AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023711.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023711.01.T01 MTR_8g099685 96.308 325 8 2 1 324 1 322 0.0 637
MsG0480023711.01.T01 MTR_8g099685 96.382 304 7 2 1 303 1 301 0.0 596
MsG0480023711.01.T01 MTR_3g081090 59.531 341 111 4 1 324 1 331 2.34e-131 377
MsG0480023711.01.T01 MTR_3g081090 59.248 319 103 4 1 302 1 309 1.39e-120 349
MsG0480023711.01.T01 MTR_7g029130 51.724 319 118 6 6 311 2 297 3.27e-98 292
MsG0480023711.01.T01 MTR_3g081090 56.383 282 96 4 1 265 1 272 1.64e-93 279
MsG0480023711.01.T01 MTR_3g081090 51.765 255 96 4 1 238 1 245 1.55e-70 221
MsG0480023711.01.T01 MTR_3g081090 51.765 255 96 4 1 238 1 245 2.27e-70 222
MsG0480023711.01.T01 MTR_3g081090 46.188 223 93 4 1 206 1 213 2.05e-44 151
MsG0480023711.01.T01 MTR_1g018690 45.926 135 71 1 177 309 33 167 1.63e-34 128
MsG0480023711.01.T01 MTR_4g013760 42.553 141 79 1 181 319 37 177 3.31e-32 121
MsG0480023711.01.T01 MTR_7g070350 42.105 133 71 3 171 298 14 145 1.16e-24 102
MsG0480023711.01.T01 MTR_7g070320 48.305 118 57 2 171 285 20 136 2.02e-24 104
MsG0480023711.01.T01 MTR_3g012490 30.729 192 124 2 135 324 193 377 2.04e-19 89.4
MsG0480023711.01.T01 MTR_4g084080 37.121 132 77 2 181 310 17 144 5.26e-19 88.6
MsG0480023711.01.T01 MTR_2g066940 34.437 151 88 3 166 309 101 247 6.09e-19 87.8
MsG0480023711.01.T01 MTR_2g067150 36.434 129 76 2 183 309 161 285 8.70e-19 87.4
MsG0480023711.01.T01 MTR_3g098590 36.567 134 70 3 181 309 18 141 1.52e-17 83.6
MsG0480023711.01.T01 MTR_3g012500 34.815 135 79 3 177 309 306 433 3.22e-16 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023711.01.T01 AT5G42050 59.437 355 95 9 1 313 1 348 7.03e-121 352
MsG0480023711.01.T01 AT3G27090 52.090 311 119 8 10 311 3 292 4.90e-96 286
MsG0480023711.01.T01 AT3G27090 52.090 311 119 8 10 311 3 292 4.90e-96 286
MsG0480023711.01.T01 AT3G27090 52.090 311 119 8 10 311 3 292 4.90e-96 286
MsG0480023711.01.T01 AT5G01660 41.667 120 67 2 181 298 18 136 5.55e-25 105
MsG0480023711.01.T01 AT5G01660 41.667 120 67 2 181 298 18 136 7.78e-25 105
MsG0480023711.01.T01 AT5G01660 41.667 120 67 2 181 298 18 136 7.78e-25 105
MsG0480023711.01.T01 AT3G11000 42.424 132 73 2 181 310 11 141 2.81e-24 103
MsG0480023711.01.T01 AT3G11000 42.424 132 73 2 181 310 130 260 7.42e-24 102
MsG0480023711.01.T01 AT5G61910 37.500 136 77 2 175 309 55 183 2.08e-22 98.6
MsG0480023711.01.T01 AT5G61910 37.500 136 77 2 175 309 55 183 2.08e-22 98.6
MsG0480023711.01.T01 AT5G61910 37.500 136 77 2 175 309 59 187 2.33e-22 98.2
MsG0480023711.01.T01 AT5G61910 37.500 136 77 2 175 309 55 183 2.95e-22 97.8
MsG0480023711.01.T01 AT5G61910 37.500 136 77 2 175 309 55 183 5.90e-21 94.4
MsG0480023711.01.T01 AT2G35140 34.722 144 88 3 183 324 23 162 9.37e-19 87.8
MsG0480023711.01.T01 AT2G35140 34.722 144 88 3 183 324 23 162 9.37e-19 87.8
MsG0480023711.01.T01 AT2G35140 34.722 144 88 3 183 324 23 162 9.37e-19 87.8
MsG0480023711.01.T01 AT2G35140 34.722 144 88 3 183 324 23 162 9.37e-19 87.8
MsG0480023711.01.T01 AT2G35140 34.722 144 88 3 183 324 23 162 9.37e-19 87.8
MsG0480023711.01.T01 AT2G35140 34.722 144 88 3 183 324 49 188 9.73e-19 87.4
MsG0480023711.01.T01 AT2G32910 29.536 237 141 9 81 309 224 442 4.02e-18 85.5

Find 82 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGTTGTGTCAGCATAAA+AGG 0.132335 4:-88854400 MsG0480023711.01.T01:exon
CCTAACTGAATCCCTGTATC+TGG 0.267819 4:+88855241 None:intergenic
TGGTATAGTTGTAAAGAAAA+AGG 0.278735 4:+88855198 None:intergenic
GTTGGTGTTTCTCCTATTAA+TGG 0.279410 4:-88856630 MsG0480023711.01.T01:CDS
TTATTAAAACCTCCATTAAT+AGG 0.287108 4:+88856618 None:intergenic
ATACTAGATAATTAGAGATT+TGG 0.288893 4:-88854317 MsG0480023711.01.T01:three_prime_UTR
ATTTATTCAAAGCCTTCTTA+TGG 0.312416 4:-88856591 MsG0480023711.01.T01:CDS
TCAGTTAGGGCCATTACTCC+TGG 0.331278 4:-88855227 MsG0480023711.01.T01:CDS
ATTAGGGTTTGAATTCTTCT+TGG 0.332710 4:+88856500 None:intergenic
TATCTCTTAATGATTCAATC+TGG 0.342706 4:-88856818 MsG0480023711.01.T01:CDS
TACTAGATAATTAGAGATTT+GGG 0.346604 4:-88854316 MsG0480023711.01.T01:three_prime_UTR
AGATTGAATCATTAAGAGAT+AGG 0.365054 4:+88856820 None:intergenic
TAGGTTTGCCTCCCAGATAC+AGG 0.367382 4:-88855253 MsG0480023711.01.T01:CDS
CCAGATACAGGGATTCAGTT+AGG 0.380760 4:-88855241 MsG0480023711.01.T01:CDS
CCACCAATGGTTTCATTCCT+AGG 0.384980 4:+88856375 None:intergenic
TTCTTCTTGGAAGATTTAGA+TGG 0.386595 4:+88856513 None:intergenic
AACAACAACTCTGTTTCTCT+TGG 0.386776 4:-88856711 MsG0480023711.01.T01:CDS
TTATTGAAATGATCAGAAAC+AGG 0.392314 4:+88856681 None:intergenic
TTTGTTTAGGCTGCAAGTTT+TGG 0.392417 4:-88854945 MsG0480023711.01.T01:intron
CAAATTACTACAAAGAATAA+AGG 0.401147 4:-88854366 MsG0480023711.01.T01:three_prime_UTR
TCTGATCATTTCAATAATCA+AGG 0.410681 4:-88856675 MsG0480023711.01.T01:CDS
AATCTCAAAAGGCAACTCTT+TGG 0.419984 4:-88856315 MsG0480023711.01.T01:intron
CCATCATAGTGGTGAAGAAC+TGG 0.427123 4:+88854610 None:intergenic
TGCTTCCAACGACACTTTCA+AGG 0.433408 4:-88854418 MsG0480023711.01.T01:CDS
ATCTCTTAATGATTCAATCT+GGG 0.445296 4:-88856817 MsG0480023711.01.T01:CDS
TTAAAATTCCCACCATAAGA+AGG 0.446938 4:+88856579 None:intergenic
AAATATTGATCCAACGGCTT+GGG 0.453134 4:-88854916 MsG0480023711.01.T01:CDS
CACTAGAAGAGGATTCATTC+AGG 0.455058 4:-88854633 MsG0480023711.01.T01:CDS
CGAATCTCGTTTCCCTGCTC+AGG 0.457693 4:-88854874 MsG0480023711.01.T01:intron
TACAGGTAAGAGTGCTTACT+AGG 0.458108 4:-88854669 MsG0480023711.01.T01:intron
TTAATATATAGATATAGATA+TGG 0.459568 4:+88857074 None:intergenic
TCTCTTGTCAGCAGCCGCCT+TGG 0.468255 4:+88856428 None:intergenic
TTCTTGTCCTCCCAAGCCGT+TGG 0.480092 4:+88854906 None:intergenic
CAGTTAGGGCCATTACTCCT+GGG 0.491247 4:-88855226 MsG0480023711.01.T01:CDS
AATGAAAATGATACCTGAGC+AGG 0.497887 4:+88854861 None:intergenic
AAGGTTGTGTCAGCATAAAA+GGG 0.500936 4:-88854399 MsG0480023711.01.T01:exon
AGCTCTATAACACAATATAT+AGG 0.508792 4:+88854211 None:intergenic
GTTGTAGGCATTGTCTCTGC+TGG 0.511637 4:-88854460 MsG0480023711.01.T01:intron
CAGATACAGGGATTCAGTTA+GGG 0.515717 4:-88855240 MsG0480023711.01.T01:CDS
CCAGTTCTTCACCACTATGA+TGG 0.516230 4:-88854610 MsG0480023711.01.T01:CDS
TGGGAGGACAAGAAGTGTGC+TGG 0.518213 4:-88854897 MsG0480023711.01.T01:CDS
TTTATATATTAATATAGCTA+CGG 0.522521 4:-88854287 MsG0480023711.01.T01:three_prime_UTR
TATTCAAAGCCTTCTTATGG+TGG 0.524188 4:-88856588 MsG0480023711.01.T01:CDS
CAAATATTGATCCAACGGCT+TGG 0.524783 4:-88854917 MsG0480023711.01.T01:CDS
CTAACTGAATCCCTGTATCT+GGG 0.538745 4:+88855242 None:intergenic
AAGGGGCAACCCAGGAGTAA+TGG 0.541588 4:+88855217 None:intergenic
ATTCAAAGCCTTCTTATGGT+GGG 0.541890 4:-88856587 MsG0480023711.01.T01:CDS
TATTGATCCAACGGCTTGGG+AGG 0.545418 4:-88854913 MsG0480023711.01.T01:CDS
GGTGTTTCTCCTATTAATGG+AGG 0.546103 4:-88856627 MsG0480023711.01.T01:CDS
CACAACCTTGAAAGTGTCGT+TGG 0.548994 4:+88854413 None:intergenic
AGTAACATGAATGTTACTAA+AGG 0.551944 4:-88856555 MsG0480023711.01.T01:CDS
CTGAATGAATCCTCTTCTAG+TGG 0.554234 4:+88854634 None:intergenic
CCATGGCTGAGAATCTCAAA+AGG 0.555325 4:-88856326 MsG0480023711.01.T01:CDS
AGGTTTGCCTCCCAGATACA+GGG 0.571707 4:-88855252 MsG0480023711.01.T01:CDS
CACCAGCAGAGTCTCTTCCT+AGG 0.573046 4:-88856392 MsG0480023711.01.T01:CDS
GGGGAGAACAATAACAGTGA+TGG 0.573073 4:-88856468 MsG0480023711.01.T01:CDS
CCTAGGAATGAAACCATTGG+TGG 0.576885 4:-88856375 MsG0480023711.01.T01:CDS
TTCAGCTCAAGACGGAACTT+AGG 0.577473 4:+88854586 None:intergenic
GGAACTTAGGTCCATCATAG+TGG 0.580135 4:+88854599 None:intergenic
TATACCACTCACCAACTCCA+TGG 0.580365 4:-88855182 MsG0480023711.01.T01:CDS
CTTCCTAGGAATGAAACCAT+TGG 0.584075 4:-88856378 MsG0480023711.01.T01:CDS
TTCCTAGGAAGAGACTCTGC+TGG 0.585025 4:+88856390 None:intergenic
AACATGTGAGCCACTAGAAG+AGG 0.589083 4:-88854644 MsG0480023711.01.T01:CDS
ATGAAAATGATACCTGAGCA+GGG 0.598544 4:+88854862 None:intergenic
GGTTTGCTAGATTTGATGTG+TGG 0.599698 4:+88856841 None:intergenic
TCTCTTAATGATTCAATCTG+GGG 0.606044 4:-88856816 MsG0480023711.01.T01:CDS
TGGAACAAATATTGATCCAA+CGG 0.610123 4:-88854922 MsG0480023711.01.T01:CDS
AAATAAGAACAAGGATTCCA+AGG 0.613003 4:-88856445 MsG0480023711.01.T01:CDS
ATATACACATACACATAGGT+AGG 0.614919 4:-88856947 MsG0480023711.01.T01:five_prime_UTR
CAGTGATGGAAATAAGAACA+AGG 0.622810 4:-88856454 MsG0480023711.01.T01:CDS
AACTCCATGGAGTTGGTGAG+TGG 0.632771 4:+88855178 None:intergenic
TATTGAAATGATCAGAAACA+GGG 0.638940 4:+88856682 None:intergenic
CTAGGAATGAAACCATTGGT+GGG 0.642582 4:-88856374 MsG0480023711.01.T01:CDS
GACAAAGATATACCCACCAA+TGG 0.644157 4:+88856362 None:intergenic
CTAGGAAGAGACTCTGCTGG+TGG 0.665578 4:+88856393 None:intergenic
CAGGCACATTCAGCTCAAGA+CGG 0.668323 4:+88854578 None:intergenic
TCTTGAGCTGAATGTGCCTG+AGG 0.674355 4:-88854575 MsG0480023711.01.T01:intron
TTACATATACACATACACAT+AGG 0.682964 4:-88856951 MsG0480023711.01.T01:five_prime_UTR
TGTCTGTAACAATGATACCA+TGG 0.683554 4:-88856343 MsG0480023711.01.T01:CDS
ATTGAAATGATCAGAAACAG+GGG 0.684933 4:+88856683 None:intergenic
ACTGAATCCCTGTATCTGGG+AGG 0.733702 4:+88855245 None:intergenic
TAAGAACAAGGATTCCAAGG+CGG 0.748891 4:-88856442 MsG0480023711.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTAAAAAAAAAAGG+GGG + Chr4:88856687-88856706 None:intergenic 10.0%
!! ATAATATATTTACTATAGAT+TGG - Chr4:88855780-88855799 MsG0480023711.01.T01:intron 10.0%
!! GAAATAATTAAAAAAAAAAG+GGG + Chr4:88856688-88856707 None:intergenic 10.0%
!! TAAACAAAATTAAAAAAATG+GGG + Chr4:88855330-88855349 None:intergenic 10.0%
!! TTAATATATAGATATAGATA+TGG + Chr4:88854128-88854147 None:intergenic 10.0%
!! TTTATATATTAATATAGCTA+CGG - Chr4:88856912-88856931 MsG0480023711.01.T01:exon 10.0%
!!! AAAAATTTTTTAAGGTAAAA+AGG - Chr4:88855061-88855080 MsG0480023711.01.T01:intron 10.0%
!!! TTTTTGACAAAAATTTTTTA+AGG - Chr4:88855053-88855072 MsG0480023711.01.T01:intron 10.0%
!!! AATTGTTTTTTACATATCTA+CGG + Chr4:88856953-88856972 None:intergenic 15.0%
!!! ATAAGTTAAAGTGTAATTTT+GGG - Chr4:88855360-88855379 MsG0480023711.01.T01:intron 15.0%
!!! ATTATTTCCCTTTTTTTTTT+TGG - Chr4:88854998-88855017 MsG0480023711.01.T01:intron 15.0%
!!! TATAAGTTAAAGTGTAATTT+TGG - Chr4:88855359-88855378 MsG0480023711.01.T01:intron 15.0%
!!! TGAGTTTTTATTTGAAAATT+CGG - Chr4:88855160-88855179 MsG0480023711.01.T01:intron 15.0%
!! AAAAACATAATTGATACTGA+AGG + Chr4:88855901-88855920 None:intergenic 20.0%
!! AATAAGAATTGCATAATTGA+AGG + Chr4:88854938-88854957 None:intergenic 20.0%
!! AATCGAACCAAAAAAAAAAA+GGG + Chr4:88855008-88855027 None:intergenic 20.0%
!! ATAATTGAAGGAAACAATTT+AGG + Chr4:88854926-88854945 None:intergenic 20.0%
!! ATACTAGATAATTAGAGATT+TGG - Chr4:88856882-88856901 MsG0480023711.01.T01:CDS 20.0%
!! CAAAATGTATTCAAATTATG+AGG + Chr4:88856177-88856196 None:intergenic 20.0%
!! CAAATTACTACAAAGAATAA+AGG - Chr4:88856833-88856852 MsG0480023711.01.T01:CDS 20.0%
!! GTAAATATATTATGCTTCTA+TGG + Chr4:88855773-88855792 None:intergenic 20.0%
!! TAATCGAACCAAAAAAAAAA+AGG + Chr4:88855009-88855028 None:intergenic 20.0%
!! TACTAGATAATTAGAGATTT+GGG - Chr4:88856883-88856902 MsG0480023711.01.T01:CDS 20.0%
!! TATGTAAAAAACAATTTGAG+AGG - Chr4:88856957-88856976 MsG0480023711.01.T01:five_prime_UTR 20.0%
!! TCTTTAACATTGATAAATAC+AGG - Chr4:88856513-88856532 MsG0480023711.01.T01:CDS 20.0%
!! TTATTAAAACCTCCATTAAT+AGG + Chr4:88854584-88854603 None:intergenic 20.0%
!! TTATTTGAAAATTCGGTATT+CGG - Chr4:88855167-88855186 MsG0480023711.01.T01:intron 20.0%
!!! AAAAAGATTGTTGATTGTTA+TGG + Chr4:88854313-88854332 None:intergenic 20.0%
!!! AAAATTTCTCCTTTGATTTT+TGG + Chr4:88854426-88854445 None:intergenic 20.0%
!!! AATTTTACTTCTGTCTTAAA+TGG + Chr4:88855228-88855247 None:intergenic 20.0%
!!! AGAAATTTTGATACAAAACT+TGG - Chr4:88854437-88854456 MsG0480023711.01.T01:CDS 20.0%
!!! ATAACAATCAACAATCTTTT+TGG - Chr4:88854312-88854331 MsG0480023711.01.T01:three_prime_UTR 20.0%
!!! ATTAATGGAGGTTTTAATAA+GGG - Chr4:88854584-88854603 MsG0480023711.01.T01:CDS 20.0%
!!! ATTTTACTTCTGTCTTAAAT+GGG + Chr4:88855227-88855246 None:intergenic 20.0%
!!! ATTTTCGAAGTAATTTATTC+CGG + Chr4:88855418-88855437 None:intergenic 20.0%
!!! CACAATTTTAATGGATAATT+CGG + Chr4:88856124-88856143 None:intergenic 20.0%
!!! CTCATAATTTGAATACATTT+TGG - Chr4:88856175-88856194 MsG0480023711.01.T01:intron 20.0%
!!! TATTAATGGAGGTTTTAATA+AGG - Chr4:88854583-88854602 MsG0480023711.01.T01:CDS 20.0%
!!! TTTCAAGTTTTGTGAAAAAA+TGG - Chr4:88854280-88854299 MsG0480023711.01.T01:three_prime_UTR 20.0%
! AAACCCTAATAAAAAACAAG+GGG - Chr4:88854712-88854731 MsG0480023711.01.T01:intron 25.0%
! AACAATAACTGCAAAATTTC+AGG + Chr4:88855744-88855763 None:intergenic 25.0%
! AATGTCAATTGCAATATTTG+AGG - Chr4:88855844-88855863 MsG0480023711.01.T01:intron 25.0%
! AGATTGAATCATTAAGAGAT+AGG + Chr4:88854382-88854401 None:intergenic 25.0%
! AGCTCTATAACACAATATAT+AGG + Chr4:88856991-88857010 None:intergenic 25.0%
! AGTAACATGAATGTTACTAA+AGG - Chr4:88854644-88854663 MsG0480023711.01.T01:CDS 25.0%
! ATAATATGGATTTAACTACC+CGG - Chr4:88855396-88855415 MsG0480023711.01.T01:intron 25.0%
! ATCCATTAAAATTGTGCATT+AGG - Chr4:88856128-88856147 MsG0480023711.01.T01:intron 25.0%
! ATCCCAAACAAAAGAAAAAT+TGG + Chr4:88857029-88857048 None:intergenic 25.0%
! ATCTCTTAATGATTCAATCT+GGG - Chr4:88854382-88854401 MsG0480023711.01.T01:three_prime_UTR 25.0%
! ATTTATGAGACTGTTAGTTA+GGG - Chr4:88856095-88856114 MsG0480023711.01.T01:intron 25.0%
! ATTTATTCAAAGCCTTCTTA+TGG - Chr4:88854608-88854627 MsG0480023711.01.T01:CDS 25.0%
! CAAACCCTAATAAAAAACAA+GGG - Chr4:88854711-88854730 MsG0480023711.01.T01:intron 25.0%
! GGTATAGTTGTAAAGAAAAA+GGG + Chr4:88856003-88856022 None:intergenic 25.0%
! GTATAGTTGTAAAGAAAAAG+GGG + Chr4:88856002-88856021 None:intergenic 25.0%
! TATCTCTTAATGATTCAATC+TGG - Chr4:88854381-88854400 MsG0480023711.01.T01:three_prime_UTR 25.0%
! TATTGAAATGATCAGAAACA+GGG + Chr4:88854520-88854539 None:intergenic 25.0%
! TCAAACCCTAATAAAAAACA+AGG - Chr4:88854710-88854729 MsG0480023711.01.T01:intron 25.0%
! TCTGATCATTTCAATAATCA+AGG - Chr4:88854524-88854543 MsG0480023711.01.T01:intron 25.0%
! TGGTATAGTTGTAAAGAAAA+AGG + Chr4:88856004-88856023 None:intergenic 25.0%
! TTACATATACACATACACAT+AGG - Chr4:88854248-88854267 MsG0480023711.01.T01:three_prime_UTR 25.0%
! TTATTGAAATGATCAGAAAC+AGG + Chr4:88854521-88854540 None:intergenic 25.0%
!! TAAACTGTGCTTTCTTTTAT+GGG - Chr4:88856717-88856736 MsG0480023711.01.T01:CDS 25.0%
!! TGTCCAATTTTTCTTTTGTT+TGG - Chr4:88857023-88857042 MsG0480023711.01.T01:five_prime_UTR 25.0%
!! TTATATATGAACTAGTCTAC+TGG - Chr4:88855246-88855265 MsG0480023711.01.T01:CDS 25.0%
!! TTGTGAATGTTTGTGTTTTT+AGG - Chr4:88855927-88855946 MsG0480023711.01.T01:intron 25.0%
!! TTTTCGAAGTAATTTATTCC+GGG + Chr4:88855417-88855436 None:intergenic 25.0%
!!! AATTTTGATACAAAACTTGG+TGG - Chr4:88854440-88854459 MsG0480023711.01.T01:CDS 25.0%
!!! GTCCAATTTTTCTTTTGTTT+GGG - Chr4:88857024-88857043 MsG0480023711.01.T01:five_prime_UTR 25.0%
!!! TCTTTTTTCTCTTTTGATGT+TGG - Chr4:88854551-88854570 MsG0480023711.01.T01:intron 25.0%
!!! TTATTTTGGTTACCATGTAT+GGG - Chr4:88855662-88855681 MsG0480023711.01.T01:intron 25.0%
!!! TTTATTTTGGTTACCATGTA+TGG - Chr4:88855661-88855680 MsG0480023711.01.T01:intron 25.0%
AAATAAGAACAAGGATTCCA+AGG - Chr4:88854754-88854773 MsG0480023711.01.T01:intron 30.0%
ACAGGTTATAATCCCTTATA+TGG + Chr4:88855814-88855833 None:intergenic 30.0%
ACAGTCTCATAAATGTGATT+AGG + Chr4:88856089-88856108 None:intergenic 30.0%
ATATACACATACACATAGGT+AGG - Chr4:88854252-88854271 MsG0480023711.01.T01:three_prime_UTR 30.0%
ATGTATTCAAATTATGAGGC+TGG + Chr4:88856173-88856192 None:intergenic 30.0%
ATTAGGGTTTGAATTCTTCT+TGG + Chr4:88854702-88854721 None:intergenic 30.0%
ATTGAAATGATCAGAAACAG+GGG + Chr4:88854519-88854538 None:intergenic 30.0%
CATTTATGAGACTGTTAGTT+AGG - Chr4:88856094-88856113 MsG0480023711.01.T01:intron 30.0%
GGAAACAATTTAGGAAAGAA+AGG + Chr4:88854917-88854936 None:intergenic 30.0%
GTTTAGCTACTCGAATAATA+TGG - Chr4:88855382-88855401 MsG0480023711.01.T01:intron 30.0%
TCTCTTAATGATTCAATCTG+GGG - Chr4:88854383-88854402 MsG0480023711.01.T01:three_prime_UTR 30.0%
TGATATCAATATCACTGTAC+TGG - Chr4:88855686-88855705 MsG0480023711.01.T01:intron 30.0%
TGGAACAAATATTGATCCAA+CGG - Chr4:88856277-88856296 MsG0480023711.01.T01:intron 30.0%
TTAAAATTCCCACCATAAGA+AGG + Chr4:88854623-88854642 None:intergenic 30.0%
TTCTTCTTGGAAGATTTAGA+TGG + Chr4:88854689-88854708 None:intergenic 30.0%
TTGACATTGAATTGAAGAAC+AGG + Chr4:88855832-88855851 None:intergenic 30.0%
! AAGATAGCCAAATTTTGTTG+AGG + Chr4:88855597-88855616 None:intergenic 30.0%
! AGATAGCCAAATTTTGTTGA+GGG + Chr4:88855596-88855615 None:intergenic 30.0%
! CTAAACTGTGCTTTCTTTTA+TGG - Chr4:88856716-88856735 MsG0480023711.01.T01:CDS 30.0%
! TGCCTAATGCACAATTTTAA+TGG + Chr4:88856133-88856152 None:intergenic 30.0%
!! ATTTCTCCTTTGATTTTTGG+AGG + Chr4:88854423-88854442 None:intergenic 30.0%
!! TTCTCTATGTACTTGCTATA+GGG + Chr4:88855517-88855536 None:intergenic 30.0%
!!! GTCTATCTGTAGCTTTATTT+TGG - Chr4:88855648-88855667 MsG0480023711.01.T01:intron 30.0%
!!! TATTTTGGTTACCATGTATG+GGG - Chr4:88855663-88855682 MsG0480023711.01.T01:intron 30.0%
!!! TCTCCCCTTGTTTTTTATTA+GGG + Chr4:88854718-88854737 None:intergenic 30.0%
!!! TGTTTTGATCTTCTAGTTTG+TGG - Chr4:88856474-88856493 MsG0480023711.01.T01:CDS 30.0%
!!! TTCTCCCCTTGTTTTTTATT+AGG + Chr4:88854719-88854738 None:intergenic 30.0%
!!! TTTTTGAGGTATGTGTTACT+TGG - Chr4:88856042-88856061 MsG0480023711.01.T01:intron 30.0%
AAATATTGATCCAACGGCTT+GGG - Chr4:88856283-88856302 MsG0480023711.01.T01:intron 35.0%
AACAGAGAACAAAAAGCAGT+TGG + Chr4:88854210-88854229 None:intergenic 35.0%
AAGGTTGTGTCAGCATAAAA+GGG - Chr4:88856800-88856819 MsG0480023711.01.T01:CDS 35.0%
AATCAGATAACATATACCCC+CGG + Chr4:88855628-88855647 None:intergenic 35.0%
AATCTCAAAAGGCAACTCTT+TGG - Chr4:88854884-88854903 MsG0480023711.01.T01:CDS 35.0%
AATGAAAATGATACCTGAGC+AGG + Chr4:88856341-88856360 None:intergenic 35.0%
ACTCATCCTCCAAAAATCAA+AGG - Chr4:88854414-88854433 MsG0480023711.01.T01:CDS 35.0%
ATGAAAATGATACCTGAGCA+GGG + Chr4:88856340-88856359 None:intergenic 35.0%
ATTCAAAGCCTTCTTATGGT+GGG - Chr4:88854612-88854631 MsG0480023711.01.T01:CDS 35.0%
ATTGGTGTGTTAGCCATATA+AGG - Chr4:88855798-88855817 MsG0480023711.01.T01:intron 35.0%
TAATGTTACGTCGTAACATG+TGG - Chr4:88856219-88856238 MsG0480023711.01.T01:intron 35.0%
TATTCAAAGCCTTCTTATGG+TGG - Chr4:88854611-88854630 MsG0480023711.01.T01:CDS 35.0%
TATTGATATCACCCCATACA+TGG + Chr4:88855677-88855696 None:intergenic 35.0%
TGTCTGTAACAATGATACCA+TGG - Chr4:88854856-88854875 MsG0480023711.01.T01:intron 35.0%
TTGGTGTGTTAGCCATATAA+GGG - Chr4:88855799-88855818 MsG0480023711.01.T01:intron 35.0%
! AACAACAACTCTGTTTCTCT+TGG - Chr4:88854488-88854507 MsG0480023711.01.T01:intron 35.0%
! ATAGCCAAATTTTGTTGAGG+GGG + Chr4:88855594-88855613 None:intergenic 35.0%
! CAGTGATGGAAATAAGAACA+AGG - Chr4:88854745-88854764 MsG0480023711.01.T01:intron 35.0%
! CATTTTCATTCCAACCTGTA+CGG - Chr4:88856351-88856370 MsG0480023711.01.T01:CDS 35.0%
! GATAGCCAAATTTTGTTGAG+GGG + Chr4:88855595-88855614 None:intergenic 35.0%
! GTTGGTGTTTCTCCTATTAA+TGG - Chr4:88854569-88854588 MsG0480023711.01.T01:intron 35.0%
! TTTGTTTAGGCTGCAAGTTT+TGG - Chr4:88856254-88856273 MsG0480023711.01.T01:intron 35.0%
!! GTTCTCTATGTACTTGCTAT+AGG + Chr4:88855518-88855537 None:intergenic 35.0%
!! TGCTTTCTTTTATGGGTTGT+AGG - Chr4:88856724-88856743 MsG0480023711.01.T01:CDS 35.0%
AATTGACACGAGTGTATGAG+TGG - Chr4:88855269-88855288 MsG0480023711.01.T01:intron 40.0%
AGTGAACGATCAAATACCTC+AGG + Chr4:88856643-88856662 None:intergenic 40.0%
CAAATATTGATCCAACGGCT+TGG - Chr4:88856282-88856301 MsG0480023711.01.T01:intron 40.0%
CAAGGTTGTGTCAGCATAAA+AGG - Chr4:88856799-88856818 MsG0480023711.01.T01:CDS 40.0%
CACATACCTCAAAAACTCCA+TGG + Chr4:88856037-88856056 None:intergenic 40.0%
CACTAGAAGAGGATTCATTC+AGG - Chr4:88856566-88856585 MsG0480023711.01.T01:CDS 40.0%
CACTTGCGCGTATTGAATTA+TGG - Chr4:88856659-88856678 MsG0480023711.01.T01:CDS 40.0%
CAGATACAGGGATTCAGTTA+GGG - Chr4:88855959-88855978 MsG0480023711.01.T01:intron 40.0%
CTAACTGAATCCCTGTATCT+GGG + Chr4:88855960-88855979 None:intergenic 40.0%
CTAGGAATGAAACCATTGGT+GGG - Chr4:88854825-88854844 MsG0480023711.01.T01:intron 40.0%
CTGAATGAATCCTCTTCTAG+TGG + Chr4:88856568-88856587 None:intergenic 40.0%
CTTCCTAGGAATGAAACCAT+TGG - Chr4:88854821-88854840 MsG0480023711.01.T01:intron 40.0%
GACAAAGATATACCCACCAA+TGG + Chr4:88854840-88854859 None:intergenic 40.0%
GACGAGACAGTAGATTTAGA+CGG - Chr4:88856407-88856426 MsG0480023711.01.T01:CDS 40.0%
GGTGTTTCTCCTATTAATGG+AGG - Chr4:88854572-88854591 MsG0480023711.01.T01:intron 40.0%
TAAGAACAAGGATTCCAAGG+CGG - Chr4:88854757-88854776 MsG0480023711.01.T01:intron 40.0%
TGTCTCGTCTCTACTCTTTA+CGG + Chr4:88856396-88856415 None:intergenic 40.0%
TTAAAAAAATGGGGCAGCCA+AGG + Chr4:88855321-88855340 None:intergenic 40.0%
! TGAAGCATAGTCTTAGCTTG+CGG - Chr4:88855292-88855311 MsG0480023711.01.T01:intron 40.0%
!! GGTTTGCTAGATTTGATGTG+TGG + Chr4:88854361-88854380 None:intergenic 40.0%
!! TACAGGTAAGAGTGCTTACT+AGG - Chr4:88856530-88856549 MsG0480023711.01.T01:CDS 40.0%
AACATGTGAGCCACTAGAAG+AGG - Chr4:88856555-88856574 MsG0480023711.01.T01:CDS 45.0%
CATACTAAAGCCGTACAGGT+TGG + Chr4:88856364-88856383 None:intergenic 45.0%
CCACCAATGGTTTCATTCCT+AGG + Chr4:88854827-88854846 None:intergenic 45.0%
CCAGATACAGGGATTCAGTT+AGG - Chr4:88855958-88855977 MsG0480023711.01.T01:intron 45.0%
CCAGTTCTTCACCACTATGA+TGG - Chr4:88856589-88856608 MsG0480023711.01.T01:CDS 45.0%
CCATCATAGTGGTGAAGAAC+TGG + Chr4:88856592-88856611 None:intergenic 45.0%
CCATGGCTGAGAATCTCAAA+AGG - Chr4:88854873-88854892 MsG0480023711.01.T01:intron 45.0%
CCTAACTGAATCCCTGTATC+TGG + Chr4:88855961-88855980 None:intergenic 45.0%
CCTAGGAATGAAACCATTGG+TGG - Chr4:88854824-88854843 MsG0480023711.01.T01:intron 45.0%
CCTCAAAAACTCCATGGAGT+TGG + Chr4:88856031-88856050 None:intergenic 45.0%
GGAACTTAGGTCCATCATAG+TGG + Chr4:88856603-88856622 None:intergenic 45.0%
GGGGAGAACAATAACAGTGA+TGG - Chr4:88854731-88854750 MsG0480023711.01.T01:intron 45.0%
GTGGGGTCGTTTGTTTGTTT+AGG - Chr4:88856241-88856260 MsG0480023711.01.T01:intron 45.0%
GTTACGTCGTAACATGTGGT+GGG - Chr4:88856223-88856242 MsG0480023711.01.T01:intron 45.0%
TAAAGAAAAAGGGGCAACCC+AGG + Chr4:88855993-88856012 None:intergenic 45.0%
TATACCACTCACCAACTCCA+TGG - Chr4:88856017-88856036 MsG0480023711.01.T01:intron 45.0%
TGCTTCCAACGACACTTTCA+AGG - Chr4:88856781-88856800 MsG0480023711.01.T01:CDS 45.0%
TGTCTTAAATGGGCCCTCAA+GGG + Chr4:88855217-88855236 None:intergenic 45.0%
TGTTACGTCGTAACATGTGG+TGG - Chr4:88856222-88856241 MsG0480023711.01.T01:intron 45.0%
TTACGTCGTAACATGTGGTG+GGG - Chr4:88856224-88856243 MsG0480023711.01.T01:intron 45.0%
TTCAGCTCAAGACGGAACTT+AGG + Chr4:88856616-88856635 None:intergenic 45.0%
TTGGCCCCCTCAACAAAATT+TGG - Chr4:88855587-88855606 MsG0480023711.01.T01:intron 45.0%
! CAAAAAGCAGTTGGTGTGTG+TGG + Chr4:88854201-88854220 None:intergenic 45.0%
! CAACCTGTACGGCTTTAGTA+TGG - Chr4:88856362-88856381 MsG0480023711.01.T01:CDS 45.0%
! CCAACTCCATGGAGTTTTTG+AGG - Chr4:88856028-88856047 MsG0480023711.01.T01:intron 45.0%
! GTATTGATGCAGCAGCCAAT+GGG + Chr4:88856439-88856458 None:intergenic 45.0%
! TGTATTGATGCAGCAGCCAA+TGG + Chr4:88856440-88856459 None:intergenic 45.0%
!! CACAACCTTGAAAGTGTCGT+TGG + Chr4:88856789-88856808 None:intergenic 45.0%
!! GTTTAGGCTGCAAGTTTTGG+TGG - Chr4:88856257-88856276 MsG0480023711.01.T01:intron 45.0%
!!! CCTTTTGAGATTCTCAGCCA+TGG + Chr4:88854876-88854895 None:intergenic 45.0%
!! ATGAAATAATTAAAAAAAAA+AGG + Chr4:88856690-88856709 None:intergenic 5.0%
!! ATTAAACAAAATTAAAAAAA+TGG + Chr4:88855332-88855351 None:intergenic 5.0%
!! TGAAATAATTAAAAAAAAAA+GGG + Chr4:88856689-88856708 None:intergenic 5.0%
!! TTAAACAAAATTAAAAAAAT+GGG + Chr4:88855331-88855350 None:intergenic 5.0%
!!! AATAATATACATTTTTTAAT+TGG + Chr4:88855118-88855137 None:intergenic 5.0%
!!! TTTTATTTTTTCTATATATT+TGG - Chr4:88855568-88855587 MsG0480023711.01.T01:intron 5.0%
ACTGAATCCCTGTATCTGGG+AGG + Chr4:88855957-88855976 None:intergenic 50.0%
AGGTTTGCCTCCCAGATACA+GGG - Chr4:88855947-88855966 MsG0480023711.01.T01:intron 50.0%
ATAACATATACCCCCGGCAG+CGG + Chr4:88855622-88855641 None:intergenic 50.0%
CAGGCACATTCAGCTCAAGA+CGG + Chr4:88856624-88856643 None:intergenic 50.0%
CAGTTAGGGCCATTACTCCT+GGG - Chr4:88855973-88855992 MsG0480023711.01.T01:intron 50.0%
CTGTCTTAAATGGGCCCTCA+AGG + Chr4:88855218-88855237 None:intergenic 50.0%
GCACCATACTAAAGCCGTAC+AGG + Chr4:88856368-88856387 None:intergenic 50.0%
TAGGTTTGCCTCCCAGATAC+AGG - Chr4:88855946-88855965 MsG0480023711.01.T01:intron 50.0%
TCAGTTAGGGCCATTACTCC+TGG - Chr4:88855972-88855991 MsG0480023711.01.T01:intron 50.0%
TCTTGAGCTGAATGTGCCTG+AGG - Chr4:88856624-88856643 MsG0480023711.01.T01:CDS 50.0%
TTAAATGGGCCCTCAAGGGT+GGG + Chr4:88855213-88855232 None:intergenic 50.0%
TTCCTAGGAAGAGACTCTGC+TGG + Chr4:88854812-88854831 None:intergenic 50.0%
! GTTGTAGGCATTGTCTCTGC+TGG - Chr4:88856739-88856758 MsG0480023711.01.T01:CDS 50.0%
! TATTGATCCAACGGCTTGGG+AGG - Chr4:88856286-88856305 MsG0480023711.01.T01:intron 50.0%
!! AACTCCATGGAGTTGGTGAG+TGG + Chr4:88856024-88856043 None:intergenic 50.0%
!! AGCATAGTCTTAGCTTGCGG+TGG - Chr4:88855295-88855314 MsG0480023711.01.T01:intron 50.0%
AAGGGGCAACCCAGGAGTAA+TGG + Chr4:88855985-88856004 None:intergenic 55.0%
CACCAGCAGAGTCTCTTCCT+AGG - Chr4:88854807-88854826 MsG0480023711.01.T01:intron 55.0%
CGAATCTCGTTTCCCTGCTC+AGG - Chr4:88856325-88856344 MsG0480023711.01.T01:CDS 55.0%
CTAGGAAGAGACTCTGCTGG+TGG + Chr4:88854809-88854828 None:intergenic 55.0%
CTTAAATGGGCCCTCAAGGG+TGG + Chr4:88855214-88855233 None:intergenic 55.0%
GGTATTCGGCCTAGTTCCGA+CGG - Chr4:88855181-88855200 MsG0480023711.01.T01:CDS 55.0%
TGATGCAGCAGCCAATGGGA+CGG + Chr4:88856435-88856454 None:intergenic 55.0%
TTCTTGTCCTCCCAAGCCGT+TGG + Chr4:88856296-88856315 None:intergenic 55.0%
TTGGCTATCTTTGCCGCTGC+CGG - Chr4:88855606-88855625 MsG0480023711.01.T01:intron 55.0%
TTTAGACGGTCCCGTCCCAT+TGG - Chr4:88856421-88856440 MsG0480023711.01.T01:CDS 55.0%
! TGGGAGGACAAGAAGTGTGC+TGG - Chr4:88856302-88856321 MsG0480023711.01.T01:intron 55.0%
ACGGTCCAATCCCACCCTTG+AGG - Chr4:88855200-88855219 MsG0480023711.01.T01:CDS 60.0%
CAAGGGTGGGATTGGACCGT+CGG + Chr4:88855200-88855219 None:intergenic 60.0%
CGGTCCAATCCCACCCTTGA+GGG - Chr4:88855201-88855220 MsG0480023711.01.T01:CDS 60.0%
GATGCAGCAGCCAATGGGAC+GGG + Chr4:88856434-88856453 None:intergenic 60.0%
GGGATTGGACCGTCGGAACT+AGG + Chr4:88855193-88855212 None:intergenic 60.0%
TCTCTTGTCAGCAGCCGCCT+TGG + Chr4:88854774-88854793 None:intergenic 60.0%
TGGCTATCTTTGCCGCTGCC+GGG - Chr4:88855607-88855626 MsG0480023711.01.T01:intron 60.0%
!! GTCTTAGCTTGCGGTGGCCT+TGG - Chr4:88855301-88855320 MsG0480023711.01.T01:intron 60.0%
GCTATCTTTGCCGCTGCCGG+GGG - Chr4:88855609-88855628 MsG0480023711.01.T01:intron 65.0%
GGCTATCTTTGCCGCTGCCG+GGG - Chr4:88855608-88855627 MsG0480023711.01.T01:intron 65.0%
TGGGCCCTCAAGGGTGGGAT+TGG + Chr4:88855208-88855227 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 88854119 88857102 88854119 ID=MsG0480023711.01;Name=MsG0480023711.01
Chr4 mRNA 88854119 88857102 88854119 ID=MsG0480023711.01.T01;Parent=MsG0480023711.01;Name=MsG0480023711.01.T01;_AED=0.35;_eAED=0.35;_QI=180|1|1|1|1|1|5|285|324
Chr4 exon 88854119 88854475 88854119 ID=MsG0480023711.01.T01:exon:25175;Parent=MsG0480023711.01.T01
Chr4 exon 88854576 88854686 88854576 ID=MsG0480023711.01.T01:exon:25174;Parent=MsG0480023711.01.T01
Chr4 exon 88854875 88854958 88854875 ID=MsG0480023711.01.T01:exon:25173;Parent=MsG0480023711.01.T01
Chr4 exon 88855172 88855272 88855172 ID=MsG0480023711.01.T01:exon:25172;Parent=MsG0480023711.01.T01
Chr4 exon 88856316 88857102 88856316 ID=MsG0480023711.01.T01:exon:25171;Parent=MsG0480023711.01.T01
Chr4 five_prime_UTR 88856923 88857102 88856923 ID=MsG0480023711.01.T01:five_prime_utr;Parent=MsG0480023711.01.T01
Chr4 CDS 88856316 88856922 88856316 ID=MsG0480023711.01.T01:cds;Parent=MsG0480023711.01.T01
Chr4 CDS 88855172 88855272 88855172 ID=MsG0480023711.01.T01:cds;Parent=MsG0480023711.01.T01
Chr4 CDS 88854875 88854958 88854875 ID=MsG0480023711.01.T01:cds;Parent=MsG0480023711.01.T01
Chr4 CDS 88854576 88854686 88854576 ID=MsG0480023711.01.T01:cds;Parent=MsG0480023711.01.T01
Chr4 CDS 88854404 88854475 88854404 ID=MsG0480023711.01.T01:cds;Parent=MsG0480023711.01.T01
Chr4 three_prime_UTR 88854119 88854403 88854119 ID=MsG0480023711.01.T01:three_prime_utr;Parent=MsG0480023711.01.T01
Gene Sequence

>MsG0480023711.01.T01

ATGGAGAACAACCATAACAATCAACAATCTTTTTGGCAATTCAGTGATCAACTTCGTGTCCACACATCAAATCTAGCAAACCTATCTCTTAATGATTCAATCTGGGGAAACAATTACTCATCCTCCAAAAATCAAAGGAGAAATTTTGATACAAAACTTGGTGGTGAAATCATCATCAACAACAACAACAACAACAACTCTGTTTCTCTTGGAAAATCCCCTGTTTCTGATCATTTCAATAATCAAGGATCATCTTTTTTCTCTTTTGATGTTGGTGTTTCTCCTATTAATGGAGGTTTTAATAAGGGAATTTATTCAAAGCCTTCTTATGGTGGGAATTTTAATAGTAACATGAATGTTACTAAAGGTGAAGATGAGATTTTTCATCCATCTAAATCTTCCAAGAAGAATTCAAACCCTAATAAAAAACAAGGGGAGAACAATAACAGTGATGGAAATAAGAACAAGGATTCCAAGGCGGCTGCTGACAAGAGATTCAAGACTTTGCCACCAGCAGAGTCTCTTCCTAGGAATGAAACCATTGGTGGGTATATCTTTGTCTGTAACAATGATACCATGGCTGAGAATCTCAAAAGGCAACTCTTTGGTTTGCCTCCCAGATACAGGGATTCAGTTAGGGCCATTACTCCTGGGTTGCCCCTTTTTCTTTACAACTATACCACTCACCAACTCCATGGAGTTTTTGAGGCTGCAAGTTTTGGTGGAACAAATATTGATCCAACGGCTTGGGAGGACAAGAAGTGTGCTGGCGAATCTCGTTTCCCTGCTCAGGTAAGAGTGCTTACTAGGAAAACATGTGAGCCACTAGAAGAGGATTCATTCAGGCCAGTTCTTCACCACTATGATGGACCTAAGTTCCGTCTTGAGCTGAATGTGCCTGAGGCATTGTCTCTGCTGGATATTTTTGAAGAACAAGATGCTTCCAACGACACTTTCAAGGTTGTGTCAGCATAA
Protein sequence

>MsG0480023711.01.T01

MENNHNNQQSFWQFSDQLRVHTSNLANLSLNDSIWGNNYSSSKNQRRNFDTKLGGEIIINNNNNNNSVSLGKSPVSDHFNNQGSSFFSFDVGVSPINGGFNKGIYSKPSYGGNFNSNMNVTKGEDEIFHPSKSSKKNSNPNKKQGENNNSDGNKNKDSKAAADKRFKTLPPAESLPRNETIGGYIFVCNNDTMAENLKRQLFGLPPRYRDSVRAITPGLPLFLYNYTTHQLHGVFEAASFGGTNIDPTAWEDKKCAGESRFPAQVRVLTRKTCEPLEEDSFRPVLHHYDGPKFRLELNVPEALSLLDIFEEQDASNDTFKVVSA*