCAGACACCAGAATTCCCTTCAAGTTCCAATGTAGGCATTCCTATTACCGTTTGTTTTGCAATGACCATTAACAAAAGTCGAAGGCAATCATTAAAACAAGTTGGTATCTACCTACCACAGTCTGTATTCTCTCACAACCAATTATATGTCGCAGTTTCCAGGGTTACATCAAGGAAAGTTTTGAAAATACTCATAATTGATGAAAATGGTGATTGCATTGATAACACAACCAATGTCGAATACAATAAAGTATTTTAG
Protein sequence

>MsG0480023516.01.T01

MCCLFLLGQVKLLFGGQPVFSHGQLYVTISRVTSRNGLKILLTDENGDCMKTTLNVVYQEIFQNVTIFCKDVYDPCGSTGEAALVTRCMCIDYHCNLQTPEFPSSSNVGIPITVCFAMTINKSRRQSLKQVGIYLPQSVFSHNQLYVAVSRVTSRKVLKILIIDENGDCIDNTTNVEYNKVF*