Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023709.01.T01 | XP_039687916.1 | 76.667 | 60 | 14 | 0 | 1 | 60 | 117 | 176 | 5.37E-19 | 85.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013883.01 | MsG0480023709.01 | 0.801188 | 1.012835e-48 | 4.806611e-46 |
| MsG0480023709.01 | MsG0580029381.01 | 0.802784 | 4.761075e-49 | 2.352923e-46 |
| MsG0280009306.01 | MsG0480023709.01 | 0.800206 | 1.606879e-48 | 7.438060e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023709.01.T01 | MTR_3g103470 | 50.000 | 60 | 30 | 0 | 1 | 60 | 31 | 90 | 2.92e-13 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGACCTCTTATCACTTGTTT+TGG | 0.262937 | 4:+88830200 | None:intergenic |
| CTCTAATAGCATTGACTTTG+TGG | 0.308814 | 4:-88830178 | MsG0480023709.01.T01:CDS |
| CACAAAGTCAATGCTATTAG+AGG | 0.387370 | 4:+88830179 | None:intergenic |
| CTGCATGAGTCCTCAGCCTC+TGG | 0.468451 | 4:+88830252 | None:intergenic |
| CCACCAAAACAAGTGATAAG+AGG | 0.531488 | 4:-88830203 | MsG0480023709.01.T01:CDS |
| TTGACACGAACAACTCTGGT+AGG | 0.549701 | 4:+88830114 | None:intergenic |
| GCAGGATGTACCAGAGGCTG+AGG | 0.587933 | 4:-88830262 | None:intergenic |
| TCGTGTCAAAATTGAGCACA+AGG | 0.614456 | 4:-88830100 | MsG0480023709.01.T01:CDS |
| ATGTCTGCAGGATGTACCAG+AGG | 0.658971 | 4:-88830268 | None:intergenic |
| TGATGAAGCTAACCAGACGT+CGG | 0.743571 | 4:-88830145 | MsG0480023709.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CACAAAGTCAATGCTATTAG+AGG | + | Chr4:88830162-88830181 | None:intergenic | 35.0% | |
| !! | AATTTTGACACGAACAACTC+TGG | + | Chr4:88830231-88830250 | None:intergenic | 35.0% |
| !! | CTCTAATAGCATTGACTTTG+TGG | - | Chr4:88830160-88830179 | MsG0480023709.01.T01:CDS | 35.0% |
| !!! | TTATCACTTGTTTTGGTGGT+TGG | + | Chr4:88830134-88830153 | None:intergenic | 35.0% |
| CAAGGACGACAAAGATGTTT+AGG | - | Chr4:88830256-88830275 | MsG0480023709.01.T01:CDS | 40.0% | |
| CCACCAAAACAAGTGATAAG+AGG | - | Chr4:88830135-88830154 | MsG0480023709.01.T01:CDS | 40.0% | |
| TCGTGTCAAAATTGAGCACA+AGG | - | Chr4:88830238-88830257 | MsG0480023709.01.T01:CDS | 40.0% | |
| ! | GGACCTCTTATCACTTGTTT+TGG | + | Chr4:88830141-88830160 | None:intergenic | 40.0% |
| !!! | CCTCTTATCACTTGTTTTGG+TGG | + | Chr4:88830138-88830157 | None:intergenic | 40.0% |
| TGATGAAGCTAACCAGACGT+CGG | - | Chr4:88830193-88830212 | MsG0480023709.01.T01:CDS | 45.0% | |
| TTGACACGAACAACTCTGGT+AGG | + | Chr4:88830227-88830246 | None:intergenic | 45.0% | |
| !! | TAGGTTTTGTGACCGACGTC+TGG | + | Chr4:88830208-88830227 | None:intergenic | 50.0% |
| CTGCATGAGTCCTCAGCCTC+TGG | + | Chr4:88830089-88830108 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 88830083 | 88830277 | 88830083 | ID=MsG0480023709.01;Name=MsG0480023709.01 |
| Chr4 | mRNA | 88830083 | 88830277 | 88830083 | ID=MsG0480023709.01.T01;Parent=MsG0480023709.01;Name=MsG0480023709.01.T01;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|0|1|0|64 |
| Chr4 | exon | 88830083 | 88830277 | 88830083 | ID=MsG0480023709.01.T01:exon:25143;Parent=MsG0480023709.01.T01 |
| Chr4 | CDS | 88830083 | 88830277 | 88830083 | ID=MsG0480023709.01.T01:cds;Parent=MsG0480023709.01.T01 |
| Gene Sequence |
| Protein sequence |
